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Pressure BioSciences, Inc.

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FY2017 Annual Report · Pressure BioSciences, Inc.
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UNITED STATES
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

Form 10-K

(Mark One)

[X] Annual Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934

For the fiscal year ended December 31, 2017 or

[  ] Transition Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934

For the transition period from __________________ to __________________

Commission file number 001-38185

PRESSURE BIOSCIENCES, INC.
(Exact Name of Registrant as Specified in its Charter)

Massachusetts
(State or Other Jurisdiction of
Incorporation or Organization)

14 Norfolk Avenue
South Easton, Massachusetts
(Address of Principal Executive Offices)

04-2652826
(I.R.S. Employer
Identification No.)

02375
(Zip Code)

(508) 230-1828

(Registrant’s Telephone Number, Including Area Code)  

Securities registered pursuant to Section 12(b) of the Act:

Title of Each Class
None

Name of Each Exchange on Which Registered
None

Securities registered pursuant to Section 12(g) of the Act:

(Title of Class)
Common Stock, par value $.01 per share

Indicate by check mark if the registrant is a well-known seasoned issuer, as defined in Rule 405 of the Securities Act.
Yes [  ] No [X]

Indicate by check mark if the registrant is not required to file reports pursuant to Section 13 or 15(d) of the Act.
Yes [  ] No [X]

Indicate by check mark whether the registrant: (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange
Act of 1934 during the preceding 12 months (or for such shorter period that the registrant was required to file such reports), and (2) has been
subject to such filing requirements for the past 90 days.

Yes [X] No [  ]

Indicate by check mark whether the registrant has submitted electronically and posted on its corporate web site, if any, every Interactive Data
File required to be submitted and posted pursuant to Rule 405 of Regulation S-T during the preceding 12 months (or for such shorter period that
registrant was required to submit and post such files.

Yes [X] No [  ]

Indicate  by  check  mark  if  disclosure  of  delinquent  filers  pursuant  to  Item  405  of  Regulation  S-K  is  not  contained  herein,  and  will  not  be
contained, to the best of registrant’s knowledge, in definitive proxy or information statements incorporated by reference in Part III of this Form
10-K or any amendment to this Form 10-K. [  ]

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, or a smaller reporting
company. See the definitions of “large accelerated filer,” “accelerated filer” and “smaller reporting company” in Rule 12b-2 of the Exchange Act.
(Check one):

Large accelerated filer [  ]
Non-accelerated filer [  ]
(Do not check if smaller reporting company)

  Accelerated filer [  ]
  Smaller reporting company [X]
  Emerging growth company [  ]

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying

with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act. [  ]

Indicate by check mark whether the registrant is a shell company (as defined in Rule 12b-2 of the Exchange Act). Yes [  ] No [X].

The  aggregate  market  value  of  the  voting  and  non-voting  common  stock  held  by  non-affiliates  of  the  registrant  as  of  June  30,  2017  was
$6,981,053 based on the closing price of $7.40 per share of Pressure BioSciences, Inc. common stock as quoted on the OTCQB Marketplace on
that date.

As of March 23, 2018, there were 1,367,852 shares of the registrant’s common stock outstanding.

Documents Incorporated by Reference

N/A.

 
 
 
 
 
 
 
 
 
TABLE OF CONTENTS

PART I

ITEM 1.

BUSINESS.

ITEM 1A. RISK FACTORS.

ITEM 1B. UNRESOLVED STAFF COMMENTS.

ITEM 2.

PROPERTIES.

ITEM 3.

LEGAL PROCEEDINGS.

ITEM 4.

MINE SAFETY DISCLOSURES

ITEM 5.

MARKET FOR REGISTRANT’S COMMON EQUITY, RELATED STOCKHOLDER MATTERS, AND ISSUER
PURCHASES OF EQUITY SECURITIES.

ITEM 6.

SELECTED FINANCIAL DATA.

PART II

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ITEM 7.

MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATION.

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ITEM 7A. QUANTITATIVE AND QUALITATIVE DISCLOSURES ABOUT MARKET RISK.

ITEM 8.

FINANCIAL STATEMENTS AND SUPPLEMENTARY DATA.

ITEM 9.

CHANGES IN AND DISAGREEMENTS WITH ACCOUNTANTS ON ACCOUNTING AND FINANCIAL
DISCLOSURE.

ITEM 9A. CONTROLS AND PROCEDURES.

ITEM 9B. OTHER INFORMATION.

ITEM 10. DIRECTORS, EXECUTIVE OFFICERS AND CORPORATE GOVERNANCE.

ITEM 11.

EXECUTIVE COMPENSATION.

PART III

ITEM 12.

SECURITY OWNERSHIP OF CERTAIN BENEFICIAL OWNERS AND MANAGEMENT AND RELATED
STOCKHOLDER MATTERS.

ITEM 13.

CERTAIN RELATIONSHIPS AND RELATED TRANSACTIONS; AND DIRECTOR INDEPENDENCE.

ITEM 14.

PRINCIPAL ACCOUNTING FEES AND SERVICES

PART IV

ITEM 15.

EXHIBITS AND FINANCIAL STATEMENT SCHEDULES.

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Introductory Comments

Throughout  this Annual  Report  on  Form  10-K,  the  terms  “we,”  “us,”  “our,”  “the  Company,”  “our  Company,”  and  “PBI,”  refer  to

Pressure BioSciences, Inc., a Massachusetts corporation, and unless the context indicates otherwise, also includes our wholly-owned subsidiary.

On June 5, 2017, the Company effected a 1-for-30 reverse stock split of its issued and outstanding shares of common stock All common
shares, stock options, and per share information presented in this Annual Report on Form 10-K have been adjusted to reflect the reverse stock
split on a retroactive basis for all periods presented.

PART I

SPECIAL NOTE REGARDING FORWARD-LOOKING STATEMENTS

This Annual Report on Form 10-K contains forward-looking statements within the meaning of Section 27A of the Securities Act of 1933, as
amended  (the  “Securities Act”)  and  Section  21E  of  the  Securities  Exchange Act  of  1934,  as  amended  (the  “Exchange Act”).  In  some  cases,
forward-looking  statements  are  identified  by  terms  such  as  “may,”  “will,”  “should,”  “could,”  “would,”  “expects,”  “plans,”  “anticipates,”
“believes,”  “estimates,”  “projects,”  “predicts,”  “potential”  and  similar  expressions  intended  to  identify  forward-looking  statements.  Such
statements include, without limitation, statements regarding:

● our need for, and our ability to raise, additional equity or debt financing on acceptable terms, if at all;
● our need  to  take  additional  cost  reduction  measures,  cease  operations  or  sell  our  operating  assets,  if  we  are  unable  to  obtain sufficient

additional financing;

● our belief that we will have sufficient liquidity to finance normal operations for the foreseeable future;
● the options we may pursue in light of our financial condition;
● the potential applications for Ultra Shearing technology;
● the potential applications of BaroFold’s PreEMT high-pressure protein refolding technology
● the amount of cash necessary to operate our business;
● the anticipated uses of grant revenue and the potential for increased grant revenue in future periods;
● our plans and expectations with respect to our continued operations;
● the expected increase in the number of pressure cycling technology (“PCT”) and constant pressure (“CP”) based units that we believe will

be installed and the expected increase in revenues from the sale of consumable products and extended service contracts;

● our belief that PCT has achieved initial market acceptance in the mass spectrometry and other markets;
● the expected development and success of new instrument and consumables product offerings;
● the potential applications for our instrument and consumables product offerings;
● the expected expenses of, and benefits and results from, our research and development efforts;
● the expected benefits and results from our collaboration programs, strategic alliances and joint ventures;
● our expectation of obtaining additional research grants from the government in the future;
● our expectations of the results of our development activities funded by government research grants;
● the potential size of the market for biological sample preparation;
● general economic conditions;
● the anticipated future financial performance and business operations of our company;
● our reasons for focusing our resources in the market for genomic, proteomic, lipidomic and small molecule sample preparation;
● the importance of mass spectrometry as a laboratory tool;
● the advantages of PCT over other current technologies as a method of biological sample preparation in biomarker discovery, forensics,  and

histology, as well as for other applications;

● the capabilities and benefits of our PCT Sample Preparation System, consumables and other products;
● our belief  that  laboratory  scientists  will  achieve  results  comparable  with  those  reported  to  date  by  certain  research  scientists who  have

published or presented publicly on PCT and our other products;

● our ability to retain our core group of scientific, administrative and sales personnel; and
● our ability to expand our customer base in sample preparation and for other applications of PCT and our other products.

These forward-looking statements are only predictions and involve known and unknown risks, uncertainties and other factors that may cause
our actual results, levels of activity, performance or achievements to be materially different from any future results, levels of activity, performance
or achievements, expressed or implied, by such forward-looking statements. Also, these forward-looking statements represent our estimates and
assumptions only as of the date of this Annual Report on Form 10-K. Except as otherwise required by law, we expressly disclaim any obligation
or undertaking to release publicly any updates or revisions to any forward-looking statement contained in this Annual Report on Form 10-K to
reflect any change in our expectations or any change in events, conditions or circumstances on which any of our forward-looking statements are
based. Factors that could cause or contribute to differences in our future financial and other results include those discussed in the risk factors set
forth  in  Part  I,  Item  1A  of  this Annual  Report  on  Form  10-K  as  well  as  those  discussed  elsewhere  in  this Annual  Report  on  Form  10-K.  We
qualify all of our forward-looking statements by these cautionary statements.

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ITEM 1. BUSINESS.

Throughout  this  document  we  use  the  following  terms:  Barocycler®,  PULSE®,  and  BioSeq®,  which  are  registered  trademarks  of  the
Company. We also use the terms ProteoSolve TM, ProteoSolveLRSTM,  the  Power  of  PCTTM,  the  PCT  ShredderTM,  HUB440TM,  HUB880TM,
micro-PestleTM, PCT-HDTM, BaroFoldTM, BarozymeTM and BaroFlexTM Strips, all of which are unregistered trademarks of the Company.

Overview

We  are  focused  on  solving  the  challenging  problems  inherent  in  biological  sample  preparation,  a  crucial  laboratory  step  performed  by
scientists worldwide working in biological life sciences research. Sample preparation is a term that refers to a wide range of activities that precede
most forms of scientific analysis. Sample preparation is often complex, time-consuming and, in our belief, one of the most error-prone steps of
scientific research. It is a widely-used laboratory undertaking – the requirements of which drive what we believe is a large and growing worldwide
market. We have developed and patented a novel, enabling technology platform that can control the sample preparation process. It is based on
harnessing  the  unique  properties  of  high  hydrostatic  pressure.  This  process,  which  we  refer  to  as  Pressure  Cycling  Technology,  or  PCT,  uses
alternating cycles of hydrostatic pressure between ambient and ultra-high levels i.e., 20,000 psi or greater to safely, conveniently and reproducibly
control the actions of molecules in biological samples, such as cells and tissues from human, animal, plant and microbial sources.

PCT is an enabling platform technology based on a physical process that had not previously been used to control bio-molecular interactions.
PCT  uses  internally  developed  instrumentation  that  is  capable  of  cycling  pressure  between  ambient  and  ultra-high  levels  at  controlled
temperatures and specific time intervals, to rapidly and repeatedly control the interactions of bio-molecules, such as proteins, DNA, RNA, lipids
and small molecules. Our laboratory instrument family, the Barocycler®, and our internally developed consumables product line, which include
our  unique  MicroTubes,  MicroCaps,  MicroPestles,  BaroFlex  and  PULSE®  (Pressure  Used  to  Lyse  Samples  for  Extraction)  Tubes,  and
application specific kits (containing consumable products and reagents), together make up our PCT Sample Preparation System (the “PCT SPS”).

In 2015, together with an investment bank, we formed a subsidiary called Pressure BioSciences Europe (“PBI Europe”) in Poland. We have
49%  ownership  interest  with  the  investment  bank  retaining  51%.  Throughout  2017,  PBI  Europe  did  not  have  any  operating  activities  and  we
cannot reasonably predict when operations will commence. Therefore, we don’t have control of the subsidiary and did not consolidate them in our
financial statements.

Patents

To date, we have been granted 15 United States and foreign patents related to our PCT technology platform, and two additional patents in
China  related  to  our  Ultra  Shear  Technology,  or  UST.  We  have  also  received  eight  patents  with  our  purchase  of  the  assets  of  BaroFold  in
December 2017. PCT employs a unique approach that we believe has the potential for broad use in a number of established and emerging life
sciences areas, which include, but are not limited to:

● biological sample  preparation  –  including  but  not  limited  to  sample  extraction,  homogenization,  and  digestion  -  in  such  study  areas as

genomic, proteomic, lipidomic, metabolomic and small molecule;

● pathogen inactivation;

● protein purification;

● control of chemical reactions, particularly enzymatic; and

● immunodiagnostics.

We  are  also  the  exclusive  distributor,  throughout  the Americas,  for  Constant  Systems,  Ltd,’s  (“CS”)  cell  disruption  equipment,  parts,  and
consumables.  CS,  a  British  company  located  several  hours  northwest  of  London,  England,  has  been  providing  niche  biomedical  equipment,
related consumable products, and services to a global client base since 1989. CS designs, develops, and manufactures high pressure cell disruption
equipment  required  by  life  sciences  laboratories  worldwide,  particularly  disruption  systems  for  the  extraction  of  proteins.  The  CS  equipment
provides  a  constant  and  controlled  cell  disruptive  environment,  giving  the  user  superior,  constant,  and  reproducible  results  whatever  the
application.  CS  has  over  900  units  installed  in  over  40  countries  worldwide.  The  CS  cell  disruption  equipment  has  proven  performance  in  the
extraction of cellular components, such as protein from yeast, bacteria, mammalian cells, and other sample types.

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The  CS  pressure-based  cell  disruption  equipment  and  our  PCT-based  instrumentation  complement  each  other  in  several  important  ways.
While both the CS and our technologies are based on high pressure, each product line has fundamental scientific capabilities that the other does
not  offer.  Our  PCT  Platform  uses  certain  patented  pressure  mechanisms  to  achieve  small-scale,  molecular  level  effects.  CS’s  technology  uses
different, proprietary pressure mechanisms for larger-scale, non-molecular level processing. In a number of routine laboratory applications, such
as  protein  extraction,  both  effects  can  be  critical  to  success.  Therefore,  for  protein  extraction  and  a  number  of  other  important  scientific
applications, we believe laboratories will benefit by using the CS and our products, either separately or together.

Primary Fields of Use and Application for PCT

Sample preparation is widely regarded as a significant impediment to research and discovery and sample extraction is generally regarded as
one  of  the  key  parts  of  sample  preparation.  The  process  of  preparing  samples  for  genomic,  proteomic,  lipidomic,  and  small  molecule  studies
includes a crucial step called sample extraction or sample disruption. This is the process of extracting biomolecules such as nucleic acid i.e., DNA
and/or  RNA,  as  well  as  proteins,  lipids,  or  small  molecules  from  the  plant  or  animal  cells  and  tissues  that  are  being  studied.  Our  current
commercialization  efforts  are  based  upon  our  belief  that  pressure  cycling  technology  provides  a  superior  solution  for  sample  extraction  when
compared  to  other  available  technologies  or  procedures  and  thus  might  significantly  improve  the  quality  of  sample  preparation,  and  thus  the
quality of the test result.

Within  the  broad  field  of  biological  sample  preparation,  in  particular  sample  extraction,  we  focus  the  majority  of  our  PCT  and  constant
pressure  (“CP”)  product  development  efforts  in  three  specific  areas:  biomarker  discovery  (primarily  through  mass  spectrometric  analysis),
forensics and histology. We believe that our existing PCT and CP-based instrumentation and related consumable products fill an important and
growing need in the sample preparation market for the safe, rapid, versatile, reproducible and quality extraction of nucleic acids, proteins, lipids,
and small molecules from a wide variety of plant, animal, and microbiological cells and tissues.

Biomarker Discovery - Mass Spectrometry

A biomarker is any substance (e.g., protein, DNA) that can be used as an indicator of the presence or absence of a particular disease-state or
condition,  and/or  to  measure  the  progression  and  effects  of  therapy.  Biomarkers  can  help  in  the  diagnosis,  prognosis,  therapy,  prevention,
surveillance, control, and cure of diseases and medical conditions.

A mass spectrometer is a laboratory instrument used in the analysis of biological samples, often focused on proteins, in life sciences research.
It  is  frequently  used  to  help  discover  biomarkers.  According  to  a  recently  published  market  report  by  Transparency  Market  Research,
“Spectrometry Market (Atomic, Molecular and Mass Spectrometry) - Global Scenario, Trends, Industry Analysis, Size, Share & Forecast 2011 –
2017,” the global spectrometry market was worth $10.2 billion in 2011 and is expected to reach $15.2 billion in 2017, growing at a compound
annual growth rate of 6.9% from 2011 to 2017. In the overall global market, the North American market is expected to maintain its lead position
in terms of revenue until 2017 and is expected to have approximately 36.2% of the market revenue share in 2017, followed next by Europe. We
believe PCT and CP-based products offer significant advantages in speed and quality compared with current techniques used in the preparation of
samples for mass spectrometry analysis.

Forensics

The detection of DNA has become a part of the analysis of forensic samples by laboratories and criminal justice agencies worldwide in their
efforts to identify the perpetrators of violent crimes and missing persons. Scientists from the University of North Texas and Florida International
University have reported improvements in DNA yield from forensic samples (e.g., bone and hair) when using the PCT platform in the sample
preparation process. We believe that PCT may be capable of differentially extracting DNA from sperm cells and female epithelial cells captured
in swabs collected from rape victims and subsequently stored in rape kits. We also believe that there are many completed rape kits that remain
untested for reasons such as cost, time and quality of results. We further believe that the ability to differentially extract DNA from sperm and not
epithelial cells could reduce the cost of such testing, while increasing the quality, safety and speed of the testing process.

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Histology

The  most  commonly  used  technique  worldwide  for  the  preservation  of  cancer  and  other  tissues  for  long-term  storage  and  subsequent
pathology evaluation is to process them into formalin-fixed, paraffin-embedded (“FFPE”) samples. We believe that the quality and analysis of
FFPE tissues is highly problematic, and that PCT offers significant advantages over current processing methods, including standardization, speed,
biomolecule recovery, and safety.

Our customers include researchers at academic laboratories, government agencies, biotechnology companies, pharmaceutical companies and
other  life  science  institutions  in  the  United  States,  Europe,  and  in Asia.  Our  goal  is  to  continue  aggressive  market  penetration  in  these  target
groups.  We  also  believe  that  there  is  a  significant  opportunity  to  sell  and/or  lease  additional  Barocycler®  instrumentation  to  additional
laboratories at current customer institutions.

If  we  are  successful  in  commercializing  PCT  in  applications  beyond  our  current  focus  area  of  genomic,  proteomic,  lipidomic,  and  small
molecule sample preparation, and if we are successful in our attempts to attract additional capital, our potential customer base could expand to
include  hospitals,  reference  laboratories,  pharmaceutical  manufacturing  plants  and  other  sites  involved  in  each  specific  application.  If  we  are
successful in forensics, our potential customers could be forensic laboratories, military and other government agencies. If we are successful in
histology (extraction of biomolecules from FFPE tissues), our potential customers could be pharmaceutical companies, hospitals, and laboratories
focused on drug discovery or correlation of disease states.

Developments

We reported a number of accomplishments in 2017:

On December 20, 2017, we announced a significant software upgrade for our flagship Barocycler 2320EXTREME instrument.

On December 13, 2017 we announced the acquisition of all the assets of BaroFold Corp, and our immediate entry into the Biologics Contract
Research Services Sector.

On November 1, 2017, we announced that we had initiated an aggressive marketing and sales strategy expected to drive significant expansion in
China.

On  October  18,  2017,  we  announced  a  strategic  collaboration  with  Phasex  Corporation  addressing  broad  markets  for  stable,  water-soluble
nanoemulsions.

On October 10, 2017, we announced that our penetration into the European biopharma and high pressure markets was continuing to expand via
multiple scientific presentations in Germany, Poland, and Ireland.

On October 2, 2017, we announced that we were issued two patents on our widely-applicable, high pressure-based Ultra Shear Technology. PBI
believes that UST can be used to create or improve a broad range of medical, consumer, and industrial products through the preparation of high
quality nanoemulsions and “clean label” food.

On  September  18,  2017,  we  announced  that  the  Barocycler  2320EXTREME  was  named  a  finalist  in  the  prestigious  2017  R&D  100 Awards.
Known as the “Oscars of Innovation”, the R&D 100 Awards recognize the top 100 revolutionary technologies of the past year.

On June 5, 2017, we announced that Professor Ruedi Aebersold, a worldwide expert in proteomics and one of PBI’s most well-known clients,
received the prestigious Karger Medal for significant contributions to the development of new bioanalytical methods.

On  June  2,  2017,  we  announced  a  one-for-thirty  reverse  split  of  our  common  stock,  to  become  effective  on  June  5,  2017.  Please  see  the
Company’s second quarter Form 10Q for more details.

On April 10, 2017, we announced that Joseph Damasio, Jr. had joined the Company as its full-time Chief Financial Officer and Vice President of
Finance.

On  March  23,  2017,  we  announced  that  we  had  significantly  bolstered  our  marketing  and  sales  capabilities  by  contracting  with  EKG  Sales
Associates, a lead generation company and by hiring two of its planned four additional field sales directors.

On March 1, 2017, we announced that our Barocycler 2320EXTREME had been named the “Best New Instrument for Sample Preparation 2017”
by Corporate America News (“Corp America”) as part of the publication’s 2017 North American Excellence Awards.

On February 2, 2017, we announced that we had achieved CE Marking for the Barocycler 2320EXTREME, the Company’s recently released,
next-generation  PCT-based  sample  preparation  instrument.  CE  Marking  permits  PBI  to  begin  sales  of  the  Barocycler  2320EXT  to  the  31
countries of the European Economic Area.

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Liquidity

Management  has  developed  a  plan  to  continue  operations.  This  plan  includes  controlling  expenses,  streamlining  operations,  and  obtaining
capital  through  equity  and/or  debt  financing.  We  have  been  successful  in  raising  cash  through  debt  and  equity  offerings  in  the  past.  We  have
efforts in place to continue to raise cash through debt and equity offerings.

Although we have successfully completed equity financings and reduced expenses in the past, we cannot assure our investors that our plans to
address these matters in the future will be successful. Additional financing may not be available to us on a timely basis or on terms acceptable to
us, if at all. In the event we are unable to raise sufficient funds on terms acceptable to us, we may be required to:

● severely limit or cease our operations or otherwise reduce planned expenditures and forego other business opportunities, which could harm
our business. The accompanying financial statements do not include adjustments that may be required in the event of the disposal of assets
or the discontinuation of the business;

● obtain financing  with  terms  that  may  have  the  effect  of  diluting  or  adversely  affecting  the  holdings  or  the  rights  of  the  holders of  our

capital stock; or

● obtain funds through arrangements with future collaboration partners or others that may require us to relinquish rights to some or all of our

technologies or products.

Corporate Information

We were incorporated in the Commonwealth of Massachusetts in August 1978 as Boston Biomedica, Inc. In September 2004, we completed
the  sale  of  Boston  Biomedica’s  core  business  units  and  began  to  focus  exclusively  on  the  development  and  commercialization  of  the  PCT
platform. Following this change in business strategy, we changed our legal name from Boston Biomedica, Inc. to Pressure BioSciences, Inc. We
began  operations  as  PBI  in  February  2005,  research  and  development  activities  in April  2006,  early  marketing  and  selling  activities  of  our
Barocycler® instruments in late 2007, and active marketing and selling of our PCT-based instrument platform in 2012.

Available Information

Our Internet website address is http://www.pressurebiosciences.com. Through our website, we make available, free of charge, reports we file
with the Securities and Exchange Commission (“SEC”), which include, but are not limited to, our annual report on Form 10-K, quarterly reports
on Form 10-Q, current reports on Form 8-K and any and all amendments to such reports, as soon as reasonably practicable after we electronically
file such material with, or furnish it to, the SEC. These SEC reports can be also accessed through the investor relations section of our website. The
information found on our website is not part of this or any other report we file with or furnish to the SEC.

You  may  read  and  copy  any  materials  we  file  with  the  SEC  at  the  SEC’s  Public  Reference  Room  at  100  F  Street,  NE,  Washington,  DC
20549.  You  may  obtain  information  on  the  operation  of  the  Public  Reference  Room  by  calling  the  SEC  at  1-800-SEC-0330.  The  SEC  also
maintains an Internet website that contains reports, proxy and information statements and other information regarding Pressure BioSciences and
other issuers that file electronically with the SEC. The SEC’s Internet website address is http://www.sec.gov.

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Sample Preparation for Genomic, Proteomic, Lipidomic and Small Molecule Studies

The Market

Since February 2005, we have focused substantially all of our research and development and commercialization efforts on sample preparation
for  genomic,  proteomic,  lipidomic,  and  small  molecule  studies.  This  market  is  comprised  of  academic  and  government  research  institutions,
biotechnology  and  pharmaceutical  companies,  and  other  public  and  private  laboratories  that  are  engaged  in  studying  genomic,  proteomic  and
small molecule material within plant and animal cells and tissues. We elected to initially focus our resources in the market of genomic, proteomic
and small molecule sample preparation because we believe it is an area that:

● is a rapidly growing market;

● has a large and immediate need for better technology;

● is comprised mostly of research laboratories, which are subject to minimal governmental regulation;

● is the least technically challenging application for the development of our products;

● is compatible with our technical core competency; and

● we currently have strong patent protection.

We believe that our existing PCT and CP-based instrumentation and related consumable products fill an important and growing need in the
sample preparation market for the safe, rapid, versatile, reproducible and quality extraction of nucleic acids, proteins and small molecules from a
wide variety of plant and animal cells and tissues.

Biomarker Discovery - Mass Spectrometry

A biomarker is any substance (e.g., protein, DNA) that can be used as an indicator of the presence or absence of a particular disease-state or
condition,  and  to  measure  the  progression  and  effects  of  therapy.  Biomarkers  can  help  in  the  diagnosis,  prognosis,  therapy,  prevention,
surveillance, control, and cure of diseases and medical conditions.

A mass spectrometer is a laboratory instrument used in the analysis of biological samples, often focused on proteins, in life sciences research.
It  is  frequently  used  to  help  discover  biomarkers.  According  to  a  recently  published  market  report  by  Transparency  Market  Research,
“Spectrometry Market (Atomic, Molecular and Mass Spectrometry) - Global Scenario, Trends, Industry Analysis, Size, Share & Forecast 2011 –
2017,” the global spectrometry market was worth $10.2 billion in 2011 and is expected to reach $15.2 billion in 2017, growing at a compound
annual growth rate of 6.9% from 2011 to 2017. In the overall global market, the North American market is expected to maintain its lead position
in  terms  of  revenue  until  2017  and  is  expected  to  have  approximately  36.2%  of  the  market  revenue  share  in  2017,  followed  by  Europe.  We
believe PCT and CP-based products offer significant advantages in speed and quality compared with current techniques used in the preparation of
samples for mass spectrometry analysis.

Our  plan  is  to  focus  primarily  on  the  application  of  PCT-enhanced  protein  extraction  and  CP-based  digestion  for  the  mass  spectrometry
market and the advantages of PCT and CP in this market, and on the use of PCT and CP in biomarker discovery, soil and plant biology, counter
bio-terrorism and tissue pathology applications.

Forensics

The detection of DNA has become a part of the analysis of forensic samples by laboratories and criminal justice agencies worldwide in their
efforts to identify the perpetrators of violent crimes and missing persons. Scientists from the University of North Texas and Florida International
University have reported improvements in DNA yield from forensic samples (e.g., bone and hair) using PCT in the sample preparation process.
We believe that PCT may be capable of differentially extracting DNA from sperm cells and female epithelial cells in swabs collected from rape
victims and stored in rape kits. We also believe that there are many completed rape kits that remain untested for reasons such as cost, time and
quality of results. We further believe that the ability to differentially extract DNA from sperm and not epithelial cells could reduce the cost of
such testing, while increasing the quality, safety and speed of the testing process.

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Histology

The  most  commonly  used  technique  worldwide  for  the  preservation  of  cancer  and  other  tissues  for  subsequent  pathology  evaluation  is
formalin-fixation followed by paraffin-embedding, or FFPE. We believe that the quality and analysis of FFPE tissues is highly problematic, and
that PCT offers significant advantages over current processing methods, including standardization, speed, biomolecule recovery, and safety.

Sample Extraction Process

The  process  of  preparing  samples  for  genomic,  proteomic  and  small  molecule  studies  includes  a  crucial  step  called  sample  extraction  or
sample disruption. This is the process of extracting nucleic acid i.e., DNA and/or RNA, proteins or small molecules from the plant or animal cells
and  tissues  that  are  being  studied.  Sample  preparation  is  widely  regarded  as  a  significant  impediment  to  research  and  discovery  and  sample
extraction is generally regarded as one of the key parts of sample preparation. Our current commercialization efforts are based upon our belief that
pressure cycling technology provides a superior solution to sample extraction compared with other available technologies or procedures and can
thus significantly improve the quality of sample preparation, and thus the quality of the test result.

Company Products

We  believe  our  PCT  and  CP  products  allow  researchers  to  improve  scientific  research  studies  in  the  life  sciences  field.  Our  products  are
developed  with  the  expectation  of  meeting  or  exceeding  the  needs  of  research  scientists  while  enhancing  the  safety,  speed  and  quality  that  is
available to them with existing sample preparation methods.

Barocycler® Instrumentation

Our Barocycler® product line consists of laboratory instrumentation that subjects a sample to cycles of pressure from ambient (approximately

14.5 psi) to ultra-high levels (20,000 psi or greater) and then back to ambient, in a precisely controlled manner.

Our  instruments  (the  2320EXT,  the  Barozyme-HT48,  the  Barocycler®  NEP3229,  the  HUB440  and  the  HUB880)  use  cycles  of  high,
hydrostatic pressure to quickly and efficiently break up the cellular structures of a specimen to release proteins, nucleic acids, lipids and small
molecules from the specimen into our consumable processing tubes, referred to as our PULSE® Tubes and MicroTubes. Our instruments have
temperature  control  options  (on-board  heating  or  chilling  via  internal  heating  jacket  or  external  circulating  water-bath),  automatic  fill  and
dispensing valves, and an integrated micro-processor keypad or a laptop computer. The microprocessor or laptop computer are capable of saving
specific  PCT  protocols,  so  the  researcher  can  achieve  maximum  reproducibility  for  the  preparation  of  nucleic  acids,  proteins,  lipids,  or  small
molecules from various biological samples. Our Barocycler® instruments and our consumable products make  up  our  PCT  Sample  Preparation
System.

Barocycler®  2320EXTREME  -  The  Barocycler®  2320EXT  is  the  flagship  of  the  Company’s  Barocycler  line  of  PCT-based  instruments.  It
weighs approximately 80lbs, has a maximum pressure of 45,000 psi, and can process either up to 16 MicroTubes simultaneously or one PULSE®
Tube. The working temperature range is 4 – 95ºC and is controlled via an on-board electric heating jacket or external circulating water bath. All
tests  are  entered  and  recorded  on  a  touch  screen  interface.  Information  from  each  test  run  (pressure  profile,  cycle  number,  and  temperature)  is
recorded and can be stored on the instrument, on a USB drive, or networked into the user’s lab. Pressure profiles can be manipulated in a number
of  ways,  including  static  high  pressure  holds  and  pressure  ramp  programs.  The  Barocycler®  2320EXT  is  pneumatic,  and  requires  an  input  air
source of only 100psi to reach and cycle at high pressure.

- 9 -

 
 
 
 
 
 
 
 
 
 
 
 
The  Barocycler®  2320EXT  was  developed  to  support  the  PCT-HD/PCT-SWATH  application.  PCT-HD  enables  faster,  less  cumbersome  and
higher  quality  processing  of  biopsy  tissues.  With  homogenization,  extraction,  and  digestion  of  proteins  occurring  in  a  single  PCT  MicroTube
under high pressure, this protocol can yield analytical results in under four hours from the start of tissue processing. PCT-HD was developed by
our scientists and engineers in collaboration with Professor Ruedi Aebersold and Dr. Tiannan Guo of the Institute of Molecular Systems Biology,
ETH Zurich, and the University of Zurich, both in Zurich, Switzerland. Drs. Aebersold and Guo combined PCT-HD with SCIEX’s SWATH-Mass
Spectrometry – calling the resulting method “PCT-SWATH”.

Barocycler® NEP3229  –  The  Barocycler®  NEP3229  contains  two  units  –  a  user  interface  and  a  power  source  –  comprised  primarily  of  a  1.5
horsepower  motor  and  pump  assembly  (hydraulic).  Combined,  the  two  components  of  the  NEP3229  weigh  approximately  350  pounds.  The
Barocycler® NEP3229 is capable of processing up to three samples simultaneously using our specially designed, single-use PULSE® Tubes and
up to 48 samples simultaneously using our specially-designed MicroTubes.

Barozyme HT48 - The Barozyme HT48 is a high throughput, bench-top instrument designed for accelerated enzymatic digestion of proteins at
high  pressure. A  typical  protein  digestion  time  using  the  enzyme  trypsin  (a  common  yet  important  laboratory  procedure)  can  be  reduced  from
often  requiring  an  overnight  incubation  to  achieve  completion,  to  under  one  hour  when  the  digestion  procedure  is  carried  out  with  PCT.  The
Barozyme HT48 uses an air-pressure-to-liquid-pressure proprietary intensifier system, with a pressure amplification ratio  of  160:1,  to  reach  an
output pressure of 20,000 psi. The Barozyme HT48 is capable of processing up to 48 samples at a time in six single-use BaroFlex 8-well Strips in
the Barozyme Sample Carrier.

Barocycler® HUB440 –We believe the Barocycler® HUB440 is the first portable, ready to use, “plug-and-play” high pressure generator for the
laboratory bench. The Barocycler® HUB440 is capable of creating and controlling hydrostatic pressure from 500 psi to 58,000 psi. It is computer
controlled  and  runs  on  software  that  was  specially-written  by  us  in  LabVIEW  (software  from  National  Instruments  Corporation).  We  own  the
rights and have a license to use the specialty LabVIEW software. We believe that over the coming years, the Barocycler® HUB440 may become
the main instrument in our pressure-based instrument line.

Barocycler® HUB880 - The Barocycler® HUB880 is a compact, portable, bench-top, ultra-high pressure generator that uses an air pressure-to-
liquid pressure intensifier allowing the user to generate fluid pressure as high as 90,000 psi with input air pressure of just 126 psi. The HUB880
can be operated through a simple front panel or controlled using an optional external Data Acquisition and Control Module for dynamic pressure
control. We believe that the HUB880 will be well accepted by scientists that need to achieve super high pressure, such as those working in the
food safety and vaccine industries.

Ultra Shear Technology (UST) – UST is an emerging technology that utilizes intense fluid shear and instant heating achieved by specialized high
pressure  equipment  to  continuously  produce  commercially  sterile,  low  acid,  pumpable,  homogeneous  fluid  products.  This  requires  the
inactivation of bacteria, bacterial spores and enzymes. UST also achieves energetic cellular disruption and fluid homogenization. Consequently,
depending  on  operating  conditions,  nano-sized  emulsions  can  be  produced  that  have  been  shown  to  have  improved  shelf  stability,  flavor,  and
biological inactivation.

The  Company  received  two  patents  in  China  on  a  low  cost,  scalable  approach  for  production  level  product  manufacturing.  The  Company
believes  this  method  can  find  use  in  various  nanotechnology  applications  for  pharmaceutical  (e.g.,  drug  delivery),  biotechnology  (e.g.,  protein
recovery, biomolecule extraction), and food (e.g., shelf-stable “clean label” products) applications. We plan to design, develop, manufacture, and
market a lab-scale UST-based instrument that we can sell direct to the life sciences and other industries. We also plan to develop a pilot plant
scale UST-based instrument for demonstration in our expectation to license the technology to food companies worldwide.

The Shredder SG3 –The Shredder SG3 is a low shear mechanical homogenization system for use with tough, fibrous and other difficult-to-disrupt
tissues and organisms. The Shredder SG3 System uses a variety of Shredder PULSE® Tubes to directly and rapidly grind a biological sample
which, when combined with selected buffers, can provide effective extraction of proteins, DNA, RNA, lipids and small molecules from tissues
and  organisms.  The  Shredder  SG3  is  also  used  to  isolate  intact  and  functional  mitochondria  from  tissues.  The  Shredder  SG3  features  a  three
position  force  setting  lever,  which  enables  the  operator  to  select  and  apply  reproducible  force  to  the  sample  during  the  shredding  process  and
eliminates the need for the operator to exert force for long periods when processing one or more samples.

Barocycler® Consumable Products

PCT  MicroTubes  – PCT  MicroTubes  are  made  from  a  unique  fluoropolymer,  fluorinated  ethylene  propylene  (FEP).  FEP  is  highly  inert  and
retains  its  integrity  within  an  extremely  wide  temperature  range  (-200oC  to  +100oC).  MicroTubes  hold  a  maximum  total  volume  of  150
microliters. PCT MicroTubes must be used with either PCT-MicroCaps or PCT-MicroPestles.

PCT-MicroCaps  – PCT  MicroCaps  are  made  from  polytetraflouroethylene  (PTFE).  The  PCT  MicroCaps  are  available  in  three  sizes  to
accommodate total sample volume: 50, 100 and 150uL. 50uL MicroCaps are used with samples ≤50uL, 100uL MicroCaps are used with samples
between 50-100uL, and 150uL MicroCaps are used with samples between 100-150uL.

- 10 -

 
 
 
 
 
 
 
 
 
 
 
 
 
PCT-Micro Pestle - PCT μPestles are made from Polytetrafluoroethylene (PTFE), a synthetic fluoropolymer of tetrafluoroethylene, also known as
Teflon  (by  DuPont  Co).  PTFE  is  practically  inert;  the  only  chemicals  known  to  affect  it  are  certain  alkali  metals  and  most  highly-reactive
fluorinating agents. PCT μPestles, in conjunction with PCT MicroTubes, are designed to enhance the extraction of proteins, lipids, DNA, RNA
and small molecules from minute amounts (0.5 – 3.0 mg) of solid tissue in extraction reagent volumes as low as 20-30 μL. PCT MicroTubes and
PCT μPestles use PCT to effectively disrupt soft tissues and lyse their cells. As a result, the tissue sample trapped between the MicroTube end and
the μPestles tip is crushed on every pressure cycle. This mechanical action, combined with the extraction ability of the buffer under high pressure,
results in highly effective tissue homogenization and extraction.

PCT μPestles and PCT MicroTubes, together with a PBI Barocycler®, comprise the PCT Micro-Pestle System, which provides a fast, safe, and
efficient means of extraction from extremely small amounts of solid samples such as soft animal tissues or biopsies. The PCT μPestle System can
be used in any PBI Barocycler®.

BaroFlex 8-well Processing Strips - BaroFlex 8-well Strips are used in the Barozyme HT48 (for pressure-enhanced enzymatic digestion at 20,000
psi). BaroFlex 8-well Strips are made of special high density polyethylene (HDPE) and hold up to 140µl when capped with the BaroFlex Cap
Strips or Mats. BaroFlex 8-Cap Strips and BaroFlex 24-Cap Mats are made of silicone. These single-use caps are designed to seal BaroFlex 8-
well Strips tightly and to prevent fluid exchange between the sample and the Barozyme chamber fluid during pressure cycling. The silicone caps
are available as strips of eight, or mats of 24 caps.

We believe our development of these various consumable products has helped, and will continue to help, drive the adoption of PCT within the
life sciences market.

Company Services

Government Grants and Contracts

We view federal agency grants to be an important part of our business plan. These types of grants allow us to bill the federal agency for work
that  we  are  planning  to  perform  as  part  of  the  development  and  commercialization  of  our  technology.  We  generally  start  by  submitting  initial
grant  requests  that  are  in  response  to  requests  for  proposals  (“RFPs”)  from  the  federal  government  through  their  Small  Business  Innovation
Research (“SBIR”)  program.  Initial  (“SBIR  Phase  I”)  grants  are  meant  to  fund  approved  research  projects  for  six  months,  and  generally  have
budgets of approximately $100,000 to $150,000. Because our work in SBIR Phase I grants has been successful, we have applied, and may in the
future apply for larger National Institutes of Health (“NIH”) SBIR Phase II grants. Such larger grants are typically for a two-year period and can
offer  as  much  as  $1,000,000  to  support  significant  research  projects  in  areas  we  would  otherwise  expect  to  support  with  internal  funds  should
SBIR Phase II grants not be awarded. To date, we have been awarded five NIH SBIR Phase I grants and three SBIR Phase II grants. The data on
three of the NIH SBIR Phase I grants were the basis for the submission, and subsequent award. Of the three NIH SBIR Phase II grants awarded to
us: one was in the approximate amount of $845,000 in August 2008, the second was in the approximate amount of $850,000 in September 2011,
and the third award was in the approximate amount of $1,020,000 awarded in November 2014. All five of the NIH SBIR Phase I grants and the
August  2008  and  September  2011,  NIH  SBIR  Phase  II  grants  have  been  completed.  We  received  an  extension  on  the  SBIR  Phase  II  grant,
awarded in November 2014, to utilize unused funds until November 30, 2018.

The 2008 SBIR Phase II grant (2R44GM079059) was awarded to us by the NIH for work in the area of using PCT to extract proteins, sub-
cellular molecular complexes, and organelles, with the expectation that these studies might ultimately lead to the release of a new, commercially
available  PCT-based  system,  with  validated  protocols,  end-user  kits,  and  other  consumables  intended  for  the  extraction  of  clinically  important
protein biomarkers, sub-cellular molecular complexes, and organelles from human and animal tissues. The 2011 SBIR II contract (W81XWH-10-
C-0-175)  was  awarded  to  us  by  the  U.S. Army  for  the  development  of  a  universal  method  for  the  inactivation,  extraction,  and  enrichment  of
pathogens in diagnostic samples, including arthropod hosts of military importance. The work covered by this grant was significant in helping us
develop  the  Barozyme  HT48  High  Throughput  System.  The  2014  SBIR  Phase  II  grant  (2R44HG007136)  was  awarded  to  us  by  the  National
Human  Genome  Research  Institute  of  the  NIH.  Entitled  “High  Pressure  Sample  Preparation  Instrumentation  for  DNA  Sequencing”,  this  grant
allowed  us  to  develop  the  Barocycler  HUB880,  an  automated,  high-throughput,  high  pressure  system  (instrument  and  consumables),  to  enable
significantly better control of DNA fragmentation - a critical step in the preparation of samples for Next Generation Sequencing platforms. This
system was based on significant technological advancements over the classic hydrodynamic DNA shearing approach that has been successfully
and widely used in the field of DNA sequencing for many years.

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Extended Service Contracts

We offer extended service contracts on our laboratory instrumentation to all of our customers. These service contracts allow a customer who
purchases  a  Barocycler®  instrument  to  receive  on-site  scheduled  preventative  maintenance,  on-site  repair  and  replacement  of  all  worn  or
defective component parts, and telephone support, all at no incremental cost for the life of the service contract. We offer one-year and four-year
extended service contracts to customers who purchase Barocycler® instruments.

Other Fields of Use and Applications for PCT

Our research and development efforts have shown that, in addition to genomic, proteomic, lipidomic, and small molecule sample preparation,
PCT  is  potentially  beneficial  in  a  number  of  other  areas  of  the  life  sciences,  including  pathogen  inactivation,  protein  purification,  control  of
chemical (particularly enzymatic) reactions, and immunodiagnostics. Other applications in the sample preparation market include forensics and
histology, as discussed above. Our pursuit of these markets, however, depends on a number of factors, including our success in commercializing
PCT in the area of sample preparation, our judgment regarding the investment required to be successful in these areas, the value of these markets
to PBI, and the availability of sufficient financial resources. Below is a brief explanation of each of these additional potential applications and a
short description of why we believe PCT can be used to improve scientific studies in these areas.

Pathogen Inactivation

Biological products intended for human use, such as blood, vaccines and drugs, are put through rigorous processing protocols in an effort to
minimize  the  potential  of  that  product  to  transmit  disease.  These  protocols  may  include  methods  to  remove  infectious  materials  such  as  pre-
processing  testing,  filtration  or  chromatography,  or  methods  to  inactivate  infectious  agents  that  are  not  captured  in  the  removal  steps  such  as
pasteurization,  irradiation  and  solvent  detergent  inactivation.  Notwithstanding  current  diligence  in  both  the  removal  and  inactivation  steps,
significant concern remains that some pathogens (e.g., bacteria and viruses) capable of transmitting infection to recipients may not be removed or
inactivated with current procedures. In addition, some removal and inactivation methods may not be useful because of cost, safety, ease-of-use or
other practical concerns. To that end, we believe that a new inactivation method is needed that can safely, rapidly and inexpensively inactivate
pathogens  in  blood,  vaccines  and  drugs  without  the  need  for  chemical  or  other  potentially  toxic  additives.  We  believe  we  have  successfully
generated proof-of-concept that PCT can satisfy this need. We believe that compared with current procedures, a process that uses PCT has the
potential to increase safety and yield, lower cost and decrease the potential side effects of current methods. We have been issued U.S. patents for
this PCT-dependent inactivation technology.

Protein Purification

Many vaccines and drugs are comprised of proteins. These proteins need to be purified from complex mixtures as part of the manufacturing
process. Current purification techniques often result in the loss of a significant amount of the protein. Therefore, any method that could increase
the amount of protein being recovered in the purification step, could subsequently lead to a reduction in cost to the manufacturer. We believe we
have successfully generated proof-of-concept that PCT can satisfy this need. We believe that compared with current purification procedures, a
process that uses PCT has the potential to increase  protein  recovery,  increase  the  quality  of  the  product,  and  lower  production  costs.  We  have
been issued U.S. patents in this area.

- 12 -

 
 
 
 
 
 
 
 
 
 
Control of Chemical (Particularly Enzymatic) Reactions

Chemical reactions encompass many important interactions in nature. Methods used to control chemical reactions could have a positive effect
on the quality, speed, and overall result of the reaction. The control and detection of chemical reactions is particularly useful in the biotechnology
field  for  synthesizing  and  characterizing  such  molecules  as  nucleic  acids  and  polypeptides.  We  believe  that  PCT  offers  distinct  advantages  in
controlling chemical reactions over current methods, since PCT can provide precise, automated control over the timing and synchronization of
chemical reactions, particularly enzymatic reactions. We have been issued U.S patents in this area.

Immunodiagnostics

Many tests used in the clinical laboratory today are based on the formation of a complex between two proteins, such as an antigen and an
antibody. Such “immunodiagnostic” methods are used for the detection of infectious agents such as the human immunodeficiency virus (“ HIV”),
hepatitis viruses, West Nile virus, and others, as well as for endocrine, drug testing and cancer diagnostics. We have generated proof-of-concept
that  PCT  may  be  used  to  control  biomolecular  interactions  between  proteins,  such  as  antigens  and  antibodies.  We  believe  this  capability  may
provide  a  greater  degree  of  sensitivity  and  quantitative  accuracy  in  immunodiagnostic  testing  than  that  offered  by  methods  that  are  available
today. We have been issued U.S. patents in this area.

Acquisition of BaroFold’s PreEMT™ high-pressure protein refolding technology in December 2017

BaroFold’s assets have significantly increased PBI’s intellectual property portfolio in high-pressure technologies with the addition of eight
issued  and  several  pending  patents.  These  patents  give  PBI  the  ability  to  operate  in  several  important  areas  for  biologics  research  and
manufacturing: protein folding, re-folding and disaggregation. The patents also provide PBI the right to grant licenses to third parties to practice
the PreEMT and other technologies in both research laboratories and in biopharmaceutical manufacturing.

Biopharmaceutical products are typically large-molecule proteins produced via complex biological manufacturing processes that can lead to
undesirable protein misfolding and aggregation. Misfolded or aggregated proteins typically lack therapeutic activity and can present health risks
to  patients,  requiring  robust  remediation  within  pharmaceutical  manufacturing  processes.  The  PreEMT  technology  improves  the  quality  of
manufacturing,  decreases  manufacturing  costs  (as  much  as  $2-10M/year  per  commercial  biologic  drug),  and  facilitates  achievement  of  proper
activity from difficult-to-manufacture proteins.

Customers

Our customers include researchers at academic laboratories, government agencies, biotechnology companies, pharmaceutical firms, and other
life science institutions in North, Central, and South America; Europe; and Asia. Our goal is to continue aggressive market penetration to target
groups  in  these  geographical  areas.  We  also  believe  that  there  is  a  significant  opportunity  to  sell  and/or  lease  additional  Barocycler®
instrumentation to additional laboratories at current customer institutions.

