Quarterlytics / Healthcare / Biotechnology / ProQR Therapeutics N.V.

ProQR Therapeutics N.V.

prqr · NASDAQ Healthcare
Claim this profile
Ticker prqr
Exchange NASDAQ
Sector Healthcare
Industry Biotechnology
Employees 51-200
← All annual reports
FY2018 Annual Report · ProQR Therapeutics N.V.
Sign in to download
Loading PDF…
ANNUAL
REPORT
2018

Future medicines in sight

UNMET NEED IN RARE DISEASES

AN EYE ON 
THE GOAL

At ProQR, we focus on areas where there remains significant unmet need for 

new treatments. For most people living with rare genetic diseases, there are no 

therapies available. This may be due to the rarity of their disease or difficulties 

in treating with conventional methods. We hold a strong belief that people 

with rare diseases should enjoy a high quality of life and be able to enjoy it 

with their loved ones. Over the last several years, this goal has brought many 

good things to us, and the people we aim to help using our RNA therapies.  

In this magazine, we want to highlight what we have worked on and achieved  

in 2018, but also provide a glimpse into what 2019 will bring. 

In recent years we have focused 

developed for people with Leber’s 

more on eye diseases. The reason 

congenital amaurosis 10 (LCA10).  

for this is two-fold as most genetic 

LCA is the most common genetic 

eye diseases don’t have a treatment 

cause of childhood blindness of 

and RNA therapies are well suited to 

which LCA10 is one of the most 

treat these types of diseases.  

severe forms affecting about 2,000 

This focus has yielded results such  

patients in the Western world.  

as our positive clinical data for  

In LCA10 a process called photo- 

sepofarsen (formerly named  

transduction is disrupted in the light 

QR-110). Sepofarsen is being  

detecting cells (photoreceptor cells) 

Every 
minute 
of every 
day, TEN 
CHILDREN 
AROUND 
THE WORLD 
are born 
with a rare 
genetic 
disease

PAGE II
Ma ga zine 
ANNUAL REP ORT 2018

in the retina, due to a mistake in the 

implants that help mitigate most 

CEP290 gene. The mistake, or mu-

of the hearing loss these patients 

tation, changes the CEP290 protein 

experience. We are proud that 2018 

so that it cannot function properly 

saw the start of preclinical testing 

causing poor vision and blindness. 

of QR-421a, and even more proud 

Sepofarsen is our effort to repair  

that we partnered with Foundation 

the mutation in the RNA, resulting 

Fighting Blindness (FFB). The $7.5 of 

in a normal protein to improve 

million funding provided by FFB for 

vision. In September of 2018, we 

the clinical development of QR-421a 

presented interim results from the 

is more than just monetary support 

ongoing Phase 1/2 clinical study 

but a vote of confidence in our 

demonstrating that most partici-

patient-centric drug development 

pants experienced vision improve-

approach. We aim to publish interim 

ment following treatment with 

results from the Phase 1/2 clinical 

sepofarsen. It’s really encouraging 

study of QR-421a, called STELLAR,  

to see beneficial results for patients 

in 2019. 

in the first study, which we plan to 

finish in 2019. Additionally, we are 

We are also working on another 

also planning to start a Phase 2/3 

therapy for RP, caused by a different 

clinical study for sepofarsen called 

mutation called autosomal dominant 

ILLUMINATE. The ILLUMINATE trial 

retinitis pigmentosa (adRP). This 

has the potential to be the final study 

disease is related to the light-

required for market approval for 

sensitive protein rhodopsin, which 

sepofarsen. Read more about this in 

transforms light into neural signals 

our interview with Dr. David Rodman, 

for the brain. In patients with adRP 

Executive Vice President of Research 

this function is impaired. Our drug 

& Development at ProQR.

candidate, QR-1123, aims to block 

the formation of the mutated 

Another big development is our  

rhodopsin protein, potentially 

investigational therapy for Usher 

stopping or reversing vision loss.  

syndrome type 2. Usher syndrome 

QR-1123, which was initially 

is a devastating disease combining 

discovered by Ionis pharmaceuticals, 

deafness and blindness. There are 

was obtained by ProQR with an 

three types, which are determined 

exclusive worldwide license. In 2019, 

by the severity of hearing loss,  

we are planning to start a Phase 1/2 

the presence or absence of balance 

clinical study for QR-1123.

problems, and the age of onset of 

symptoms. Type 2 is caused by  

Butterfly Wings 

a DNA mutation that results in a 

While we are excited about our  

lack of usherin protein, resulting 

progress in ophthalmic diseases,  

in retinitis pigmentosa (RP) that 

it’s by no means the only path we 

disrupts eye function. Our drug  

are exploring. The effort we started 

QR-421a circumvents the mutation 

in dystrophic epidermolysis bullosa 

by skipping over the mutated 

(DEB) is continuing to progress. DEB 

part of the gene. This generates a 

is one of the more severe forms of 

shorter yet still functional usherin 

EB, caused by a weak connection  

protein, with the aim to stop or even 

between the dermis (inner layer) 

reverse the vision loss. As a therapy, 

and epidermis (outer layer) of the 

it would complement the cochlear 

skin. This connection is weakened 

PAG E III
Magazi ne 
ANNUAL R EPORT 2018

PAGE IV
Ma ga zine 
ANNUAL REP ORT 2018

by a DNA mutation that inhibits  

started the first clinical study 

$5 million from EB Research 

the connective function of the 

(Phase 1/2) for QR-313, called 

Partnership and EB Medical 

protein collagen (type VII). It results 

WINGS. A newly formed company, 

Research Foundation to develop 

in the blistering of skin and mucosal 

Wings Therapeutics, dedicated to 

QR-313 for patients with dystrophic 

membranes. Those affected have 

developing therapies for DEB will 

epidermolysis bullosa. 

to live with constant pain, infections 

continue development of QR-313 

RESEARCH AND DEVELOPMENT PIPELINE – DECEMBER 2018

Ophthalmology

and risk of malnutrition. Because 

and complete the WINGS study.

In September, we finalized a success- 

DISCOVERY

PRECLINICAL DEVELOPMENT

their skin is as fragile as a butterfly, 

children with DEB are sometimes 

Financial sustainability

ful public offering of ProQR shares. 

We sold 6,612,500 shares at $15.75 

called ‘butterfly children.’ It’s a 

Looking back at 2018, there are also 

per share, raising approximately 

heart-breaking disease and so  

financial reasons to be encouraged 

$104 million. And to close the year, 

far only palliative treatments  

with our progress. We started the 

in December we were granted  

are available. 

year with €48.1 million in cash and 

€5 million from the Dutch 

throughout the year have been  

government as innovation credit 

At ProQR we discovered an 

successful in raising capital to 

for the development of sepofarsen. 

investigational drug called QR-313 

fund the development of all the 

With all this, we ended the year with 

for a subgroup of DEB patients. 

programs that can potentially have  

€105.6 million in cash, which will 

By skipping the disease-causing 

a meaningful impact on patients.  

fund the development of four of 

mutation, a shorter but functional 

At the beginning of 2018 we 

more clinical programs, including 

collagen protein is expected to 

received the already mentioned 

the potentially pivotal trial for 

Sepofarsen (QR-110) for LCA10 p.Cys998X

QR-421a for Usher syndrome 2A exon 13

QR-1123 for P23H adRP - discovered by Ionis

QR-504 for FECD3

be formed. This should result 

$7.5 million from the Foundation 

sepofarsen and advance our early 

QR-411 for Usher syndrome 2A PE-40

in better wound healing and 

Fighting Blindness. In addition 

stage pipeline, providing a cash 

prevent blistering. In 2018, we 

we were granted approximately 

runway into 2021. 

PROOF OF 
CONCEPT TRIALS

LATE STAGE/
REGISTRATIONAL 
TRIALS

5-YEAR PLAN: ProQR’S VISION2023

We have always set ambitious goals to help as many patients 

programs in our pipeline. In order to bring our medicines to 

as possible using our RNA therapies. In the coming five years 

patients as efficiently as possible we intend to independently 

our aim is to build out our platform for the discovery and 

commercialize the eye programs in our pipeline. Most of our 

development of programs in eye diseases in order to have 

pipeline will be focused on eye diseases because we think 

marketing approval for at least two programs and have three 

this is where we can have the biggest impact, but we plan to 

additional programs in late stage clinical studies by the end of 

also develop programs in other areas where there is a strong 

2023. Beyond that we plan to have at least seven additional 

need for new therapies, such as skin and brain diseases. 

QR-1011 for Stargardt’s disease c.5461-10T>C

QRX-461 for Usher syndrome undisclosed mutation

QRX-136 for LCA undisclosed mutation

Beyond ophthalmology

DISCOVERY

PRECLINICAL DEVELOPMENT

PROOF OF 
CONCEPT TRIALS

LATE STAGE/
REGISTRATIONAL 
TRIALS

QRX-704 for Huntington’s Disease

2

COMMERCIAL 
PRODUCTS

3

LATE STAGE 
PROGRAMS 

7

EARLY STAGE 
PROGRAMS

For the latest developments in our pipeline visit www.proqr.com/pipeline/ 
or scan the QR-code on the back of this magazine 

PAG E V
Magazi ne 
ANNUAL R EPORT 2018

PAGE VI
Ma ga zine 
ANNUAL REP ORT 2018

AT A GLANCE:

WHAT ARE 
RNA THERAPIES?

RNA therapies: it’s what we do, but it can sometimes sound complicated.  

If you want to learn more – keep reading. Here we’ll explain how cells function, 

and how RNA therapy can intervene in genetic diseases. 

ProQR RNA MEDICINE PLATFORM 
(human cell)

Healthy
Healthy

Disease
Disease

Treated
Treated

Protein

Protein

Protein

RNA

DNA

RNA

DNA

RNA THERAPY

RNA

DNA

Cell
Nucleus

Cell
Nucleus

Cell
Nucleus

Check out the video on www.proqr.com/we-are-proqr/ if you want to learn more

OUR TOOLBOX OF RNA THERAPIES

At ProQR, we use different types of RNA therapies, 

Mutant-specific knockdown

depending on what type of mutation causes a disease. 

People inherit two copies of each gene, one from each  

Together, these technologies form our ‘toolbox’ to 

of their parents. Sometimes, a mutation in one version  

repair RNA. Here they are explained in a nutshell.

of the gene leads to a toxic protein that causes a disease.  

Splice correction

It is possible to design an RNA therapy that only destroys 

(knocks down) the mutated version of the RNA. The toxic 

Sometimes a mutation causes the cell to incorrectly splice  

protein is therefore not made and the cause of the disease is 

the RNA, either leaving behind parts of RNA that should 

removed. This is how QR-1123 for adRP is designed to work.

have been cut out or cutting out parts that should have 

remained. An RNA therapy can be designed to ‘hide’  

Repeat targeting

the mutation that allows the cell to splice the RNA 

Sometimes a mutation causes a stretch of DNA to be  

correctly and restore the function of the protein. This is 

copied a few times, these copies are also present in  

DNA lies at the core of normal  

Genetic diseases are caused by 

Using RNA therapies, which are 

how sepofarsen for LCA10, QR-411 for Usher syndrome 

the RNA. This is called a ‘repeat’, and may cause disease  

cellular function. It contains  

mistakes, or mutations, in the DNA. 

basically short pieces of RNA made 

and QR-1011 for Stargardt’s disease are designed to work.

by creating a toxic RNA or protein. An RNA therapy  

genes, which are the genetic  

These mutations are copied  

in a laboratory, we aim to repair  

instructions on how to make  

into the RNA blueprint, which 

the RNA blueprint to restore the 

Exon skipping

can be designed that targets and blocks the toxic function  

of these repeats to prevent their negative effect.  

the functional building blocks of 

means the protein is also not made 

function of the protein. This way, 

A mutation that causes a disease can sometimes be repaired 

This is how QR-504 for FECD3 is designed to work.

the cell, such as proteins. However 

correctly. Malfunctioning proteins 

we can target the underlying cause 

using the exon skipping method. An RNA therapy can be 

to get from the DNA to protein, 

can cause cells to not function 

of the disease without making 

designed to cause the cell to ‘skip’ the part of the RNA (the 

RNA editing

the information in the DNA is first 

properly causing the disease.  

permanent changes to the DNA. 

exon) that contains the mutation so that the rest of the 

Scientists at ProQR have developed a completely novel 

copied into RNA. The RNA acts as 

For example, if an essential protein 

Scientists around the world, includ-

RNA can still make a protein. The protein is a little shorter 

technique to repair RNA. An RNA therapy can be 

the blueprint for making proteins. 

is not working in the eye this may 

ing those at ProQR, have developed 

than normal, but can still perform its function. This is 

designed to actively recruit the cell’s own RNA editing 

Proteins are responsible for making 

lead to blindness. 

a toolbox of novel ways to repair 

how QR-421a for Usher syndrome is designed to work.

system which can edit certain mutations in the RNA 

sure that the cells in your body 

RNA. As a result, depending on the 

function normally. 

We take an innovative approach  

type of mutation, we can choose 

to treating genetic diseases.  

the best tool to do the job. 

back to its healthy form. This technique is used for 

ProQR’s Axiomer® platform that can potentially be used 

to repair thousands of disease causing mutations. 

PAGE VIII
Ma ga zine 
ANNUAL REP ORT 2018

It has been roughly two years since David Rodman became the Executive 

Vice-President Research & Development at ProQR. He was appointed to 

apply his knowledge of study design and genetic diseases on our pipeline. 

In September of 2018, we were able to present positive interim results from 

the clinical study for sepofarsen, our potential therapy for Leber’s congenital 

amaurosis 10 (LCA10), a genetic disease causing blindness in childhood. 

“After two 
weeks he 
called his 
doctor  
and said  
HE WAS 
ABLE TO 
READ SIGNS” 

How did you feel when you got 
the results? 

learn. This makes it a modern study 

design that has been done before, 

“My feelings can be best described 

but it’s the first time it would be 

by talking about one of the patients, 

applied in an eye disease. With rare 

a 42-year old fella who had been 

diseases and small patient groups 

born with LCA10. Within years of 

it’s essential to be flexible in study 

birth he was already down to just 

design, and thankfully the regulators  

tunnel vision, but he was still able 

are enthusiastic and willing to listen.”

to read and function with glasses 

until his early twenties. By the time 

he entered the study, he could only 

distinguish light from dark. We then 

Seeing the success of sepofarsen, 
what’s your vision for ProQR 
in eye diseases? 

gave him the treatment, and when 

“We’re at a stage now where we 

he came back after four weeks he 

want to go both narrow and deep. 

was able to see bright lights in  

Now that we’ve shown that our RNA 

different colors. He was scheduled 

therapy had an effect on LCA10,  

to come in another month, but after 

we want to exploit that. We are do-

two weeks he called his doctor and 

ing an extensive search to make an  

said he was able to read signs.  

inventory of all genetic eye diseases,  

That was something he hadn’t done 

and select the ones that can be 

in twenty years. Then when he 

fixed using our toolbox of RNA  

came in for his second treatment 

therapies. This would give us  

he was reading letters on an eye 

a theoretical playing field. At the 

chart, for the first time since he was 

same time, we need to learn more 

a kid. That’s tremendous. It was 

about patients, because a lot of 

great for the company too, because 

people with vision problems don’t 

it’s really fortunate to see every-

know what genetic mutation is 

thing we work for come together  

causing their disease. This is why 

so early on in the process.

we work with foundations like 

Foundation Fighting Blindness who 

We did this study with 11 patients 

provide genetic testing to patients. 

and got very consistent responses, 

We could probably end up with a 

so we’re going to move to the next 

list of hundreds of diseases that we 

phase. We will start a seamless 

could potentially target, but then 

adaptive Phase 2/3 clinical study 

we have to prioritize.

that could be the last study we 

have to do before we get market 

The eye is a very good treatment 

approval. That it’s an adaptive 

area for RNA therapies. One major 

study means that we can adapt the 

issue with RNA therapies in general 

design as we go, based on what we 

is that it’s difficult to deliver it intact 

DAVID RODMAN, EXECUTIVE VICE PRESIDENT 
RESEARCH & DEVELOPMENT

TOWARDS 
A THERAPY 
FOR ONE 
PATIENT

PAG E IX
Magazi ne 
ANNUAL REPORT 2018

PAGE X
Ma ga zine 
ANNUAL REP ORT 2018

SEPOFARSEN (QR-110) FOR LEBER’S CONGENITAL AMAUROSIS

QR-1123 FOR P23H ADRP

LCA10

SEPOFARSEN

P23H ADRP

QR-1123

Lose sight in  
first years of life

p.Cys998X 
mutation affects  
~2,000 
patients in the 
Western world

Locally administered 
in the eye. Routine 
procedure

Anticipated 
infrequent dosing 
of 2 times a year

Goal: Restore 
vision/ prevent 
vision loss in 
patients with LCA10

Progressive reduction 
in night & peripheral 
vision. Blindness is 
frequent in  
mid-adulthood

~2,500 
patients with P23H 
adRP in United States

Locally administered 
in the eye. Routine 
procedure

Anticipated 
infrequent dosing 
of 2 times a year

Goal: Restore vision/
prevent vision loss 
in patients with 
P23H adRP

QR-421A FOR USHER SYNDROME

Develop hearing 
and vision loss in 
childhood and are 
completely blind  
by mid adulthood

USH2A exon 13 
mutations affect  
~16,000 
patients in the 
Western world

“We could 
end up  
with a list of 
HUNDREDS 
OF DISEASES 
that we 
could 
potentially 
target” 

to the relevant tissue, but in the  

Besides looking narrowly at LCA,  

a stage of tunnel vision and ending 

Instead of repairing the RNA like 

foreseeable future. Once we get 

eye this problem doesn’t exist.  

we are also taking a broader  

with complete blindness and 

we do for LCA and Usher, in adRP, 

the first programs I just described 

We can just inject it into the liquid 

perspective on retinal diseases.  

there is no therapy available to 

we are trying to selectively break 

done, and we’ve established the 

in the eye, actually a common pro-

For example, we have started  

them. This made me enthusiastic 

down the mutated version of the 

potential of RNA therapies in the 

cedure that is used for many drugs 

a clinical study in patients with 

about trying to make a difference. 

RNA that creates a toxic protein, 

eye, then the next step is to get to 

nowadays. The RNA therapy then 

vision loss due to Usher syndrome. 

Secondly, the disease progresses 

and not the healthy one.

what we call N=1 studies. To scale 

automatically spreads and enters 

This disease is the most common 

more slowly which means that 

down from rare diseases with  

all of the retinal cells, where it can 

cause of combined deafness and 

measuring the effect of the disease 

The work on adRP also made me 

a few thousand patients in the 

act. Another reason is that we  

blindness. This drug, QR-421a, uses 

and the therapy takes a long time. 

realize that with such subtle differ-

world to diseases with only hun-

have a very good and novel sys- 

exon skipping, a different mode of 

Ionis, the partner that discovered 

ences, we need to rethink what we 

dreds and, finally being able to 

tem to test potential therapies in.  

action to hopefully restore the vision 

the drug, had just followed a group 

measure in our clinical study too. 

treat versions of the disease that 

We can take stem cells from  

loss that these patients experience. 

of patients closely for two years to 

What actually matters to the  

are so rare that it affects perhaps 

a patient’s skin and grow these  

We are expecting to report the first 

map the progression. We can now 

patient? I think we should mea-

just one patient in the whole world. 

into ‘retina in a dish’, what we call 

results from this study in 2019.”

use that knowledge and save time 

sure, for example, if a therapy  

Obviously, this will blur the bound-

the optic-cup organoid model.  

This means we can test drugs in 

a very relevant setting, and the 

results suggest that it can predict 

what the drug can do in the real 

eye. A nice benefit is that these 

For another eye disease,  
autosomal dominant retinitis 
pigmentosa (adRP), you have 
licensed a program from Ionis. 
What’s the story there? 

in testing the drug. This helps the 

lets them run an errand quicker,  

ary between study and treatment, 

patients quicker. The third reason 

or allows them to drive a car.  

so we need to find a way to treat 

is that the disease is caused by 

We are also exploring the possibili-

individual patients with a new drug 

a very different type of mutation 

ties of virtual reality for this.

in a safe way while tracking their 

than we have thus far targeted. 

well-being. Once we have figured 

The type of RNA therapy is there-

Our aspiration for eye therapies 

that out, we will be able to help  

organoid models reduce the need 

“There are a few reasons really. 

fore different too, which gives us 

is that we want to start at least 

a lot more patients.” 

for animal testing too.

Firstly, adRP patients go through  

a chance to learn to work with it. 

one new program a year, for the 

PAG E XI
Magazi ne 
ANNUAL R EPORT 2018

PAGE XII
Ma ga zine 
ANNUAL REP ORT 2018

MAARTJE, LIVING WITH USHER SYNDROME

I WANT MY DAUGHTER 
TO BE ABLE TO DREAM, 
AND KEEP DREAMING

At a glance, it’s hard to tell Maartje de Kok – 41, married and mother of 

four – has Usher syndrome. Though she manages her life very well, it took 

her a long time to get to that point. “I have learned to be optimistic, but the 

sombreness is always there.”

“I have 
learned  
to be 
optimistic, 
but THE 
SOMBRENESS 
IS ALWAYS 
THERE”

during the day too. By the time  

syndrome, informing people about 

left. What can I do?’ and decided to 

I was 15 I was an avid netball player, 

life with Usher and raising funds for 

do a writing course and get a job. 

playing in the highest junior team. 

scientific research. “With our cam-

What I really rediscovered, I should 

But then during the summer I did 

paigns we stimulate young patients 

say, is my dreams. I dared to look 

a lot of reading in the sun with-

to ‘come out of the closet,’ and create  

into the future again.”

out sunglasses, and my vision got 

awareness” she says. “It’s important 

worse quite quickly. Because of my 

that we start understanding the 

A therapy is something she thinks 

experience and talent I was able to 

disease. For this reason the Usher 

about with some ambiguity.  

compensate for a while, but in the 

syndrome foundation supports  

“It’s scary to allow hope into my life, 

end I had to stop playing netball.  

the CRUSH study at Radboudumc  

because everything has always  

It was the same with going out;  

in Nijmegen, the Netherlands.  

been about things getting worse.  

I had trained myself to memorize 

The study and accompanying  

And I’ve heard promises from  

the route to the bathroom in a bar, 

database should uncover how  

doctors and researches before –  

for example. And when we cycled 

the disease progresses.” 

we were going to have a cure in  

home in the dark I would just focus 

10 years, and that was 20 years ago.  

Maartje lives in a brand new house 

Usher syndrome type 2, and that her 

on the markings on the bike path or 

A few years ago, Maartje spent two 

On the other hand, one of my daugh-

with her husband Peter and her kids 

field of vision is only six degrees,  

the lights in front of me.”

weeks hiking the pilgrim’s path to 

ters has Usher syndrome too. Jente 

Amber (11), the twins Ingmar and 

instead of 180. But it’s something 

Santiago de Compostela. During 

is eight years old, and she still has so 

Jente (8) and Kiki (6). She has a job as 

she has struggled with for a long 

As she got older, Maartje struggled 

those weeks she was challenged in 

many milestones ahead of her. With 

a social worker, helping parents who 

time. “I knew for sure when I was 19, 

with the new reality of losing both 

new ways. “A buddy accompanied 

Usher syndrome the clock is really 

have children with a visual handicap. 

although my ophthalmologist  

hearing and vision. “I used to have 

me to guide me through the hills. 

ticking; if there is a therapy before 

Together with Peter she manages 

had her suspicions when I was 15. 

terrible nightmares when I was  

Every day we slept in a different 

she’s 15, she might be able to keep 

her household quite effectively,  

I got hearing aids when I was 2.5 

adjusting to the idea of having 

place, which meant every day I had 

playing netball, which she enjoys  

and last summer she even gave back 

years old already, but only in hind-

Usher, and I’ve suffered two burn-

to learn new routes to the bath-

just like I did. If the therapy comes 

the guide dog that she had had for 

sight was it obvious that my eyesight 

outs and struggled with depression. 

room and exits. I managed very  

when she’s 18, netball and driving  

seven weeks. “It was the sweetest 

was deteriorating as well. I couldn’t 

Before I got the diagnosis I felt like  

well though, and applied all kinds  

a car might be out of the question 

dog, but it just felt like an extra child. 

play games in the dark during school 

I was ready to spread my wings and 

of tricks. It was a healing experience, 

but riding a bike or studying might 

Every time I finally had some time  

camp when I was 12, and I once fell 

make something of my life, but then 

and I found a lot of calm.”

not. And it goes on like that. That’s 

to sit down, I had to walk the dog.  

off the stage during a school musical 

it got taken away from me.” 

the time pressure that we’re under.  

It’s just better this way.” 

because somebody turned off the 

She also found a renewed ambition 

I know how Usher narrows life’s path 

To an outsider it might not be  

This night blindness slowly pro-

in social work and was involved in 

her education. “I thought to myself 

happen to her. I want her to be able 

immediately obvious that she has 

gressed until it affected my vision 

setting up a foundation for Usher 

‘Let’s say I have ten years of vision 

to dream, and keep dreaming.” 

lights before I reached backstage. 

Maartje managed to obtain a degree 

to work, to be productive and to use 

of choices, and I don’t want that to 

PAG E XIII
Magazi ne 
ANNUAL REPORT 2018

PAGE XIV
Ma ga zine 
ANNUAL REP ORT 2018

At ProQR, we want to attract talented scientists to take the field of RNA 

therapies to a higher level. For Seda Yilmaz-Elis, working in this field was 

the only thing she wanted after finishing her PhD. Now she works in our 

Black Box Innovation group, identifying the RNA therapies of tomorrow. 

“Working  
as a scientist  
at ProQR  
is basically 
MY DREAM 
JOB”

Seda came to the Netherlands  

for her PhD, which she did on  

the application of RNA therapies 

But what about other  
methods like gene editing  
and gene therapy?

(antisense oligonucleotides), at the 

“The advantage of an RNA therapy 

Leiden University Medical Center.  

is that we can take away the under- 

“After that I was in love with RNA 

lying cause of the disease in the 

therapies,” she recalls. “It was new, 

RNA, without permanently changing 

it was very innovative, and I still 

the patient’s genetic material,  

think it’s the therapy of the future.  

or DNA. This makes the treatment  

I also liked the translational applica-

reversible compared to gene editing  

bility of what I was working on.  

or gene therapy that make the 

You do something in the lab, and 

changes for life. Secondly, antisense 

you can see the results in the pa-

oligonucleotides do not usually 

tients later; from bench to bedside, 

require complex delivery systems, 

that is extremely motivating.  

like viral vectors for gene therapies. 

So when I completed my PhD,  

In most cases we can deliver it as 

I wanted to continue in this field 

a ‘naked molecule’ and it will reach 

and working as a scientist at ProQR 

the cells. This makes manufacturing 

was basically my dream job.”

and testing a lot easier.”

Fast forward a few years, and  

Seda made her dream come true – 

she started working at ProQR  

Your job at ProQR is in the ‘Black 
Box Innovation’ department. 
That sounds exciting, what is it? 

as an Associate Director and  

“Yes, it is super exciting! In a nut- 

project leader. 

Why do you like RNA therapies 
so much? 

shell, we aim to find new applica-

tions for ProQR’s toolbox of  

RNA Therapies. ProQR works on  

a number of diseases and therapeu-

“They can do the same job as  

tic areas like the eye. In the Black 

conventional drugs, but have 

Box we have a broader approach. 

broader applications. If the disease 

We try to find new diseases and 

is the result of an error in the DNA, 

even completely new therapeutic 

an RNA therapy can often fix it.  

areas that can benefit from RNA 

For example, they can be designed 

therapies. While we investigate,  

for a specific mutation, so it’s  

we always keep an eye out for rare  

potentially really personalized  

diseases, and target the ‘undruggable’.  

medicine. The therapy works with 

This is risky of course, but I like to 

short pieces of RNA, called anti-

root for the underdog.” 

sense oligonucleotides, it’s relative-

ly easy to manufacture once you 

How does that work? 

know what it needs to look like.”  

“First we try to identify a genetic  

disease, its gene and mutation(s). 

SEDA YILMAZ-ELIS, ASSOCIATE DIRECTOR
BLACK BOX INNOVATION

EXPLORING 
THE POTENTIAL 
OF RNA 
THERAPIES

PAGE XVI
Ma ga zine 
ANNUAL REP ORT 2018

PAGE XV
Magazi ne 
ANNUAL R EPORT 2018

“It is risky, 
but I like to 
root for the 
UNDERDOG”

ORGANOID MODELS 
FOR OLIGONUCLEOTIDE 
TESTING

We are looking for genetic defects 

done and positive, we hand the 

that can potentially be solved by 

best drug candidate over to the 

our RNA technology. We then  

clinical department to prepare it  

design an antisense oligonucle-

for clinical studies to test the  

otide, which is a short piece of RNA 

therapy in patients.”

ProQR uses organoid models to test the 

that might be able fix the issue. 

candidates for RNA therapy. An organoid 

The design is done ‘in silico’, which 

Do you do all this by yourself? 

model is a simplified organ grown in  

means on the computer, using 

“No, it’s always a team job and I’m 

a laboratory, using live human cells. 

databases and software. The design 

glad I have a great team with very 

They are made by extracting stem cells 

is only the start, the next step is 

dedicated and motivated people, 

from the skin of patients with the disease 

finding out which configuration of 

everybody has his/her own ex-

that the therapy is aimed at. These stem 

the design works best. This is done 

pertise and we also work in close 

cells can be triggered to become an 

by screening many similar designs 

collaboration with the other teams 

organ of choice – for example an eye. 

in cell cultures. The screening will 

within ProQR. Luckily we have  

When successful, these stem cells create 

give us a first impression of the 

a lot of expertise in-house but when 

a simplified eye, called an optic cup. 

therapy’s potential to improve the 

we need expertise from outside 

They can then be treated with the drug 

disease. It helps a lot that we can 

we also cooperate with the expert 

candidates, and the results can tell you 

do our own oligonucleotide man-

groups at the universities or with 

something about whether the therapy 

ufacturing in-house, which makes 

other companies depending on  

will work. The great advantage of this 

it quicker and more cost-effective 

the need.“

approach is that we can predict earlier 

to test hundreds of candidates in 

if a therapy can be successful, reducing 

order to find the most effective one.

cost and time. Another benefit is that 

After four years, is it still your 
dream job?

these models reduce the need for animal 

When we have selected the best 

“Yes! ProQR is a very nice organi- 

testing. ProQR currently has these 

candidates, we can go to the next 

zation to work in. We are a very 

models in place for skin and eyes. 

phase of testing. The models we 

dedicated group of people that put 

use for this are very sophisticated. 

a lot of effort into making patients’ 

We can for example take stem cells 

lives better. Within the company, 

from a patient with the disease we 

there is always room for improving  

want to target and grow the cells 

your skills, learning new things  

into mini organs called organoids. 

every day. The open culture with 

Our ophthalmology group lets 

very little hierarchy creates an  

those cells develop into simplified 

atmosphere where everybody  

eyes which allows us to predict 

can freely reach out to each other.  

if the therapy would work in the 

So when you have an idea it’s easy 

actual eye of that patient. We also 

to get people to listen, which is criti-

do initial tests to see if the therapy 

cal when you want to innovate.” 

Retinal organoid in development

is safe. Then, when all the tests are 

PAG E XVII
Magazi ne 
ANNUAL REPORT 2018

PAGE XVIII
Ma ga zine 
ANNUAL REP ORT 2018

“It DOESN’T 
STOP after 
granting an 
application 
for funds”

20 to 30 million dollars a year on 

In terms of partners, if it’s compa-

research and clinical trials. But that 

nies for the clinical development, 

doesn’t mean much if patients don’t 

we aim for parties that have a proven  

benefit from it. So we select care-

track record in safety, and that have 

fully and try to bring research into 

been able to bring something to  

blindness and retinal degeneration 

the clinic or even the market.  

further. To achieve this, we have an 

When it’s researchers we scrutinize 

application process for our grants, 

their science. We want the scientists 

and pull in outside subject experts 

we support to succeed. The group 

from all over the world to help us 

in Nijmegen, at Radboud University, 

select the best ones. We really want 

is a good example. They belong 

to pick groups and companies that 

to the best groups in the world, 

have a good chance of succeeding.

and have been an FFB center for 

years, receiving many investments 

As Chief Science Officer, it’s my  

from us. We actually funded their 

personal job to facilitate the ongoing  

research that is now being taken to 

research, to tackle upcoming 

the clinic by ProQR as sepofarsen, 

problems and to make sure we get 

which has gotten some very nice 

optimal results. Because it doesn’t 

results. Still preliminary, but looking 

stop after granting an application 

very promising. It’s important to 

for funds. We keep pushing for the 

get that proof of concept. Now we 

best preventions and cures, so that 

know that RNA therapies have the 

blind people benefit the most.  

potential to do what we believe it 

If we don’t get the most bang for 

will do in treating these diseases.”

our buck, it’s just a waste of funds.”

What kind of collaborations  
do you pursue? 

How about the developments 
with QR-421a for Usher  
syndrome?

“If you talk about research focus, 

“Well, it’s not as far as sepofarsen, 

we don’t believe in any particular 

but the collaboration with ProQR 

type of drug. The diseases causing 

so far has been fantastic. They’re 

blindness are as diverse as the 

transparent, forthcoming and I’m 

mutations that cause them, and 

impressed with their level of profes-

there is no silver bullet that will take 

sionalism. They really know how to 

care of it all. We therefore consider 

bring something to the clinic with  

gene therapy, gene editing, RNA 

a high regard for safety. We’re actu-

therapy – they all have potential. 

ally looking at additional projects to 

We also look at other diseases 

work on together.”

for inspiration. Take Parkinson’s 

disease or Alzheimer’s disease for 

example. These are brain-related, 

Do you feel like they mirror 
your high regard for patients? 

so they are based on neurobiology 

“Definitely. They really go the extra 

just like retinal diseases. The eye 

mile to ensure safety. My personal 

is the window to the brain and as 

yardstick is whether I would put  

such, understanding neurobiology 

my own children in a trial. If I can’t 

has implications for both brain and 

say that, then I don’t want to be in- 

retinal diseases.

volved. And with ProQR, the answer 

is ‘absolutely.”

STEPHEN ROSE, CHIEF RESEARCH OFFICER 
FOUNDATION FIGHTING BLINDNESS

WE PUSH FOR  
THE BEST RESEARCH, 
SO THAT PATIENTS 
BENEFIT THE MOST

The work we do at ProQR would not be possible without the help from 

patient organizations and foundations. One of our main supporters  

is the Foundation Fighting Blindness (FFB), who generously provided  

7.5 million dollars of funding for the clinical development of QR-421a,  

our drug candidate for Usher Syndrome. We checked in with Stephen Rose, 

Chief Science Officer at FFB, to talk about their perspective. 

The Foundation Fighting 
Blindness is a strong force  
in research funding when it  
comes to blindness. How does 
the foundation approach this? 

“With everything we do, the interest  

of patients is our top priority.  

We actually are the world’s leading 

private funder of inherited retinal 

disease research, spending 

PAG E XIX
Magazi ne 
ANNUAL REPORT 2018

“ProQR  
really goes 
the extra 
mile to 
ENSURE 
SAFETY”

You seem to have a strong  
personal connection with  
the foundation’s cause… 

Do you engage patients outside 
of studies?

“Yes, and it’s amazing how this 

“I do – I have two actually. I have  

has changed since the foundation 

a relative with a rare inherited form 

started in 1971. In the beginning, 

of retinal degeneration, and the 

we looked at blindness as needing 

more common age-related macular 

one cure. But now we know there 

degeneration runs in my family. 

are hundreds of different reasons, 

I participate in a clinical study 

mechanisms and varieties of retinal 

because of this, and I can tell you – 

degeneration. Obviously a big part 

some of those tests are not a lot of 

of this is genetics, so we provide 

fun! But it means that I have an ap-

programs for patients to test for 

preciation for the tests that patients 

known genes, but also to identify 

are required to complete in order 

new mutations. We still find new 

participate in our studies, and that 

ones every year.”

motivates me to really think about 

when tests are necessary and why. 

On a higher level, we often don’t 

How do you see the future for 
blind people? 

realize how much we use vision – 

“I’m cautiously optimistic. We’ve 

it’s underappreciated. Blind people 

been going at it for almost half  

have to learn everything by heart, 

a century, but the last five years the 

like the layout of their apartment or 

advances have been out of propor-

the way to the shop, and they rely 

tion, really great. It’s also hard to 

on others to help them by putting 

be specific. Will we find therapies 

stuff back in the same place every 

for everything? That’s a tall order. 

time. Can you imagine what hap- 

Will we be able to slow the progress 

pens if someone carelessly leaves 

of diseases? No doubt. Even in the 

the dishwasher door down?  

quest for the Holy Grail, which is  

We organize ‘dinners in the dark’ as  

regrowing the retina from stem 

a fundraiser, and it’s really powerful 

cells, we are making progress – 

for donors to have to learn that  

even though it has a way to go.  

the potatoes are at six o’ clock on 

In the end, it’s our job as a founda-

their plate, or to keep track of their  

tion to go out of business.” 

glass of water.” 

PAGE XX
Ma ga zine 
ANNUAL REP ORT 2018

We are with

124

ProQRians

WHAT ARE IRDS?

Nationalities

Average age

Gender

Inherited retinal diseases are a collection 

the most common cause of severe visual 

of rare retinal degenerations or retinal 

impairment or blindness in children and 

dystrophies, in which a genetic mutation 

people of working age. It is estimated that 

causes loss of function or death of the 

IRDs affect more than 2 million people 

light sensitive (photoreceptor) cells in 

worldwide. To date, around 300 genes 

the retina. Most common IRDs include 

have been identified that can cause IRDs 

retinitis pigmentosa, Usher syndrome, 

when mutated. For the vast majority of 

Leber’s congenital amaurosis and 

IRDs no therapy is currently available. 

Stargardt’s disease. They represent  

27

39yrs

44%

56%

PAG E XXI
Magazi ne 
ANNUAL REPORT 2018

PAGE XXII
Ma ga zine 
ANNUAL REP ORT 2018

“ProQR is 
PATIENT-
FOCUSED  
in a way  
I’ve never 
seen before”

JESSICA IBBITSON, VICE PRESIDENT 
OF CLINICAL AND DEVELOPMENT OPERATIONS

BREAKING 
BARRIERS TO 
BETTER THERAPIES

New therapies are thoroughly tested in clinical studies. This is necessary to ensure 

they are safe and efficacious, but for a company like ProQR, which aims to treat 

small groups of patients with rare diseases, innovating within the system is essential 

to removing the obstacles on the way to caring for patients. As one of our new faces 

of 2018, Jessica Ibbitson is trying to rethink the way we do clinical studies. 

Jessica joined ProQR at the 
beginning of 2018, after sev-
eral positions in the pharma 
industry. Her last job before 
the transition to Leiden was at 
Vertex Pharmaceuticals, where 
she worked on rare diseases. 
What drew you to ProQR? 

“I loved what I was doing, but when 

ProQR approached me I knew  

I couldn’t pass up on their offer.  

I was attracted by the fact that it 

was a smaller company, working  

on rare diseases too and patient- 

focused in a way I’ve never seen 

before. I saw an opportunity to 

be disruptive in terms of how we 

approach clinical studies.”

How do you see that now,  
after almost a year? What are 
your goals?

because a direct relationship with 

care. To guarantee this, we work 

a doctor who has only one point of 

side-by-side to explain our vision 

contact means that things move not 

in a way that makes them comfort-

“One way I’m trying to make ProQR 

only better, but quicker too.”

able. They really are our partners  

different is in our relationship with 

the hospitals that run our studies. 

We aim to have not just a closer 

Which barriers are you going  
to tackle next? 

in this, so we have to take this  

journey together.

relationship with them but a part-

“Well, in 2019 we’re starting to intro-

The other two ideas we are going 

nership. Even though we work with 

duce more digital technologies into 

to implement in the next year are 

clinical research organizations,  

our clinical trials. This, too, is about 

more patient-centric. We are going 

we recognize that it adds more 

eliminating barriers for participation 

to launch wearable and voice- 

links to a chain so we’ve decided 

in clinical research. For example, 

assistant devices. They are two 

to take on tasks in order to reduce 

we are trying to stimulate doctors 

sides to the same coin, because 

those links. By taking on more tasks 

to record more patient information 

both are ways to increase data, 

ourselves, we can limit the adminis-

digitally, on our eSource platform. 

reduce cost and reduce the burden 

trative burden of the doctor,  

This overcomes the inefficiency of 

on the patient and the hospital. 

so that they can spend more time 

double data entry, making it faster, 

The wearable is a bracelet that 

with their patients. But there is 

more accurate and less costly.  

tracks activity and sleep. This is 

more to this. A direct relationship 

But let’s not forget that it also gener-

very relevant for many patients 

also means that information travels 

ates insight more quickly, because it 

with eye-related diseases, because 

more freely, which means that it’s 

will instantly tell everyone involved 

when you’re visually impaired your 

much easier for us to understand 

how a treatment is going. And since 

day/night rhythm can also suffer. 

the daily practice of care, and what 

this also allows the doctor to spend 

Of course the bracelet measures 

the challenges and barriers are for 

more time with the patient, it cre-

these things in real time, so it gives 

hospitals, doctors and patients.  

ates more value too.”

us and the doctor much more data 

To make sure that they have no 

hesitations about joining a study, 

we need to eliminate as many of 

these barriers as possible.

How is the reception of this  
technology among doctors  
and hospitals?

to work with, and may uncover  

effects of the therapy that we 

would have otherwise missed.

“They are excited. We are aware 

The voice system is software that 

These barriers are not only about 

that the world of clinical practice 

you can install on your voice assistant  

value and information. As a small-

is very risk-averse. They have to 

that you can talk to and give  

er biotech company, we have to 

get used to new technology, and 

commands, like the Alexa device. 

think about efficiency as well – and 

be sure that changing their ways 

The potential it carries is different, 

often these goals go hand in hand, 

doesn’t affect the quality of their 

but multi-faceted. For example, 

PAG E XXIII
Magazi ne 
ANNUAL REPORT 2018

PAGE XXI V
Ma ga zine 
ANNUAL REP ORT 2018

it can take questionnaires from 

patients without the need for them 

What is your ideal future  
scenario?

to carry and store papers, which 

“We already went from a company 

is quicker and relieves patient 

that was maybe more traditional in 

burden. It could also remind them 

study design, to, in a very short time, 

to go to an appointment at the 

becoming really technology and 

hospital or to take their medication, 

digitally focused in terms of how we 

which helps compliance. And last 

execute studies. Looking ahead, the 

but not least, talking is much easier 

ultimate disruption for me would be 

for patients with eye-diseases than 

to have broken all barriers. One ideal 

reading and writing. Both the 

I have is ‘siteless clinical studies.’  

wearable and the voice assistant 

The need for a physician or hospital 

have the potential to reduce the 

will never go away completely,  

time needed for visits to the study 

but what if we could give patients 

hospital. This is significant for the 

a box containing their medication, 

patients that we work for; as some 

their wearable and other needs, and 

of them have to fly or cross borders 

they would be fully empowered. 

to visit the specialist.”

Then patients from all over the world 
could participate in our studies.” 

“One ideal 
I have is 
‘SITELESS 
CLINICAL 
STUDIES’”

THE THRU MY EYES APP

One digital innovation that Jessica  

is especially excited about is the  

Thru My Eyes app. It’s an app we 

developed that allows people to expe-

rience how patients with a certain eye-

disease are perceiving the world.  

Jessica: “The app is a simple but clever 

idea based around one question: how  

does a patient actually see? Using sliders, 

you can simulate certain characteristics of 

a disease, such as tunnel vision or visual 

acuity (sharpness of vision) on what the 

phone’s camera is seeing. We coupled it to 

medically relevant values, so it’s possible 

to set it to a certain individual’s diagnosis. 

This is a great tool for the patient’s loved 

ones to really get an idea of what he 

or she is going through. We’re eager to 

develop the app further, adding more 

disease simulations and functionalities.” 

Download the app via 

www.thrumyeyes.app

ANNUAL 
REPORT 
2018

Message from the CEO 

Key Figures 

Management Board 

Supervisory Board 

Management Board Report 

Supervisory Board Report 

Corporate Governance 

Risk Management 

Financial Statements 2018 

2 

3 

4 

5 

7 

35 

38 

48 

51 

 
 
 
 
Dear fellow shareholders, 

A first look at interim results in our Phase 1/2 clinical trial suggests that our lead product 

candidate, sepofarsen, improved vision in patients with Leber’s congenital amaurosis 10, 

the leading genetic cause of blindness in childhood. This is very encouraging and shows 

that our choice to focus on developing RNA therapies for rare genetic eye diseases is the 

right one. Only a small percentage of the 300 known genetic eye diseases currently have a 

treatment and we feel the responsibility to change that. Therefore, we are rapidly advancing 

our pipeline to have five programs for genetic eye disease in clinical trials in the next two 

years including QR-421a for Usher syndrome, the leading cause of combined deafness and 

blindness and QR-1123 for autosomal dominant retinitis pigmentosa.  

During our R&D Day we laid out ‘ProQR’s Vision 2023’ that details our strategy for the next 

five years. In this vision we have set ourselves the goal to build ProQR out into a multi-

product independent commercial company for inherited retinal diseases by the end of 

2023. In this period we aim to have our first two products approved and independently 

commercialized in the Western world, have three or more programs in late stage 

development, and have at least seven programs in earlier stages of development. This is a 

set of very ambitious goals that we aim for on our path to making a major impact to a large 

number of people around the world. 

But we also look beyond genetic eye diseases into other areas where our RNA repair 

technologies can make a difference for patients. We plan to advance several other 

preclinical programs in our broader rare disease pipeline of RNA therapies for patients in 

need. 

Daniel A. de Boer 

 
 
 
 
 
dsssds  

2018 

2017 

Result from continued operations (in € 1,000) 

Net revenue 

Other income 

Research and development costs 

General and administrative costs 

Operating result 

Net result 

Balance sheet information (in € 1,000) 

Non-current assets 

Current assets 

Total assets 

Total equity 

Non-current liabilities 

Current liabilities 

Cash flows (in € 1,000) 

Net cash used in operating activities 

Net cash used in investing activities 

Net cash generated by financing activities 

Ratio’s  

Current ratio 

Solvency (%) 

Figures per share 

Weighted average number of shares outstanding 

Basic and diluted earnings per share (in € ) 

Cash flow per share (in € ) 

Employees 

Average number of staff for the period 

-- 

5,761 

(29,514) 

(12,540) 

(36,293) 

(37,086) 

1,864 

108,367 

110,231 

92,685 

9,386 

8,160 

(28,493) 

(312) 

86,457 

-- 

1,495 

(31,153) 

(10,840) 

(40,498) 

(43,675) 

2,544 

50,559 

53,103 

39,325 

5,284 

8,494 

(34,951) 

(121) 

26,640 

13.3 

84.1 

6.0 

74.1 

34,052,520 

25,374,807 

(1.08) 

1.69 

(1.72) 

(0.33) 

127.7 

139.9 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
We have a two-tier board structure consisting of our Management Board (raad van bestuur) and a separate 

Supervisory Board (raad van commissarissen). The Management Board operates under the chairmanship of 

the Chief Executive Officer and shares responsibility for the deployment of ProQR’s strategy and policies, and 

the achievement of its objectives and results.  

Under Dutch Law, the Management Board has ultimate responsibility for the management and external 

reporting of the Company and is answerable to shareholders at the General Meeting of Shareholders. 

Pursuant to the two-tier corporate structure, the Management Board is accountable for its performance to a 

separate and independent Supervisory Board. 

The following table sets out information with respect to our Management Board member, his respective age 

and his position at the Company as of the date of this annual report. 

Name 

Gender 

Date of Birth 

Position 

Date of  
Appointment 

Term 
expires 

Daniel de Boer 

Male 

April 12, 1983 

Chief Executive Officer 

February 21, 2012 

2022 

The following sets forth biographical information regarding our Management Board members. 

Daniel de Boer is our founding Chief Executive Officer since our incorporation in 2012. Daniel is a serial-

entrepreneur and passionate advocate for rare disease patients. He assembled a group of successful biotech 

executives as co-founders and built a team of a 150 experienced scientists and drug developers, devoted to 

creating RNA therapies for patients in need. Under Daniel’s leadership ProQR initiated clinical trials in 

multiple development programs for rare diseases, and raised over $ 300M in funding, including an IPO on 

Nasdaq. Daniel is responsible for the overall strategy and general business in the company. Before founding 

ProQR, Daniel was founder and Chief Executive Officer of RNA Systems, PC Basic and Running IT, companies 

he led through phases of growth, developing and launching several products in multiple European countries. 

Daniel was also a co-founder of Amylon Therapeutics, a company developing therapies for genetic brain 

diseases. In 2018 Daniel was named "Emerging Entrepreneur of the Year" by EY. 

During 2018 René Beukema was our Chief Corporate Development Officer and General Counsel. Mr. Beukema 

joined us in September 2013 and is a seasoned in-house corporate lawyer in the Dutch biotechnology arena. 

Prior to joining us, Mr. Beukema served as General Counsel and Corporate Secretary of Crucell N.V. for twelve 

years, following his experience as a Senior Legal Counsel at GE Capital / TIP Europe and Legal Counsel at TNT 

Express Worldwide. Mr. Beukema was also a venture partner of Aescap Venture, a life sciences venture 

capital firm. Mr. Beukema is co-founder and advisor of Mytomorrows N.V., a Dutch life sciences company. He 

holds a post-doctoral degree in corporate law from the University of Nijmegen in co-operation with the Dutch 

Association of In-house Counsel (Nederlands Genootschap van Bedrijfsjuristen) and a Master’s degree in 

Dutch law from the University of Amsterdam. Mr. Beukema left the Company January 1, 2019. 

 
 
 
 
 
 
 
 
 
 
 
The Supervisory Board supervises the policies of the Management Board and the general course of affairs of 

ProQR and advises the Management Board thereon. The Supervisory Board, in the two-tier corporate 

structure under Dutch law, is a separate and independent corporate body. 

The following table sets forth information with respect to each of our Supervisory Board members and their 

respective dates of birth. The terms of office of all our Supervisory Board members expire according to a 

rotation schedule drawn up by our Supervisory Board. 

Our Supervisory Board is currently composed of the following members, all of whom are independent under 

applicable NASDAQ standards and all of whom, with the exception of Mr. Dinko Valerio and Mr. Antoine 

Papiernik are independent under the Dutch Corporate Governance Code (DCGC): 

Name 

Gender  Nationality 

Date of Birth 

Position 

Date of Appointment 

Term expires 

Dinko Valerio 

  Male 

Alison Lawton 

Female 

Antoine Papiernik   Male 

James Shannon 

Paul Baart 

Male 

Male 

NL 

US 

FR 

GB 

NL 

August 3, 1956  Chairman 

January 1, 2014 

September 26, 1961  Member 

September 17, 2014 

July 21, 1966  Member 

January 1, 2014 

June 5, 1956  Member 

November 9, 1950  Member 

June 21, 2016 

June 10, 2015 

2020 

2022 

2021 

2020 

2019 

The following sets forth biographical information regarding our Supervisory Board members.  

Dinko Valerio is one of our founders and currently serves as the chairman of our Supervisory Board. Mr. 

Valerio has served on our supervisory board since January 2014. Mr. Valerio is a scientist and an experienced 

biotech entrepreneur with experience in both public and private companies as CEO and board member. Mr. 

Valerio is founder and former CEO of Crucell N.V., a Dutch biotech company, and founder and former general 

partner of Aescap Venture, a life sciences venture capital firm. In 1999, Mr. Valerio was one of the founders of 

Galapagos Genomics N.V., a spinout from Crucell N.V. which develops novel mode of action medicines. In 

2017 Mr Valerio became a boardmember of Amylon Therapeutics B.V., a 80% owned affiliate of ProQR 

Therapeutics N.V. Adding to his corporate experience, Mr. Valerio is a professor in the field of gene therapy of 

the hematopoietic system at the University of Leiden. He received his Master of Science degree in Biology 

from the University of Amsterdam in 1982 and completed his Ph.D. in Molecular Genetics with Honors at the 

University of Leiden in 1986. Mr. Valerio also was a visiting scientific specialist at Genentech Inc., San 

Francisco in 1985 and a postdoctoral fellow at the Salk Institute, San Diego from 1986 to 1987. He is an 

author on more than 100 articles in peer-reviewed journals and an inventor on 11 patent-families. 

Alison Lawton has served on our supervisory board since September 2014. Ms. Lawton is currently Chief 

Executive Officer, President and Director of Kaleido Biosciences where she was previously President and 

Chief Operating Officer since Dec 2017. Previously, Ms. Lawton was Chief Operating Officer at Aura 

Biosciences, Inc, from 2015 to 2017, Ms. Lawton served as Chief Operating Officer at OvaScience Inc., a life 

sciences company, from January 2013 to January 2014. In addition, from 2014 to 2017, Ms. Lawton served as 

a biotech consultant for various companies, including as Chief Operating Officer consultant at X4 

Pharmaceuticals. Ms. Lawton worked at various positions of increasing responsibility at Genzyme 

Corporation, or Genzyme, and subsequently at Sanofi-Aventis, following its 2011 acquisition of Genzyme, 

each a global biopharmaceutical company. Ms. Lawton served as head of Genzyme Biosurgery, where she 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
was responsible for Genzyme’s global orthopedics, surgical and cell therapy and regenerative medicine 

businesses. Prior to that, Ms. Lawton oversaw Global Market Access at Genzyme, which included Regulatory 

Affairs, Global Health Outcomes and Strategic Pricing, Global Public Policy, and Global Product Safety & Risk 

Management. Before joining Genzyme, Ms. Lawton worked for seven years in the United Kingdom at Parke-

Davis, a pharmaceutical company. Ms. Lawton serves on the board of directors of Verastem, Inc., a public 

biopharmaceutical company. She also served on the board of directors of Cubist Pharmaceuticals for three 

years until its acquisition by Merck &Co., Inc. in 2015. She currently sits on the Scientific Advisory Board for 

the Massachusetts Life Science Center. She is past President and Chair of the Board of Regulatory Affairs 

Professional Society and past FDA Advisory Committee member for Cell and Gene Therapy Committee. She 

earned her BSc in Pharmacology, with honors, from King’s College London. 

Antoine Papiernik has served on our supervisory board since January 2014. Mr. Papiernik is managing partner 

at Sofinnova Partners, which he joined in 1997, and was appointed chairman in 2017. Mr. Papiernik has been 

an initial investor and active board member in public companies like Actelion, Addex, Auris Medical, Orexo, 

NovusPharma (then sold to CTI), Movetis (then sold to Shire), Mainstay, Pixium and Stentys, which went public 

respectively on the Zurich Stock Exchange, the NASDAQ Global Market, the Stockholm Stock Exchange, the 

Milan Nuovo Mercato, the Belgium Stock Exchange, the Dublin Stock Exchange and EuroNext Paris, in 

Cotherix (initially NASDAQ listed, then sold to Actelion), Core Valve (sold to Medtronic), Fovea (sold to Sanofi 

Aventis)m Ethical Oncology Science (EOS, sold to ClovisOncology) and Recor Medical (sold to Otsuka). Mr. 

Papiernik is also a board member of private companies MedDay Pharmaceuticals, MD Start, Shockwave 

Medical, Reflexion Medical, Gecko Biomedical, SafeHeal, Highlife and Rgenix. Mr. Papiernik has an MBA 

degree from the Wharton School of Business, University of Pennsylvania. 

James Shannon, MD has served on our Supervisory Board since June 2016. Mr. Shannon has had an extensive 

career in drug development and pharma. From 2012 until his retirement in 2015, Mr. Shannon was Chief 

Medical Officer at GlaxoSmithKline. Prior to that he was Global Head of Pharma Development at Novartis and 

Senior Vice-President, Clinical Development at Sterling Winthrop Pharmaceuticals. He held board positions at 

companies including Biotie, Circassia, Crucell, Endocyte, MannKind and Cerimon Pharmaceuticals. In 2017 he 

joined the board of directors of Horizon Pharma. He received his undergraduate and postgraduate degrees 

at Queen’s University of Belfast and is a Member of the Royal College of Physicians (UK). Mr. Shannon 

currently holds board positions at Mannkind Corp (USA), myTomonows (NL), Horizon Pharma (Ire) and 

lmmodulon (UK). 

Paul Baart has served on our supervisory board since June 2015. Mr. Baart made his career in public 

accounting in both the Netherlands and the USA. At PwC the Netherlands he served on the management 

board and the supervisory board. He was also a member of the global board of PwC International. He has 

served many large (listed) and international clients in various industries. He held professional qualifications 

both in the Netherlands and in the USA. He was chairman of Royal NIVRA, the Dutch Institute of Registered 

Accountants (now NBA), member of the Dutch Council on Annual Reporting (RJ) and supervisory board 

member of Nyenrode Business University. Present roles include outside member Enterprise Chamber 

Amsterdam Court of Appeal (Ondernemingskamer) and chairman Supervisory Board Grant Thornton the 

Netherlands. He studied business economics at the Vrije Universiteit in Amsterdam, where he also passed the 

Registeraccountants exam. 

 
 
 
 
The Company 

ProQR Therapeutics N.V., or “ProQR” or the “Company”, is dedicated to changing lives through the creation of 

transformative RNA medicines for the treatment of severe genetic rare diseases (sometimes called orphan 

diseases) such as Leber’s congenital amaurosis 10, Usher syndrome type 2 and autosomal dominant retinitis 

pigmentosa. Based on our unique proprietary RNA platform technologies, we are growing our pipeline with 

patients and loved ones in mind. 

ProQR was founded in February 2012 by Daniel de Boer, Gerard Platenburg, the late Henri Termeer and 

Dinko Valerio. Mr. de Boer is a passionate and driven entrepreneur and an advocate for patients with severe 

genetic diseases. He has assembled an experienced team of successful biotech executives as co-founders, 

management team members and early investors. The team has extensive experience in the discovery and 

development of products in multiple therapeutic areas. As of December 31, 2018, we had raised € 251 million 

in gross proceeds from our public offerings of shares on the NASDAQ Global Market and private placements 

of equity securities. In addition, we have received grants, loans and other funding from patient organizations 

and government institutions supporting our programs, including from Foundation Fighting Blindness, 

Epidermolysis Bullosa Research Partnership, Epidermolysis Bullosa Medical Research and the Dutch 

government under the innovation credit program. ProQR headquarters are located in Leiden, the 

Netherlands.  

Our legal name is ProQR Therapeutics N.V. and we were incorporated in the Netherlands, on February 21, 

2012. We reorganized from a private company with limited liability to a public company with limited liability 

on September 23, 2014. Our company has its statutory seat in Leiden, the Netherlands. The address of its 

headquarters and registered office is Zernikedreef 9, 2333 CK Leiden, the Netherlands, telephone number 

+31 88 166 7000. The name and address of our agent for service in the United States is CT Corporation 

System, 111 Eighth Avenue, New York, NY 10011. We also rent offices in the United States in Cambridge, MA. 

Since September 18, 2014, our ordinary shares have been listed on the NASDAQ Global Market under the 

ticker symbol PRQR. 

Operations 

We are an innovative biopharmaceutical company engaged in the discovery and development of RNA-based 

therapeutics for the treatment of severe genetic rare diseases. Utilizing our RNA platform, we are building a 

pipeline of therapeutics for patients in need. Our drug development programs are based on single-stranded 

RNA oligonucleotides that are chemically modified to enhance stability and cellular uptake, and intended to 

correct the underlying cause of the disease through repairing the genetic defect in the RNA. While all our 

compounds are RNA-based, a variety of mechanisms of actions may be used depending on the type of 

mutation causing the disease. We believe that this targeted approach offers several advantages compared to 

other therapeutic approaches in the treatment of the rare genetic diseases we target.  

Our current pipeline consists of programs in ophthalmology and dermatology. In ophthalmology, we have a 

deep and broad pipeline with sepofarsen (formerly named QR-110) for Leber’s congenital amaurosis 10, or 

LCA10 as our most advanced program. We are currently planning to start a potential pivotal Phase 2/3 clinical 

trial with sepofarsen during the first half of 2019 while completing a Phase 1/2 clinical trial that reported a 

rapid and sustained improvement in vision during an interim analysis. In dermatology, our most advanced 

program, QR-313, targets dystrophic epidermolysis bullosa, or DEB, a severe genetic blistering skin disease. 

We recently announced that post a planned interim analysis from our ongoing blinded Phase 1/2 clinical trial 

 
 
 
 
and a strategic review of our portfolio, further development of this program will be conducted by Wings 

Therapeutics. 

Beyond our clinical portfolio, we have discovered and developed a novel proprietary RNA editing platform 

technology called Axiomer®. Axiomer’s editing oligonucleotides, or EONs, are designed to recruit 

endogenous Adenosine Deaminases Acting on RNA, or ADAR, enzymes to make single nucleotide changes in 

the RNA in a highly specific and targeted manner at a desired location. We believe our Axiomer platform may 

be applicable to more than 20,000 disease-causing mutations. 

We continue to assess our development and commercialization plans for our product candidates and intend 

to evaluate opportunities for beneficial collaborations or partnerships for these programs. In addition, using 

our discovery engine that is designed to generate a deep and broad pipeline of product candidates, we seek 

to enter into strategic partnerships for programs that we believe will benefit from such a partnership, and 

advance other selected programs independently to commercialization. 

Our RNA Repair Technologies  

DNA contains genes that provide the instructions for the body to make all the functional building blocks of 

the cells, such as proteins. To get from DNA to protein, the cell first copies the information in the DNA into 

RNA during a process called transcription. The RNA then acts as the blueprint for making proteins during a 

process called translation. Genetic diseases are caused by mutations, or defects, in the DNA. These mutations 

are copied into the RNA blueprint, which means the resulting protein is also made incorrectly. The result is a 

missing, defective or toxic protein that prevents the cell from carrying out its normal function causing the 

disease.  

We have gathered a toolbox of novel RNA repair technologies with which we believe we can use to target 

genetic diseases that are currently untreatable or have limited effective treatment options. Repairing RNA can 

take away the underlying genetic cause of the disease without having to make permanent changes to a 

patient’s DNA. Our current molecules are all single-stranded RNA-based oligonucleotides that are chemically 

modified so that no vector or envelope is needed for delivery.  

The toolbox of technologies range from splice correction in which we aim to restore normal messenger RNA 

and protein, exon skipping in which we aim to exclude the mutated part of the RNA and restore protein 

function to a gapmer technology that could prevent the formation of a toxic mutated protein. We believe our 

RNA repair approach has several advantages over DNA approaches such as gene therapy and gene editing. 

 
 
 
 
 
 
These RNA repair approaches could allow us to develop novel RNA therapies for genetic diseases and make a 

meaningful impact on the lives of patients suffering from them. 

Research and development pipeline 

Sepofarsen for Leber’s Congenital Amaurosis 10 

Leber’s Congenital Amaurosis (LCA) is the most common genetic cause of blindness in childhood of which 

LCA10 is one of most severe forms. People with LCA10 typically become blind within the first few years of life 

and there are currently no approved therapies. The most common mutation is the p.Cys998X (also known as 

c.2991+1655A>G) in the CEP290 gene. Although prevalence rates vary, based on our estimations, we believe 

this mutation occurs in approximately 2,000 patients in the Western world. 

We are developing sepofarsen (formerly named QR-110) for patients who have LCA10 due to the p.Cys998X 

mutation. Sepofarsen aims to repair the underlying cause in the RNA by splice correction. This RNA splice 

correction is designed to result in the production of the normal, or wild type, CEP290 protein stopping or 

potentially reversing the disease. Sepofarsen is designed to be administered through intravitreal injections in 

the eye. 

A Phase 1/2 clinical trial is ongoing in adults and children with LCA10 due to the p.Cys998X mutation. In 

September 2018, we reported an interim analysis confirmed clinical proof-of-concept as shown by a rapid 

and sustained improvement in vision in the majority of patients. In January 2019, we reached agreement with 

the U.S. Food and Drug Administration (FDA) on the design of a proposed Phase 2/3 clinical trial for 

sepofarsen. This planned Phase 2/3 clinical trial, named ILLUMINATE, is expected to start during the first half 

of 2019 and could serve as the sole registration trial for the program. Beyond sepofarsen, we have an 

additional discovery-stage program, QRX-136, for another mutation in CEP290. 

Sepofarsen has been granted orphan drug designation by the FDA and European Commission and received 

fast track designation by the U.S. FDA 

QR-421a and QR-411 for Usher syndrome type 2 

Usher syndrome is the leading cause of combined hearing loss and blindness. To date, there are no therapies 

approved or product candidates in clinical development that treat the vision loss associated with the disease. 

Usher syndrome type 2 is one of the most common forms of Usher syndrome and is caused by mutations in 

the USH2A gene which encodes a protein called usherin.  

We are developing QR-421a for USH2A exon 13 mutations and QR-411 for the USH2A PE40 mutation. In the 

Western world, approximately 16,000 patients have vision loss due to mutations in exon 13 of the USH2A 

gene and approximately 1,000 patients are affected by the PE40 mutation. Both product candidates are RNA 

 
 
 
 
 
therapies intended to be administered by intravitreal injections and that aim to restore functional usherin 

protein in the eye to restore vision. Beyond QR-421a and QR-411 we have an additional discovery-stage 

program, QRX-461, for another mutation in USH2A. 

Clinical development of QR-421a has begun and we plan to announce data from the ongoing Phase 1/2 safety 

and efficacy trial, named STELLAR, in mid-2019. QR-411 is currently in preclinical testing. 

QR-421a and QR-411 have received orphan drug designation from the FDA and EMA. QR-421a was also 

granted fast track designation by the FDA. 

QR-1123 for autosomal dominant retinitis pigmentosa 

Autosomal-dominant retinitis pigmentosa (adRP) is characterized by progressive loss of vision. Symptoms 

typically start in early teenage years and include night blindness and reduction of the peripheral vision 

leading to tunnel vision. Eventually patients lose their central vision and become completely blind during 

adulthood. In the United States, the P23H mutation in the RHO gene is the most common mutation causing 

adRP and affects approximately 2,500 patients. 

We are developing QR-1123 that was discovered by Ionis Pharmaceuticals and in-licensed by us in October 

2018. QR-1123 is designed for the treatment of P23H adRP by suppressing the formation of the toxic mutant 

protein. By mutant-specific knockdown, QR-1123 selectively targets the mutant P23H RNA for destruction by 

RNase H1 cleavage without affecting the wild-type RNA. By reducing the mutant RNA, the resulting toxicity-

induced loss of photoreceptors and subsequent loss of vision can potentially be stopped or reversed. 

Currently, the QR-1123 program is undergoing the final preparation stages for IND submission. We plan to 

advance the QR-1123 program into a Phase 1/2 clinical trial during 2019. 

QR-313 for Dystrophic Epidermolysis Bullosa (DEB) 

Dystrophic epidermolysis bullosa (DEB) is a devastating skin disease that results in severe blistering and 

poorly healing wounds over the entire body, including mucosal membranes. Patients with the recessive form 

of DEB (RDEB) have a limited life expectancy and low quality of life. There is currently no treatment available 

for DEB besides intensive and costly palliative care. DEB is caused by mutations in the COL7A1 gene which 

leads to an absence of functional collagen type VII (C7) protein which is essential for the formation of 

anchoring fibrils that link the outer layers of skin, the epidermis, to the dermis.  