If  we  are  successful  in  commercializing  PCT  in  applications  beyond  our  current  focus  area  of  genomic,  proteomic,  lipidomic,  and  small
molecule sample preparation, and if we are successful in our attempts to attract additional capital, our potential customer base could expand to
include  hospitals,  reference  laboratories,  pharmaceutical  manufacturing  plants,  and  other  sites  involved  in  each  specific  application.  If  we  are
successful in forensics, our potential customers could be forensic laboratories, military and other government agencies. If we are successful in
histology (extraction of biomolecules from FFPE tissues), our potential customers could be pharmaceutical companies, hospitals, and laboratories
focused on drug discovery or correlation of disease states.

Competition

We compete with companies that have existing technologies for the extraction of nucleic acids, proteins, lipids, and small molecules from
cells  and  tissues,  including  methods  such  as  mortar  and  pestle  grinding,  sonication,  rotor-stator  homogenization,  French  Press,  bead  beating,
freezer milling, enzymatic digestion, and chemical dissolution. We believe that there are a number of significant issues related to the use of these
methods, including: complexity, sample containment, cross-contamination, shearing of biomolecules of interest, limited applicability to different
sample  types,  ease-of-use,  reproducibility,  and  cost.  We  believe  that  our  PCT  Sample  Preparation  System  offers  a  number  of  significant
advantages over these methods, including:

●

●

●

●

labor reduction

temperature control

precision

reproducibility

● versatility

● efficiency

● simplicity

● safety

To  be  competitive  in  the  industry,  we  believe  we  must  be  able  to  clearly  and  conclusively  demonstrate  to  potential  customers  that  our
products provide these improved performance capabilities. We strongly believe that our PCT Sample Preparation System is a novel and enabling
system for genomic, proteomic, and small molecule sample preparation. As such, many users of current manual techniques will need to be willing
to challenge their existing methods of sample preparation and invest time to evaluate a method that could change their overall workflow in the
sample preparation process, prior to adopting our technology.

- 13 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Further,  we  are  aware  that  the  cost  of  the  PCT  Sample  Preparation  System  may  be  greater  than  the  cost  of  many  of  the  other  methods
currently  employed.  Consequently,  we  are  focusing  our  sales  efforts  on  those  product  attributes  that  we  believe  will  be  most  important  and
appealing to potential customers; namely versatility, reproducibility, quality, and safety.

Manufacturing and Supply

CBM Industries (Taunton, MA) is the manufacturer of the Barocycler® 2320EXT. CBM is ISO 13485:2003 and 9001:2008 Certified. CBM
provides  us  with  precision  manufacturing  services  that  include  management  support  services  to  meet  our  specific  application  and  operational
requirements. Among the services provided by CBM to us are:

● CNC Machining

● Contract Assembly & Kitting

● Component and Subassembly Design

● Inventory Management

● ISO certification

At this time, we believe that outsourcing the manufacturing of our Barocycler® 2320EXT to CBM is the most cost-effective method for us to
obtain ISO Certified, CE and CSA Marked instruments. CBM’s close proximity to our South Easton, MA facility is a significant asset enabling
interactions between our Engineering, R&D, and Manufacturing groups and their counterparts at CBM. CBM was instrumental in helping PBI
achieve CE Marking on our Barocycler 2320EXT, as announced on February 2, 2017.

Although  we  currently  manufacture  and  assemble  the  Barozyme  HT48,  Barocycler®  HUB440,  the  SHREDDER  SG3,  and  most  of  our
consumables at our South Easton, MA facility, we plan to take advantage of the established relationship with CBM and transfer manufacturing of
the entire Barocycler® product line, future instrument, and other products to CBM.

The  Barocycler®  NEP3229,  launched  in  2008,  and  manufactured  by  the  BIT  Group,  will  be  phased  out  over  the  next  several  years  and

replaced by the new state-of-the-art Barocycler® HUB and Barozyme HT product lines.

Research and Development

Our research and development activities are split into two functional areas: Applications Development and Engineering.

1. Applications Development R&D: Our highly educated and trained staff has years of experience in molecular and cellular biology, virology,
and proteomics. Our team of scientists focuses on the development and continued improvement of the PCT Sample Preparation System and
on PCT-dependent genomic, proteomic, lipidomic, and small molecule sample preparation applications. Dr. Alexander  Lazarev, our vice
president  of Applications  Research  &  Development,  meets  regularly  with  our  sales,  marketing,  and  engineering staff  to  discuss  market
needs and trends. Our applications research and development team is responsible for the technical review of all scientific collaborations,
for the support of our marketing and sales departments through the generation of internal data in a number of areas of market interest, and
in the development of commercially-viable PCT-dependent products.

2. Engineering R&D: Our  engineering  research  and  development  team  is  focused  on  the  design  and  development  of  new  and  improved
instrumentation and  consumable  products  to  support  the  commercialization  of  PCT.  Our  engineering  department  is  led  by  Dr.  Edmund
Ting, our senior vice president of Engineering. The primary focus of our engineering group is to develop and continually improve our line
of PCT-based instruments and consumables, ensure seamless production processes, help perform installations and field service, and work
with our application scientists to enhance our PCT-based systems for the mass spectrometry and other markets.

- 14 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Collaboration Program

Our  Collaboration  Program  is  an  important  element  of  our  business  strategy.  Initiating  a  collaboration  with  a  researcher  involves  the
installation of a Barocycler® instrument for an agreed upon period of time of approximately three to twelve months, a financial commitment that
is  beneficial  to  both  the  collaborator  and  PBI,  and  the  execution  of  an  agreed  upon  work  plan.  Our  primary  objectives  for  entering  into  a
collaboration agreement include:

● the development of a new application for PCT and CP in sample preparation;

● the advancement and validation of our understanding of PCT and CP within an area of life sciences in which we already offer products;

● the demonstration of the effectiveness of PCT and CP by specific research scientists, particularly Key Opinion Leaders (“KOLs”), who we

believe can have a positive impact on market acceptance of PCT; and

● the expectation of peer-reviewed publications and/or presentations at scientific meetings by a third party, especially a KOL, on the merits

of PCT and CP.

Since  we  initiated  our  collaboration  program,  third  party  researchers  have  cited  the  use  of  our  PCT  platform  in  multiple  publications  and
presentations. We believe that this program has provided and continues to provide us with independent and objective data about PCT from well-
respected  laboratories  in  the  United  States  and  throughout  the  rest  of  the  world.  We  believe  this  program  has  been  responsible  for  the  sale  of
multiple Barocycler instruments over the past few years, and will continue to help to increase the sales of instrument systems in the future.

Product Pipeline

The following instruments are in our research and development pipeline:

● Barocycler® FFPE Protein Extraction Instrument System - A PCT-based system offering the enhanced extraction of proteins from FFPE
samples  using  a  modified  Barocycler®  instrument  that  combines  the  advantages  of  pressure  cycling,  high  temperature,  and certain
reagents.

● XstreamPCT™ HPLC  Digestion  Module  -  For  automated,  in-line,  on-demand  PCT-enhanced  protein  digestion;  the  first  module  in  our

PCT-based HPLC platform.

Sales and Marketing

Our  marketing  and  sales  function  is  led  by  Dr.  Nathan  Lawrence,  our  vice  president  of  Marketing  and  Sales.  Dr.  Lawrence  oversees  and
directs marketing and sales activities such as trade show attendance and sponsorship, on-line advertising, website maintenance and improvement,
search engine optimization, creation and dissemination of a PCT newsletter, market research initiatives, the arrangement of on-location seminars,
lectures,  and  demonstrations  of  PCT  capabilities,  and  the  supervision  of  our  one-person  sales  force.  Dr.  Lawrence  is  also  responsible  for  the
overall  coordination  of  our  collaboration  programs,  from  initial  set-up,  research  plan  design,  and  training,  service,  and  data  analysis.  Some  of
these  responsibilities  are  shared  with  other  departments  such  as  Research  and  Development,  but  marketing  and  sales  drives  the  collaborative
process. Dr. Lawrence is also responsible for the continued coordination and support of our foreign distribution partners.

- 15 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Our  sales  and  marketing  efforts  are  centered  on  using  the  independent  data  developed  and  disseminated  by  our  collaboration  partners  to  help
drive the installed base of our PCT Sample Preparation System. The development of scientific data by our partners and our internal researchers
provides our sales and marketing staff with additional tools that are essential in selling a paradigm-shifting, new technology such as PCT.

Sales

Direct US Sales Force

Our domestic sales force currently consists of one sales director and three field salespersons. We expect to hire additional sales and marketing
personnel throughout 2018, with a goal that our sales and marketing department will have a minimum of six staff focused on sales and two on
marketing by the end of 2018.

Marketing Strategy

We recognize that our enabling pressure cycling technology (PCT) is novel. Consequently, the power of PCT is not yet generally known by
researchers.  Our  first  goal  is  to  greatly  broaden  the  awareness  of  PCT  and  its  applications  among  scientists  and  to  ensure  they  know  that  this
technology  exists  through  our  Barocycler®  family  of  high-pressure  instruments  and  requisite  consumables.  To  accomplish  this  expansion  of
knowledge  about  PCT  and  the  subsequent  adoption  of  our  PCT-based  products,  we  have  developed  and  are  implementing  a  multi-faceted
approach to marketing the PCT platform.

Key Opinion Leaders and Publications

To initially reach scientists, we have established collaborations with key opinion leaders (KOL) who recognized early the potential for PCT
and  went  on  to  report  their  discoveries  in  peer  reviewed  journals. Among  the  KOLs  working  with  us  is  Dr.  Ruedi Aebersold  (Head  of  the
Department of Biology, ETH, Zurich). Dr. Aebersold, a pioneer in proteomics, worked with our scientists and engineers to develop PCT-SWATH
(aka PCT-HD), a superior method for the extraction and preparation of proteins for the downstream analysis by mass spectrometry. Other KOLs
include  Dr.  Jennifer  van  Eyk  (Director  of Advanced  Clinical  Biosystems  Institute  in  the  Department  of  Biomedical  Sciences Cedar  Sinai,  Los
Angeles, CA) and Dr. Wayne Hubble (Jules Stein Professor at the University of California, LA). Dr. van Eyk is a recognized expert in the causes
of  heart  disease  and  is  using  PCT  in  her  attempt  to  discover  cardiac  disease  biomarkers.  Dr.  Hubble,  a  member  of  the  National Academy  of
Science,  is  a  leader  in  the  field  of  electron  paramagnetic  resonance  (EPR).  He  uses  PCT  in  his  studies  of  protein-protein  interactions,  so  very
important  in  the  discovery  of  drugs  and  drug  design.  The  publications  and  presentations  of  these  and  other  world  class  scientists  have  been
invaluable in gaining initial entry of PCT in several areas of research. In addition to publications by our KOLs, there are also many peer reviewed
publications from dozens of other scientists discussing the advantages of the PCT platform in bio-molecule sample preparation. To this end, we do
all we can to disseminate the work of these scientists in an effort to increase the exposure of PCT to the worldwide research community.

Broadcasting PCT and Our Products

1. We attend, exhibit, and present at top scientific meetings such as the American Society of Mass Spectrometry (ASMS) and both the US
and International meetings of the Human Proteome Organization (HUPO). These meetings are an opportunity to present our technology
and to showcase our products to scientists who require sample preparation in their research studies.

2. Routine and timely “blast” emails to scientists in our database. Topics include new PCT-related publications, announcements  of meetings,
product  advertisements,  and  a  monthly  newsletter.  The  database  we  use  is  proprietary,  as  it  has  been  built  from  attending  scientific
meetings and searching the internet for relevant publications and contact information.

3. We manage our database with SalesForce, a state-of-the-art Customer Relationship Management (CRM) system. Through SalesForce, we
employ the marketing automation software Pardot to manage our email blasts. Pardot enables us to assess open rates, levels of interest, and
to create automatic and constant contact with potential clients.

- 16 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
4. We  use  social  media  platforms  like  LinkedIn,  Twitter  and  Facebook  to  broadcast  publications,  webinars,  our  presence  at  scientific

meetings, and press releases. Social media enables us to easily reach scientists world-wide.

5. We significantly upgraded our website. The upgraded website contains a state-of-the art search engine that enables researchers to rapidly

find PCT-related publications and products.

6. The website contains videos of our products. In 2016, we contracted with BioCompare to produce a high quality video showing PCT-HD

and the uses of our Barocycler® 2320EXT and the MicroTube System.

7. Our scientists  regularly  present  their  findings  and  discuss  our  products  at  scientific  sessions  at  regional,  national,  and  international

scientific conferences, and at corporate, government, and academic laboratories.

8.

In addition to electronic advertising, we have used and will continue to use print media to showcase our products.

In 2018, we plan to expand our Marketing team to support these and additional initiatives.

Foreign Distributor Network

Exclusive Agreements

Currently, we have distribution arrangements covering China, Poland, 24 countries in Europe, and Japan.

In May of 2014, we entered into a three-year distribution agreement with Powertech Technology Co, Ltd., of China, pursuant to which we were
granted Powertech Technology exclusive distribution rights to all of our products in China. We expect this agreement will be extended during
2018.

In  February  2016,  we  entered  into  a  three-year  distribution  agreement  with bioanalytic  of  Poland,  pursuant  to  which  PBI  granted bioanalytic
exclusive distribution rights to all of our products in Poland.

In September of 2016, we entered into a three-year distribution agreement with Vita Co. of Japan, pursuant to which we were granted Vita Co.
exclusive distribution rights to all of our products in Japan.

In September of 2016, we entered into a distribution agreement with I&L GmbH, of Germany pursuant, to which were granted I&L, exclusive
distribution  rights  to  all  of  our  products  until  March  30,  2018  in  the  countries  designated  as  Western  Europe  (Andorra,  Austria,  Belgium,
Denmark,  Finland,  France,  Germany,  Gibraltar,  Greece,  Iceland,  Italy,  Ireland,  Liechtenstein,  Luxembourg,  Malta,  Monaco,  Norway,
Netherlands, Portugal, San Marino, Spain, Sweden, Switzerland, and the United Kingdom). We recently renewed the agreement to commence on
March 31, 2018 and end on March 31, 2019.

Non-Exclusive and Other Distribution Agreements

In November 2011, we entered into a distributor agreement with OROBOROS Instruments Corp. (“OROBOROS”) of Austria pursuant to which
we were granted OROBOROS non-exclusive world-wide distribution rights to our Shredder SG3 System and related products.

In June 2013, CS and PBI signed an expanded Distribution Agreement that made us the exclusive distributor of CS products throughout all of the
Americas until 2019.

In January 2016, SCIEX, a global leader in life science analytical technologies, announced an exclusive two-year co-marketing agreement with
PBI.  In  their  press  release,  SCIEX  stated  that  the  relationship  with  us  will  uniquely  position  SCIEX  to  address  a  major  challenge  in  complex
sample  preparation  by  marketing  a  complete  solution  to  increase  the  depth,  breadth,  and  reproducibility  of  protein  extraction,  digestion,  and
quantitation  in  all  tissue  types,  including  challenging  samples  like  tumors.  Under  the  agreement,  PBI  and  SCIEX  will  promote  PCT  Sample
Preparation Systems such as PCT-HD with SWATH® Acquisition-based next generation proteomics, TripleTOF® Systems, QTRAP® Systems,
and Triple Quad Systems. This focus on improved sample preparation, a crucial step performed in research laboratories worldwide, will enable
scientists to extract more proteins reproducibly from complex sample types, potentially yielding superior biological insights and discoveries. We
believe this agreement may be extended at some point during 2018, or that we might enter into an exclusive or a non-exclusive agreement with
one or more of the other five companies that sell mass spectrometers.

- 17 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Intellectual Property

We believe that protection of our patents and other intellectual property is essential to our business. Subject to the availability of sufficient
financial resources, our practice is to file patent applications to protect technology, inventions, and improvements to inventions that are important
to  our  business  development.  We  also  rely  on  trade  secrets,  know-how,  and  technological  innovations  to  develop  and  maintain  our  potential
competitive position.

To date, we have been granted 15 United States and foreign patents related to our PCT technology platform, and two additional patents in

China related to our Ultra Shear Technology. We also received eight patents with our purchase of the assets of BaroFold in December 2017.

Our issued patents expire between 2018 and 2027. Our failure to obtain and maintain adequate patent protection may adversely affect our
ability  to  enter  into,  or  affect  the  terms  of,  any  arrangement  for  the  marketing  or  sale  of  any  of  our  PCT  products.  It  may  also  allow  our
competitors to duplicate our products without our permission and without compensation.

License Agreements Relating to Pressure Cycling Technology

BioMolecular Assays, Inc.

In 1996, we acquired our initial equity interest in BioSeq, Inc., which at the time was developing our original pressure cycling technology.
BioSeq,  Inc.  acquired  its  pressure  cycling  technology  from  BioMolecular  Assays,  Inc.  under  a  technology  transfer  and  patent  assignment
agreement. In 1998, we purchased all of the remaining outstanding capital stock of BioSeq, Inc., and at such time, the technology transfer and
patent assignment agreement was amended to require us to pay BioMolecular Assays, Inc., a 5% royalty on our sales of products or services that
incorporate or utilize the original pressure cycling technology that BioSeq, Inc. acquired from BioMolecular Assays, Inc. We are also required to
pay  BioMolecular Assays,  Inc.  5%  of  the  proceeds  from  any  sale,  transfer  or  license  of  all  or  any  portion  of  the  original  pressure  cycling
technology. These payment obligations terminated March 7, 2016.

In  connection  with  our  acquisition  of  BioSeq,  Inc.,  we  licensed  certain  limited  rights  to  the  original  pressure  cycling  technology  back  to
BioMolecular Assays, Inc. This license is non-exclusive and limits the use of the original pressure cycling technology by BioMolecular Assays,
Inc.  solely  for  molecular  applications  in  scientific  research  and  development  and  in  scientific  plant  research  and  development.  BioMolecular
Assays, Inc. is required to pay us a royalty equal to 20% of any license or other fees and royalties, but not including research support and similar
payments, it receives in connection with any sale, assignment, license or other transfer of any rights granted to BioMolecular Assays, Inc. under
the license. BioMolecular Assays, Inc. was required to pay us these royalties until the expiration in March 2016 of the patents held by BioSeq,
Inc. since 1998. We have not received any royalty payments from BioMolecular Assays, Inc. under this license.

Battelle Memorial Institute

In  December  2008,  we  entered  into  an  exclusive  patent  license  agreement  with  the  Battelle  Memorial  Institute  (“Battelle”).  The  licensed
technology  is  the  subject  of  a  patent  application  filed  by  Battelle  in  2008  and  relates  to  a  method  and  a  system  for  improving  the  analysis  of
protein samples, including through an automated system utilizing pressure and a pre-selected agent to obtain a digested sample in a significantly
shorter  period  of  time  than  current  methods,  while  maintaining  the  integrity  of  the  sample  throughout  the  preparatory  process.  In  addition  to
royalty payments on net sales on “licensed products,” we are obligated to make minimum royalty payments for each year that we retain the rights
outlined in the patent license agreement and we are required to have our first commercial sale of the licensed products within one year following
the issuance of the patent covered by the licensed technology. After re-negotiating the terms of the contract in 2013, the minimum annual royalty
was $1,200 in 2014 and $2,000 in 2015; the minimum royalties are $3,000 in 2016, $4,000 in 2017 and $5,000 in 2018 and each calendar year
thereafter during the term of the agreement.

- 18 -

 
 
 
 
 
 
 
 
 
 
 
 
Regulation

Many of our activities are subject to regulation by governmental authorities within the United States and similar bodies outside of the United
States.  The  regulatory  authorities  may  govern  the  collection,  testing,  manufacturing,  safety,  efficacy,  labeling,  storage,  record  keeping,
transportation, approval, advertising, and promotion of our products, as well as the training of our employees.

Currently,  all  of  our  commercialization  efforts  are  focused  in  the  area  of  genomic,  proteomic,  lipidomic,  and  small  molecule  sample
preparation.  We  do  not  believe  that  our  current  Barocycler®  products  used  in  sample  preparation  are  considered  “medical  devices”  under  the
United States Food, Drug and Cosmetic Act (the “FDA Act”) and we do not believe that we are subject to the law’s general control provisions that
include requirements for registration, listing of devices, quality regulations, labeling and prohibitions against misbranding and adulteration. We
also  do  not  believe  that  we  are  subject  to  regulatory  inspection  and  scrutiny.  If,  however,  we  are  successful  in  commercializing  PCT  in
applications beyond our current focus area of genomic, proteomic, lipidomic, and small molecule sample preparation, such as protein purification,
pathogen inactivation and immunodiagnostics, our products may be considered “medical devices” under the FDA Act, at which point we would
be subject to the law’s general control provisions and regulation by the FDA that include requirements for registration listing of devices, quality
regulations,  labeling,  and  prohibitions  against  misbranding  and  adulteration.  The  process  of  obtaining  approval  to  market  these  devices  in  the
other potential applications of PCT would be costly and time consuming and could possibly prohibit us from pursuing such markets.

Some  of  our  devices  may  also  become  subject  to  the  European  Pressure  Equipment  Directive,  which  requires  certain  pressure  equipment
meet certain quality and safety standards. We do not believe that we are currently subject to this directive because our Barocycler® instruments
are  below  the  threshold  documented  in  the  text  of  the  directive.  If  our  interpretation  were  to  be  challenged,  we  could  incur  significant  costs
defending the challenge, and we could face production and selling delays, all of which could harm our business.

We  self-certified  that  our  Barocycler®  instrumentation  was  electromagnetically  compatible,  or  “CE”  compliant,  which  means  that  our
Barocycler®  instruments  meet  the  essential  requirements  of  the  relevant  European  health,  safety  and  environmental  protection  legislation.  In
order to maintain our CE Marking, a requirement to sell equipment in many countries of the European Union, we are obligated to uphold certain
safety and quality standards. Due to outsourcing manufacturing to CBM, an ISO certified contract manufacturer, we believe compliance with CE
and other required marks and certifications is well controlled.

Employees

At December 31, 2017, we had fourteen (14) full-time employees and five (5) part-time employees. All employees enter into confidentiality
agreements intended to protect our proprietary information. We believe that our relations with our employees are good. None of our employees
are represented by a labor union. Our performance depends on our ability to attract and retain qualified professional, scientific and technical staff.
The  level  of  competition  among  employers  for  skilled  personnel  is  high.  Subject  to  our  limited  financial  resources,  we  attempt  to  maintain
employee benefit plans to enhance employee morale, professional commitment and work productivity and provide an incentive for employees to
remain with us.

- 19 -

 
 
 
 
 
 
 
 
 
ITEM 1A. RISK FACTORS.

This  Annual  Report  on  Form  10-K  contains  forward-looking  statements  that  involve  risks  and  uncertainties,  such  as  statements  of  our
objectives, expectations and intentions. The cautionary statements made in this Annual Report on Form 10-K should be read as applicable to all
forward-looking statements wherever they appear in this report. Our actual results could differ materially from those discussed herein. Factors
that could cause or contribute to such differences include those discussed below, as well as those discussed elsewhere in this Annual Report on
Form 10-K.

RISKS RELATED TO OUR COMPANY

We have received an opinion from our independent registered public accounting firm expressing substantial doubt regarding our ability

to continue as a going concern.

The audit report issued by our independent registered public accounting firm on our audited consolidated financial statements for the fiscal
year ended December 31, 2017 contains an explanatory paragraph regarding our ability to continue as a going concern. The audit report states that
our auditing firm has substantial doubt in our ability to continue as a going concern due to the risk that we may not have sufficient cash and liquid
assets at December 31, 2017 to cover our operating and capital requirements for the next twelve-month period; and if sufficient cash cannot be
obtained, we would have to substantially alter, or possibly even discontinue, operations. The accompanying consolidated financial statements do
not include any adjustments that might result from the outcome of this uncertainty.

Management  has  developed  a  plan  to  continue  operations.  This  plan  includes  continued  control  of  expenses  and  obtaining  equity  or  debt
financing. Although we have successfully completed equity financings and reduced expenses in the past, we cannot assure you that our plans to
address these matters in the future will be successful.

The  factors  described  above  could  adversely  affect  our  ability  to  obtain  additional  financing  on  favorable  terms,  if  at  all,  and  may  cause
investors  to  have  reservations  about  our  long-term  prospects,  and  may  adversely  affect  our  relationships  with  customers.  There  can  be  no
assurance  that  our  auditing  firm  will  not  issue  the  same  opinion  in  the  future.  If  we  cannot  successfully  continue  as  a  going  concern,  our
stockholders may lose their entire investment.

Our revenue is dependent upon acceptance of our products by the market. The failure of such acceptance will cause us to curtail or cease

operations.

Our  revenue  comes  from  the  sale  of  our  products. As  a  result,  we  will  continue  to  incur  operating  losses  until  such  time  as  sales  of  our
products reach a mature level and we are able to generate sufficient revenue from the sale of our products to meet our operating expenses. There
can be no assurance that customers will adopt our technology and products, or that businesses and prospective customers will agree to pay for our
products. In the event that we are not able to significantly increase the number of customers that purchase our products, or if we are unable to
charge the necessary prices, our financial condition and results of operations will be materially and adversely affected.

Our business could be adversely affected if we fail to implement and maintain effective disclosure controls and procedures and internal

control over financial reporting.

We concluded that as of December 31, 2017, our disclosure controls and procedures and our internal control over financial reporting were not
effective. We have determined that we have limited resources for adequate personnel to prepare and file reports under the Securities Exchange
Act of 1934 within the required time periods and  that  material  weaknesses  in  our  internal  control  over  financial  reporting  exist  relating  to  our
accounting  for  complex  equity  transactions.  If  we  are  unable  to  implement  and  maintain  effective  disclosure  controls  and  procedures  and
remediate the material weaknesses in a timely manner, or if we identify other material weaknesses in the future, our ability to produce accurate
and  timely  financial  statements  and  public  reports  could  be  impaired,  which  could  adversely  affect  our  business  and  financial  condition.  We
identified a lack of sufficient segregation of duties. Specifically, this material weakness is such that the design over these areas relies primarily on
detective  controls  and  could  be  strengthened  by  adding  preventive  controls  to  properly  safeguard  assets.  In  addition,  investors  may  lose
confidence in our reported information and the market price of our common stock may decline.

- 20 -

 
 
 
 
 
 
 
 
 
 
 
 
 
We have a history of operating losses, anticipate future losses and may never be profitable.

We  have  experienced  significant  operating  losses  in  each  period  since  we  began  investing  resources  in  PCT  and  CP.  These  losses  have
resulted  principally  from  research  and  development,  sales  and  marketing,  and  general  and  administrative  expenses  associated  with  the
development of our PCT business. During the year ended December 31, 2017, we recorded a net loss of $10,715,561 of which approximately
$6,000,000 was interest expense, or ($9.62) per share, as compared with $2,706,984, or ($2.97) per share, of the corresponding period in 2016.
We expect to continue to incur operating losses until sales of PCT and CP products increase substantially. We cannot be certain when, if ever, we
will become profitable. Even if we were to become profitable, we might not be able to sustain such profitability on a quarterly or annual basis.

If we are unable to obtain additional financing, business operations will be harmed and if we do obtain additional financing then existing

shareholders may suffer substantial dilution.

We  need  substantial  capital  to  implement  our  sales  distribution  strategy  for  our  current  products  and  to  develop  and  commercialize  future
products using our pressure cycling technology products and services in the sample preparation area, as well as for applications in other areas of
life sciences. Our capital requirements will depend on many factors, including but not limited to:

● the problems, delays, expenses, and complications frequently encountered by early-stage companies;

● market acceptance of our pressure cycling technology products and services for sample preparation;

● the success of our sales and marketing programs; and

● changes in economic, regulatory or competitive conditions in the markets we intend to serve.

We  expect  the  net  proceeds  from  an  expected  equity  offering,  along  with  our  current  cash  position,  will  enable  us  to  fund  our  operating
expenses and capital expenditure requirements for at least the next 36 months. Thereafter, unless we achieve profitability, we anticipate that we
will need to raise additional capital to fund our operations and to otherwise implement our overall business strategy. We currently do not have any
contracts or commitments for additional financing. There can be no assurance that financing will be available in amounts or on terms acceptable
to us, if at all. Any additional equity financing may involve substantial dilution to then existing shareholders.

If adequate funds are not available or if we fail to obtain acceptable additional financing, we may be required to:

● severely limit or cease our operations or otherwise reduce planned expenditures and forego other business opportunities, which could harm

our business;

● obtain financing with terms that may have the effect of substantially diluting or adversely affecting the holdings or the rights of the holders

of our capital stock; or

● obtain funds through arrangements with future collaboration partners or others that may require us to relinquish rights to some or all of our

technologies or products.

Our financial results depend on revenues from our pressure cycling technology products and services, and from government grants.

We currently rely on revenues from PCT, CP, and CS technology products and services in the sample preparation area and from revenues
derived from grants awarded to us by governmental agencies, such as the National Institutes of Health. We have been unable to achieve market
acceptance of our product offerings to the extent necessary to achieve significant revenue. Competition for government grants is very intense, and
we can provide no assurance that we will continue to be awarded grants in the future. If we are unable to increase revenues from sales of our
pressure cycling technology products and services and government grants, our business will fail.

- 21 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
We may be unable to obtain market acceptance of our pressure cycling technology products and services.

Many of the initial sales of our pressure cycling technology products and services have been to our collaborators, following their use of our
products in studies undertaken in sample preparation for genomics, proteomics, lipidomics, and small molecules studies. Later sales have been to
key  opinion  leaders.  Our  technology  requires  scientists  and  researchers  to  adopt  a  method  of  sample  extraction  that  is  different  from  existing
techniques. Our PCT sample preparation system is also more costly than most existing techniques. Our ability to obtain market acceptance will
depend, in part, on our ability to demonstrate to our potential customers that the benefits and advantages of our technology outweigh the increased
cost of our technology compared with existing methods of sample extraction. If we are unable to demonstrate the benefits and advantages of our
products and technology as compared with existing technologies, we will not gain market acceptance and our business will fail.

Our  business  may  be  harmed  if  we  encounter  problems,  delays,  expenses,  and  complications  that  often  affect  companies  that  have  not

achieved significant market acceptance.

Our  pressure  cycling  technology  business  continues  to  face  challenges  in  achieving  market  acceptance.  If  we  encounter  problems,  delays,

expenses and complications, many of which may be beyond our control or may harm our business or prospects. These include:

● availability of adequate financing;

● unanticipated problems and costs relating to the development, testing, production, marketing, and sale of our products;

● delays and costs associated with our ability to attract and retain key personnel; and

● competition.

The sales cycle of our pressure cycling technology products is lengthy. We have incurred and may continue to incur significant expenses

and we may not generate any significant revenue related to those products.

Many of our current and potential customers have required between three and six months or more to test and evaluate our pressure cycling
technology  products.  This  increases  the  possibility  that  a  customer  may  decide  to  cancel  its  order  or  otherwise  change  its  plans,  which  could
reduce  or  eliminate  our  sales  to  that  potential  customer. As  a  result  of  this  lengthy  sales  cycle,  we  have  incurred  and  may  continue  to  incur
significant research and development, selling and marketing, and general and administrative expense related to customers from whom we have
not yet generated any revenue from our products, and from whom we may never generate the anticipated revenue if a customer is not satisfied
with the results of the evaluation of our products or if a customer cancels or changes its plans.

Our business could be harmed if our products contain undetected errors or defects.

We are continuously developing new and improving our existing, pressure cycling technology products in sample preparation and we expect
to do so in other areas of life sciences depending upon the availability of our resources. Newly introduced products can contain undetected errors
or  defects.  In  addition,  these  products  may  not  meet  their  performance  specifications  under  all  conditions  or  for  all  applications.  If,  despite
internal testing and testing by our collaborators, any of our products contain errors or defects or fail to meet customer specifications, then we may
be required to enhance or improve those products or technologies. We may not be able to do so on a timely basis, if at all, and may only be able to
do  so  at  considerable  expense.  In  addition,  any  significant  reliability  problems  could  result  in  adverse  customer  reaction,  negative  publicity  or
legal claims and could harm our business and prospects.

Our success may depend on our ability to manage growth effectively.

Our failure to manage growth effectively could harm our business and prospects. Given our limited resources and personnel, growth of our
business  could  place  significant  strain  on  our  management,  information  technology  systems,  sources  of  manufacturing  capacity  and  other
resources. To properly manage our growth, we may need to hire additional employees and identify new sources of manufacturing capabilities.
Failure to effectively manage our growth could make it difficult to manufacture our products and fill orders, as well as lead to declines in product
quality or increased costs, any of which would adversely impact our business and results of operations.

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Our success is substantially dependent on the continued service of our senior management.

Our success is substantially dependent on the continued service of our senior management, specifically our Chief Executive Officer, Richard
T. Schumacher. The loss of the services of any of our senior management could make it more difficult to successfully operate our business and
achieve  our  business  goals.  In  addition,  our  failure  to  retain  existing  engineering,  research  and  development,  operations,  and  marketing/sales
personnel could harm our product development capabilities and customer and employee relationships, delay the growth of sales of our products,
and result in the loss of key information, expertise, or know-how.

We may not be able to hire or retain the number of qualified personnel, particularly engineering and sales personnel, required for our

business, which would harm the development and sales of our products and limit our ability to grow.

Competition in our industry for senior management, technical, sales, marketing, finance and other key personnel is intense. If we are unable to
retain our existing personnel, or attract and train additional qualified personnel, either because of competition in our industry for such personnel or
because of insufficient financial resources, our growth may be limited. Our success also depends in particular on our ability to identify, hire, train
and retain qualified engineering and sales personnel with experience in design, development and sales of laboratory equipment.

Our reliance on a single third party for all of our manufacturing, and certain of our engineering, and other related services could harm

our business.

We currently solely rely on CBM Industries, a third party contract manufacturer, to manufacture our Barocycler 2320EXT instrumentation,
provide  manufacturing  expertise,  and  manage  the  majority  of  our  sub-contractor  supplier  relationships  for  this  instrument.  Because  of  our
dependence  on  one  manufacturer,  our  success  will  depend,  in  part,  on  the  ability  of  CBM  to  manufacture  our  products  cost  effectively,  in
sufficient quantities to meet our customer demand, if and when such demand occurs, and meeting our quality requirements. If CBM experiences
manufacturing problems or delays, or if CBM decides not to continue to provide us with these services, our business may be harmed. While we
believe other contract manufacturers are available to address our manufacturing and engineering needs, if we find it necessary to replace CBM,
there will be a disruption in our business and we would incur additional costs and delays that would harm our business.

Our failure to manage current or future alliances or joint ventures effectively may harm our business.

We have entered into business relationships with four distribution partners and one co-marketing partner, and we may enter into additional
alliances, joint ventures or other business relationships to further develop, market and sell our pressure cycling technology product line. We may
not be able to:

● identify appropriate candidates for alliances, joint ventures or other business relationships;

● assure that any candidate for an alliance, joint venture or business relationship will provide us with the support anticipated;

● successfully negotiate an alliance, joint venture or business relationship on terms that are advantageous to us; or

● successfully manage any alliance or joint venture.

Furthermore,  any  alliance,  joint  venture  or  other  business  relationship  may  divert  management  time  and  resources.  Entering  into  a
disadvantageous alliance, joint venture or business relationship, failing to manage an alliance, joint venture or business relationship effectively, or
failing to comply with any obligations in connection therewith, could harm our business and prospects.

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We may not be successful in growing our international sales.

We  cannot  guarantee  that  we  will  successfully  develop  our  international  sales  channels  to  enable  us  to  generate  significant  revenue  from
international sales. We currently have four international distribution agreements that cover 24 countries in Europe, Asia and Australia. We have
generated  limited  sales  to  date  from  international  sales  and  cannot  guarantee  that  we  will  be  able  to  increase  our  sales. As  we  expand,  our
international operations may be subject to numerous risks and challenges, including:

● multiple, conflicting and changing governmental laws and regulations, including those that regulate high pressure equipment;

● reduced protection for intellectual property rights in some countries;

● protectionist laws and business practices that favor local companies;

● political and economic changes and disruptions;

● export and import controls;

● tariff regulations; and

● currency fluctuations.

Our operating results are subject to quarterly variation. Our operating results may fluctuate significantly from period to period depending

on a variety of factors, including but not limited to the following:

● our ability  to  increase  our  sales  of  our  pressure  cycling  technology  products  for  sample  preparation  on  a  consistent  quarterly or  annual

basis;

● the lengthy sales cycle for our products;

● the product mix of the Barocycler® instruments we install in a given period, and whether the installations are completed pursuant to sales,

rental or lease arrangements, and the average selling prices that we are able to command for our products;

● our ability to manage our costs and expenses;

● our ability to continue our research and development activities without incurring unexpected costs and expenses; and

● our ability to comply with state and federal regulations without incurring unexpected costs and expenses.

Our instrumentation operates at high pressures and may therefore become subject to certain  regulations  in  the  European  Community.

Regulation of high pressure equipment may limit or hinder our development and sale of future instrumentation.

Our  Barocycler®  instruments  operate  at  high  pressures.  If  our  Barocycler®  instruments  exceed  certain  pressure  levels,  our  products  may
become  subject  to  the  European  Pressure  Equipment  Directive,  which  requires  certain  pressure  equipment  meet  certain  quality  and  safety
standards.  We  do  not  believe  that  we  are  subject  to  this  directive  because  our  Barocycler®  instruments  are  currently  below  the  threshold
documented in the text of the directive. If our interpretation were to be challenged, we could incur significant costs defending the challenge, and
we could face production and selling delays, all of which could harm our business.

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We expect that we will be subject to regulation in the United States, such as by the Food and Drug Administration, and overseas, if and
when we begin to invest more resources in the development and commercialization of PCT in applications outside of sample preparation for
the research field.

Our  current  pressure  cycling  technology  products  in  the  area  of  sample  preparation  for  the  research  field  are  not  regulated  by  the  FDA.
Certain  applications  in  which  we  intend  to  develop  and  commercialize  pressure  cycling  technology,  such  as  protein  purification,  pathogen
inactivation and immunodiagnostics, are expected to require regulatory approvals or clearances from regulatory agencies, such as the FDA, prior
to commercialization, when we expand our commercialization activities outside of the research field. We expect that obtaining these approvals or
clearances  will  require  a  significant  investment  of  time  and  capital  resources  and  there  can  be  no  assurance  that  such  investments  will  receive
approvals or clearances that would allow us to commercialize the technology for these applications.

If we are unable to protect our patents and other proprietary technology relating to our pressure cycling technology products, our business

will be harmed.

Our  ability  to  further  develop  and  successfully  commercialize  our  products  will  depend,  in  part,  on  our  ability  to  enforce  our  patents,
preserve our trade secrets, and operate without infringing the proprietary rights of third parties. To date, we have been granted 15 United States
and foreign patents related to our PCT technology platform, and two additional patents in China related to our Ultra Shear Technology. We also
received eight patents with our purchase of the assets of BaroFold in December 2017.

There can be no assurance that (a) any patent applications filed by us will result in issued patents; (b) patent protection will be secured for any
particular technology; (c) any patents that have been or may be issued to us will be valid or enforceable; (d) any patents will provide meaningful
protection  to  us;  (e)  others  will  not  be  able  to  design  around  our  patents;  and  (f)  our  patents  will  provide  a  competitive  advantage  or  have
commercial  value.  The  failure  to  obtain  adequate  patent  protection  would  have  a  material  adverse  effect  on  us  and  may  adversely  affect  our
ability to enter into, or affect the terms of, any arrangement for the marketing or sale of any product.

Our patents may be challenged by others.

We  could  incur  substantial  costs  in  patent  proceedings,  including  interference  proceedings  before  the  United  States  Patent  and  Trademark
Office, and comparable proceedings before similar agencies in other countries, in connection with any claims that may arise in the future. These
proceedings could result in adverse decisions about the patentability of our inventions and products, as well as about the enforceability, validity,
or scope of protection afforded by the patents.

If  we  are  unable  to  maintain  the  confidentiality  of  our  trade  secrets  and  proprietary  knowledge,  others  may  develop  technology  and

products that could prevent the successful commercialization of our products.

We rely on trade secrets and other unpatented proprietary information in our product development activities. To the extent we rely on trade
secrets  and  unpatented  know-how  to  maintain  our  competitive  technological  position,  there  can  be  no  assurance  that  others  may  not
independently  develop  the  same  or  similar  technologies.  We  seek  to  protect  our  trade  secrets  and  proprietary  knowledge,  in  part,  through
confidentiality  agreements  with  our  employees,  consultants,  advisors  and  contractors.  These  agreements  may  not  be  sufficient  to  effectively
prevent disclosure of our confidential information and may not provide us with an adequate remedy in the event of unauthorized disclosure of
such information. If our employees, consultants, advisors, or contractors develop inventions or processes independently that may be applicable to
our products, disputes may arise about ownership of proprietary rights to those inventions and processes. Such inventions and processes will not
necessarily  become  our  property,  but  may  remain  the  property  of  those  persons  or  their  employers.  Protracted  and  costly  litigation  could  be
necessary to enforce and determine the scope of our proprietary rights. Failure to obtain or maintain trade secret protection, for any reason, could
harm our business.

If we infringe on the intellectual property rights of others, our business may be harmed.

It is possible that the manufacture, use or sale of our pressure cycling technology products or services may infringe patent or other intellectual
property rights of others. We may be unable to avoid infringement of the patent or other intellectual property rights of others and may be required
to seek a license, defend an infringement action, or challenge the validity of the patents or other intellectual property rights in court. We may be
unable to secure a license on terms and conditions acceptable to us, if at all. Also, we may not prevail in any patent or other intellectual property
rights litigation. Patent or other intellectual property rights litigation is costly and time-consuming, and there can be no assurance that we will
have sufficient resources to bring any possible litigation related to such infringement to a successful conclusion. If we do not obtain a license under
such  patents  or  other  intellectual  property  rights,  or  if  we  are  found  liable  for  infringement,  or  if  we  are  unsuccessful  in  having  such  patents
declared  invalid,  we  may  be  liable  for  significant  monetary  damages,  may  encounter  significant  delays  in  successfully  commercializing  and
developing  our  pressure  cycling  technology  products,  or  may  be  precluded  from  participating  in  the  manufacture,  use,  or  sale  of  our  pressure
cycling technology products or services requiring such licenses.

- 25 -

 
 
 
 
 
 
 
 
 
 
 
 
 
We may be unable to adequately respond to rapid changes in technology and the development of new industry standards.

The introduction of products and services embodying new technology and the emergence of new industry standards may render our existing
pressure cycling technology products and related services obsolete and unmarketable if we are unable to adapt to change. We may be unable to
allocate  the  funds  necessary  to  improve  our  current  products  or  introduce  new  products  to  address  our  customers’  needs  and  respond  to
technological  change.  In  the  event  that  other  companies  develop  more  technologically  advanced  products,  our  competitive  position  relative  to
such companies would be harmed.

We may not be able to compete successfully with others that are developing or have developed competitive technologies and products.

A number of companies have developed, or are expected to develop, products that compete or will compete with our products. We compete
with companies that have existing technologies for the extraction of nucleic acids, proteins and small molecules from cells and tissues, including
but  not  limited  to  methods  such  as  mortar  and  pestle,  sonication,  rotor-stator  homogenization,  French  press,  bead  beating,  freezer  milling,
enzymatic digestion, and chemical dissolution.

We are aware that there are additional companies pursuing new technologies with similar goals to the products developed or being developed
by us. Some of the companies with which we now compete, or may compete in the future, have or may have more extensive research, marketing,
and  manufacturing  capabilities,  more  experience  in  genomics  and  proteomics  sample  preparation,  protein  purification,  pathogen  inactivation,
immunodiagnostics, and DNA sequencing and significantly greater technical, personnel and financial resources than we do, and may be better
positioned to continue to improve their technology to compete in an evolving industry. To compete, we must be able to demonstrate to potential
customers  that  our  products  provide  improved  performance  and  capabilities.  Our  failure  to  compete  successfully  could  harm  our  business  and
prospects.

We will need to increase the size of our organization, and may experience difficulties in managing growth.

We  are  a  small  company  with  a  minimal  number  of  employees.  We  expect  to  experience  a  period  of  expansion  in  headcount,  facilities,
infrastructure and overhead and anticipate that further expansion will be required to address potential growth and market opportunities. Future
growth will impose significant added responsibilities on members of management, including the need to identify, recruit, maintain and integrate
new managers. Our future financial performance and its ability to compete effectively will depend, in part, on its ability to manage any future
growth effectively.

Provisions in our articles of organization and bylaws may discourage or frustrate stockholders’ attempts to remove or replace our current

management.

Our articles of organization and bylaws contain provisions that may make it more difficult or discourage changes in our management that our

stockholders may consider to be favorable. These provisions include:

● a classified board of directors;

● advance notice for stockholder nominations to the board of directors;

● limitations on the ability of stockholders to remove directors; and

● a provision that allows a majority of the directors to fill vacancies on the board of directors.

- 26 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
These provisions could prevent or frustrate attempts to make changes in our management that our stockholders consider to be beneficial and

could limit the price that our stockholders might receive in the future for shares of our common stock.

The costs of compliance with the reporting obligations of the Exchange Act, and with the requirements of the Sarbanes-Oxley Act of 2002
and the Dodd-Frank Wall Street Reform and Consumer Protection Act, may place a strain on our limited resources and our management’s
attention may be diverted from other business concerns.

As a result of the regulatory requirements applicable to public companies, we incur legal, accounting, and other expenses that are significant
in  relation  to  the  size  of  our  Company.  In  addition,  the  Sarbanes-Oxley Act  of  2002  and  the  Dodd-Frank  Wall  Street  Reform  and  Consumer
Protection Act, as well as rules subsequently implemented by the SEC and OTC Markets Group, Inc., have increased and will continue to increase
our legal and financial compliance costs and may make some activities more time-consuming. These requirements have placed and will continue
to place a strain on our systems and on our management and financial resources.

Certain  of  our  net  deferred  tax  assets  could  be  substantially  limited  if  we  experience  an  ownership  change  as  defined  in  the  Internal

Revenue Code.

Certain of our net operating losses (“NOLs”) give rise to net deferred tax assets. Our ability to utilize NOLs and to offset our future taxable
income and/or to recover previously paid taxes would be limited if we were to undergo an “ownership change” within the meaning of Section 382
of  the  Internal  Revenue  Code  (the  “Code”).  In  general,  an  “ownership  change”  occurs  whenever  the  percentage  of  the  stock  of  a  corporation
owned by “5 percent shareholders,” within the meaning of Section 382 of the Code, increases by more than 50 percentage points over the lowest
percentage of the stock of such corporation owned by such “5 percent shareholders” at any time over the preceding three years.

An ownership change under Section 382 of the Code would establish an annual limitation on the amount of NOLs we could utilize to offset
our taxable income in any single taxable year to an amount equal to (i) the product of a specified rate, which is published by the U.S. Treasury,
and  the  aggregate  value  of  our  outstanding  stock  plus;  and  (ii)  the  amount  of  unutilized  limitation  from  prior  years.  The  application  of  these
limitations might prevent full utilization of the deferred tax assets attributable to our NOLs. We may have or will have experienced an ownership
change  as  defined  by  Section  382  through  the  sale  of  equity  and,  therefore,  we  will  consider  whether  the  sale  of  equity  units  will  result  in
limitations  of  our  net  operating  losses  under  Section  382  when  we  start  to  generate  taxable  income.  However,  whether  a  change  in  ownership
occurs in the future is largely outside of our control, and there can be no assurance that such a change will not occur.

Significant policy shifts from the Trump Administration could have a material adverse effect on us.

The  Trump  Administration  has  called  for  substantial  change  to  fiscal  and  tax  policies,  regulatory  oversight  of  businesses,  and  greater
restrictions  on  free  trade  including  significant  increases  on  tariffs  on  goods  imported  into  the  United  States,  including  from  China.  Proposals
espoused by President Trump may result in changes to social, political, regulatory and economic conditions in the United States or in laws and
policies affecting the development and investment in countries where we currently conduct business. In addition, these changes could result in
negative sentiments towards the United States among non-U.S. customers and among non-U.S. employees or prospective employees. We cannot
predict the impact, if any, of these changes to our business. However, it is possible that these changes could adversely affect our business. It is
likely that some policies adopted by the new administration will benefit us and others will negatively affect us.

RISKS RELATING TO OWNERSHIP OF OUR SECURITIES

The holders of our Common Stock could suffer substantial dilution due to our corporate financing practices.

The holders of our common stock could suffer substantial dilution due to our corporate financing practices, which, in the past few years, have
included private placements and a registered direct offering. As of December 31, 2017, we have issued shares of Series A Convertible Preferred
Stock, Series B Convertible Preferred Stock, Series C Convertible Preferred Stock, Series D Convertible Preferred Stock, Series E Convertible
Preferred  Stock,  Series  G  Convertible  Preferred  Stock,  Series  H  Convertible  Preferred  Stock,  Series  H2  Convertible  Preferred  Stock,  Series  J
Convertible Preferred Stock and Series K Convertible Preferred Stock.

- 27 -

 
 
 
 
 
 
 
 
 
 
 
 
As of December 31, 2017, all of the issued shares of Series A Convertible Preferred Stock, Series B Convertible Preferred Stock, Series C
Convertible  Preferred  Stock,  and  Series  E  Convertible  Preferred  Stock  had  been  converted  into  shares  of  common  stock. As  of  December  31,
2017 only shares of Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H Convertible Preferred Stock, Series H2
Convertible  Preferred  Stock,  Series  J  Convertible  Preferred  Stock  and  Series  K  Convertible  Preferred  Stock  were  outstanding.  Further,  in
connection with those private placements and the Series D registered direct offering, we issued warrants to purchase common stock. In addition,
as of December 31, 2017, we had issued notes and debentures convertible into common stock at prices ranging from $7.50 to $8.40 per common
share.  If  all  of  the  outstanding  shares  of  Series  D  Convertible  Preferred  Stock,  Series  G  Convertible  Preferred  Stock,  Series  H  Convertible
Preferred  Stock,  Series  H2  Convertible  Preferred  Stock,  Series  J  Convertible  Preferred  Stock  and  Series  K  Convertible  Preferred  Stock  were
converted into shares of common stock and all outstanding options and warrants to purchase shares of common stock were exercised and all fixed
rate convertible notes and debentures were converted, each as of December 31, 2017, an additional 2,594,229 shares of common stock would be
issued  and  outstanding.  This  additional  issuance  of  shares  of  common  stock  would  cause  immediate  and  substantial  dilution  to  our  existing
stockholders and could cause a significant reduction in the market price of our common stock.

Sales of a significant number of shares of our common stock in the public market or the perception of such possible sales, could depress

the market price of our common stock.

Sales  of  a  substantial  number  of  shares  of  our  common  stock  in  the  public  markets,  which  include  an  offering  of  our  preferred  stock  or
common stock could depress the market price of our common stock and impair our ability to raise capital through the sale of additional equity or
equity-related securities. We cannot predict the effect that future sales of our common stock or other equity-related securities would have on the
market price of our common stock.

Our share price could be volatile and our trading volume may fluctuate substantially.

The price of common stock has been and may in the future continue to be extremely volatile. Many factors could have a significant impact on

the future price of our shares of common stock, including:

● our inability to raise additional capital to fund our operations, whether through the issuance of equity securities or debt;

● our failure to successfully implement our business objectives;

● compliance with ongoing regulatory requirements;

● market acceptance of our products;

● technological innovations and new commercial products by our competitors;

● changes in government regulations;

● general economic conditions and other external factors;

● actual or anticipated fluctuations in our quarterly financial and operating results; and

● the degree of trading liquidity in our shares of common stock.

A decline in the price of our shares of common stock could affect our ability to raise further working capital and adversely impact our

ability to continue operations.

The relatively low price of our shares of common stock, and a decline in the price of our shares of common stock, could result in a reduction
in the liquidity of our common stock and a reduction in our ability to raise capital. Because a significant portion of our operations has been and
will  continue  to  be  financed  through  the  sale  of  equity  securities,  a  decline  in  the  price  of  our  shares  of  common  stock  could  be  especially
detrimental to our liquidity and our operations. Such reductions and declines may force us to reallocate funds from other planned uses and may
have a significant negative effect on our business plans and operations, including our ability to continue our current operations. If the price for our
shares of common stock declines, it may be more difficult to raise additional capital. If we are unable to raise sufficient capital, and we are unable
to generate funds from operations sufficient to meet our obligations, we will not have the resources to continue our operations.

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The market price for our shares of common stock may also be affected by our ability to meet or exceed expectations of analysts or investors.

Any failure to meet these expectations, even if minor, may have a material adverse effect on the market price of our shares of common stock.

If we issue additional securities in the future, it will likely result in the dilution of our shares of existing stockholders.

As of December 31, 2017, there were 1,342,858 shares of common stock issued and outstanding. Similarly, at such time, there were no shares
of  Series  A  Junior  Participating  Preferred  Stock;  Series  A  Convertible  Preferred  Stock;  Series  B  Convertible  Preferred  Stock;  Series  C
Convertible Preferred Stock; and Series E Convertible Preferred Stock. As of December 31, 2017 there were 300 shares of Series D Convertible
Preferred  Stock  issued  and  outstanding  and  convertible  into  25,000  shares  of  common  stock,  80,570  shares  of  Series  G  Convertible  Preferred
Stock issued and outstanding convertible into 26,857 shares of common stock, 10,000 shares of Series H Convertible Preferred Stock issued and
outstanding  convertible  into  33,334  shares  of  common  stock,  21  shares  of  Series  H2  Convertible  Preferred  Stock  issued  and  outstanding
convertible  into  70,000  shares  of  common  stock,  3,458  shares  of  Series  J  Convertible  Preferred  Stock  issued  and  outstanding  convertible  into
115,267  shares  of  common  stock,  and  6,880  shares  of  Series  K  Convertible  Preferred  Stock  issued  and  outstanding  convertible  into  229,334
shares of common stock.

As of December 31, 2017, there were outstanding options and warrants to purchase an aggregate of 1,147,234 shares of common stock; and
fixed  rate  convertible  debt  convertible  into  947,203  shares  of  common  stock.  From  time  to  time,  we  also  may  increase  the  number  of  shares
available for issuance in connection with our equity compensation plan, we may adopt new equity compensation plans, and we may issue awards
to our employees and others who provide services to us outside the terms of our equity compensation plans. Our board of directors may fix and
determine the designations, rights, preferences or other variations of each class or series of preferred stock and may choose to issue some or all of
such shares to provide additional financing in the future.

The issuance of any securities for acquisition, licensing or financing efforts, upon conversion of any preferred stock or exercise of warrants,
pursuant to our equity compensation plans, or otherwise may result in a reduction of the book value and market price of the outstanding shares of
our  common  stock.  If  we  issue  any  such  additional  securities,  such  issuance  will  cause  a  reduction  in  the  proportionate  ownership  and  voting
power of all current stockholders. Further, such issuance may result in a change in control of our Company.

Financial Industry Regulatory Authority (“FINRA”) sales practice requirements may also limit a stockholder’s ability to buy and sell our

common stock.

FINRA has adopted rules that require that in recommending an investment to a customer, a broker-dealer must have reasonable grounds for
believing  that  the  investment  is  suitable  for  that  customer.  Prior  to  recommending  speculative  low-priced  securities  to  their  non-institutional
customers,  broker-dealers  must  make  reasonable  efforts  to  obtain  information  about  the  customer’s  financial  status,  tax  status,  investment
objectives and other information. Under interpretations of these rules, FINRA believes that there is a high probability that speculative low-priced
securities will not be suitable for at least some customers. FINRA requirements make it more difficult for broker-dealers to recommend that their
customers buy our common stock, which may limit your ability to buy and sell our common stock and have an adverse effect on the market for
our shares.

- 29 -

 
 
 
 
 
 
 
 
 
Our Common Stock is subject to the “Penny Stock” rules of the SEC and the trading market in our securities is limited, which makes

transactions in our stock cumbersome and may reduce the value of an investment in our stock.

The  Securities  and  Exchange  Commission  has  adopted  Rule  15g-9  which  establishes  the  definition  of  a  “penny  stock,”  for  the  purposes
relevant to us, as any equity security that has a market price of less than $5.00 per share or with an exercise price of less than $5.00 per share,
subject to certain exceptions. For any transaction involving a penny stock, unless exempt, the rules require:

● That a broker or dealer approve a person’s account for transactions in penny stocks; and

● The broker or dealer receives from the investor a written agreement to the transaction, setting forth the identity and quantity of the penny

stock to be purchased.

In order to approve a person’s account for transactions in penny stocks, the broker or dealer must:

● Obtain financial information and investment experience objectives of the person; and

● Make a reasonable determination that the transactions in penny stocks are suitable for that person and the person has sufficient knowledge

and experience in financial matters to be capable of evaluating the risks of transactions in penny stocks.

The broker or dealer must also deliver, prior to any transaction in a penny stock, a disclosure schedule prescribed by the Commission relating

to the penny stock market, which, in highlight form:

● Sets forth the basis on which the broker or dealer made the suitability determination; and

● That the broker or dealer received a signed, written agreement from the investor prior to the transaction.

Generally, brokers may be less willing to execute transactions in securities subject to the “penny stock” rules. This may make it more difficult

for investors to dispose of our common stock and cause a decline in the market value of our stock.

Disclosure also has to be made about the risks of investing in penny stocks in both public offerings and in secondary trading and about the
commissions payable to both the broker-dealer and the registered representative, current quotations for the securities and the rights and remedies
available  to  an  investor  in  cases  of  fraud  in  penny  stock  transactions.  Finally,  monthly  statements  have  to  be  sent  disclosing  recent  price
information for the penny stock held in the account and information on the limited market in penny stocks.

We have never declared or paid a cash dividend on our common stock and we do not expect to pay cash dividends on our common stock in

the foreseeable future.

Our  shares  of  Series  D  Convertible  Preferred  Stock  are  entitled  to  certain  rights,  privileges  and  preferences  over  our  common  stock,
including a preference upon a liquidation of our Company, which will reduce amounts available for distribution to the holders of our common
stock.

The holders of our shares of Series D are entitled to payment, prior to payment to the holders of common stock in the event of liquidation of
the  Company. If we are dissolved, liquidated or wound up at a time when the Series D Preferred Stock remain outstanding, the holders of the
Series D Preferred Stock will be entitled to receive only an amount equal to the liquidation preference (as it may be adjusted from time to time),
plus  any  accumulated  and  unpaid  dividends,  to  the  extent  that  we  have  funds  legally  available. Any  remaining  assets  will  be  distributable  to
holders of our other equity securities.

- 30 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Shares eligible for future sale may adversely affect the market.

From  time  to  time,  certain  of  our  stockholders  may  be  eligible  to  sell  all  or  some  of  their  shares  of  common  stock  by  means  of  ordinary
brokerage transactions in the open market pursuant to Rule 144 promulgated under the Securities Act, subject to certain limitations. In general,
pursuant  to  amended  Rule  144,  non-affiliate  stockholders  may  sell  freely  after  six  months  subject  only  to  the  current  public  information
requirement. Affiliates may sell after six months subject to the Rule 144 volume, manner of sale (for equity securities), current public information
and notice requirements. Any substantial sales of our common stock pursuant to Rule 144 may have a material adverse effect on the market price
of our common stock.

We currently do not intend to pay dividends on our common stock. As result, your only opportunity to achieve a return on your investment

is if the price of our common stock appreciates.

We currently do not expect to declare or pay dividends on our common stock. In addition, in the future we may enter into agreements that
prohibit or restrict our ability to declare or pay dividends on our common stock. As a result, your only opportunity to achieve a return on your
investment will be if the market price of our common stock appreciates and you sell your shares at a profit.

We could issue additional common stock, which might dilute the book value of our Common Stock.

Our Board of Directors has authority, without action or vote of our shareholders, to issue all or a part of our authorized but unissued shares.
Such stock issuances could be made at a price that reflects a discount or a premium from the then-current trading price of our common stock. In
addition, in order to raise capital, we may need to issue securities that are convertible into or exchangeable for our common stock. These issuances
would  dilute  the  percentage  ownership  interest,  which  would  have  the  effect  of  reducing  your  influence  on  matters  on  which  our  shareholders
vote, and might dilute the book value of our common stock. You may incur additional dilution if holders of stock warrants or options, whether
currently  outstanding  or  subsequently  granted,  exercise  their  options,  or  if  warrant  holders  exercise  their  warrants  to  purchase  shares  of  our
common stock.

ITEM 1B. UNRESOLVED STAFF COMMENTS.

Not Applicable.

ITEM 2. PROPERTIES.

Our  corporate  office  is  currently  located  at  14  Norfolk Avenue,  South  Easton,  Massachusetts  02375.  We  are  currently  paying  $6,950  per
month, on a lease extension, signed on December 29, 2017, that expires December 31, 2018, for our corporate office. We expanded our space to
include offices, warehouse and a loading dock on the first floor starting May 1, 2017 with a monthly rent increase already reflected in the current
payments.

On  October  18,  2017  we  signed  a  lease  extension  for  our  lab  space  in  Medford,  MA.  The  lease  will  now  expire  December  30,  2020  and
requires  monthly  payments  of  $6,912.75  starting  January  1,  2018  subject  to  annual  cost  of  living  increases.  The  lease  shall  be  automatically
extended for additional three years unless either party terminates at least six months prior to the expiration of the current lease term.

ITEM 3. LEGAL PROCEEDINGS.

We are not currently involved in any litigation that we believe could have a material adverse effect on our financial condition or results of
operations. There is no action, suit, or proceeding by any court, public board, government agency, self-regulatory organization or body pending or,
to the knowledge of the executive officers of our Company or our subsidiary, threatened against or affecting our Company, our common stock,
our subsidiary or of our companies or our subsidiary’s officers or directors in their capacities as such, in which an adverse decision could have a
material adverse effect.

ITEM 4. MINE SAFETY DISCLOSURES.

Not applicable.

- 31 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
PART II

ITEM  5.  MARKET  FOR  REGISTRANT’S  COMMON  EQUITY,  RELATED  STOCKHOLDER  MATTERS,  AND  ISSUER
PURCHASES OF EQUITY SECURITIES.

Our common stock is currently traded on the OTCQB tier of the OTC Markets under the trading symbol “PBIO.”

The following table sets forth, for the periods indicated, the high and low sales price and the high and low bids, as applicable, per share of

common stock, as reported by the OTC Markets from January 1, 2016 through December 31, 2017.

First Quarter
Second Quarter
Third Quarter
Fourth Quarter

First Quarter
Second Quarter
Third Quarter
Fourth Quarter

Authorized Capital

Year Ended December 31, 2017

High

Low

11.40    $
10.50    $
7.90    $
4.85    $

Year Ended December 31, 2016

High

Low

15.30    $
17.40    $
13.80    $
12.00    $

4.50 
4.60 
0.70 
2.95 

8.40 
7.80 
8.40 
5.40 

$
$
$
$

$
$
$
$

As  of  December  31,  2017,  we  were  authorized  to  issue  100,000,000  shares  of  common  stock,  $.01  par  value,  and  1,000,000  shares  of
preferred  stock,  $.01  par  value.  Of  the  1,000,000  shares  of  preferred  stock,  20,000  shares  were  designated  as  Series A  Junior  Participating
Preferred Stock, 313,960 shares as Series A Convertible Preferred Stock, 279,256 shares as Series B Convertible Preferred Stock, 88,098 shares
as Series C Convertible Preferred Stock, 850 shares as Series D Convertible Preferred Stock, 500 shares as Series E Convertible Preferred Stock,
240,000  shares  as  Series  G  Convertible  Preferred  Stock,  10,000  shares  as  Series  H  Convertible  Preferred  Stock,  21  shares  as  Series  H2
Convertible Preferred Stock, 6,250 shares as Series J Convertible Preferred Stock and 15,000 shares as Series K Convertible Preferred Stock.

As of December 31, 2017, there were 1,342,858 shares of common stock issued and outstanding. Similarly, at such time, there were no shares
of  Series  A  Junior  Participating  Preferred  Stock;  Series  A  Convertible  Preferred  Stock;  Series  B  Convertible  Preferred  Stock;  Series  C
Convertible Preferred Stock; and Series E Convertible Preferred Stock. As of December 31, 2017 there were 300 shares of Series D Convertible
Preferred  Stock  issued  and  outstanding  and  convertible  into  25,000  shares  of  common  stock,  80,570  shares  of  Series  G  Convertible  Preferred
Stock issued and outstanding convertible into 26,857 shares of common stock, 10,000 shares of Series H Convertible Preferred Stock issued and
outstanding  convertible  into  33,334  shares  of  common  stock,  21  shares  of  Series  H2  Convertible  Preferred  Stock  issued  and  outstanding
convertible  into  70,000  shares  of  common  stock,  3,458  shares  of  Series  J  Convertible  Preferred  Stock  issued  and  outstanding  convertible  into
115,267  shares  of  common  stock,  and  6,880  shares  of  Series  K  Convertible  Preferred  Stock  issued  and  outstanding  convertible  into  229,334
shares of common stock.

Approximate Number of Equity Security Holders

As  of  December  31,  2017,  there  were  approximately  205  stockholders  of  record.  Because  shares  of  our  common  stock  are  held  by
depositaries, brokers and other nominees, the number of beneficial holders of our shares is substantially larger than the number of stockholders of
record.

Dividends

We have never declared or paid any cash dividends on common stock and do not plan to pay any cash dividends on common stock in the

foreseeable future.

- 32 -

 
 
 
 
 
 
 
 
 
 
 
   
 
 
 
 
 
 
 
 
 
 
 
   
 
 
 
 
 
 
 
 
 
 
 
 
 
As of December 31, 2017, dividends issued or to be issued on convertible preferred stock for the years ended December 31, 2017 and 2016

are outlined in the table below.

Series D
Series E
Series G
Series H
Series H2
Series J
Series K

Dividends paid in common stock or cash
For The Year Ended December 31,

2017

2016

Dividends payable
For The Year Ended December 31,

2017

2016

  $

  $

-    $
-     
-     
-     
-     
-     
-     
-    $

-    Series D
-    Series E
-    Series G
-    Series H
-    Series H2

442    Series J
63,413    Series K
63,855     

  $

  $

-    $
-     
-     
-     
-     
83,004     
107,478     
190,482    $

- 
- 
1,200 
- 
- 
83,484 
108,620 
193,304 

Unregistered Sales of Equity Securities and Use of Proceeds

  During  the  year  ended  December  31,  2017,  we  issued  securities  that  were  not  registered  under  the  Securities  Act,  and  were  not
previously  disclosed  in  a  Quarterly  Report  on  Form  10-Q  or  a  Current  Report  on  Form  8-K  as  listed  below.  Except  where  noted,  all  of  the
securities discussed in this Item 5 were issued in reliance on the exemption under Section 4(a)(2) of the Securities Act.

On September 20, 2017, we issued 4,000 shares of restricted common stock to an investor relations firm and recorded the common stock’s fair
value of $16,000 as administrative expense in the year ended December 31, 2017.

October 19, 2017 the Company issued 2,500 shares of its Common Stock at $4.00 per share to a lender for a loan in the amount of $250,000.

On October 27, 2017 the Company issued 2,500 shares of its Common Stock at $4.00 per share to a lender. In consideration for a loan in the
amount of $170,000.

On November 15, 2017 the Company issued to Debenture holders 22,701 shares of its Common Stock for quarterly interest of $173,589 issued in
stock in lieu of cash.

On December 11, 2017 the Company issued 1,700 shares of its Common Stock to a lender for a loan in the amount of $130,000.

On December 11, 2017 the Company issued 2,500 shares of its Common Stock to a lender for an extension on a loan of $170,000 until February
15, 2018.

On  November  29,  2017  the  Company  issued  4,000  shares  of  its  Common  Stock  at  $3.80  per  share  to  a  lender  for  a  loan  in  the  amount  of
$150,000.

On  December  19,  2017  the  Company  issued  1,500  shares  of  its  Common  Stock  at  $3.85  per  share  to  a  lender  for  a  loan  in  the  amount  of
$110,000.

On December 31, 2017, the Company awarded 2,134 shares of common stock at $7.50 per share to an investor relations firm for services to be
rendered in 2018 for the Company.

ITEM 6. SELECTED FINANCIAL DATA.

Not Applicable.

- 33 -

 
 
 
 
 
 
 
   
   
 
 
   
 
   
   
   
   
   
   
   
   
   
   
   
   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
ITEM 7. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATION.

OVERVIEW

We  are  focused  on  solving  the  challenging  problems  inherent  in  biological  sample  preparation,  a  crucial  laboratory  step  performed  by
scientists worldwide working in biological life sciences research. Sample preparation is a term that refers to a wide range of activities that precede
most forms of scientific analysis. Sample preparation is often complex, time-consuming and, in our belief, one of the most error-prone steps of
scientific research. It is a widely-used laboratory undertaking – the requirements of which drive what we believe is a large and growing worldwide
market. We have developed and patented a novel, enabling technology platform that can control the sample preparation process. It is based on
harnessing  the  unique  properties  of  high  hydrostatic  pressure.  This  process,  which  we  refer  to  as  PCT,  uses  alternating  cycles  of  hydrostatic
pressure between ambient and ultra-high levels i.e., 20,000 psi or greater to safely, conveniently and reproducibly control the actions of molecules
in biological samples, such as cells and tissues from human, animal, plant and microbial sources.

PCT is an enabling platform technology based on a physical process that had not previously been used to control bio-molecular interactions.
PCT  uses  internally  developed  instrumentation  that  is  capable  of  cycling  pressure  between  ambient  and  ultra-high  levels  at  controlled
temperatures and specific time intervals, to rapidly and repeatedly control the interactions of bio-molecules, such as proteins, DNA, RNA, lipids
and small molecules. Our laboratory instrument family, the Barocycler®, and our internally developed consumables product line, which include
our  unique  MicroTubes,  MicroCaps,  MicroPestles,  BaroFlex  and  PULSE®  (Pressure  Used  to  Lyse  Samples  for  Extraction)  Tubes,  and
application specific kits (containing consumable products and reagents), together make up our PCT SPS.

In 2015, together with an investment bank, we formed a subsidiary called Pressure BioSciences Europe (“PBI Europe”) in Poland. We have
49% ownership interest with the investment bank retaining 51%. PBI Europe has never had any operating activities and we cannot reasonably
predict  when  operations  will  commence.  Therefore,  we  don’t  have  control  of  the  subsidiary  and  did  not  consolidate  them  in  our  financial
statements.

Patents

PBI  has  14  United  States  granted  patents  and  one  foreign  granted  patent  (Japan:  5587770,  EXTRACTION  AND  PARTITIONING  OF
MOLECULES) covering multiple applications of PCT in the life sciences field. PBI also has 19 pending patents in the USA, Canada, Europe,
Australia,  China,  and  Taiwan  PCT  employs  a  unique  approach  that  we  believe  has  the  potential  for  broad  use  in  a  number  of  established  and
emerging life sciences areas, which include, but are not limited to:

● biological sample  preparation  –  including  but  not  limited  to  sample  extraction,  homogenization,  and  digestion  -  in  such  study  areas as

genomic, proteomic, lipidomic, metabolomic and small molecule;

● pathogen inactivation;

● protein purification;

● control of chemical reactions, particularly enzymatic; and

● immunodiagnostics.

We are also the exclusive distributor, throughout the Americas for Constant System’s cell disruption equipment, parts, and consumables. CS,
a  British  company  located  several  hours  northwest  of  London,  England,  has  been  providing  niche  biomedical  equipment,  related  consumable
products,  and  services  to  a  global  client  base  since  1989.  CS  designs,  develops,  and  manufactures  high  pressure  cell  disruption  equipment
required  by  life  sciences  laboratories  worldwide,  particularly  disruption  systems  for  the  extraction  of  proteins.  The  CS  equipment  provides  a
constant and controlled cell disruptive environment, giving the user superior, constant, and reproducible results whatever the application. CS has
over 900 units installed in over 40 countries worldwide. The CS cell disruption equipment has proven performance in the extraction of cellular
components, such as protein from yeast, bacteria, mammalian cells, and other sample types.

- 34 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
The  CS  pressure-based  cell  disruption  equipment  and  our  PCT-based  instrumentation  complement  each  other  in  several  important  ways.
While both the CS and our technologies are based on high pressure, each product line has fundamental scientific capabilities that the other does
not  offer.  Our  PCT  Platform  uses  certain  patented  pressure  mechanisms  to  achieve  small-scale,  molecular  level  effects.  CS’s  technology  uses
different, proprietary pressure mechanisms for larger-scale, non-molecular level processing. In a number of routine laboratory applications, such
as  protein  extraction,  both  effects  can  be  critical  to  success.  Therefore,  for  protein  extraction  and  a  number  of  other  important  scientific
applications, we believe laboratories will benefit by using the CS and our products, either separately or together.

Primary Fields of Use and Application for PCT

Sample preparation is widely regarded as a significant impediment to research and discovery and sample extraction is generally regarded as
one  of  the  key  parts  of  sample  preparation.  The  process  of  preparing  samples  for  genomic,  proteomic,  lipidomic,  and  small  molecule  studies
includes a crucial step called sample extraction or sample disruption. This is the process of extracting biomolecules such as nucleic acid i.e., DNA
and/or RNA, proteins, lipids, or small molecules from the plant or animal cells and tissues that are being studied. Our current commercialization
efforts  are  based  upon  our  belief  that  pressure  cycling  technology  provides  a  superior  solution  for  sample  extraction  when  compared  to  other
available technologies or procedures and thus might significantly improve the quality of sample preparation, and thus the quality of the test result.

Within  the  broad  field  of  biological  sample  preparation,  in  particular  sample  extraction,  we  focus  the  majority  of  our  PCT  and  constant
pressure  (“CP”)  product  development  efforts  in  three  specific  areas:  biomarker  discovery  (primarily  through  mass  spectrometric  analysis),
forensics, and histology. We believe that our existing PCT and CP-based instrumentation and related consumable products fill an important and
growing need in the sample preparation market for the safe, rapid, versatile, reproducible and quality extraction of nucleic acids, proteins, lipids,
and small molecules from a wide variety of plant, animal, and microbiological cells and tissues.

Biomarker Discovery - Mass Spectrometry

A biomarker is any substance (e.g., protein, DNA) that can be used as an indicator of the presence or absence of a particular disease-state or
condition,  and/or  to  measure  the  progression  and  effects  of  therapy.  Biomarkers  can  help  in  the  diagnosis,  prognosis,  therapy,  prevention,
surveillance, control, and cure of diseases and medical conditions.

A mass spectrometer is a laboratory instrument used in the analysis of biological samples, often focused on proteins, in life sciences research.
It  is  frequently  used  to  help  discover  biomarkers.  According  to  a  recently  published  market  report  by  Transparency  Market  Research,
“Spectrometry Market (Atomic, Molecular and Mass Spectrometry) - Global Scenario, Trends, Industry Analysis, Size, Share & Forecast 2011 –
2017,” the global spectrometry market was worth $10.2 billion in 2011 and is expected to reach $15.2 billion in 2017, growing at a compound
annual growth rate of 6.9% from 2011 to 2017. In the overall global market, the North American market is expected to maintain its lead position
in  terms  of  revenue  until  2017  and  is  expected  to  have  approximately  36.2%  of  the  market  revenue  share  in  2017,  followed  by  Europe.  We
believe PCT and CP-based products offer significant advantages in speed and quality compared with current techniques used in the preparation of
samples for mass spectrometry analysis.

- 35 -

 
 
 
 
 
 
 
 
 
Forensics

The detection of DNA has become a part of the analysis of forensic samples by laboratories and criminal justice agencies worldwide in their
efforts to identify the perpetrators of violent crimes and missing persons. Scientists from the University of North Texas and Florida International
University have reported improvements in DNA yield from forensic samples (e.g., bone and hair) when using the PCT platform in the sample
preparation process. We believe that PCT may be capable of differentially extracting DNA from sperm cells and female epithelial cells captured
in swabs collected from rape victims and subsequently stored in rape kits. We also believe that there are many completed rape kits that remain
untested for reasons such as cost, time and quality of results. We further believe that the ability to differentially extract DNA from sperm and not
epithelial cells could reduce the cost of such testing, while increasing the quality, safety and speed of the testing process.

Histology

The most commonly used technique worldwide for the preservation of cancer and other tissues for subsequent pathology evaluation is process
them  into  formalin-fixed,  paraffin-embedded  (“FFPE”)  tissue  samples.  We  believe  that  the  quality  and  analysis  of  FFPE  tissues  is  highly
problematic, and that PCT offers significant advantages over current processing methods, including standardization, speed, biomolecule recovery,
and safety.

Our customers include researchers at academic laboratories, government agencies, biotechnology companies, pharmaceutical firms, and other
life science institutions in the North, Central, and South America; Europe, and Asia. Our goal is to continue aggressive market penetration in these
target  groups.  We  also  believe  that  there  is  a  significant  opportunity  to  sell  and/or  lease  additional  Barocycler®  instrumentation  to  additional
laboratories at current customer institutions.

If  we  are  successful  in  commercializing  PCT  in  applications  beyond  our  current  focus  area  of  genomic,  proteomic,  lipidomic,  and  small
molecule sample preparation, and if we are successful in our attempts to attract additional capital, our potential customer base could expand to
include  hospitals,  reference  laboratories,  pharmaceutical  manufacturing  plants  and  other  sites  involved  in  each  specific  application.  If  we  are
successful in forensics, our potential customers could be forensic laboratories, military and other government agencies. If we are successful in
histology (extraction of biomolecules from FFPE tissues), our potential customers could be pharmaceutical companies, hospitals, and laboratories
focused on drug discovery or correlation of disease states.

Going Concern

We have experienced negative cash flows from operations with respect to our pressure cycling technology business since our inception. As of
December 31, 2017, we did not have adequate working capital resources to satisfy our current liabilities and as a result we have substantial doubt
about our ability to continue as a going concern. Based on our current projections, including equity financing subsequent to December 31, 2017,
we believe we will have the cash resources that will enable us to continue to fund normal operations into the foreseeable future.

The audit report issued by our independent registered public accounting firm on our audited consolidated financial statements for the fiscal
year ended December 31, 2017, contains an explanatory paragraph regarding our ability to continue as a going concern. The audit report issued by
our  independent  registered  public  accounting  firm  for  our  financial  statements  for  the  fiscal  year  ended  December  31,  2017  states  that  our
auditing firm has substantial doubt in our ability to continue as a going concern due to the risk that we may not have sufficient cash and liquid
assets to cover our operating and capital requirements for the next twelve-month period; and, if sufficient cash cannot be obtained, we would have
to substantially alter, or possibly even discontinue, operations. The accompanying financial statements do not include any adjustments that might
result from the outcome of this uncertainty.

The conditions described above could adversely affect our ability to obtain additional financing on favorable terms, if at all, and may cause
investors  to  have  reservations  about  our  long-term  prospects,  and  may  adversely  affect  our  relationships  with  customers.  There  can  be  no
assurance  that  our  auditing  firm  will  not  issue  the  same  opinion  in  the  future.  If  we  cannot  successfully  continue  as  a  going  concern,  our
stockholders may lose their entire investment in us.

- 36 -

 
 
 
 
 
 
 
 
 
 
 
 
RESULTS OF OPERATIONS

Year Ended December 31, 2017 as compared with December 31, 2016

Revenue

We had total revenue of $2,240,498, in the year ended December 31, 2017 as compared with $1,976,487 in the prior year, a 13% increase.

The increase was due to product sales growth.

Products,  Services,  and  Other.  Revenue  from  the  sale  of  products  and  services  was  $2,065,891  in  the  year  ended  December  31,  2017
compared with $1,794,749 in the year ended December 31, 2016, a 15% increase. Revenue included sales of both PBI and CS’s pressure-based
products. Sales of instrumentation increased in 2017 by $253,806  or  21%,  from  $1,205,520  for  FY  2016  to  $1,459,326  for  FY  2017.  Sales  of
consumables were $260,331 for the year ended December 31, 2017 compared to $199,873 for the same period in 2016, an increase of $60,458 or
30%. Products, Services, and Other Revenue included $77,088 from non-cash transactions in the current year while the prior year included non-
cash transactions of $63,956. Revenue from non-cash transactions was recognized on the fair value of the assets involved per ASC 845.

Grant Revenue. During 2017, we recorded $174,607 of grant revenue as compared with $181,738 in 2016. In December 2014, the Company
was  awarded  a  $1,020,969  SBIR  Phase  II  grant  (2R44HG007136)  from  the  National  Human  Genome  Research  Institute  of  the  NIH.  Entitled
“High Pressure Sample Preparation Instrumentation for DNA Sequencing”, this grant is helping to fund the development of an automated, high-
throughput, high pressure system (instrument and consumables) to enable significantly better control of DNA fragmentation - a critical step in the
preparation of samples for Next Generation Sequencing platforms. This system will be based on significant technological advancements over the
classic  hydrodynamic  DNA  shearing  approach  that  has  been  successfully  and  widely  used  in  the  field  of  DNA  sequencing  for  many  years.  In
March 2018, we received an extension on the SBIR Phase II grant to utilize unused funds until November 30, 2018.

Cost of Products and Services

The cost of products and services was $1,273,354 for the year ended December 31, 2017, compared with $834,012 in 2016. Our overall gross
profit margin was 43% for FY 2017 vs. 58% for FY 2016. The prior year margin was affected by a $136,000 credit relating to the SBIR Phase II
grant.  Excluding  this  credit,  our  prior  year  margin  would  have  been  51%.  The  current  year  cost  of  products  and  services  includes  a  $159,600
inventory allowance for the older generation of Barocycler instruments held in stock, the NEP3229.

Research and Development

Research  and  development  expenditures  were  $988,597  for  2017  compared  to  $1,183,011  in  2016,  a  decrease  of  $194,414  or  16%.  This
decrease  resulted  primarily  from  the  allocation  of  R&D  resources  to  production  of  our  instruments.  The  prior  year  expenses  included  charges
incurred with a collaborator. Research and development expense also included $92,055 and $65,500 of non-cash, stock-based compensation in
2017 and 2016, respectively.

Selling and Marketing

Selling and marketing expenses were $1,209,334 in 2017 compared to $872,365 in 2016, an increase of $336,969, or 39%. This increase is
primarily  attributed  to  an  increase  in  employee  staffing  resulting  from  the  development  of  a  field  sales  force.  Selling  and  marketing  expense
included $54,404 and $42,314 of non-cash stock based compensation expense in 2017 and 2016, respectively.

General and Administrative

General and administrative costs were $3,416,261 in the year ended December 31, 2017, as compared with $2,822,752 in 2016, an increase of
$593,509 or 21%. The prior year costs included credits received from charges incurred with a former professional service provider offset by the
hire of a CFO in 2017. During the years ended December 31, 2017 and 2016, general and administrative expense included $259,968 and $272,150
of non-cash, stock-based compensation expense, respectively.

- 37 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Operating Loss

Our  operating  loss  was  $4,647,048  for  the  year  ended  December  31,  2017  as  compared  to  $3,735,653  for  the  prior  year,  an  increase  of
$911,395 or 24%. This increase in operating loss was due primarily to the increase in Sales and Marketing expenses, the inventory allowance of
$159,600 and the one-time administrative credits in the prior year off-set to a certain extent by an increase in total revenue.

Other income (expense), net

Interest  Expense. Net  interest  expense  totaled  $6,055,420  for  the  year  ended  December  31,  2017  as  compared  to  interest  expense  of
$4,501,186 for the year ended December 31, 2016. The increase was from interest on additional short-term loans, drawdowns on our revolving
note  and  interest  accruals  at  certain  trigger  dates  during  the  revolving  note’s  term.  In  connection  with  loans  issued  in  2015  and  2016,  we  are
amortizing deferred financing costs and imputed interest against the debt discount on loans.

Other income (expense) net

We  recognized  $5,674  in  expense  during  2017,  compared  to  $1,112  of  expense  in  2016.  Other  expenses  include  foreign  exchange  losses

relating to overseas purchases in local currency.

Impairment loss on investment

The value of our investment in common stock of Everest Investments Holdings S.A. (“Everest”) has declined since the date of receipt of the
stock  in  2015.  We  evaluated  the  decline  and  considered  it  as  an  “other  than  temporary  impairment”  reduction.  Thus,  the  impairment  loss  was
recognized  as  a  charge  in  the  consolidated  statements  of  operations.  During  2017  and  2016,  we  recorded  impairment  losses  of  $6,069  and
$373,682 respectively, which represented the reduction in value of these securities.

Gain on extinguishment of debt

In  connection  with  payments  of  interest  in  common  stock,  we  calculated  gains  of  $218,452  on  the  difference  between  the  stock’s  trading
price on date of issuance and the interest payable in cash. The gains were offset by fees paid to extend the terms on short-term loans. The current
year gains were offset by $33,000 loan extension fees recorded as losses on debt modifications.

Change in fair value of derivative liabilities

During  the  year  ended  December  31,  2016,  we  recorded  non-cash  income  of  $5,904,649  from  warrant  and  conversion  option  liability
revaluations in our consolidated statements of operations due to a decrease in the fair value of the derivative warrants and the conversion option
liabilities on our debt. This decrease in fair value was primarily due to a decrease in the price per share of our common stock. We early adopted
ASU 2017-11 and applied the guidance to derivative accounting. Therefore, we reclassified the warrant and conversion option liabilities to equity
and stopped fair valuing the instruments in 2017.

Income Taxes

We did not record an income tax benefit or provision for the years ended December 31, 2017 or 2016.

Net Loss

During  the  year  ended  December  31,  2017,  we  recorded  a  net  loss  of  $10,715,561  or  $(9.62)  per  share,  as  compared  with  $2,706,984  or
$(2.97) per share during the year ended December 31, 2016. This increase in net loss is primarily attributable to the discontinued changes in fair
value of our derivative liabilities, the increase in Sales and Marketing expenses, the one-time administrative credits in the prior year and additional
interest of $1.5 million from current year borrowings.

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LIQUIDITY AND FINANCIAL CONDITION

As of December 31, 2017, we did not have adequate working capital resources to satisfy our current liabilities. We have been successful in
raising cash through debt and equity offerings in the past. We issued a promissory note in the aggregate principal amount of up to $4,000,000 in
October 2016 as amended in February 2018 that we can draw funds from, and, through December 31, 2017, we have drawn down $3.5 million
($500,000 subsequent to December 31, 2017). We have efforts in place to continue to raise cash through debt and equity offerings.

We believe our current and projected capital raising plans, and our projected continued increases in revenue, will enable us to extend our cash
resources for the foreseeable future. Although we have successfully completed equity and debt financings and reduced expenses in the past, we
cannot assure you that our plans to address these matters in the future will be successful.

We believe we will need approximately $15 million in additional capital to fund our three-pronged operational plan, which was designed to

help increase revenues and reach profitability, by:

A.

implementing a next-generation upgrade to our product line and offering a superior instrument with greater net margins;

B. gaining additional non-dilutive monies from governmental research and development applications, and/or engineering projects; and

C.

retaining a  small  team  of  sales  and  marketing  persons  to  target  research  facilities  and  academic  institutions,  and  cultivate  our  current
customer list of pharmaceutical, military and paramilitary organizations.

However, if we are unable to obtain such funds through sales, the capital markets or other source of financing on acceptable terms, or at all,
we will likely be required to cease our operations, pursue a plan to sell our operating assets, or otherwise modify our business strategy, which
could materially harm our future business prospects. These conditions raise substantive doubt about our ability to continue as a going concern.

Net cash used in operating activities was $3,904,549 for the year ended December 31, 2017 as compared with $3,805,851 for the year ended

December 31, 2016.

Net cash used in investing activities for the year ended December 31, 2017 totaled $171,825 compared to $7,203 in the prior period. Cash

capital expenditures included BaroFold patents, laboratory equipment and IT equipment.

Net cash provided by financing activities for the year ended December 31, 2017 was $4,019,044 as compared with $3,834,634 in the prior

year.

In 2017,

A $1,755,850 in aggregate net proceeds were raised from sales of convertible debentures and $925,541 payments were made for convertible

debt.

B Loans in  the  aggregate  amount  of  $2,905,752  were  received  during  the  year  and  we  made  payments  on  new  and  existing  debt  of

$1,894,231.

C  $2,070,000 in aggregate net proceeds were drawn down from a revolving note facility.

D $140,214 net proceeds were received from warrant exercises.

Our common stock is currently traded on the OTCQB tier of the OTC Markets under the trading symbol “PBIO.”

- 39 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
COMMITMENTS AND CONTINGENCIES

Royalty Commitments

In 1996, we acquired our initial equity interest in BioSeq, Incorporated (“BioSeq”). At the time, BioSeq was developing our original pressure
cycling  technology.  They  acquired  its  pressure  cycling  technology  from  BioMolecular Assays,  Inc.  (“ BMA”)  under  a  technology  transfer  and
patent assignment agreement. In 1998, we purchased all of the remaining, outstanding capital stock of BioSeq; and, consequently, the technology
transfer  and  patent  assignment  agreement  was  amended  to  require  us  to  pay  BMA  a  5%  royalty  on  our  sales  of  products  or  services  that
incorporate or utilize the original pressure cycling technology that BioSeq acquired from BMA. Similarly, the Company is required to pay BMA
5% of the proceeds from any sale, transfer or license of all or any portion of the original pressure cycling technology. These payment obligations
terminated March 7, 2016. During the year ended December 31, 2016, we incurred approximately $6,963 in royalty expense associated with our
obligation to BMA.

In connection with our acquisition of BioSeq, we licensed certain limited rights to the original pressure cycling technology back to BMA.
This license is non-exclusive and limits the use of the original pressure cycling technology by BMA solely for molecular applications in scientific
research and development, and in scientific plant research and development. BMA is required to pay us a royalty equal to 20% of any license or
other fees and royalties, but not including research support and similar payments, it receives in connection with any sale, assignment, license or
other transfer of any rights granted to BMA under the license. BMA was required to pay us these royalties until the expiration of the patents held
by BioSeq in March 2016. We have not received any royalty payments from BMA under this license.

Battelle Memorial Institute

In  December  2008,  we  entered  into  an  exclusive  patent  license  agreement  with  the  Battelle  Memorial  Institute  (“Battelle”).  The  licensed
technology  is  described  in  the  patent  application  filed  by  Battelle  on  July  31,  2008  (US  serial  number  12/183,219).  This  application  includes
subject matter related to a method and a system for improving the analysis of protein samples including, through an automated system, utilizing
pressure and a pre-selected agent to obtain a digested sample in a significantly shorter period of time than current methods, while maintaining the
integrity of the sample throughout the preparatory process. Pursuant to the terms of the agreement, we paid Battelle a non-refundable initial fee of
$35,000. In addition to royalty payments on net sales on “licensed products,” we are obligated to make minimum royalty payments for each year
we retain the rights outlined in the patent license agreement; and, we are required to have our first commercial sale of the licensed products within
one  year  following  the  issuance  of  the  patent  covered  by  the  licensed  technology. After  re-negotiating  the  terms  of  the  contract  in  2013,  the
minimum annual royalty was $1,200 in 2014 and $2,000 in 2015; the minimum royalties are $3,000 in 2016, $4,000 in 2017 and $5,000 in 2018
and each calendar year thereafter during the term of the agreement.

Target Discovery Inc.

In March 2010, we signed a strategic product licensing, manufacturing, co-marketing, and collaborative research and development agreement
with Target Discovery Inc. (“TDI”), a related party. Under the terms of the agreement, we have been licensed by TDI to manufacture and sell a
highly innovative line of chemicals used in the preparation of tissues for scientific analysis (“TDI reagents”). The TDI reagents were designed for
use in combination with our pressure cycling technology. The respective companies believe that the combination of PCT and the TDI reagents
can fill an existing need in life science research for an automated method for rapid extraction and recovery of intact, functional proteins associated
with cell membranes in tissue samples. We did not incur any royalty obligation under this agreement in 2017 or 2016. We executed an amendment
to  this  agreement  on  October  1,  2016  wherein  we  agreed  to  pay  a  monthly  fee  of  $1,400  for  the  use  of  a  lab  bench,  shared  space  and  other
utilities, and $2,000 per day for technical support services as needed. Mr. Jeffrey N. Peterson, the chief executive officer of TDI, has served as a
director of the Company since July 2011 and as Chairman of the Board starting in 2012.

- 40 -

 
 
 
 
 
 
 
 
 
 
Severance and Change of Control Agreements

Each of Mr. Schumacher, Dr. Ting, Dr. Lazarev, and Dr. Lawrence, executive officers of the Company, are entitled to receive a severance
payment if terminated by us without cause. The severance benefits would include a payment in an amount equal to one year of such executive
officer’s annualized base salary compensation plus accrued paid time off. Additionally, the officer will be entitled to receive medical and dental
insurance coverage for one year following the date of termination.

Pursuant to severance agreements with each of Mr. Schumacher, Dr. Ting, Dr. Lazarev and Dr. Lawrence, each such executive officers, is
entitled  to  receive  a  change  of  control  payment  in  an  amount  equal  to  one  year  (other  than  Mr.  Schumacher)  of  such  executive  officer’s
annualized base salary compensation, accrued paid time off, and medical and dental coverage, in the event of a change of control of our Company.
In the case of Mr. Schumacher, his payment is equal to two years of annualized base salary compensation, accrued paid time off, and two years of
medical and dental coverage.

Pursuant to our equity incentive plans, any unvested stock options held by a named executive officer will become fully vested upon a change

in control (as defined in the 2005 Equity Incentive Plan) of our Company.

Lease Commitments

We  lease  building  space  under  non-cancelable  leases  in  South  Easton,  MA  and  lab  space  in  Medford,  MA.  Rental  costs  are  expensed  as
incurred. During 2017 and 2016 we incurred $140,783 and $125,819, respectively, in rent expense for the use of our corporate office and research
and development facilities.

Following is a schedule by years of future minimum rental payments required under operating leases with initial or remaining non-cancelable

lease terms in excess of one year as of December 31, 2017:

2018
2019
2020
2021
Thereafter

  $

  $

166,353 
82,953 
82,953 
- 
- 
332,259 

Off-Balance Sheet Arrangements

We do not have any off-balance sheet arrangements as of December 31, 2017 and December 31, 2016.

CRITICAL ACCOUNTING POLICIES AND ESTIMATES

Principles of Consolidation

The consolidated financial statements include the accounts of Pressure BioSciences, Inc., and its wholly-owned subsidiary PBI BioSeq, Inc.

All intercompany accounts and transactions have been eliminated in consolidation.

Use of Estimates

To  prepare  our  consolidated  financial  statements  in  conformity  with  accounting  principles  generally  accepted  in  the  United  States  of
America, we are required to make significant estimates and assumptions that affect the reported amounts of assets and liabilities and disclosure of
contingent assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the reporting
period.  In  addition,  significant  estimates  were  made  in  projecting  future  cash  flows  to  quantify  deferred  tax  assets,  the  costs  associated  with
fulfilling our warranty obligations for the instruments that we sell, and the estimates employed in our calculation of fair value of stock options
awarded and warrant derivative liability. We base our estimates on historical experience and on various other assumptions that we believe to be
reasonable under the circumstances, the results of which form the basis for making judgments about the carrying values of assets and liabilities
that are not readily apparent from other sources. Actual results could differ from the estimates and assumptions used.

- 41 -

 
 
 
 
 
 
 
 
 
   
   
   
   
 
 
 
 
 
 
 
 
 
Revenue Recognition

We recognize revenue in accordance with FASB ASC 605,  Revenue Recognition. Revenue is recognized when realized or when realizable
and earned when all the following criteria have been met: persuasive evidence of an arrangement exists; goods were shipped, delivery of service
has occurred and risk of loss has passed to the customer; the seller’s price to the buyer is fixed or determinable; and collectability is reasonably
assured.

Our current Barocycler® instruments require a basic level of instrumentation expertise to set-up for initial operation. To support a favorable
first  experience  for  our  customers,  upon  customer  request,  and  for  an  additional  fee,  will  send  a  highly  trained  technical  representative  to  the
customer site to install Barocycler®s that we sell, lease, or rent through our domestic sales force. The installation process includes uncrating and
setting up the instrument, followed by introductory user training. Product revenue related to current Barocycler® instrumentation and Constant
Systems  products  is  recognized  upon  shipment  of  the  unit.  In  the  case  where  the  customer  requests  installation  and  training,  the  additional
revenue  related  to  the  installation  and  training  is  recognized  upon  the  completion  of  the  installation  and  introductory  training  process  of  the
instrumentation at the customer location. Product revenue related to sales of PCT instrumentation to our foreign distributors is recognized upon
shipment  through  a  common  carrier.  We  provide  for  the  expected  costs  of  warranty  upon  the  recognition  of  revenue  for  the  sales  of  our
instrumentation. Our sales arrangements do not provide our customers with a right of return. Product revenue related to our consumable products
such  as  PULSE®  Tubes,  MicroTubes,  and  application  specific  kits  is  recorded  upon  shipment  through  a  common  carrier.  Shipping  costs  are
included in sales and marketing expense. Any shipping costs billed to customers are recognized as revenue.

We apply ASC 845, “Accounting for Non-Monetary Transactions”, to account for products and services sold through non-cash transactions
based on the fair values of the products and services involved, where such values can be determined. Non-cash exchanges would require revenue
to be recognized at recorded cost or carrying value of the assets or services sold if any of the following conditions apply:

a) The fair value of the asset or service involved is not determinable.

b) The transaction is an exchange of a product or property held for sale in the ordinary course of business for a product or property to be sold

in the same line of business to facilitate sales to customers other than the parties to the exchange.

c) The transaction lacks commercial substance.