We are developing QR-313 for exon 73 mutations in the COL7A1 gene. Approximately 2,000 DEB patients in 

the Western world have a mutation in this part of the gene. QR-313 is designed to be topically applied to a 

patient’s wounds as a hydrogel and aims to restore functional C7 protein that is able to form anchoring fibrils 

to improve the strength of the skin. Beyond QR-313, we have a pipeline of discovery-stage programs for other 

mutations that cause DEB. 

Subsequent to a planned interim analysis and strategic review, management has elected to transfer conduct 

and completion of the ongoing Phase 1/2 study to Wings Therapeutics. The ongoing Phase 1/2 trial in patients 

with DEB due to a mutation in exon 73 will remain blinded and continues to enroll patients. ProQR will work 

closely with Wings Therapeutics and EBRP to support its efforts to advance QR-313 for patients with DEB. 

QR-313 has received orphan drug designation from the FDA and EMA. 

Eluforsen for Cystic Fibrosis (CF) 

Cystic fibrosis (CF) causes viscous mucus to accumulate in vital organs disrupting several processes in the 

body. Pancreatic enzymes are blocked from entering the intestines and the thick layer of mucus in the lungs 

 
 
 
is a great environment for destructive bacteria. The thick mucus makes it hard to clear the lungs from these 

bacteria and results in regular infections and inflammation. This process injures the lungs and leads to 

frequent hospitalizations and lung failure. 

We are developing eluforsen for the most common mutation causing CF, the F508del mutation in the CFTR 

gene, affecting approximately 85% of all CF patients. Two global clinical trials for eluforsen in people with CF 

have been completed. Study 001, a Phase 1b safety and tolerability clinical trial in 70 CF patients and Study 

002, a proof of concept clinical trial in 18 CF patients. In both clinical trials eluforsen was observed to be safe 

and well-tolerated and both trials showed encouraging signals that eluforsen has the potential to be a 

meaningful therapy for people with CF that have two copies of the F508del mutation (homozygotes). 

Eluforsen has received orphan drug designation from the FDA and EMA. Eluforsen was also granted fast track 

designation by the FDA. 

Axiomer® RNA Editing Technology  

The Axiomer® platform is a novel, proprietary RNA editing technology invented at ProQR. The technology is 

based on editing oligonucleotides, or EONs, designed to recruit ADAR enzymes (Adenosine Deaminases 

Acting on RNA) to make single nucleotide changes in the RNA in a highly specific and targeted manner at a 

desired location. The approach, for which ProQR is pursuing patent protection, allows the recruitment of 

endogenous ADARs by using EONs as the sole drug modality, doing away with the need for overexpression of 

(artificial) ADAR proteins, guide RNAs or other large, complex components. 

Recruitment of endogenous RNA-editing enzymes by EONs represents a significant therapeutic opportunity 

for a new type of drugs that can treat genetic diseases by reversing the underlying mutations. ADARs are 

present in most human cells and naturally make adenosine-to-inosine (A-to-I) changes in RNA. Since an 

inosine is interpreted by the cell as a guanosine, an EON-mediated, targeted editing reaction has the potential 

to effectively modify any chosen adenosine (A) in any RNA to a guanosine (G). This can either restore the 

original sequence, or bring about an intended de novo A to G change, in order to treat genetic disease. 

Current estimations point to over 20,000 G to A mutations in the human population that cause disease. 

In vitro and in vivo work indicates that the EONs are generally applicable for the correction of mRNA G-to-A 

mutations. Together with the leading academic experts in RNA editing, we continue to advance our Axiomer 
RNA Editing technology to develop therapies for genetic diseases.  

Early stage pipeline 

Beyond the programs mentioned above we have additional early stage programs in our pipeline targeting 

genetic diseases with profound unmet medical need.  

QR-504 for Fuchs endothelial corneal dystrophy 

Fuchs’ endothelial corneal dystrophy 3 (FECD3) is a common, autosomal dominant, degenerative condition of 

the eye. With age the endothelial cells are lost, ultimately leading to progressive corneal clouding, reduced 

vision and painful epithelial bullae. There are currently no treatment options other than corneal 

(endothelium) transplantation for patients with advanced disease. The availability of donors, risk of rejection, 

and the inherent risk of an invasive procedure are some of the limitations of this procedure. FECD3 is caused 

by a trinucleotide CTG repeat expansion in the TCF4 gene. It is estimated that FECD affects more than 4% of 

individuals over the age 40 in the U.S., and similar prevalence is noted for other global regions. The mutated 

TCF4 mRNAs accumulate as nuclear RNA foci and globally disrupt mRNA splicing in the corneal endothelial 

cells. QR-504 targets the mutated mRNA with the aim to reduce the accumulation and splicing disruption. 

QR-504 is currently in discovery stage and we intend to commence IND-enabling studies.  

 
 
 
 
QR-1011 for Stargardt’s disease 

Stargardt’s disease is the most common inherited macular dystrophy causing progressive loss of central 

vision. Most patients with Stargardt’s disease will progress to legal blindness or worse as they age. Currently, 

there is no treatment available. It is associated with mutations in the ABCA4 gene resulting in the loss of 

photoreceptor cells in the retina. The c.5461-10T>C mutation affects about 7,000 patients in the Western 

world and leads to aberrant splicing of ABCA4 mRNA. QR-1011 aims to restore normal splicing leading to the 

production of wild type mRNA and protein thereby stopping or potentially reversing the disease. QR-1011 is 

currently in the advanced lead optimization phase. 

QRX-704 for Huntington’s Disease  

Huntington’s disease (HD) is an inherited progressive neurodegenerative disease, and one of the most 

common genetic disorders. Symptoms include involuntary movements, incoordination, impaired speech, 

cognitive decline and depression. Patients with HD have a shortened life expectancy and there is currently no 

disease-modifying treatment available. The disease is caused by an expanded repeat of CAG nucleotides in 

the HTT gene, resulting in a mutated huntingtin protein that is cleaved into toxic fragments, which 

accumulate in nerve cells. QRX-704 is designed to modify HTT mRNA to prevent the formation of the toxic 

fragments, while the huntingtin protein remains functional. QRX-704 is currently in discovery stage.  

Our Strategy  

We are dedicated to improving the lives of patients and their loved ones through the development of RNA 

therapies for severe genetic rare diseases. We believe the strategy as outlined below enables us to build a 

sustainable independent business which creates value for all stakeholders involved. Key elements of our 

strategy include:  

  Develop drugs for patients in need. Through our patient-centric approach we work to develop best-in-

class therapies and to advance the understanding of conditions that we target. As RNA therapies have 

become an established modality, we are translating new applications in a pipeline of products for 

patients suffering from rare diseases.  

 

Rapidly advance our ophthalmology platform. The initial results of sepofarsen in restoring vision as 

observed during the interim analysis of the Phase 1/2 trial have built confidence in the potential 

opportunity for RNA therapies in treating genetic eye diseases. Therefore, we plan to rapidly advance our 

programs in ophthalmology for a range of genetic eye diseases for which there are no or limited 

treatment options. As part of our five-year plan known as our “ProQR Vision 2023 strategy”, by 2023, we 

aim to obtain marketing approvals for the first two products in our ophthalmology pipeline, and build a 

deep pipeline of ten or more programs beyond those two products, of which we expect three to be in late 

stage development. 

 

Commercialize portfolio of ophthalmic medicines independently. We plan to commercialize our portfolio 

of medicines for inherited retinal diseases (IRDs) independently in North America and Europe, and seek 

partners for other geographic areas. While building the commercial infrastructure for an expected 

commercial launch of sepofarsen in 2021, we expect this same infrastructure to serve patients with other 

IRDs like Usher syndrome or Stargardt’s disease as IRD patients are typically seen by one of the 30 IRD 

hub centers. 

 

Leverage our pipeline through strategic consideration of out-licensing, spinouts or collaborative 

partnerships. We plan to continue to advance the programs and technologies in our discovery pipeline 

beyond ophthalmology and selectively engage with partners for development and commercialization of 

programs and products that we do not intend to independently develop. 

 

Expand our Axiomer RNA-editing platform into select therapeutic areas. Our novel and proprietary RNA 

editing platform technology, Axiomer, is a new way to use oligonucleotides to edit single nucleotides in 

the RNA. We believe our Axiomer technology may be applicable to more than 20,000 disease-causing 

mutations. In 2019 and beyond, we plan to build out Axiomer in select therapeutic areas and continue to 

 
 
 
validate and create value for the platform through pursuing licensing, partnering and other strategic 

relationships.   

Patient Centric Approach 

ProQR aims to develop best-in-class therapies as well as to improve patient care through awareness, 

education, and advancing the understanding of conditions that we target. In order to achieve this goal, ProQR 

strives to integrate the patient voice into our decision-making throughout the drug development process as 

we believe that a patient-centric strategy is crucial to our success. Therefore, our Patient and Medical 

Community Engagement (PMCE) team actively collaborates with and listens to the communities we serve to 

ensure that the patient voice is represented internally. 

Sepofarsen for Leber’s Congenital Amaurosis 10 (LCA10) 

LCA background 

Leber’s Congenital Amaurosis (LCA) is the most common genetic cause of blindness in childhood. The 

p.Cys998X mutation (also known as c.2991+1655A>G) in the CEP290 (Centrosomal protein of 290 kDa) gene is 

the most prevalent mutation which generally accounts for the most severe disease phenotype (LCA10). This 

mutation leads to significant decrease in CEP290 protein within the photoreceptor cells in the retina. Patients 

affected by this mutation typically lose sight in the first years of life. Clinical features of LCA10 include loss of 

vision, involuntary eye movement or nystagmus, abnormalities of pupil reactions and no detectable 

photoreceptor electrical signals on electroretinography (ERG).  

Representation of the p.Cys998X  
mutation causing LCA10 

LCA genetics 

More than 20 genes have 

been associated with the 

genetic defect that causes 

LCA. The most common 

mutation is the p.Cys998X 

in the CEP290 gene 

causing LCA10. The 

p.Cys998X mutation is a 

single nucleotide 

substitution in the CEP290 

gene that creates a new 

splice site, also called a 

cryptic splice site, 

between exon 26 and 27. 

During the splicing of the 

pre-mRNA this causes a 

part of the intron, or 

pseudoexon, to be 

included in the mRNA. 

The pseudoexon contains 

a premature stop codon 

thus the mRNA is not translated into the full length CEP290 protein. CEP290 protein is involved in the 

formation and stability of the connecting cilium in photoreceptor cells, which facilitates the transport of 

proteins from the inner segment to the outer segment of the cell. When CEP290 is absent, there is a 

disturbance in normal protein transport to the outer segments of the photoreceptor cell which provokes the 

shortening of the outer segment and its inability to perform its light transducing function.  

 
 
 
 
 
LCA Prevalence and Diagnosis  

LCA affects about 15,000 patients in the Western world. Although diagnosis rates vary, our estimations 

indicate the most common p.Cys998X mutation occurs in approximately 2,000 patients in the Western world.  

Patients are initially diagnosed through the presence of clinical symptoms. Nystagmus, rapid involuntary 

movements of the eyes, tends to be the first symptom visible as well as oculo-digital signs comprising eye 

poking, pressing, and rubbing. Vision impairment or blindness becomes obvious as age increases. After an 

ophthalmological examination, LCA is diagnosed. A genetic screening including all known mutations causing 

LCA is performed to confirm the diagnosis and determine the type of LCA in order to give the patient the 

most accurate prognosis possible (approximately 30% of all patients carry a mutation that has not been 

identified to date). 

Approaches for the Treatment of LCA10 

There are currently no disease modifying treatments approved for patients with p.Cys998X associated LCA10 

and disease management is currently supportive in nature. The eye is highly suitable for oligonucleotide 

therapies as it is a contained organ with physical cellular barriers. These natural barriers strongly limits the 

free entry and exit of cells and larger molecules in and out of the eye, therefore limiting the systemic 

exposure of locally administered therapies. 

Sepofarsen for LCA10, splice correction for  
p.Cys998X CEP290 mRNA 

Sepofarsen binds to pre-mRNA and silences the cryptic splice 
site leading to production of normal mRNA 

Sepofarsen for the treatment of LCA10 

Sepofarsen (formerly named QR-110) is 

designed to treat LCA10 by splice correction. 

By binding to the pre-mRNA sepofarsen aims 

to silence the cryptic splice site caused by the 

p.Cys998X mutation. The splicing machinery 

can thus process the pre-mRNA correctly 

resulting in normal mRNA and we expect the 

production of full-length functional wild type 

CEP290 protein. Sepofarsen is designed to be 

administered by intravitreal injection. 

Sepofarsen has received orphan drug 

designation from the U.S. FDA and European 

Commission. Sepofarsen was also granted fast 

track designation by the U.S. FDA. 

Clinical Development for Sepofarsen 

The activity seen in our preclinical models of 

LCA10 provided strong support for the clinical 

development and therapeutic potential of 

sepofarsen. The clinical development of 

sepofarsen began in the second half of 2017 

with a Phase 1/2 open-label, multiple dose, 

dose escalation study to evaluate the safety 

and tolerability of sepofarsen, study 

PQ-110-001.  This trial is currently ongoing 

(enrollment complete) and includes five children (age 8 - 17 years) and six adults (≥ 18 years) who have LCA10 

due to one or two copies of the p.Cys998X mutation in the CEP290 gene. Participants were to receive up to 

four intravitreal injections of sepofarsen into one eye; every three months. Based on updated data suggesting 

a longer half-life of sepofarsen in the retina, dosing of patients has been adjusted to once every six months 

 
 
 
 
   
after receiving their first 2 injections 3 months apart. The study is being conducted in three centers with 

significant expertise in genetic retinal disease in the U.S. and Europe. 

The primary objectives of the trial are safety and tolerability. Secondary objectives include the 

pharmacokinetics and restoration/improvement of visual function and retinal structure through ophthalmic 

endpoints such as best-corrected visual acuity (BCVA), full-field stimulus testing (FST), optical coherence 

tomography (OCT), pupillary light reflex (PLR), mobility course and oculomotor instability (OCI). Reports of 

substantial improvement in vision in one subject led to the decision to perform an interim analysis of data 

collected as of August 16, 2018. 

Safety data: 

At the time of the interim analysis (August 16, 2018), treatment-emergent adverse events (TEAEs) reported 

were mostly mild and there had been no signs of intraocular inflammation. Mild local reactions related to the 

injection procedure such as conjunctival hemorrhage were reported; such events are typical with intravitreal 

injection. To support regulatory discussions (in December 2018) related to advancing the program into a 

potential registrational trial, a further safety follow-up was conducted after the interim analysis, in which, 

adverse events observed after longer duration of treatment included mild cystoid macular edema and lens 

opacities. The cystoid macular edema was observed in two patients in the highest dose tested and was 

responsive to standard of care treatment. There were six participants with lens opacities, of which three went 

on to have corrective lens replacement. These events were considered likely related to study medication and 

are consistent with those seen for other ophthalmic and intravitreal oligonucleotide therapies.  Dosing 

adjustments (dose and dosing interval) were made. There have been no discontinuations from the study.   

Efficacy data: 

The interim analysis of efficacy data from PQ-110-001 confirmed clinical proof-of-concept as shown by 

improvement in BCVA and supported by improvement in performance on the mobility course and reduced 

involuntary eye movement (nystagmus). Mechanistic proof-of-concept was confirmed by improvement in FST. 

Importantly, the four endpoints analyzed showed concordant improvement (Table 1). In approximately 60% 

of subjects, multiple independent measures of visual function were improved in the treated eye, but not in 

the contralateral eye.  

Table 1 Summary of Efficacy Endpoints Assessed for the Interim Analysis (Data Cutoff 16 August 2018)  

Endpoint 

Units 

Direction 
Showing 
Improvement 

Responder 
Threshold 

Change from Baseline at Month 3 
Mean (SEM) 

Treated 

Untreated 

Overall 

Best corrected visual acuity 
(ETDRS/BRVT) (n=8) 

Full field stimulus red (FST 
red) (n=7) 

Full field stimulus blue (FST 
blue) (n=7) 

Mobility course (n=7) 

OCI (nystagmus tracking) 
(n=7)  

LogMAR 

↓= improved 

> -0.3 

-0.67 (0.32) 

0.02 (0.05) 

cd/m2 

↓= improved 

-0.74 (0.35) 

-0.23 (0.18) 

cd/m2 

Level 

↓= improved 

↑= improved 

> 2 

-0.91 (0.38) 

2.57 (1.19) 

-0.02 (0.11) 

1.36 (1.04) 

Log10mm 

↓= improved 

-0.14 (0.08) 

-0.04 (0.06) 

Abbreviations: BRVT=Berkeley Rudimentary Vision Test; cd/m2=logarithm of candelas/square meter; ETDRS=Early Treatment 
Diabetic Retinopathy Study; LogMAR=Logarithm of the Minimum Angle of Resolution; OCI = Oculomotor Instability 

 
 
 
 
 
 
 
 
 
 
 
 
Measurements of best corrected visual acuity (BCVA), functional vision (mobility), and nystagmus confirm 

vision improvement in these subjects. In addition, clear improvement in FST was seen at both red and blue 

wavelengths in the treated eye only. 

BCVA is an accepted registration endpoint for treatments of retinal diseases, with a generally-accepted 

threshold for clinically meaningful improvement of -0.3 LogMAR (15 letters on an eye chart). At Month 3, this 

threshold was exceeded in treated, but not untreated eyes, in the overall population, both in adult and 

pediatric subjects. 

Performance on a mobility course was also improved, and nystagmus was reduced. Concordant 

improvement in the mechanistic and functional outcome measures confirm that these observations are due 

to on-target benefits of sepofarsen. Results from the individual endpoints are discussed in more detail below. 

Best Corrected Visual Acuity (BCVA) 

To assess BCVA, either the ETDRS eye charts or BRVT eye charts (for subjects with more severe visual 

impairment) were used. ETDRS is useful up to LogMAR 1.6, and BVRT extends the range to LogMAR 4.0, or 

mere light perception.  

Data from the three-month assessment of BCVA are shown for the available eight subjects in Figure 1. The 

dark and light green bars on the left represent mean (SEM) and median change from baseline, respectively, 

for the treated eye, and the gray bars (undetectable) on the right represent mean (SEM) and median change 

from baseline for the contralateral eye. Red triangles for the median bars represent individual subject values. 

The dotted horizontal line represents the clinically meaningful level of -0.3 LogMAR. 

In the treated eye, both mean and median change from baseline were above the clinically meaningful 

threshold, while the contralateral eye showed no meaningful improvement. As can be seen in Figure 1, 

clinically meaningful improvement was seen in the treated eyes of 5 of the 8 subjects at Month 3, but no 

subject showed clinically meaningful improvement in the contralateral eye. Importantly, some subjects who 

were only able to perceive hand movement were able to read larger letters on the ETDRS eye chart at three-

month. 

Although the study was not powered to show statistical significance, comparison of the mean change from 

baseline in treated eyes to contralateral eyes at three-month was significant (p=0.011; Wilcoxon’s rank-sum 

test). 

 
 
 
Figure 1 Mean (SEM) and Median Change from Baseline in BCVA at Month 3 (Interim Analysis) 

Figure 2 Mean Change from Baseline in BCVA through Month 6 (Interim Analysis) 

Figure 2 shows mean changes over time for all available BCVA measurements for the treated eye (green line) and 
contralateral eye (gray line). The mean for the treated eye increased to a clinically meaningful extent after the loading 
dose, and remained stable thereafter. Clinically meaningful improvements were observed for the treated eye but not 
for the contralateral eye. This figure shows the three-month data for all eight subjects but also includes the six-month 
data for the four patients who had reached six-months at the time of the assessment. 

Full-Field Stimulus Test (FST): 

The FST is a sensitive mechanistic outcome measure. This test is similar to a hearing test, but instead of 

subjects pushing a button when they first hear a progressively louder tone, in FST they push a button when 

they detect a progressively brighter red or blue light flashed across the entire retina. As FST is a very sensitive 

test, it was hypothesized that improvement in FST would be the earliest and most sensitive indication that 

sepofarsen was engaging its target. 

Figure 3 shows the three-month mean (SEM) change from baseline in ability to see both blue and red 

wavelengths. The dark bars represent the treated eye and the lighter bars represent the contralateral eye. 

Improvement was observed in the treated, but not the contralateral eye for both wavelengths. Figure 4 shows 

the stability of the response over time using all available data. Improvement in the treated eye was observed 

to be well maintained. This figure shows the three-month data for seven subjects but also includes the six-

month data for the four patients who had reached six months at the time of the assessment. 

 
 
 
 
 
 
 
Figure 3 Mean (SEM) Change from Baseline  
in Full-field Stimulus Test at Month 3  
(Interim Analysis) 

Figure 4 Mean Change from Baseline  
in Full-field Stimulus Test through Month 6  
(Interim Analysis) 

Mobility Course 

A mobility course suitable for patients with LCA10 was developed to quantify improvements in functional 

vision. The tool involves different layouts of increasing complexity, using multiple light levels. In total, the 

series of courses produces 19 levels, with level 1 being the ability to navigate a short, straight course with a 

single brightly-backlit obstacle; the other end of the spectrum at level 19 is the ability to navigate a very dimly-

lit complex course with multiple obstacles. Improvement is measured by the number of levels a patient is 

able to navigate. 

Figure 5 shows the three-month mean (SEM) change from baseline in number of levels subjects are able to 

navigate. The green bar represents the treated eye and the gray bar represents the contralateral eye. Red 

triangles represent individual subject data points. Figure 6 shows the stability of the response over time using 

all available data. The green line represents the treated eye and the gray line represents the contralateral 

eye. The dotted horizontal line represents the anticipated clinically meaningful threshold for improvement of 

two levels, or approximately a ten-fold reduction in light required for the subject to successfully navigate the 

mobility course.   

Clinically meaningful improvement was seen in the treated eye at three-months. Clinically meaningful 

improvement was also seen in the contralateral eye in some patients at three months. However, the group 

mean for the contralateral eye did not reach the level of being clinically meaningful. Also, this improvement in 

the contralateral eye appears to be transient, as shown in Figure 6. 

Results from the mobility assessment support the functional significance of the best-corrected visual acuity 

improvement. 

 
 
 
 
 
 
 
Figure 5 Mean (SEM) Change from Baseline in 
Mobility Course Results at Month 3  
(Interim Analysis) 

Figure 6 Mean Change from Baseline in Mobility 
Course Results through Month 6  
(Interim Analysis) 

Oculomotor instability (OCI) 

Oculomotor Instability (OCI) (measurement of nystagmus) was also assessed for the interim analysis. 

Nystagmus is involuntary eye movements due to the inability to fixate. Oculomotor Instability quantifies 

nystagmus using laser tracking measurement of eye movement.    

Figure 7 shows the three-month mean (SEM) change from baseline in level of nystagmus. The green bar 

represents the treated eye and the gray bar represents the contralateral eye. Red triangles represent 

individual subject data points. Figure 8 shows the stability of the response over time using all available data. 

The green line represents the treated eye and the gray line represents the contralateral eye. This figure 

shows the three-month data for seven subjects but also includes the six-month data for the four patients 

who had reached six months at the time of the assessment. 

Nystagmus was observed to be improved in the treated eye at three months, compared to both baseline and 

the contralateral eye. This improvement was also noted by study investigators during their initial clinical 

assessment prior to OCI testing. As can be seen in the right panel, improvement in OCI was maintained in the 

treated eye over time, and potentially increased. 

 
 
 
 
 
 
  
Figure 7 Mean (SEM) Change from Baseline in 
Oculomotor Instability at Month 3  
(Interim Analysis) 

Figure 8 Mean Change from Baseline in 
Oculomotor Instability through Month 6  
(Interim Analysis) 

Conclusions from Study PQ-110-001 (Interim Analysis) 

Available data from the interim analysis of PQ-110-001 support the clinical proof-of-concept of sepofarsen as 

shown by improvement in BCVA and supported by improvement in performance on the mobility course and 

reduced involuntary eye movement (nystagmus). Mechanistic proof-of-concept was supported by 

improvement in FST. Importantly, the four endpoints analyzed showed concordant improvement. In 

approximately 60% of subjects, multiple independent measures of visual function were improved in the 

treated eye, but not in the contralateral eye. Treatment-emergent adverse events reported beyond the 

interim analysis were mostly mild except for three lens opacity events that were reported as moderate or 

severe. We intend to conduct further testing of the long-term safety and efficacy of sepofarsen, as well as 

initiation of trials to explore dose response in a controlled manner.  

Next steps in clinical development of sepofarsen 

Study PQ-110-002 is an extension study to continue to provide treatment to subjects completing study 

PQ-110-001 for which the benefit/risk is positive. Study PQ-110-002 will allow for additional assessment of 

long-term safety, tolerability and (systemic) exposure of sepofarsen, as well as efficacy assessments, including 

sustained efficacy. Treatment of the contralateral eye may also be initiated. 

In addition, the ILLUMINATE study (PQ-110-003) will also be initiated. This study is a double-masked, 

randomized, controlled, multiple-dose study to evaluate the efficacy, safety, tolerability and systemic 

exposure of sepofarsen administered via intravitreal injection in subjects with LCA due to the CEP290 

p.Cys998X mutation. ILLUMINATE will include two active dose levels and a sham control group. Efficacy 

assessments, including BCVA, mobility course score, retinal imaging, functional assessments of vision, 

patient-reported outcome (PRO) measures, as well as safety assessments will be performed at selected study 

visits. The primary endpoint will be assessed at 12 months of treatment, but all efficacy and safety 

assessments will continue to be followed during the 24-month treatment period. Treatment of the 

contralateral eye may also be initiated. 

 
 
 
 
 
 
 
 
Beyond sepofarsen we have an additional discovery-stage program, QRX-136, for another mutation in CEP290. 

Preclinical evidence for sepofarsen 

We have conducted in vitro and in vivo preclinical studies that support the clinical development of sepofarsen.  

Sepofarsen assessment in patient fibroblasts 

Since sepofarsen targets the splicing process, the most direct measurable outcome of activity is the profiling 

and quantification of CEP290 transcripts (wild-type and mutant) and protein before and after treatment. In 

preclinical studies, sepofarsen demonstrated restoration of CEP290 wild-type (correctly spliced) mRNA and 

protein in cultured fibroblast cells of LCA10 patients homozygous and compound heterozygous for the 

p.Cys998X mutation.  

Sepofarsen activity in optic cup model 

Optic cups are a retinal organoid model derived from fibroblasts of a LCA10 patient harvested through skin 

biopsies. The cells are reprogrammed into induced pluripotent stem cells, or iPSC, and later differentiated 

into retinal pigmented epithelium cells and neural retinal cells, also known as three-dimensional optic cups. 

The clinical and molecular relevance of the optic cup model, coupled with the absence of an animal model, 

makes the optic cup the best model in which to simulate the mechanisms of LCA10 and effectively test the 

potential of sepofarsen.  

LCA10 patient derived optic cups were exposed to sepofarsen. First, we observed from the results that 

sepofarsen is able to enter the cells without use of any transfection agents. Second, sepofarsen elicited a 

dose-dependent restoration of CEP290 wild type mRNA expression. And third, increased CEP290 mRNA 

expression was also associated with an increase in functional measures such as percentage of ciliated cells 

and the length of the cilia. 

Retinal Distribution of sepofarsen 

Using labelled sepofarsen administered via intravitreal injection into wild type mice eyes, we demonstrated 

that sepofarsen enters the target cells of the retina, including the photoreceptor cells. Sepofarsen has a long 

tissue half-life, with a current estimation of approximately 200 days based on data obtained in a non-human 

primate model for a closely related oligonucleotide. 

 
 
 
 
 
QR-421a and QR-411 for Usher Syndrome Type 2 and non-syndromic retinitis pigmentosa (NSRP)  

Usher Syndrome Type 2 Background 

Usher syndrome is the leading cause of combined deafness and blindness. Patients with this syndrome 

generally progress to a stage in which they have very limited central and peripheral vision and moderate to 

severe deafness. Patients are usually born with moderate to severe hearing loss that may worsen over time. 

The retinal phenotype, known as retinitis pigmentosa, or RP, is characterized by photoreceptor degeneration 

that leads to progressive vision loss. The first visual symptoms typically appear during the second decade of 

life and start with night blindness due to the start of degeneration of rod photoreceptors. When rod 

degeneration progresses, patients lose their peripheral visual field until patients only have a residual central 

island of vision (tunnel vision). Progression of rod degeneration continues with the degeneration of cones 

which eventually results in complete blindness.  

Representation of exon 13  
mutations causing Usher syndrome type 2 

Representation of the PE40  
mutation causing Usher syndrome type 2 

Usher Syndrome Type 2 Genetics 

Usher syndrome type 2 is 

caused by mutations in the 

USH2A gene, encoding the 

protein usherin. Mutations in the 

USH2A gene can disrupt the 

production of usherin, a protein 

expressed in photoreceptors 

where it is required for their 

maintenance. Usherin is also 

expressed in the ear, where it is 

required for normal 

development of cochlear hair 

cells and hence, normal hearing. 

In the eye, defects in usherin 

cause RP. Mutations in USH2A 

can also cause NSRP, in which 

patients experience visual loss 

but do not suffer from hearing 

loss. Exon 13 mutations 

represent the most common 

mutations in the USH2A gene.  

Disease Prevalence and 

Diagnosis 

The diagnosis of the disease is 

based on clinical symptoms and 

ophthalmologic evaluations. A 

genetic screening can determine 

the specific mutation that is 

causing the disease. Although 

accurate prevalence figures do 

not exist, the number of patients 

with vision loss due to USH2A 

exon 13 mutations is estimated 

to be around 16,000 in the 

Western world. In Europe, the 

PE40 mutation is present in 

 
 
 
 
 
 
approximately 3-7% of the total Usher syndrome type 2 population providing us with an estimate of 1,000 

patients in the Western world. This number could be a considerable underestimate as many of these patients 

are unaware of the second disease causing allele following exome sequencing suggesting a causative 

mutation is intronic.  

Approaches for the treatment of Usher Syndrome Type 2  

While the hearing deficit in patients with Usher syndrome type 2 can be at least partially mitigated using 

hearing aids or cochlear implants, there is no approved treatment for the vision loss associated with USH2A 

mutations and disease management is supportive in nature. Vitamin A and docosahexaenoic acid (DHA) 

supplementations have been proposed as pharmacological treatment options. Both therapies have shown a 

good safety profile but limited clinical benefit. We believe QR-421a and QR-411 are the only product 

candidates in development for the treatment of patients with RP caused by mutations in exon 13 or PE40 

mutations in the USH2A gene. Due to the size of the USH2A protein, this type of RP is not amenable to a gene 

therapy approach. Also, given the disease affects both the peripheral and central retina, current limitations of 

the sub retinal procedure used in gene replacement and gene editing approaches, would make those 

approaches not amenable to targeting peripheral diseases. 

QR-421a and QR-411 for the Treatment of Usher Syndrome Type 2 

QR-421a is being developed as a treatment for RP caused by mutations in exon 13 of the USH2A gene. 

Mutations in exon 13, including the prevalent c.2299delG mutation, can disrupt the production of usherin.  

Usherin is required for photoreceptor maintenance. QR-421a aims to induce excision, or skipping, of exon 13 

from USH2A mRNA leading to an in-frame deletion in the USH2A mRNA. Since exon 13 encodes for a repetitive 

part of the usherin protein, excision of exon 13 is expected to lead to a (partially) functional usherin protein. 

Because of the exon skipping approach, QR-421a is not specific to a single mutation but targets any mutation 

present in exon 13 of the USH2A gene. 

Similar to the approach of sepofarsen, QR-411 is targeted at correcting the splicing of a pseudoexon. In 

patients the specific c.7595-2144A>G (PE40) mutation leads to the aberrant inclusion of this pseudoexon in 

the mature mRNA and consequently absence of a functional usherin protein. Correction of splicing with 

QR-411 can lead to restoration of normal, wild-type usherin protein.  

QR-421a USH2A exon 13  
exon skip 

QR-411 USH2A PE40  
splice correction 

 
 
 
 
 
 
 
 
QR-421a and QR-411 have received orphan drug designation from the FDA and EMA. QR-421a was also 

granted fast track designation by the FDA. 

Clinical Development of QR-421a 

We believe that results of preclinical studies provide support for the clinical development and therapeutic 

potential of QR-421a. The QR-421a clinical development program has been initiated with the first-in-human 

STELLAR study (PQ-421a -001), a Phase 1/2 study designed to evaluate the safety and tolerability of a single 

IVT injection of QR-421a in subjects with RP due to mutations in exon 13 of the USH2A gene. A potential dose 

response relationship and duration of effect following a single dose of QR-421a, based on improvements in 

retinal structure or visual field, will also be investigated to inform selection of dose level(s) and dosing 

intervals for subsequent studies. Improvement of visual function and retinal structure will be measured by 

several endpoints such as visual acuity (BCVA), visual field and optical coherence tomography (OCT). Changes 

in quality of life in the trial subjects will also be evaluated.  

A total of 18 adult subjects are planned to be enrolled in three study cohorts, investigating three dose levels 

of QR-421a. Additional dose levels may be evaluated based on ongoing safety and efficacy data monitoring. 

Per dose cohort, a minimum of four subjects will be treated with QR-421a and a minimum of two subjects will 

receive a control sham-procedure. Once the last subject in a dose cohort reaches week 12, an interim analysis 

will be performed to evaluate available safety and efficacy data. QR-421a will be administered by unilateral 

intravitreal injection. Each subject will receive a single dose of QR-421a or sham procedure in their worse eye 

and will be assessed for safety, tolerability and efficacy at follow-up visits. An extension study, which would 

permit continued dosing of eligible subjects who complete PQ-421a-001, is planned. 

An IND has recently been accepted by the FDA for the start of the first-in-human STELLAR study which will be 

conducted at expert sites in North America and Europe. In March 2019, the first patient was dosed in the 

Phase 1/2 STELLAR clinical trial for QR-421a in patients with Usher syndrome type 2 or non-syndromic 

retinitis pigmentosa (RP). 