  We currently record revenue for its non-cash transactions at recorded cost or carrying value of the assets or services sold.

In accordance with FASB ASC 840,  Leases, we account for our lease agreements under the operating method. We record revenue over the
life  of  the  lease  term  and  we  record  depreciation  expense  on  a  straight-line  basis  over  the  thirty-six  month  estimated  useful  life  of  the
Barocycler®  instrument.  The  depreciation  expense  associated  with  assets  under  lease  agreement  is  included  in  the  “Cost  of  PCT  products  and
services”  line  item  in  our  accompanying  consolidated  statements  of  operations.  Many  of  our  lease  and  rental  agreements  allow  the  lessee  to
purchase  the  instrument  at  any  point  during  the  term  of  the  agreement  with  partial  or  full  credit  for  payments  previously  made.  We  pay  all
maintenance costs associated with the instrument during the term of the leases.

Revenue from government grants is recorded when expenses are incurred under the grant in accordance with the terms of the grant award.

Revenue from the sale of CS’s cell disruption equipment, parts, and consumables is recognized when products are shipped.

- 42 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Deferred  revenue  represents  amounts  received  from  grants  and  service  contracts  for  which  the  related  revenues  have  not  been  recognized
because one or more of the revenue recognition criteria have not been met. Revenue from service contracts is recorded ratably over the length of
the contract.

Our  transactions  sometimes  involve  multiple  elements  i.e.,  products  and  services.  Revenue  under  multiple  element  arrangements  is
recognized  in  accordance  with  FASB ASC  605-25  Multiple-Element Arrangements (“ASC 605”).  When  vendor  specific  objective  evidence  or
third party evidence of selling price for deliverables in an arrangement cannot be determined, we Company develop a best estimate of the selling
price  to  separate  deliverables,  and  allocates  arrangement  consideration  using  the  relative  selling  price  method.  Additionally,  this  guidance
eliminates  the  residual  method  of  allocation.  If  an  arrangement  includes  undelivered  elements  that  are  not  essential  to  the  functionality  of  the
delivered elements, we defer the fair value of the undelivered elements with the residual revenue allocated to the delivered elements. Fair value is
determined  based  upon  the  price  charged  when  the  element  is  sold  separately.  If  there  is  not  sufficient  evidence  of  the  fair  value  of  the
undelivered  elements,  no  revenue  is  allocated  to  the  delivered  elements  and  the  total  consideration  received  is  deferred  until  delivery  of  those
elements for which objective and reliable evidence of the fair value is not available. We provide certain customers with extended service contracts
with revenue recognized ratably over the life of the contract.

Intangible Assets

We  have  classified  as  intangible  assets,  costs  associated  with  the  fair  value  of  acquired  intellectual  property.  Intangible  assets,  including
patents,  are  being  amortized  on  a  straight-line  basis  over  sixteen  years.  We  perform  an  annual  review  of  our  intangible  assets  for  impairment.
When  impairment  is  indicated,  any  excess  of  carrying  value  over  fair  value  is  recorded  as  a  loss. As  of  December  31,  2017  and  2016,  the
outstanding balance for intangible assets was $750,000 and zero, respectively.

Long-Lived Assets

The  Company’s  long-lived  assets  are  reviewed  for  impairment  in  accordance  with  the  guidance  of  the  FASB ASC  360-10-05,  Property,
Plant,  and  Equipment,  whenever  events  or  changes  in  circumstances  indicate  that  the  carrying  amount  of  the  asset  may  not  be  recoverable.
Recoverability of an asset to be held and used is measured by a comparison of the carrying amount of an asset to the future undiscounted cash
flows  expected  to  be  generated  by  the  asset.  If  such  asset  is  considered  to  be  impaired,  the  impairment  to  be  recognized  is  measured  by  the
amount  by  which  the  carrying  amount  of  the  asset  exceeds  its  fair  value.  Through  December  31,  2017,  the  Company  had  not  experienced
impairment  losses  on  its  long-lived  assets.  While  our  current  and  historical  operating  losses  and  cash  flow  are  indicators  of  impairment,  we
performed an impairment test at December 31, 2017 and determined that such long-lived assets were not impaired.

Warrant Derivative Liability

The warrants issued in November 2011 in connection with the registered direct offering of Series D Convertible Preferred Stock (the “Series
D  Warrants”)  and  the  warrants  issued  in  2015  and  2016  in  connection  with  the  $6.3  million  PIPE  convertible  debentures  (the  “Debenture
Warrants”)  are  measured  at  fair  value  and  liability-classified  because  the  Series  D  Warrants  and  Debenture  Warrants  contained  “down-round
protection” and therefore, did not meet the scope exception for treatment as a derivative under ASC 815, Derivatives and Hedging. Since “down-
round  protection”  is  not  an  input  into  the  calculation  of  the  fair  value  of  the  warrants,  the  warrants  cannot  be  considered  indexed  to  the
Company’s own stock which is a requirement for the scope exception as outlined under ASC 815. The estimated fair value of the warrants was
determined using the binomial model, resulting in an allocation of the gross proceeds of $283,725 to the warrants issued in the Series D registered
direct offering.

In connection with the sale of convertible debentures in 2015 and 2016, the estimated fair value of the warrants was determined using the
binomial model, resulting in an allocation of the gross proceeds of $2,847,624 to the warrants issued with convertible debentures. The fair value
will be affected by changes in inputs to that model including our stock price, expected stock price volatility, the contractual term, and the risk-free
interest  rate.  We  early  adopted ASU  2017-11  and  applied  the  guidance  to  derivative  accounting.  Therefore,  we  reclassified  the  warrant  and
conversion option liabilities to equity and stopped fair valuing the instruments in 2017.

- 43 -

 
 
 
 
 
 
 
 
 
 
 
Conversion Option Liability

We have signed convertible notes and have determined that conversion options are embedded in the notes and it is required to bifurcate the
conversion option from the host contract under ASC 815 and account for the derivatives at fair value. The estimated fair value of the conversion
options  was  determined  using  the  binomial  model.  The  fair  value  of  the  conversion  options  will  be  classified  as  a  liability  until  the  debt  is
converted by the note holders or paid back by the Company. The fair value will be affected by changes in inputs to that model including our stock
price, expected stock price volatility, the contractual term, and the risk-free interest rate. We early adopted ASU 2017-11 and applied the guidance
to  derivative  accounting.  Therefore,  we  reclassified  the  warrant  and  conversion  option  liabilities  to  equity  and  stopped  fair  valuing  the
instruments in 2017.

Accounts Receivable and Allowance for Doubtful Accounts

We maintain allowances for estimated losses resulting from the inability of our customers to make required payments. Judgments are used in
determining the allowance for doubtful accounts and are based on a combination of factors. Such factors include historical collection experience,
credit policy and specific customer collection issues. In circumstances where we are aware of a specific customer’s inability to meet its financial
obligations to us (e.g., due to a bankruptcy filing), we record a specific reserve for bad debts against amounts due to reduce the net recognized
receivable  to  the  amount  we  reasonably  believe  will  be  collected.  We  perform  ongoing  credit  evaluations  of  our  customers  and  continuously
monitor collections and payments from our customers. While actual bad debts have historically been within our expectations and the provisions
established, we cannot guarantee that we will continue to experience the same bad debt rates that we have in the past. A significant change in the
liquidity or financial position of any of our customers could result in the uncollectability of the related accounts receivable and could adversely
impact our operating cash flows in that period.

Inventories

Inventories are valued at the lower of cost (average cost) or market (sales price). The cost of Barocyclers consists of the cost charged by the
contract  manufacturer.  The  cost  of  manufactured  goods  includes  material,  freight-in,  direct  labor,  and  applicable  overhead.  In  assessing  the
ultimate realization of inventories, management judgment is required to determine the reserve for obsolete or excess inventory. Inventory on hand
may exceed future demand either because the product is obsolete, or because the amount on hand is more than can be used to meet future needs.
We provide for the total value of inventories that we determine to be obsolete or excess based on criteria such as customer demand and changing
technologies. We historically have not experienced significant inaccuracies in computing our reserves for obsolete or excess inventory.

Equity Transactions

We evaluate the proper classification of our equity instruments that embody an unconditional obligation requiring the issuer to redeem it by
transferring assets at a determinable date or that contain certain conditional obligations, typically classified as equity, be classified as a liability.
We  record  amortized  financing  costs  associated  with  our  capital  raising  efforts  in  our  consolidated  statements  of  operations.  These  include
amortization  of  debt  issue  costs  such  as  cash,  common  stock  and  warrants  and  other  securities  issued  to  finders  and  placement  agents,  and
amortization of debt discount created by in-the-money conversion features on convertible debt and allocates the proceeds amongst the securities
based  on  relative  fair  values.  We  based  our  estimates  and  assumptions  on  the  best  information  available  at  the  time  of  valuation;  however,
changes in these estimates and assumptions could have a material effect on the valuation of the underlying instruments.

Stock-Based Compensation

We  account  for  employee  and  non-employee  director  stock-based  compensation  using  the  fair  value  method  of  accounting.  Compensation
cost  arising  from  stock  options  to  employees  and  non-employee  directors  is  recognized  using  the  straight-line  method  over  the  vesting  period,
which represents the requisite service or performance period. The calculation of stock-based compensation requires us to estimate several factors,
most  notably  the  term,  volatility  and  forfeitures.  We  estimate  the  option  term  using  historical  terms  and  estimate  volatility  based  on  historical
volatility of our common stock over the option’s expected term. Expected forfeitures based on historical forfeitures are used in calculating the
expense  related  to  stock-based  compensation  associated  with  stock  awards.  Our  estimates  and  assumptions  are  based  on  the  best  information
available  at  the  time  of  valuation;  however,  changes  in  these  estimates  and  assumptions  could  have  a  material  effect  on  the  valuation  of  the
underlying instruments.

- 44 -

 
 
 
 
 
 
 
 
 
 
 
 
Recent Accounting Standards

From time to time, new accounting pronouncements are issued by the FASB or other standard setting bodies and adopted by the Company as
of  the  specified  effective  date.  Unless  otherwise  discussed,  the  Company  believes  that  the  impact  of  recently  issued  standards  that  are  not  yet
effective will not have a material impact on its financial position or results of operations upon adoption.

In  February  2016,  the  FASB  issued ASU  2016-02,  Leases  (Topic  842).  The  new  standard  requires  the  recognition  of  assets  and  liabilities
arising from lease transactions on the balance sheet and the disclosure of key information about leasing arrangements. Accordingly, a lessee will
recognize  a  lease  asset  for  its  right  to  use  the  underlying  asset  and  a  lease  liability  for  the  corresponding  lease  obligation.  Both  the  asset  and
liability  will  initially  be  measured  at  the  present  value  of  the  future  minimum  lease  payments  over  the  lease  term.  Subsequent  measurement,
including the presentation of expenses and cash flows, will depend on the classification of the lease as either finance or an operating lease. Initial
costs directly attributable to negotiating and arranging the lease will be included in the asset. Lessees will also be required to provide additional
qualitative  and  quantitative  disclosures  regarding  the  amount,  timing  and  uncertainty  of  cash  flows  arising  from  leases.  The  new  standard  is
effective for fiscal years beginning after December 15, 2018, and interim periods therein. Early adoption is permitted. We are currently evaluating
the impact of our pending adoption of this standard on our consolidated financial statements.

In November 2016, the FASB issued ASU 2016-18, Statement of Cash Flows (Topic 230): Restricted Cash, which requires restricted cash to
be presented with cash and cash equivalents on the statement of cash flows and disclosure of how the statement of cash flows reconciles to the
balance sheet if restricted cash is shown separately from cash and cash equivalents on the balance sheet. The guidance is effective for interim and
annual periods beginning after December 15, 2017, and early adoption is permitted. The Company early adopted the ASU 2016-18 on December
15, 2017.

In  January  2017,  the  FASB  issued ASU  No.  2017-01,  Business  Combinations  (Topic  805):  Clarifying  the  Definition  of  a  Business,  which
clarifies the definition of a business to provide additional guidance with evaluating whether transactions should be accounted for as acquisitions
(or  disposals)  of  assets  or  businesses.  This ASU  is  effective  for  annual  periods  beginning  after  December  15,  2017,  including  interim  periods
within those periods. The Company early adopted the ASU 2016-18 on December 15, 2017 starting with its purchase of BaroFold assets.

In May 2014, the FASB issued ASU 2014-09, Revenue from Contracts with Customers (Topic 606) which amended the existing accounting
standards for revenue recognition. ASU 2014-09 establishes principles for recognizing revenue upon the transfer of promised goods or services to
customers,  in  an  amount  that  reflects  the  expected  consideration  received  in  exchange  for  those  goods  or  services.  In  July  2015,  the  FASB
deferred  the  effective  date  for  annual  reporting  periods  beginning  after  December  15,  2017  (including  interim  reporting  periods  within  those
periods). The amendments may be applied retrospectively to each prior period (full retrospective) or retrospectively with the cumulative effect
recognized as of the date of initial application (modified retrospective). The Company will adopt ASU 2014-09 in the first quarter of 2018 and
apply  the  modified  retrospective  approach.  The  Company’s  primary  source  of  revenues  is  from  instrument  sales  which  are  considered  distinct
performance obligations and are recognized upon shipment, the Company does not expect the impact on its consolidated financial statements to
be material.

Effective January 1, 2018, the Company adopted ASC Topic 606, Revenue from Contracts with Customers, using the modified retrospective
method. This guidance supersedes nearly all existing revenue recognition guidance under US GAAP. The core principle of the guidance is that an
entity should recognize revenue when it transfers promised goods or services to customers in an amount that reflects the consideration to which
the company expects to be entitled in exchange for those goods or services. The Company has drafted its accounting policy for the new standard
based on a detailed review of its business and contracts. Based on the new guidance, the Company continues to recognize revenue at a particular
point in time for the majority of its contracts with customers, which is generally when products are either shipped or delivered. Therefore, the
adoption  of  ASC  606  did  not  have  a  material  impact  on  the  consolidated  financial  statements.  The  Company  anticipates  it  will  expand  its
consolidated financial statement disclosures in order to comply with the disclosure requirements of the ASU beginning in the first quarter of 2018.

Effective January 1, 2018, the Company adopted ASU 2016-01, Recognition and Measurement of Financial Assets and Financial Liabilities.
The standard amends various aspects of the recognition, measurement, presentation, and disclosure of financial instruments. The most significant
impact  to  our  consolidated  financial  statements  relates  to  the  recognition  and  measurement  of  equity  investments  at  fair  value  with  changes
recognized in Net income. The amendment also updates certain presentation and disclosure requirements. The adoption of ASU 2016-01 did not
have a material impact on the consolidated financial statements. The adoption of ASU 2016-01 is expected to increase volatility in net income as
changes  in  the  fair  value  of  available-for-sale  equity  investments  and  changes  in  observable  prices  of  equity  investments  without  readily
determinable fair values will be recorded in net income.

- 45 -

 
 
 
 
 
 
 
 
 
 
In  July  2017,  the  FASB  issued ASU  2017-11,  Earnings  Per  Share  (Topic  260),  Distinguishing  Liabilities  from  Equity  (Topic  480)  and
Derivatives  and  Hedging  (Topic  815):  I.  Accounting  for  Certain  Financial  Instruments  with  Down  Round  Features;  II.  Replacement  of  the
Indefinite Deferral for Mandatorily Redeemable Financial Instruments of Certain Nonpublic Entities and Certain Mandatorily Redeemable Non-
controlling Interests with a Scope Exception. Part I of this update addresses the complexity of accounting for certain financial instruments with
down round features. Down round features are features of certain equity-linked instruments (or embedded features) that result in the strike price
being reduced on the basis of the pricing of future equity transactions. Current accounting guidance creates cost and complexity for entities that
issue financial instruments (such as warrants and convertible instruments) with down round features that require fair value measurement of the
entire  instrument  or  conversion  option.  Part  II  of  this  update  addresses  the  difficulty  of  navigating  Topic  480,  Distinguishing  Liabilities  from
Equity, because of the existence of extensive pending content in the FASB Accounting Standards Codification. This pending content is the result
of the indefinite deferral of accounting requirements about mandatorily redeemable financial instruments of certain nonpublic entities and certain
mandatorily  redeemable  non-controlling  interests.  The  amendments  in  Part  II  of  this  update  do  not  have  an  accounting  effect.  This ASU  is
effective for fiscal years, and interim periods within those years, beginning after December 15, 2018 with early adoption permitted. The Company
early adopted the ASU 2017-11 in the third quarter of 2017.

Adoption of ASU 2017-11

The Company changed its method of accounting for the Debentures, Debenture Warrants and Series D Warrants through the early adoption
of ASU 2017-11 during the year ended December 31, 2017 on a modified retrospective basis. Accordingly, the Company reclassified the warrant
derivative  and  conversion  option  derivative  liabilities  to  additional  paid  in  capital  on  its  January  1,  2017  consolidated  balance  sheets  totaling
approximately  $2.6  million,  reduced  debt  discount  by  approximately  $0.9  million  and  recorded  the  cumulative  effect  of  the  adoption  to  the
beginning  balance  of  accumulated  deficit  of  approximately  $2.4  million.  This  resulted  to  an  increase  in  stock  warrants  by  $2.6  million  and
additional paid-in capital by $1.5 million. In addition, because of the modified retrospective adoption, the Company credited the change in fair
value  of  warrant  derivative  and  conversion  option  derivative  liabilities  on  its  consolidated  statements  of  operations  by  $311,182  and  reduced
amortization of debt discount by $812,904 for the year ended December 31, 2017. The following table provides a reconciliation of the warrant
derivative  liability,  convertible  debt,  conversion  option  derivative  liability,  stock  warrant,  additional  paid-in  capital  and  accumulated  deficit  on
the consolidated balance sheet as of December 31, 2016:

Convertible
debt,
current
portion    

Convertible
debt, long
term
portion    

Warrant
Derivative
Liability    

Conversion
Option
Liability    

Warrants
to acquire
common
stock

Additional
Paid-in
Capital

Accumulated
deficit

Balance, January 1, 2017 (Prior to
adoption of ASU 2017-11)
Reclassified derivative liabilities and
cumulative effect of adoption
Balance, January 1, 2017 (After adoption
of ASU 2017-11)

  $ 4,005,702    $

529,742    $ 1,685,108    $

951,059    $6,325,102    $27,544,265    $ (42,264,190)

769,316     

154,152    $(1,685,108)    

(951,059)     2,636,236      1,446,011     

(2,369,548)

  $ 4,775,018    $

683,894    $

-    $

-    $8,961,338    $28,990,276    $ (44,633,738)

ITEM 7A. QUANTITATIVE AND QUALITATIVE DISCLOSURES ABOUT MARKET RISK.

Not Applicable

- 46 -

 
 
 
 
 
 
 
   
   
 
   
 
 
 
ITEM 8. FINANCIAL STATEMENTS AND SUPPLEMENTARY DATA

Report of Independent Registered Public Accounting Firm

To the Shareholders and Board of Directors of
Pressure Biosciences, Inc.

Opinion on the Financial Statements

We have audited the accompanying consolidated balance sheets of Pressure Biosciences, Inc. and its subsidiary (collectively, the “Company”) as
of December 31, 2017 and 2016, and the related consolidated statements of operations, comprehensive loss, changes in stockholders’ deficit, and
cash  flows  for  the  years  then  ended,  and  the  related  notes  (collectively  referred  to  as  the  “financial  statements”).  In  our  opinion,  the  financial
statements present fairly, in all material respects, the financial position of the Company as of December 31, 2017 and 2016, and the results of
their operations and their cash flows for the years then ended, in conformity with accounting principles generally accepted in the United States of
America.

Going Concern Matter

The accompanying financial statements have been prepared assuming that the Company will continue as a going concern. As discussed in Note 2
to the financial statements, the Company has a working capital deficit, has incurred recurring net losses and negative cash flows from operations.
These conditions raise substantial doubt about its ability to continue as a going concern. Management's plans in regard to these matters are also
described in Note 2. The financial statements do not include any adjustments that might result from the outcome of this uncertainty.

Basis for Opinion

These financial statements are the responsibility of the Company’s management. Our responsibility is to express an opinion on the Company’s
financial  statements  based  on  our  audits.  We  are  a  public  accounting  firm  registered  with  the  Public  Company Accounting  Oversight  Board
(United States) ("PCAOB") and are required to be independent with respect to the Company in accordance with the U.S. federal securities laws
and the applicable rules and regulations of the Securities and Exchange Commission and the PCAOB.

We conducted our audits in accordance with the standards of the PCAOB. Those standards require that we plan and perform the audit to obtain
reasonable assurance about whether the financial statements are free of material misstatement, whether due to error or fraud. The Company is not
required to have, nor were we engaged to perform, an audit of its internal control over financial reporting. As part of our audits we are required to
obtain an understanding of internal control over financial reporting but not for the purpose of expressing an opinion on the effectiveness of the
Company's internal control over financial reporting. Accordingly, we express no such opinion.

Our audits included performing procedures to assess the risks of material misstatement of the financial statements, whether due to error or fraud,
and performing procedures that respond to those risks. Such procedures included examining, on a test basis, evidence regarding the amounts and
disclosures  in  the  financial  statements.  Our  audits  also  included  evaluating  the  accounting  principles  used  and  significant  estimates  made  by
management, as well as evaluating the overall presentation of the financial statements. We believe that our audits provide a reasonable basis for
our opinion.

/s/ MaloneBailey, LLP
www.malonebailey.com
We have served as the Company's auditor since 2015.
Houston, Texas
April 2, 2018

- 47 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY
CONSOLIDATED BALANCE SHEETS
DECEMBER 31, 2017 AND 2016

December 31, 2017

December 31, 2016

$

$

$

ASSETS

CURRENT ASSETS
Cash and cash equivalents
Accounts receivable, net of $0 reserve at December 31, 2017 and $28,169 at
December 31, 2016
Inventories, net of $179,600 reserve at December 31, 2017 and $20,000 December
31, 2016
Prepaid income taxes
Prepaid expenses and other current assets

Total current assets

Investment in available-for-sale equity securities
Property and equipment, net
Intangible assets, net
TOTAL ASSETS

LIABILITIES AND STOCKHOLDERS’ DEFICIT

CURRENT LIABILITIES
Accounts payable
Accrued employee compensation
Accrued professional fees and other
Other current liabilities
Deferred revenue
Revolving note payable, net of unamortized debt discounts of $0 and $637,030,
respectively
Related party convertible debt, net of unamortized debt discounts of $31,372 and $0,
respectively
Convertible debt, net of unamortized discounts of $401,856 and $2,235,839,
respectively
Other debt, net of unamortized discounts of $48,194 and $380, respectively
Warrant derivative liabilities
Conversion option derivative liabilities

Total current liabilities
LONG TERM LIABILITIES
Related party convertible debt, net of unamortized debt discounts of $0 and
$165,611, respectively
Convertible debt, net of unamortized discounts of $0 and $740,628, respectively
Deferred revenue
TOTAL LIABILITIES
COMMITMENTS AND CONTINGENCIES (Note 7)
STOCKHOLDERS’ DEFICIT
Series D Convertible Preferred Stock, $.01 par value; 850 shares authorized; 300
shares issued and outstanding on December 31, 2017 and 2016, respectively
(Liquidation value of $300,000)
Series G Convertible Preferred Stock, $.01 par value; 240,000 shares authorized;
80,570 and 86,570 shares issued and outstanding on December 31, 2017 and 2016,
respectively
Series H Convertible Preferred Stock, $.01 par value; 10,000 shares authorized;
10,000 shares issued and outstanding on December 31, 2017 and 2016, respectively  
Series H2 Convertible Preferred Stock, $.01 par value; 21 shares authorized; 21
shares issued and outstanding on December 31, 2017 and 2016, respectively
Series J Convertible Preferred Stock, $.01 par value; 6,250 shares authorized; 3,458
and 3,521 shares issued and outstanding on December 31, 2017 and 2016,
respectively
Series K Convertible Preferred Stock, $.01 par value; 15,000 shares authorized; 6,880
and 6,816 shares issued and outstanding on December 31, 2017 and 2016,
respectively
Common stock, $.01 par value; 100,000,000 shares authorized; 1,342,858 and
1,033,328 shares issued and outstanding on December 31, 2017 and 2016,
respectively
Warrants to acquire common stock
Additional paid-in capital
Accumulated other comprehensive loss
Accumulated deficit

Total stockholders’ deficit

81,033    $

206,848   

857,662   
7,482   
214,676   
1,367,701   
19,825   
22,662   
750,000   
2,160,188    $

589,263    $
368,700   
800,620   
1,536,507   
263,106   

3,500,000   

259,762   

8,028,014    
1,379,863    
-   
-   
16,725,835   

-   
-   
57,149   
16,782,984   

3   

806   

100   

-   

35   

68   

13,429   
9,878,513   
30,833,549   
-   
(55,349,299)  
(14,622,796)  

TOTAL LIABILITIES AND STOCKHOLDERS’ DEFICIT

$

2,160,188    $

The accompanying notes are an integral part of these consolidated financial statements.

138,363 

281,320 

905,284 
7,405 
258,103 
1,590,475 
25,865 
9,413 
- 
1,625,753 

407,249 
249,596 
610,589 
346,295 
159,654 

612,970 

- 

4,005,702 
238,157 
1,685,108 
951,059 
9,266,379 

125,523 
529,742 
87,527 
10,009,171 

3 

866 

100 

- 

35 

68 

10,333  
6,325,102 
27,544,265 
- 
(42,264,190)
(8,383,418)
1,625,753 

 
 
 
 
 
   
 
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
- 48 -

 
PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY
CONSOLIDATED STATEMENTS OF OPERATIONS
FOR THE YEARS ENDED DECEMBER 31, 2017 AND 2016

Revenue:
Products, services, other
Grant revenue

Total revenue

Costs and expenses:
Cost of products and services
Research and development
Selling and marketing
General and administrative

Total operating costs and expenses

Operating loss

Other (expense) income:
Interest expense
Other expense
Impairment loss on investment
Gain on extinguishment of debt
Incentive warrants for warrant exercises
Change in fair value of derivative liabilities

Total other (expense) income

Net loss

Net loss per share - basic and diluted

For the Year Ended
December 31,

2017

2016

$

2,065,891    $
174,607   
2,240,498   

1,273,354   
988,597   
1,209,334   
3,416,261   
6,887,546   

1,794,749 
181,738 
1,976,487 

834,012 
1,183,011 
872,365 
2,822,752 
5,712,140 

(4,647,048)  

(3,735,653)

(6,055,420)  
(5,674)  
(6,069)  
185,452   
(186,802)  
-   
(6,068,513)  

(4,501,186)
(1,112)
(373,682)
- 
- 
5,904,649 
1,028,669 

$

$

(10,715,561)   $

(2,706,984)

(9.62)   $

(2.97)

Weighted average common stock shares outstanding used in the basic and diluted net loss per share
calculation

1,114,225   

911,312 

The accompanying notes are an integral part of these consolidated financial statements.

- 49 -

 
 
 
 
 
 
 
 
 
 
 
   
 
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
 
 
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
 
 
    
 
  
 
 
 
 
    
 
  
 
 
 
 
 
 
PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY
CONSOLIDATED STATEMENTS OF COMPREHENSIVE LOSS
FOR THE YEARS ENDED DECEMBER 31, 2017 AND 2016

Comprehensive Loss

Net loss

Other comprehensive loss
Reclassification of unrealized loss to realized loss on marketable securities

Comprehensive loss

- 50 -

For the Year Ended
December 31,

2017

2016

$

(10,715,561)   $

(2,706,984)

-   

105,025 

$

(10,715,561)   $

(2,601,959)

 
 
 
 
 
 
 
 
 
 
 
   
 
 
 
    
 
  
 
 
 
    
 
  
 
 
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
 
    
 
  
 
 
PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY
CONSOLIDATED STATEMENTS OF CHANGES IN STOCKHOLDERS’ DEFICIT
FOR THE YEARS ENDED DECEMBER 31, 2017 AND 2016

Series D Preferred
Stock

Series G Preferred
Stock

Series H Preferred
Stock

Series H(2)Preferred
Stock

  Shares     Amount
300    $
-     

    Shares     Amount     Shares     Amount     Shares     Amount
10,000    $
3     
-     
-     

86,570    $
-     

100     
-     

866     
-     

21    $
-     

BALANCE, December 31, 2015

Stock-based compensation
Issuance of common stock for
services
Warrant revaluation
Warrant exercise
Stock exchange with Everest
Investments
Issuance of warrants for services
Conversion of debt and interest for
common stock
Issuance of common stock for
dividends paid-in-kind
Conversion of Series J convertible
preferred stock
Conversion of Series K convertible
preferred stock
Common Stock offering
Offering costs for issuance of
common stock
Stock issued with debt
Warrants issued with debt
Unrealized loss on investments, net
of tax
Net loss

BALANCE, December 31, 2016
Early adoption of ASU 2017-11
Stock-based compensation
Issuance of common stock for services    
Warrant revaluation
Warrant exercise, net of costs
Stock exchange with Everest
Investments
Issuance of warrants for services
Conversion of debt and interest for
common stock
Issuance of common stock for interest
paid-in-kind
Conversion of Series G convertible
preferred stock
Conversion of Series J convertible
preferred stock
Incentive warrants
Offering costs for issuance of common
stock
Stock issued for debt extension
Stock issued with debt
Common stock for asset purchase
Warrants issued with debt
Net loss
BALANCE, December 31, 2017

-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     

-     
-     
300    $
-     
-     
-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     
-     
-     
-     
300    $

-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     

-     
-     
3     
-     
-     
-     
-     
-     

-     
-     

-     

-     

-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     

-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     

-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     

-     
-     
86,570    $
-     
-     
-     
-     
-     

-     
-     
866     
-     
-     
-     
-     
-     

-     
-     
10,000    $
-     
-     
-     
-     
-     

-     
-     

-     

-     

-     
-     

-     

-     

-     

(6,000)    

(60)    

-     
-     

-     
-     
-     
-     
-     
-     
3     

-     
-     

-     
-     
-     
-     
-     
-     
80,570    $

- 51 -

-     
-     

-     
-     
-     
-     
-     
-     
806     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     
-     
-     
-     
10,000    $

-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     

-     
-     
100     
-     
-     
-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     
-     
-     
-     
100     

-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     

-     
-     
21    $
-     
-     
-     
-     
-     

-     
-     

-     

-     

-     

-     
-     

-     
-     
-     
-     
-     
-     
21    $

- 
- 

- 
- 
- 

- 
- 

- 

- 

- 

- 
- 

- 
- 
- 

- 
- 
- 
- 
- 
- 
- 
- 

- 
- 

- 

- 

- 

- 
- 

- 
- 
- 
- 
- 
- 
   - 

 
 
 
 
 
   
   
   
 
 
 
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
   
 
Series J
Preferred Stock   
 Shares    Amount   Shares   Amount   Shares

Series K

Preferred Stock    Common Stock   

Additional
Paid-In
Stock
  Amount   Warrants    Capital

Accumulated
other

comprehensive  Accumulated  

Total
Stockholders’ 

loss

   Deficit

   Deficit

BALANCE,
December 31, 2015   3,546   $

36   11,416  $

114    766,830  $ 7,668  $5,416,681  $26,259,115  $

(105,025) $(39,557,206) $ (7,977,648)

-    

-    

-    

-    

-    

-    

-    

-    

(25)   

-    
-    

Stock-based
compensation
Issuance of
common stock
for services
Warrant exercise  
Issuance of
warrants for
services
Conversion of
debt and interest
for common
stock
Issuance of
common stock
for dividends
paid-in-kind
Conversion of
Series J
convertible
preferred stock   
Conversion of
Series K
convertible
preferred stock   
Common stock
offering
Offering costs
for issuance of
common stock   
Stock issued
with debt
Warrants issued
with debt
Beneficial
conversion
feature
Unrealized loss
on investments,
net of tax
Net loss
BALANCE,
December 31, 2016   3,521   $
Early adoption of
ASU 2017-11
Stock-based
compensation
Issuance of
common stock for
services
Warrant exercise,
net of costs
Issuance of
warrants for
services
Issuance of
common stock for
interest paid-in-
kind
Conversion of
Series G
convertible
preferred stock
Conversion of
Series J convertible
preferred stock

-    
-    

-    

-    

-    

-    

-    

-    

-    

-    

-    

(63)   

-   

-   

-   

-   

-   

-   

379,964   

-   
-   

-   
-   

-   
-   

25,167   
766   

252   
8   

-   
(11,100)  

332,444   
11,092   

-   

-   

-   

-   

-   

84,735   

-   

-   

-   

-   

14,028   

140   

-   

117,697   

-   

-   

-   

8,285   

83   

-   

63,772   

(1)  

-   

-   

833   

8   

-   

(7)  

-    (4,600)  

(46)   153,333    1,533   

-   

(1,487)  

-   

-   

-   

50,834   

508   

315,301   

294,191   

-   

-   

-   

-   

-   

-   

-   

-   

-   

-   

(79,035)  

-   

13,252   

133   

-   

145,798   

-   

-   

-   

519,485   

-   

-   

-   

-   

-   

-   

-   

20,721   

-   

-   
-   

-   

-   

-   

-   

-   

-   

-   

-   

-   

-   

-   

379,964 

-   
-   

332,696 
- 

-   

84,735 

-   

117,837 

-   

63,855 

-   

-   

-   

-   

-   

-   

- 

- 

610,000 

(79,035)

145,931 

519,485 

-   

20,721 

-   
-   

-   
-   

-   
-   

-   
-   

-   
-   

-   
-   

-   
-   

105,025   
-   

-   
(2,706,984)  

105,025 
(2,706,984)

35    6,816  $

68   1,033,328  $10,333  $6,325,102  $27,544,264  $

-  $(42,264,190) $ (8,383,418)

-    2,636,236    1,446,011   

-   

(2,369,548)  

1,712,699 

-   

-   

-   

-   

406,427   

-   

-   

-   

-   

-   

-   

-   

64   

-   

-   

-   

-   

-   

-   

-   

5,667   

57   

19,889   

199   

-   

-   

46,935   

140,015   

-   

-   

15,558   

-   

-   

-   

-   

-   

-   

-   

-   

-   

406,427 

-   

-   

-   

46,992 

140,214 

15,558 

-   

264,602 

-   

-   

- 

- 

-   

-   

-   

61,307   

612   

-   

263,990   

-   

-   

-   

2,000   

20   

-   

-   

-   

2,100   

21   

-   

-   

40   

(21)  

 
 
 
 
  
  
 
  
 
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
-    

-    

-    

Incentive warrants   
Stock issued for
debt extension
Stock issued with
debt
Common stock for
asset purchase
Warrants issued
with debt
Net loss
BALANCE,
December 31, 2017   3,458   $

-    
-    

-    

-   

-   

-   

-   

-   
-   

-   

-   

-   

-   

-   
-   

-   

-   

186,802   

-   

-   

-   

-   

4,167   

42   

64,400   

645   

-    150,000    1,500   

-   

-   

-   

19,458   

367,929   

598,500   

-   

-   

-   

-   

-   

-   

-   

-   

186,802 

19,500 

368,574 

600,000 

-   
-   

-   
-   

-   
-   

714,815   
-   

-   
-   

-   
714,815 
-   
-    (10,715,561)   (10,715,561)

35    6,880  $

68   1,342,858  $13,429  $9,878,513  $30,833,549  $

-  $(55,349,299) $ (14,622,796)

The accompanying notes are an integral part of these consolidated financial statements

- 52 -

  
  
  
  
  
 
 
PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY
CONSOLIDATED STATEMENTS OF CASH FLOWS
FOR THE YEARS ENDED DECEMBER 31, 2017 AND 2016

CASH FLOWS FROM OPERATING ACTIVITIES:

Net loss

Adjustments to reconcile net loss to net cash used in operating activities:
Common stock issued for debt extension
Depreciation and amortization
Provision for bad debts
Inventory reserve
Accretion of interest and amortization of debt discount
Incentive warrants for warrant exercises
Penalty interest added to debt principal
Gain on settlement of debt
Stock-based compensation expense
Warrants issued for service
Shares issued for service
Amortization of third party fees paid in common stock and warrants
Impairment loss on investment
Change in fair value of derivative liabilities
Changes in operating assets and liabilities:

Accounts receivable
Inventories
Prepaid expenses and other assets
Accounts payable
Accrued employee compensation
Accrued interest
Deferred revenue and other accrued expenses

Net cash used in operating activities

CASH FLOWS FROM INVESTING ACTIVITIES:

Purchases of intangible assets
Purchases of property plant and equipment
Net cash used in investing activities

CASH FLOWS FROM FINANCING ACTIVITIES:

Net proceeds from related party debt
Payment of related party debt
Net proceeds from revolving note payable
Net proceeds from convertible debt
Payments on convertible debt
Net proceeds from non-convertible debt
Payments on non-convertible debt
Net proceeds from warrant exercises
Payments on prepayment penalty
Net proceeds from the issuance of common stock
Net cash provided by financing activities

NET (DECREASE) INCREASE IN CASH
CASH AT BEGINNING OF YEAR
CASH AT END OF YEAR

SUPPLEMENTAL INFORMATION

Interest paid in cash

NON CASH TRANSACTIONS:

Convertible debt exchanged for common stock
Cashless exercise of warrants
Discount due to beneficial conversion feature
Discount due to warrants issued with debt
Common stock issued with debt
Common stock issued to purchase intangible assets
Common stock issued to settle non-convertible debt
Common stock issued in lieu of cash for interest
Common stock issued for prepaid services
Conversion of preferred stock and accrued dividends into common stock
Discount from derivative liability
Discount from one-time interest
Reclassification of derivative liabilities to equity and cumulative effect of adoption of ASU
2017-11

For the Year Ended
December 31,

2017

2016

$

(10,715,561)   $

(2,706,984)

19,500   
8,576   
-   
159,600   
4,128,637   
186,802   
-   
(185,452)  
406,427   
15,558   
31,000   
-   
6,069   
-   

74,472   
(111,978)  
59,312   
182,014   
119,104   
898,212   
813,159   
(3,904,549)  

(150,000)  
(21,825)  
(171,825)  

-   

2,070,000   
1,755,850   
(925,541)  
2,905,752   
(1,894,231)  
140,214   
(33,000)  
-   
4,019,044   

(57,330)  
138,363   
81,033    $

- 
17,939 
28,169 
- 
4,003,485 
- 
41,200 
(5,044)
379,964 
84,735 
- 
332,696 
373,682 
(5,904,649)

(196,233)
133,087 
(44,201)
(534,140)
73,587 
346,295 
(229,439)
(3,805,851)

- 
(7,203)
(7,203)

116,667 
(20,000)
1,133,500 
2,105,420 
(107,000)
1,022,784 
(947,702)
- 
- 
530,965 
3,834,634 

21,580 
116,783 
138,363 

533,532    $

260,979 

-   
-   
-   
714,815   
368,574   
600,000   
-   
264,602   
15,992   
61   
-   
225,000   

117,837 
11,100 
20,721 
519,485 
104,731 
- 
41,200 
- 
- 
63,902 
1,304,049 
170,000 

$

$

 
 
 
 
 
 
 
 
 
 
 
   
 
 
 
    
 
  
 
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
    
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
    
 
  
 
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Reversal of accumulated other comprehensive income to impairment loss on investment

1,712,699   
-   

- 
105,025 

The accompanying notes are an integral part of these consolidated financial statements.

- 53 -

 
 
 
 
 
 
 
 
 
PRESSURE BIOSCIENCES, INC. AND SUBSIDIARY
NOTES TO CONSOLIDATED FINANCIAL STATEMENTS

(1) Business Overview

Pressure  Biosciences,  Inc.  (“we”,  “our”,  “the  Company”)  is  focused  on  solving  the  challenging  problems  inherent  in  biological  sample
preparation, a crucial laboratory step performed by scientists worldwide working in biological life sciences research. Sample preparation is a term
that refers to a wide range of activities that precede most forms of scientific analysis. Sample preparation is often complex, time-consuming, and
in our belief, one of the most error-prone steps of scientific research. It is a widely-used laboratory undertaking, the requirements of which drive
what  we  believe  is  a  large  and  growing  worldwide  market.  We  have  developed  and  patented  a  novel,  enabling  technology  platform  that  can
control the sample preparation process. It is based on harnessing the unique properties of high hydrostatic pressure. This process, called pressure
cycling technology, or PCT, uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels (35,000 psi or greater) to safely,
conveniently and reproducibly control the actions of molecules in biological samples, such as cells and tissues from human, animal, plant, and
microbial sources.

Our pressure cycling technology uses internally developed instrumentation that is capable of cycling pressure between ambient and ultra-high
levels - at controlled temperatures and specific time intervals - to rapidly and repeatedly control the interactions of bio-molecules, such as DNA,
RNA,  proteins,  lipids,  and  small  molecules.  Our  laboratory  instrument,  the  Barocycler®®,  and  our  internally  developed  consumables  product
line,  including  PULSE®  (Pressure  Used  to  Lyse  Samples  for  Extraction)  Tubes,  other  processing  tubes,  and  application  specific  kits  (which
include consumable products and reagents) together make up our PCT Sample Preparation System, or PCT SPS.

In 2015, together with an investment bank, we formed a subsidiary called Pressure BioSciences Europe (“PBI Europe”) in Poland. We have
49% ownership interest with the investment bank retaining 51%. As of now, PBI Europe does not have any operating activities and we cannot
reasonably predict when operations will commence. Therefore, we do not have control of the subsidiary and did not consolidate in our financial
statements. PBI Europe did not have any operations in 2017 or in 2016.

Reverse Stock Split

On  June  5,  2017,  the  Company  effected  a  1-for-30  reverse  stock  split  of  its  issued  and  outstanding  shares  of  common  stock All  common
shares, stock options, and per share information presented in  the  financial  statements  have  been  adjusted  to  reflect  the  reverse  stock  split  on  a
retroactive basis for all periods presented. The ratio by which shares of preferred stock are convertible into shares of common stock were adjusted
to reflect the effects of the reverse stock split.

(2) Going Concern

The  accompanying  financial  statements  have  been  prepared  assuming  that  the  Company  will  continue  as  a  going  concern,  which
contemplates the realization of assets and the liquidation of liabilities in the normal course of business. However, we have experienced negative
cash flows from operations with respect to our pressure cycling technology business since our inception. As of December 31, 2017, we do not
have adequate working capital resources to satisfy our current liabilities and as a result, there is substantial doubt regarding our ability to continue
as  a  going  concern.  We  have  been  successful  in  raising  cash  through  debt  and  equity  offerings  in  the  past  and  as  described  in  Notes  8  and  9,
completed debt financing subsequent to December 31, 2017. We have financing efforts in place to continue to raise cash through debt and equity
offerings.

Management has developed a plan to continue operations. This plan includes obtaining equity or debt financing. During the year ended December
31,  2017  we  received  approximately  $6.7  million   net  proceeds,  in  additional  convertible  and  non-convertible  debt.  Although  we  have
successfully completed financings and reduced expenses in the past, we cannot assure you that our plans to address these matters in the future will
be successful.

Management’s plans to alleviate these conditions that raise substantial doubt regarding the Company’s ability to continue as a going concern
include  pursuing  one  or  more  of  the  following  options  to  raise  additional  funding,  none  of  which  can  be  guaranteed  or  are  entirely  within  the
Company’s control:

● Raise funding through the possible additional sales of the Company’s common stock, including public or private equity financings.

● Raise additional loan funding.

● Continue to seek a partner to advance PCT technology.

● Earn payments pursuant to potential collaboration and license agreements for BaroFold patents.

There can be no assurance, however, that the Company will receive cash proceeds from any of these potential resources or, to the extent cash
proceeds are received, those proceeds would be sufficient to support the Company’s operations for at least the next twelve months from the date
of filing this Annual Report on Form 10-K.

In  August  2014,  the  Financial  Accounting  Standards  Board  (“FASB”)  issued  Accounting  Standards  Update  (“ASU”)  No.  2014-15,
Presentation of Financial Statements-Going Concern (Subtopic 205-40): Disclosure of Uncertainties about an Entity’s Ability to Continue as a
Going Concern. (“ASU 2015-14”). Under the new standard, management must evaluate whether there are conditions or events, considered in the
aggregate, that raise substantial doubt about the Company’s ability to continue as a going concern within one year after the date that the financial
statements are issued. This evaluation initially does not take into consideration the potential mitigating effect of management’s plans that have not
been fully implemented as of the date the financial statements are issued. When substantial doubt exists under this methodology, management
evaluates whether the mitigating effect of its plans sufficiently alleviates substantial doubt about the Company’s ability to continue as a going
concern.  The  mitigating  effect  of  management’s  plans,  however,  is  only  considered  if  both  (1)  it  is  probable  that  the  plans  will  be  effectively
implemented within one year after the date that the financial statements are issued, and (2) it is probable that the plans, when implemented, will
mitigate the relevant conditions or events that raise substantial doubt about the entity’s ability to continue as a going concern within one year after

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
the date that the financial statements are issued. This standard was adopted by the Company at January 1, 2017.

Generally, under the new accounting standard, management’s plans must be approved before the date the financial statements are issued to be
considered  probable  of  being  effectively  implemented.  Under  the  new  accounting  standard,  the  future  receipt  of  potential  funding  from  the
Company’s  collaborators  and  other  resources  is  not  considered  probable  at  this  time  because  none  of  the  Company’s  current  plans  have  been
finalized at the time of filing this Annual Report on Form 10-K. Accordingly, substantial doubt is deemed to exist about the Company’s ability to
continue as a going concern within one year after the date these financial statements are issued.

The Company believes that its approximate $80,033 in cash and cash equivalents at December 31, 2017 would allow it to fund its planned
operations  into  the  first  quarter  of  2018.  This  estimate  assumes  no  additional  funding  from  new  partnership  agreements,  no  additional  equity
financings, no debt financings, and no accelerated repayment of its term loans. Accordingly, the timing and nature of activities contemplated for
the remainder of 2017 and thereafter will be conducted subject to the availability of sufficient financial resources.

If the Company is unable to raise capital when needed or on attractive terms, or if it is unable to procure partnership arrangements to advance
its  programs,  the  Company  would  be  forced  to  delay,  reduce  or  eliminate  its  research  and  development  programs  and  any  future
commercialization efforts.

The  accompanying  financial  statements  have  been  prepared  on  a  going  concern  basis,  which  contemplates  the  realization  of  assets  and
satisfaction of liabilities in the ordinary course of business. The financial statements do not include any adjustments relating to the recoverability
and classification of recorded asset amounts or the amounts and classification of liabilities that might result from the outcome of the uncertainties
described above.

(3) Summary of Significant Accounting Policies

i. Principles of Consolidation

The consolidated financial statements include the accounts of Pressure BioSciences, Inc., and its wholly-owned subsidiary PBI BioSeq, Inc.

All intercompany accounts and transactions have been eliminated in consolidation.

- 54 -

 
 
 
 
 
 
 
 
ii. Use of Estimates

To  prepare  our  consolidated  financial  statements  in  conformity  with  accounting  principles  generally  accepted  in  the  United  States  of
America, we are required to make significant estimates and assumptions that affect the reported amounts of assets and liabilities and disclosure of
contingent assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the reporting
period. In addition, significant estimates were made in projecting future cash flows to quantify impairment of assets, deferred tax assets, the costs
associated with fulfilling our warranty obligations for the instruments that we sell, and the estimates employed in our calculation of fair value of
stock  options  awarded,  beneficial  conversion  features  and  derivative  liabilities.  We  base  our  estimates  on  historical  experience  and  on  various
other assumptions that we believe to be reasonable under the circumstances, the results of which form the basis for making judgments about the
carrying  values  of  assets  and  liabilities  that  are  not  readily  apparent  from  other  sources. Actual  results  could  differ  from  the  estimates  and
assumptions used.

iii. Revenue Recognition

Revenue is recognized when realized or when realizable and earned when all the following criteria have been met: persuasive evidence of an
arrangement  exists;  goods  were  shipped,  delivery  of  service  has  occurred  and  risk  of  loss  has  passed  to  the  customer;  the  seller’s  price  to  the
buyer is fixed or determinable; and collectability is reasonably assured.