Design of Phase 1/2 STELLAR Study of QR-421a 

Beyond QR-421a and QR-411 we have an additional discovery-stage program, QRX-461, for another mutation 

in USH2A. 

Preclinical evidence for QR-421a  

In preclinical data we observed: 

  QR-421a induced an in vitro concentration-dependent USH2A exon 13 skip in human retinal organoids 
 

Translation of ush2a ∆exon 13 mRNA into functional Ush2a protein, as confirmed by visualization of 

protein in the photoreceptors and ERG b-wave restoration in zebrafish model; and 

  QR-421a showed rapid clearance from vitreous with prolonged retention and activity in retina in non 

human primates 

 
 
 
 
 
 
 
Concentration-Dependent Increase of USH2A Exon 13 Skip After One Month of  
Exposure to QR-421a in c.2299delG Homozygous Patient Retinal Organoids 

Exon-13 splicing oligos restore ERG in exon-13 mutant fish 

Pharmacokinetics in non human primates 

 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
  
Clinical Development of QR-411 

QR-411 is currently undergoing IND-enabling studies. We plan to advance the QR-411 program towards a 

Phase 1/2 clinical study in 2020. The clinical trial will consist of a single-dose study to determine safety, 

tolerability and efficacy. 

Preclinical evidence for QR-411  
  QR-411-effected splice correction has been observed in patient fibroblasts and two dimensional 

photoreceptor progenitor cells derived from primary fibroblasts of an USH2A c.7595-2144A>G (PE40) 

compound heterozygous patient. 

  QR-411 demonstrates splice correction by the exclusion of human PE40 in a humanized Ush2A zebrafish 

model. 

QR-411 increases wild type USH2A mRNA 

(1)  Expression of wild type (blue bars) and PE40 (black bars) mRNA in a compound heterozygous patient 

fibroblast cell line carrying one allele containing the PE40 mutation and the other allele an exon 13 mutation 
(c.2391_2392del) after treatment with 10, 25, or 50 nM QR-411.  

QR-411 splice correction of pseudoexon 40 in mutant zebrafish 

 
 
 
 
 
  
 
 
 
 
 
 
QR-1123 for autosomal dominant retinitis pigmentosa (adRP)  

adRP Background 

Retinitis pigmentosa (RP) is a group of hereditary retinal diseases in which patients first experience loss of 

night vision in childhood followed by loss of peripheral vision in young adulthood, and central vision in later 

life which ultimately progresses to complete blindness. The worldwide prevalence of RP is about 1 in 4000 for 

a total of more than 1 million affected individuals. The disease can be inherited as an autosomal-dominant 

(about 30–40% of cases), autosomal-recessive (50–60%), or X-linked (5–15%) trait.  

Autosomal-dominant RP (adRP) is characterized by abnormal, diminished or absent a- and b-waves in the 

electroretinogram (ERG), reduced peripheral vision (visual field) and the presence of visual defects such as 

reduced visual acuity and poor photo- and contrast sensitivity. Symptoms typically start in the early teenage 

years, which include night blindness and reduction of the peripheral vision due to the degeneration of the 

rod photoreceptors. As the disease progresses, cone photoreceptors are also affected, which translates into 
loss of central vision and eventually complete blindness in adulthood.  

adRP Genetics 

Mutations in more than 25 genes can cause adRP, but most commonly mutations are found in the rhodopsin 

(RHO) gene, accounting for approximately 25% of adRP cases. The rhodopsin protein is a light sensitive 

pigment that is present in the rod photoreceptors in the retina. Rhodopsin, when exposed to light, undergoes 

conformational changes that are converted into an electrical signal which is sent to the brain where it is 

interpreted as vision. In the United States, the most prevalent mutation associated with adRP is the P23H 

mutation (also known as c.68C>A) in the RHO gene. The mutant P23H rhodopsin protein is misfolded and 

toxic to the rod photoreceptor cells causing loss of vision. Although some wild-type protein is being made, 

there is substantial evidence that the mutant P23H rhodopsin protein elicits a dominant-negative mechanism, 

such that it diminishes the function of the wild-type protein. 

Representation of the p.Cys998X mutation causing LCA10 

Disease Prevalence and 

Diagnosis 

In the United States the 

P23H mutation in the RHO 

gene is the most common 

mutation causing adRP 

and affects approximately 

2,500 patients. The 

diagnosis of adRP is 

based on clinical 

symptoms and ophthal-

mologic evaluations. A 

genetic screening can 

determine what specific 

mutation is causing the 

disease. 

 
 
 
 
 
 
 
 
 
Approaches for the treatment of adRP 

We believe QR-1123 is the only candidate in development for the treatment of patients with adRP caused by 

the P23H mutation and disease management is currently supportive. 

QR-1123 for the treatment of adRP 

QR-1123, discovered by Ionis Pharmaceuticals and in-licensed by ProQR in 2018, is designed for the 

treatment of P23H adRP. QR-1123 is a gapmer that aims to suppress the formation of the mutant protein by 

selectively targeting the mutant RNA and causing its destruction by RNase H1 cleavage without affecting the 

wild-type RNA. With reducing the mutant RNA we believe the toxicity-induced loss of the photoreceptors and 

subsequent loss of vision can be stopped or potentially reversed. 

QR-1123 for adRP, mutant specific knock-down  
of P23H mRNA 

Clinical Development of QR-1123 

Currently, the QR-1123 program is 

undergoing the final preparation stages for 

IND submission. We plan to advance the 

QR-1123 program towards a Phase 1/2 

clinical trial during 2019. 

Preclinical evidence for QR-1123 

QR-1123 is specific for P23H mutant RNA 

In vitro and in vivo experiments have been 

performed to study the specificity of 

QR-1123 for the P23H mutant RNA. Cell 

models expressing wild-type or P23H 

mutant human RHO were used to 

determine the selectivity of QR-1123 

induced knock-down of P23H mRNA. QR-

1123 was observed to selectively target the 

human P23H mutant rhodopsin mRNA, 

whilst sparing the human wild-type mRNA 

(Figure 2, left panel). 

Mice expressing either human wild-type or 

P23H RHO were used to determine the 

ability of QR-1123 to selectively target the 

P23H mutant mRNA in vivo following 

intravitreal delivery. The mice were treated 

with either QR-1123 or a control and the 

other (contralateral) eye was injected with 

saline solution and used as a comparator control. As expected, in mice expressing wild-type RHO, no 

difference was observed between the two study groups (Figure 2, right panel) while mutant P23HRHO mRNA 

was reduced after a single QR-1123 injection in the hP23HTg mice eyes (Figure 2, center panel) confirming the 

specificity for the P23H allele. 

 
 
 
 
Figure 2: Transgenic mice expressing human P23H RHO treated with QR-1123 or a control; data are presented as 
percent of contralateral eye PBS-treated RNA level. Right Panel: Transgenic mice expressing human wild-type (WT) RHO 
treated with QR-1123 or a control; data are presented as percent of contralateral eye PBS-treated RNA level. 

QR-1123 surrogate improves ERG in P23H rat model  

A rat model of P23H adRP undergoes degeneration and photoreceptor cell loss that is generally characteristic 

of human P23H adRP although the degeneration in these rats is more aggressive than is observed in humans. 

Approximately 25% of photoreceptor cells are lost by Day 15 in these animals, and there are few functional 

photoreceptor cells by 29 weeks of age. Rats received saline in their left eyes and either QR-1123 surrogate or 

control intravitreal treatment in the right eyes once on Day 10 and again on Day 21 after birth. On Day 42 (32 

days following the first injection) the rats’ photoreceptor cell response was measured by ERG. The rats given 

QR-1123 surrogate had an improved scotopic a-wave response amplitude at all stimulus intensities (Fig. 3, left 

panel). This improved response was not observed in the control-treated eyes (Fig. 3, left panel).   

Figure 3: ONL preservation and ERG improvement after QR-1123 treatment. Left Panel: Representative retinal 
micrographs of P23H-1 rhodopsin transgenic rat eyes from the PBS or QR-1123 surrogate-treated eye 30 days post IVT 
injection. Right Panel: Improved ERG response in P23H-1 transgenic rats after a single QR-1123 surrogate treatment with 
IVT injections at P13 (A) or P14 (B), with ERG measurements made at P48. (A, B).  Amplitude versus stimulus intensity 
curves for scotopic a-waves (circles).  The scotopic a-waves of eyes injected with QR-1123 surrogate were significantly 
greater than PBS-injected contralateral eyes while eyes treated with control were similar to those of PBS-injected 
contralateral eyes (t-test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). In the data points without apparent error 
bars, the error bars are obscured by the symbol.   

QR-1123 reduces retinal degeneration in mouse model 

A mouse model of P23H adRP shows degeneration of photoreceptor cells in the retina (reduced cell rows in 

the outer nuclear layer (ONL)) at about 3 months of age. A single intravitreal (IVT) administration of QR-1123 

retarded the progressive retinal degeneration, as measured at 60 days after the single treatment (Figure 4, 

 
 
 
 
 
 
 
 
 
 
 
top panel). Importantly, the activity was observed throughout all regions of the retina (Figure 4, lower 

panel). This shows that QR-1123 has the capability to stop retinal degeneration and indicates that a 

mechanism based on inhibition of the formation of toxic mutant version of rhodopsin protein has the 

potential to improve a clinically relevant functional outcome in RP.   

Figure 4: Preservation of ONL in a Tg mouse model after treatment with QR-1123. Top panel: Depicted is a spider diagram 
of the outer nuclear layer measurements of the entire retina of eyes treated with either PBS (red line) or QR-1123 treated 
eyes (Green line). Lower Left panel: Average superior region ONL thickness at baseline and in PBS and QR-1123 treated 
mice. Lower Right panel: Average inferior region ONL thickness at baseline and in PBS and QR-1123 treated mice. Two-
tailed t test; **p < 0.01, ****p<0.0001.   

Human resources 

At ProQR we have set ourselves the immense task of developing drugs that will potentially transform the lives 

of patients suffering from severe genetic diseases like cystic fibrosis, Leber’s congenital amaurosis, and 

epidermolysis bullosa. To make this happen we demand the utmost of ourselves. We actively create a caring 

atmosphere filled with fun and joy, in which we love to work and maintain productive and happy lives. At 

ProQR we foster empowerment, self development, creativity and a sense of community. 

We are a supportive, ingenious and persistent team that does things different. We're passionate and driven 

to change the lives of patients and their loved ones. 

Corporate social responsibility 

It is required by regulatory authorities to demonstrate the safety and efficacy of a new drug in animals, 

before its efficacy and safety can be tested in humans. ProQR attaches great importance to the welfare of 

 
 
 
  
 
 
animals in our preclinical studies for reasons of ethics, quality, reliability and applicability of scientific studies. 

For conducting high quality (scientific) animal research, animal welfare is a prerequisite. By actively pursuing 

the 3R principles (Reduce, Refine and Replace), ProQR is committed to reduce the number of animals needed, 

minimize discomfort and pain of animals used, and use alternatives to animal research whenever possible.  

Animal experiments will be performed only if there are no alternatives such as performing in silico, in-vitro or 

ex-vivo studies. On a case by case basis, study designs of animal studies will be evaluated with the aim to 

identify opportunities for reduction of the number of animals needed to achieve the objectives of the study. 

By the conduction of small pilot (tolerability) studies first, or by using new technologies to achieve adequate 

statistical power without increasing the number of animals, by combining studies and by improving the use of 

toxicokinetic and modelling data to optimize dose selection, ProQR further pursues the ambitions to reduce, 

refine and replace animal studies. Approval by the (institutional or national) animal care and use committees 

is required prior the execution of in vivo studies.  

External collaborators contracted for the execution of our in-vivo preclinical studies (contract research 

organizations, CROs) are selected based on their expertise, quality and accreditations for laboratory animal 

care and welfare. CRO facilities are audited in person prior contracting to ensure that the housing, husbandry 

and animal welfare complies with the highest international standards. Personnel responsible for housing, 

husbandry and care of the animals must have received adequate and relevant documented education.  

In 2015 ProQR became part of an interdisciplinary consortium with Utrecht University (Faculty of Veterinary 

medicine and Ethics Institute), Radboud University (Medical Center, SYRCLE) and another private company, 

partly financed by The Netherlands Organization for Scientific Research, Responsible Innovation grant. The 

project proposes a more integrated approach towards innovation in the field of animal testing and focuses 

on translational strategies. ProQR is involved in the work package that aims to deliver step stones for 

practical guidelines to build robust translational strategies, to design innovative experiments (including 

animal models) for cystic fibrosis and other rare genetic diseases. 

Manufacturing and Supply  

We do not currently own or operate manufacturing facilities for the production of clinical or commercial 

quantities of any of our product candidates. We currently contract with drug product manufacturers for the 

production of sepofarsen solution for intravitreal injection, QR-421a solution for intravitreal injection and QR-

1123 for intravitreal injection, and we expect to continue to do so to meet the planned clinical requirements 

of our product candidates.  

Currently, each of our active ingredients for our manufacturing activities are supplied by single source 

suppliers. We have agreements for the supply of such active ingredients with manufacturers that we believe 

have sufficient capacity to meet our demands. In addition, we believe that adequate alternative sources for 

such supplies exist. We typically order clinical supplies and services on a purchase order basis and do not 

enter into long-term dedicated capacity or minimum supply arrangements.  

We do have clinical manufacturing processes developed at a back-up GMP manufacturer. Manufacturing is 

subject to extensive regulations that impose various procedural and documentation requirements, which 

govern record keeping, manufacturing processes and controls, personnel, quality control and quality 

assurance, amongst others. The contract manufacturing organizations we use manufacture our product 

candidates under cGMP conditions. cGMP is a regulatory standard for the production of pharmaceuticals that 

will be used in humans. 

 
 
 
 
Competition  

The pharmaceutical industry is highly competitive and subject to rapid and significant technological change. 

Our potential competitors include large pharmaceutical, biotechnology, specialty pharmaceutical, and generic 

drug companies, academic institutions, government agencies and research institutions. Key competitive 

factors affecting the commercial success of our product candidates are likely to be efficacy, safety and 

tolerability profile, delivery, reliability, convenience of dosing, patient recruitment for clinical studies, price 

and reimbursement. Many of our existing or potential competitors have substantially greater financial, 

technical and human resources than we do and significantly greater experience in the discovery and 

development of product candidates, obtaining FDA, EMA and other regulatory approvals of products and the 

commercialization of those products. Mergers and acquisitions in the pharmaceutical and biotechnology 

industries may result in even more resources being concentrated among a small number of our competitors. 

Accordingly, our competitors may be more successful than we may be in obtaining FDA or EMA approval for 

therapies and achieving widespread market acceptance. Our competitors’ products may be more effective, or 

more effectively marketed and sold, than any product candidate we may commercialize and may render our 

therapies obsolete or non-competitive before we can recover development and commercialization expenses.  

Our competitors are working on similar technologies in the field of RNA repair and RNA editing, but also in 

the field of gene editing and gene therapy as well as other types of therapies, such as small molecules, 

protein replacement or antibodies. The industry targeting hereditary ophthalmology indications is driven by 

gene therapy (Spark Therapeutics/Genable, AGTC, Sanofi, Oxford Biomedica), gene editing (Editas Medicine; 

ciberer, OxfordBioMedica and Harvard Medical School), and other approaches (Wave life sciences). 

In the field of DEB, a number of companies are seeking to identify and develop drugs. There are four general 

clusters of potential disease modifying treatments for RDEB: autologous gene therapies (Krystal Biotech, 

Abeona, Fibrocell, King’s Collegeand Holostem Terapie Avanzate), allogeneic cell therapies (Allogeneic Cell 

Therapies, University of Minnesota, Anterogen and King’s College), RNA modulation therapies (University of 

Southern California) and protein replacement therapies (Phoenix Tissue Repair). In regards to palliative 

treatments, the therapies that are currently under development are symptomatic and focus on reducing a 

secondary EB manifestation (Amicus, Amryt, and Tarix Orphan). 

Main financial developments 

Financial position 

In 2018, our operating costs were in line with last year while our liquidity and solvency went up due to an 

increase in cash and cash equivalents. At December 31, 2018, ProQR’s cash and cash equivalents amounted 

to € 105,580,000 compared to € 48,099,000 at December 31, 2017. During the year 2018, operating cash used 

amounted to € 28,493,000, compared to € 34,951,000 in 2017. Total equity increased to € 92,685,000.  

As at December 31, 2018, we had borrowings of € 9,386,000, which consisted of borrowings from a 

government body and convertible loans. Based on the current state of affairs and existing funding, taking into 

account our current cash position and projected cash flows, it is justified that the financial statements are 

prepared on a going concern basis. 

Income statement 

We have generated losses since our formation in February 2012. For the years ended December 31, 2018 and 

2017, we incurred net losses of € 37,086,000 and € 43,675,000, respectively. As at December 31, 2018, we had 

an accumulated deficit of € 155,443,000. We expect to continue incurring losses for the foreseeable future as 

we continue our pre-clinical studies of our product candidates, continue clinical development of our product 

candidates sepofarsen and QR 421a, advance QR-1123 into clinical development, increase investments in our 

other research programs, apply for marketing approval of our product candidates and, if approved, build a 

sales and marketing infrastructure for the commercialization of our product candidates. To date, we have not 

 
 
 
generated any revenues from royalties or product sales. Based on our current plans, we do not expect to 

generate royalty or product revenues for the foreseeable future. 

Other income is incidental by nature. In 2015, the European Commission (EC) through its Horizon 2020 

program awarded us and our academic partners a grant of € 6 million to support the clinical development of 

eluforsen, formerly known as QR-010 (ProQR: € 4.6 million). On February 9, 2018, the Company entered into a 

partnership agreement with Foundation Fighting Blindness (FFB), under which FFB has agreed to provide 

funding of $ 7.5 million for the pre-clinical and clinical development of QR-421a for Usher syndrome type 2A 

targeting mutations in exon 13. On June 5, 2018, the Company entered into a partnership agreement with EB 

Research Partnership (EBRP) and EB Medical Research Foundation (EBMRF) under which EBRP and EBMRF 

have agreed to provide funding of $ 5.0 million for the clinical development of QR-313 for Dystrophic 

Epidermolysis Bullosa targeting mutations in exon 73. Other income amounted to € 5,761,000 compared to 

€ 1,495,000 in 2017. We expect to continue generating other income from new grant applications in 2019. 

Research and development costs amount to € 29,514,000 in 2018 compared to€ 31,153,000 in 2017. These 

research and development costs comprise allocated employee costs including share-based payments, the 

costs of materials and laboratory consumables, the costs for production of clinical and pre-clinical 

compounds and outsourced activities, license and intellectual property costs and other allocated costs. These 
costs were primarily related to our product candidates, sepofarsen, QR‑313 and QR‑421a, and our innovation 

unit. Our research and development expense is highly dependent on the development phases of our product 

candidates and is expected to stay at the same level, although it may fluctuate significantly from period to 

period. 

Costs were incurred for the advancement of our pipeline, which included clinical development of sepofarsen 
eluforsen and QR‑313, preclinical development of QR‑421a and progress of our innovation programs. The 

variances in research and development costs between the years ended December 31, 2018 and 2017 are 

mainly due to:  

 

 

 

 

 

 

 

 

 

costs we incurred on clinical trials for sepofarsen, particularly in 2018; 

costs we incurred on clinical trials for eluforsen, particularly in 2017, decreasing in 2018 after completion 

of the clinical studies. No additional clinical study activities are planned; 

slightly decreased staff costs. The number of full-time equivalent employees working on research and 

development decreased from 96 at December 31, 2017 to 89 at December 31, 2018;  

In November 2018, the Company issued 112,473 shares in the aggregate amount of $ 2.5 million, at 

$ 22.23 per share to Ionis. Under the terms of the agreement made an upfront payment in ordinary 

shares to its common stock, to Ionis upon signing the worldwide license agreement. The Company was 

granted an exclusive worldwide license to QR-1123 and relevant patents. The Company will also make 

future milestone payments, certain of which will be made in equity and others in cash or equity at the 

company’s discretion, and royalties on net sales of 20% through the royalty term. 

costs for externally conducted studies, including various in vivo studies, proof of concept studies and 

dose ranging and toxicity studies conducted in connection with the development of our product 

candidates;  
costs for the production of QR‑313 and QR‑421a compounds in 2017, including the costs of GMP batches 

in preparation of our clinical studies;  

laboratory costs including purchases of compounds and laboratory materials used by the research and 

development staff in proportion to the decrease in the number of employees, and increased costs for the 

use of laboratories;  

project-related consultancy costs, including regulatory and intellectual property support; and  

decreased share-based compensation, reflecting grants of share options to research and development 

staff made after we adopted our Option Plan in September 2013.  

 
 
 
 
General and administrative costs increased to € 12,540,000 in 2018 from € 10,840,000 in 2017. These general 

and administrative costs comprise employee costs, office costs, general consultancy costs and other costs. As 

a public company, we face increased legal, accounting, administrative and other costs and expenses. The 

increase was primarily related to:  

 

 

 

increased office and general costs, including office rent, information technology and communication 

costs, travel costs and office consumables, as well as costs to improve our internal control environment;  

increased costs for legal support, accounting and other consultancy costs, including costs incurred in 

preparation of offerings in 2018; and 

decreased share-based compensation, reflecting grants of share options to non-research and 

development staff made after we adopted our Option Plan in September 2013. 

In 2018 share-based compensation amounted to € 3,224,000, compared to € 4,024,000 in 2017. Net financial 

expenses amounted to € 792,000, compared to € 3,175,000 in 2017. Financial income & expenses mainly 

result from foreign exchange differences on cash denominated in U.S. dollars and can fluctuate significantly. 

The Company operates a foreign exchange policy to manage the foreign exchange risk against the functional 

currency based on the Company’s cash balances and the projected future spend per major currency. 

Outlook 

In 2019, we continue to invest in our organization, while we continue our pre-clinical studies and clinical 

development of our product candidates and increase investments in our other research programs. Our goal 

is to realise this at our current operational level. A significant increase in headcount is not expected. We 

believe we have sufficient cash to fund these expenses and to prepare the Company for future growth. Given 

the development stage of the Company, we do not anticipate revenues in the foreseeable future. 

Leiden, March 28, 2019 

On behalf of the Management Board, 

Daniel de Boer 

CEO 

 
 
 
 
ProQR Therapeutics has chosen for its governance structure to be a so-called two-tier system. In such a 

setting the Supervisory Board supervises and advises the Management Board in performing their 

management tasks and setting the strategy of the Company. The Supervisory Board as well as its individual 

members act in the interests of ProQR, its business and development and all its stakeholders. 

The Supervisory Board and its sub-committees held frequent and productive interactions with the Executive 

Board. Where appropriate, decision taking was endorsed by the Supervisory Board and matters of both short 

term as well as long term strategic importance were discussed in a constructive and transparent manner. 

Below is a more specific description of the Supervisory Board’s activities during the financial year 2018 and 

other relevant information on its functioning. 

Activities of the Supervisory Board 

The Supervisory Board and the Board of Directors met multiple times during 2018 and have held various 

additional informal meetings and telephone conferences, both collectively and individually. During these 

meetings, the progress of the various projects, the main risks of the business, the funding and the strategic 

direction of the Company were discussed. In addition, a two-day off-site was held during which the long-term 

strategy of the company was discussed. The Supervisory Board meetings were very well attended and the 

Committees reported back on their activities to the full Supervisory Board on a regular basis. 

Committees of the Supervisory Board 

We have an audit committee, a compensation committee and a nominating and corporate governance 

committee. We have adopted a charter for each of these committees.  

Compensation Committee 

The Compensation Committee met 2 times in 2018. 

Compensation report 2018 

In June 2016, the General Meeting of Shareholders adopted our Compensation Policy. This Compensation 

Policy also applied to the financial year 2018 and will apply to subsequent years. Attraction and retention of 

world class talent is a prerequisite for the success of ProQR and competitive compensation plays a vital role 

in our ability to achieve this. The Compensation Committee elected to offer compensation for all employees 

including the Management Board into a fixed annual salary and a variable, performance related, short and 
long term incentive element. The Compensation Policy is designed based on the following principles: 

 

Three compensation pillars consisting of: 

  Annual Base Salary; 
 

Short Term Incentive (annual cash bonus); 

 

Long Term Incentive (Stock Option Plan); 

 

 

 

Flexibility: The Compensation Policy should provide flexibility to allow the Supervisory Board, acting on 

the recommendation of the Compensation Committee, to reward the Management Board in a fair and 

equitable manner; 

This Compensation Policy should drive the right kind of management behavior, discourage unjustified 

risk taking and minimize any gaming opportunity; 

This Compensation Policy should pay for performance, considering not only the measurable financial 

performance of / or milestones achieved by the Company, but also, where appropriate, the efforts made 

 
 
 
 
by the Management Board, individually and as a group, in managing the Company. For the variable 

components, the Compensation Committee performs an analysis of the possible outcomes under 

different scenarios; 

  Design of the Compensation Policy shall be based on current legislation applicable in the Netherlands; 
 

This Compensation Policy shall foster alignment of interests with shareholders;  

 

 

The pension of the Management Board shall be based on the defined contribution system; and 

Pay differentials and position within the Company are considered and evaluated regularly.  

Annual Base Salary 

The Compensation Committee reviewed the annual base salary of the Management Board taking into 

consideration the Compensation Reference Group as contained in the Compensation Policy. Based on this 

review the annual base salary levels for 2019 have been set at € 404,000 for the CEO, Daniel de Boer. René 

Beukema resigned as per the end of 2018 and received a severance payment of € 324,000. 

Short Term Incentive  

The Compensation Committee reviewed the performance of the Company during 2018 in comparison to the 

objectives and reviewed the achievements of the members of the Management Board versus their personal 

objectives.  

Based on the recommendation of the Compensation Committee, the Supervisory Board decided late 2018 

that the Company has achieved 125% of the objectives that had been set to determine the individual bonus 

awards for the year 2018. For 2018 the individual bonus has been set at € 281,000 for Daniel de Boer and at 

€ 134,000 for René Beukema. Final installment of these bonuses will be paid in cash in the first quarter of 

2019.  

Long Term Incentive 

Based on the recommendation of the Compensation Committee, the Supervisory Board decided to grant 

stock options in 2018 to the CEO, Daniel de Boer and the chief corporate development officer and general 

counsel, René Beukema. Based on this decision stock options with an exercise price of € 2.74 have been 

granted with respect to 379,285 shares to the CEO, Daniel de Boer and 140,932 shares to the chief corporate 

development officer and general counsel, René Beukema.  

Pensions 

The pension contributions paid during 2018 amount to € 9,000 for the CEO, Daniel de Boer and € 16,000 for 

the chief corporate development officer and general counsel, René Beukema. 

Internal pay ratio 

The internal pay ratio between the average pay of our employees and our Management Board is calculated 

based on the average remuneration based on short term and long term incentives. The pay ratio is 8:1 for 

2018. 

Supervisory Board remuneration 

In June 2016, our shareholders approved an amended compensation policy whereby members of our 

Supervisory Board receive board fees of € 25,000 per year and the chairperson receives board fees of 

€ 30,000 per year. In addition, each board committee chairperson receives € 5,000 per year for service on 

such committee (except for the chairperson for the nominating committee who receives € 3,000), and each 

other member of a board committee receives € 3,000 per year for service on such committee. On top of that, 

Supervisory Board members were granted options as set out in Note 23 to the financial statements or 

$ 55,000 in cash. 

 
 
 
Nominating and Corporate Governance Committee 

The Supervisory Board assessed its composition in 2018. It was concluded that the composition of the 

Supervisory Board is satisfactory and appropriate for current the phase of the company. Looking forward the 

supervisory board expressed a desire to seek three new candidate members over the next 18 months with 

knowledge and/or expertise of rare disease, ophthalmology, and/or commercialization and a candidate who 

is capable of taking over the chair position of the audit committee. 

Audit Committee 

The audit committee met 5 times in 2018. Main topics addressed were the quarterly results, financial risk 

management, compliance and SOx implementation, the audit plan and management letter of the external 

auditor, cash management, tax and corporate governance. 

The audit committee also reviewed ProQR’s annual financial statements, including non-financial information, 

prior to publication thereof. These financial statements for 2018 have been audited and provided with an 

unqualified opinion by our external auditor, Deloitte Accountants B.V., and were extensively discussed with 

the auditors in the meetings of the Supervisory Board, Audit Committee and Management Board on March 

26, 2019. The Supervisory Board is of the opinion that the Financial Statements 2018 meet all requirements 

and recommends that the Annual General Meeting of Shareholders adopts the financial statements and the 

appropriation of net result proposed by the Management Board. 

The Company’s external auditor attended all Audit Committee meetings. The Audit Committee evaluates the 

performance of Deloitte as independent external auditor annually. Due to the limited size of the Company, it 

was concluded that there was currently no need to appoint an internal auditor. 

The Supervisory Board is responsible for the quality of its own performance and it discusses, once a year on 

its own, without the members of the Management Board present, both its own functioning and that of the 

individual members, and the functioning of the Management Board and that of its individual members. The 

Supervisory Board discussed its functioning and competencies and concluded that it’s functioning and 

competencies are appropriate for the current phase of the company, but expressed an intent to seek new 

candidates as described above. The performance and composition of the Management Board were also 

found to be adequate. We feel the additional efforts of all staff at ProQR form a strong foundation for the 

success and growth of the Company and all milestones reached this past year. Therefore, we would like to 

express our thanks to the members of the Management Board, senior management and all other employees 

for their contribution and performance during the year. The Supervisory Board is grateful for René 

Beukema’s invaluable contributions and dedication to ProQR as a member of our management Board. His 

commitment has been exemplary for all ProQRians. We thank our shareholders for their continued support. 

Leiden, March 28, 2019 

On behalf of the Supervisory Board, 

Dinko Valerio 

Chairman 

 
 
 
 
 
ProQR values the importance of complying with Corporate Governance regulations. At the same time, the 

Board of Directors is of the opinion that certain deviations from the provisions of the new Dutch Corporate 

Governance Code 2016 (“DCGC” or “the Code”) are justified, in view of our activities, our size and the specific 

circumstances in which we operate. In such cases, which are mentioned in this corporate governance 

statement, we apply the “comply or explain” principle.  

Deviations from certain aspects of the Code, when deemed necessary in the interests of the Company, will be 

disclosed in the Annual Report. Most deviations are justified due to our Company being listed in the United 

States with most of our investors being outside of the Netherlands, as well as to the international business 

focus of our Company. As a Company listed on NASDAQ, we comply with NASDAQ’s corporate governance 

listing standards, except for instances where we follow our home country’s corporate governance practices in 

lieu of certain NASDAQ’s standards as explained below, as NASDAQ investors are more familiar with 

NASDAQ’s rules than with the Code. 

In this report, the Company addresses its overall corporate governance structure and states to what extent 

and how it applies the principles and best practice provisions of the Code. This report also includes the 

information which the Company is required to disclose pursuant to the Dutch governmental decree on Article 

10 Takeover Directive and the governmental decree on Corporate Governance.  

Substantial changes in the Company’s corporate governance structure and in the Company’s compliance with 

the DCGC, if any, will be submitted to the General Meeting of Shareholders for discussion under a separate 

agenda item. The Supervisory Board and the Management Board, which are responsible for the corporate 

governance structure of the Company, are of the opinion that the principles and best practice provisions of 

the DCGC that are addressed to the Management Board and the Supervisory Board, interpreted and 

implemented in line with the best practices followed by the Company, are being applied.  

The full text of the DCGC can be found at the website of the Monitoring Commission Corporate Governance 

Code (www.mccg.nl) and for an overview of our conformity with the Code the following documents are 

available at our website (www.ProQR.com): audit committee charter, compensation committee charter, 

nominating and corporate governance committee charter and our code of business conduct and ethics. 

Management Board 

ProQR is dedicated to improve the lives of our patients and their loved ones through the development of RNA 

therapies for severe genetic orphan diseases. ProQR has a focus on patients with LCA, Usher and EB. The 

expectations and interests of our stakeholders is a key reference point in establishing our long term strategy. 

The Management Board’s role is to develop long term value creation by means of a strategy to pursue the 

long term success of ProQR. The strategy contains multiple elements linked to the new Corporate 

Governance Code:  

 

Implementation and feasibility; 

  Business model applied by the company; 
  Opportunities and risks; 
  Operational and financial objectives; 

 

Interest of shareholders; 

  Any other relevant aspects such as environment, charity and patient organizations. 

 
 
 
The Management Board executes the strategy by assuming the authority and responsibilities assigned to it by 

Dutch corporate law and by combining expertise and experience with entrepreneurial leadership. The 

Management Board operates under the supervision of the Supervisory Board. The Management Board is 

required to:  

 

Keep the Supervisory Board informed in a timely manner in order to allow the Supervisory Board to carry 

out its responsibilities;  

  Consult with the Supervisory Board on important matters; and  
 

Submit important decisions to the Supervisory Board for its approval.  

Our Management Board may perform all acts necessary or useful for achieving our corporate purposes, 

other than those acts that are prohibited by law or by our articles of association. The Management Board as a 

whole and any Management Board member individually, are authorized to represent us in dealings with third 

parties.  

Under our articles of association, the number of Management Board members is determined by the 

Supervisory Board, and the Management Board must consist of at least one member. The Supervisory Board 

elects a CEO from among the members of the Management Board.  

Members of the Management Board are appointed by the general meeting of shareholders upon a binding 

nomination of the Supervisory Board. Our general meeting of shareholders may at all times deprive such a 

nomination of its binding character by a resolution passed by at least two-thirds of the votes cast 

representing more than 50% of our issued share capital, following which our Supervisory Board shall draw up 

a new binding nomination.  

Our Management Board rules provide that, unless the resolution appointing a Management Board member 

provides otherwise, members of our Management Board will serve for a maximum term of four years. Our 

articles of association provide that the Management Board members must retire periodically in accordance 

with a rotation schedule adopted by the Management Board. A Management Board member who retires in 

accordance with the rotation schedule may be reappointed immediately for a term of not more than four 

years at a time.  