Our current instruments, the Barocycler NEP3229 and NEP2320EXT, require a basic level of instrumentation expertise to set-up for initial
operation.  To  support  a  favorable  first  experience  for  our  customers,  upon  customer  request  and  for  an  additional  fee,  we  will  send  a  highly
trained  technical  representative  to  the  customer  site  to  install  Barocyclers  that  we  sell,  lease,  or  rent  through  our  domestic  sales  force.  The
installation process includes uncrating and setting up the instrument, followed by introductory user training. Product revenue related to current
Barocycler  instrumentation  is  recognized  upon  shipment  of  the  unit,  and  in  the  case  where  the  customer  requests  installation  and  training,  the
completion of the installation and introductory training process of the instrumentation at the customer location, for domestic installations. Product
revenue related to sales of PCT instrumentation to our foreign distributors is recognized upon shipment through a common carrier. We provide for
the expected costs of warranty upon the recognition of revenue for the sales of our instrumentation. Our sales arrangements do not provide our
customers with a right of return. Product revenue related to the HUB440 and our consumable products such as PULSE Tubes, MicroTubes, and
application specific kits is recorded upon shipment through a common carrier. Shipping costs are included in sales and marketing expense. Any
shipping costs billed to customers are recognized as revenue.

The Company applies ASC 845, “Accounting for Non-Monetary Transactions”, to account for products and services sold through non-cash
transactions  based  on  the  fair  values  of  the  products  and  services  involved,  where  such  values  can  be  determined.  Non-cash  exchanges  would
require revenue to be recognized at recorded cost or carrying value of the assets or services sold if any of the following conditions apply:

a) The fair value of the asset or service involved is not determinable.

b) The transaction is an exchange of a product or property held for sale in the ordinary course of business for a product or property to be sold

in the same line of business to facilitate sales to customers other than the parties to the exchange.

c) The transaction lacks commercial substance.

The Company currently records revenue for its non-cash transactions at recorded cost or carrying value of the assets or services sold.

We  account  for  our  lease  agreements  under  the  operating  method.  We  record  revenue  over  the  life  of  the  lease  term  and  we  record
depreciation  expense  on  a  straight-line  basis  over  the  thirty-six  month  estimated  useful  life  of  the  Barocycler  instrument.  The  depreciation
expense  associated  with  assets  under  lease  agreement  is  included  in  the  “Cost  of  PCT  products  and  services”  line  item  in  our  consolidated
statements of operations. Many of our lease and rental agreements allow the lessee to purchase the instrument at any point during the term of the
agreement with partial or full credit for payments previously made. We pay all maintenance costs associated with the instrument during the term
of the leases.

Revenue from government grants is recorded when qualifying expenses are incurred under the grant in accordance with the terms of the grant

award.

Deferred revenue represents amounts received from grants and the Company’s service contracts for which the related revenues have not been
recognized  because  one  or  more  of  the  revenue  recognition  criteria  have  not  been  met.  The  current  portion  of  deferred  revenue  represents  the
amount to be recognized within one year from the balance sheet date based on the estimated performance period of the underlying deliverables.
Revenue from service contracts is recorded ratably over the length of the contract.

Our  transactions  sometimes  involve  multiple  elements  (i.e.,  products  and  services).  Revenue  under  multiple  element  arrangements  is
recognized  in  accordance  with  FASB ASC  605-25  Multiple-Element Arrangements (“ASC 605”).  When  vendor  specific  objective  evidence  or
third party evidence of selling price for deliverables in an arrangement cannot be determined, the Company develops a best estimate of the selling
price  to  separate  deliverables  and  allocates  arrangement  consideration  using  the  relative  selling  price  method.  If  an  arrangement  includes
undelivered elements that are not essential to the functionality of the delivered elements, we defer the fair value of the undelivered elements to
such  time  as  they  are  delivered.  Fair  value  is  determined  based  upon  the  price  charged  when  the  element  is  sold  separately.  If  there  is  not
sufficient evidence of the fair value of the undelivered elements the Company uses its best estimate of the value of those items and recognizes
revenues based on the relative values of the delivered and undelivered items. We provide certain customers with extended service contracts with
revenue recognized ratably over the life of the contract.

- 55 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
iv. Cash and Cash Equivalents

Our policy is to invest available cash in short-term, investment grade interest-bearing obligations, including money market funds, and bank
and corporate debt instruments. Securities purchased with initial maturities of three months or less are valued at cost plus accrued interest, which
approximates fair value, and are classified as cash equivalents. Restricted cash is included in cash equivalents.

v. Research and Development

Research and development costs, which are comprised of costs incurred in performing research and development activities including wages
and associated employee benefits, facilities, consumable products and overhead costs that are expensed as incurred. In support of our research and
development activities we utilize our Barocycler instruments that are capitalized as fixed assets and depreciated over their expected useful life.

vi. Inventories

Inventories are valued at the lower of cost (average cost) or net realizable value. The cost of Barocyclers consists of the cost charged by the
contract  manufacturer.  The  current  year  allowance  was  increased  by  a  $159,600  inventory  allowance  for  the  older  generation  of  Barocycler
instruments held in stock, the NEP3229. The cost of manufactured goods includes material, freight-in, direct labor, and applicable overhead. The
composition of inventory as of December 31, is as follows:

Raw materials
Finished goods
Inventory reserve
Total

vii. Property and Equipment

2017

2016

  $

  $

288,295    $
748,967   
(179,600) 
857,662    $

326,228 
599,056 
(20,000)
905,284 

Property and equipment are stated at cost, less accumulated depreciation. For financial reporting purposes, depreciation is recognized using
the straight-line method, allocating the cost of the assets over their estimated useful lives of three years for certain laboratory equipment, from
three to five years for management information systems and office equipment, and three years for all PCT finished units classified as fixed assets.

viii. Intangible Assets

We  have  classified  as  intangible  assets,  costs  associated  with  the  fair  value  of  acquired  intellectual  property.  Intangible  assets,  including
patents, are being amortized on a straight-line basis over nine years. We perform an annual review of our intangible assets for impairment. We
capitalize any costs to renew or extend the term of our intangible assets. When impairment is indicated, any excess of carrying value over fair
value is recorded as a loss. As of December 31, 2017 and 2016, the outstanding balance for intangible assets was $750,000 and $0, respectively.

- 56 -

 
 
 
 
 
 
 
 
 
 
   
 
 
 
 
 
 
 
 
 
 
 
 
ix. Long-Lived Assets

The  Company’s  long-lived  assets  are  reviewed  for  impairment  in  accordance  with  the  guidance  of  the  FASB ASC  360-10-05,  Property,
Plant,  and  Equipment,  whenever  events  or  changes  in  circumstances  indicate  that  the  carrying  amount  of  the  asset  may  not  be  recoverable.
Recoverability of an asset to be held and used is measured by a comparison of the carrying amount of an asset to the future undiscounted cash
flows  expected  to  be  generated  by  the  asset.  If  such  asset  is  considered  to  be  impaired,  the  impairment  to  be  recognized  is  measured  by  the
amount  by  which  the  carrying  amount  of  the  asset  exceeds  its  fair  value.  Through  December  31,  2017,  the  Company  had  not  experienced
impairment losses on its long-lived assets.

x. Concentrations

Credit Risk

Our  financial  instruments  that  potentially  subject  us  to  concentrations  of  credit  risk  consist  primarily  of  cash,  cash  equivalents  and  trade
receivables. We have cash investment policies which, among other things, limit investments to investment-grade securities. We perform ongoing
credit evaluations of our customers, and the risk with respect to trade receivables is further mitigated by the fact that many of our customers are
government institutions and university labs. Allowances are provided for estimated amounts of accounts receivable which may not be collected.
At December 31, 2017, we determined that no allowance against accounts receivable was necessary and wrote off the prior year allowance of
$28,169 as unrecoverable.

The following table illustrates the level of concentration of the below two groups within revenue as a percentage of total revenues during the

years ended December 31:

Top Five Customers
Federal Agencies

2017

2016

37% 
14% 

29%
3%

The following table illustrates the level of concentration of the below two groups within accounts receivable as a percentage of total accounts

receivable balance as of December 31:

Top Five Customers
Federal Agencies

Investment in Available-For-Sale Equity Securities

2017

2016

85% 
1% 

82%
1%

As of December 31, 2017, we held 100,250 shares of common stock of Everest, a Polish publicly traded company listed on the Warsaw Stock
Exchange. We exchanged 33,334 shares of our common stock for the 100,250 shares from Everest. We account for this investment in accordance
with ASC  320  “Investments  —  Debt  and  Equity  Securities” as  securities  available  for  sale.  On  December  31,  2017,  our  consolidated  balance
sheet reflected the fair value of our investment in Everest to be $19,825, based on the closing price of Everest shares of $0.1978 per share on that
day.  The  carrying  value  of  our  investment  in  Everest  common  stock  held  will  change  from  period  to  period  based  on  the  closing  price  of  the
common  stock  of  Everest  as  of  the  balance  sheet  date.  The  change  in  market  value  since  the  receipt  of  stock  amounting  to  $379,751  was
determined to be other than temporary and was recorded by us as an impairment loss starting in 2016. We recorded $6,069 as realized losses in
2017 for the changes in market value.

xi. Computation of Loss per Share

Basic  loss  per  share  is  computed  by  dividing  loss  available  to  common  shareholders  by  the  weighted  average  number  of  common  shares
outstanding. Diluted loss per share is computed by dividing loss available to common shareholders by the weighted average number of common
shares  outstanding  plus  additional  common  shares  that  would  have  been  outstanding  if  dilutive  potential  common  shares  had  been  issued.  For
purposes of this calculation, convertible preferred stock, common stock dividends, warrants to acquire preferred stock convertible into common
stock, and warrants and options to acquire common stock, are all considered common stock equivalents in periods in which they have a dilutive
effect and are excluded from this calculation in periods in which these are anti-dilutive. The following table illustrates our computation of loss per
share for the years ended December 31:

- 57 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Numerator:
Net loss

Denominator for basic and diluted loss per share:
Weighted average common shares outstanding

2017

2016

$

(10,715,561)   $

(2,706,984)

1,114,225   

911,312 

Loss per common share - basic and diluted

$

(9.62)   $

(2.97)

The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially
dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive for the
years ended December 31:

Stock options
Convertible debt
Common stock warrants
Convertible preferred stock:

Series D Convertible Preferred
Series G Convertible Preferred
Series H Convertible Preferred
Series H2 Convertible Preferred
Series J Convertible Preferred
Series K Convertible Preferred

xii. Accounting for Income Taxes

2017

2016

247,692   
947,203   
899,542   

25,000   
26,857   
33,334   
70,000   
115,267   
229,334   
2,594,229   

175,642 
891,132 
881,990 

25,000 
28,857 
33,334 
70,000 
117,367 
227,200 
2,450,522 

We  account  for  income  taxes  under  the  asset  and  liability  method,  which  requires  recognition  of  deferred  tax  assets,  subject  to  valuation
allowances, and liabilities for the expected future tax consequences of events that have been included in the consolidated financial statements or
tax returns. Deferred income taxes reflect the net tax effects of temporary differences between the carrying amounts of assets and liabilities for
financial  reporting  and  income  tax  purposes.  The  Company  considers  many  factors  when  assessing  the  likelihood  of  future  realization  of  our
deferred  tax  assets,  including  recent  cumulative  earnings  experience  by  taxing  jurisdiction,  expectations  of  future  taxable  income  or  loss,  the
carry-forward  periods  available  to  us  for  tax  reporting  purposes,  and  other  relevant  factors. A  valuation  allowance  is  established  if  it  is  more
likely than not that all or a portion of the net deferred tax assets will not be realized. If substantial changes in the Company’s ownership should
occur, as defined in Section 382 of the Internal Revenue Code, there could be significant limitations on the amount of net loss carry forwards that
could be used to offset future taxable income.

Tax positions must meet a “more likely than not”  recognition  threshold  at  the  effective  date  to  be  recognized. At  December  31,  2017  and
2016,  the  Company  did  not  have  any  uncertain  tax  positions.  No  interest  and  penalties  related  to  uncertain  tax  positions  were  accrued  at
December 31, 2017 and 2016.

xiii. Accounting for Stock-Based Compensation

We  maintain  equity  compensation  plans  under  which  incentive  stock  options  and  non-qualified  stock  options  are  granted  to  employees,
independent members of our Board of Directors and outside consultants. We recognize equity compensation expense over the requisite service
period using the Black-Scholes formula to estimate the fair value of the stock options on the date of grant. Employee awards are accounted for
under ASC 718 where the awards are valued at grant date. Awards given to nonemployees are accounted for under ASC 505 where the awards are
valued at earlier of commitment date or completion of services.

- 58 -

 
 
 
 
   
 
 
 
    
 
  
 
 
 
 
    
 
  
 
 
    
 
  
 
 
 
 
 
 
    
 
  
 
 
 
 
 
   
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Determining Fair Value of Stock Option Grants

Valuation and Amortization Method  - The fair value of each option award is estimated on the date of grant using the Black-Scholes pricing
model based on certain assumptions. The estimated fair value of employee stock options is amortized to expense using the straight-line method
over the vesting period, which generally is over three years.

Expected  Term  -  The  Company  uses  the  simplified  calculation  of  expected  life,  described  in  the  FASB ASC  718,  Compensation-Stock
Compensation, as the Company does not currently have sufficient historical exercise data on which to base an estimate of expected term. Using
this method, the expected term is determined using the average of the vesting period and the contractual life of the stock options granted.

Expected Volatility - Expected volatility is based on the Company’s historical stock volatility data over the expected term of the award.

Risk-Free Interest Rate   -  The  Company  bases  the  risk-free  interest  rate  used  in  the  Black-Scholes  valuation  method  on  the  implied  yield

currently available on U.S. Treasury zero-coupon issues with an equivalent remaining term.

Forfeitures - As required by FASB ASC 718,  Compensation-Stock Compensation, the Company records stock-based compensation expense
only for those awards that are expected to vest. The Company estimated a forfeiture rate of 5% for awards granted based on historical experience
and future expectations of options vesting. We used this historical rate as our assumption in calculating future stock-based compensation expense.

The following table summarizes the assumptions we utilized for grants of stock options to the three sub-groups of our stock option recipients

during the year ended December 31, 2017:

Assumptions

Expected life
Expected volatility
Risk-free interest rate
Forfeiture rate
Expected dividend yield

Non-Employee
Board Members

6.0 (yrs) 

92.85%-104.83%    
1.01%-1.53%    
5.00%    
0.0%    

CEO, other
Officers and
Employees

6.0 (yrs)
105.71%
1.63%
5.00%
0.0%

We recognized stock-based compensation expense of $406,427 and $379,964 for the years ended December 31, 2017 and 2016, respectively.
The  following  table  summarizes  the  effect  of  this  stock-based  compensation  expense  within  each  of  the  line  items  within  our  accompanying
consolidated statements of operations for the years ended December 31:

Research and development
Selling and marketing
General and administrative
Total stock-based compensation expense

2017

2016

92,055    $
54,404   
259,968   
406,427    $

65,500 
42,315 
272,149 
379,964 

  $

  $

During the years ended December 31, 2017 and 2016, the total fair value of stock options awarded was $487,964 and $0, respectively.

As of December 31, 2017, total unrecognized compensation cost related to the unvested stock-based awards was $392,590, which is expected

to be recognized over weighted average period of 1.66 years.

- 59 -

 
 
 
 
 
 
 
 
 
 
 
 
 
   
   
   
   
   
   
 
 
 
 
   
 
 
 
 
 
 
 
 
 
 
xiv. Advertising

Advertising costs are expensed as incurred. We incurred $5,899 in 2017 and $19,125 in 2016 for advertising.

xv. Fair Value of Financial Instruments

Due  to  their  short  maturities,  the  carrying  amounts  for  cash  and  cash  equivalents,  accounts  receivable,  accounts  payable,  and  accrued
expenses  approximate  their  fair  value.  Short-term  and  long-term  liabilities  are  primarily  related  to  liabilities  transferred  under  contractual
arrangements with carrying values that approximate fair value.

xvi. Fair Value Measurements

The  Company  follows  the  guidance  of  FASB ASC  Topic  820,  “ Fair  Value  Measurements  and  Disclosures ” (“ASC 820”)  as  it  related  to

financial assets and financial liabilities that are recognized or disclosed at fair value in the consolidated financial statements on a recurring basis.

The  Company  generally  defines  fair  value  as  the  price  that  would  be  received  to  sell  an  asset  or  paid  to  transfer  a  liability  in  an  orderly
transaction between market participants at the measurement date (exit price). The Company uses a three-tier fair value hierarchy, which classifies
the inputs used in measuring fair values. These tiers include: Level 1, defined as observable inputs such as quoted prices for identical instruments
in active markets; Level 2, defined as inputs other than quoted prices in active markets that are either directly or indirectly observable; and Level
3, defined as unobservable inputs in which little or no market data exists, therefore requiring an entity to develop its own assumptions.

Financial  assets  and  liabilities  are  classified  in  their  entirety  based  on  the  lowest  level  of  input  that  is  significant  to  the  fair  value
measurement.  The  Company  has  determined  that  its  financial  assets  are  currently  classified  within  Level  1  and  that  its  financial  liabilities  are
currently all classified within Level 3 in the fair value hierarchy.

 The Company changed its method of accounting for the Debentures and Warrants through the early adoption of ASU 2017-11 during the
year ended December 31, 2017 on a modified retrospective basis. Accordingly, the Company reclassified the warrant derivative and conversion
option derivative liabilities to additional paid in capital on its January 1, 2017 consolidated balance sheets totaling approximately $2.6 million,
reduced debt discount by approximately $0.9 million and recorded the cumulative effect of the adoption to the beginning balance of accumulated
deficit of approximately $2.5 million.

Adoption of ASU 2017-11

The Company changed its method of accounting for the Debentures, Debenture Warrants and Series D Warrants through the early adoption
of ASU 2017-11 during the year ended December 31, 2017 on a modified retrospective basis. Accordingly, the Company reclassified the warrant
derivative  and  conversion  option  derivative  liabilities  to  additional  paid  in  capital  on  its  January  1,  2017  consolidated  balance  sheets  totaling
approximately  $2.6  million,  reduced  debt  discount  by  approximately  $0.9  million  and  recorded  the  cumulative  effect  of  the  adoption  to  the
beginning  balance  of  accumulated  deficit  of  approximately  $2.4  million.  This  resulted  to  an  increase  in  stock  warrants  by  $2.6  million  and
additional  paid-in  capital  by  $1.4  million.  The  following  table  provides  a  reconciliation  of  the  warrant  derivative  liability,  convertible  debt,
conversion  option  derivative  liability,  stock  warrant,  additional  paid-in  capital  and  accumulated  deficit  on  the  consolidated  balance  sheet  as  of
December 31, 2016:

Convertible
debt,
current
portion    

Convertible
debt, long
term

portion    

Warrant
Derivative
Liability    

Conversion
Option
Liability    

Warrants
to acquire
common
stock

Additional
Paid-in
Capital

Accumulated
deficit

Balance, January 1, 2017 (Prior to
adoption of ASU 2017-11)
Reclassified derivative liabilities and
cumulative effect of adoption
Balance, January 1, 2017 (After adoption
of ASU 2017-11)

  $ 4,005,702    $

529,742    $ 1,685,108    $

951,059    $6,325,102    $27,544,265    $ (42,264,190)

769,316    

154,152   $(1,685,108)    

(951,059)   2,636,236      1,446,011     

(2,369,548)

  $ 4,775,018    $

683,894    $

-    $

-    $8,961,338    $28,990,276    $ (44,633,738)

The following tables set forth the Company’s financial assets and financial liabilities that were accounted for at fair value on a recurring basis
as of December 31, 2017 and December 31, 2016. The development of the unobservable inputs for Level 3 fair value measurements and fair value
calculations are the responsibility of the Company’s management.

  December 31, 2017    

Fair value measurements at December 31, 2017 using:
Significant 
unobservable inputs
(Level 3)

Significant other
observable inputs
(Level 2)

Quoted prices in
active markets
(Level 1)

Available-For-Sale Equity Securities
Total Financial Assets

  $

19,825     
19,825    $

19,825     
19,825    $

-     
-    $

  December 31, 2016    

Fair value measurements at December 31, 2016 using:
Significant 
unobservable inputs
(Level 3)

Significant other
observable inputs
(Level 2)

Quoted prices in
active markets
(Level 1)

Available-For-Sale Equity Securities
Total Financial Assets

  $

25,865     
25,865    $

25,865     
25,865    $

-     
-    $

Quoted prices in
active markets

Significant other
observable inputs

Significant
unobservable

- 
- 

- 
- 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
   
   
 
   
 
 
 
 
 
   
 
 
   
   
 
   
 
 
 
 
   
 
 
   
   
 
   
 
Series D Preferred Stock Purchase Warrants
Warrants Issued with Convertible Debt
Conversion Option Derivative Liabilities
Total Derivatives

  $

  December 31, 2016    
  $

23,313     
1,661,795     
951,059     
2,636,167    $

- 60 -

(Level 1)

(Level 2)

-     
-     
-     
-    $

inputs (Level 3)  
23,313 
1,661,795 
951,059 
2,636,167 

-    $
-     
-     
-    $

 
   
   
   
   
 
The  following  table  provides  a  summary  of  the  changes  in  fair  value,  including  net  transfers  in  and/or  out,  of  the  derivative  financial

instruments, measured at fair value on a recurring basis using significant unobservable inputs:

  January 1, 2016    

Issuance 
fair value    

Change in 
fair value     December 31, 2016  

Series D Preferred Stock Purchase
Warrants
Warrants Issued with Convertible Debt
Conversion Option Derivative Liabilities
Total Derivatives

  $

  $

173,526    $
3,122,450     
3,940,791     
7,236,767    $

-    $
(150,213)   $
1,094,432     
(2,555,087)    
(4,536,859)    
1,547,127     
2,641,559    $ (7,242,159)   $

23,313 
1,661,795 
951,059 
2,636,167 

The issuance fair values for 2016 include the “day 1” derivative losses on the conversion option derivative liabilities of $1,337,510, which
are included in “change in fair value of derivative liabilities” in the consolidated statements of operations. There were no derivative liabilities as
of December 31, 2017.

- 61 -

 
 
 
 
   
   
 
 
The fair value of the derivative liabilities was determined using a binomial pricing model. The assumptions for the binomial pricing model
are represented in the table below for the warrants issued in the Series D private placement reflected on a per share  common  stock  equivalent
basis.

Assumptions
Expected life (in months)
Expected volatility
Risk-free interest rate
Exercise price
Fair value per warrant

  November 10, 2011  
60.0 
104.5%   
0.875%   
  $
24.30 
  $
16.20 

  $
  $

Warrants revalued at 
December 31, 2016

5.0 
83.5%
0.62%
7.50 
0.60 

The assumptions for the binomial pricing model are represented in the table below for the warrants issued with the Convertible Debt in 2016

reflected on a per share common stock equivalent basis.

Assumptions
Expected life (in months)
Expected volatility
Risk-free interest rate
Exercise price
Fair value per warrant

At Issuance
Fair value

Warrants revalued at
December 31, 2016

60.0 

118.3-120.1%   
1.48-1.69%   
  $
12.00 
  $
5.70-$6.30 

  $
  $

43.0-51.0 
110.0-116.0%
1.93%
12.00 
3.60-4.20 

The  assumptions  for  the  binomial  pricing  model  are  represented  in  the  table  below  for  the  conversion  options  reflected  on  a  per  share

common stock equivalent basis.

Assumptions
Expected life (in months)
Expected volatility
Risk-free interest rate
Exercise price
Fair value per conversion option

xvii. Reclassifications

At Issuance
fair value

At Settlement
fair value

6.0-24.0 
104.2-153.8%   
0.05-0.99%   
  $
  $

3.00-$10.50 
2.70-$8.40 

0-18.0 

86.9%-142.2%   
0.01-0.72%   
  $
3.00-$7.50 
  $
2.10-$7.80 

  $
  $

Conversion options
revalued at
December 31, 2016

6.0-15.0 
84.4-94.8%
0.62-0.85%

8.40 
0.90-$1.80 

Certain prior year amounts have been reclassified to conform to our current year presentation.

xviii. Recently Issued Accounting Standards

In November 2016, the Financial Accounting Standards Board (the FASB) issued Accounting Standards Update (ASU) No. 2016-18 (ASU
2016-18). ASU 2016-18 requires that a statement of cash flows explain the change during the period in the total of cash, cash equivalents, and
amounts generally described as restricted cash or restricted cash equivalents. ASU 2016-18 is effective for fiscal years beginning after December
15, 2017. As early adoption of this amendment is permitted, the Company has adopted the update retrospectively to each period presented. The
adoption of this guidance did not have a material impact on the company’s consolidated Financial Statements.

In  March  2016,  the  FASB  issued ASU  2016-09,  which  simplifies  several  aspects  of  the  accounting  for  share-based  payment  transactions,
including the income tax consequences, classification of awards as either equity or liabilities, and classification on the statement of cash flows.
ASU  2016-09  became  effective  for  the  Company  on  January  1,  2017.  The  adoption  of  this  guidance  did  not  have  a  material  impact  on  the
Company’s consolidated Financial Statements.

In February 2016, the FASB issued ASU 2016-02, which requires an entity to recognize assets and liabilities arising from a lease for both
financing and operating leases, along with additional qualitative and quantitative disclosures. ASU 2016-02 is effective for fiscal years beginning
after  December  15,  2018,  with  early  adoption  permitted.  The  Company  is  currently  evaluating  the  effect  this  standard  will  have  on  its
consolidated Financial Statements.

In  August  2014,  the  Financial  Accounting  Standards  Board  (“FASB”)  issued  Accounting  Standards  Update  (“ASU”)  No.  2014-15,
Presentation of Financial Statements-Going Concern (Subtopic 205-40): Disclosure of Uncertainties about an Entity’s Ability to Continue as a
Going Concern. (“ASU 2015-14”). Under the new standard, management must evaluate whether there are conditions or events, considered in the
aggregate, that raise substantial doubt about the Company’s ability to continue as a going concern within one year after the date that the financial
statements are issued. This evaluation initially does not take into consideration the potential mitigating effect of management’s plans that have not
been fully implemented as of the date the financial statements are issued. When substantial doubt exists under this methodology, management
evaluates whether the mitigating effect of its plans sufficiently alleviates substantial doubt about the Company’s ability to continue as a going
concern.  The  mitigating  effect  of  management’s  plans,  however,  is  only  considered  if  both  (1)  it  is  probable  that  the  plans  will  be  effectively
implemented within one year after the date that the financial statements are issued, and (2) it is probable that the plans, when implemented, will
mitigate the relevant conditions or events that raise substantial doubt about the entity’s ability to continue as a going concern within one year after
the date that the financial statements are issued. This standard was adopted by the Company at January 1, 2017. See Note 2.

 In May 2014, the FASB issued ASU 2014-09, Revenue from Contracts with Customers (Topic 606) which amended the existing accounting
standards for revenue recognition. ASU 2014-09 establishes principles for recognizing revenue upon the transfer of promised goods or services to
customers,  in  an  amount  that  reflects  the  expected  consideration  received  in  exchange  for  those  goods  or  services.  In  July  2015,  the  FASB
deferred  the  effective  date  for  annual  reporting  periods  beginning  after  December  15,  2017  (including  interim  reporting  periods  within  those
periods). The amendments may be applied retrospectively to each prior period (full retrospective) or retrospectively with the cumulative effect

 
 
 
 
 
   
   
   
   
 
 
 
 
 
 
   
   
   
   
 
 
 
   
   
 
   
   
   
   
   
 
 
 
 
 
 
 
 
recognized as of the date of initial application (modified retrospective). The Company will adopt ASU 2014-09 in the first quarter of 2018 and
apply  the  modified  retrospective  approach.  The  Company’s  primary  source  of  revenues  is  from  instrument  sales  which  are  considered  distinct
performance obligations and are recognized upon shipment, the Company does not expect the impact on its consolidated financial statements to
be material.

- 62 -

 
(4) Property and Equipment, net

Property and equipment as of December 31, 2017 and 2016 consisted of the following components:

Laboratory and manufacturing equipment
Office equipment
Leasehold improvements
PCT collaboration, demonstration and leased systems
Total property and equipment
Less accumulated depreciation
Net book value

December 31,

2017

2016

  $

  $

240,670    $
173,312   
8,117   
461,858   
883,957   
(861,295)  

22,662    $

226,326 
165,832 
8,117 
461,858 
862,133 
(852,720)
9,413 

Depreciation expense for the years ended December 31, 2017 and 2016 was $8,576 and $17,939, respectively.

(5) Intangible Assets

Intangible  assets  as  of  December  31,  2017  reflect  the  purchase  price  attributable  to  patents  received  in  connection  with  the  acquisition  of
assets of BaroFold Corp. Acquired BaroFold patents are being amortized to expense on a straight line basis at the rate of $80,000 per year over
their estimated remaining useful lives of approximately 9 years. The estimated aggregate amortization expense for each of the five succeeding
fiscal years is $80,000 annually. We performed a review of our intangible assets for impairment. When impairment is indicated, any excess of
carrying value over fair value is recorded as a loss. An impairment analysis of intangible assets was performed as of December  31,  2017.  We
have concluded that there is no impairment of intangible assets. Intangible assets at December 31, 2017 and 2016 consisted of the following:

BaroFold Patents
Less accumulated amortization
Net book value

December 31,

2017

2016

  $

  $

750,000    $

-   

750,000    $

- 
- 
- 

Amortization expense for each of the years ended December 31, 2017 and 2016 was $0.

(6) Retirement Plan

We  provide  all  of  our  employees  with  the  opportunity  to  participate  in  our  retirement  savings  plan.  Our  retirement  savings  plan  has  been
qualified  under  Section  401(k)  of  the  Internal  Revenue  Code.  Eligible  employees  are  permitted  to  contribute  to  the  plan  through  payroll
deductions  within  statutory  limitations  and  subject  to  any  limitations  included  in  the  plan.  During  2017  and  2016  we  contributed  $13,587  and
$22,627, respectively, in the form of discretionary Company-matching contributions.

- 63 -

 
 
 
 
 
 
 
 
 
   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
   
 
 
 
 
 
 
 
 
(7) Income Taxes

Tax positions must meet a “more likely than not”  recognition  threshold  at  the  effective  date  to  be  recognized. At  December  31,  2017  and
2016,  the  Company  did  not  have  any  uncertain  tax  positions.  No  interest  and  penalties  related  to  uncertain  tax  positions  were  accrued  at
December 31, 2017 and 2016. Our tax returns for fiscal years 2014, 2015 and 2016 are open to examination.

We did not record an income tax benefit or provision for the years ended December 31, 2017 and 2016.

Significant items making up the deferred tax assets and deferred tax liabilities as of December 31, 2017 and 2016 are as follows:

Long term deferred taxes:
Inventories
Accounts receivable allowance
Other accruals
Accelerated tax depreciation
Non-cash, stock-based compensation, nonqualified
Impairment loss on investment
Goodwill and intangibles
Operating loss carry forwards and tax credits
Less: valuation allowance
Total net deferred tax assets

2017

2016

  $

49,067    $

-   
75,992   
6,945   
606,020   
103,748   
-   
12,196,765   
(13,038,537 )  

  $

-    $

7,856 
17,253 
33,399 
14,582 
711,676 
146,782 
- 
13,561,012 
(14,492,560)
- 

A  valuation  allowance  is  established  if  it  is  more  likely  than  not  that  all  or  a  portion  of  the  deferred  tax  asset  will  not  be  realized.
Accordingly,  a  valuation  allowance  was  established  in  2017  and  2016  for  the  full  amount  of  our  deferred  tax  assets  due  to  the  uncertainty  of
realization. We believe based on our projection of future taxable operating income for the foreseeable future, it is more likely than not that we
will not be able to realize the benefit of the deferred tax asset at December 31, 2017.

On December 22, 2017, the U.S. Tax Cuts and Jobs Act (the "Tax Reform Act") was signed into law by President Trump. The Tax Reform
Act significantly revised the U.S. corporate income tax regime by, among other things, lowering the U.S. corporate tax rate from 35% to 21%
effective January 1, 2018. Under ASC 740, the tax effects of changes in tax laws must be recognized in the period in which the law was enacted.
ASC 740 also requires deferred tax assets and liabilities to be measured at the enacted tax rate expected to apply when temporary differences are
to be realized or settled. The Company re-measured its deferred tax assets and liabilities at the 21% federal corporate tax rate. The remeasurement
resulted in no change in the Company’s recorded tax provision, as the remeasurement of the company's deferred tax assets and liabilities resulted
in a reduction of approximately $5,843,000 which was fully offset by a change in the Company's valuation allowance.

We  have  net  operating  loss  carry-forwards  for  federal  income  tax  purposes  of  $38,690,000  as  of  December  31,  2017.  Included  in  these
numbers are loss carry-forwards that were obtained through the acquisition of BioSeq, Inc. and are subject to Section 382 NOL limitations. These
net operating loss carry-forwards expire at various dates from 2018 through 2038.

We  have  net  operating  loss  carry-forwards  for  state  income  tax  purposes  of  approximately  $31,735,000  at  December  31,  2017.  These  net

operating loss carry-forwards expire at various dates from 2030 through 2038.

We have research and development tax credit carry-forwards for federal income tax purposes of approximately $1,089,000 as of December
31, 2017 and research and development tax credit carry-forwards for state income tax purposes of approximately $232,000 as of December 31,
2017. The federal credit carry-forwards expire at various dates from 2018 through 2038. The state credit carry-forwards expire at various dates
from 2023 through 2033.

In addition, we have federal alternative minimum tax credit carry-forwards for federal income tax purposes of approximately $217,000 as of

December 31, 2017. These credits do not expire.

- 64 -

 
 
 
 
 
 
 
 
   
 
 
 
    
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Our effective income tax (benefit) provision rate was different than the statutory federal income tax (benefit) provision rate as follows for the

years ended December 31:

Federal tax provision rate
Permanent differences
State tax expense
Refundable AMT and R&D tax credit
Net operating loss carry back
Valuation allowance
Effective income tax provision

(8) Commitments and Contingencies

Operating Leases

2017

2016

35%  
(0)% 
0%  
0%  
0%  
(35)% 
0%  

34%
24%
0%
0%
0%
(58)%
0%

Our  corporate  office  is  currently  located  at  14  Norfolk Avenue,  South  Easton,  Massachusetts  02375.  We  are  currently  paying  $6,950  per
month, on a lease extension, signed on December 29, 2017, that expires December 31, 2018, for our corporate office. We expanded our space to
include offices, warehouse and a loading dock on the first floor starting May 1, 2017 with a monthly rent increase already reflected in the current
payments.

We extended our lease for our space in Medford, MA to December 30, 2020. The lease requires monthly payments of $6,912.75 subject to
annual  cost  of  living  increases.  The  lease  shall  be  automatically  extended  for  additional  three  years  unless  either  party  terminates  at  least  six
months prior to the expiration of the current lease term.

Following is a schedule by years of future minimum rental payments required under operating leases with initial or remaining non-cancelable

lease terms in excess of one year as of December 31, 2017:

2018
2019
2020
Thereafter
Total minimum payments required

Royalty Commitments

BioMolecular Assays, Inc.

  $

  $

166,353 
82,953 
82,953 
- 
332,259 

In 1996, we acquired our initial equity interest in BioSeq, Inc., which at the time was developing our original pressure cycling technology.
BioSeq,  Inc.  acquired  its  pressure  cycling  technology  from  BioMolecular  Assays,  Inc.  under  a  technology  transfer  and  patent  assignment
agreement. In 1998, we purchased all of the remaining outstanding capital stock of BioSeq, Inc., and at such time, the technology transfer and
patent assignment agreement was amended to require us to pay BioMolecular Assays, Inc., a 5% royalty on our sales of products or services that
incorporate or utilize the original pressure cycling technology that BioSeq, Inc. acquired from BioMolecular Assays, Inc. We are also required to
pay  BioMolecular Assays,  Inc.  5%  of  the  proceeds  from  any  sale,  transfer  or  license  of  all  or  any  portion  of  the  original  pressure  cycling
technology.  These  payment  obligations  terminated  on  March  7,  2016.  During  the  year  ended  December  31,  2016,  we  incurred  approximately
$6,963 in royalty expense associated with our obligation to BioMolecular Assays, Inc.

In  connection  with  our  acquisition  of  BioSeq,  Inc.,  we  licensed  certain  limited  rights  to  the  original  pressure  cycling  technology  back  to
BioMolecular Assays, Inc. This license is non-exclusive and limits the use of the original pressure cycling technology by BioMolecular Assays,
Inc.  solely  for  molecular  applications  in  scientific  research  and  development  and  in  scientific  plant  research  and  development.  BioMolecular
Assays, Inc. is required to pay us a royalty equal to 20% of any license or other fees and royalties, but not including research support and similar
payments, it receives in connection with any sale, assignment, license or other transfer of any rights granted to BioMolecular Assays, Inc. under
the license. BioMolecular Assays, Inc. was required to pay us these royalties until the expiration in March 2016 of the patents held by BioSeq,
Inc. since 1998. We have not received any royalty payments from BioMolecular Assays, Inc. under this license.

- 65 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Battelle Memorial Institute

In  December  2008,  we  entered  into  an  exclusive  patent  license  agreement  with  the  Battelle  Memorial  Institute  (“Battelle”).  The  licensed
technology  is  the  subject  of  a  patent  application  filed  by  Battelle  in  2008  and  relates  to  a  method  and  a  system  for  improving  the  analysis  of
protein samples, including through an automated system utilizing pressure and a pre-selected agent to obtain a digested sample in a significantly
shorter  period  of  time  than  current  methods,  while  maintaining  the  integrity  of  the  sample  throughout  the  preparatory  process.  In  addition  to
royalty payments on net sales on “licensed products,” we are obligated to make minimum royalty payments for each year that we retain the rights
outlined in the patent license agreement and we are required to have our first commercial sale of the licensed products within one year following
the issuance of the patent covered by the licensed technology. After re-negotiating the terms of the contract in 2013, the minimum annual royalty
was $1,200 in 2014 and $2,000 in 2015; the minimum royalties are $3,000 in 2016, $4,000 in 2017 and $5,000 in 2018 and each calendar year
thereafter during the term of the agreement.

Target Discovery Inc.

In March 2010, we signed a strategic product licensing, manufacturing, co-marketing, and collaborative research and development agreement
with Target Discovery Inc. (“TDI”), a related party. Under the terms of the agreement, we have been licensed by TDI to manufacture and sell a
highly innovative line of chemicals used in the preparation of tissues for scientific analysis (“TDI reagents”). The TDI reagents were designed for
use in combination with our pressure cycling technology. The companies believe that the combination of PCT and the TDI reagents can fill an
existing need in life science research for an automated method for rapid extraction and recovery of intact, functional proteins associated with cell
membranes in tissue samples. We did not incur any royalty obligation under this agreement in 2017 or 2016.

In April 2012, we signed a non-exclusive license agreement with TDI to grant the non-exclusive use of our pressure cycling technology. We
recorded  $30,000  and  $20,000  of  minimum  royalty  income  in  2017  and  2016,  respectively.  We  executed  an  amendment  to  this  agreement  on
October 1, 2016 wherein we agreed to pay a monthly fee of $1,400 for the use of a lab bench, shared space and other utilities, and $2,000 per day
for technical support services as needed.

Severance and Change of Control Agreements

Each  of  Mr.  Schumacher,  and  Drs.  Ting,  Lazarev,  and  Lawrence,  executive  officers  of  the  Company,  are  entitled  to  receive  a  severance
payment if terminated by us without cause. The severance benefits would include a payment in an amount equal to one year of such executive
officer’s annualized base salary compensation plus accrued paid time off. Additionally, the officer will be entitled to receive medical and dental
insurance coverage for one year following the date of termination.

Each of these executive officers, other than Mr. Schumacher, is entitled to receive a change of control payment in an amount equal to one
year of such executive officer’s annualized base salary compensation, accrued paid time off, and medical and dental coverage, in the event of a
change  of  control  of  the  Company.  In  the  case  of  Mr.  Schumacher,  this  payment  would  be  equal  to  two  years  of  annualized  base  salary
compensation,  accrued  paid  time  off,  and  two  years  of  medical  and  dental  coverage.  The  severance  payment  is  meant  to  induce  the
aforementioned executives to remain in the employ of the Company, in general; and particularly in the occurrence of a change in control, as a
disincentive to the control change.

(9) Convertible Debt and Other Debt

Senior Secured Convertible Debentures and Warrants

We entered into Subscription Agreements (the “ Subscription Agreement”) with various individuals (each, a “Purchaser”)  between  July  23,
2015  and  March  31,  2016,  pursuant  to  which  the  Company  sold  Senior  Secured  Convertible  Debentures  (the  “Debentures”)  and  warrants  to
purchase  shares  of  common  stock  equal  to  50%  of  the  number  of  shares  issuable  pursuant  to  the  subscription  amount  (the  “Warrants”)  for  an
aggregate purchase price of $6,329,549 (the “Purchase Price”).

- 66 -

 
 
 
 
 
 
 
 
 
 
 
 
 
The  Company  issued  a  principal  aggregate  amount  of  $6,962,504  in  Debentures  which  includes  a  10%  original  issue  discount  on  the
Purchase Price. The Debenture does not accrue any additional interest during the first year it is outstanding but accrues interest at a rate equal to
10% per annum for the second year it is outstanding. The Debenture has a maturity date of two years from issuance. The Debenture is convertible
any  time  after  its  issuance  date.  The  Purchaser  has  the  right  to  convert  the  Debenture  into  shares  of  the  Company’s  common  stock  at  a  fixed
conversion price equal to $8.40 per share, subject to applicable adjustments. In the second year that the Debenture is outstanding, any interest
accrued shall be payable quarterly in either cash or common stock, at the Company’s discretion.

On various dates for the year ended December 31, 2017, the Company issued 61,307 shares of common stock based on the 10-day VWAP
prior to quarter end to holders of the Debentures in payment of the quarterly interest accrued from the Debentures first anniversary date through
June 30, 2017 for an aggregate amount of $483,054. We recognized a $218,452 gain on extinguishment of debt by calculating the difference of
the shares valued on the issuance date and the amount of accrued interest through June 30, 2017.

At any time after the Issuance Date, the Company has the option, subject to certain conditions, to redeem some or all of the then outstanding
principal amount of the Debenture for cash in an amount equal to the sum of (i) 120% of the then outstanding principal amount of the Debenture,
(ii) accrued but unpaid interest and (iii) any liquidated damages and other amounts due in respect of the Debenture.

On September 11, 2017, we notified Debenture holders that their Debentures will be extended 180 days beyond the original maturity date as
permitted in the Debenture agreement. We will continue to pay interest on the Debentures until the extended maturity date. We accounted for the
Debenture extensions as debt modifications and not extinguishment of debt since the changes in fair value are not substantial in accordance with
ASC 470-50. We started amortizing the remaining unamortized discount as of September 11, 2017 over the new term, which extends 180 days
beyond the original maturity date.

The Company issued warrants exercisable into a total of 376,759 shares of our common stock. The Warrants issued in this transaction are
immediately exercisable at an exercise price of $12.00 per share, subject to applicable adjustments including full ratchet anti-dilution in the event
that we issue any securities at a price lower than the exercise price then in effect. The Warrants have an expiration period of five years from the
original issue date. The Warrants are subject to adjustment for stock splits, stock dividends or recapitalizations and also include anti-dilution price
protection for subsequent equity sales below the exercise price.

Subject to the terms and conditions of the Warrants, at any time commencing six months from the Final Closing, the Company has the right
to call the Warrants for cancellation if the volume weighted average price of its Common Stock on the OTCQB (or other primary trading market
or exchange on which the Common Stock is then traded) equals or exceeds three times the per share exercise price of the Warrants for 15 out of
20 consecutive trading days.

In connection with the Subscription Agreement and Debenture, the Company entered into Security Agreements with the Purchasers whereby
the Company agreed to grant to Purchasers an unconditional and continuing, first priority security interest in all of the assets and property of the
Company to secure the prompt payment, performance and discharge in full of all of Company’s obligations under the Debentures, Warrants and
the other Transaction Documents.

The Company determined that the conversion feature of the Debentures met the definition of a liability in accordance with ASC 815-40 and
therefore bifurcated the conversion feature on each debt agreement and accounted for it as a derivative liability. The fair value of the conversion
feature was accounted for as a note discount and is amortized to interest expense over the life of the loan. The fair value of the conversion feature
was reflected in the conversion option liability line in the consolidated balance sheet as of December 31, 2016.

The proceeds from these convertible debts were allocated between the host debt instrument and the convertible option based on the residual
method. The estimated fair value of the convertible option was determined using a binomial formula, resulting in allocations to the convertible
option and accounted for as a liability in the Company’s consolidated balance sheet. In accordance with the provisions of ASC 815-40, the gross
proceeds are offset by debt discounts, which are amortized to interest expense over the expected life of the debt.

- 67 -

 
 
 
 
 
 
 
 
 
 
 
ASC  470-20  states  that  the  proceeds  from  the  issuance  of  debt  with  detachable  stock  warrants  should  be  allocated  between  the  debt  and
warrants on the basis of their relative fair market values. The debt discount will be amortized to interest expense over the two-year term of these
loans.  We  amortized  $3,740,746  of  the  debt  discount  to  interest  expense  in  2016.  The  warrants  issued  in  connection  with  the  convertible
debentures  are  classified  as  warrant  derivative  liabilities  because  the  warrants  are  entitled  to  certain  rights  in  subsequent  financings  and  the
warrants  contain  “down-round  protection”  and  therefore,  do  not  meet  the  scope  exception  for  treatment  as  a  derivative  under  ASC  815,
Derivatives and Hedging, (“ASC 815”). Since “down-round protection” is not an input into the calculation of the fair value of the warrants, the
warrants cannot be considered indexed to the Company’s own stock which is a requirement for the scope exception as outlined under ASC 815.
The estimated fair value of the warrants was determined using the binomial model, resulting in an allocation of $2,847,624  to the total warrants
out of the gross proceeds of $6,329,549. The fair value will be affected by changes in inputs to that model including our stock price, expected
stock price volatility, the contractual term, and the risk-free interest rate.

We early adopted ASU 2017-11 and applied the guidance to derivative accounting. We reclassified the fair value of the warrant derivative

liabilities to stockholders’ equity when we adopted ASU 2017-11.

Other convertible notes

On May 13, 2016, one lender converted an outstanding note issued on April 28, 2015 and the related accrued interest totaling $117,837

to 14,028 common shares. As of December 31, 2017, the outstanding balance on the note was zero.

On May 24, 2016, we sold an additional convertible note for $107,000 with warrants to purchase 1,667 shares of common stock at an
exercise  price  of  $16.50  per  share.  The  purchaser  has  the  right  to  convert  the  notes  into  shares  of  the  Company’s  common  stock  at  a  fixed
conversion price equal to $13.50 per share, subject to applicable adjustments. The estimated fair value of the warrants was determined using the
binomial model, resulting in an allocation of $12,406 to the total warrants and the recognition of a beneficial conversion feature of $7,962, both of
which were recorded as a discount to the note. We evaluated the convertible note and warrants for derivative liability treatment and determined
that these instruments do not include certain rights such as price protection like our previous debt financings. Accordingly, we concluded that this
financing arrangement did not qualify for derivative accounting treatment. The loan was paid in full in fiscal year 2016.

On June 14, 2016, we sold an additional convertible note for $115,000 and issued 1,023 common shares to compensate the lender. On
July 1, 2016, the note was modified to increase the principal amount to $200,000 and we received the remaining proceeds of $85,000 on the same
date and issued 1,144 common shares as compensation to the lender. The lender has the right to convert the note into shares of the Company’s
common stock at fixed conversion price equal to $13.50 per share, subject to applicable adjustments. We valued the total 2,167 common shares
using  the  stock  prices  at  the  respective  dates  the  note  proceeds  were  received  and  recorded  the  relative  fair  value  of  the  shares  amounting  to
$26,000 as a debt discount to be amortized over the term of the loan. We then computed the effective conversion price of the note, noting that no
beneficial conversion feature exists. We also evaluated the convertible note for derivative liability treatment and determined that the instrument
does  not  include  certain  rights  such  as  price  protection  like  our  previous  debt  financing.  Accordingly,  we  concluded  that  this  financing
arrangement did not qualify for derivative accounting treatment. The loan was paid in full in fiscal year 2017.

On July 29, 2016, we sold an additional convertible note for $100,000 and issued 1,084 common shares to compensate the lender. The
lender has the right to convert the notes into shares of the Company’s common stock at a fixed conversion price equal to $13.50 per share, subject
to  applicable  adjustments.  The  proceeds  were  allocated  between  the  convertible  note  and  shares  of  common  stock  based  on  their  relative  fair
values.  The  relative  fair  values  of  the  convertible  note  and  the  common  shares  was  $87,241  and  $12,759,  respectively.  We  then  computed  the
effective conversion price of the note, noting that the convertible debt gave rise to a beneficial conversion feature (BCF) of $12,759. The sum of
the relative fair value of the common shares and the BCF of $25,518 was recorded as a debt discount to be amortized over the term of the loan.
We also evaluated the convertible note for derivative liability treatment and determined that the instruments does not include certain rights such as
price  protection  like  our  previous  debt  financings. Accordingly,  we  concluded  that  this  financing  arrangements  did  not  qualify  for  derivative
accounting treatment. The loan was paid in full in fiscal year 2017.

On September 15, 2016, we sold an additional convertible note for $500,000 and issued 6,666 common shares to compensate the lender. The
lender has the right to convert the notes into shares of the Company’s common stock at a fixed conversion price equal to $13.50 per share, subject
to  applicable  adjustments.  The  convertible  note  includes  an  original  issue  discount  of  $40,541  and  is  subject  to  a  one-time  interest  of  9%  or
$45,000 which was recorded as a debt discount and amortized over the term of the loan. The proceeds were allocated between the convertible
note and shares of common stock based on their relative fair values. The relative fair value of the convertible note was $434,028. The allocation
of the gross proceeds to the shares of common stock was $65,972 and recorded as a debt discount to be amortized over the term of the loan. We
then computed the effective conversion price of the note, noting that no beneficial conversion feature exists. We also evaluated the convertible
note for derivative liability treatment and determined that the instrument does not include certain rights such as price protection like our previous
debt  financings. Accordingly,  we  concluded  that  this  financing  arrangement  did  not  qualify  for  derivative  accounting  treatment.  The  loan  was
paid in full in fiscal year 2017.

On various dates during the quarter ended December 31, 2017, the Company issued convertible notes for net proceeds of $1,755,850 with the
following  terms:  a)  maturity  ranging  from  3  to  12  months;  b)  annual  interest  rates  ranging  from  5%  to  12%;  c)  convertible  to  the  Company’s
common stock at issuance at a fixed rate of $7.50 or convertible at variable conversion rates either after 6 months after issuance or in the event of
a default. Certain of these notes were issued with shares or warrants which were fair valued at issuance dates. The relative fair values of the shares
or  warrants  issued  with  the  notes  were  recorded  as  a  debt  discount  and  amortized  over  the  term  of  the  notes.  We  then  computed  the  effective
conversion price of the notes, noting that no beneficial conversion feature exists. We also evaluated the convertible notes for derivative liability
treatment and determined that the notes did not qualify for derivative accounting treatment as of December 31, 2017.

- 68 -

 
 
 
 
 
 
 
 
 
 
 
The specific terms of the convertible notes and outstanding balances as of December 31, 2017 are listed in the tables below.

Convertible Notes

Loan

Amount    

Outstanding
Balance

Original
Issue
Discount   

Interest
Rate

Conversion
Price(Convertible
at Inception
Date)

Deferred
Finance
Fees

Discount
related 
to fair 
value of
conversion
feature 
and
warrants/shares 

$2,180,000    $ 2,180,000    $218,0002 

  10%3  

  $

8.40 

  $ 388,532 

  $

2,163,074 

  1,100,000   

1,100,000   

  110,0002 

  10%3  

  $

8.40 

185,956 

1,022,052 

150,000   

150,000   

  15,0002 

  10%3  

  $

8.40 

26,345 

140,832 

30,000   

30,000   

3,0002 

  10%3  

  $

50,000   

50,000   

5,0002 

  10%3  

  $

8.40 

8.40 

5,168 

8,954 

26,721 

49,377 

250,000   

250,000   

  25,0002 

  10%3  

  $

8.40 

43,079 

222,723 

50,000   

50,000   

5,0002 

  10%3  

  $

8.40 

8,790 

46,984 

215,000   

215,000   

  21,5002 

  10%3  

  $

8.40 

38,518 

212,399 

200,000   

200,000   

  20,0002 

  10%3  

  $

8.40 

37,185 

200,000 

170,000   

170,000   

  17,0002 

  10%3  

  $

8.40 

37,352 

170,000 

360,000   

360,000   

  36,0002 

  10%3  

  $

8.40 

75,449 

360,000 

55,000   

55,000   

5,5002 

  10%3  

  $

8.40 

11,714 

55,000 

100,000   

100,000   

  10,0002 

  10%3  

  $

8.40 

20,634 

100,000 

100,000   

100,000   

  10,0002 

  10%3  

  $

8.40 

24,966 

50,000   

50,000   

5,0002 

  10%3  

  $

8.40 

9,812 

80,034 

40,188 

300,000   

300,000   

  30,0002 

  10%3  

  $

8.40 

60,887 

239,113 

200,000   

200,000   

  20,0002 

  10%3  

  $

8.40 

43,952 

156,048 

125,000   

125,000   

  12,5002 

  10%3  

  $

8.40 

18,260 

106,740 

360,000   

360,000   

  36,0002 

  10%3  

  $

8.40 

94,992 

265,008 

106,667   

106,667   

  10,6672 

  10%3  

  $

8.40 

15,427 

91,240 

177,882   

177,882   

  17,7882 

  10%3  

  $

8.40 

2,436 

175,446 

40,000   

40,000   

35,000   

85,000   

100,000   

500,000   

85,000   

-   

-   

-   

-   

-   

-   

  12%  

  $

-   

  12%  

  $

-   

  12%  

  $

-   

  12%  

  $

-   

  12%  

  $

-   

  85,541   

  9%  

  $

13.50 

13.50 

13.50 

13.50 

13.50 

13.50 

- 

- 

- 

- 

- 

- 

-   

-   

  5%  

- 

4,250 

3,680 

3,899 

3,373 

15,048 

25,518 

65,972 

- 

Inception
Date

  Term  
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
30
months1 
6
months  
6
months  
6
months  
6
months  
6
months  
8
months  
12
months  
12

July 22, 2015  
September 25,
2015
October 2,
2015
October 6,
2015
October 14,
2015
November 2,
2015
November 10,
2015
November 12,
2015
November 20,
2015
December 4,
2015
December 11,
2015
December 18,
2015
December 31,
2015
January 11,
2016
January 20,
2016
January 29,
2016
February 26,
2016
March 10,
2016
March 18,
2016
March 24,
2016
March 31,
2016

June 15, 2016  

June 17, 2016  

June 22, 2016  

July 6, 2016

July 29, 2016  
September 15,
2016
October 11,
2017
October 20,

 
 
 
 
   
 
 
 
 
 
 
 
   
   
 
 
 
   
   
 
 
 
 
   
   
 
 
 
 
   
   
 
 
 
   
   
 
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
 
   
   
 
 
 
   
   
 
 
 
   
   
 
 
 
 
 
 
   
   
2017(4)
October 25,
2017
October 27,
2017
November 13,
2017
November 22,
2017
November 28,
2017
November 29,
2017
November 30,
2017
December 5,
2017
December 6,
2017
December 11,
2017
December 19,
2017
December 28,
2017
December 29,
2017

  months  
6
months  
12
months  
9
months   
12
months  
10
months  
6
months  
3
months   
3
months  
4
months   
6
months  
6
months  
6
months  
12
months  

150,000   

150,000   

103,000   

103,000   

-   

-   

  12%  

  5%  

  $

7.50 

170,000   

170,000   

-   

  5%  

7,500 

3,000 

4,250 

- 

- 

380,000   

380,000   

  15,200   

  8%  

  $

7.50 

15,200 

100,000   

100,000   

  10,000   

  5%  

103,000   

103,000   

3,000   

  12%  

150,000   

150,000   

-   

  15%  

  $

50,000   

50,000   

  8%  

  $

52,500   

52,500   

-   

  10%  

  $

100,000   

100,000   

-   

  10%  

  $

130,000   

130,000   

1,500   

  5%  

110,000   

110,000   

1,500   

  5%  

- 

- 

7.50 

7.50 

7.50 

7.50 

- 

- 

55,000   

55,000   

-   

  15%  

  $

7.50 

2,000 

- 

- 

2,500 

- 

6,500 

5,500 

5,000 

- 

10,000 

46,274 

- 

- 

15,200 

- 

- 

6,460 

5,775 

- 

105,000   

-   
$8,973,049    $ 8,088,049    $749,696   

105,000   

  5%  

- 

5,000 
  $1,219,108 

  $

- 
6,124,178 

1) The loan term was extended by 180 days and further extended on January 15, 2018 by 60 days to repay in common stock. The July 22,

2015 Debentures of $2,180,000 is currently past due as of March 19, 2018.

2. The original issue discount is reflected in the first year.
3. The annual interest started accruing in the second year.
4. On October 20, 2017, Company issued to EMA Financial, LLC a 5% one year convertible note in the amount of $150,000, less $7,500
from OID and fees. The note is convertible at $7.50 per share, provided however, if the Company fails to comply with Section 1.9 of the note then
the  conversion  price  shall  be  65%  of  the  lowest  sale  price  for  the  Common  Stock  on  the  Principal  Market  during  the  twenty  (20)  consecutive
Trading Days immediately preceding the Conversion Date or the closing bid price, whichever is lower.

As of December 31, 2017, a total of approximately $291,000 convertible debentures were purchased by related parties who were members of

the Company’s Board of Directors and management and their family members.

Deferred finance fees included cash commissions amounting to $621,500 and the fair value of the 2,101,786 warrants issued to the placement
agent  amounting  to  $536,908.  For  the  year  ended  December  31,  2017,  the  Company  recognized  amortization  expense  related  to  the  debt
discounts indicated above of $1,955,193. The unamortized debt discounts as of December 31, 2017 related to the convertible debentures and other
convertible notes amounted to $433,228.

- 69 -

 
 
 
   
   
 
 
 
 
 
 
   
   
  
 
 
 
 
 
 
   
   
 
 
 
   
   
 
 
 
 
 
   
   
 
 
 
 
 
 
   
   
 
 
 
 
   
   
 
 
 
 
    
   
  
   
  
 
 
 
 
   
   
 
 
 
 
   
   
 
 
 
 
 
 
   
   
 
 
 
 
 
 
   
   
 
 
 
 
   
   
 
 
 
 
 
 
   
   
 
 
 
 
 
  
 
 
 
  
 
 
 
 
Revolving Note Payable

On October 28, 2016, an accredited investor (the “Investor”) purchased from us a promissory note in the aggregate principal amount of up to
$2,000,000 (the “Revolving Note”) due and payable on the earlier of October 28, 2017 (the “Maturity Date”) or on the seventh business day after
the closing of a Qualified Offering (as defined in the Revolving Note). Although the Revolving Note is dated October 26, 2016, the transaction
did not close until October 28, 2016, when we received its initial $250,000 advance pursuant to the Revolving Note. As a result, on the same day
and pursuant to the Revolving Note, we issued to the Investor a Common Stock Purchase Warrant to purchase 20,834 shares of our common stock
at an exercise price per share equal to $12.00 per share. The Investor is obligated to provide us with advances of $250,000 under the Revolving
Note, but the Investor shall not be required to advance more than $250,000 in any individual fifteen (15) day period and no more than $500,000 in
the  thirty  (30)  day  period  immediately  following  the  date  of  the  initial  advance.  We  received  $3,500,000  pursuant  to  the  Revolving  Note  as
amended  of  which  $2,070,000  net  proceeds  was  received  in  2017  and  we  issued  to  the  Investor  warrants  to  purchase  291,667  shares  of  our
Common Stock at an exercise price per share equal to $12.00 per share. The terms of the Warrants are identical except for the exercise date, issue
date, and termination date which are based on the advance date.

The Revolving Note was amended on May 2, 2017 to increase the aggregate principal amount to $3,000,000, to issue 16,667 shares of our
Common Stock to the Investor, to decrease the exercise price per share of the warrants to the lower of (i) $12.00 or (ii) the per share purchase
price of the shares of our Common Stock sold in the Qualified Offering, and to change the references in the Revolving Note from “the six (6)
month anniversary of October 28, 2016” to “July 25, 2017.” The fair value of the 16,667 shares issued of $149,018 was accounted for as a note
discount and are amortized to interest expense over the life of the loan. We evaluated the accounting impact of the Revolving Note amendment
and deemed that the amendment did not have a material impact on our consolidated financial statements.

The Revolving Note was further amended on August 18, 2017 to increase the aggregate principal amount to $3,500,000 with all other terms

unchanged.

In the event that a Qualified Offering had occurred after July 25, 2017, but prior to the Maturity Date, within seven (7) Business Days of the
closing of the Qualified Offering, the Company was to pay a cash fee equal to five percent (5%) of the total outstanding amount owed by the
Company  to  the  Holder  as  of  the  closing  date  of  the  Qualified  Offering  or,  at  the  option  of  the  Company,  issue  to  the  Holder  a  number  of
restricted shares of the Company’s common stock equal to (x) five percent (5%) of the total outstanding amount owed by the Company to the
Holder  as  of  the  closing  date  of  the  Qualified  Offering  divided  by  (y)  the  purchase  price  provided  by  the  documents  governing  the  Qualified
Offering. A  Qualified Offering means the completion of a public offering of the Company’s securities pursuant to which the Company receives
aggregate  gross  proceeds  of  at  least  Seven  Million  United  States  Dollars  (US$7,000,000)  in  consideration  of  the  purchase  of  its  securities  and
resulting  in,  pursuant  to  the  effectiveness  of  the  registration  statement  for  such  offering,  the  Company’s  common  stock  being  traded  on  the
NASDAQ Capital Market, NASDAQ Global Select Market or the New York Stock Exchange. A Qualified Offering did not occur on or prior to
the Maturity Date.

In the event that a Qualified Offering had not occurred after July 25, 2017, but prior to the Maturity Date, within seven (7) Business Days of
the closing of the Qualified Offering, the Company shall pay a cash fee equal to five percent (5%) of the total outstanding amount owed by the
Company to the Holder or, at the option of the Company, issue to the Holder a number of restricted shares of the Company’s common stock equal
to (x) five percent (5%) of the total outstanding amount owed by the Company to the Holder as of the Maturity Date divided by (y) the VWAP of
the Company’s common stock for the last ten trading days preceding the Maturity Date. A Qualified Offering did not occur on or prior to the
Maturity Date.

Interest on the principal balance of the Revolving Note shall be paid in full on the Maturity Date, unless otherwise paid prior to the Maturity
Date.  Interest  shall  be  assessed  as  follows:  (i)  a  one-time  interest  of  10%  on  all  principal  amounts  advanced  prior  to April  28,  2017;  (ii)  the
foregoing and 4% on any amount remaining outstanding if the principal amount is repaid between April 28, 2017 and July 28, 2017; or (iii) both
of the foregoing and 4% on any amount remaining outstanding if the principal amount is repaid between July 28, 2017 and October 28, 2017.

Broker fees amounting to $296,500, the one-time interest of $350,000 and the relative fair value of the 291,667 warrants issued to the Investor
amounting  to  $1,148,275  were  recorded  as  debt  discounts  and  amortized  over  the  term  of  the  revolving  note.  The  unamortized  debt  discounts
related to the Revolving Note were fully amortized as of December 31, 2017.

The Revolving Note was still outstanding as of December 31, 2017 and is currently past due. We continue to accrue interest on the note.

The following table provides a summary of the changes in convertible debt and revolving note payable, net of unamortized discounts, during

2017:

Balance at January 1,
Adjustment due to ASU 2017-11
Issuance of convertible debt, face value
Deferred financing cost
Debt discount related to one-time interest charge
Debt discount from incentive shares to increase the Revolving Note aggregate principal limit
Debt discount from shares and warrants issued with the notes
Payments
Accretion of interest and amortization of debt discount to interest expense through December 31,
Balance at December 31,
Less: current portion
Convertible debt, long-term portion

- 70 -

  $

  $

2017

5,273,937 
923,468 
4,093,500 
(267,650)
(225,000)
(150,000)
(750,705)
(925,541)
3,815,767 
11,787,776 
11,787,776 
- 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Other Notes

On January 20, 2016 we borrowed $50,000 from an individual with no interest or fees. We paid back the loan in March 2016.

On February  8,  2016  we  signed  a  Merchant Agreement  with  a  lender.  Under  the  agreement  we  received  $100,000  in  exchange  for  third
position rights to all customer receipts until the lender is paid $129,900, which is collected at the rate of $927 per business day. The Company
paid $2,000 in fees in connection with this loan. We received an additional $125,000  in  June  2016  under  the  existing  Merchant Agreement  of
which $48,420 was used to pay off the prior loan. The lender provided an additional $70,000 on August 16, 2016. As of December 31, 2017, the
outstanding balance on this note was zero.

On  May  9,  2016  we  signed  a  promissory  note  with  a  lender.  Under  the  agreement  we  received  $200,000  net  of  a  $6,000  original  issue
discount and we repaid $206,000 on August 25, 2016. In connection with this promissory note, we issued warrants exercisable into 3,333 shares
of our common stock. The warrants issued in this transaction are immediately exercisable at an exercise price of $16.50 per share. The warrants
have an expiration period of three years from the original issue date. The warrants are subject to adjustment for stock splits, stock dividends or
recapitalizations.  The  warrants  were  recorded  as  a  component  of  our  Stockholders’  Equity.  The  estimated  fair  value  of  the  warrants  was
determined  using  the  binomial  model,  resulting  in  an  allocation  of  $27,349  to  the  total  warrants  and  recorded  as  a  discount  to  the  note  to  be
amortized  over  the  term  of  the  loan.  We  evaluated  the  warrants  for  derivative  liability  treatment  and  determined  that  these  instruments  do  not
include certain rights such as price protection like our previous debt financings. Accordingly, we concluded that these instruments did not qualify
for derivative accounting treatment. In August 2016, the lender extended the maturity date of the note from August 11, 2016 to August 25, 2016.
Consequently, a penalty interest of $41,200 was added to the principal amount and settled through the issuance of 3,335 common shares. As of
December 31, 2016, the outstanding balance on this note was zero.

- 71 -

 
 
 
 
 
 
On February 15, 2017, we received six-month, non-convertible loans in the aggregate of $220,000 from two accredited investors. We agreed
to  issue  each  investor  5,667  shares  of  restricted  common  stock.  The  loans  earn  no  interest  but  carry  a  10%  original  issue  discount  fee.  We
recorded  the  fair  value  of  the  shares  amounting  to  $43,616  as  debt  discounts  that  will  be  amortized  to  interest  expense  during  the  term  of  the
loans.  We  received  a  one-month  extension  on  one  loan  and  two  one-month  extensions  on  the  other.  Each  extension  required  a  10%  fee  to  the
lender.  We  treated  these  extensions  as  loan  extinguishments  and  accordingly  wrote  off  the  original  debt  and  recorded  new  debt  to  include  the
extension fees as part of the principal amount. The extension fees of $33,000 to extend the loans were recorded as losses on extinguishment of
debt in the consolidated financial statements. Both loans were paid off entirely by October 31, 2017. We amortized the entire $63,616 of debt
discounts in the year ended December 31, 2017.

On March 14, 2017, we received an eight-month, non-convertible loan of $250,000 from a privately-held investment firm. The loan earned
an annual interest rate of 10% and included a 10% original issue discount. We also agreed to issue the investor 8,333 shares of restricted common
stock. We recorded the fair value of the shares amounting to $46,748 as a debt discount that will be amortized to interest expense during the term
of the loan. We amortized the entire $76,748 of the debt discount in the year ended December 31, 2017. In the event of default and at the option
of the holder, the loan was convertible into common stock at a 35% discount to the lowest closing stock price for the 15 trading days prior to
conversion. We paid the loan entirely by the maturity date so derivative accounting was triggered from the conversion option.

- 72 -

 
 
 
 
On  March  21,  2017,  we  received  an  eight-month,  non-convertible  loan  of  $170,000  from  an  accredited  investor.  The  loan  earns  an  annual
interest rate of 10% and includes a 10% original issue discount. We also agreed to issue the investor 5,667 shares of restricted common stock. We
recorded the fair value of the shares amounting to $35,079 as a debt discount that will be amortized to interest expense during the term of the
loan. The loan still remains outstanding as of December 31, 2017 with a balance of $170,000. The lender extended the term to December 31, 2017
and further to March 31, 2018 in exchange for a total of 9,500 shares of common stock. We amortized $44,841 of debt discounts in the year ended
December 31, 2017. The unamortized debt discount as of December 31, 2017 was $7,238.

On April 19, 2017, we received a 7-month non-convertible loan of $250,000 from a privately-held investment firm. The loan earned an annual
interest rate of 10% and included a 10% original issue discount. We agreed to issue 833 shares at closing. Until the loan was repaid, we agreed
that over the next one hundred eighty (180) days to issue 2,500 shares to the Investor every sixty (60) days for a total issuance of 8,333 shares. We
recorded the fair value of the 8,333 shares amounting to $43,687 as a debt discount that was amortized to interest expense during the term of the
loan. We amortized $68,687  of debt discounts in the year ended December 31, 2017. The unamortized debt discount as of December 31, 2017
was zero. In the event of default and at the option of the holder, the loan was convertible into common stock at a 35% discount to the lowest
closing  stock  price  for  the  15  trading  days  prior  to  conversion.  We  paid  the  loan  entirely  by  the  maturity  date  so  derivative  accounting  was
triggered from the conversion option.

On May 19, 2017, we received a 45-day non-convertible loan of $630,000 from a private investor. The loan provides guaranteed interest of
$63,000  and  has  an  origination  fee  of  $32,000.  We  paid  a  broker  $31,500  in  connection  with  this  loan.  The  unamortized  debt  discount  as  of
December 31, 2017 was zero. We used these proceeds to pay off in full our September 2016 loan of $589,189. The loan remains outstanding and
is currently past due. We continue to accrue interest at a 20% annual rate from the maturity date.

On August 1, 2017, we signed a non-convertible installment loan with a lender. Under the agreement we received a loan of $75,000 with a
weekly repayment of $3,500 until payment in full. The loan includes $18,750 representing an original issue discount, interest and fees resulting in
a total payable of $93,750. The loan remains outstanding as of December 31, 2017 with a balance of approximately $16,750.

On September 12, 2017, we received a 9-month non-convertible loan of $225,000 from a privately-held investment firm. The loan earns an
annual interest rate of 10%. The Company paid total fees of $25,000 including original issue discount and other costs related to this loan. We
agreed  to  issue  3,333  shares  at  closing.  We  recorded  the  fair  value  of  the  shares  as  a  debt  discount  that  will  be  amortized  to  interest  expense
during the term of the loan. We amortized $15,311 of debt discounts in the year ended December 31, 2017. The unamortized debt discount as of
December  31,  2017  was  $22,689.  In  the  event  of  default  and  at  the  option  of  the  holder,  the  loan  is  convertible  into  common  stock  at  a  35%
discount to the average of the two lowest daily volume weighted average closing stock price for the 20 trading days prior to conversion.

- 73 -

 
 
 
 
 
 
 
Merchant Agreements

We  have  signed  various  Merchant  Agreements  which  entitle  the  lenders  to  our  customer  receipts.  We  accounted  for  the  Merchant
Agreements as loans under ASC 860 because while we provided rights to current and future receipts, we still had control over the receipts. The
following table shows our Merchant Agreements as of December 31, 2017.

Inception Date
January 6, 2016
February 8, 2016
August 16, 2016
August 26, 2016
February 6, 2017
March 2, 2017
June 6, 2017
June 21, 2017
July 17, 2017
September 29, 2017
October 25, 2017
December 7, 2017
December 12, 2017

Purchase
Price

Purchased
Amount

Outstanding
Balance

250,000   
100,000   
70,000   
125,000   
125,000   
75,750   
250,000   
150,000   
125,000   
75,000   
110,000   
160,000   
160,000   
1,775,750    $

322,500    $
129,900   
90,930   
166,250   
161,250   
97,718   
330,000   
190,500   
160,000   
102,000   
153,890   
212,800   
212,800   
2,330,538    $

-   
-   
-   
-   
-   
-   
-   
-   
-   
(1,200)  
71,233   
157,088   
159,186   
386,307   

Daily
Payment    
  1,279.76   
927.00   
650.00   
  1,386.00   
  1,343.75   
775.74   
  1,833.00   
  1,361.00   
  1,250.00   
  1,200.00   
  1,539.00   
  1,251.76   
  1,251.76   

  $

Interest
Rate  

Deferred
Finance
Fees

14% 
15% 
15% 
6% 
15% 
7% 
5% 
15% 
7% 
15% 
15% 
25% 
15% 

2,500   
2,000   
1,590   
2,535   
1,250   
750   
6,250   
1,498   
1,250   
1,500   
8,800   
5,799   
5,258   
  $ 40,980   

Note  
1 

1) The Company recognized a gain in fiscal year 2016 on the settlement of the previous loan of $5,044 which was credited to interest expense.

We  amortized  $312,870  and  $40,802  of  debt  discounts  during  the  years  ended  December  31,  2017  and  2016,  respectively  for  all  non-

convertible notes. The total unamortized discount for all non-convertible notes as of December 31, 2017 was $48,194.

Related Party Notes

During  the  year  ended  December  31,  2016,  the  Company  received  advances  from  certain  officers  of  the  Company  amounting  to  $20,000.

These advances were non-interest bearing and payable on demand. As of December 31, 2016 there are no outstanding notes to related parties.

- 74 -

 
 
 
 
 
   
   
   
 
   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
  
 
 
    
 
  
 
  
 
 
 
 
 
(10) Stockholders’ (Deficit)

Preferred Stock

We are authorized to issue 1,000,000 shares of preferred stock with a par value of $0.01. Of the 1,000,000 shares of preferred stock:

1) 20,000 shares have been designated as Series A Junior Participating Preferred Stock (“Junior A”)

2) 313,960 shares have been designated as Series A Convertible Preferred Stock (“Series A”)

3) 279,256 shares have been designated as Series B Convertible Preferred Stock (“Series B”)

4) 88,098 shares have been designated as Series C Convertible Preferred Stock (“Series C”)

5) 850 shares have been designated as Series D Convertible Preferred Stock (“Series D”)

6) 500 shares have been designated as Series E Convertible Preferred Stock (“Series E”)

7) 240,000 shares have been designated as Series G Convertible Preferred Stock (“Series G”)

8) 10,000 shares have been designated as Series H Convertible Preferred Stock (“Series H”)

9) 21 shares have been designated as Series H2 Convertible Preferred Stock (“Series H2”)

10) 6,250 shares have been designated as Series J Convertible Preferred Stock (“Series J”)

11) 15,000 shares have been designated as Series K Convertible Preferred Stock (“Series K”)

As of December 31, 2017 and 2016, there were no shares of Junior A, and Series A, B, C, and E issued and outstanding.

Series D Convertible Preferred Stock

On November 11, 2011, we completed a registered direct offering, pursuant to which we sold an aggregate of 843 units for a purchase price
of $1,000 per unit, resulting in gross proceeds to us of $843,000 (the “Series D Placement”). Each unit (“Series D Unit”) consisted of (i) one share
of Series D Convertible Preferred Stock, $0.01 par value per share (the “Series D Convertible Preferred Stock”) convertible into 84 shares of our
common  stock,  (subject  to  adjustment  for  stock  splits,  stock  dividends,  recapitalization,  etc.)  and  (ii)  one  five-year  warrant  to  purchase
approximately 21 shares of our common stock at a per share exercise price of $24.30, subject to adjustment as provided in the Warrants (“Series D
Warrant”). The Series D Warrants will be exercisable beginning on May 11, 2012 and until the close of business on the fifth anniversary of the
initial exercise date.

The proceeds from the sale of each Series D Unit were allocated between the Series D Convertible Preferred Stock and the Series D Warrants
based  on  the  residual  method.  The  estimated  fair  value  of  the  Series  D  Warrants  was  determined  using  a  binomial  formula,  resulting  in  an
allocation  of  the  gross  proceeds  of  $283,725  to  the  total  warrants  issued.  The  allocation  of  the  gross  proceeds  to  the  Series  D  Convertible
Preferred Stock was $559,275. In accordance with the provisions of ASC 470-20, an additional adjustment between Additional Paid in Capital
and Accumulated Deficit of $530,140 was recorded to reflect an implicit non-cash dividend related to the allocation of proceeds between the stock
and warrants issued. The $530,140 represents the value of the adjustment to additional paid in capital related to the beneficial conversion feature
of the Series D Convertible Preferred Stock. The value adjustment was calculated by subtracting the fair market value of the underlying common
stock on November 10, 2011 issuable upon conversion of the Series D Convertible Preferred Stock from the fair market value of the Series D
Convertible  Preferred  Stock  as  determined  when  the  Company  performed  a  fair  market  value  allocation  of  the  proceeds  to  the  Series  D
Convertible Preferred Stock and warrants. The warrants are recorded as a liability. See “Warrant Derivative Liability” below.

The Series D Convertible Preferred Stock will rank senior to the Company’s common stock and Series C Convertible Preferred Stock with
respect to payments made upon liquidation, winding up or dissolution. Upon any liquidation, dissolution or winding up of the Company, after
payment of the Company’s debts and liabilities, and before any payment is made to the holders of any junior securities, the holders of Series D
Convertible Preferred Stock will first be entitled to be paid $1,000 per share subject to adjustment for accrued but unpaid dividends.

We may not pay any dividends on shares of common stock unless we also pay dividends on the Series D Convertible Preferred Stock in the
same form and amount, on an as-if-converted basis, as  dividends  actually  paid  on  shares  of  our  common  stock.  Except  for  such  dividends,  no
other dividends may be paid on the Series D Convertible Preferred Stock.

Each share of Series D Convertible Preferred Stock is convertible into 84 shares of common stock (based upon an initial conversion price of
$19.50  per  share)  at  any  time  at  the  option  of  the  holder,  subject  to  adjustment  for  stock  splits,  stock  dividends,  combinations,  and  similar
recapitalization transactions (the “Series D Conversion Ratio”). Subject to certain exceptions, if the Company issues any shares of common stock
or  common  stock  equivalents  at  a  per  share  price  that  is  lower  than  the  conversion  price  of  the  Series  D  Convertible  Preferred  Stock,  the
conversion price will be reduced to the per share price at which such shares of common stock or common stock equivalents are issued. Each share
of Series D Convertible Preferred Stock will automatically be converted into shares of common stock at the Series D Conversion Ratio then in
effect if, after six months from the closing of the Series D Placement, the common stock trades on the OTCQB (or other primary trading market
or exchange on which the common stock is then traded) at a price equal to at least 300% of the then effective Series D Convertible Preferred
Stock conversion price for 20 out of 30 consecutive trading days with each trading day having a volume of at least $50,000. Unless waived under
certain circumstances by the holder of the Series D Convertible Preferred Stock, such holder’s Series D Convertible Preferred Stock may not be
converted if upon such conversion the holder’s beneficial ownership would exceed certain thresholds.

- 75 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
In addition, in the event we consummate a merger or consolidation with or into another person or other reorganization event in which our
shares of common stock are converted or exchanged for securities, cash or other property, or we sell, lease, license or otherwise dispose of all or
substantially  all  of  our  assets  or  we  or  another  person  acquire  50%  or  more  of  our  outstanding  shares  of  common  stock,  then  following  such
event, the holders of the Series D Convertible Preferred Stock will be entitled to receive upon conversion of the Series D Convertible Preferred
Stock  the  same  kind  and  amount  of  securities,  cash  or  property  which  the  holders  of  the  Series  D  Convertible  Preferred  Stock  would  have
received had they converted the Series D Convertible Preferred Stock immediately prior to such fundamental transaction.

The holders of Series D Convertible Preferred Stock are not entitled to vote on any matters presented to the stockholders of the Company for
their action or consideration at any meeting of stockholders of the Company (or by written consent of stockholders in lieu of meeting), except that
the holders of Series D Convertible Preferred Stock may vote separately as a class on any matters that would (i) amend, our Restated Articles of
Organization, as amended, in a manner that adversely affects the rights of the Series D Convertible Preferred Stock, (ii) alter or change adversely
the powers, preferences or rights of the Series D Convertible Preferred Stock or alter or amend the certificate of designation, (iii) authorize or
create any class of shares ranking as to dividends, redemption or distribution of assets upon liquidation senior to, or otherwise pari passu with, the
Series D Convertible Preferred Stock, or (iv) increase the number of authorized shares of Series D Convertible Preferred Stock.

If,  within  12  months  of  the  initial  issuance  of  the  Series  D  Convertible  Preferred  Stock,  we  issue  any  common  stock,  common  stock
equivalents, indebtedness or any combination thereof (a “Subsequent Financing”), the holders of Series D Convertible Preferred Stock will have
the right to participate on a pro-rata basis in up to 50% of such Subsequent Financing.

Series D Warrants

The Series D Warrants originally had an exercise price equal to $24.30 per share of common stock. In April 2012, the number of Series D
Warrants increased by 17,681 to a total of 34,930 and each Series D Warrant had an exercise price reset to $12.00 per share of common stock. In
December of 2013 the number of Series D Warrants increased by 20,958 to a total of 55,887 and each Series D Warrant had an exercise price reset
to $7.50 per share of common stock. The Series D Warrants will be exercisable beginning on the six-month anniversary of the date of issuance
and  expire  five  years  from  the  initial  exercise  date.  The  Series  D  Warrants  permit  the  holder  to  conduct  a  “cashless  exercise”  at  any  time  a
registration statement registering, or the prospectus contained therein, is not available for the issuance of the shares of common stock issuable
upon  exercise  of  the  Series  D  Warrant,  and  under  certain  circumstances  at  the  expiration  of  the  Series  D  Warrants.  The  exercise  price  and/or
number of shares of common stock issuable upon exercise of the Series D Warrants are subject to adjustment for certain stock dividends, stock
splits or similar capital reorganizations, as set forth in the Warrants. The exercise price is also subject to adjustment in the event that we issue any
shares of common stock or common stock equivalents at a per share price that is lower than the exercise price for the Series D Warrants then in
effect. Upon any such issuance, subject to certain exceptions, the exercise price will be reduced to the per share price at which such shares of
common stock or common stock equivalents are issued and number of Series D Warrant shares issuable thereunder shall be increased such that
the  aggregate  exercise  price  payable  thereunder,  after  taking  into  account  the  decrease  in  the  exercise  price,  shall  be  equal  to  the  aggregate
exercise  price  prior  to  such  adjustment.  Unless  waived  under  certain  circumstance  by  the  holder  of  a  Series  D  Warrant,  such  holder  may  not
exercise  the  Series  D  Warrant  if  upon  such  exercise  the  holder’s  beneficial  ownership  of  the  Company’s  common  stock  would  exceed  certain
thresholds.

In  the  event  we  consummate  a  merger  or  consolidation  with  or  into  another  person  or  other  reorganization  event  in  which  our  shares  of
common  stock  are  converted  or  exchanged  for  securities,  cash  or  other  property,  or  we  sell,  lease,  license  or  otherwise  dispose  of  all  or
substantially  all  of  our  assets  or  we  or  another  person  acquire  50%  or  more  of  our  outstanding  shares  of  common  stock,  then  following  such
event,  the  holders  of  the  Series  D  Warrants  will  be  entitled  to  receive  upon  exercise  of  the  Series  D  Warrants  the  same  kind  and  amount  of
securities,  cash  or  property  which  the  holders  would  have  received  had  they  exercised  the  Series  D  Warrants  immediately  prior  to  such
fundamental transaction.

On May 10, 2017, we received net proceeds of $140,214 from the exercise of 19,889 stock purchase warrants from the Series D registered
direct offering on November 10, 2011. In consideration for the warrant exercises, we issued to the investors warrants to purchase 39,778 shares of
our Common Stock at an exercise price per share equal to $8.40 per share. The warrants expire on the third year anniversary date. We determined
the fair value of $186,802 for these warrants and recorded the value as other expenses.

- 76 -

 
 
 
 
 
 
 
 
 
Series G Convertible Preferred Stock

On July 6 and November 15, 2012, we completed a private placement, pursuant to which we sold an aggregate of 4,844 units for a purchase
price of $150.00 per unit (the “Series G Purchase Price”), resulting in gross proceeds to us of $726,600 (the “Series G Private Placement”). Each
unit (“Series G Unit”) consists of (i) one share of Series G Convertible Preferred Stock, $0.01 par value per share (the “Series G Preferred Stock”)
convertible into 1 share of our common stock, (subject to adjustment for stock splits, stock dividends, recapitalization, etc.) and (ii) a three-year
warrant to purchase 1 share of our common stock at a per share exercise price of $15.00 (the “Series G Warrant”). The Series G Warrants will be
exercisable until the close of business on the third anniversary of the applicable closing date of the Series G Private Placement.

Each share of Series G Preferred Stock will receive a cumulative dividend at the annual rate of (i) four percent (4%) on those shares of Series
G Preferred Stock purchased from the Company by an individual purchaser with an aggregate investment of less than $100,000, (ii) six percent
(6%) on those shares of Series G Preferred Stock purchased from the Company by an individual purchaser with an aggregate investment of at
least $100,000 but less than $250,000, and (iii) twelve percent (12%) on those shares of Series G Preferred Stock purchased from the Company by
an individual purchaser with an aggregate investment of at least $250,000. Dividends accruing on the Series G Preferred Stock shall accrue from
day  to  day  until,  and  shall  be  paid  within  fifteen  (15)  days  of,  the  first  anniversary  of,  the  original  issue  date  of  the  Series  G  Preferred  Stock;
provided, however, if any shares of the Company’s Series E Preferred Stock are outstanding at such time, payment of the accrued dividends on
the Series G Preferred Stock shall be deferred until no such shares of Series E Convertible Preferred Stock remain outstanding. The Company
may pay accrued dividends on the Series G Preferred Stock in cash or in shares of its common stock equal to the volume weighted average price
of the common stock as reported by the OTCQB for the ten (10) trading days immediately preceding the Series G’s first anniversary.

At the election of the Company and upon required advanced notice, each share of Series G Preferred Stock will automatically be converted
into shares of common stock at the Conversion Ratio then in effect: (i) if, after 6 months from the original issuance date of the Series G Preferred
Stock, the common stock trades on the OTCQB (or other primary trading market or exchange on which the common stock is then traded) at a
price equal to at least $22.50, for 7 out of 10 consecutive trading days with average daily trading volume of at least 334 shares, (ii) on or after the
first  anniversary  of  the  original  issuance  date  of  the  Series  G  Preferred  Stock  or  (iii)  upon  completion  of  a  firm-commitment  underwritten
registered public offering by the Company at a per share price equal to at least $22.50, with aggregate gross proceeds to the Company of not less
than  $2.5  million.  Unless  waived  under  certain  circumstances  by  the  holder  of  the  Series  G  Preferred  Stock,  such  holder’s  Series  G  Preferred
Stock may not be converted if upon such conversion the holder’s beneficial ownership would exceed certain thresholds.

The holders of Series G Preferred Stock are not entitled to vote on any matters presented to the stockholders of the Company for their action
or consideration at any meeting of stockholders of the Company (or by written consent of stockholders in lieu of meeting), except as required by
law.

Series G Warrants

The Series G Warrants issued in the Series G Private Placement had an exercise price equal to $15.00 per share and expired on July 6, 2015.

- 77 -

 
 
 
 
 
 
 
 
 
Series H Convertible Preferred Stock

On  December  28,  2012  the  Company  amended  the Articles  of  Incorporation  to  authorize  10,000  shares  of  Series  H  Convertible  Preferred
Stock. On January 4, 2013, the Company reported that it had entered into a securities purchase and exchange agreement with an investor, pursuant
to which the Company agreed to exchange 33,334 shares of the Company’s common stock, par value $0.01 per share of common stock held by
the investor for an aggregate of 10,000 shares of a newly created series of preferred stock, designated Series H Convertible Preferred Stock, par
value $0.01 per share (the “Series H Preferred Stock”) in a non-cash transaction. The investor originally purchased the common stock from the
Company for $24.08 per share. The exchange ratio was 4 shares of common stock per share of Series H Preferred Stock at a stated conversion
price of $24.08 per share.

Series H2 Convertible Preferred Stock

On December 23, 2014 the Company amended the Articles of Incorporation to authorize 21 shares of Series H2 Convertible Preferred Stock.
On December 23, 2014, the Company reported that it had entered into a securities purchase and exchange agreement with an investor, pursuant to
which the Company agreed to exchange 70,000 shares of the Company’s common stock, par value $0.01 per share of common stock held by the
investor for an aggregate of 21 shares of a newly created series of preferred stock, designated Series H2 Convertible Preferred Stock, par value
$0.01  per  share  (the  “Series  H2  Preferred  Stock”)  in  a  non-cash  transaction.  The  investor  originally  acquired  the  common  stock  from  the
Company for $7.50 per share in the warrant reset transaction on December 23, 2014. The exchange ratio was 3,334 shares of common stock per
share of Series H2 Preferred Stock at a stated conversion price of $7.50 per share.

Series J Convertible Preferred Stock

On February 6, March 28 and May 20, 2013, the Company entered into a Securities Purchase with various individuals pursuant to which the
Company sold an aggregate of 5,087.5 units for a purchase price of $400.00 per unit (the “Purchase Price”), or an aggregate Purchase Price of
$2,034,700. Each unit purchased in the initial tranche consists of (i) one share of a newly created series of preferred stock, designated Series J
Convertible Preferred Stock, par value $0.01 per share (the “Series J Convertible Preferred Stock”), convertible into 34 shares of the Company’s
common stock, par value $0.01 per share and (ii) a warrant to purchase 34 shares of common stock at an exercise price equal to $12.00 per share.
The warrants expire three years from the issuance date.

From the date of issuance of any shares of Series J Convertible Preferred Stock and until the earlier of the first anniversary of such date, the
voluntary conversion of any shares of Series J Convertible Preferred Stock, or the date of any mandatory conversion (solely under the Company’s
control  based  upon  certain  triggering  events)  of  the  Series  J  Convertible  Preferred  Stock,  dividends  will  accrue  on  each  share  of  Series  J
Convertible  Preferred  Stock  at  an  annual  rate  of  (i)  four  percent  (4%)  of  the  Purchase  Price  on  those  shares  of  Series  J  Convertible  Preferred
Stock purchased from the Company pursuant to the Securities Purchase Agreement by an individual purchaser who purchased from the Company
shares of Series J Convertible Preferred Stock with an aggregate Purchase Price of less than $250,000, and (ii) six percent (6%) of the Purchase
Price on those shares of Series J Convertible Preferred Stock purchased from the Company pursuant to the Securities Purchase Agreement by an
individual  purchaser  who  purchased  shares  of  Series  J  Convertible  Preferred  Stock  with  an  aggregate  purchase  price  of  at  least  $250,000.
Dividends accruing on the Series J Convertible Preferred Stock shall accrue from day to day until the earlier of the first anniversary of the date of
issuance of such shares of Series J Convertible Stock, the voluntary conversion of any shares of Series J Convertible Preferred Stock, or the date
of  any  mandatory  conversion  of  the  Series  J  Convertible  Preferred  Stock,  and  shall  be  paid,  as  applicable,  within  fifteen  (15)  days  of  the  first
anniversary of the original issue date of the Series J Convertible Preferred Stock, within five (5) days of the voluntary conversion of shares of the
Series J Convertible Preferred Stock, or within five (5) days of the mandatory conversion of shares of the Series J Convertible Preferred Stock.
The Company may pay accrued dividends on the Series J Convertible Preferred Stock in cash or, in the sole discretion of the Board of Directors
of the Company, in shares of its common stock in accordance with a specified formula.

Each share of Series J Convertible Preferred Stock is convertible into 34 shares of common stock at the option of the holder on or after the
six-month  anniversary  of  the  issuance  of  such  share,  subject  to  adjustment  for  stock  splits,  stock  dividends,  recapitalizations  and  similar
transactions  (the  “Conversion  Ratio”).  Unless  waived  under  certain  circumstances  by  the  holder  of  Series  J  Convertible  Preferred  Stock,  such
holder’s shares of Series J Convertible Preferred Stock may not be converted if upon such conversion the holder’s beneficial ownership would
exceed certain thresholds.

At the election of the Company and upon required advance notice, each share of Series J Convertible Preferred Stock will automatically be
converted into shares of common stock at the Conversion Ratio then in effect: (i) on or after the six-month anniversary of the original issuance
date of the Series J Convertible Preferred Stock, the common stock trades on the OTCQB (or other primary trading market or exchange on which
the common stock is then traded) at a price per share equal to at least $24.00 for 7 out of 10 consecutive trading days with average daily trading
volume of at least 1,667 shares, (ii) on the first anniversary of the original issuance date of the Series J Convertible Preferred Stock or (iii) within
three days of the completion of a firm-commitment underwritten registered public offering by the Company at a per share price equal to at least
$24.00, with aggregate gross proceeds to the Company of not less than $2.5 million. Unless waived under certain circumstances by the holder of
the Series J Convertible Preferred Stock, such holder’s Series J Convertible Preferred Stock may not be converted if upon such conversion the
holder’s beneficial ownership would exceed certain thresholds.

The holders of Series J Convertible Preferred Stock are not entitled to vote on any matters presented to the stockholders of the Company for
their action or consideration at any meeting of stockholders of the Company (or by written consent of stockholders in lieu of meeting), except as
required by law.

Series J Warrants

The Series J Warrants issued in the Series J Private Placement had an exercise price equal to $12.00 per share and expired on February 6,

March 28 and May 20, 2016.

- 78 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
Registration Rights Agreement

In  connection  with  the  Private  Placement,  the  Company  has  agreed  that,  if,  at  any  time  after  February  1,  2014,  the  Company  files  a
Registration Statement relating to an offering of equity securities of the Company (the “Registration Statement”), subject to certain exceptions,
including  a  Registration  Statement  relating  solely  to  an  offering  or  sale  of  securities  having  an  aggregate  public  offering  price  of  less  than
$5,000,000, the Company shall include in the Registration Statement the resale of the shares of common stock underlying the Warrants. Shares of
common stock issued upon conversion of Series J Convertible Preferred Stock or in payment of the dividend on the Series J Convertible Preferred
Stock will not be registered and will not be subject to registration rights. This right is subject to customary conditions and procedures.

Series K Convertible Preferred Stock

On December 12, 2013, the Company entered into a Securities Purchase with various individuals pursuant to which the Company sold an
aggregate of 4,000 units for a purchase price of $250.00 per unit (the “Purchase Price”), for an aggregate Purchase Price of $1,000,000. Each unit
purchased in the initial tranche consists of (i) one share of a newly created series of preferred stock, designated Series K Convertible Preferred
Stock, par value $0.01 per share (the “Series K Convertible Preferred Stock”), convertible into 34 shares of the Company’s common stock, par
value $0.01 per share and (ii) a warrant to purchase 17 shares of common stock at an exercise price equal to $9.38 per share. The warrants expired
three years from the issuance date. Of the $1,000,000 invested in the Private Placement, $572,044 was received in cash and $427,956 was from
the conversion of outstanding indebtedness and interest. The Company incurred $43,334 of fees in conjunction with this private placement. The
purchasers  in  the  initial  tranche  of  the  private  placement  consisted  of  certain  existing  and  new  investors  in  the  Company  as  well  as  all  of  the
members of the Company’s Board of Directors.

From the date of issuance of any shares of Series K Convertible Preferred Stock and until the earlier of the first anniversary of such date, the
voluntary  conversion  of  any  shares  of  Series  K  Convertible  Preferred  Stock,  or  the  date  of  any  mandatory  conversion  (solely  under  the
Company’s  control  based  upon  certain  triggering  events)  of  the  Series  K  Convertible  Preferred  Stock,  dividends  will  accrue  on  each  share  of
Series  K  Convertible  Preferred  Stock  at  an  annual  rate  of  (i)  four  percent  (4%)  of  the  Purchase  Price  on  those  shares  of  Series  K  Convertible
Preferred Stock purchased from the Company pursuant to the Securities Purchase Agreement by an individual purchaser who purchased from the
Company shares of Series K Convertible Preferred Stock with an aggregate Purchase Price of less than $100,000, and (ii) six percent (6%) of the
Purchase  Price  on  those  shares  of  Series  K  Convertible  Preferred  Stock  purchased  from  the  Company  pursuant  to  the  Securities  Purchase
Agreement by an individual purchaser who purchased shares of Series K Convertible Preferred Stock with an aggregate purchase price of at least
$100,000. Dividends accruing on the Series K Convertible Preferred Stock shall accrue from day to day until the earlier of the first anniversary of
the date of issuance of such shares of Series K Convertible Stock, the voluntary conversion of any shares of Series K Convertible Preferred Stock,
or the date of any mandatory conversion of the Series K Convertible Preferred Stock, and shall be paid, as applicable, within fifteen (15) days of
the first anniversary of the original issue date of the Series K Convertible Preferred Stock, within five (5) days of the voluntary conversion of
shares of the Series K Convertible Preferred Stock, or within five (5) days of the mandatory conversion of shares of the  Series  K  Convertible
Preferred Stock. The Company may pay accrued dividends on the Series K Convertible Preferred Stock in cash or, in the sole discretion of the
Board of Directors of the Company, in shares of its common stock in accordance with a specified formula.