In view of the resignation of Mr Beukema a new succession plan for Management Board members will be 

established by the Supervisory Board in 2019 that is aimed at retaining the requisite expertise, experience 

and diversity. 

Supervisory Board  

Our Supervisory Board is responsible for the supervision of the activities of our Management Board and our 

Company’s general affairs and business. Our Supervisory Board may, also on its own initiative, provide the 

Management Board with advice and may request any information from the Management Board that it deems 

appropriate. In performing its duties, the Supervisory Board is required to act in the interests of our Company 

(including its stakeholders) and its associated business as a whole. The members of the Supervisory Board 

are not authorized to represent us in dealings with third parties.  

Pursuant to Dutch law, members of the Supervisory Board must be natural persons. Under our articles of 

association, the number of Supervisory Board members is determined by our Supervisory Board itself, 

provided there will be at least three Supervisory Board members. Our articles of association provide that 

members of the Supervisory Board are appointed by the general meeting of shareholders upon a binding 

nomination by the Supervisory Board. Our general meeting of shareholders may at all times deprive such a 

nomination of its binding character by a resolution passed by at least two-thirds of the votes cast 

 
 
 
 
representing more than 50% of our issued share capital, following which our Supervisory Board shall draw up 

a new binding nomination.  

Our Supervisory Board rules provide that members of our Supervisory Board will serve for a maximum 

duration of three terms of four years. Our articles of association provide that the Supervisory Board 

members must retire periodically in accordance with a rotation schedule adopted by the Supervisory Board. 

A Supervisory Board member who retires in accordance with the rotation schedule can be reappointed 

immediately. The Supervisory Board appoints a chairman from among its members.  

With the exception of Dinko Valerio and Antoine Papiernik, each member of our Supervisory Board has been 

and remains fully independent within the meaning of best practice provision 2.1.8 of the DCGC. Mr. Dinko 

Valerio has provided a convertible loan to Amylon Therapeutics B.V. This loan becomes payable on demand 

after 24 months in equal quarterly terms. Mr. Papiernik is affiliated with Sofinnova which holds 7.1% of our 

shares. Both are therefore not independent within the meaning of best practice provision 2.1.8 of the Code. 

We feel their membership of the supervisory board is justified by their specific knowledge and experience of 

our business. Moreover, we do comply with best practice provision 2.1.7 of the DCGC, as only two out of 5 

supervisory board members are not independent under best practice provision 2.1.8 of the Code and they 

are so under different criteria of said provision 2.1.8. 

Under our articles of association, the general meeting of shareholders may suspend or remove Supervisory 

Board members at any time. A resolution of our general meeting of shareholders to suspend or remove a 

Supervisory Board member may be passed by a simple majority of the votes cast, provided that the 

resolution is based on a proposal by our Supervisory Board. In the absence of a proposal by our Supervisory 

Board, a resolution of our general meeting of shareholders to suspend or remove a Supervisory Board 

member shall require a majority of at least two-thirds of the votes cast representing more than 50% of our 

issued share capital.  

In a meeting of the Supervisory Board, each Supervisory Board member is entitled to cast one vote. A 

Supervisory Board member may grant a written proxy to another Supervisory Board member to represent 

him at a meeting of the Supervisory Board. All resolutions by our Supervisory Board are adopted by a simple 

majority of the votes cast unless our Supervisory Board rules provide otherwise. In case of a tie in any vote of 

the Supervisory Board, the chairman of the Supervisory Board shall have the casting vote. Our Supervisory 

Board may also adopt resolutions outside a meeting, provided that such resolutions are adopted in writing, 

all Supervisory Board members are familiar with the resolution to be passed and provided that no 

Supervisory Board member objects to such decision-making process. 

A succession plan for Supervisory Board members is in place that is aimed at retaining the balance in the 

requisite expertise, experience and diversity. 

Committees of the Supervisory Board  

We have an audit committee, a compensation committee and a nominating and corporate governance 

committee. We have adopted a charter for each of these committees.  

Audit Committee  

Our audit committee consists of Paul Baart (chairman), Alison Lawton and James Shannon. Each member 

satisfies the independence requirements of the NASDAQ listing standards / Rule 10A-3(b)(1) under the 

Exchange Act, and each member meets the criteria for independence set forth in best practice 2.1.8 of the 

DCGC. Paul Baart qualifies as an “audit committee financial expert,” as defined by the SEC in Item 16A: “Audit 

Committee Financial Expert” and as determined by our Supervisory Board. The audit committee oversees our 

 
 
 
accounting and financial reporting processes and the audits of our financial statements. The audit committee 

is responsible for, among other things:  

 

 

 

 

 

 

 

 

the operation of the internal risk management and control systems, including supervision of the 

enforcement of relevant primary and secondary legislation, and supervising the operation of codes of 

conduct; 

the provision of financial information by the company (choice of accounting policies, application and 

assessment of the effects of new rules, information about the handling of estimated items in the financial 

statements, forecasts, work of internal and external auditors, etc.); 

compliance with recommendations and observations of internal and external auditors; 

the policy of the company on tax planning; 

relations with the external auditor, including, in particular, his independence, remuneration and any non-

audit services for the company; 

the financing of the company; and 

the applications of information and communication technology, including risks relating to cyber security; 

annually reviewing the need for an internal audit function: 

the Supervisory Board has decided not to create an internal audit function for the time being, since the 

current scope of the business does not justify such a fulltime role. The Supervisory Board has delegated 

an active role to its Audit Committee in the design, implementation and monitoring of internal risk 

management and control system to manage the significant risks to which the Company is exposed. 

Compensation Committee  

Our compensation committee consists of James Shannon (chairman), Dinko Valerio and Alison Lawton. Each 

member satisfies the independence requirements of the NASDAQ listing standards / Rule 10A-3(b)(1) under 

the Exchange Act, and each member meets the criteria for independence set forth in best practice 2.1.8 of 

the DCGC, with the exception of Dinko Valerio, as set forth above. The compensation committee assists our 

Supervisory Board in reviewing and approving or recommending our compensation structure, including all 

forms of compensation relating to our Supervisory Board members, our Management Board members and 

our officers. Members of our Management Board may not be present at any compensation committee 

meeting while their compensation is deliberated. Subject to and in accordance with the terms of the 

compensation policy approved by our general meeting of shareholders from time to time, as required by 

Dutch law, the compensation committee is responsible for, among other things:  

  making a proposal to the Supervisory Board for the remuneration policy to be pursued; 
  making a proposal for the remuneration of the individual members of the Management Board, for 

adoption by the Supervisory Board; such proposal shall, in any event, deal with: (i) the remuneration 

structure and (ii) the amount of the fixed remuneration, the shares and/or options to be granted and/or 

other variable remuneration components, pension rights, redundancy pay and other forms of 

compensation to be awarded, as well as the performance criteria and their application; and 

 

preparing the remuneration report as referred to in best practice provision 3.4.1. 

Our Supervisory Board may also delegate certain tasks and powers under our Option Plan to the 

compensation committee.  

Nominating and Corporate Governance Committee  

Our nominating and corporate governance committee consists of Dinko Valerio (chairman) and Paul Baart. 

Each member satisfies the independence requirements of the NASDAQ listing standards and Paul Baart 

satisfies the criteria for independence set forth in best practice 2.1.8 of the DCGC. The nominating and 

corporate governance committee assists our Supervisory Board in selecting individuals qualified to become 

our Supervisory Board members and Management Board members and in determining the composition of 

 
 
 
 
the Management Board, Supervisory Board and its committees and our officers. The nominating and 

corporate governance committee is responsible for, among other things:  

 

 

 

drawing up selection criteria and appointment procedures for Supervisory Board members and 

Management Board members; 

periodically assessing the size and composition of the Supervisory Board and the Management Board, 

and making a proposal for a composition profile of the Supervisory Board; 

periodically assessing the functioning of individual Supervisory Board members and Management Board 

members, and reporting on this to the Supervisory Board; 

  making proposals for appointments and reappointments; and 
 

supervising the policy of the Management Board on the selection criteria and appointment procedures 

for senior management. 

Insurance and Indemnification of Management Board and Supervisory Board Members  

Under Dutch law, Management Board members, Supervisory Board members and certain other 

representatives may be held liable for damages in the event of improper or negligent performance of their 

duties. They may be held jointly and severally liable for damages to the Company for infringement of the 

articles of association or of certain provisions of the Dutch Civil Code. They may also be liable towards third 

parties for infringement of certain provisions of the Dutch Civil Code. In certain circumstances they may also 

incur additional specific civil and criminal liabilities.  

Our articles of association provide that we will indemnify our Management Board members, Supervisory 

Board members, former Management Board members and former Supervisory Board members (each an 

“Indemnified Person”) against (i) any financial losses or damages incurred by such Indemnified Person and 

(ii) any expense reasonably paid or incurred by such Indemnified Person in connection with any threatened, 

pending or completed suit, claim, action or legal proceedings, whether civil, criminal, administrative or 

investigative and whether formal or informal, in which he becomes involved, to the extent this relates to his 

position with the Company, in each case to the fullest extent permitted by applicable law. No indemnification 

shall be given to an Indemnified Person (a) if a Dutch court has established, without possibility for appeal, 

that the acts or omissions of such Indemnified Person that led to the financial losses, damages, suit, claim, 

action or legal proceedings result from either an improper performance of his duties as an officer of the 

Company or an unlawful or illegal act and (b) to the extent that his financial losses, damages and expenses 

are covered by an insurance and the insurer has settled these financial losses, damages and expenses (or has 

indicated that it would do so). Our Supervisory Board may stipulate additional terms, conditions and 

restrictions in relation to such indemnification.  

Board composition and diversity 

Our Management Board comprised two persons in 2018, both of whom are male. Our Supervisory Board has 

four male members and one female member. As a Company, we support diversity of culture, gender and age 

in our Company. ProQR maintains a culture that reflects that ProQR is a multicultural company representing 

employees from over twenty countries. The culture is represented by the commitment to conducting our 

business ethically and to observing applicable laws, rules and regulations. In this context the Code of Conduct 

and Whistleblower policy are implemented and strongly anchored in the organization. Effectiveness of the 

Code of Conduct is monitored periodically. 

Our current Management Board and Supervisory Board members were selected based on the required 

profile and talent and abilities of the members without positive or negative bias on gender, culture or age. In 

the future, this will continue to be our basis for selection of new Board members. 

 
 
 
General Meeting of Shareholders 

General meetings of shareholders may be held in Leiden, Oegstgeest, Leidschendam, Katwijk, Noordwijk, 

Wassenaar, Amsterdam, Rotterdam, The Hague, or Schiphol Airport (municipality of Haarlemmermeer), the 

Netherlands. All shareholders and others entitled to attend general meetings of shareholders are authorized 

to attend the general meeting of shareholders, to address the meeting and, in so far as they have such right, 

to vote, either in person or by proxy.  

Annually, at least one general meeting of shareholders shall be held, within six months after the end of our 

financial year. A general meeting of shareholders shall also be held within three months after our 

Management Board has considered it to be likely that the Company’s equity has decreased to an amount 

equal to or lower than half of its paid up and called up capital. If the Management Board and Supervisory 

Board have failed to ensure that such general meetings of shareholders as referred to in the preceding 

sentences are held in a timely fashion, each shareholder and other person entitled to attend shareholders’ 

meetings may be authorized by the Dutch court to convene the general meeting of shareholders. 

Our Management Board and our Supervisory Board may convene additional extraordinary general meetings 

of shareholders whenever they so decide. Pursuant to Dutch law, one or more shareholders and/or others 

entitled to attend general meetings of shareholders, alone or jointly representing at least ten percent of our 

issued share capital may on their application, be authorized by the Dutch court to convene a general meeting 

of shareholders. The Dutch court will disallow the application if it does not appear to it that the applicants 

have previously requested that the Management Board or Supervisory Board convenes a shareholders’ 

meeting and neither the Management Board nor the Supervisory Board has taken the necessary steps so that 

the shareholders’ meeting could be held within six weeks after the request.  

General meetings of shareholders are convened by a notice which includes an agenda stating the items to be 

discussed. For the annual general meeting of shareholders the agenda will include, among other things, the 

adoption of our annual accounts, the appropriation of our profits or losses, discharge of the members of the 

Management Board for their management, discharge of the members of the Supervisory Board for their 

supervision on the management and proposals relating to the composition and filling of any vacancies of the 

Management Board or Supervisory Board. In addition, the agenda for a general meeting of shareholders 

includes such items as have been included therein by our Management Board or our Supervisory Board. 

Pursuant to Dutch law, one or more shareholders and/or others entitled to attend general meetings of 

shareholders, alone or jointly representing at least 3% of the issued share capital have the right to request 

the inclusion of additional items on the agenda of shareholders’ meetings. Such requests must be made in 

writing, substantiated, or by a proposal for a resolution and received by us no later than the sixtieth day 

before the day the relevant general meeting is held. No resolutions will be adopted on items other than those 

which have been included in the agenda.  

We will give notice of each general meeting of shareholders by publication on our website and, to the extent 

required by applicable law, in a Dutch daily newspaper with national distribution, and in any other manner 

that we may be required to follow in order to comply with Dutch law, applicable stock exchange and SEC 

requirements. We will observe the statutory minimum convening notice period for a general meeting of 

shareholders.  

Pursuant to our articles of association, our Management Board may determine a record date 

(“registratiedatum”) of 28 calendar days prior to a general meeting of shareholders to establish which 

shareholders and others with meeting rights are entitled to attend and, if applicable, vote in the general 

meeting of shareholders. The record date, if any, and the manner in which shareholders can register and 

exercise their rights will be set out in the convocation notice of the general meeting. Our articles of 

association provide that a shareholder must notify the Company in writing of his identity and his intention to 

 
 
 
 
attend (or be represented at) the general meeting of shareholders, such notice to be received by us ultimately 

on the seventh day prior to the general meeting. If this requirement is not complied with or if upon direction 

of the Company to that effect no proper identification is provided by any person wishing to enter the general 

meeting of shareholders, the chairman of the general meeting of shareholders may, in his sole discretion, 

refuse entry to the shareholder or his proxy holder.  

Pursuant to our articles of association, our general meeting of shareholders is chaired by the chairman of our 

Supervisory Board. If the chairman of our Supervisory Board is absent and has not charged another person 

to chair the meeting in his place, the Supervisory Board members present at the meeting shall appoint one of 

them to be chairman. If no Supervisory Board members are present at the general meeting of shareholders, 

the general meeting of shareholders will be chaired by our CEO or, if our CEO is absent, another Managing 

Board member present at the meeting and, if none of them is present, the general meeting shall appoint its 

own chairman. The person who should chair the meeting may appoint another person in his stead.  

The chairman of the general meeting may decide at his discretion to admit other persons to the meeting. The 

chairman of the general meeting shall appoint another person present at the shareholders’ meeting to act as 

secretary and to minute the proceedings at the meeting. The chairman of the general meeting may instruct a 

civil law notary to draw up a notarial report of the proceedings at the Company’s expense, in which case no 

minutes need to be taken. The chairman of the general meeting is authorized to eject any person from the 

general meeting of shareholders if the chairman considers that person to disrupt the orderly proceedings. 

The general meeting of shareholders shall be conducted in the English language. 

Voting Rights and Quorum Requirements  

In accordance with Dutch law and our articles of association, each issued ordinary share and preferred share 

confers the right on the holder thereof to cast one vote at the general meeting of shareholders. The voting 

rights attached to any shares held by us or our direct or indirect subsidiaries are suspended as long as they 

are held in treasury. Dutch law does not permit cumulative voting for the election of Management Board 

members or Supervisory Board members.  

Voting rights may be exercised by shareholders or by a duly appointed proxy holder (the written proxy being 

acceptable to the chairman of the general meeting of shareholders) of a shareholder, which proxy holder 

need not be a shareholder. Our articles of association do not limit the number of shares that may be voted by 

a single shareholder.  

Under our articles of association, blank votes, abstentions and invalid votes shall not be counted as votes 

cast. Further, shares in respect of which a blank or invalid vote has been cast and shares in respect of which 

the person with meeting rights who is present or represented at the meeting has abstained from voting are 

counted when determining the part of the issued share capital that is present or represented at a general 

meeting of shareholders. The chairman of the general meeting shall determine the manner of voting and 

whether voting may take place by acclamation.  

In accordance with Dutch law and generally accepted business practices, our articles of association do not 

provide quorum requirements generally applicable to general meetings of shareholders. To this extent, our 

practice varies from the requirement of NASDAQ Listing Rule 5620(c), which requires an issuer to provide in 

its bylaws for a generally applicable quorum, and that such quorum may not be less than one-third of the 

outstanding voting shares.  

Resolutions of the general meeting of shareholders are adopted by a simple majority of votes cast without 

quorum requirement, except where Dutch law or our articles of association provide for a special majority 

and/or quorum in relation to specified resolutions.  

 
 
 
Anti-takeover provisions 

We have adopted several provisions that may have the effect of making a takeover of our Company more 

difficult or less attractive, including: 

 

granting a perpetual and repeatedly exercisable call option to a protection foundation, which confers 

upon the protection foundation the right to acquire, under certain conditions, the number of preferred 

 

 

 

shares in the capital of the Company. The issuance of such preferred shares will occur upon the 

protection foundation’s exercise of the call option and will not require shareholder consent; 

the staggered four-year terms of our Supervisory Board members, as a result of which only 

approximately one-fourth of our Supervisory Board members will be subject to election in any one year; 

a provision that our Management Board members and Supervisory Board members may only be 

appointed upon a binding nomination by our Supervisory Board, which can be set aside by a two-thirds 

majority of our shareholders representing more than half of our issued share capital;  

a provision that our Management Board members and Supervisory Board members may only be 

removed by our general meeting of shareholders by a two-thirds majority of votes cast representing 

more than 50% of our issued share capital (unless the removal was proposed by the Supervisory Board); 

and  

 

a requirement that certain matters, including an amendment of our articles of association, may only be 

brought to our shareholders for a vote upon a proposal by our Management Board that has been 

approved by our Supervisory Board.  

Deviations from the Dutch Corporate Governance Code 

The Code contains a “comply-or-explain” principle, offering the possibility to deviate from the Code as long as 

any such deviations are explained. We acknowledge the importance of good corporate governance. However, 

at this stage, we do not comply with all the provisions of the DCGC for specific reasons. The main deviations 

from best practice provisions are listed below. 

 

Pursuant to the best practice provisions 3.1.2.vi and 3.1.2.vii of the DCGC, options granted to our 

Management Board members should not be exercisable during the first three years after the date of 

grant; shares granted to our Management Board members for no financial consideration should be 

retained by them for a period of at least five years or until they cease to hold office, whichever is the 

shorter period; and the number of options and/or shares granted to our management Board members 

should be dependent on the achievement of pre-determined performance criteria. We do not intend to 

comply with all of the above requirements as we believe it is in the best interest of the company to 

attract and retain highly skilled Management Board members on conditions based on market 

competitiveness.  

 

Pursuant to best practice provision 3.2.3 the remuneration of the Management Board in the event of 

dismissal may not exceed one year’s salary. The management services agreements with our 

Management Board members provide for a lump-sum equal to 24 months of the individual’s monthly 

gross fixed salary. Based on the risk profile of the Company and to be able to attract highly skilled 

management, we assumed this period to be appropriate. 

  Best practice provision 3.3.2 prohibits the granting of shares or rights to shares to members of the 

Supervisory Board as compensation. It is common practice for companies listed on the NASDAQ Global 

Market to grant shares to the members of the Supervisory Board as compensation, in order to align the 

interests of the members of the Supervisory Board with our interests and those of our shareholders, and 

we have granted and expect to grant options to acquire ordinary shares to some of our Supervisory 

Board members.  

 

Pursuant to best practice provision 3.3.3, any shares held by Supervisory Board members are long-term 

investments. We do not request our Supervisory Board members to comply with this provision. We 

 
 
 
 
believe it is in the best interest of the Company not to apply this provision in order to be able to attract 

and retain highly skilled Supervisory Board members on internationally competitive terms. 

  Best practice provision 4.3.3 provides that the general meeting of shareholders may pass a resolution to 

cancel the binding nature of a nomination for the appointment of a member of the Management Board 

or of the Supervisory Board or a resolution to dismiss such member by an absolute majority of the votes 

cast. It may be provided that such majority should represent a given proportion of the issued capital, but 

this proportion may not exceed one third. In addition, best practice 4.3.3 provides that if such proportion 

of the share capital is not represented at the meeting, but an absolute majority of the votes cast is in 

favor of a resolution to cancel the binding nature of the nomination, a new general meeting of 

shareholders will be convened where the resolution may be adopted by absolute majority, regardless of 

the proportion of the share capital represented at the meeting. Our articles of association provide that 

these resolutions can only be adopted with at least a 2/3 majority which must represent more than 50% 

of our issued capital, and that no such second meeting will be convened, because we believe that the 

decision to overrule a nomination by the Management Board or the Supervisory Board for the 

appointment or dismissal of a member of our Management Board or of our Supervisory Board must be 

widely supported by our shareholders.  

  Best practice provision 4.2.3 stipulates that meetings with analysts, presentations to analysts, 

presentations to investors and institutional investors and press conferences must be announced in 

advance on the Company’s website and by means of press releases. Provision must be made for all 

shareholders to follow these meetings and presentations in real time, for example by means of 

webcasting or telephone. After the meetings, the presentations must be posted on the Company’s 

website. We believe that enabling shareholders to follow in real time all the meetings with analysts, 

presentations to analysts and presentations to investors, would create an excessive burden on our 

resources and therefore, we do not intend to comply with all of the above requirements.  

  Best practice provision 4.2.2 stipulates that an outline policy on bilateral contacts with the shareholders 

shall be formulated and published on the Company’s website. The Company has not formulated such 

policy as it believes this is already covered by our regular process for public disclosure of information. 

Summary of significant corporate governance differences from NASDAQ Listing Standards 

Our ordinary shares are listed on NASDAQ. The Sarbanes-Oxley Act of 2002, as well as related rules 

subsequently implemented by the SEC, requires foreign private issuers, including our Company, to comply 

with various corporate governance practices. As a foreign private issuer, subject to certain exceptions, the 

NASDAQ listing standards permit a foreign private issuer to follow its home country practice in lieu of the 

NASDAQ listing standards. Our corporate governance practices differ in certain respects from those that U.S. 

companies must adopt in order to maintain a NASDAQ listing. The home country practices followed by our 

Company in lieu of NASDAQ rules are described below:  

  We do not intend to follow NASDAQ’s quorum requirements applicable to meetings of shareholders. In 

accordance with Dutch law and generally accepted business practice, our articles of association do not 

provide quorum requirements generally applicable to general meetings of shareholders.  

  We do not intend to follow NASDAQ’s requirements regarding the provision of proxy statements for 

general meetings of shareholders. Dutch law does not have a regulatory regime for the solicitation of 

proxies and the solicitation of proxies is not a generally accepted business practice in the Netherlands. 

We do intend to provide shareholders with an agenda and other relevant documents for the general 

meeting of shareholders and shareholders will be entitled to give proxies and voting instructions to us 

and/or third parties. 

We intend to take all actions necessary for us to maintain compliance as a foreign private issuer under the 

applicable corporate governance requirements of the Sarbanes-Oxley Act of 2002, the rules adopted by the 

SEC and NASDAQ’s listing standards. 

 
 
 
Controls and procedures 

In accordance with the Dutch Corporate Governance Code, we have assessed the design and operational 

effectiveness of our Risk & Control framework. Based on the activities performed during 2018, and in 

accordance with provision 1.4.3, the Management Board considers that: 

 

 

 

 

this report provides sufficient insights into any failings in the effectiveness of the internal risk 

management and control systems; 

the aforementioned systems provide reasonable assurance that the financial reporting does not contain 

any material inaccuracies; 

based on the current state of affairs, it is justified that the financial reporting is prepared on a going 

concern basis; and 

the report states those material risks and uncertainties that are relevant to the expectation of the 

company’s continuity for the period of twelve months after the preparation of this report. 

In accordance with the Dutch Financial Supervision Act, section 5.25c, the Management Board declares that, 

to the best of its knowledge: 

 

 

the financial statements for 2018 provide, in accordance with IFRS as endorsed by the EU, a true and fair 

view of the consolidated assets, liabilities and financial position as at December 31, 2018, and of the 2018 

consolidated income statement of ProQR Therapeutics N.V.;  

the annual report provides a true and fair view of the situation as at December 31, 2018, and the state of 

affairs during the financial year 2018, together with a description of the principal risks faced by the Group  

 
 
 
 
 
Our business is subject to numerous risks and uncertainties. In the table below, we focus on the key risks and 

uncertainties the Company currently faces. For the avoidance of doubt, this does not mean that the risks 

which were previously signaled and not described here are no longer relevant. For a complete understanding 

of the risks that we face you should also read the full list of risks and uncertainties as disclosed in item 3.D 

Risk Factors of the annual report on Form 20-F. Some of these risks and uncertainties are outside the control 

of the Company, others may be influenced or mitigated. In 2015, we have implemented a Risk & Control 

framework, based on the COSO 2013 internal control framework, for enhancing our control environment as 

well as compliance with the U.S. SEC’s Sarbanes Oxley (SOx) Act of 2002, which we are required to do as a 

company listed on the NASDAQ. As part of the SOx implementation program, our Risk & Control framework 

was further enhanced in 2018, focusing on IT and entity level controls. Improvement of our Risk & Control 

framework is an ongoing effort for the Company. 

We have defined our risk tolerance on a number of internal and external factors including: 

 

 

Financial strength in the long run; 

Liquidity in the short run;  

  Business performance measures;  

 

Scientific risks and opportunities;  

  Compliance with relevant rules and regulations;  
 

Reputation.  

The identification and analysis of risks is an ongoing process that is naturally a critical component of internal 

control. On the basis of these factors and ProQR’s risk tolerance, improvement of our Risk & Control 

framework and monitoring of the risks is an ongoing effort for the Company. 

Our main risks are those that threaten the achievement of the Company’s corporate objectives, including 

compliance. If any of these risks actually occurs, our business, prospects, operating results and financial 

condition could suffer materially. These risks include, but are not limited to, the following:  

Risk related to 

Risk area 

Expected impact upon 
materialization 

Risk appetite /  
risk-mitigating actions 

Development and 
Regulatory Approval of 
our Product Candidates 

Our products might not be able 

The Company would be unable 

This is an inherent risk with drug 

to demonstrate safety and 
efficacy in the preclinical studies 

to commercialize the products 
and therefore generate 

development as the safety and 
efficacy of products can only be 

and clinical trials that are 

revenues. 

needed to obtain product 

approval. 

assessed when these studies 

are conducted. However, the 

Company has multiple products 
in the pipeline and therefore is 

diversified. The Company also 

monitors the progress of the 

programs and aims to make 
decisions that mitigate safety 

and efficacy related risks. 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
Risk related to 

Risk area 

Expected impact upon 
materialization 

Risk-mitigating actions 

The regulatory approval process 
is lengthy, time-consuming and 

Failure to comply with the 
requirements in the regulatory 

Although the Company 
monitors the regulatory 

unpredictable and products 

process could result in delays, 

landscape and engages with the 

developed may ultimately not 

suspension, refusals and 

authorities when it deems that 

lead to regulatory approval of 
the product. 

withdrawal of approvals as well 
as fines. 

necessary, this is an inherent 
risk in biotech drug 

development and therefore has 

limited mitigation abilities. 

We may not able to maintain 
orphan product status for 

We may not benefit from 
rewards including fee reduc-

We take orphan drug 
requirements into consideration 

eluforsen, sepofarsen (formerly: 
QR‑110), QR-411 and QR‑421a 

tions and market exclusivity. 

in the design of our clinical 

Sales could be impacted if other 

development plans. 

or obtain such status for any 
other product canditates. 

products are granted 
authorization for the same 

indications as eluforsen, 

sepofarsen, QR-411 and 
QR‑421a. 

We may be precluded from 
obtaining marketing 

We may encounter delays in 
marketing our products for a 

We take orphan drug 
requirements into consideration 

authorization for our products 

significant period of time. 

in the design of our clinical 

when our competitors have 

obtained market exclusivity 
before we do. 

development plans. 

Capital Needs and 
Financial Position 

The Company depends largely 

Volatility of the Company’s 

The ability of third party 

on equity financing and 

share price, failure to deliver 

financing is dependent on 

Dependence on Third 
Parties 

financing through third party 

under collaboration agreements 

external factors and is therefore 

collaboration agreements and 
government subsidies.  

and/or the reevaluation or 
withdrawal of government 

not entirely in the Company’s 
control. The Company monitors 

subsidies may have a negative 

the market conditions for 

impact on the Company's ability 

opportunities to add additional 

to obtain future financing. 

capital. 

The Company relies upon third-

Failure of third parties to 

The Company reviews and 

party contractors and service 

provide services of a suitable 

monitors the activities of the 

providers for the execution of 

quality and within acceptable 

third parties. These include 

several aspects of its preclinical 
and clinical development 

timeframes may cause delay or 
failure of the Company's 

setting contractual deliverables, 
quality assurance audits and 

programs, which include CRO’s, 

development programs. 

performance reports, among 

third party manufacturers and 

other service providers. 

other activities. 

Intellectual Property 

The Company is highly 

Inadequate intellectual property 

The Company files and 

dependent on its portfolio of 

protection or enforcement may 

prosecutes patent applications 

patents and other intellectual 

impede the Company’s ability to 

to protect its products and 

property, proprietary 
information and knowhow and 

compete effectively. If the 
Company is not able to protect 

technologies to the best of its 
knowledge and with assistance 

its ability to protect and enforce 

its trade secrets, know-how or 

from internal and external 

these assets.  

other proprietary information, 

counsel. Prior to disclosing any 

The Company is subject to the 

the value of its technology and 
product candidates could be 

confidential information to third 
parties, the Company maintains 

risk of infringing third party 

significantly diminished. 

strict confidentiality standards 

intellectual property rights. 

Intellectual property rights 

and agreements for 

collaborating parties.  

conflicts may result in costly 
litigation and could result in the 

Company having to pay 

substantial damages or limit the 

Company’s ability to 
commercialize its product 

candidates. 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Risk related to 

Risk area 

Expected impact upon 
materialization 

Risk-mitigating actions 

Commercialization of Our 
Product Candidates 

We face competition from 
entities that have developed or 

If our competitors develop 
technologies or product 

Competition is an inherent risk 
for any industry including drug 

may develop product 

candidates more rapidly than 

development. Through our IP 

candidates for our target 

we do or their technologies, 

strategy and orphan drug 

indications.  

including delivery technologies, 
are more effective, our ability to 

designation application, we 
attempt to have data exclusivity 

develop and successfully 

for our products. Development 

commercialize our product 

in other companies is essentially 

candidates may be adversely 
affected. 

out of our control but we 
monitor the competitive 

landscape and incorporate that 

into our business strategy. 

Reimbursement from 
third-party payors 

The availability of 
reimbursement by 

If reimbursement is not 
available, or is available only to 

The ability of third party 
financing is dependent on 

governmental and private 

limited levels, we may not be 

external factors and is therefore 

payors is essential for most 

able to successfully 

not entirely in the Company’s 

patients to be able to afford 
expensive treatments. Sales of 

commercialize any product 
candidate. Even if coverage is 

control. The Company monitors 
the market conditions for 

any of our product candidates, if 

provided, the approved 

opportunities to seek 

approved, will depend 

reimbursement amount may 

reimbursement. 

substantially on the extent to 
which the costs of these product 

not be high enough to allow us 
to establish or maintain pricing 

candidates will be paid by health 

sufficient to realize a sufficient 

maintenance, managed care, 

return on our investment. 

pharmacy benefit and similar 
healthcare management 

organizations, or reimbursed by 

government health 

administration authorities, 
private health coverage insurers 

and other third-party payors. 

The above risks have not materialized in 2018. In addition to the above key risks, the Company’s activities 

expose it to a variety of financial risks: market risk (including currency risk, interest rate risk and price risk), 

credit risk and liquidity risk. Unfavorable exchange rate developments and historically low interest rates may 

impact the financial income of the Company. The Company has a cash management policy in place to 

minimize potential adverse effects resulting from unpredictability of financial markets on the Company’s 

financial performance. 

ASSETS 

Non-current assets 

Intangible assets 

Property, plant and equipment 

Current assets 

Social securities and other taxes 

Prepayments and other receivables 

Cash and cash equivalents 

TOTAL ASSETS 

EQUITY 

Share capital 

Share premium 

Reserves 

Accumulated deficit 

Equity attributable to owners of the Company 

Non-controlling interests 

TOTAL EQUITY 

LIABILITIES 

Non-current liabilities 

Borrowings 

Current liabilities 

Borrowings 

Trade payables 

Social securities and other taxes 

Pension premiums 

Deferred income 

Other current liabilities 

Note 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

7 

8 

9 

10 

11 

12 

13 

14 

-- 

1,864 

1,864 

1,243 

1,544 

105,580 

108,367 

39 

2,505 

2,544 

396 

2,064 

48,099 

50,559 

110,231 

53,103 

1,726 

235,744 

10,888 

(155,443) 

92,915 

(230) 

92,685 

1,457 

148,763 

8,513 

(119,370) 

39,363 

(38) 

39,325 

9,386 

9,386 

-- 

135 

-- 

7 

545 

7,473 

8,160 

5,284 

5,284 

1,960 

546 

1,019 

-- 

347 

4,622 

8,494 

TOTAL LIABILITIES 

17,546 

13,778 

TOTAL EQUITY AND LIABILITIES 

110,231 

53,103 

The accompanying notes are an integral part of these financial statements. 