Each share of Series K Convertible Preferred Stock is convertible into 34 shares of common stock at the option of the holder on or after the
six-month  anniversary  of  the  issuance  of  such  share,  subject  to  adjustment  for  stock  splits,  stock  dividends,  recapitalizations  and  similar
transactions (the “Conversion Ratio”). Unless waived under certain circumstances by the holder of Series K Convertible Preferred Stock, such
holder’s shares of Series K Convertible Preferred Stock may not be converted if upon such conversion the holder’s beneficial ownership would
exceed certain thresholds.

At the election of the Company and upon required advance notice, each share of Series K Convertible Preferred Stock will automatically be
converted into shares of common stock at the Conversion Ratio then in effect: (i) on or after the six-month anniversary of the original issuance
date of the Series K Convertible Preferred Stock, the common stock trades on the OTCQB (or other primary trading market or exchange on which
the common stock is then traded) at a price per share equal to at least $24.00 for 7 out of 10 consecutive trading days with average daily trading
volume of at least 1,667 shares, (ii) on the first anniversary of the original issuance date of the Series K Convertible Preferred Stock or (iii) within
three days of the completion of a firm-commitment underwritten registered public offering by the Company at a per share price equal to at least
$24.00, with aggregate gross proceeds to the Company of not less than $2.5 million. Unless waived under certain circumstances by the holder of
the Series K Convertible Preferred Stock, such holder’s Series K Convertible Preferred Stock may not be converted if upon such conversion the
holder’s beneficial ownership would exceed certain thresholds.

- 79 -

 
 
 
 
 
 
 
 
 
The proceeds from the sale of each Series K Unit were allocated between the Series K Convertible Preferred Stock and the Series K Warrants
based  on  the  relative  fair  value  method.  The  estimated  fair  value  of  the  Series  K  Warrants  was  determined  using  a  Black-Scholes  formula,
resulting  in  an  allocation  of  the  gross  proceeds  of  $271,422  to  the  total  warrants  issued.  The  allocation  of  the  gross  proceeds  to  the  Series  K
Convertible Preferred Stock was $685,245, net of $43,334 in fees. In accordance with the provisions of ASC 470-20, an additional adjustment in
the aggregate between Additional Paid in Capital and Accumulated Deficit of $1,495,415 was recorded for all tranches of Series K to reflect an
implicit, deemed non-cash dividend related to the allocation of proceeds between the stock and warrants issued. The $1,495,415 represents the
aggregate value of the adjustment to additional paid in capital related to the beneficial conversion feature of the Series K Convertible Preferred
Stock. The value adjustment was calculated by subtracting the fair market value of the underlying common stock on the closing dates issuable
upon  conversion  of  the  Series  K  Convertible  Preferred  Stock  from  the  fair  market  value  of  the  Series  K  Convertible  Preferred  Stock  as
determined when the Company performed a fair market value allocation of the proceeds to the Series K Convertible Preferred Stock and warrants.

On January 29, 2014, the Company entered into a Securities Purchase Agreement with various accredited investors, pursuant to which the
Company sold an aggregate of 4,875 units for a purchase price of $250.00 per unit or an aggregate Purchase Price of $1,218,750. This was the
second  tranche  of  a  $1.5  million  private  placement  previously  disclosed  by  the  Company  in  its  Current  Report  on  Form  8-K  filed  with  the
Securities and Exchange Commission on December 12, 2013, which is incorporated by reference herein. The Purchasers in the second tranche of
the Private Placement consisted of certain existing and new investors in the Company, as well as all of the members of the Company’s board of
directors.

Each  unit  purchased  in  the  second  tranche  consists  of  (i)  one  share  of  Series  K  Convertible  Preferred  Stock,  par  value  $0.01  per  share,
convertible into 34 shares of the Company’s common stock, par value $0.01 per share and (ii) a warrant to purchase 17 shares of common stock at
an exercise price equal to $9.38 per share, with a term that expired on January 29, 2017.

On February 28, 2014, the Company entered into a Securities Purchase Agreement with various accredited investors, pursuant to which the
Company sold an aggregate of 1,854 units for a purchase price of $340.00 per unit or an aggregate Purchase Price of $630,360. This was the third
tranche of a $1.5 million private placement previously disclosed by the Company in its Current Report on Form 8-K filed with the Securities and
Exchange  Commission  on  December  12,  2013,  which  is  incorporated  by  reference  herein.  The  Purchasers  in  the  third  tranche  of  the  Private
Placement consisted of certain existing and new investors in the Company.

Each  unit  purchased  in  the  third  tranche  consists  of  (i)  one  share  of  Series  K  Convertible  Preferred  Stock,  par  value  $0.01  per  share
convertible into 34 shares of the Company’s common stock, par value $0.01 per share and (ii) a warrant to purchase 17 shares of common stock at
an exercise price equal to $12.75 per share, with a term that expired on February 28, 2017.

On  June  30,  2014,  the  Company  entered  into  a  Securities  Purchase Agreement  with  various  accredited  investors,  pursuant  to  which  the
Company sold an aggregate of 734 units for a purchase price of $300.00 per unit or an aggregate Purchase Price of $220,000. This was the fourth
tranche of a $1.5 million private placement previously disclosed by the Company in its Current Report on Form 8-K filed with the Securities and
Exchange  Commission  on  December  12,  2013,  which  is  incorporated  by  reference  herein.  The  Purchasers  in  the  fourth  tranche  of  the  Private
Placement consisted of certain existing and new investors in the Company.

Each  unit  purchased  in  the  fourth  tranche  consists  of  (i)  one  share  of  Series  K  Convertible  Preferred  Stock,  par  value  $0.01  per  share
convertible into 34 shares of the Company’s common stock, par value $0.01 per share and (ii) a warrant to purchase 17 shares of common stock at
an exercise price equal to $11.25 per share, with a term that expired on June 30, 2017.

On November 12, 2014, the Company entered into a Securities Purchase Agreement with various accredited investors, pursuant to which the
Company sold an aggregate of 1,052 units for a purchase price of $250.00 per unit or an aggregate Purchase Price of $263,000. This was the fifth
tranche of a $1.5 million private placement previously disclosed by the Company in its Current Report on Form 8-K filed with the Securities and
Exchange  Commission  on  December  12,  2013,  which  is  incorporated  by  reference  herein.  The  Purchasers  in  the  fourth  tranche  of  the  Private
Placement consisted of certain existing and new investors in the Company.

- 80 -

 
 
 
 
 
 
 
 
 
 
Each  unit  purchased  in  the  fifth  tranche  consists  of  (i)  one  share  of  Series  K  Convertible  Preferred  Stock,  par  value  $0.01  per  share
convertible into 34 shares of the Company’s common stock, par value $0.01 per share and (ii) a warrant to purchase 17 shares of common stock at
an exercise price equal to $9.38 per share, with a term that expired on November 12, 2017.

The  Private  Placement  was  originally  expected  to  raise  $1.5  million  and  close  on  or  before  January  31,  2014.  On  January  29,  2014,  the
Company’s  Board  of  Directors  voted  to  increase  the  subscription  amount  of  the  Private  Placement  by  $718,750.  The  Board  of  Directors  also
voted to extend the Private Placement until February 28, 2014. On February 28, 2014 the Company’s Board of Directors voted to increase the
subscription amount once again to a total of $3.5 million and extended the closing to April 4, 2014. On April 13, 2014 the Company’s Board of
Directors voted to increase the subscription amount by $1 million, to a total of $4.5 million, and extended the closing to May 31, 2014. On July 7,
2014  the  Company’s  Board  of  Directors  voted  to  extend  the  closing  to August  15,  2014.  Together  with  the  initial  tranche  of  $1,000,000  that
closed on December 12, 2013, the second tranche of $1,218,750 that closed January 29, 2014, the third tranche of $630,360 that closed February
28, 2014, the fourth tranche of $220,000 that closed June 30, 2014, and the fifth tranche of $263,000 that closed November 12, 2014,the total
consideration received by the Company in the Private Placement is $3,332,110, which is comprised of $2,511,404 in cash and $820,706 from the
conversion of outstanding indebtedness and Board of Director fees. The placement was closed after the November 12, 2014 round.

On September 22, 2014 the Company issued 2,134 shares of common stock for the conversion of 64 shares of Series K Preferred Convertible
Stock. In 2017, the Company returned 64 shares of Series K Preferred Convertible Stock as issued and outstanding. The conversion was done
erroneously. The Company agreed to allow the recipient to keep the 2,134 shares of common stock, with a fair value of $15,992, as issued for
services to be rendered in 2018 for the Company.

In connection with the Series K Warrants, we calculated the fair value of the warrants received as described above using the Black- Scholes

formula with the below assumptions:

Assumptions
Contractual life (in
months)
Expected volatility    
Risk-free interest
rate
Exercise price
Fair value per
warrant

  $

  $

Series K 
Warrants
December 12, 2013  

Series K 
Warrants

Series K 
Warrants

January 29, 2014  

February 28, 2014  

Series K 
Warrants 
June 30, 2014

Series K 
Warrants
November 12, 2014  

36 
136.1 

0.39%   
  $
9.38 

6.00 

  $

36 
152.4 

0.39%   
  $
9.38 

9.00 

  $

36 
152.7 

0.39%   
  $
12.75 

11.10 

  $

36 
153.9 

0.90%   
  $
11.25 

8.70 

  $

36 
153.9 

0.90%
9.38 

6.90 

The holders of Series K Convertible Preferred Stock are not entitled to vote on any matters presented to the stockholders of the Company for
their action or consideration at any meeting of stockholders of the Company (or by written consent of stockholders in lieu of meeting), except as
required by law.

Series K Warrants

The warrants issued in the Private Placement have an exercise price equal to $9.38 per share, for the December 12, 2013 and January 29, 2014
warrants,  $12.75  per  share  for  the  February  28,  2014  warrants,  $11.25  per  share  for  the  June  30,  2014  warrants  and  $9.38  per  share  for  the
November 12, 2014 warrants, with a term expiring three years from the issuance date. The warrants also permit the holder to conduct a “cashless
exercise” at any time the holder of the warrant is an affiliate of the Company. The exercise price and/or number of shares issuable upon exercise
of  the  warrants  will  be  subject  to  adjustment  for  stock  dividends,  stock  splits  or  similar  capital  reorganizations,  as  set  forth  in  the  warrant
agreement.

Subject to the terms and conditions of the warrants, at any time commencing six months from the closing date of the sale of Units under the
Securities  Purchase Agreement  the  Company  has  the  right  to  call  the  warrants  for  cancellation  if  the  volume  weighted  average  price  of  its
common stock on the OTCQB (or other primary trading market or exchange on which the common stock is then traded) equals or exceeds three
times the per share exercise price of the warrants for either (i) 10 consecutive trading days or (ii) 15 out of 25 consecutive trading days. All Series
K warrants have expired.

- 81 -

 
 
 
 
 
 
 
 
 
 
 
 
   
   
   
   
   
   
   
   
   
   
 
 
 
 
 
Registration Rights Agreement

In  connection  with  the  Private  Placement,  the  Company  has  agreed  that,  if,  at  any  time  after  February  1,  2014,  the  Company  files  a
Registration Statement relating to an offering of equity securities of the Company (the “Registration Statement”), subject to certain exceptions,
including  a  Registration  Statement  relating  solely  to  an  offering  or  sale  of  securities  having  an  aggregate  public  offering  price  of  less  than
$5,000,000, the Company shall include in the Registration Statement the resale of the shares of common stock underlying the warrants. Shares of
common  stock  issued  upon  conversion  of  Series  K  Convertible  Preferred  Stock  or  in  payment  of  the  dividend  on  the  Series  K  Convertible
Preferred Stock will not be registered and will not be subject to registration rights. This right is subject to customary conditions and procedures.

Common Stock

Stock Options and Warrants

Our stockholders approved our amended 2005 Equity Incentive Plan (the “2005 Plan”) pursuant to which an aggregate of 1,800,000 shares of
our common stock were reserved for issuance upon exercise of stock options or other equity awards made under the 2005 Plan. Under the 2005
Plan,  we  may  award  stock  options,  shares  of  common  stock,  and  other  equity  interests  in  the  Company  to  employees,  officers,  directors,
consultants, and advisors, and to any other persons the Board of Directors deems appropriate. As of December 31, 2017, options to acquire 35,274
shares were outstanding under the 2005 Plan.

On  December  12,  2013  at  the  Company’s  special  meeting  the  shareholders  approved  the  2013  Equity  Incentive  Plan  (the  “ 2013  Plan”)
pursuant to which 3,000,000 shares of our common stock were reserved for issuance upon exercise of stock options or other equity awards under
the 2013 Plan. Under the Plan, we may award stock options, shares of common stock, and other equity interests in the Company to employees,
officers,  directors,  consultants,  and  advisors,  and  to  any  other  persons  the  Board  of  Directors  deems  appropriate. As  of  December  31,  2017,
options to acquire 82,481 shares were outstanding under the Plan with 2,917,519 shares available for future grant under the 2013 Plan.

- 82 -

 
 
 
 
 
 
 
 
On November 29, 2015 the Company’s Board of Directors adopted the 2015 Nonqualified Stock Option Plan (the “ 2015 Plan”) pursuant to
which 5,000,000 shares of our common stock were reserved for issuance upon exercise of non-qualified stock options under the 2015 Plan. Under
the Plan, we may award non-qualified stock options in the Company to employees, officers, directors, consultants, and advisors, and to any other
persons the Board of Directors deems appropriate. As of December 31, 2017, non-qualified options to acquire 129,937 shares were outstanding
under the Plan with 4,870,063 shares available for future grants under the 2015 Plan.

All  of  the  outstanding  non-qualified  options  had  an  exercise  price  that  was  at  or  above  the  Company’s  common  stock  share  price  on

December 31, 2017.

The following tables summarize information concerning options and warrants outstanding and exercisable:

Stock Options

Warrants

Total

Balance outstanding, January 1, 2016

Granted
Exercised
Expired
Forfeited

Balance outstanding, December 31, 2016

Granted
Exercised
Expired
Forfeited

Balance outstanding, December 31, 2017

Weighted
Average
price per
share

13.20     
-     
-     
30.00     
15.30     
12.60     
8.40     
-     
30.00     
9.82     
10.95     

Shares

185,708    $
-     
-     
(6,200)    
(3,866)    
175,642    $
87,198     
-     
(3,202)    
(11,946)    
247,692    $

Shares

974,256    $
272,652     
(2,334)    
(362,585)    
-     
881,989    $
245,661     
(19,889)    
(208,219)    
-     
899,542    $

Weighted
Average
price per
share

13.20     
12.60     
9.30     
16.50     
-     
12.00     
11.14     
7.50     
11.46     
-     
12.03     

Shares
1,159,964     
272,652     
(2,334)    
(368,785)    
(3,866)    
1,057,631     
332,859     
(19,889)    
(211,421)    
(11,946)    
1,147,234     

    Exercisable  
1,055,483 

991,032 

1,073,850 

Range of 

Exercise Prices    

Number of
Options

Options Outstanding
Weighted Average
Remaining
Contractual
Life (Years)

Exercise 
Price

Number of
Options

Options Exercisable
Weighted Average
Remaining
Contractual
Life (Years)

Exercise 
Price

$

$

7.50 - $11.99     
12.00 – 14.99     
15.00 – 17.99     
18.00 – 20.99     
21.00 – 30.00     
7.50 - $30.00     

133,580     
88,705     
7,547     
12,854     
5,006     
247,692     

8.3    $
7.7     
4.6     
2.1     
2.7     
7.5    $

8.63     
12.00     
15.00     
18.00     
30.00     
10.95     

80,997     
67,904     
7,547     
12,854     
5,006     
174,308     

7.7    $
7.6     
4.6     
2.1     
2.7     
7.0    $

8.78 
12.00 
15.00 
18.00 
30.00 
11.59 

There was $392,590 of total unrecognized compensation cost, net of estimated forfeitures, related to non-vested stock options granted as of
December 31, 2017. This cost is expected to be recognized over a period of 1.66 years, and will be adjusted for any future changes in estimated
forfeitures.

The Series D Warrants issued in connection with the registered direct offering of Series D Convertible Preferred were measured at fair value
and  liability-classified  because  the  Series  D  Warrants  contain  “down-round  protection”  and  therefore,  do  not  meet  the  scope  exception  for
treatment  as  a  derivative  under  ASC  815, Derivatives  and  Hedging,  (“ASC  815”).  Since  “down-round  protection”  is  not  an  input  into  the
calculation of the fair value of the warrants, the warrants cannot be considered indexed to the Company’s own stock which is a requirement for
the scope exception as outlined under ASC 815. The estimated fair value of the warrants was determined using the binomial model, resulting in
an  allocation  of  the  gross  proceeds  $171,733  to  the  warrants  issued  in  the  Series  D  registered  direct  offering.  The  fair  value  was  affected  by
changes in inputs to that model including our stock price, expected stock price volatility, the contractual term, and the risk-free interest rate. We
early  adopted ASU  2017-11  and  applied  the  guidance  to  derivative  accounting.  Therefore,  we  reclassified  the  warrant  and  conversion  option
liabilities to equity and stopped fair valuing the instruments in 2017.

In  connection  with  the  senior  secured  convertible  debentures  issued  in  our  private  placement  with  closings  in  2015  and  2016,  we  issued
warrants to the lenders to purchase an aggregate 292,262 and 83,902 shares of the Common Stock, respectively, at an exercise price of $12.00 per
share, expiring five years after the issuance date. We also issued, in 2015 and 2016, warrants to the placement agent to purchase an aggregate
56,310 and 13,750 shares of the Common Stock, respectively, at an exercise price of $12.00 per share, expiring five years after the issuance date.

- 83 -

 
 
 
 
 
 
 
   
   
 
 
 
   
   
   
   
   
   
  
   
  
   
  
   
  
   
   
  
   
  
   
  
   
  
   
 
 
   
   
 
 
   
   
 
   
   
   
   
   
 
 
 
 
 
 
 
 
 
In  November  2016  we  issued  warrants  to  purchase  11,000  shares  of  restricted  common  stock  to  an  investor  relations  firm  for  services

rendered with a total fair value of $84,735.

In January 2017, we issued warrants to purchase 3,334 shares of restricted common stock with a fair value of $15,558 to an investor relations

firm for services performed.

Common Stock Issuances

On various dates from January to March 2017, the Company issued 27,000 shares of restricted common stock to investors as compensation

for loans provided to us.

On June 9, 2017, one shareholder converted 6,000 shares of Series G Convertible Preferred Stock into 2,000 shares of common stock and

converted 63 shares of Series J Convertible Preferred Stock into 2,100 shares of common stock.

On various dates for the year ended December 31, 2017, the Company issued 61,307 shares of common stock based on the 10-day VWAP
prior to quarter end to holders of the Debentures in payment of the quarterly interest accrued from the Debentures first anniversary date through
June 30, 2017 for an aggregate amount of $483,054. We recognized a $218,452 gain on extinguishment of debt by calculating the difference of
the shares valued on the issuance date and the amount of accrued interest through June 30, 2017.

On April 1, 2017, we issued 1,667 shares of restricted common stock to an investor relations firm and recorded the common stock’s fair value

of $15,000 as administrative expense in the year ended December 31, 2017.

On April 19, 2017, we received a 7-month non-convertible loan of $250,000 from a privately-held investment firm. The loan earns an annual
interest rate of 10% and includes a 10% original issue discount. We agreed to issue 833 shares at closing. Until the loan was repaid, we agreed
that over the next one hundred eighty (180) days to issue 2,500 shares to the Investor every sixty (60) days for a total issuance of 8,333 shares. As
of December 31, 2017, the outstanding balance on this note was zero. We have issued 8,333 shares including the closing shares since inception of
the loan.

The Revolving Note was amended on May 2, 2017 to increase the aggregate principal amount to $3,000,000. In exchange for this increase,
we agreed to issue 16,667 shares of our Common Stock to the Investor, to decrease the exercise price per share of the warrants to the lower of (i)
$12.00 or (ii) the per share purchase price of the shares of our Common Stock sold in a qualified offering, and to change the trigger date in the
Revolving Note from April 28, 2017 (the six month anniversary of October 28, 2016) to July 25, 2017. The Revolving Note was further amended
on August 18, 2017 to increase the aggregate principal amount to $3,500,000 with all other terms unchanged.

On May 10, 2017, we received $149,164 from the exercise of 19,889 stock purchase warrants from the Series D registered direct offering on
November 10, 2011. We paid $8,950 to a broker in connection with the warrant exercises. In consideration for the warrant exercises, we issued to
the investors warrants to purchase 39,778 shares of our Common Stock at an exercise price per share equal to $8.40 per share. The warrants expire
on the third year anniversary date. We determined the fair value of $186,802 for these warrants and recorded the value as other expenses.

On September 12, 2017, we received a 9-month non-convertible loan of $225,000 from a privately-held investment firm. The loan earns an
annual interest rate of 10%. The Company paid total fees of $25,000 including original issue discount and other costs related to this loan. We
agreed to issue 3,333 shares at closing. We recorded the fair value of the shares amounting to $13,000 as a debt discount that will be amortized to
interest expense during the term of the loan.

On September 20, 2017, we issued 4,000 shares of restricted common stock to an investor relations firm and recorded the common stock’s

fair value of $16,000 as administrative expense in the year ended December 31, 2017.

On December 11, 2017 the Company issued 2,500 shares with a fair value of $9,500 to a noteholder for an extension on a loan of $170,000

until February 15, 2018.

On December 12, 2017, we issued 150,000 shares of restricted common stock in connection with the acquisition of assets of BaroFold Corp.

We recorded the common stock’s fair value of $600,000 as part of Intangibles cost in the consolidated balance sheets.

On December 31, 2017, the Company awarded 2,134 shares of common stock for services to be rendered in 2018 for the Company.

During the quarter ended December 31, 2017, the Company issued 9,700 shares of common stock on its convertible notes. The relative fair

value of these notes totaling $37,435 was recognized as a debt discount to the notes (see Note 9).

- 84 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
(11) Subsequent Events

During  the  three  months  ended  March  31,  2018,  we  issued  to  Debenture  holders  12,494  shares  of  common  stock  for  quarterly  interest  of
$52,371 issued in stock in lieu of cash. Of the 12,494 shares issued, 1,092 shares were issued to members of the Company’s Board of Directors,
who are also Debenture holders.

On various dates in March 2018, we received six-month, convertible loans of $150,000 from three accredited investors. The loans include a

10% original issue discount and are convertible into a qualified offering at the deal price.

On March 14, 2018, we received a three month, non-convertible loan of $50,000 from a related party who was a member of the Company’s

Board of Directors.

On  March  12,  2018,  we  received  a  six-month,  convertible  loan  of  $253,000  from  an  accredited  investor.  The  loan  has  an  original  issue
discount of $53,000. The loan can be converted at any time into common stock at a conversion price of $7.50. We agreed to issue the investor
6,750 shares of restricted common stock.

On March 12, 2018, we received a six-month convertible loan of $85,000 from a privately-held investment firm. The Company paid total

fees of $5,400 related to this loan.

The Revolving Note was further amended on January 30, 2018 to increase the aggregate principal amount to $4,000,000 with all other terms

unchanged. We received the additional $500,000 advances in January and March 2018.

On February 22, 2018 we signed a Merchant Agreement with a lender. Under the agreement we received $110,000 in exchange for rights to
all customer receipts until the lender is paid $147,400, which is collected at the rate of $921.25 per business day. The payments were secured by
second position rights to all customer receipts until the loan has been paid in full.

On February 12, 2018, we received a six-month, convertible loan of $100,000 from an accredited investor. The loan earns a one-time interest
of 10%. $50,000 of the proceeds were used to pay off the outstanding balance of a previous loan from this lender. The loan can be converted at
any time into common stock at a conversion price of $7.50. We issued the investor 5,000 shares of restricted common stock.

On February 12, 2018, we issued 3,500 shares of restricted common stock to an accredited investor to extend the maturity date of our eight-
month,  non-convertible  loan  of  $170,000  originated  on  March  21,  2017  to  February  15,  2018.  The  accredited  investor  agreed  to  a  further
extension to March 31, 2018 in exchange for 3,500 shares of restricted common stock yet to be issued.

On January 19, 2018, we received a six-month, convertible loan of $150,000 from an accredited investor. The loan earns a one-time interest
of  10%  and  includes  a  10%  original  issue  discount.  We  also  issued  the  investor  4,000  shares  of  restricted  common  stock.  The  loan  can  be
converted at any time into common stock at a conversion price of $7.50.

On January 16, 2018, we received a one year, convertible loan of $131,250 from an accredited investor. The loan earns interest of 4% per

year. The loan can be converted into common stock after 180 calendar days at a discount of 40% to market price as defined in the loan.

On January 3, 2018, we received a one year, convertible loan of $95,000 from an accredited investor. The loan includes a 5% original issue

discount. The loan can be converted into common stock after 180 calendar days at a discount of 40% to market price as defined in the loan.

- 85 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
ITEM 9. CHANGES IN AND DISAGREEMENTS WITH ACCOUNTANTS ON ACCOUNTING AND FINANCIAL DISCLOSURE.

None

ITEM 9A. CONTROLS AND PROCEDURES

Evaluation of Disclosure Controls and Procedures

We  maintain  disclosure  controls  and  procedures  that  are  designed  to  ensure  that  information  required  to  be  disclosed  in  our  Securities
Exchange Act of 1934 filings are recorded, processed, summarized and reported within the time periods specified in the SEC’s rules and forms,
and that such information is accumulated and communicated to our management, including our President and Chief Executive Officer (Principal
Executive  Officer)  and  Chief  Financial  Officer  (Principal  Financial  Officer),  as  appropriate,  to  allow  timely  decisions  regarding  required
disclosure.  In  designing  and  evaluating  the  disclosure  controls  and  procedures,  management  recognized  that  any  controls  and  procedures,  no
matter how well designed and operated, can provide only reasonable assurance of achieving the desired control objectives, as ours are designed
to  do,  and  management  was  necessarily  required  to  apply  its  judgment  in  evaluating  the  cost-benefit  relationship  of  possible  controls  and
procedures.

As of December 31, 2017, we carried out an evaluation, under the supervision and with the participation of our management, including our
principal  executive  officer  and  principal  financial  officer,  of  the  effectiveness  of  the  design  and  operation  of  our  disclosure  controls  and
procedures, as defined in Rules 13a-15(e) and 15d-15(e) under the Securities Exchange Act of 1934. Based upon that evaluation, our principal
executive officer and principal financial officer concluded that our disclosure controls and procedures were not effective as of December 31, 2017
due to limited resources for adequate personnel to prepare and file reports under the Securities Exchange Act of 1934 within the required periods,
and material weaknesses in our internal control over financial reporting relating to our accounting for complex equity transactions as described
below under the heading “Report of Management on Internal Control over Financial Reporting”. Management plans to remediate this weakness
by taking the actions described below.

Report of Management on Internal Control over Financial Reporting

We  are  responsible  for  establishing  and  maintaining  adequate  internal  control  over  financial  reporting.  Internal  control  over  financial
reporting is defined in Rule 13a-15(f) and 15d-15(f) under the Exchange Act, as a process designed by, or under the supervision of our principal
executive and principal financial officers and effected by our board of directors, management and other personnel to provide reasonable assurance
regarding  the  reliability  of  financial  reporting  and  the  preparation  of  financial  statements  for  external  purposes  in  accordance  with  generally
accepted accounting principles and includes those policies and procedures that:

● pertain to the maintenance of records that in reasonable detail accurately and fairly reflect the transactions and disposition of our assets;

● provide reasonable  assurance  that  transactions  are  recorded  as  necessary  to  permit  preparation  of  financial  statements  in  accordance with
generally accepted accounting principles, and that our receipts and expenditures are being made only in accordance with authorization of our
management and directors; and

● provide reasonable assurance regarding prevention or timely detection of unauthorized acquisition, use or disposition of our assets that could

have a material effect on the financial statements.

Our internal control system is designed to provide reasonable assurance to our management and board of directors regarding the preparation
and fair presentation of financial statements. Because of its inherent limitations, internal control over financial reporting may not prevent or detect
misstatements.  Projections  of  any  evaluation  of  effectiveness  to  future  periods  are  subject  to  the  risks  that  controls  may  become  inadequate
because of changes in conditions, or that the degree of compliance with the policies or procedures may deteriorate.

We have assessed the effectiveness of our internal control over financial reporting as of December 31, 2017. In making this assessment, we
used the criteria set forth by the Committee of Sponsoring Organizations of the Treadway Commission (COSO) in Internal Control-Integrated
Framework (2013).

Based on this assessment, management believes that, as of December 31, 2017, the Company did not maintain effective internal control over

financial reporting because of the effect of material weaknesses in our internal control over financial reporting discussed below.

Public  Company  Accounting  Oversight  Board  Auditing  Standard  No.  2  defines  a  material  weakness  as  a  significant  deficiency,  or
combination  of  significant  deficiencies,  that  results  in  there  being  a  more  than  remote  likelihood  that  a  material  misstatement  of  the  annual  or
interim financial statements will not be prevented or detected on a timely basis. Based upon this definition, our management concluded that, as of
December  31,  2017,  a  material  weakness  existed  in  our  internal  control  over  financial  reporting  related  to  accounting  for  complex  equity
transactions.

- 86 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Specifically, we identified material weaknesses in our internal control over financial reporting related to the following matters:

● We identified a lack of sufficient segregation of duties. Specifically, this material weakness is such that the design over these  areas relies

primarily on detective controls and could be strengthened by adding preventative controls to properly safeguard Company assets.

● Management has identified a lack of sufficient personnel in the accounting function due to our limited resources with appropriate skills,
training and experience to perform the review processes to ensure the complete and proper application of generally accepted accounting
principles,  particularly  as  it  relates  to  valuation  of  warrants  and  other  complex  debt  /equity  transactions.  Specifically, this  material
weakness resulted in audit adjustments to the annual consolidated financial statements and revisions to related disclosures.

● Limited policies and procedures that cover recording and reporting of financial transactions.

● Lack of multiple levels of review over the financial reporting process

Our plan to remediate those material weaknesses is as follows:

● Improve the effectiveness of the accounting group by augmenting our existing resources with additional consultants or employees to assist
in  the  analysis  and  recording  of  complex  accounting  transactions,  and  to  simultaneously  achieve  desired  organizational structuring  for
improved  segregation  of  duties.  We  plan  to  mitigate  this  identified  deficiency  by  hiring  an  independent  consultant  once  we  generate
significantly more revenue or raise significant additional working capital.

● Improve expert  review  and  achieve  desired  segregation  procedures  by  strengthening  cross  approval  of  various  functions  including

quarterly internal audit procedures where appropriate.

Changes in Internal Control Over Financial Reporting

There  have  been  no  changes  in  our  internal  control  over  financial  reporting  that  occurred  during  the  fourth  quarter  of  2017  that  have

materially affected, or are reasonably likely to materially affect, our internal control over financial reporting.

ITEM 9B. OTHER INFORMATION.

None.

- 87 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
PART III

ITEM 10. DIRECTORS, EXECUTIVE OFFICERS AND CORPORATE GOVERNANCE

Directors

The following table sets forth information about the individuals who serve as our directors as of December 31, 2017.

Name

Age

Position

Board Committees

Richard T. Schumacher

67

  President, Chief Executive Officer,

Treasurer, Clerk and Director

Jeffrey N. Peterson

Dr. Mickey Urdea

Vito J. Mangiardi

Kevin A. Pollack

62

65

69

47

  Chairman of the Board

  Audit, Compensation, Nominating

  Director

  Director

  Director

  Scientific Advisory Board

  Audit, Compensation, Nominating

  Audit, Compensation, Nominating

Term of
office
expires:

2020

2018

2018

2019

2019

The following noteworthy experience, qualifications, attributes and skills for each Board member, together with the biographical information for
each nominee described below, led to our conclusion that the person should serve as a director in light of our business and structure:

Mr. Richard T. Schumacher, the founder of the Company, has served as a director of the Company since 1978. He has served as the Company’s
Chief  Executive  Officer  since April  16,  2004  and  President  since  September  14,  2004.  He  previously  served  as  Chief  Executive  Officer  and
Chairman of the Board of the Company from 1992 to February 2003. From July 9, 2003 until April 14, 2004 he served as a consultant to the
Company pursuant to a consulting agreement. He served as President of the Company from August 1978 to August 1999. Mr. Schumacher served
as the Director of Infectious Disease Services for Clinical Sciences Laboratory, a New England-based medical reference laboratory, from 1986 to
1988. From 1972 to 1985, Mr. Schumacher was a research scientist and clinical laboratory director at the Center for Blood Research, a nonprofit
medical  research  institute  associated  with  Harvard  Medical  School.  Mr.  Schumacher  received  a  B.S.  in  Zoology  from  the  University  of  New
Hampshire.

- 88 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Mr. Jeffrey N. Peterson  has served as a director of the Company since July 2011 and as Chairman of the Board starting in 2012. Since 1999, he
has served as the Chief Executive Officer of Target Discovery, Inc. (“TDI”), a personalized medicine diagnostics (PMDx) company. Mr. Peterson
also serves as Chairman and CEO of TDI’s majority-owned subsidiary, Veritomyx, Inc., which is completing development and commercialization
of software tools for accurate peptide, protein and isoform identification and characterization. Prior to incorporating and joining TDI, Mr. Peterson
served  as  CEO  of  Sharpe,  Peterson,  Ocheltree  & Associates,  an  international  business  development  consulting  firm  assisting  Fortune  500  and
many  smaller  firms  in  business  expansion  and  strategy.  Prior  to  that,  he  spent  9  years  in  key  management  roles  in  Abbott  Laboratories’
Diagnostics  and  International  (Pharmaceuticals,  Hospital  Products,  Nutritionals,  and  Consumer)  businesses,  last  serving  as  CEO  and  General
Manager of Abbott South Africa. Mr. Peterson’s experience prior to Abbott Laboratories included 11 years with General Electric’s Engineered
Materials  and  Plastics  businesses,  spanning  roles  in  strategic  planning,  business  development,  technology  licensing,  marketing  and  sales,
operations, quality control and R&D. Mr. Peterson holds BSChE and MSChE (Chemical Engineering) degrees from MIT, as well as 6 issued US
patents. He served as Chair Emeritus of the BayBio Institute, a non-profit organization serving the life science community, and on the Board of
BayBio,  a  trade  association  for  the  life  sciences  industry  in  Northern  California.  He  served  as  a  cofounder  of  the  Coalition  for  21st  Century
Medicine,  and  of  BIO’s  Personalized  Medicine  &  Diagnostics  Working  Group.  He  served  on  the  Board  of  Advisors  for  the  Center  for
Professional Development and Entrepreneurship at the University of Texas MD Anderson Cancer Center. He currently serves on the Advisory
Board of the California Technology Council.

Mr. Vito J. Mangiardi  has served as a director of the Company since July 2012. Mr. Mangiardi is an accomplished senior executive with proven
experience as a President, CEO and COO in the Life Sciences and Bio-Energy product and service sectors. He is a strong P&L performer and
corporate strategist in General Management, Operations, Sales/Marketing, and Science. Mr. Mangiardi has held positions as a Research Chemist
for Bio-Rad Laboratories, Inc.; Sales & Marketing Director for Baxter Travenol, Inc.; Executive VP and COO for Quintiles Transnational Corp.;
President and CEO of Diagnostics Laboratories, Inc., Clingenix, Inc., and Bilcare, Inc.; and President of AAI Pharma, Inc. More recently he was
the COO/Deputy Director of Operations and Production at the University of California Lawrence  Berkeley  National  Laboratory  Joint  Genome
Institute. Mr. Mangiardi has experience with three start-ups, two midsize, and several mature companies, and has international experience leading
and  managing  organizations  on  four  continents.  He  has  vast  experience  in  leading  alliances,  acquisitions,  due  diligence,  and  post-acquisition
assimilation. Mr. Mangiardi has been on the Board of Directors of three companies and has proven success in working with both national and
international investment groups to raise funds. Mr. Mangiardi earned a BS in Biology/Chemistry from Eastern Illinois University and two MBA
degrees  from  Golden  Gate  University  -  in  General  Management  and  in  Marketing.  Mr.  Mangiardi  is  listed  as  an  inventor  in  four  patents  and
various  publications  in  protein  separation  techniques  in  the  area  of  metabolism,  thyroid,  anemia/hematology  and  cancer,  and  is  a  member  of
numerous professional organizations. Mr. Mangiardi is the founding partner, President and CEO of Marin Bay Partners, LLC (MBP), a consulting
firm focused on life sciences, pharmaceutical development and clinical diagnostics.

Mr. Kevin A. Pollack has served as a director of the Company since July 2012. Mr. Pollack serves as an advisor to Opiant Pharmaceuticals, Inc.
(OPNT-NASDAQ),  a  specialty  pharmaceutical  company  developing  pharmacological  treatments  for  substance  use,  addictive,  and  eating
disorders. He previously served as its Chief Financial Officer and as a member of its Board of Directors from 2012 until 2017. He also serves as
President  of  Short  Hills  Capital  LLC,  where  he  provides  a  range  of  services.  Previously,  Mr.  Pollack  worked  in  asset  management  at  Paragon
Capital LP, focusing primarily on U.S.-listed companies, and as an investment banker at Banc of America Securities LLC, focusing on corporate
finance and mergers and acquisitions. Mr. Pollack started his career at Sidley Austin LLP (formerly Brown & Wood LLP) as a securities attorney
focusing  on  corporate  finance,  and  mergers  and  acquisitions.  He  currently  sits  on  the  Board  of  Directors  of  MagneGas  Corporation  (MNGA-
NASDAQ), the developer of a technology that converts liquid waste into a hydrogen-based metal-working fuel and natural gas alternative. Mr.
Pollack graduated magna cum laude from the Wharton School of the University of Pennsylvania and received a dual J.D./M.B.A. from Vanderbilt
University, where he graduated with Beta Gamma Sigma honors.

Dr. Michael S. “Mickey” Urdea has served as a director of the Company since February 8, 2013. Dr. Urdea founded and is a Founder and Partner
for  Halteres  Associates,  a  biotechnology  consulting  firm.  He  also  founded  and  served  as  Chief  Executive  Officer  of  Tethys  Bioscience,  a
proteomics-based diagnostics company involved in preventative personalized medicine. Additionally, Dr. Urdea is a founder and the Chairman of
Catalysis  Foundation  for  Health,  an  organization  addressing  gaps  in  global  healthcare  caused  by  inefficiencies  in  disease  diagnosis  and
monitoring. He serves as an expert consultant to the life sciences industry and is on the scientific advisory boards and boards of directors of a
number of biotechnology, diagnostics, venture capital and philanthropic organizations. Prior to his current business activities, Dr. Urdea founded
the Nucleic Acid Diagnostics group at Chiron Corporation, and with colleagues, invented branched DNA molecules for amplification of signal in
nucleic acid complexes. Application of this technology resulted in the first commercial products for quantification of human hepatitis B, hepatitis
C,  and  human  immunodeficiency  viruses  (HBV,  HCV,  and  HIV,  respectively).  He  then  became  business  head  of  the  Molecular  Diagnostics
Group  and  Chief  Scientific  Officer  at  Bayer  Diagnostics.  He  continues  to  serve  as  a  diagnostics  industry,  product  development  and  scientific
advisor to the Bill and Melinda Gates Foundation, acted as co-chair of two of the Grand Challenges grant review committees, and served as a
member  of  its  Diagnostic  Forum.  Dr.  Urdea  is  an  author  on  nearly  200  peer-reviewed  scientific  publications,  nearly  300  abstracts  and
international scientific presentations, and more than 100 issued and pending patents. He received his BS in Biology and Chemistry from Northern
Arizona University in Flagstaff and his Ph.D in Biochemistry from Washington State University.

- 89 -

 
 
 
 
 
 
Executive Officers

Our  executive  officers  are  appointed  by,  and  serve  at  the  discretion  of,  our  board  of  directors.  The  following  table  sets  forth  information

about our executive officers.

Name
Richard T. Schumacher
Edmund Ting, Ph.D.
Nathan P. Lawrence, Ph.D.
Alexander Lazarev, Ph.D.
Joseph L. Damasio, Jr.

Age
67
64
63
53
43 

Position

  President, Chief Executive Officer, Treasurer, Clerk and Director
  Senior Vice President of Engineering
  Vice President of Marketing
  Vice President of Research and Development
  Vice President of Finance, Chief Financial Officer,

Mr. Richard T. Schumacher – Mr. Schumacher’s biography can be found under the Directors heading.

Dr. Edmund Ting joined us as Senior Vice President of Engineering on April 24, 2006. Prior to joining us, Dr. Ting served as the Chief Research
Officer of Avure Technologies, a leading worldwide manufacturer of high pressure hydrostatic processing equipment for the food and materials
processing industry, where he worked from 2001 to 2006. From 1990 to 2001, Dr. Ting was employed by Flow International Corporation, a world
leader  in  the  ultrahigh  pressure  waterjet  cutting  technology  market,  and  the  parent  company  of Avure  Technologies  until  November  2005.  Dr.
Ting last held the position of Vice President of Engineering Research and Development at Flow International Corporation. From 1984 to 1990,
Dr. Ting was a research scientist and then a group leader at Grumman Aerospace Corporation. Dr. Ting earned a Bachelor of Science degree in
mechanical  engineering  from  Northeastern  University  and  a  Science  Doctorate  in  materials  science  and  engineering  from  the  Massachusetts
Institute of Technology.

Dr.  Nathan  P.  Lawrence   was  appointed  as  our  Vice  President  of  Marketing  and  Sales  on  April  1,  2006.    Dr.  Lawrence  joined  Pressure
BioSciences  Inc.  in  2005,  serving  as  Director  of  Research  and  Development  until  his  promotion  to  Vice  President  of  Marketing  in  2006.    Dr.
Lawrence  was  responsible  for  the  development  of  protocols  based  on  Pressure  Cycling  Technology  (PCT).    From  2004  through  2005,  Dr.
Lawrence worked for 454 Life Sciences Inc. in product development.  Prior to 454 Life Sciences, Dr. Lawrence was Director of Research and
Development  for  Boston  Biomedica,  Inc.  from  1998  to  2004.    He  was  primarily  responsible  for  the  development  of  PCT,  as  well  as  the
development  of  nucleic  acid-based  diagnostic  assays.    Prior  to  joining  Boston  Biomedica,  Inc.,  Dr.  Lawrence  held  several  positions  with
increasing responsibility in Research and Development and manufacturing at Becton Dickinson and Gene Trak Systems.  Dr. Lawrence holds a
BA from the University of Miami, an M.S. from Southern Connecticut State University, and a Ph.D. from Yale University.

Dr. Alexander Lazarev  has  served  as  our  Vice  President  of  Research  and  Development  since  2007.  Prior  to  that,  he  served  as  our  Director  of
Research and Development, since joining us in 2006. Prior to joining us, Dr. Lazarev worked as a Visiting Scientist at the Barnett Institute of
Chemical and Biological Analysis at Northeastern University in 2005, and served as a Director of New Technology Development at Proteome
Systems,  Inc.,  where  he  was  involved  in  research  and  development  of  innovative  proteomic  analysis  applications  from  2001  until  early  2006.
From 1998 to 2001, Dr. Lazarev was employed as Senior Scientist at the Proteomics Division of Genomic Solutions, Inc. Prior to his employment
at Genomic Solutions, Inc., Dr. Lazarev was employed in an analytical contract service startup company, PhytoChem Technologies, Inc., which
was founded as a spin-off from ESA, Inc. in 1997. Previously, Dr. Lazarev held various scientific positions at the Ohio State University School of
Medicine and the Uniformed Services University of Health Sciences. Most of his scientific career has been dedicated to development of methods
and applications for biochemical analysis. Since 2005, Dr. Lazarev has been elected as an Executive Board member of the MASSEP.org, a non-
profit scientific discussion forum dedicated to the promotion and improvement of chromatography and other analytical technologies. Dr. Lazarev
earned his undergraduate and graduate degrees at the University of Kazan, Russian Federation.

Mr. Joseph L. Damasio, Jr. has been our Vice President of Finance and Chief Financial Officer since April 2017. Mr. Damasio has over 20 years
of  finance  and  accounting  experience.  Prior  to  joining  PBI,  Mr.  Damasio  was  Finance  Director  at  Nelipak  Packaging,  a  private  equity  backed
medical  packaging  manufacturer  with  $130  million  in  global  annual  sales,  from April  2016  to April  2017.  Before  joining  Nelipak,  between
November 2012 and April 2016, Mr. Damasio held various financial positions at CP Bourg, IQE KC, and Kopin Corporation. Prior to Kopin, Mr.
Damasio  was  employed  by  PBI,  first  as Accounting  Manager  (2007),  then  as  Controller  (2008),  and  finally  as  Vice  President  of  Finance  and
Administration (2011). Prior to joining PBI in 2007, Mr. Damasio was a senior accountant at BearingPoint, Inc. (formerly KPMG Consulting)
and an auditor at PriceWaterhouseCoopers, LLP. Mr. Damasio began his financial career with NEN Life Science Products Inc., a subsidiary of
PerkinElmer Inc. Mr. Damasio earned a bachelor’s degree in accounting, with honors, from the University of Massachusetts. He holds an MBA
and MSF from Boston College and is a Certified Public Accountant in Massachusetts.

- 90 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Section 16(a) Beneficial Ownership Reporting Compliance

Section  16(a)  of  the  Exchange Act  requires  the  Company’s  executive  officers  and  directors,  and  persons  who  own  more  than  10%  of  the

Company’s common stock, to file reports of ownership and changes in ownership on Forms 3, 4 and 5 with the SEC.

Based solely on the Company’s review of the copies of such Forms and written representations from certain reporting persons, the Company
believes that all filings required to be made by the Company’s Section 16(a) reporting persons during the Company’s fiscal year ended December
31, 2017 were made on a timely basis with the exception of our officers, directors and greater than 10 percent beneficial owners listed in the table
below:

Name

Number of
Late Reports   Number and Description of Transactions Not Reported on a Timely Basis

Richard T. Schumacher

Kevin A. Pollack

Michael S. Urdea

Edmund Ting

Code of Ethics

1

1

1

1

  1  transaction  was  not  reported  on  a  timely  basis  following  the  acquisition  of  convertible

securities in the year ended December 31, 2017.

  1  transaction  was  not  reported  on  a  timely  basis  following  the  acquisition  of  convertible

securities in the year ended December 31, 2017.

  1  transaction  was  not  reported  on  a  timely  basis  following  the  acquisition  of  convertible

securities in the year ended December 31, 2017.

  1  transaction  was  not  reported  on  a  timely  basis  following  the  acquisition  of  convertible

securities in the year ended December 31, 2017.

Pursuant to Section 406 of the Sarbanes-Oxley Act of 2002, we have adopted a Code of Ethics for senior financial officers that applies to our
principal executive officer, principal financial officer, principal accounting officer, controller, and other persons performing similar functions. A
copy of the code of ethics is posted on, and may be obtained free of charge from our Internet website at http://www.pressurebiosciences.com. If
we make any amendments to this Code of Ethics or grant any waiver, including any implicit waiver, from a provision of this Code of Ethics to our
principal executive officer, principal financial officer, principal accounting officer, controller, or other persons performing similar functions, we
will  disclose  the  nature  of  such  amendment  or  waiver,  the  name  of  the  person  to  whom  the  waiver  was  granted  and  the  date  of  waiver  in  a
Current Report on Form 8-K.

Corporate Governance

Term of Office

Our directors are appointed for a three-year term to hold office until the annual general meeting of our shareholders or until removed

from office in accordance with our bylaws. Our officers are appointed by our board of directors and hold office until removed by the board.

Audit Committee

The Audit  Committee  was  established  in  accordance  with  Section  3(a)(58)(A)  of  the  Securities  Exchange Act  of  1934.  Messrs.  Pollack

(chairman), Mangiardi and Peterson are currently the members of the Audit Committee.

The Board of Directors has determined that Mr. Pollack qualifies as an “audit committee financial expert” as defined in Item 407(d)(5) of

Regulation S-K and is “independent” as defined by SEC and OTC Market rules.

The Audit Committee operates pursuant to a written charter (the “Audit Committee Charter”), a current copy of which is publicly available on
the investor relations portion of the Company’s website at www.pressurebiosciences.com. Under the provisions of the Audit Committee Charter,
the  primary  functions  of  the Audit  Committee  are  to  assist  the  Board  of  Directors  with  the  oversight  of  (i)  the  Company’s  financial  reporting
process,  accounting  functions,  and  internal  controls,  and  (ii)  the  qualifications,  independence,  appointment,  retention,  compensation,  and
performance of the Company’s independent registered public accounting firm. The Audit Committee is also responsible for the establishment of
“whistle-blowing” procedures, and the oversight of other compliance matters.

Compensation Committee

The  Board  of  Directors  has  a  Compensation  Committee,  consisting  of  Messrs.  Peterson,  Pollack  and  Mangiardi.  The  Compensation
Committee’s  duties  include  (i)  reviewing  and  approving  our  executive  compensation,  (ii)  reviewing  the  recommendations  of  the  president  and
chief executive officer regarding the compensation of our executive officers, (iii) evaluating the performance of the president and chief executive
officer, (iv) overseeing the administration and approval of grants of stock options and other equity awards under our equity incentive plans, and
(v) recommending compensation for our board of directors and each committee thereof for review and approval by the board of directors. The
Compensation Committee operates pursuant to a written charter, a current copy of which is publicly available on the investor relations portion of
our website at www.pressurebiosciences.com.

- 91 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Involvement in Certain Legal Proceedings

To the best of our knowledge, none of our directors or executive officers has, during the past ten years:

● been convicted  in  a  criminal  proceeding  or  been  subject  to  a  pending  criminal  proceeding  (excluding  traffic  violations  and  other minor

offenses);

● had any  bankruptcy  petition  filed  by  or  against  the  business  or  property  of  the  person,  or  of  any  partnership,  corporation  or business
association of which he was a general partner or executive officer, either at the time of the bankruptcy filing or within two years prior to
that time;

● been subject to any order, judgment, or decree, not subsequently reversed, suspended or vacated, of any court of competent jurisdiction or
federal or state authority, permanently or temporarily enjoining, barring, suspending or otherwise limiting, his involvement  in any type of
business, securities, futures, commodities, investment, banking, savings and loan, or insurance activities, or to be associated with persons
engaged in any such activity;

● been found by a court of competent jurisdiction in a civil action or by the Securities and Exchange Commission or the Commodity Futures
Trading Commission to have violated a federal or state securities or commodities law, and the judgment has not been reversed, suspended,
or vacated;

● been the  subject  of,  or  a  party  to,  any  federal  or  state  judicial  or  administrative  order,  judgment,  decree,  or  finding,  not  subsequently
reversed, suspended or vacated (not including any settlement of a civil proceeding among private litigants), relating to an alleged violation
of  any  federal  or  state  securities  or  commodities  law  or  regulation,  any  law  or  regulation  respecting  financial institutions  or  insurance
companies including, but not limited to, a temporary or permanent injunction, order of disgorgement or restitution, civil money penalty or
temporary or permanent cease-and-desist order, or removal or prohibition order, or any law or regulation prohibiting mail or wire fraud or
fraud in connection with any business entity; or

● been the  subject  of,  or  a  party  to,  any  sanction  or  order,  not  subsequently  reversed,  suspended  or  vacated,  of  any  self-regulatory
organization (as defined in Section 3(a)(26) of the Exchange Act), any registered entity (as defined in Section 1(a)(29) of  the Commodity
Exchange Act), or any equivalent exchange, association, entity or organization that has disciplinary authority over its members or persons
associated with a member.

Except as set forth in our discussion below in “Certain Relationships and Related Transactions,” none of our directors or executive officers
has been involved in any transactions with us or any of our directors, executive officers, affiliates or associates which are required to be disclosed
pursuant to the rules and regulations of the Commission.

- 92 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
ITEM 11. EXECUTIVE COMPENSATION

Executive Officer Compensation

Summary Compensation Table

The Summary Compensation Table below sets forth the total compensation paid or earned for the fiscal years ended December 31, 2017 and 2016
for: (i) each individual serving as our chief executive officer (“CEO”) or acting in a similar capacity during any part of fiscal 2017; and (ii) the
other two most highly paid executive officers (collectively, the “ Named Executive Officers”) who were serving as executive officers at the end of
fiscal 2017.

Name and Principal Position  

Fiscal
Year   Salary(1)     Bonus    

Stock
Awards    

Option
Awards(2)    

Non-Qualified
Deferred
Compensation
Earning

All other

Compensation(3)    Total

Richard T. Schumacher
President, CEO

  2017   $ 300,583    $
  2016     300,583     

Edmund Ting, Ph.D
Senior Vice President of
Engineering

  2017     207,480     
  2016     207,100     

Alexander Lazarev, Ph.D
Vice President of
Research and Development

  2017     173,880     
  2016     173,561     

-    $
-     

-     
-     

-     
-     

-    $ 142,285    $
-     
-     

-     
-     

31,303     
-     

-     
-     

28,457     
-     

-    $
-     

-     
-     

-     
-     

11,079    $ 453,947 
40,832      349,795 

1,227      240,010 
1,261      208,361 

7,739      210,076 
7,736      181,297 

(1) Salary refers to base salary compensation paid through  our  normal  payroll  process.  No  bonus  was  paid  to  any  named  executive  officer  for
2017 or 2016.

(2) Amounts shown do not reflect compensation received by the Named Executive Officers. Instead, the amounts shown are the aggregate grant
date fair value as determined pursuant to FASB ASC 718, Compensation-Stock Compensation. Please refer to Note 2, xiii, “Accounting for Stock-
Based  Compensation”  in  the  accompanying  Notes  to  Consolidated  Financial  Statements  for  the  fiscal  year  ended  December  31,  2017,  for  the
relevant assumptions used to determine the valuation of stock option grants.

(3) “All Other Compensation” includes our Company match to the executives’ 401(k) contribution and premiums paid on life insurance for the
executives. Both of these benefits are available to all of our employees. In the case of Mr. Schumacher, “All Other Compensation” also includes
$8,379 in premiums we paid for a life insurance policy to which Mr. Schumacher’s wife is the beneficiary. In 2016, Mr. Schumacher received
$29,708 for unused earned time off. “All Other Compensation” for Dr. Lazarev includes $6,000 paid to Dr. Lazarev in lieu of his participation in
the medical benefit plan offered by the Company.

- 93 -

 
 
 
 
 
 
   
 
 
 
 
   
     
     
     
     
     
     
 
 
 
 
   
      
      
      
      
      
      
  
 
 
   
      
      
      
      
      
      
  
 
 
 
   
      
      
      
      
      
      
  
 
 
   
      
      
      
      
      
      
  
 
 
 
 
 
 
 
 
Outstanding Equity Awards at Fiscal Year End

The following table sets forth certain information regarding outstanding stock options awards for each of the Named Executive Officers as of

December 31, 2017.

Name

Richard T. Schumacher
President, CEO

Edmund Y. Ting, Ph.D
Senior Vice President of
Engineering

Alexander V. Lazarev, Ph.D
Vice President of Research &
Development

Option Awards

Number of
Securities
Underlying
Unexercised
Options
Exercisable

Number of
Securities
Underlying
Unexercised
Options 
Unexercisable (1)

Option 
Exercise
Price ($)

Option
Expiration 
Date

2,500   
500   
1,000   
2,500   
10,000   
27,778   
6,250   

400   

1,400   
500   
584   
1,800   
6,667   
2,889   
1,375   

334   

1,167   
500   
500   
1,500   
5,000   
2,556   
1,250   

  $
- 
  $
- 
  $
- 
  $
- 
- 
  $
13,889(2)  $
18,750(3)  $

- 

  $

  $
- 
  $
- 
  $
- 
  $
- 
- 
  $
1,445(2)  $
4,125(3)  $

- 

  $

  $
- 
  $
- 
  $
- 
  $
- 
  $
- 
1,278(2)  $
3,750(3)  $

18.00   
30.00   
18.00   
12.00   
9.00   
12.00   
8.40   

30.00   

18.00   
30.00   
18.00   
12.00   
9.00   
12.00   
8.40   

30.00   

18.00   
30.00   
18.00   
12.00   
9.00   
12.00   
8.40   

3/12/2019
9/9/2021
3/13/2022
5/14/2023
9/24/2024
12/31/2025
3/16/2027

9/25/2018

3/12/2019
9/9/2021
3/13/2022
5/14/2023
9/24/2024
12/31/2025
3/16/2027

9/25/2018

3/12/2019
9/9/2021
3/13/2022
5/14/2023
9/24/2024
12/31/2025
3/16/2027

(1) All unvested stock options listed in this column were granted to the Named Executive Officer pursuant to our 2005 Equity Incentive Plan,
our  2013  Equity  Incentive  Plan,  and  our  2015  Non-Qualified  Stock  Option  Plan. All  options  expire  ten  years  after  the date  of  grant.
Unvested stock options become fully vested and exercisable upon a change of control of our company.

(2) Options to purchase shares of common stock were granted on December 31, 2015 to each of the Named Executive Officers, of which the

stock options will vest monthly from the date of grant over the three year vesting period.

(3) Options to purchase shares of common stock were granted on March 16, 2017 to each of the Named Executive Officers, of which the stock

options will vest monthly from the date of grant over the three year vesting period.

Retirement Plan

All employees, including the named executive officers, may participate in our 401(k) Plan. Under the 401(k) Plan, employees may elect to
make  before  tax  contributions  of  up  to  60%  of  their  base  salary,  subject  to  current  Internal  Revenue  Service  limits.  The  401(k)  Plan  does  not
permit  an  investment  in  our  common  stock.  We  match  employee  contributions  up  to  50%  of  the  first  2%  of  the  employee’s  earnings.  Our
contribution is 100% vested immediately.

- 94 -

 
 
 
 
 
 
 
 
 
   
 
 
   
 
 
   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
    
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
    
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Severance Arrangements

Each of Mr. Schumacher, Dr. Ting, Dr. Lazarev, and Dr. Lawrence, executive officers of the Company, are entitled to receive a severance
payment if terminated by us without cause. The severance benefits would include a payment in an amount equal to one year of such executive
officer’s annualized base salary compensation plus accrued paid time off. Additionally, the officer will be entitled to receive medical and dental
insurance coverage for one year following the date of termination.

Change-in-Control Arrangements

Pursuant to severance agreements with each of Mr. Schumacher, Dr. Ting, Dr. Lazarev and Dr. Lawrence, each such executive officers, is
entitled  to  receive  a  change  of  control  payment  in  an  amount  equal  to  one  year  (other  than  Mr.  Schumacher)  of  such  executive  officer’s
annualized base salary compensation, accrued paid time off, and medical and dental coverage, in the event of a change of control of our Company.
In the case of Mr. Schumacher, his payment is equal to two years of annualized base salary compensation, accrued paid time off, and two years of
medical and dental coverage.

Pursuant to our equity incentive plans, any unvested stock options held by a named executive officer will become fully vested upon a change

in control (as defined in the 2005 Equity Incentive Plan) of our Company.

Director Compensation and Benefits

The following table sets forth certain information regarding compensation earned or paid to our directors during fiscal 2017.

Name

Vito J. Mangiardi
Jeffrey N. Peterson
Kevin A. Pollack
Michael S. Urdea, Ph. D.

Fees Earned or

Paid in Cash ($) (1)    Stock Awards ($) (1)   
-   
-   
-   
-   

40,000   
60,000   
40,000   
40,000   

Option Awards ($)(2)
(3)

23,330   
38,884   
23,330   
14,693   

Total ($)

63,330 
98,884 
63,330 
54,693 

Our non-employee directors receive the following compensation for service as a director:

(1) Each director currently earns a quarterly stipend of $10,000 for attending meetings of the full board of directors (whether telephonic or in-
person) and attending committee meetings in 2017. Mr. Peterson currently earns $15,000 per quarter as chairman of the board of directors. There
is no limit to the number of board of directors or committee meetings that may be called.

(2) Amounts shown do not reflect compensation received by the directors. Instead, the amounts shown are the aggregate grant date fair value as
determined  pursuant  to  FASB  ASC  718,  Compensation-Stock  Compensation.  Please  refer  to  Note  2,  xiii,  “Accounting  for  Stock-Based
Compensation”  in  the  accompanying  Notes  to  the  Consolidated  Financial  Statements  for  the  fiscal  year  ended  December  31,  2017,  for  the
relevant assumptions used to determine the valuation of stock option grants.

(3)  The  following  table  shows  the  total  number  of  outstanding  stock  options  as  of  December  31,  2017  that  have  been  issued  as  director
compensation.

Name

Vito J. Mangiardi
Jeffrey N. Peterson
Kevin A. Pollack
Michael S. Urdea, Ph. D.

Aggregate
Number of Stock
Options
Outstanding

13,102 
22,578 
13,102 
10,185 

- 95 -

 
 
 
 
 
 
 
 
 
 
   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Report from Compensation Committee

General

Messrs. Peterson, Pollack and Mangiardi are currently the members of the Compensation Committee. The Compensation Committee operates
pursuant  to  a  written  charter,  a  current  copy  of  which  is  publicly  available  on  the  investor  relations  portion  of  our  website  at
www.pressurebiosciences.com.  The  primary  functions  of  the  Compensation  Committee  include  (i)  reviewing  and  approving  our  executive
compensation,  (ii)  reviewing  the  recommendations  of  the  president  and  chief  executive  officer  regarding  the  compensation  of  our  executive
officers, (iii) evaluating the performance of the president and chief executive officer, (iv) overseeing the administration and approval of grants of
stock options and other equity awards under our equity incentive plans, and (v) recommending compensation for our board of directors and each
committee thereof for review and approval by the board of directors.

The  Compensation  Committee  may  form  and  delegate  authority  to  one  or  more  subcommittees  as  it  deems  appropriate  from  time  to  time
under the circumstances (including (a) a subcommittee consisting of a single member and (b) a subcommittee consisting of at least two members,
each of whom qualifies as a “non-employee director,” as such term is defined from time to time in Rule 16b-3 promulgated under the Securities
Exchange Act of 1934, and an “outside director,” as such term is defined from time to time in Section 162(m) of the Internal Revenue Code of
1986, as amended, and the rules and regulations there under).

Compensation Objectives

In  light  of  the  relatively  early  stage  of  commercialization  of  our  products,  we  recognize  the  importance  of  attracting  and  retaining  key
employees  with  sufficient  experience,  skills,  and  qualifications  in  areas  vital  to  our  success,  such  as  operations,  finance,  sales  and  marketing,
research and development, engineering, and individuals who are committed to our short- and long-term goals. The Compensation Committee has
designed our executive compensation programs with the intent of attracting, motivating, and retaining experienced executives and, subject to our
limited financial resources, rewarding them for their contributions by offering them a competitive base salary, potential for annual cash incentive
bonuses, and long-term equity-based incentives, typically in the form of stock options. The Compensation Committee strives to balance the need
to  retain  key  employees  with  financial  prudence  given  our  history  of  operating  losses,  limited  financial  resources  and  the  early  stage  of  our
commercialization.

Executive Officers and Director Compensation Process

The Compensation Committee considers and determines executive compensation according to an annual objective setting and measurement
cycle. Specifically, corporate goals for the year are initially developed by our executive officers and are then presented to our board of directors
and Compensation Committee for review and approval. Individual goals are intended to focus on contributions that facilitate the achievement of
the corporate goals. Individual goals are first proposed by each executive officer, other than the president and CEO, then discussed by the entire
senior  executive  management  team  and  ultimately  compiled  and  prepared  for  submission  to  our  board  of  directors  and  the  Compensation
Committee, by the president and chief executive officer. The Compensation Committee sets and approves the goals for the president and chief
executive officer. Generally, corporate and individual goals are set during the first quarter of each calendar year. The objective setting process is
coordinated  with  our  annual  financial  planning  and  budgeting  process  so  our  board  of  directors  and  Compensation  Committee  can  consider
overall corporate and individual objectives in the context of budget constraints and cost control considerations. Annual salary increases, bonuses,
and equity awards, such as stock option grants, if any, are tied to the achievement of these corporate and individual performance goals as well as
our financial position and prospects.

Under  the  annual  performance  review  program,  the  Compensation  Committee  evaluates  individual  performance  against  the  goals  for  the
recently completed year. The Compensation Committee’s evaluation generally occurs in the first quarter of the following year. The evaluation of
each  executive  (other  than  the  president  and  chief  executive  officer)  begins  with  a  written  self-assessment  submitted  by  the  executive  to  the
president and chief executive officer. The president and chief executive officer then prepares a written evaluation based on the executive’s self-
assessment,  the  president  and  chief  executive  officer’s  evaluation,  and  input  from  others  within  the  Company.  This  process  leads  to  a
recommendation by the president and chief executive officer for a salary increase, bonus, and equity award, if any, which is then considered by
the Compensation Committee. In the case of the president and chief executive officer, the Compensation Committee conducts his performance
evaluation  and  determines  his  compensation,  including  salary  increase,  bonus,  and  equity  awards,  if  any.  We  generally  expect,  but  are  not
required, to implement salary increases, bonuses, and equity awards, for all executive officers, if and to the extent granted, by April 1 of each
year.

Non-employee  director  compensation  is  set  by  our  board  of  directors  upon  the  recommendation  of  the  Compensation  Committee.  In
developing  its  recommendations,  the  Compensation  Committee  is  guided  by  the  following  goals:  compensation  should  be  fair  relative  to  the
required services for directors of comparable companies in our industry and at our Company’s stage of development; compensation should align
directors’  interests  with  the  long-term  interest  of  stockholders;  the  structure  of  the  compensation  should  be  simple,  transparent,  and  easy  for
stockholders  to  understand;  and  compensation  should  be  consistent  with  the  financial  resources,  prospects,  and  competitive  outlook  for  the
Company.

- 96 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
In evaluating executive officer and director compensation, the Compensation Committee considers the practices of companies of similar size,
geographic  location,  and  market  focus.  In  order  to  develop  reasonable  benchmark  data  the  Compensation  Committee  has  referred  to  publicly
available  sources  such  as  www.salary.com  and  the  BioWorld  Survey.  While  the  Compensation  Committee  does  not  believe  benchmarking  is
appropriate as a stand-alone tool for setting compensation due to the unique aspects of our business objectives and current stage of development,
the  Compensation  Committee  generally  believes  that  gathering  this  compensation  information  is  an  important  part  of  its  compensation-related
decision making process.

The Compensation Committee has the authority to hire and fire advisors and compensation consultants as needed and approve their fees. No
advisors or compensation consultants were hired or fired in fiscal 2017. The Compensation Committee is also authorized to delegate any of its
responsibilities to sub committees or individuals as it deems appropriate. The Compensation Committee did not delegate any of its responsibilities
in fiscal 2017.

ITEM  12.  SECURITY  OWNERSHIP  OF  CERTAIN  BENEFICIAL  OWNERS  AND  MANAGEMENT  AND  RELATED
STOCKHOLDER MATTERS.

Beneficial Ownership Information

The following table sets forth certain information as of March 15, 2018 concerning the beneficial ownership of common stock for: (i) each
director and director nominee, (ii) each Named Executive Officer in the Summary Compensation Table under “Executive Compensation” above,
(iii) all executive officers and directors as a group, and (iv) each person (including any “group” as that term is used in Section 13(d)(3) of the
Exchange Act) known by us to be the beneficial owner of 5% or more of our common stock. The address for each of the persons below who are
beneficial owners of 5% or more of our common stock is our corporate address at 14 Norfolk Avenue, South Easton, MA 02375.

Beneficial  ownership  has  been  determined  in  accordance  with  the  rules  of  the  SEC  and  is  calculated  based  on  1,357,079  shares  of  our
common  stock  issued  and  outstanding  as  of  March  15,  2018.  Shares  of  common  stock  subject  to  options,  warrants,  preferred  stock  or  other
securities convertible into common stock that are currently exercisable or convertible, or exercisable or convertible within 60 days of March 15,
2018, are deemed outstanding for computing the percentage of the person holding the option, warrant, preferred stock, or convertible security but
are not deemed outstanding for computing the percentage of any other person.

Except as indicated by the footnotes below, we believe, based on the information furnished to us, that the persons and entities named in the

table below have sole voting and investment power with respect to all shares of common stock that they beneficially own.

Name of Beneficial Owner

Richard T. Schumacher(1)
Jeffrey N. Peterson(2)
Kevin A. Pollack(3)
Michael S. Urdea(4)
Vito J. Mangiardi(5)
Edmund Y. Ting, Ph.D(6)
Alexander V. Lazarev, Ph.D(7)
All other officers

Amount and 
Nature of 

Beneficial Ownership    
96,964   
44,093   
37,993   
31,618   
26,650   
17,523   
14,199   
18,812   

Percent of Class

6.83%
3.18%
2.75%
2.30%
1.94%
1.28%
1.04%
1.37%

All Executive Officers and Directors as a Group (8)

287,852   

18.52%

- 97 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
    
 
  
 
 
 
 
 
 
1)

2)

3)

4)

5)

Includes (i)  57,936  shares  of  Common  Stock  issuable  upon  exercise  of  options;  (ii)  2,620  shares  of  Common  Stock  issuable  upon
conversion of Convertible Debentures; (iii) 1,191 shares of Common Stock issuable upon the exercise of warrants and (iv) 35,217 shares
of  Common  Stock.  Does  not  include  672  shares  of  Common  Stock  held  by  Mr.  Schumacher’s  minor  son  as  Mr.  Schumacher’s  wife
exercises all voting and investment control over such shares.

Includes (i)  22,578  shares  of  Common  Stock  issuable  upon  exercise  of  options;  (ii)  5,500  shares  of  Common  Stock  issuable  upon
conversion of Convertible Debentures; (iii) 2,500 shares of Common Stock issuable upon the exercise of warrants; and (iv) 13,515 shares
of Common Stock.

Includes (i)  13,102  shares  of  Common  Stock  issuable  upon  exercise  of  options;  (ii)  6,112  shares  of  Common  Stock  issuable  upon
conversion of Convertible Debentures; (iii) 2,778 shares of Common Stock issuable upon the exercise of warrants and (iv) 16,001 shares
of Common Stock.

Includes (i)  10,185  shares  of  Common  Stock  issuable  upon  exercise  of  options;  (ii)  6,024  shares  of  Common  Stock  issuable  upon
conversion of Convertible Debentures; and (iii) 2,739 shares of Common Stock issuable upon the exercise of warrants; and (iv) 12,670
shares of Common Stock.

Includes (i)  13,102  shares  of  Common  Stock  issuable  upon  exercise  of  options;  (ii)  1,310  shares  of  Common  Stock  issuable  upon
conversion of  Convertible  Debentures;  and  (iii)  596  shares  of  Common  Stock  issuable  upon  the  exercise  of  warrants;  and  (iv)  11,642
shares of Common Stock.

6)

Includes (i) 16,708 shares of Common Stock issuable upon exercise of options and (ii) 815 shares of Common Stock.

7)

Includes (i) 13,789 shares of Common Stock issuable upon exercise of options and (ii) 410 shares of Common Stock.

8)

Includes (i)  165,448  shares  of  Common  Stock  issuable  upon  exercise  of  options;  (ii)  21,566  shares  of  Common  Stock  issuable  upon
conversion of Convertible Debentures; (iii) 9,804 shares of Common Stock issuable upon the exercise of warrants and (iv) 91,034 shares
of Common Stock.

Equity Compensation Plan Information

We  maintain  a  number  of  equity  compensation  plans  for  employees,  officers,  directors  and  other  entities  and  individuals  whose  efforts
contribute to our success. The table below sets forth certain information as of our fiscal year ended December 31, 2017 regarding the shares of
our common stock available for grant or granted under our equity compensation plans.

Plan Category

Equity compensation plan approved by security holders - 2005
Equity Incentive Plan
Equity compensation plan approved by security holders - 2013
Equity Incentive Plan
Equity compensation plan adopted by the Board of Directors -
2015 Non-Qualified Stock Option Plan

Number of
securities to be
issued upon 
exercise of
outstanding 
options

Weighted-average
exercise price of
outstanding 
options

Number of
securities
available for 
future issuance
under equity
compensation plans  

35,274    $

82,481    $

129,937    $

17.38   

9.38   

10.19   

0 

2,917,519 

4,870,063 

- 98 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
   
   
 
 
 
 
 
 
 
 
 
 
 
 
ITEM 13. CERTAIN RELATIONSHIPS AND RELATED TRANSACTIONS; AND DIRECTOR INDEPENDENCE.

The following is a summary of transactions since January 1, 2016 to which we have been or will be a party in which the amount involved
exceeded or will exceed $19,700 (one percent of the average of our total assets at year-end for our last two completed fiscal years) and in which
any of our directors, executive officers or beneficial holders of more than 5% of any class of our capital stock, or any immediate family member
of, or person sharing a household with, any of these individuals, had or will have a direct or indirect material interest, other than compensation
arrangements that are described under the section captioned “Executive compensation.”

During the year ended December 31, 2016, we received advances from certain officers of ours amounting to $20,000 and we repaid the loans.

These advances were non-interest bearing and were payable on demand.

On March 10, 2016, a relative of Mr. Schumacher invested $50,000 into the Company’s 2015/2016 convertible debenture financing. The loan
remains outstanding. The Holder was paid a 10% original investment discount (“OID”) for the first year, and will earn 10% annual interest during
the second year. The loan can be converted into common stock at any time by the Holder, at a fixed price of $0.28 per share. The loan is due two
years after the loan origination date.

On  March  23,  2016,  Mr.  Pollack  invested  $46,549  into  the  Company’s  2015/2016  convertible  debenture  financing.  The  loan  remains
outstanding. The Holder was paid a 10% original investment  discount  (“OID”)  for  the  first  year,  and  will  earn  10%  annual  interest  during  the
second year. The loan can be converted into common stock at any time by the Holder, at a fixed price of $0.28 per share. The loan is due two
years after the loan origination date.

On  March  31,  2016,  Mr.  Peterson  invested  $42,000  into  the  Company’s  2015/2016  convertible  debenture  financing.  The  loan  remains
outstanding. The Holder was paid a 10% original investment  discount  (“OID”)  for  the  first  year,  and  will  earn  10%  annual  interest  during  the
second year. The loan can be converted into common stock at any time by the Holder, at a fixed price of $0.28 per share. The loan is due two
years after the loan origination date.

On  March  31,  2016,  Mr.  Mangiardi  invested  $10,000  into  the  Company’s  2015/2016  convertible  debenture  financing.  The  loan  remains
outstanding. The Holder was paid a 10% original investment  discount  (“OID”)  for  the  first  year,  and  will  earn  10%  annual  interest  during  the
second year. The loan can be converted into common stock at any time by the Holder, at a fixed price of $0.28 per share. The loan is due two
years after the loan origination date.

On  March  31,  2016,  Mr.  Schumacher  invested  $20,000  into  the  Company’s  2015/2016  convertible  debenture  financing.  The  loan  remains
outstanding. The loan was paid a 10% original investment discount (“OID”) for the first year, and will earn 10% annual interest during the second
year. The loan can be converted into common stock at any time by the Holder, at a fixed price of $0.28 per share. The loan is due two years after
the loan origination date.

On March 31, 2016, a relative of Mr. Schumacher invested $30,000 into the Company’s 2015/2016 convertible debenture financing. The loan
remains outstanding. The loan was paid a 10% original investment discount (“OID”) for the first year, and will earn 10% annual interest during
the second year. The loan can be converted into common stock at any time by the Holder, at a fixed price of $0.28 per share. The loan is due two
years after the loan origination date.

On  March  31,  2016,  Dr.  Urdea  invested  $46,000  into  the  Company’s  2015/2016  convertible  debenture  financing.  The  loan  remains
outstanding. The loan was paid a 10% original investment discount (“OID”) for the first year, and will earn 10% annual interest during the second
year. The loan can be converted into common stock at any time by the Holder, at a fixed price of $0.28 per share. The loan is due two years after
the loan origination date.

- 99 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
In March 2010, we signed a strategic product licensing, manufacturing, co-marketing, and collaborative research and development agreement
with TDI. Under the terms of the agreement, we have been licensed by TDI to manufacture and sell a highly innovative line of chemicals used in
the  preparation  of  tissues  for  scientific  analysis  (“TDI reagents”).  The  TDI  reagents  were  designed  for  use  in  combination  with  our  pressure
cycling technology. The respective companies believe that the combination of PCT and the TDI reagents can fill an existing need in life science
research  for  an  automated  method  for  rapid  extraction  and  recovery  of  intact,  functional  proteins  associated  with  cell  membranes  in  tissue
samples. We did not incur any royalty obligation under this agreement in 2017 or 2016. We executed an amendment to this agreement on October
1, 2016 wherein we agreed to pay a monthly fee of $1,400 for the use of a lab bench, shared space and other utilities, and $2,000 per day for
technical support services as needed. Mr. Jeffrey N. Peterson, the chief executive officer of TDI, has served as a director of the Company since
July 2011 and as Chairman of the Board starting in 2012.

Board Independence

Our board of directors has reviewed the qualifications of each of Messrs. Peterson, Mangiardi, Pollack, and Dr. Urdea constituting more than
a majority of our directors and has affirmatively determined that each individual is “independent” as such term is defined under the current listing
standards of the OTC Markets. The board of directors has determined that none of these directors has a material relationship with us that would
interfere with the exercise of independent judgment. In addition, each member of the Audit Committee is independent as required under Section
10A(m)(3) of the Securities Exchange Act of 1934, as amended (the “Exchange Act”).

ITEM 14. PRINCIPAL ACCOUNTING FEES AND SERVICES

The Audit Committee appointed MaloneBailey LLP, an independent registered public accounting firm, to audit the Company’s consolidated

financial statements for the fiscal year ended December 31, 2017.

Independent Registered Public Accounting Fees

The  following  is  a  summary  of  the  fees  billed  to  the  Company  by  MaloneBailey  LLP,  the  Company’s  independent  registered  public

accounting firm, respectively for the fiscal year ended December 31, 2017 and 2016:

Audit Fees
Audit-Related Fees
Tax and Other Fees

Fiscal 2017 Fees

Fiscal 2016 Fees

$

$

118,549    $

-   
-   

118,549    $

107,156 
- 
- 
107,156 

Audit Fees. Consists of fees billed for professional services performed for the audit of our annual financial statements, the review of interim
financial  statements,  and  related  services  that  are  normally  provided  in  connection  with  registration  statements,  including  the  registration
statement for our public offering. Included in the 2017 Audit Fees is an aggregate of $26,500 of fees billed in connection with our public offering.

Audit-Related Fees. Consists of aggregate fees billed for assurance and related services that are reasonably related to the performance of the

audit or review of the Company’s consolidated financial statements and are not reported under “Audit Fees.”

Audit Committee Policy on Pre-Approval of Services

The Audit Committee’s policy is to pre-approve all audit and permissible non-audit services provided by the independent registered public
accounting  firm.  These  services  may  include  audit  services,  audit-related  services,  tax  services,  and  other  services.  Pre-approval  is  generally
provided for up to one year. The Audit Committee may also pre-approve particular services on a case-by-case basis.

- 100 -

 
 
 
 
 
 
 
 
 
 
 
   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
PART IV

ITEM 15. EXHIBITS AND FINANCIAL STATEMENT SCHEDULES.

Exhibit
Number
3.1
3.2

3.3

3.4

3.5

3.6

3.7

3.8

3.9

3.10

3.11

3.12
3.13

4.1

Exhibit Description

  Restated Articles of Organization of the Company.
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company
Articles of Amendment to Restated Articles of the
Organization of the Company

  Amended and Restated By-Laws of the Company

Amendment to Amended and Restated By-Laws of the
Company
Specimen Certificate for Shares of the Company’s common
stock

- 101 -

Incorporated by Reference

Form   Exhibit
S-1
10-Q

3.1
3.1

  Filing Date

10/08/1996  
11/23/2004

Filed or
Furnished
Herewith

8-K

8-K

8-K

8-K

8-K

8-K

8-K

8-K

8-K

S-1
10-K

10-KSB

3.1

3.1

3.1

3.1

3.1

3.1

3.1

3.1

3.1

3.2
3.3

4.1

02/18/2009

04/12/2011

11/10/2011

01/04/2013

02/13/2013

12/12/2013

02/05/2014

12/31/2014

07/28/2015

10/08/1996  
3/31/2003

04/22/2005

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Incorporated by Reference

Form   Exhibit
 8-K
 8-K
8-K
 10-K

4.1
4.2
4.1
10.11

  Filing Date

07/28/2015  
07/28/2015  
4/24/2017  
03/27/2008

 10-K

10.12

03/27/2008

 10-K

10.13

03/27/2008

 8-K
 8-K
 8-K
 S-8
 8-K
 10-K  
 10-K

 10-K

 S-8
10-K  
10-Q  
8-K
8-K
10-Q

10-Q  
8-K

8-K
8-K

10.1
10.2
10.1
99.1
10.1
10.5
10.6

10.7

4.1
10.13
10.1
10.1
10.2
10.1

10.2
10.1

10.2
10.1

07/28/2015  
07/28/2015  
11/03/2016  
09/26/2005  
09/29/2008  
03/27/2008  
03/27/2008

03/27/2008

04/24/2015  
03/22/2017 
11/14/2016  
04/24/2017  
04/24/2017  
05/15/2017

05/15/2017  
05/26/2017

05/26/2017  
12/18/2017

Filed or
Furnished
Herewith

X

X
X

X

X

X

X

Exhibit
Number
4.2
4.3
4.4
10.1

10.2

10.3

10.4
10.5
10.6
10.7
10.8
10.9
10.10

10.11

10.12
10.13
10.14
10.15
10.16
10.17

10.18
10.19

10.20
10.21

10.22

21.1
23.1

31.1

31.2

32.1

32.2

Exhibit Description

  Form of Debenture
  Form of Warrant
  Form of Debenture

Technology Transfer and Patent Assignment Agreement dated
October 7, 1996, between Bioseq, Inc. and BioMolecular
Assays, Inc.
Amendment to Technology Transfer and Patent Assignment
Agreement dated October 8, 1998 between Bioseq, Inc. and
BioMolecular Assays, Inc.
Nonexclusive License Agreement dated September 30, 1998
between Bioseq, Inc. and BioMolecular Assays, Inc.

  Subscription Agreement
  Security Agreement
  Promissory Note, dated October 26, 2016

2005 Equity Incentive Plan.*

  Amendment No. 1 to 2005 Equity Incentive Plan*
  Description of Compensation for Certain Directors*

Severance Agreement between the registrant and Richard T.
Schumacher*
Form of Severance Agreement including list of officers to
whom provided*
2013 Equity Incentive Plan.*
2015 Nonqualified Stock Option Plan.*

  Securities Purchase Agreement
  Securities Purchase Agreement, dated March 14, 2017
  Letter Agreement, dated April 19, 2017

Amendment to the July 1, 2016 $200,000 Convertible Note
between Vision Capital and Pressure BioSciences, Inc.
  Securities Purchase Agreement dated March 14, 2017

Amendment Number 1 to October 26 Promissory Note, dated
May 2, 2017

  Promissory Note, dated May 19, 2017

Asset Purchase Agreement between Pressure BioSciences,
Inc. and BaroFold, Inc., dated December 12, 2017.
Amendment Number 2 to October 26 Promissory Note, dated
May 2, 2017

  List of Subsidiaries

Consent of Independent Registered Public Accounting Firm
(Malone Bailey LLP)
Principal Executive Officer Certification Pursuant to Item
601(b)(31) of Regulation S-K, as adopted pursuant to Section
302 of the Sarbanes-Oxley Act of 2002.
Principal Financial Officer Certification Pursuant to Item
601(b)(31) of Regulation S-K, as adopted pursuant to Section
302 of the Sarbanes-Oxley Act of 2002.
Principal Executive Officer and Principal Financial Officer
Certification Pursuant to Item 601(b)(32) of Regulation S-K,
as adopted pursuant to Section 906 of the Sarbanes-Oxley Act
of 2002.**
Principal Executive Officer and Principal Financial Officer
Certification Pursuant to Item 601(b)(32) of Regulation S-K,
as adopted pursuant to Section 906 of the Sarbanes-Oxley Act
of 2002.**

*Management contract or compensatory plan or arrangement.

**In accordance with SEC Release 33-8238, Exhibit 32.1 is furnished and not filed.

- 102 -

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Pursuant to the requirements of Section 13 or 15(d) of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed
on its behalf by the undersigned, thereunto duly authorized.

Date: April 2, 2018

Pressure BioSciences, Inc.

SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934, this report has been signed below by the following persons on behalf of

the registrant and in the capacity and on the dates indicated.

By:

/s/ Richard T. Schumacher
Richard T. Schumacher
President and Chief Executive Officer

Name

Capacity

/s/ Richard T. Schumacher
Richard T. Schumacher

President, Chief Executive Officer, Treasurer, Clerk and Director
(Principal Executive Officer)

Date

April 2, 2018

April 2, 2018

/s/ Joseph L. Damasio
Joseph L. Damasio

/s/ Jeffrey N. Peterson
Jeffrey N. Peterson

/s/ Mickey Urdea
Michael S.Urdea, Ph.D.

/s/ Vito Mangiardi
Vito J. Mangiardi

/s/ Kevin Pollack
Kevin A. Pollack

Chief Financial Officer
(Principal Financial Officer)

Chairman of the Board of Directors

April 2, 2018

Director

Director

Director

- 103 -

April 2, 2018

April 2, 2018

April 2, 2018

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
NEITHER THE ISSUANCE AND SALE OF THE SECURITIES REPRESENTED BY THIS PROMISSORY NOTE NOR THE
SECURITIES  INTO  WHICH  THESE  SECURITIES  ARE  EXERCISABLE  HAVE  BEEN  REGISTERED  UNDER  THE
SECURITIES ACT OF 1933, AS AMENDED, OR APPLICABLE STATE SECURITIES LAWS. THE SECURITIES MAY NOT
BE  OFFERED  FOR  SALE,  SOLD,  TRANSFERRED  OR  ASSIGNED  (I)  IN  THE  ABSENCE  OF  (A)  AN  EFFECTIVE
REGISTRATION STATEMENT FOR THE SECURITIES UNDER THE SECURITIES ACT OF 1933, AS AMENDED, OR (B)
AN  OPINION  OF  COUNSEL  (WHICH  COUNSEL  SHALL  BE  SELECTED  BY  THE  HOLDER),  IN  A  GENERALLY
ACCEPTABLE FORM, THAT REGISTRATION IS NOT REQUIRED UNDER SAID ACT OR (II) UNLESS SOLD PURSUANT
TO RULE 144 OR RULE 144A UNDER SAID ACT. NOTWITHSTANDING THE FOREGOING, THE SECURITIES MAY BE
PLEDGED  IN  CONNECTION  WITH  A  BONA  FIDE  MARGIN  ACCOUNT  OR  OTHER  LOAN  OR  FINANCING
ARRANGEMENT SECURED BY THE SECURITIES.

PRESSURE BIOSCIENCES, INC.

AMENDMENT NUMBER 2 TO OCTOBER 26, 2016 PROMISSORY NOTE

Original Principal: US$2,000,000

Original Issue Date: October 26, 2016

Amendment No. 1
Principal: US$3,000,000

Amendment No. 2
Principal: US$3,500,000

Amendment No. 1 Issue Date: May 2, 2017

Amendment No. 2 Issue Date: August 18, 2017

WHEREAS,  PRESSURE  BIOSCIENCES,  INC.,  a  corporation  incorporated  under  the  laws  of  the  Commonwealth  of
Massachusetts and located at 14 Norfolk Avenue, South Easton, MA 02375 (the “ Company”)  previously  issued  a  Promissory  Note  (the
“Original Note”) on October 26, 2016 (the “Original Issue Date”), in favor of XXXXX, an individual residing at 175 West Jackson Blvd,
Suite 400, Chicago, IL 60091 (the “Holder”) for the principal sum of Two Million United States Dollars (US$2,000,000);

WHEREAS, the Company and the Holder amended the Original Note on May 2, 2017 (the “Amendment Number 1 Issue Date”),
to, among other amendments, increase the principal sum to Three Million United States Dollars (US$3,000,000) (“Amendment  Number
1”); and

WHEREAS, the Company and the Holder wish to again amend the Original Note with such amendment having an issue date of
August 18, 2017 (this “Amendment Number 2”), the Original Note having the Original Issue Date, and Amendment Number 1 having the
Amendment Number 1 Issue Date.

 
 
 
 
 
 
 
 
 
 
 
 
 
NOW,  THEREFORE,  in  consideration  of,  among  other  things,  the  premises,  representations,  respective  covenants  and

agreements contained herein, each party hereby agrees to the following:

1. Capitalized terms used, but not defined, herein, shall have the meanings ascribed to such terms in the Original Note as amended

by Amendment Number 1.

2. The definition of Principal Amount in the Original Note as amended by Amendment Number 1 is changed to Three Million

Five Hundred United States Dollars (US$3,500,000).

3. All other terms and conditions of the Original Note as amended by Amendment Number 1 shall remain in full force and effect.

4. This Amendment Number 2 may be executed in any number of counterparts and by the different signatories hereto on separate
counterparts, each of which, when so executed, shall be deemed an original, but all such counterparts shall constitute but one
and the same instrument. This Amendment Number 2 may be executed by facsimile transmission, PDF, electronic signature or
other similar electronic means with the same force and effect as if such signature page were an original thereof.

[Signature page follows]

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
IN  WITNESS  WHEREOF,  this Amendment  Number  2  has  been  executed  and  delivered  on  the Amendment  No.  2  Issue  Date

specified above.

COMPANY:

PRESSURE BIOSCIENCES, INC.

Richard T. Schumacher
President and CEO

By:
Name:
Title:

HOLDER:

XXXXXX

[signature page to Amendment Number 2]

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Pressure BioSciences, Inc. – Subsidiaries

PBI BioSeq, Inc. (U.S.A.)
Pressure BioSciences Europe (Poland)

EXHIBIT 21.1

 
 
 
 
 
 
 
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in the Registration Statements on Form S-8 (File Nos. 333-30320, 333-24749, 333-128594,
333-155405 and 333-203609) of our report dated April 2, 2018, with respect to the audited consolidated financial statements of Pressure
BioSciences,  Inc.,  which  is  included  in  this Annual  Report  on  Form  10-K  as  of  and  for  the  year  ended  December  31,  2017.  Our  report
contains an explanatory paragraph regarding the Company’s ability to continue as a going concern.

EXHIBIT 23.1

/s/ Malone Bailey LLP
www.malonebailey.com
Houston, Texas
April 2, 2018

 
 
 
 
 
 
 
 
 
 
 
 
CERTIFICATION PURSUANT TO SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002

EXHIBIT 31.1

I, Richard T. Schumacher, certify that:

1. I have reviewed this report on Form 10-K of Pressure BioSciences, Inc.;

2. Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to
make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period
covered by this report;

3. Based on my knowledge, the financial statements, and other financial information included in this report, fairly present in all material
respects the financial condition, results of operations and cash flows of the registrant as of, and for, the periods presented in this report;

4.  The  registrant’s  other  certifying  officer  and  I  are  responsible  for  establishing  and  maintaining  disclosure  controls  and  procedures  (as
defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) and internal control over financial reporting (as defined in Exchange Act Rule 13a-
15(f) and 15d-15(f)) for the registrant and have:

a)  Designed  such  disclosure  controls  and  procedures,  or  caused  such  disclosure  controls  and  procedures  to  be  designed  under  our
supervision,  to  ensure  that  material  information  relating  to  the  registrant,  including  its  consolidated  subsidiary,  is  made  known  to  us  by
others within those entities, particularly during the period in which this report is being prepared;

b) Designed such internal control over financial reporting, or caused such internal control over financial reporting to be designed under our
supervision, to provide reasonable assurance regarding the reliability of financial reporting and the preparation of financial statements for
external purposes in accordance with generally accepted accounting principles;

c) Evaluated the effectiveness of the registrant’s disclosure controls and procedures and presented in this report our conclusions about the
effectiveness of the disclosure controls and procedures, as of the end of the period covered by this report based on such evaluation; and

d) Disclosed in this report any change in the registrant’s internal control over financial reporting that occurred during the registrant’s most
recent fiscal quarter (the registrant’s fourth fiscal quarter in the case of an annual report) that has materially affected, or is reasonably likely
to materially affect, the registrant’s internal control over financial reporting; and

5.  The  registrant’s  other  certifying  officer  and  I  have  disclosed,  based  on  our  most  recent  evaluation  of  internal  control  over  financial
reporting, to the registrant’s auditors and the audit committee of the registrant’s board of directors (or persons performing the equivalent
functions):

a) All  significant  deficiencies  and  material  weaknesses  in  the  design  or  operation  of  internal  control  over  financial  reporting  which  are
reasonably likely to adversely affect the registrant’s ability to record, process, summarize and report financial data; and

b) Any fraud, whether or not material, that involves management or other employees who have a significant role in the registrant’s internal
control over financial reporting.

Date: April 2, 2018

/s/ Richard T. Schumacher

By:
Name: Richard T. Schumacher
Title:

President and Chief Executive Officer
(Principal Executive Officer)

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
CERTIFICATION PURSUANT TO SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002

EXHIBIT 31.2

I, Joseph L. Damasio, certify that:

1. I have reviewed this report on Form 10-K of Pressure BioSciences, Inc.;

2. Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to
make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period
covered by this report;

3. Based on my knowledge, the financial statements, and other financial information included in this report, fairly present in all material
respects the financial condition, results of operations and cash flows of the registrant as of, and for, the periods presented in this report;

4.  The  registrant’s  other  certifying  officer  and  I  are  responsible  for  establishing  and  maintaining  disclosure  controls  and  procedures  (as
defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) and internal control over financial reporting (as defined in Exchange Act Rule 13a-
15(f) and 15d-15(f)) for the registrant and have:

a)  Designed  such  disclosure  controls  and  procedures,  or  caused  such  disclosure  controls  and  procedures  to  be  designed  under  our
supervision,  to  ensure  that  material  information  relating  to  the  registrant,  including  its  consolidated  subsidiary,  is  made  known  to  us  by
others within those entities, particularly during the period in which this report is being prepared;

b) Designed such internal control over financial reporting, or caused such internal control over financial reporting to be designed under our
supervision, to provide reasonable assurance regarding the reliability of financial reporting and the preparation of financial statements for
external purposes in accordance with generally accepted accounting principles;

c) Evaluated the effectiveness of the registrant’s disclosure controls and procedures and presented in this report our conclusions about the
effectiveness of the disclosure controls and procedures, as of the end of the period covered by this report based on such evaluation; and

d) Disclosed in this report any change in the registrant’s internal control over financial reporting that occurred during the registrant’s most
recent fiscal quarter (the registrant’s fourth fiscal quarter in the case of an annual report) that has materially affected, or is reasonably likely
to materially affect, the registrant’s internal control over financial reporting; and

5.  The  registrant’s  other  certifying  officer  and  I  have  disclosed,  based  on  our  most  recent  evaluation  of  internal  control  over  financial
reporting, to the registrant’s auditors and the audit committee of the registrant’s board of directors (or persons performing the equivalent
functions):

a) All  significant  deficiencies  and  material  weaknesses  in  the  design  or  operation  of  internal  control  over  financial  reporting  which  are
reasonably likely to adversely affect the registrant’s ability to record, process, summarize and report financial data; and

b) Any fraud, whether or not material, that involves management or other employees who have a significant role in the registrant’s internal
control over financial reporting.

Date: April 2, 2018

/s/ Joseph L. Damasio

By:
Name: Joseph L. Damasio
Title: Chief Financial Officer

(Principal Financial Officer)

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Certification
Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002
(Subsections (a) and (b) of Section 1350, Chapter 63 of Title 18, United States Code)

EXHIBIT 32.1

In connection with the Annual Report on Form 10-K of Pressure BioSciences, Inc., a Massachusetts corporation (the “Company”) for
the period ended December 31, 2017 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), Richard T.
Schumacher,  President  and  Chief  Executive  Officer,  of  Pressure  BioSciences,  Inc.,  a  Massachusetts  corporation  (the  “Company”),  do
hereby certify, pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 (subsections (a) and (b) of Section 1350, Chapter 63 of Title 18,
United States Code) that:

(1) The Report of the Company fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934;

and

(2) The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of

the Company.

Dated: April 2, 2018

/s/ Richard T. Schumacher
Richard T. Schumacher
President and Chief Executive Officer
(Principal Executive Officer)

A signed original of this written statement required by Section 906 has been provided to Pressure BioSciences, Inc., and will be retained by
Pressure BioSciences, Inc. and furnished to the Securities and Exchange Commission or its staff upon request.

 
 
 
 
 
 
 
 
 
 
 
 
 
Certification
Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002
(Subsections (a) and (b) of Section 1350, Chapter 63 of Title 18, United States Code)

EXHIBIT 32.2

In connection with the Annual Report on Form 10-K of Pressure BioSciences, Inc., a Massachusetts corporation (the “Company”) for
the period ended December 31, 2017 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), Joseph L.
Damasio, Chief Financial Officer, of Pressure BioSciences, Inc., a Massachusetts corporation (the “Company”), do hereby certify, pursuant
to Section 906 of the Sarbanes-Oxley Act of 2002 (subsections (a) and (b) of Section 1350, Chapter 63 of Title 18, United States Code)
that:

(1) The Report of the Company fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934;

and

(2) The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of

the Company.

Dated: April 2, 2018

/s/ Joseph L. Damasio
Joseph L. Damasio
Chief Financial Officer
(Principal Financial Officer)

A signed original of this written statement required by Section 906 has been provided to Pressure BioSciences, Inc., and will be retained by
Pressure BioSciences, Inc. and furnished to the Securities and Exchange Commission or its staff upon request.