 
 
 
 
 
 
 
 
 
 
 
 
  
  
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
Note 

2018 

€ 1,000 

2017 

€ 1,000 

15 

16 

18 

19 

5,761 

1,495 

(29,514) 

(12,540) 

(31,153) 

(10,840) 

(42,054) 

(41,993) 

(36,293) 

(792) 

(40,498) 

(3,175) 

(37,085) 

(43,673) 

(1) 

(2) 

(37,086) 

(43,675) 

Other income 

Research and development costs 

General and administrative costs 

Total operating costs 

Operating result 

Financial income and expense 

Result before corporate income taxes 

Corporate income taxes 

Result for the year 

Other comprehensive income 

Items that will never be reclassified to profit or loss 

Items that are or may be reclassified to profit or loss 

Foreign operations – foreign currency translation differences 

(28) 

151 

Total comprehensive income for the year 
(attributable to equity holders of the Company) 

Result attributable to 

Owners of the Company 

Non-controlling interests 

(37,114) 

(43,524) 

(36,894) 

(192) 

(37,086) 

(43,637) 

(38) 

(43,675) 

Share information 

20 

Weighted average number of shares outstanding1 

34,052,520 

25,374,807 

Earnings per share attributable to the equity holders  
of the Company (expressed in Euro per share) 

Basic earnings per share1 

Diluted earnings per share1 

The accompanying notes are an integral part of these financial statements.

(1.08) 

(1.08) 

(1.72) 

(1.72) 

1 Basic and diluted earnings are equal due to the anti-dilutive nature of the options outstanding since the Company is loss-
making. 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
  
 
 
 
 
  
 
 
 
 
  
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
                                                             
 
 
Attributable to owners of the Company 

Share 
Capital 

Share 
Premium 

Equity 
Settled 
Employee 
Benefit 
Reserve 

Translation 
Reserve 

Accumulated 
Deficit 

Total 

Non- 
controlling 
Interests 

Total 
Equity 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

Balance at January 1, 2017 

934 

123,597 

4,353 

(15) 

(75,733) 

53,136 

-- 

53,136 

Result for the year 

Other comprehensive income 

Recognition of share-based payments 

Issue of ordinary shares 

Issue of treasury shares 

Share options exercised 

-- 

-- 

-- 

343 

180 

0 

-- 

-- 

-- 

25,342 

(180) 

4 

-- 

-- 

4,024 

-- 

-- 

-- 

-- 

151 

-- 

-- 

-- 

-- 

(43,637) 

(43,637) 

(38) 

(43,675) 

-- 

-- 

-- 

-- 

-- 

151 

4,024 

25,685 

0 

4 

-- 

-- 

-- 

-- 

-- 

151 

4,024 

25,685 

0 

4 

Balance at December 31, 2017 

1,457 

148,763 

8,377 

136 

(119,370) 

39,363 

(38) 

39,325 

Result for the year 

Other comprehensive income 

Recognition of share-based payments 

Issue of ordinary shares 

Share options lapsed 

Share options exercised 

-- 

-- 

4 

265 

-- 

-- 

-- 

-- 

2,185 

83,926 

-- 

870 

-- 

-- 

3,224 

-- 

(97) 

(724) 

-- 

(28) 

-- 

-- 

-- 

-- 

(36,984) 

(36,894) 

(192) 

(37,086) 

-- 

-- 

-- 

97 

724 

(28) 

5,413 

84,191 

-- 

870 

-- 

-- 

-- 

-- 

-- 

(28) 

5,413 

84,191 

-- 

870 

Balance at December 31, 2018 

1,726 

235,744 

10,780 

108 

(155,443) 

92,915 

(230) 

92,685 

The accompanying notes are an integral part of these financial statements.

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
Cash flow from operating activities 

Result for the year 

Adjustments for: 

— Amortization & depreciation 

— Share-based compensation 

— Financial income and expense 

— Net foreign exchange gain / (loss)  

Changes in working capital 

Cash used in operations 

Corporate income tax paid 

Interest received/(paid) 

Note 

2018 

€ 1,000 

2017 

€ 1,000 

(37,086) 

(43,675) 

7, 8   

12 

18 

992 

5,413 

792 

(28) 

1,295 

(28,622) 

(1) 

130 

1,065 

4,024 

3,175 

151 

164 

(35,096) 

(2) 

147 

Net cash used in operating activities 

(28,493) 

(34,951) 

Cash flow from investing activities 

Purchases of intangible assets 

Purchases of property, plant and equipment 

-- 

(312) 

-- 

(121) 

Net cash used in investing activities 

(312) 

(121) 

Cash flow from financing activities 

Proceeds from issuance of shares, net of transaction costs 

84,191 

25,685 

Proceeds from exercise of share options 

Proceeds from borrowings 

Proceeds from convertible loans 

Redemption of financial lease 

Net cash generated by financing activities 

Net increase/(decrease) in cash and cash equivalents 

Currency effect cash and cash equivalents 

Cash and cash equivalents at the beginning of the year 

Cash and cash equivalents at the end of the year 

The accompanying notes are an integral part of these financial statements. 

13 

13 

13 

11 

11 

870 

264 

1,132 

-- 

4 

301 

650 

-- 

86,457 

26,640 

57,652 

(8,432) 

(171) 

48,099 

(2,669) 

59,200 

105,580 

48,099 

 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
  
 
 
 
 
 
  
 
 
 
 
  
 
 
 
 
 
 
 
 
  
 
 
 
 
  
 
 
 
 
 
 
 
 
  
 
 
 
 
  
 
 
 
 
  
 
 
 
 
  
 
 
 
 
ProQR Therapeutics N.V., or “ProQR” or the “Company”, is a development stage company domiciled in the 

Netherlands that primarily focuses on the development and commercialization of novel therapeutic 

medicines.  

Since September 18, 2014, the Company’s ordinary shares are listed on the NASDAQ Global Market under 

ticker symbol PRQR. 

The Company was incorporated in the Netherlands, on February 21, 2012 (Chamber of Commerce no. 

54600790) and was reorganized from a private company with limited liability to a public company with limited 

liability on September 23, 2014. The Company has its statutory seat in Leiden, the Netherlands. The address 

of its headquarters and registered office is Zernikedreef 9, 2333 CK Leiden, the Netherlands. 

At December 31, 2018, ProQR Therapeutics N.V. is the ultimate parent company of the following entities: 

 

 

 

 

 

 

 

 

 

 

ProQR Therapeutics Holding B.V. (the Netherlands, 100%); 

ProQR Therapeutics I B.V. (the Netherlands, 100%); 

ProQR Therapeutics II B.V. (the Netherlands, 100%); 

ProQR Therapeutics III B.V. (the Netherlands, 100%); 

ProQR Therapeutics IV B.V. (the Netherlands, 100%); 

ProQR Therapeutics VI B.V. (the Netherlands, 100%); 

ProQR Therapeutics VII B.V. (the Netherlands, 100%); 

ProQR Therapeutics VIII B.V. (the Netherlands, 100%); 

ProQR Therapeutics IX B.V. (the Netherlands, 100%); 

ProQR Therapeutics I Inc. (United States, 100%); 

  Amylon Therapeutics B.V. (the Netherlands, 80%); 
  Amylon Therapeutics, Inc. (Unites States, a 100% subsidiary of Amylon Therapeutics B.V.) 

ProQR Therapeutics N.V. is also statutory director of Stichting Bewaarneming Aandelen ProQR (“ESOP 

Foundation”) and has full control over this entity.  

As used in these consolidated financial statements, unless the context indicates otherwise, all references to 

“ProQR”, the “Company” or the “Group” refer to ProQR Therapeutics N.V. including its subsidiaries and the 

ESOP Foundation. 

These consolidated financial statements have been prepared in accordance with International Financial 

Reporting Standards, or IFRS, as adopted by the European Union (“EU”). 

With reference to the income statement of the Company, use has been made of the exemption pursuant to 

Section 402 of Book 2 of the Netherlands Civil Code. 

The financial statements have been prepared on the historical cost basis except for financial instruments and 

share-based payment obligations which have been based on fair value. Historical cost is generally based on 

the fair value of the consideration given in exchange for assets.  

 
 
 
 
 
 
These consolidated financial statements are presented in euro, which is the Company’s functional currency. 

All amounts have been rounded to the nearest thousand, unless otherwise indicated. 

The Management Board of ProQR has, upon preparing and finalizing the 2018 financial statements, assessed 

the Company’s ability to fund its operations for a period of at least one year after the date of signing these 

financial statements.  

The Management Board of the Company is confident about the continuity of the Company based on its 

existing funding, taking into account the Company’s current cash position and the projected cash flows based 

on the activities under execution on the basis of ProQR’s business plan and budget. 

In preparing these consolidated financial statements, management has made judgements, estimates and 

assumptions that affect the application of accounting policies and the reported amounts of assets, liabilities, 

income and expenses. Actual results may differ from these estimates.  

Estimates and underlying assumptions are reviewed on an ongoing basis. Revisions to accounting estimates 

are recognized in the period in which the estimate is revised if the revision affects only that period or in the 

period of the revision and future periods if the revision affects both current and future periods.  

Information about assumptions and estimation uncertainties that may have a significant risk of resulting in a 

material adjustment is included below. 

(i) Share-based payments  

Share options granted to employees and consultants are measured at the fair value of the equity instruments 

granted. Fair value is determined through the use of an option-pricing model considering, among others, the 

following variables:  

 

 

 

 

 

 

the exercise price of the option;  

the expected life of the option;  

the current value of the underlying shares;  

the expected volatility of the share price;  

the dividends expected on the shares; and  

the risk-free interest rate for the life of the option.  

For the Company’s share option plans, management’s judgment is that the Black-Scholes valuation method is 

the most appropriate for determining the fair value of the Company’s share options.  

Initially, the Company’s ordinary shares were not publicly traded and consequently the Company needed to 

estimate the fair value of its share and the expected volatility of that value. The expected volatility of all 

options granted was therefore based on the average historical volatility of the Company’s peers over a period 

that agrees with the expected option life. All assumptions and estimates are further discussed in Note 12(d) 

to the financial statements. The value of the underlying shares was determined on the basis of the prior sale 

of company stock method. As such, the Company has benchmarked the value per share to external 

transactions of Company shares and external financing rounds.  

For options granted from the moment of listing as stated above, the Company uses the closing price of the 

ordinary shares on the previous business day as exercise price of the options granted. 

 
 
 
The result of the share option valuations and the related compensation expense is dependent on the model 

and input parameters used. Even though Management considers the fair values reasonable and defensible 

based on the methodologies applied and the information available, others might derive a different fair value 

for the Company’s share options. 

(ii) Corporate income taxes  

The Company recognizes deferred tax assets arising from unused tax losses or tax credits only to the extent 

that the Company has sufficient taxable temporary differences or there is convincing evidence that sufficient 

taxable profit will be available against which the unused tax losses or unused tax credits can be utilized. 

Management’s judgment is that such convincing evidence is currently not sufficiently available and a deferred 

tax asset is therefore only recognized to the extent that the Company has sufficient taxable temporary 

differences.  

(iii) Grant income  

(Government) Grant income is not recognized until there is reasonable assurance that the Company will 

comply with the conditions attached to them. (Government) Grants are recognized in profit or loss on a 

systematic basis over the period the Company recognizes as expenses the related costs for which the grants 

are expected to compensate. 

(iv) Research and development expenditures  

Research expenditures are currently not capitalized but are reflected in the income statement because the 

criteria for capitalization are not met. At each balance sheet date, the Company estimates the level of service 

performed by the vendors and the associated costs incurred for the services performed.  

Although we do not expect the estimates to be materially different from amounts actually incurred, the 

understanding of the status and timing of services performed relative to the actual status and timing of 

services performed may vary and could result in reporting amounts that are too high or too low in any 

particular period.  

The financial statements have been prepared on the basis of International Financial Reporting Standards 

(“IFRS”), as issued by the International Accounting Standards Board (“IASB”).  

IFRS 9  

IFRS 9 contains three principal classification categories for financial assets: measured at amortized cost, 

FVOCI and FVTPL. The classification of financial assets under IFRS 9 is generally based on the business model 

in which a financial asset is managed and its contractual cash flow characteristics. IFRS 9 eliminates the 

previous IAS 39 categories of held to maturity, loans and receivables and available for sale.  

IFRS 9 largely retains the existing requirements in IAS 39 for the classification and measurement of financial 

liabilities. The adoption of IFRS 9 has not had a significant effect on the Group’s accounting policies related to 

financial liabilities and derivative financial instruments.  

Other new Standards and Interpretations, which became effective as of January 1, 2018, did not have a 

material impact on our financial statements.  

 
 
 
 
 
The Company has consistently applied the following accounting policies to all periods presented in these 

consolidated financial statements. 

(i) Subsidiaries 

Subsidiaries are entities controlled by the Group. The Group controls an entity when it has power over the 

entity, is exposed to, or has rights to, variable returns from its involvement with the entity and has the ability 

to affect those returns through its power over the entity. The Group reassesses whether or not it controls an 

entity if facts and circumstances indicate that there are changes to one or more of these elements. The 

financial statements of subsidiaries are included in the consolidated financial statements from the date on 

which control commences until the date on which control ceases. 

(ii) Non-controlling interests (“NCI”) 

NCI are measured at their proportionate share of the acquiree's identifiable net assets at the acquisition 

date. Changes in the Group's interest in a subsidiary that do not result in a loss of control are accounted for 

as equity transactions. 

(iii) Loss of control 

When the Group loses control over a subsidiary, it derecognizes the assets and liabilities of the subsidiary, 

and any non-controlling interests and other components of equity. Any resulting gain or loss is recognized in 

profit or loss. Any interest retained in the former subsidiary is measured at fair value when control is lost.  

(iv) Transactions eliminated on consolidation 

Intra-group balances and transactions, and any unrealized income and expenses arising from intra-group 

transactions, are eliminated. Unrealized gains arising from transactions with equity-accounted investees are 

eliminated against the investment to the extent of the Group’s interest in the investee. Unrealized losses are 

eliminated in the same way as unrealized gains, but only to the extent that there is no evidence of 

impairment. 

Financial instruments are both primary financial instruments, such as receivables and payables, and financial 

derivatives. For primary financial instruments, reference is made to the treatment per the corresponding 

balance sheet item.  

Financial derivatives are valued at fair value. Upon first recognition, financial derivatives are recognized at fair 

value and then revalued as at balance sheet date.  

(i) Foreign currency transactions 

Foreign currency transactions are translated into the functional currency using the exchange rates prevailing 

at the dates of the transactions.  

Monetary assets and liabilities denominated in foreign currencies are translated into the functional currency 

at the exchange rate at the reporting date. Non-monetary assets and liabilities denominated in foreign 

currencies that are measured at fair value are translated into the functional currency at the exchange rate 

when the fair value was determined. Foreign currency differences are generally recognized in profit or loss. 

Non-monetary items that are measured based on historical cost in a foreign currency are not translated. 

 
 
 
 
(ii) Foreign operations

The assets and liabilities of foreign operations are translated into euro at exchange rates at the reporting 

date. The income and expenses of foreign operations are translated into euros at the exchange rates at the 

dates of the transactions. Foreign currency differences are recognized in OCI and accumulated in the 

translation reserve, except to the extent that the translation difference is allocated to NCI. 

Other income includes amounts earned from third parties and are recognized when earned in accordance 

with the substance and under the terms of the related agreements and when it is probable that the economic 

benefits associated with the transaction will flow to the entity and the amount of the income can be 

measured reliably. The grants are recognized in other income on a systematic basis over the period the 

Company recognizes as expenses the related costs for which the grants are expected to compensate. 

The WBSO (“afdrachtvermindering speur- en ontwikkelingswerk”) is a Dutch fiscal facility that provides 

subsidies to companies, knowledge centers and self-employed people who perform research and 

development activities (as defined in the WBSO Act). Under this Act, a contribution is paid towards the labor 

costs of employees directly involved in research and development. The contribution is in the form of a 

reduction of payroll taxes and social security contributions recognized on a net basis within the labor costs. 

Subsidies relating to labor costs are deferred and recognized in the income statement in the period 

necessary to match them with the labor costs that they are intended to compensate.  

(i) Short-term employee benefits

Short-term employee benefits are expensed as the related service is provided. A liability is recognized for the 

amount expected to be paid if the Group has a present legal or constructive obligation to pay this amount as 

a result of past service provided by the employee and the obligation can be estimated reliably. 

(ii) Share-based payment transactions

The grant-date fair value of equity-settled share-based payment awards granted to employees is generally 

recognized as an expense, with a corresponding increase in equity, over the vesting period of the awards. The 

amount recognized as an expense is adjusted to reflect the number of awards for which the related service 

and non-market performance conditions are expected to be met, such that the amount ultimately recognized 

is based on the number of awards that meet the related service conditions at the vesting date. For share-

based payment awards with non-vesting conditions, the grant-date fair value of the share-based payment is 

measured to reflect such conditions and there is no true-up for differences between expected and actual 

outcomes. 

(iii) Pension obligations

The Company operates defined contribution pension plans for all employees funded through payments to 

insurance companies. The Company has no legal or constructive obligation to pay further contributions once 

the contributions have been paid. The contributions are recognized as employee benefit expense when 

employees have rendered the service entitling them to the contributions. Prepaid contributions are 

recognized as an asset to the extent that a cash refund or a reduction in the future payments is available.  

Income tax expense represents the sum of the tax currently payable and deferred tax. It is recognized in 

profit or loss except to the extent that it relates to a business combination, or items recognized directly in 

equity or in OCI. 

(i) Current tax  

The tax currently payable is based on taxable profit for the year. Taxable profit differs from profit as reported 

in the income statement because of items of income or expense that are taxable or deductible in other years 

and items that are never taxable or deductible. The Company’s liability for current tax is calculated using tax 

rates that have been enacted or substantively enacted by the end of the reporting period. 

(ii) Deferred tax  

Deferred tax is recognized on differences between the carrying amounts of assets and liabilities in the 

financial statements and the corresponding tax bases used in the computation of taxable profit.  

The carrying amount of deferred tax assets is reviewed at the end of each reporting period and reduced to 

the extent that it is no longer probable that sufficient taxable profits will be available to allow all or part of the 

asset to be recovered. Since the Company does not expect to be profitable in the foreseeable future, its 

deferred tax assets are valued at nil.  

Deferred tax assets and liabilities are measured at the tax rates that are expected to apply in the period in 

which the liability is settled or the asset realized, based on tax rates (and tax laws) that have been enacted or 

substantively enacted by the end of the reporting period. The measurement of deferred tax liabilities and 

assets reflects the tax consequences that would follow from the manner in which the Company expects, at 

the end of the reporting period, to recover or settle the carrying amount of its assets and liabilities.  

(i) Licenses  

Acquired patents have a finite useful life and are carried at cost less accumulated amortization and 

impairment losses. Amortization is calculated using the straight-line method to allocate the cost of patents 

over their estimated useful lives (generally 10 years unless a patent expires prior to that date). Amortization 

begins when an asset is available for its intended use.  

(ii) Research and development  

Research expenditures are recognized as expenses as incurred. Costs incurred on development projects are 

recognized as intangible assets as of the date that it can be established that it is probable that future 

economic benefits that are attributable to the asset will flow to the Company considering its commercial and 

technological feasibility, generally when filed for regulatory approval for commercial production, and when 

costs can be measured reliably. Given the current stage of the development of the Company’s products no 

development expenditures have yet been capitalized.  

Registration costs for patents are part of the expenditures for the research and development project. 

Therefore, registration costs for patents are expensed as incurred as long as the research and development 

project concerned does not yet meet the criteria for capitalization.  

(iii) Other intangible assets 

Other intangible assets, including software, that are acquired by the Company and have finite useful lives are 

measured at cost less accumulated amortization and accumulated impairment losses. 

(iv) Amortization 

Amortization is calculated to write off the cost of intangible assets less their estimated residual values using 

the straight-line method over their estimated useful lives, and is recognized in profit or loss.  

 
 
 
 
 
The estimated useful lives for current and comparative periods are as follows: 

 

software:  

3 years. 

Amortization methods, useful lives and residual values are reviewed at each reporting date and adjusted if 

appropriate. 

(i) Recognition and measurement 

Items of property, plant and equipment are measured at cost less accumulated depreciation and any 

accumulated impairment losses. If significant parts of an item of property, plant and equipment have 

different useful lives, then they are accounted for as separate items (major components) of property, plant 

and equipment. Any gain or loss on disposal of an item of property, plant and equipment is recognized in 

profit or loss. 

(ii) Depreciation 

Depreciation is calculated to write off the cost of items of property, plant and equipment less their estimated 

residual values using the straight-line method over their estimated useful lives, and is recognized in profit or 

loss. Leased assets are depreciated over the shorter of the lease term and their useful lives unless it is 

reasonably certain that the Company will obtain ownership by the end of the lease term.  

The estimated useful lives of property, plant and equipment for current and comparative periods are as 

follows: 

 

 

 

leasehold improvements:  

5 - 10 years; 

laboratory equipment:  

other:  

5 years; 

3 - 5 years. 

Depreciation methods, useful lives and residual values are reviewed at each reporting date and adjusted if 

appropriate. 

At the end of each reporting period, the Company reviews the carrying amounts of its tangible and intangible 

assets to determine whether there is any indication that those assets have suffered an impairment loss. If 

any such indication exists, the recoverable amount of the asset is estimated in order to determine the extent 

of the impairment loss (if any). Where it is not possible to estimate the recoverable amount of an individual 

asset, the Company estimates the recoverable amount of the cash-generating unit to which the asset 

belongs. Where a reasonable and consistent basis of allocation can be identified, corporate assets are also 

allocated to individual cash-generating units, or otherwise they are allocated to the smallest group of cash-

generating units for which a reasonable and consistent allocation basis can be identified.  

Intangible assets with indefinite useful lives and intangible assets not yet available for use are tested for 

impairment at least annually, and whenever there is an indication that the asset may be impaired.  

The recoverable amount is the higher of fair value less costs of disposal and value in use. In assessing value in 

use, the estimated future cash flows are discounted to their present value using a pre-tax discount rate that 

reflects current market assessments of the time value of money and the risks specific to the asset for which 

the estimates of future cash flows have not been adjusted.  

 
 
 
 
 
 
 
 
 
 
 
 
 
 
If the recoverable amount of an asset (or cash-generating unit) is estimated to be less than its carrying 

amount, the carrying amount of the asset (or cash-generating unit) is reduced to its recoverable amount. An 

impairment loss is recognized immediately in profit or loss, unless the relevant asset is carried at a revalued 

amount, in which case the impairment loss is treated as a revaluation decrease.  

Where an impairment loss subsequently reverses, the carrying amount of the asset (or cash-generating unit) 

is increased to the revised estimate of its recoverable amount, but so that the increased carrying amount 

does not exceed the carrying amount that would have been determined had no impairment loss been 

recognized for the asset (or cash-generating unit) in prior years. A reversal of an impairment loss is 

recognized immediately in profit or loss, unless the relevant asset is carried at a revalued amount, in which 

case the reversal of the impairment loss is treated as a revaluation increase.  

All financial assets are recognized and derecognized on the trade date where the purchase or sale of a 

financial asset is under a contract whose terms require delivery of the financial asset within the timeframe 

established by the market concerned, and are initially measured at fair value and subsequently measured at 

amortized cost or fair value on the basis of the entity’s business model for managing the financial assets and 

the contractual cash flow characteristics of the financial assets. 

Specifically:  

 

 

debt instruments that are held within a business model whose objective is to collect the contractual 

cash flows, and that have contractual cash flows that are solely payments of principal and interest on 

the principal amount outstanding, are measured subsequently at amortised cost, and 

all other debt investments and equity investments are measured subsequently at fair value through 

profit or loss (FVTPL). 

Contract receivables, loans and other receivables that have fixed or determinable payments that are not 

quoted in an active market are classified as “loans and receivables”. Loans and receivables are measured at 

amortized cost using the effective interest method, less any impairment. 

The group applies the IFRS 9 simplified approach to measuring expected credit losses which uses a lifetime 

expected loss allowance for all trade receivables. To measure the expected credit losses, trade receivables 

have been grouped based on shared credit risk characteristics and the days past due. Trade receivables are 

written off when there is no reasonable expectation of recovery. Indicators that there is no reasonable 

expectation of recovery include, amongst others, the failure of a debtor to engage in a repayment plan with 

the group, and a failure to make contractual payments for a period of greater than 120 days past due. 

Impairment losses on trade receivables and contract assets are presented as net impairment losses within 

operating profit. Subsequent recoveries of amounts previously written off are credited against the same line 

item.  

Previous accounting policy for impairment of trade receivables  

In the prior year, an allowance for doubtful accounts is established when there is objective evidence that the 

Company will not be able to collect all amounts due according to the original terms of receivables. Significant 

financial difficulties of the debtor, probability that the debtor will enter into bankruptcy or financial 

reorganization, and default or delinquency in payments are considered indicators that the trade receivable is 

impaired. Loans and receivables are included in ‘current assets’, except for maturities greater than 12 months 

after the balance sheet date, which are classified as ‘non-current assets’. 

 
 
 
Cash and cash equivalents include cash on hand and all highly liquid investments with original maturities of 

three months or less that are convertible to a known amount of cash and bear an insignificant risk of change 

in value.  

Debt and equity instruments are classified as either financial liabilities or as equity in accordance with the 

substance of the contractual arrangement.  

(i) Equity instruments  

An equity instrument is any contract that evidences a residual interest in the assets of an entity after 

deducting all of its liabilities. Equity instruments issued by the Company are recognized at the proceeds 

received, net of direct issue costs.  

(ii) Compound financial instruments 

Compound financial instruments issued by the Group comprise convertible notes denominated in euro that 

can be converted to share capital at the option of the holder, when the number of shares to be issued is fixed 

and does not vary with changes in fair value.  

The liability component of a compound financial instrument is recognized initially at the fair value of a similar 

liability that does not have an equity conversion option. The equity component is recognised initially at the 

difference between the fair value of the compound financial instrument as a whole and the fair value of the 

liability component. Any directly attributable transaction costs are allocated to the liability and equity 

components in proportion to their initial carrying amounts. 

Subsequent to initial recognition, the liability component of a compound financial instrument is measured at 

amortized cost using the effective interest method. The equity component of a compound financial 

instrument is not remeasured. 

Interest related to the financial liability is recognized in profit or loss. On conversion, the financial liability is 

reclassified to equity and no gain or loss is recognized. 

(iii) Other financial liabilities  

Other financial liabilities, including borrowings, are initially measured at fair value, net of transaction costs 

incurred, and are subsequently measured at amortized cost using the effective interest method, with interest 

expense recognized on an effective yield basis.  

The effective interest method is a method of calculating the amortized cost of a financial liability and of 

allocating interest expense over the relevant period. The effective interest rate is the rate that exactly 

discounts estimated future cash payments through the expected life of the financial liability, or, where 

appropriate, a shorter period.  

Borrowings and other financial liabilities are classified as ‘non-current liabilities,’ other than liabilities with 

maturities up to one year, which are classified as “current liabilities”.  

The Company derecognizes financial liabilities when the liability is discharged, cancelled or expired. For all 

financial liabilities, the fair value approximates its carrying amount.  

 
 
 
 
(i) Determining whether an arrangement contains a lease 

At inception of an arrangement, the Company determines whether such an arrangement is or contains  

a lease. 

At inception or on reassessment of an arrangement that contains a lease, the Company separates payments 

and other consideration required by such an arrangement into those for the lease and those for other 

elements on the basis of their relative fair values. If the Company concludes for a finance lease that it is 

impracticable to separate the payments reliably, then an asset and a liability are recognized at an amount 

equal to the fair value of the underlying asset. Subsequently, the liability is reduced as payments are made 

and an imputed finance cost on the liability is recognized using the Company’s incremental borrowing rate. 

(ii) Leased assets 

Assets held by the Company under leases that transfer to the Company substantially all of the risks and 

rewards of ownership are classified as finance leases. The leased assets are measured initially at an amount 

equal to the lower of their fair value and the present value of the minimum lease payments. Subsequent to 

initial recognition, the assets are accounted for in accordance with the accounting policy applicable to  

that asset.  

Assets held under other leases are classified as operating leases and are not recognized in the Company’s 

statement of financial position. 

(iii) Lease payments 

Payments made under operating leases are recognized in profit or loss on a straight-line basis over the term 

of the lease. Lease incentives received are recognized as an integral part of the total lease expense, over the 

term of the lease. 

Minimum lease payments made under finance leases are apportioned between the finance expense and the 

reduction of the outstanding liability. The finance expense is allocated to each period during the lease term 

so as to produce a constant periodic rate of interest on the remaining balance of the liability. 

A number of new standards, amendments to standards and interpretations are effective for annual periods 

beginning after January 1, 2019, and have not been applied in preparing these consolidated financial 

statements. Those which may be relevant to the Group are set out below. The Group does not plan to adopt 

these standards early. 

IFRS 16 Leases 

IFRS 16 specifies how a company will recognize, measure, present and disclose leases. The standard provides 

a single lessee accounting model, requiring lessees to recognize assets and liabilities for all leases unless the 

lease term is 12 months or less or the underlying asset has a low value. Lessors continue to classify leases as 

operating or finance, with IFRS 16’s approach to lessor accounting substantially unchanged from its 

predecessor, IAS 17. 

The impact on the income statement is that current operating expenses will be replaced by depreciation and 

interest. Total expenses (depreciation for 'right of use' assets and interest on lease liabilities) are higher in the 

earlier years of a typical lease and lower in the later years, in comparison with current accounting for 

operating leases. The main impact on the statement of cash flows is higher cash flows from operating 

 
 
 
 
activities, since cash payments for the principal part of the lease liability are classified in the net cash flow 

from financing activities by approximately € 1.2 million. 

IFRS 16 is effective for annual reporting periods beginning on or after January 1, 2019, with early adoption 

permitted and is expected to have an effect on our balance sheet of approximately € 2.3 million. 

There are no other standards that are not yet effective and that would be expected to have a material impact 

on the entity in the current or future reporting periods and on foreseeable future transactions.  

The Company’s activities expose it to a variety of financial risks: market risk (including currency risk, interest 

rate risk and price risk), credit risk and liquidity risk. The Company’s overall financial risk management seeks 

to minimize potential adverse effects resulting from unpredictability of financial markets on the Company’s 

financial performance.  

Financial risk management is carried out by the finance department. The finance department identifies and 

evaluates financial risks and proposes mitigating actions if deemed appropriate.  

(a) Market risk  

Market risk is the risk that changes in market prices – such as foreign exchange rates, interest rates and 

equity prices – will affect the Company’s income or the value of its holdings of financial instruments. The 

objective of market risk management is to manage and control market risk exposures within acceptable 

parameters, while optimizing the return. 

Foreign exchange risk 

Foreign exchange risk arises from future commercial transactions and recognized assets and liabilities in 

foreign currencies, primarily with respect to the U.S. Dollar. The Company has an exposure associated with 

the time delay between entering into a contract, budget or forecast and the realization thereof. The Company 

operates a foreign exchange policy to manage the foreign exchange risk against the functional currency 

based on the Company’s cash balances and the projected future spend per major currency. 

At December 31, 2018 there was a net liability in U.S. Dollars of € 0.1 million (2017: € 0.7 million). Foreign 

currency denominated receivables and trade payables are short term in nature (generally 30 to 45 days). As a 

result foreign exchange rate movements on receivables and trade payables, during the years presented had 

an immaterial effect on the financial statements.  

At year-end, a substantial amount of our cash balances are denominated in U.S. Dollars. This amount reflects 

our current expectation of future expenditure in U.S. dollars. 

A reasonably possible weakening of the U.S. Dollar by 10% against all other currencies at December 31, 2018 

would have affected the measurement of our cash balances denominated in a U.S. Dollar and affected equity 

and profit or loss by € 2.4 million (2017: € 2.5 million). The analysis assumes that all other variables, in 

particular interest rates, remain constant. 

Price risk 

The market prices for the production of preclinical and clinical materials and services as well as external 

contracted research may vary over time. Currently, the commercial prices of any of the Company’s product 

candidates is uncertain. When the development products near the regulatory approval date or potential 

 
 
 
 
 
 
regulatory approval date, the uncertainty of the potential sales price decreases. The Company is not exposed 

to commodity price risk.  

Furthermore the Company does not hold investments designated for sale, therefore are not exposed to 

equity securities price risk.  

Cash flow and fair value Interest rate risk  

The Company’s exposure to interest rate risks is limited due to the use of loans with fixed rates. The 

Company has several loans with fixed interest rates, totaling € 9,386,000 at December 31, 2018 (2017:  

€ 7,244,000). Details on the interest rates and maturities of these loans are provided in Note 13.  

(b) Credit risk  

Credit risk represents the risk of financial loss caused by default of the counterparty. The Company has no 

large receivables balances with external parties. The Company’s principal financial assets are cash and cash 

equivalents which are held at ABN Amro, Rabobank and Wells Fargo. Our cash management policy is focused 

on preserving capital, providing liquidity for operations and optimizing yield while accepting limited risk 

(Short-term credit ratings must be rated A-1/P-1/F1 at a minimum by at least one of the Nationally 

Recognized Statistical Rating Organizations (NRSROs) specifically Moody’s, Standard & Poor’s or Fitch. Long-

term credit rating must be rated A2 or A at a minimum by at least one NRSRO). 

At December 31, 2018 and December 31, 2017, substantially all of our cash and cash equivalents were held at 

three large institutions, Rabobank, ABN Amro and Wells Fargo. All institutions are highly rated (ratings of Aa3, 

A1 and A2 for Rabobank, ABN Amro and Wells Fargo respectively) with sufficient capital adequacy and 

liquidity metrics. 

There are no financial assets past due date or impaired. No credit limits were exceeded during the reporting 

period. 

(c) Liquidity risk  

Liquidity risk represents the risk that an entity will encounter difficulty in meeting obligations associated with 

its financial liabilities. Prudent liquidity risk management implies ensuring sufficient availability of cash 

resources for funding of operations and planning to raise cash if and when needed, either through issue of 

shares or through credit facilities. Management monitors rolling forecasts of the Company’s liquidity reserve 

on the basis of expected cash flow.  

 
 
 
 
 
The table below analyzes ProQR’s undiscounted liabilities into relevant maturity groupings based on the 

remaining period at year-end until the contractual maturity date:  

dsssds  

At December 31, 2018 

Borrowings  

Trade payables and other payables   

At December 31, 2017 

Borrowings  

Trade payables and other payables   

Less than  
1 year 

Between  
1 and 2 years 

Between  
2 and 5 years 

€ 1,000 

€ 1,000 

€ 1,000 

-- 

8,160 

8,160 

797 

-- 

797 

8,984 

-- 

8,984 

Less than  
1 year 

Between  
1 and 2 years 

Between  
2 and 5 years 

€ 1,000 

€ 1,000 

€ 1,000 

1,960 

6,534 

8,494 

980 

-- 

980 

5,981 

-- 

5,981 

Over 
5 years 

€ 1,000 

-- 

-- 

-- 

Over 
5 years 

€ 1,000 

-- 

-- 

-- 

The Company's objectives when managing capital are to safeguard the Company's ability to continue as a 

going concern in order to provide returns for shareholders, benefits for other stakeholders and to maintain 

an optimal capital structure to reduce the cost of capital. 

In order to maintain or adjust the capital structure, the Company may adjust the amount of dividends paid to 

shareholders (although at this time the Company does not have retained earnings and is therefore currently 

unable to pay dividends), return capital to shareholders, issue new shares or sell assets to reduce debt. 

The total amount of equity as recorded on the balance sheet is managed as capital by the Company. 

For financial instruments that are measured on the balance sheet at fair value, IFRS 13 requires disclosure of 

fair value measurements by level of the following fair value measurement hierarchy: 

 

 

 

quoted prices (unadjusted) in active markets for identical assets or liabilities (level 1); 

inputs other than quoted prices included within level 1 that are observable for the asset or liability, either 

directly (that is, as prices) or indirectly (that is, derived from prices) (level 2); and 

inputs for the asset or liability that are not based on observable market data (that is, unobservable 

inputs) (level 3). 

The carrying amount of all financial assets and financial liabilities is a reasonable approximation of the fair 

value and therefore information about the fair values of each class has not been disclosed.  

The Company operates in one reportable segment, which comprises the discovery and development of 

innovative, RNA based therapeutics. The Management Board is identified as the chief operating decision 

maker. The Management Board reviews the operating results regularly to make decisions about resources 

and to assess overall performance.  

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
The Company has not generated any sales revenues since inception. 

All non-current assets of the Company are located in the Netherlands. The amounts provided to the 

Management Board with respect to total assets and liabilities are measured in a manner consistent with that 

of the financial statements. 

dsssds

Balance at January 1, 2017 

Cost 

Accumulated amortization 

Carrying amount 

Additions 

Amortization 

Movement for the period 

Balance at December 31, 2017 

Cost 

Accumulated amortization 

Carrying amount 

Additions 

Impairment charge 

Amortization 

Movement for the period 

Balance at December 31, 2018 

Cost 

Accumulated amortization 

Carrying amount 

Licenses 

Software 

€ 1,000 

€ 1,000 

Total 

€ 1,000 

39 

-- 

39 

-- 

-- 

-- 

39 

-- 

39 

-- 

(39) 

-- 

(39) 

39 

(39) 

-- 

152 

(101) 

51 

-- 

(51) 

(51) 

152 

(152) 

-- 

-- 

-- 

-- 

-- 

152 

(152) 

-- 

191 

(101) 

90 

-- 

(51) 

(51) 

191 

(152) 

39 

-- 

(39) 

-- 

-- 

191 

(191) 

-- 

In 2012, the Company acquired an exclusive license from the Massachusetts General Hospital. The initial 

payment in respect of the license, in the amount of € 39,000 has been impaired and is included in the general 

and administrative costs for an amount of € 39,000 (2017: € -). 

The amortization charge for 2018 is included in the general and administrative costs for an amount of € - 

(2017: € 51,000).  

 
dsssds

Balance at January 1, 2017 

Cost 

Accumulated depreciation 

Carrying amount 

Additions 

Depreciation 

Disposals 

Movement for the period 

Balance at December 31, 2017 

Cost 

Accumulated depreciation 

Carrying amount 

Additions 

Depreciation 

Disposals 

Movement for the period 

Balance at December 31, 2018 

Cost 

Accumulated depreciation 

Carrying amount 

Leasehold 
 improvements 

Laboratory 
 equipment 

Other 

Total 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

1,847 

(508) 

1,339 

9 

(294) 

-- 

(285) 

1,856 

(802) 

1,054 

18 

(296) 

-- 

(278) 

1,874 

(1,098) 

776 

1,957 

(660) 

1,297 

47 

(409) 

-- 

(362) 

2,004 

(1,069) 

935 

281 

(419) 

-- 

(138) 

2,285 

(1,488) 

797 

1,283 

(481) 

802 

26 

(312) 

-- 

(286) 

1,309 

(793) 

516 

13 

(238) 

-- 

(225) 

1,322 

(1,031) 

291 

5,087 

(1,649) 

3,438 

82 

(1,015) 

-- 

(933) 

5,169 

(2,664) 

2,505 

312 

(953) 

-- 

(641) 

5,481 

(3,617) 

1,864 

The depreciation charge for 2018 is included in the research and development costs for an amount of 

€ 725,000 (2017: € 836,000) and in the general and administrative costs for an amount of € 228,000 (2017: 

€ 179,000).  

 
dsssds  

Value added tax 

Wage tax 

All receivables are considered short-term and due within one year.  

dsssds  

Prepayments 

Other receivables 

All receivables are considered short-term and due within one year.  

dsssds  

Cash at banks 

Bank deposits 

The cash at banks is at full disposal of the Company.  

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

311 

932 

1,243 

396 

-- 

396 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

645 

899 

1,544 

1,991 

73 

2,064 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

105,580 

-- 

105,580 

48,099 

-- 

48,099 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
dsssds

Balance at January 1 

Issued for cash 

Issued for services 

Exercise of share options 

Treasury shares issued (transferred) 

Balance at December 31 

Number of ordinary shares 

2018 

2017 

36,425,014 

23,346,856 

6,612,500 

8,573,975 

112,473 

226,098 

-- 

1,034 

(226,098) 

4,503,149 

43,149,987 

36,425,014 

The authorized share capital of the Company amounting to € 7,200,000 consists of 90,000,000 ordinary 

shares and 90,000,000 preference shares with a par value of € 0.04 per share. At December 31, 2018, 

43,149,987 ordinary shares were issued and fully paid in cash, of which 4,277,051 were held by the Company 

as treasury shares (2017: 4,503,149). 

In 2017, the Company has issued 976,477 shares pursuant to its then-current at-the-market offering 

program, resulting in proceeds of € 4,138,000, net of € 127,000 of offering expenses.  

On June 28, 2017, the Company agreed to the issuance of 1,200,000 ordinary shares to institutional investors 

at an issue price of $ 5.00 (€ 4.40) per share in a registered direct offering with gross proceeds of € 5,278,000. 

The closing of the offering was effected on July 3, 2017. Transaction costs amounted to € 414,000, resulting in 

net proceeds of € 4,864,000. 

In November 2017, the Company consummated an underwritten public offering and concurrent registered 

direct offering of 6,397,498 ordinary shares at an issue price of $ 3.25 (€ 2.76) per share. The gross proceeds 

from both offerings amounted to € 17,671,000 while the transaction costs amounted to € 988,000, resulting 

in net proceeds of € 16,683,000. 

In September 2018, the Company consummated an underwritten public offering and concurrent registered 

direct offering of 6,612,500 ordinary shares at an issue price of $ 15.75 per share. The gross proceeds from 

this offering amounted to € 89,983,000 while the transaction costs amounted to € 5,792,000, resulting in net 

proceeds of € 84,191,000. 

In November 2018, the Company issued 112,473 shares in the aggregate amount of $ 2.5 million, at $ 22.23 

(€ 19.46) per share to Ionis Pharmaceuticals, Inc. Under the terms of the agreement, an upfront payment in 

ordinary shares to its common stock was made to Ionis upon signing the worldwide license agreement. The 

Company was granted an exclusive worldwide license to QR-1123 and relevant patents. The Company will 

also make future milestone payments, certain of which will be made in equity and others in cash or equity at 

the company’s discretion, and royalties on net sales of 20% through the royalty term. 

On November 7, 2018, the Company filed a shelf registration statement, which permitted: (a) the offering, 

issuance and sale by the Company of up to a maximum aggregate offering price of $ 300,000,000 of its 

ordinary shares, warrants and/or units; and (b) as part of the $ 300,000,000, the offering, issuance and sale by 

 
us of up to a maximum aggregate offering price of $ 75,000,000 of its ordinary shares that may be issued and 

sold under a sales agreement with H.C. Wainwright & Co in one or more at-the-market offerings.  

The costs of share options for employees, members of the Supervisory Board and members of the 

Management Board are recognized in the income statement, together with a corresponding increase in 

equity during the vesting period, taking into account (deferral of) corporate income taxes. The accumulated 

expense of share options recognized in the income statement is shown separately in the equity category 

‘equity settled employee benefit reserve’ in the ‘statement of changes in equity’. On September 25, 2017, we 

established a Dutch foundation named Stichting Bewaarneming Aandelen ProQR for holding shares in trust 

for employees, members of the Management Board and members of the Supervisory Board of the Company 

and its group companies who from time to time will exercise options under the Company’s equity incentive 

plans. 

The translation reserve comprises all foreign currency differences arising from the translation of the financial 

statements of foreign operations.  

The Company operates an equity-settled share-based compensation plan which was introduced in 2013. 

Options may be granted to employees, members of the Supervisory Board, members of the Management 

Board and consultants. The compensation expenses included in operating costs for this plan were 

€ 3,224,000 in 2018 (2017: € 4,024,000), of which € 2,167,000 (2017: € 2,059,000) was recorded in general and 

administrative costs and € 1,057,000 (2017: € 1,965,000) was recorded in research and development costs 

based on employee allocation.  

Options granted under this stock option plan are exercisable once vested. Any vesting schedule may be 

attached to the granted options, however the typical vesting period is four years (25% after every year). The 

options expire ten years after date of grant. Options granted under the stock option plan are granted at 

exercise prices which equal the fair value of the ordinary shares of the Company at the date of the grant.  

The Company accounts for its employee stock options under the fair value method. The fair value of the 

options is estimated at the date of grant using the Black-Scholes option-pricing model, with on average the 

following assumptions:  

dsssds  

Risk-free interest rate 

Expected dividend yield 

Expected volatility 

Expected life in years 

Options  
granted in 2018 

Options  
granted in 2017 

2.223% 

0% 

80.9% 

5 years 

1.913% 

0% 

88.7% 

5 years 

The resulting weighted average grant date fair value of the options amounted to € 2.02 in 2018 (2017: € 3.21). 

The stock options granted have a 10 year life following the grant date and are assumed to be exercised five 

years from date of grant for all awards. 

 
 
 
 
 
 
 
 
 
 
Movements in the number of options outstanding and their related weighted average exercise prices are as 

follows:  

dsssds  

Balance at January 1 

Granted 

Forfeited 

Exercised 

Expired 

Balance at December 31 

2018 

2017 

Number of 
 options 

Average  
exercise price 

Number of 
 options 

Average  
exercise price 

3,331,875 

1,570,366 

(142,467) 

(226,098) 

(22,164) 

4,511,512 

€ 4.78 

€ 3.11 

€ 4.29 

€ 4.02 

€ 6.42 

€ 4.24 

2,205,989 

1,199,447 

(72,527) 

(1,034) 

-- 

3,331,875 

€ 4.88 

€ 4.63 

€ 5.56 

€ 3.54 

-- 

€ 4.78 

Exercisable 

1,683,731 

1,148,893 

The options outstanding at December 31, 2018 had an exercise price in the range of € 1.11 to € 20.34 (2017: 

€ 1.11 to € 20.34) and a weighted-average contractual life of 7.6 years (2017: 7.9 years). 

The weighted-average share price at the date of exercise for share options exercised in 2018 was € 15.36 

(2017: € 4.23). 

Please refer to Note 23 for the options granted to key management personnel. 

dsssds  

Innovation credit 

Convertible loans 

Accrued interest 

Total borrowings 

Current portion 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

5,164 

1,871 

2,351 

4,899 

662 

1,683 

9,386 

7,244 

-- 

9,386 

(1,960) 

5,284 

Innovation credit (“Innovatiekrediet”)  

On June 1, 2012, ProQR was awarded an Innovation credit by the Dutch government, through its agency RVO 

of the Ministry of Economic Affairs, for the Company’s cystic fibrosis program. Amounts were drawn under 

this facility in the course of the years 2013 through 2017. The credit covers 35% of the costs incurred in 

respect of the program up to € 5.0 million.  

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
The credit is interest-bearing at a rate of 10% per annum. Early October 2018 ProQR received a conditional 
waiver of the € 5 million Innovation credit. The total loan of € 7.4 million including interest will be waived after 
3 years if certain conditions are met. The conditions are reviewed by RVO on an annual basis. 

On December 10, 2018 ProQR was awarded an Innovation credit by the Dutch government, through its 

agency RVO of the Ministry of Economic Affairs, for the QR-110 program. Amounts will be drawn under this 

facility from 2018 through 2021. The credit of € 4.7 million through December 31, 2021 will be used to 

conduct the Phase 2/3 clinical study and efforts to obtain regulatory and ethical market approval (NDA/MAA) 

of QR-110 for LCA10, of which € 163,000 has been received in 2018. The credit, including accrued interest of 

10% per annum, is repayable depending on obtaining market approval. The credit covers 35% of the costs 

incurred in respect of the program up to € 4.7 million. 

The assets which are co-financed with the granted innovation credits are subject to a right of pledge for the 

benefit of RVO. 

Convertible loans 

Convertible loans were issued to Amylon Therapeutics B.V. in 2017 and 2018 and are interest-bearing at an 

average rate of 8% per annum. They are convertible into a variable number of ordinary shares within 36 

months at the option of the holder or the Company in case financing criteria are met. Any unconverted loans 

become payable on demand after 24 -36 months in equal quarterly terms. 

In March 2018, we entered into a convertible loan (the “Loan”), pursuant to which we borrowed an aggregate 

of € 260,000 from the lender. The loan bears interest at a rate of 3% per annum. The outstanding principal 

and interest under the Loan is convertible into our ordinary shares upon the first to occur of the following 

events, at the election of the lender for (i) or (ii): (i) our public announcement of a strategic business 

partnership aimed at joint development of, or development by the partner of, our Huntington’s disease 

program, in which case the lender may convert the outstanding Loan amount into our ordinary shares at the 

then-prevailing trading price less a 25% discount; (ii) our public announcement of our decision to 

independently develop our Huntington’s disease program in the future, in which case the lender may convert 

the outstanding Loan amount into our ordinary shares at the then-prevailing trading price; or, (iii) on or 

around March 30, 2020 at the then-prevailing trading price plus a 25% premium. In no event are we required, 

nor are we permitted, to issue ordinary shares in an amount that exceeds 0.5% of the total number of 

ordinary shares outstanding immediately prior to the entry into the Loan. The Loan agreement restricts the 

lender’s ability to transfer the Loan, and prohibits the lender from entering into or engaging in any hedge, 

swap, short sale, derivative transaction or other agreement or arrangement that transfers any ownership of, 

or interests in, the Loan or our ordinary shares issued or issuable upon conversion of the Loan. The Loan and 

the ordinary shares issuable upon conversion of the Loan were issued in reliance on a private placement 

exemption from registration under the Securities Act of 1933, as amended. 

 
 
 
 
dsssds  

Borrowings 

Trade payables 

Social securities and other taxes 

Pension premiums 

Deferred income 

Accrued expenses and other liabilities 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

-- 

135 

-- 

7 

545 

7,473 

8,160 

1,960 

546 

1,019 

-- 

347 

4,622 

8,494 

At December 31, 2018, current liabilities included deferred income resulting from funds received for one of 

our research and innovation programs. 

dsssds  

Grant income 

Rental income from property subleases 

Other income 

2018 

€ 1,000 

5,378 

174 

209 

5,761 

2017 

€ 1,000 

870 

625 

-- 

1,495 

Other income is incidental by nature. In 2015, the European Commission (EC) through its Horizon 2020 

program awarded us and our academic partners a grant of € 6 million to support the clinical development of 

eluforsen (ProQR: € 4.6 million). Horizon 2020 is one of the largest research and innovation programs in the 

European Union with nearly € 80 billion in available funding for qualified projects from 2014 to 2020. This 

program has ended at December 31, 2017 and the final amount of € 1.3 million has been recognized as other 

income in 2018. 

On February 9, 2018, the Company entered into a partnership agreement with Foundation Fighting Blindness 

(FFB), under which FFB has agreed to provide funding of $ 7.5 million for the preclinical and clinical 

development of QR-421a for Usher syndrome type 2A targeting mutations in exon 13. In 2018 € 2.5 million 

has been recognized as other income. 

On June 5, 2018, the Company entered into a partnership agreement with EB Research Partnership (EBRP) 

and EB Medical Research Foundation (EBMRF) under which EBRP and EBMRF have agreed to provide funding 

of $ 5.0 million for the clinical development of QR-313 for Dystrophic Epidermolysis Bullosa targeting 

mutations in exon 73. In 2018 € 1.3 million has been recognized as other income. 

Grants are recognized in other income in the same period in which the related R&D costs are recognized. 

Research and development costs amounted to € 29,514,000 in 2018 (2017: € 31,153,000) and comprise 

allocated employee costs, the costs of materials and laboratory consumables, the costs of external studies 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
including, amongst others, clinical studies and toxicology studies and external research, license- and IP-costs 

and allocated other costs. 

dsssds

Wages and salaries 

Social security costs 

Pension costs – defined contribution plans 

Equity-settled share based payments 

2018 

€ 1,000 

11,558 

1,346 

868 

3,224 

16,996 

2017 

€ 1,000 

11,855 

1,285 

860 

4,024 

18,024 

Average number of employees for the period 

127.7 

139.9 

Employees per activity at December 31 (converted to FTE): 

dsssds

Research and Development 

General and Administrative 

December 31, 
2018 

December 31, 
2017 

89.2 

29.6 

118.8 

96.2 

34.0 

130.2 

Of all employees 112.8 FTE are employed in the Netherlands (2017: 125.2 FTE). 

Included in the wages and salaries for 2018 is a credit of € 1,294,000 (2017: € 723,000) with respect to WBSO 

subsidies.  

dsssds

Interest income 

Current accounts and deposits 

Interest costs 

Interest on loans and borrowings 

Foreign exchange result 

Net foreign exchange benefit/(loss) 

2018 

€ 1,000 

2017 

€ 1,000 

189 

90 

(810) 

(596) 

(171) 

(2,669) 

(792) 

3,175 

 
 
 
The calculation of the tax charge is as follows: 

dsssds

2018 

€ 1,000 

2017 

€ 1,000 

Income tax provision based on domestic rate 

9,106 

10,918 

Tax effect of: 

Non-deductible expenses 

Deductible expenses 

Tax incentives 

Current year losses for which no deferred tax asset was recognized 

Change in unrecognized deductible temporary differences 

Income tax charge 

Effective tax rate 

(818)- 

1,448 

-- 

(9,710) 

(25) 

1 

0% 

(634) 

-- 

-- 

(10,257) 

(25) 

2 

0% 

Due to the operating losses incurred since inception the Company has no tax provisions as of the balance 

sheet date. Furthermore, no significant temporary differences exist between accounting and tax results.  

Realization of deferred tax assets is dependent on future earnings, if any, the timing and amount of which are 

uncertain. Accordingly, the Company has not yet recognized any deferred tax asset related to operating 

losses. As per December 31, 2018, the Company has a total amount of € 162.6 million (2017: € 123.9 million) 

tax loss carry-forwards available for offset against future taxable profits. According to current tax regulations 

the first amount of the tax loss carry-forwards will expire in 2021.  

Basic earnings per share are calculated by dividing the result attributable to equity holders of the Company 

by the weighted average number of shares outstanding during the year.  

dsssds

Result attributable to equity holders of the Company (€ 1,000) 

Weighted average number of shares  outstanding 

Basic (and diluted) earnings per share (€ per share) 

2018 

2017 

(36,894) 

(43,637) 

34,052,520 

25,374,807 

(1.08) 

(1.72) 

For the periods included in these financial statements, the share options are not included in the diluted 

earnings per share calculation as the Company was loss-making in all periods. Due to the anti-dilutive nature 

of the outstanding options, basic and diluted earnings per share are equal.  

The Company did not declare dividends for any of the years presented in these financial statements. 

 
 
Since 2012, the Company is domiciled in Leiden, the Netherlands, where it currently has entered into rental 

agreements for laboratory space and offices and since late 2018 in Cambridge, Massachusetts, USA.  

We lease facilities of approximately 2,960 square meters in total, located at Zernikedreef in Leiden, the 

Netherlands, where our headquarters and our laboratories are located. The lease for this facility terminates 

on December 31, 2020, and subject to the provisions of the lease, may then be renewed for subsequent 

5 year terms. In May 2018, we entered into an agreement to lease additional office space in the US, at CIC 

Cambridge. Per January 2019, we rent an office of approximately 60 square meters, located at 245 Main 

Street, Cambridge, MA 02142. We believe that our existing facilities are adequate to meet current needs and 

that suitable alternative spaces will be available in the future on commercially reasonable terms. 

The lease expenditure charged to the income statement in 2018 amounts to € 1,813,000 (2017: € 2,103,000). 

The future aggregate minimum lease payments under non-cancellable operating leases are as follows: 

dsssds

Less than 1 year 

Between 1 and 5 years 

More than 5 years 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

1,233 

1,233 

-- 

2,466 

1,607 

3,312 

-- 

4,919 

The Company leased out a part of its office in the U.S. and the Netherlands during 2017 and early 2018. In 

2018, total sublease income amounted to € 174,000 (2017: € 625,000), which is recorded in other income. At 

31 December, the future minimum lease payments under non-cancellable leases are receivable as follows: 

dsssds

Less than 1 year 

Between 1 and 5 years 

More than 5 years 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

-- 

-- 

-- 

-- 

€ 1,000 

174 

-- 

-- 

174 

There are no claims known to management related to the activities of the Company. 

On October 26, 2018, we and Ionis Pharmaceuticals, Inc. entered into a License Agreement, pursuant to 

which Ionis granted an exclusive, worldwide, royalty-bearing license to us to develop and commercialize 

certain pharmaceutical products, including the product designated by Ionis as IONIS-RHO-2.5Rx, which has 

been re-designated by us as QR-1123, for the prevention or treatment of retinitis pigmentosa in humans, 

including patient screening. Ionis also granted to the Company certain sub-license rights. Under the License 

 
 
Agreement, we are required to make an upfront payment of an aggregate of up to $ 6.0 million in 

installments, and certain payments up to an aggregate of $ 20.0 million upon the satisfaction of certain 

development and sales milestones. In addition, Ionis is entitled to royalty payments in the double digits of 

aggregate annual net sales, subject to minimum sales in certain circumstances, and subject to reduced rates 

in certain circumstances. The royalty term lasts on a product-by-product and country-by-country basis, until 

the later of the expiration of the patent rights licensed to us and the expiration of regulatory-based exclusivity 

for such product in such country. The License Agreement may also be terminated by either party based upon 

certain uncured material breach by, or insolvency of, the other party, or by us at any time with advanced 

notice. In connection with the upfront payments and development milestone payments, we also 

simultaneously entered into a Stock Purchase Agreement with Ionis, pursuant to which we agreed to issue an 

aggregate of $ 2.5 million of ordinary shares to satisfy the first installment upfront payment, and the 

remaining installment of the upfront payment in ordinary shares determined upon the due date of such 

installment.  In addition, the Stock Purchase Agreement provides for the ability for us, at our discretion, to 

pay the development milestone payments in ordinary shares when such payments are due.  We may not 

issue ordinary shares to Ionis to the extent that such issuance would result in Ionis owning in excess of 18.5% 

of our issued and outstanding shares, nor may we issue ordinary shares if such issuance, together with 

previous issuances under the Stock Purchase Agreement, would exceed 19.9% of our outstanding ordinary 

shares as of the date of the execution of the Stock Purchase Agreement. Under these circumstances, we are 

required to pay the remainder of the upfront and/or development milestone payments in cash. In addition, in 

connection with the Stock Purchase Agreement, we also entered into an Investor Agreement with Ionis, 

pursuant to which we agreed to register for resale the ordinary shares issued by us under the Stock Purchase 

Agreement, under the circumstances described in the Investor Agreement. The Investor Agreement also 

contains customary covenants related to our registration of such shares, preparation of filings in connection 

therewith and indemnification of Ionis. The Investor Agreement also contains lockup provisions prohibiting 

the disposition of our ordinary shares issued under the Stock Purchase Agreement for a period of 12 months 

from the applicable issuance date, as well as voting provisions requiring Ionis to vote its ordinary shares in 

accordance with the recommendations of our board of directors, in each case subject to certain exceptions  

In April 2014 the Company entered into a Patent License Agreement with Radboud University Medical Center, 

or Radboud in the field of antisense oligonucleotide-based therapy for Leber’s Congenital Amaurosis, or LCA. 

Under the terms of this license agreement, the Company has an exclusive, sublicensable, world-wide royalty-

bearing license under certain Radboud patent rights to develop, make, have made, use, sell offer for sale and 

import of certain licensed products of Radboud for use in all prophylactic and therapeutic uses in the field of 

LCA.  Pursuant to the terms of the license agreement, the Company is obligated to pay Radboud net-sales-

related royalties which shall be determined on a product-by-product and country-by-country basis.  If the 

Company required to pay any third party royalties, it may deduct that amount from that which is owed to 

Radboud. Radboud shall provide human resources, materials, facilities and equipment that are necessary for 

preclinical and clinical trials and if the Company does not purchase such trial facilities from Radboud, it is 

required to pay an increased net-sales-related royalty. In the Company’s sole discretion, it may elect to 

convert the obligation to pay net-sales-related royalties into one of the two lump-sum royalty options 

depending on whether the Company elects to convert prior to or after regulatory approval has been filed. 

The license agreement will remain in effect until the date on which all patent applications and all granted 

patents ensuing from such applications have expired or is terminated earlier in accordance with the 

agreement. Either party may terminate the agreement if the other party is in default of a material obligation 

under the agreement which has not been cured within 30 days of notice of such default.  Either party may 

also terminate the agreement if the other party declares bankruptcy, dissolves, liquidates or the like.  

Radboud may also terminate the agreement if the Company does not pay any amount owed under the 

agreement and such payment remains overdue for at least 30 days after receiving notice from Radboud of 

the amount due. 

In June 2015, we entered into another license agreement with Radboud. Under the terms of this license 

agreement, the Company has an exclusive, sublicensable, world-wide royalty-bearing license under certain 

Radboud patent rights to develop, make, have made, use, sell offer for sale and import of certain licensed 

products of Radboud for use in all prophylactic and therapeutic uses in the field of Usher syndrome.  

Pursuant to the terms of the license agreement, the Company is obligated to pay Radboud net-sales-related 

royalties which shall be determined on a product-by-product and country-by-country basis. If the Company is 

required to pay any third party royalties, it may deduct that amount from that which is owed to Radboud. 

Radboud shall provide human resources, materials, facilities and equipment that are necessary for preclinical 

and clinical trials and if the Company does not purchase such trial facilities from Radboud, it is required to 

pay an increased net-sales-related royalty. In the Company’s sole discretion, it may elect to convert the 

obligation to pay net-sales-related royalties into one of the two lump-sum royalty options depending on 

whether it elects to convert prior to or after regulatory approval has been filed. The license agreement will 

remain in effect until the date on which all patent applications and all granted patents ensuing from such 

applications have expired or is terminated earlier in accordance with the agreement. Either party may 

terminate the agreement if the other party is in default of a material obligation under the agreement which 

has not been cured within 30 days of notice of such default. Either party may also terminate the agreement if 

the other party declares bankruptcy, dissolves, liquidates or the like. Radboud may also terminate the 

agreement if the Company does not pay any amount owed under the agreement and such payment remains 

overdue for at least 30 days after receiving notice from Radboud of the amount due. 

In January 2018, the Company entered into a license agreement with Inserm Transfert SA and Assistance-

Publique-Hôpiteaux de Paris. Under the terms of the agreement, the Company has a world-wide, exclusive, 

royalty-bearing license under patent rights belonging to Inserm Transfert SA and other co-owners to develop, 

have developed, make, have made, use, have used and sell, have sold or otherwise distribute certain licensed 

products related to antisense oligonucleotides for treating LCA and method of treatment claims relating to 

modulation of the splicing of the CEP290 gene product. The Company has the right to grant sublicenses to 

third parties subject to certain limitations such as the sublicensee’s activities do not conflict with the public 

order or ethical obligations of Inserm Transfert SA or any co-owner and do not tarnish the image of Inserm 

Transfert SA or any co-owner. In partial consideration of the rights and licenses granted by the license 

agreement, the Company is required to make payments upon the completion of certain milestones: 

completion of a clinical trial more advanced than First in Man, such as a phase IIb; and the first marketing 

authorization or any foreign equivalent for a first product.  In further consideration of the rights and license 

granted under the agreement, the Company shall pay to Inserm Transfert SA a running royalty on net sales of 

products sold by us or our sublicensee. Unless terminated earlier pursuant to termination provisions of 

Agreement, the license agreement will remain in effect on a country-by-country basis, until the later to occur 

of the following events (i) the invalidation or expiration of the last to expire or to be invalidated patent rights 

which covers the manufacture, use or sale of the product in said country or until the expiration of the 

exclusive commercialization right granted by a regulatory agency to a product as an orphan drug or (ii) five 

years after the first commercial sale of a product in the country in which the product is sold.  The agreement 

may be terminated by either party in the event of an uncured breach by the non-breaching party.  Inserm 

Transfert SA may terminate the agreement if we become the subject of voluntary or involuntary winding-up 

proceedings or judicial recovery, if the Company or its sublicensees interrupt development activities for at 

least one year, if the Company or its sublicensees interrupt commercialization for more than twelve months 

after the first commercialization in a country, if the Company does not commercialize a product within two 

years following our obtaining of marketing approval in a country, or if the Company or our sublicensees do 

not put a product into commercial use and do not keep products reasonably available to the public within 

twelve years of the effective date of the agreement. 

In January 2016, the Company entered into an agreement with Leiden University Medical Center, or LUMC, 

which gives us a world-wide, exclusive, royalty-bearing license in the field of amyloid beta related diseases, 

notably Alzheimer’s disease and HCHWA-D, under certain patent rights of LUMC regarding antisense 

oligonucleotide based therapies. This license agreement contains certain diligence obligations for the 

Company coupled to milestone payments and complements the Company’s intellectual property relating to 

its CNS program. On September 12, 2017, this program was transferred to Amylon Therapeutics B.V., in which 

the Company maintains a majority ownership. 

In January 2017, the Company entered into an agreement with LUMC, which gives us a world-wide, exclusive, 

royalty-bearing license in the field of Huntington’s disease, under certain patent rights of LUMC regarding 

antisense oligonucleotide based therapies. This license agreement contains certain diligence obligations for 

the Company coupled to milestone payments and complements the Company’s intellectual property relating 

to the HD program. 

In 2012, the Company and the General Hospital Corporation (MGH) have entered into a Patent License 

Agreement for the Company’s CF program pursuant to which the Company may have certain royalty and 

milestone obligations. The Company is also obligated to pay MGH up to $ 700,000 (€ 611,000) in milestone 

payments upon the achievement of certain development and regulatory milestones and, beginning after its 

first commercial sale of a product covered by the licensed patent rights, a $ 10,000 (€ 9,000) annual license 

fee which is creditable against royalties due to MGH in the same calendar year. In addition, the Company is 

obligated to pay MGH 2% of any net sales by the Company, its affiliates or sublicensees on licensed products 

made or sold in the United States, as well as a low double-digit percentage of any payments the Company 

may receive from any sublicensee anywhere in the world. 

In August 2014, the Company entered into an agreement with Cystic Fibrosis Foundation Therapeutics, Inc., 

or CFFT, a subsidiary of the Cystic Fibrosis Foundation, pursuant to which CFFT agreed to provide the 

Company with up to $ 3 million (€ 2.6 million) to support the clinical development of eluforsen. 

Pursuant to the terms of the agreement, the Company is obligated to make a one-time milestone payment to 

CFFT of up to approximately $ 16 million (€ 14 million), payable in three equal annual installments following 

the first commercial sale of eluforsen, the first of which is due within 90 days following the first commercial 

sale. The Company is also obligated to make a one-time milestone payment to CFFT of up to $ 3 million (€ 2.6 

million) if net sales of eluforsen exceed $ 500 million (€ 437 million) in a calendar year. Lastly, the Company is 

obligated to make a payment to CFFT of up to approximately $ 6 million (€ 5 million) if it transfers, sells or 

licenses eluforsen other than for certain clinical or development purposes, or if the Company enters into a 

change of control transaction prior to commercialization. However, the payment in the previous sentence 

may be set-off against the $ 16 million milestone payment. Either CFFT or the Company may terminate the 

agreement for cause, which includes the Company’s material failure to achieve certain commercialization and 

development milestones. The Company’s payment obligations survive the termination of the agreement. 

On February 9, 2018, the Company entered into an agreement with Foundation Fighting Blindness (FFB), 
under which FFB will provide funding of $ 7.5 million (€ 6.6 million) to advance QR‑421a into the clinic and will 

receive future milestone payments.  

Pursuant to the terms of the agreement, the Company is obligated to make a one-time milestone payment to 

FFB of up to approximately $ 37.5 million (€ 32.8 million), payable in four equal annual installments following 

the first commercial sale of QR‑421a, the first of which is due within 60 days following the first commercial 

sale. The Company is also obligated to make a payment to FFB of up to approximately $ 15 million (€ 13.1 
million) if it transfers, sells or licenses QR‑421a other than for certain clinical or development purposes, or if 

the Company enters into a change of control transaction. However, the payment in the previous sentence 

may be set-off against the $ 37.5 million milestone payment. Either FFB or the Company may terminate the 

agreement for cause, which includes the Company’s material failure to achieve certain commercialization and 

development milestones. The Company’s payment obligations survive the termination of the agreement. 

On June 5, 2018, the Company entered into a partnership agreement with EB Research Partnership (EBRP) 

and EB Medical Research Foundation (EBMRF) under which EBRP and EBMRF have agreed to provide funding 

of $ 5.0 million for the clinical development of QR-313 for Dystrophic Epidermolysis Bullosa targeting 

mutations in exon 73. 

Pursuant to the terms of the agreement, the Company shall, during the period starting upon completing a 

Successful Clinical Study, pay EBRP the total Actual Award in 12 equal semi-annual installments of the Actual 

Award made by EBRP. ProQR shall have the option, at its discretion, to pay the amount for each installment in 

ProQR Therapeutics N.V. shares at the price of market close on the payment due date. In addition to the 

payment in subparagraph (a) above, ProQR shall pay a return on investment to EBRP in an amount equal of 

1.36 times the Actual Award at the first anniversary of First Commercial Sale; 1.36 times the Actual Award at 

the second anniversary of First Commercial Sale; 1.36 times the Actual Award at the third anniversary of First 

Commercial Sale; and 1.0 times the Actual Award when aggregate Net Sales of the Product exceed $ 100 

million. 

In the event of a License Transaction by ProQR or a Change of Control Transaction (collectively a “Disposition 

Transaction”): (i) ProQR shall pay to EBRP 33.3 percent of any consideration received by ProQR in connection 

with such transaction (whether up front or in subsequent milestone or other payments and whether in cash 

or other property) not to exceed four (4) times the amount of the Actual Award (the “Disposition Payment”), 

provided that (i) the amount of the actual total payment previously made by ProQR under Section 2(a) shall 

be deducted  from the amount of the “Disposition Payment”. 

The Company has research and development commitments, mainly with CRO's, amounting to € 8,114,000 
at December 31, 2018 (2017: € 7,704,000). Of these obligations an amount of € 5,807,000 is due in 2019, 
the remainder is due in 1 to 5 years.

Details of transactions between the Company and related parties are disclosed below.  

The remuneration of the Supervisory Board members in 2018 is set out in the table below: 

dsssds

2018 

Mr. Dinko Valerio 

Mr. Antoine Papiernik 

Ms. Alison Lawton  

Mr. Paul Baart 

Mr. James Shannon 

Short term 
employee 
benefits 

Post 
employment 
benefits 

Share-based 
payment 

Total 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

36 

72 

31 

80 

33 

252 

-- 

-- 

-- 

-- 

-- 

-- 

69 

-- 

75 

-- 

73 

217 

105 

72 

106 

80 

106 

469 

 
The remuneration of the Supervisory Board members in 2017 is set out in the table below:  

dsssds  

2017 

Short term 
employee 
benefits 

Post 
employment 
benefits 

Share-based 
payment 

Total 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

36 

28 

76 

31 

84 

33 

288 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

87 

160 

-- 

99 

-- 

92 

438 

123 

188 

76 

130 

84 

125 

726 

Mr. Dinko Valerio   

Mr. Henri Termeer 

Mr. Antoine Papiernik 

Ms. Alison Lawton  

Mr. Paul Baart 

Mr. James Shannon 

As at December 31, 2018:  

  Mr. Dinko Valerio holds 1,043,420 ordinary shares in the Company, as well as 115,925 options. These 

options vest in four annual equal tranches of 25% starting for the first time as of the first anniversary of 

the date of grant. In 2017, Mr. Valerio was granted 32,164 options under the Option Plan to acquire 

depositary receipts issued for ordinary shares at an average exercise price of € 4.65 per option. In 2018, 

Mr. Valerio was granted 27,500 options at an average exercise price of € 2.74 per option. 

On September 12, 2017, Mr. Valerio provided a convertible loan to Amylon Therapeutics B.V. This loan is 

interest-bearing at an average rate of 8% per annum and is convertible into a variable number of 

ordinary shares within 36 months at the option of the holder or the Company in case financing criteria 

are met. Any unconverted loans become payable on demand after 24 months in equal quarterly terms. 

  Mr. Antoine Papiernik does not hold any shares or options in the Company. As a managing partner of 

Sofinnova Partners SAS, the management company of Sofinnova Capital VII FCPR, holder of 2,764,194 

ordinary shares, Mr. Papiernik may be deemed to have share voting and investment power with respect 

to such shares. 

  Ms. Alison Lawton holds 96,473 options. In 2017, Ms. Lawton was granted 32,164 options under the 

Option Plan to acquire depositary receipts issued for ordinary shares with an average exercise price of 

€ 4.65 per option. In 2018, Ms. Lawton was granted 27,500 options with an average exercise price of 

€ 2.74 per option. Under these option grants options vest in four annual equal tranches of 25% starting 

for the first time as of the first anniversary of the date of grant. 

  Mr. Paul Baart does not hold any shares or options in the Company. 
  Mr. James Shannon holds 61,538 ordinary shares in the Company and 92,733 options. In 2017, Mr. 

Shannon was granted 32,164 options under the Option Plan to acquire depositary receipts issued for 

ordinary shares at an exercise price of € 4.65 per option. In 2018, Mr. Shannon was granted 27,500 

options at an exercise price of € 2.74 per option. Under these option grants options vest in four annual 

equal tranches of 25% starting for the first time as of the first anniversary of the date of grant. 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Our Management Board is supported by our officers, or senior management. The total remuneration of the 

Management Board and senior management in 2018 amounted to € 5,481,000. 

The details are set out in the table below:  

dsssds  

2018 

Mr. D.A. de Boer1 

Mr. R.K. Beukema2 

Management Board 

Senior Management 

Short term 
employee 
benefits 

Post 
employment 
benefits 

Share-based 
payment 

Total 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

726 

809 

1,535 

1,726 

3,261 

9 

16 

25 

64 

89 

668 

464 

1,132 

999 

2,131 

1,403 

1,289 

2,692 

2,789 

5,481 

1  
2  

Short term employee benefits includes a bonus for Mr. Daniel de Boer of € 281,000 based on goals realised in 2018. 
Short term employee benefits includes a bonus for Mr. René Beukema of € 134,000 based on goals realised in 2018 
and a severance payment of € 324,000 

The total remuneration of the Management Board and senior management in 2017 amounted to € 5,096,000 

with the details set out in the table below:  

dsssds  

2017 

Mr. D.A. de Boer1 

Mr. R.K. Beukema2 

Management Board 

Senior Management 

Short term 
employee 
benefits 

Post 
employment 
benefits 

Share-based 
payment 

Total 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

570 

411 

981 

1,719 

2,700 

8 

15 

23 

66 

89 

622 

261 

883 

1,424 

2,307 

1,200 

687 

1,887 

3,209 

5,096 

1  
2  

Short term employee benefits includes a bonus for Mr. Daniel de Boer of € 217,000 based on goals realised in 2017. 
Short term employee benefits includes a bonus for Mr. René Beukema of € 113,000 based on goals realised in 2017. 

As at December 31, 2018:  

  Mr. Daniel de Boer holds 705,309 ordinary shares in the Company as well as 828,623 options. In 2017, 

Mr. de Boer was awarded 239,717 options to acquire ordinary shares at an exercise price of € 4.65 per 

option. In 2018, he was awarded 379,285 options at an exercise price of € 2.74 per option. These options 

vest over four years in equal annual installments and had a remaining weighted-average contractual life 

of 8.1 years at December 31, 2018.  

  Mr. René Beukema holds 346,239 ordinary shares in the Company as well as 440,013 options. In 2017, 

Mr. Beukema was awarded 101,408 options to acquire ordinary shares at an exercise price of € 4.65 per 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
option. In 2018, he was awarded 140,932 options at an exercise price of € 2.74 per option. These options 

vest over four years in equal annual installments and had a remaining weighted-average contractual life 

of 7.2 years at December 31, 2018. Mr. Beukema left the Company January 1, 2019. 

ProQR does not grant any loans, advanced payments and guarantees to members of the Management and 

Supervisory Board. 

On March 26, 2019, the Company announced the strategic spin out of the Dystrophic Epidermolysis Bullosa 

(DEB) activities into the newly formed company, Wings Therapeutics. This company is formed and financed by 

EB Research Partnership (EBRP), the largest global non-profit dedicating to treating and curing EB. Wings 

Therapeutics will focus on developing therapies for DEB and continue to conduct clinical trials with QR-313 in 

exon 73 as well as progress other RNA molecules that are designed for other mutations that cause DEB. 

ProQR has a minority stake in Wings Therapeutics and will be eligible for milestone and royalty rights to 

commercial products. The financial impact on the Company is estimated to be immaterial. 

 
 
 
 
 
 
 
 
 
(Before appropriation of result) 

dsssds  

ASSETS 

Non-current assets 

Financial fixed assets 

Current assets 

Social securities and other taxes 

Prepayments and other receivables 

Cash and cash equivalents 

TOTAL ASSETS 

EQUITY 

Shareholders' equity 

Share capital 

Share premium reserve 

Equity settled employee benefits reserve 

Translation reserve 

Accumulated deficit 

Unappropriated result 

LIABILITIES 

Provisions 

Non-current liabilities 

Borrowings 

Current liabilities 

Trade payables 

Social securities and other taxes 

Pension premiums 

Deferred income 

Other current liabilities 

Note 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

27 

28 

29 

30 

31 

32 

33 

70 

70 

291 

31,885 

100,560 

132,736 

0 

0 

379 

20,615 

47,029 

68,023 

132,806 

68,023 

1,726 

235,744 

10,780 

108 

(118,396) 

(36,126) 

93,836 

1,457 

148,763 

8,377 

136 

(75,733) 

(43,484) 

39,516 

30,214 

20,710 

7,351 

7,351 

79 

-- 

6 

-- 

1,320 

1,405 

6,582 

6,582 

184 

214 

-- 

347 

470 

1,215 

TOTAL LIABILITIES 

38,970 

28,507 

TOTAL EQUITY AND LIABILITIES 

132,806 

68,023 

The accompanying notes are an integral part of these financial statements. 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
dsssds  

Note 

Share in results of participating interests, after taxation 

27 

Other result after taxation 

Net result for the year 

The accompanying notes are an integral part of these financial statements. 

2018 

€ 1,000 

(31,932) 

(4,194) 

2017 

€ 1,000 

(34,123) 

(9,361) 

(36,126) 

(43,484) 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
The company financial statements are part of the 2018 financial statements of ProQR Therapeutics N.V. (the 

‘Company’) and have been prepared in accordance with the legal requirements of Part 9, Book 2 of the 

Netherlands Civil Code. 

With reference to the income statement of the company, use has been made of the exemption pursuant to 

Section 402 of Book 2 of the Netherlands Civil Code. 

For setting the principles for the recognition and measurement of assets and liabilities and determination of 

the result for its company financial statements, the Company makes use of the option provided in section 

2:362(8) of the Netherlands Civil Code. This means that the principles for the recognition and measurement 

of assets and liabilities and determination of the result (hereinafter referred to as principles for recognition 

and measurement) of the company financial statements of the Company are the same as those applied for 

the consolidated IFRS financial statements. See page 55 for a description of these principles. 

Participating interests in group companies are valued using the equity method, applying the IFRS accounting 

policies endorsed by the European Union. Following the adoption of IFRS 9 by the group, and our 

interpretation of the Dutch Accounting Standard 100.107A, the company shall, upon identification of a credit 

loss on an intercompany loan and/or receivable, eliminate the carrying amount of the intercompany loan 

and/or receivable for the value of the identified credit loss. 

The share in the result of participating interests consists of the share of the Company in the result of these 

participating interests. In so far as gains or losses on transactions involving the transfer of assets and 

liabilities between the Company and its participating interests or between participating interests themselves 

can be considered unrealised, they have not been recognised. 

dsssds  

Participating interests in group companies 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

70 

70 

0 

0 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Movements in financial fixed assets were as follows: 

dsssds  

Net asset value as of January 1 

Share in results of participating interests, after taxation 

Exchange differences 

Change in provisions for negative net asset value 

Net asset value as of December 31 

Participating 
interests  
in group 
 companies  

Total 

€ 1,000 

€ 1,000 

0 

(31,932) 

(28) 

32,030 

70 

0 

(31,932) 

(28) 

32,030 

70 

At December 31, 2018, the Company, having its statutory seat in Leiden, the Netherlands, is the ultimate 

parent company of the following consolidated participating interests: 

Name 

ProQR Therapeutics Holding B.V. 

ProQR Therapeutics I B.V. 

ProQR Therapeutics II B.V. 

ProQR Therapeutics III B.V. 

ProQR Therapeutics IV B.V. 

ProQR Therapeutics VI B.V. 

ProQR Therapeutics VII B.V. 

ProQR Therapeutics VIII B.V. 

ProQR Therapeutics IX B.V. 

ProQR Therapeutics I Inc. 

Amylon Therapeutics B.V. 

Amylon Therapeutics Inc. 

Location 

Share in issued capital 

Leiden, the Netherlands 

Leiden, the Netherlands 

Leiden, the Netherlands 

Leiden, the Netherlands 

Leiden, the Netherlands 

Leiden, the Netherlands 

Leiden, the Netherlands 

Leiden, the Netherlands 

Leiden, the Netherlands 

Delaware, United States 

Leiden, the Netherlands 

Delaware, Unites States 

100% 

100% 

100% 

100% 

100% 

100% 

100% 

100% 

100% 

100% 

80% 

80% (100% held by Amylon 
Therapeutics B.V. 

ProQR Therapeutics Holding B.V. is an intermediate holding company and the only subsidiary owned directly 

by ProQR Therapeutics N.V..  

ProQR Therapeutics N.V. is also statutory director of Stichting Bewaarneming Aandelen ProQR (“ESOP 

Foundation”). For details on the accounts receivable from participating interests and the other receivables, 

reference is made to note 29. 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
dsssds  

Value added tax 

All receivables are considered short-term and due within one year.  

dsssds  

Accounts receivable from group companies 

Prepayments 

Other receivables 

All receivables are considered short-term and due within one year.  

dsssds  

Cash at banks 

Bank deposits 

The cash at banks is at full disposal of the Company.  

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

291 

291 

379 

379 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

31,719 

20,400 

166 

-- 

210 

5 

31,885 

20,615 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

100,560 

-- 

100,560 

47,029 

-- 

47,029 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Balance at January 1, 
2017 

Retained result 

Foreign exchange 
differences 

Recognition of share-
based payments 

Issue of ordinary shares 

Issue of treasury shares 

Share options exercised 

Result for the year 

Balance at 
December 31, 2017 

Retained result 

Foreign exchange 
differences 

Recognition of share-
based payments 

Share 
Capital 

Share 
Premium 

Equity 
Settled 
Employee 
Benefit 
Reserve 

Trans-
lation 
Reserve 

Accumu-
lated 
Deficit 

Unappro-
priated 
result 

Total 
Equity 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

€ 1,000 

934 

123,597 

4,353 

(15) 

(36,630) 

(39,103) 

53,136 

-- 

(39,103) 

39,103 

-- 

-- 

-- 

343 

180 

0 

-- 

-- 

-- 

-- 

25,342 

(180) 

4 

-- 

-- 

-- 

4,024 

-- 

-- 

-- 

-- 

151 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

(43,484) 

(43,484) 

-- 

151 

4,024 

25,685 

-- 

4 

-- 

(28) 

5,413 

84,191 

-- 

870 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

1,457 

148,763 

8,377 

136 

(75,733) 

(43,484) 

39,516 

-- 

-- 

4 

-- 

-- 

-- 

-- 

2,185 

3,224 

Issue of ordinary shares 

265 

83,926 

Share options lapsed 

Share options exercised 

Result for the year 

-- 

-- 

-- 

-- 

870 

-- 

(97) 

(724) 

-- 

-- 

(43,484) 

43,484 

(28) 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

-- 

97 

724 

-- 

(36,126) 

(36,126) 

Balance at 
December 31, 2018 

1,726 

235,744 

10,780 

108 

(118,396) 

(36,126) 

93,836 

The 2017 result was added to the accumulated deficit in accordance with the resolution of the Annual 

General Meeting of shareholders. At the upcoming Annual General Meeting of shareholders, it will be 

proposed to add the 2018 result to the accumulated deficit. For more details we refer to note 12 to the 

consolidated financial statements. 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
dsssds  

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

Shareholders’ equity according to the consolidated balance sheet 

Share in results of participating interests with negative equity 

Shareholders’ equity according to the company balance sheet 

92,685 

1,151 

93,836 

39,325 

191 

39,516 

dsssds  

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

Net result according to the consolidated profit and loss account 

(37,086) 

(43,675) 

Share in results of participating interests with negative equity 

Net result according to the company profit and loss account 

960 

191 

(36,126) 

(43,484) 

dsssds  

December 31, 
2018 

December 31, 
2017 

Provision for negative equity group companies 

€ 1,000 

€ 1,000 

Balance at January 1 

Provisions made during the year 

Balance at December 31 

20,710 

9,504 

30,214 

12,175 

8,535 

20,710 

dsssds  

Innovation credit 

Accrued interest 

Total borrowings 

December 31, 
2018 

December 31, 
2017 

€ 1,000 

€ 1,000 

5,000 

2,351 

7,351 

4,899 

1,683 

6,582 

Innovation credit (“Innovatiekrediet”)  

On June 1, 2012, ProQR was awarded an Innovation credit by the Dutch government, through its agency RVO 

of the Ministry of Economic Affairs, for the Company’s cystic fibrosis program. Amounts were drawn under 

this facility in the course of the years 2013 through 2017. The credit covers 35% of the costs incurred in 

respect of the program up to € 5.0 million.  

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
The credit is interest-bearing at a rate of 10% per annum. Early October 2018 ProQR received a conditional 

waiver of the € 5 million Innovation credit. The total loan of € 7.4 million including interest will be waived after 

3 years if certain conditions are met. The conditions are reviewed by RVO on an annual basis. 

The assets which are co-financed with the granted innovation credits are subject to a right of pledge for the 

benefit of RVO. 

There are no claims known to management related to the activities of the Company.  

In August 2014, the Company entered into an agreement with Cystic Fibrosis Foundation Therapeutics, Inc., 

or CFFT, a subsidiary of the Cystic Fibrosis Foundation, pursuant to which CFFT agreed to provide the 

Company with up to $ 3 million (€ 2.6 million) to support the clinical development of eluforsen. 

Pursuant to the terms of the agreement, the Company is obligated to make a one-time milestone payment to 

CFFT of up to approximately $ 16 million (€ 14 million), payable in three equal annual installments following 

the first commercial sale of eluforsen, the first of which is due within 90 days following the first commercial 

sale. The Company is also obligated to make a one-time milestone payment to CFFT of up to $ 3 million (€ 2.6 

million) if net sales of eluforsen exceed $ 500 million (€ 437 million) in a calendar year. Lastly, the Company is 

obligated to make a payment to CFFT of up to approximately $ 6 million (€ 5 million) if it transfers, sells or 

licenses eluforsen other than for certain clinical or development purposes, or if the Company enters into a 

change of control transaction prior to commercialization. However, the payment in the previous sentence 

may be set-off against the $ 16 million milestone payment. Either CFFT or the Company may terminate the 

agreement for cause, which includes the Company’s material failure to achieve certain commercialization and 

development milestones. The Company’s payment obligations survive the termination of the agreement. 

The Company has issued declarations of joint and several liabilities for debts arising from the actions of 

Dutch consolidated participating interests, as meant in article 2:403 of the Netherlands Civil Code. 

The Company constitutes a tax entity with its Dutch subsidiaries for corporate income tax purposes; the 

standard conditions prescribe that all companies of the tax entity are jointly and severally liable for the 

corporate income tax payable. 

 
 
 
 
 
 
The fees for services provided by our external auditor, Deloitte Accountants B.V., are specified below for each 

of the financial years indicated: 

dsssds  

Audit fees 

Audit-related fees 

Tax fees 

All other fees 

Audit fees 

2018 

€ 1,000 

2017 

€ 1,000 

181 

261 

-- 

-- 

442 

175 

140 

-- 

-- 

315 

Consist of aggregate fees for professional services provided in connection with the annual audit of our 

financial statements, procedures on our quarterly financial statements, consultations on accounting matters 

directly related to the audit, and comfort letters, consents and assistance with and review of documents filed 

with the SEC.  

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Leiden, March 28, 2019, 

D.A. de Boer 

D. Valerio 

A.B. Papiernik 

A. Lawton 

P.R. Baart 

J.S.S. Shannon 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Independent auditor’s report  

Reference is made to the independent auditor’s report as included hereinafter. 

Statutory arrangement concerning the appropriation of the result 

In Article 21 of the Company statutory regulations the following has been presented concerning the 

appropriation of result: 

1. 

2. 

The profit is at the free disposal of the General Meeting of Shareholders. 

The Company may only distribute profits to shareholders and other recipients to distributable profits to 

the extent that the equity exceeds the paid-up capital plus the reserves required by law. 

3.  Distribution of profits shall take place after adoption of the annual accounts from which it becomes 

clear that distribution is permissible. 

4.  When calculating the distribution of profits shares held by the Company shall be disregarded, unless 

this shares has been encumbered with usufruct or right of pledge or certificates thereof are issued as a 

result of which the entitlement to profits accrue to the usufructuary, pledgee or holder of the 

certificates. 

5. 

Certificates held by the Company or whereon the Company holds limited rights as a result of which the 

Company is entitled to distribution of profits shall also be disregarded when calculating the distribution 

of profits. 

6. 

The Company may make interim distributions, only if the requirements in paragraph 2 are met.  

 
 
 
 
 
To the Shareholders and the Supervisory Board of ProQR Therapeutics N.V.  

Our opinion 

We have audited the accompanying financial statements 2018 of ProQR Therapeutics N.V., based in Leiden, 

The Netherlands. The financial statements include the consolidated financial statements and the company 

financial statements. 

In our opinion: 

 

The accompanying consolidated financial statements give a true and fair view of the financial position of 

ProQR Therapeutics N.V. as at December 31, 2018, and of its result and its cash flows for 2018 in 

accordance with International Financial Reporting Standards as adopted by the European Union (EU-IFRS) 

and with Part 9 of Book 2 of the Dutch Civil Code. 

 

The accompanying company financial statements give a true and fair view of the financial position of 

ProQR Therapeutics N.V. as at December 31, 2018, and of its result for 2018 in accordance with Part 9 of 

Book 2 of the Dutch Civil Code. 

The consolidated financial statements comprise: 

1.  The consolidated statement of financial position as at December 31, 2018. 
2.  The following statements for 2018: the consolidated statement of profit or loss and comprehensive 

income, consolidated statement of changes in equity and consolidated statement of cash flows. 

3.  The notes comprising a summary of the significant accounting policies and other explanatory 

information.  

The company financial statements comprise: 

1.  The company balance sheet as at December 31, 2018. 

2.  The company income statement for the year ended December 31, 2018. 
3.  The notes comprising a summary of the accounting policies and other explanatory information. 

Basis for our opinion 

We conducted our audit in accordance with Dutch law, including the Dutch Standards on Auditing. Our 

responsibilities under those standards are further described in the "Our responsibilities for the audit of the 

financial statements" section of our report. 

We are independent of ProQR Therapeutics N.V. in accordance with the EU Regulation on specific 

requirements regarding statutory audit of public-interest entities, the Wet toezicht accountantsorganisaties 

(Wta, Audit firms supervision act), the Verordening inzake de onafhankelijkheid van accountants bij 

assurance-opdrachten (ViO, Code of Ethics for Professional Accountants, a regulation with respect to 

independence) and other relevant independence regulations in the Netherlands. Furthermore, we have 

complied with the Verordening gedrags- en beroepsregels accountants (VGBA, Dutch Code of Ethics). 

We believe the audit evidence we have obtained is sufficient and appropriate to provide a basis for our 

opinion. 

 
 
 
 
 
Materiality 

Based on our professional judgement we determined the materiality for the consolidated financial statements 

as a  whole at € 2.000.000. The materiality is based on 5% of total operating costs which is consistent with 

prior year. We have also taken into account misstatements and/or possible  misstatements that in our opinion 

are material for the users of the financial statements for qualitative reasons. 

We agreed with the Supervisory Board that misstatements in excess of € 95.000, which are identified  during 

the audit, would be reported to them, as well as smaller misstatements that in our view must be  reported on 

qualitative grounds.  

Scope of the group audit 

ProQR Therapeutics N.V. is at the head of a group of entities. The financial information of this group is 

included in the consolidated financial statements of ProQR Therapeutics N.V. 

The financial administration for all group entities is centralized in the Netherlands. Consequently, we have 

centralized our audit approach and we have been able to obtain sufficient and appropriate audit evidence 

about the group’s financial information to provide an opinion about the financial statements.  

Our key audit matters 

Key audit matters are those matters that, in our professional judgement, were of most significance in our 

audit of the financial statements. We have communicated the key audit matters to the Supervisory Board. 

The key audit matters are not a comprehensive reflection of all matters discussed. 

These matters were addressed in the context of our audit of the financial statements as a whole and in 

forming our opinion thereon, and we do not provide a separate opinion on these matters. Our key audit 

matters of 2018 are consistent with those identified during prior year’s audit.  

Research and development expenses 

Description 

How the key audit matter was addressed in  

The total research and development expenses for 

the audit 

the year 2018 amount to € 29.5 million. These 

We tested the key control for design and 

research and development expenses consist of 

implementation. We decided not to rely on controls 

payroll costs of employees as well as outsourced 

and tested the R&D expenses substantively. Our 

research and development activities with third party 

audit procedures included, amongst others, the 

suppliers. The research and development activities 

review of the agreements with suppliers and the 

with these suppliers are concluded in master service 

related accounting evaluation as well as the timing 

agreements and statements of work. These 

of expenses recognized. In addition, we tested the 

outsourced research and development activities are 

progress of projects based on confirmations sent to 

typically performed over a period of time and as a 

significant vendors, we performed inquiries of 

consequence the allocation of expenses to the 

project managers and inspected purchase orders 

reporting period is based on the progress of the 

and work orders in order to determine the correct 

work which involves (significant) judgement.  

cut-off of R&D expenses and accruals. 

The R&D expenses are disclosed in note 16 of the 

financial statements.  

Observation 

The scope and nature of the procedures performed 

were sufficient and appropriate to address the risks 

of material misstatement in R&D expenses. 

 
 
 
 
 
 
Significant contracts 

Description 

How the key audit matter was addressed in  

ProQR Therapeutics N.V. concluded several 

the audit 

significant contracts, such as the above mentioned 

We tested the key controls for design and 

research and development agreements. These 

implementation. We decided not to rely on controls 

contracts contain terms and conditions that may 

and tested the significant contracts substantively. 

require complex accounting and/or significant long-

Our audit procedures included, amongst others, the 

term commitments that require disclosure in the 

review of the contract register, obtaining external 

financial statements.  

confirmations on significant R&D contracts and the 

The commitments and contingencies are disclosed 

review of the contract terms and related accounting 

in note 22 of the financial statements.  

evaluation of the impact on the financial statements 

including disclosures of the commitments.  

Observation 

The scope and nature of the procedures performed 

were sufficient and appropriate to address the risk 

of material misstatements of commitments and 

contingencies related to the significant contracts. 

Cash and cash equivalents 

Description 

How the key audit matter was addressed in  

The total cash and cash equivalents as per 

the audit 

December 31, 2018 amount to € 105.6 million. We 

We tested the key controls for design and 

focused on this area as the cash and cash 

implementation. We decided not to rely on control 

equivalents are significant to the financial 

and tested the cash and cash equivalents 

statements. In addition, the availability of sufficient 

substantively. Our procedures included detailed 

cash and cash equivalents is also important as it 

reconciliations of all bank balances to bank 

provides information of the company’s ability to 

confirmations, recalculating foreign exchange 

continue as a going concern and fund its planned 

results on these balances and a review of the 

R&D activities.  

statements, confirmations and underlying 

Cash and cash equivalents are disclosed in note 11 

agreements for deposit balances to assess the 

of the financial statements. 

presentation and disclosure in the financial 

statements. 

Observation 

The scope and nature of the procedures performed 

were sufficient and appropriate to address the risks 

of material misstatement in the cash and cash 

equivalents. 

REPORT ON THE OTHER INFORMATION INCLUDED IN THE ANNUAL ACCOUNTS   

In addition to the financial statements and our auditor's report thereon, the annual accounts  contain other 

information that consists of: 

  Management Board’s Report. 
  Other Information as required by Part 9 Book 2 of the Dutch Civil Code. 

 
 
 
 
  
 
 
 
 
Based on the following procedures performed, we conclude that the other information: 

 

Is consistent with the financial statements and does not contain material misstatements. 

  Contains the information as required by Part 9 of Book 2 of the Dutch Civil Code.  

We have read the other information. Based on our knowledge and understanding obtained through our audit 

of the financial statements or otherwise, we have considered whether the other information contains 

material misstatements. 

By performing these procedures, we comply with the requirements of Part 9 of Book 2 of the Dutch Civil Code 

and the Dutch Standard 720. The scope of the procedures performed is substantially less than the scope of 

those performed in our audit of the financial statements. 

Management is responsible for the preparation of the other information, including the Management Board’s 

Report in accordance with Part 9 Book 2 of the Dutch Civil Code, and the other information as required by 

Part 9 Book 2 of the Dutch Civil Code. 

REPORT ON OTHER LEGAL AND REGULATORY REQUIREMENTS 

Engagement 

We were engaged by the Supervisory Board as auditor of ProQR Therapeutics N.V. starting with the audit for 

the year 2012 and have operated as statutory auditor ever since that financial year. 

No prohibited non-audit services 

We have not provided prohibited non-audit services as referred to in Article 5(1) of the EU Regulation on 

specific requirements regarding statutory audit of public-interest entities. 

DESCRIPTION OF RESPONSIBILITIES REGARDING THE FINANCIAL STATEMENTS 

Responsibilities of management and Supervisory Board  for the financial statements 

Management is responsible for the preparation and fair presentation of the financial statements in 

accordance with EU-IFRS and Part 9 of Book 2 of the Dutch Civil Code. Furthermore, management is 

responsible for such internal control as management determines is necessary to enable the preparation of 

the financial statements that are free from material misstatement, whether due to fraud or error. 

As part of the preparation of the financial statements, management is responsible for assessing the 

company's ability to continue as a going concern. Based on the financial reporting frameworks mentioned, 

management should prepare the financial statements using the going concern basis of accounting unless 

management either intends to liquidate the company or to cease operations, or has no realistic alternative 

but to do so.  

Management should disclose events and circumstances that may cast significant doubt on the company's 

ability to continue as a going concern in the financial statements. 

The supervisory board is responsible for overseeing the company's financial reporting process. 

Our responsibilities for the audit of the financial statements 

Our objective is to plan and perform the audit assignment in a manner that allows us to obtain sufficient and 

appropriate audit evidence for our opinion. 

 
 
 
Our audit has been performed with a high, but not absolute, level of assurance, which means we may not 

detect all material errors and fraud during our audit. 

Misstatements can arise from fraud or error and are considered material if, individually or in the  aggregate, 

they could reasonably be expected to influence the economic decisions of users taken on  the basis of these 

financial statements. The materiality affects the nature, timing and extent of our  audit procedures and the 

evaluation of the effect of identified misstatements on our opinion. 

We have exercised professional judgement and have maintained professional skepticism throughout the 

audit, in accordance with Dutch Standards on Auditing, ethical requirements and independence 

requirements. Our audit included e.g.: 

 

Identifying and assessing the risks of material misstatement of the financial statements, whether due to 

fraud or error, designing and performing audit procedures responsive to those risks, and obtaining audit 

evidence that is sufficient and appropriate to provide a basis for our opinion. The risk of not detecting a 

material misstatement resulting from fraud is higher than for one resulting from error, as fraud may 

involve collusion, forgery, intentional omissions, misrepresentations, or the override of internal control. 
  Obtaining an understanding of internal control relevant to the audit in order to design audit procedures 

that are appropriate in the circumstances, but not for the purpose of expressing an opinion on the 

effectiveness of the company's internal control. 

 

Evaluating the appropriateness of accounting policies used and the reasonableness of accounting 

estimates and related disclosures made by management. 

  Concluding on the appropriateness of management's use of the going concern basis of accounting, and 

based on the audit evidence obtained, whether a material uncertainty exists related to events or 

conditions that may cast significant doubt on the company's ability to continue as a going concern. If we 

conclude that a material uncertainty exists, we are required to draw attention in our auditor's report to 

the related disclosures in the financial statements or, if such disclosures are inadequate, to modify our 

opinion. Our conclusions are based on the audit evidence obtained up to the date of our auditor's report. 

However, future events or conditions may cause the company to cease to continue as a going concern. 

Evaluating the overall presentation, structure and content of the financial statements, including the 

disclosures.  

Evaluating whether the financial statements represent the underlying transactions and events in a 

manner that achieves fair presentation. 

 

 

Because we are ultimately responsible for the opinion, we are also responsible for directing, supervising and 

performing the group audit. In this respect we have determined the nature and extent of the audit 

procedures to be carried out for group entities. Decisive were the size and/or the risk profile of the group 

entities or operations. On this basis, we selected group entities for which an audit or review had to be carried 

out on the complete set of financial information or specific items. 

We communicate with the supervisory board regarding, among other matters, the planned scope and timing 

of the audit and significant audit findings, including any significant findings in internal control that we 

identified during our audit. In this respect we also submit an additional report to the audit committee in 

accordance with Article 11 of the EU Regulation on specific requirements regarding statutory audit of public-

interest entities. The information included in this additional report is consistent with our audit opinion in this 

auditor's report. 

We provide the Supervisory Board with a statement that we have complied with relevant ethical requirements 

regarding independence, and to communicate with them all relationships and other matters that may 

reasonably be thought to bear on our independence, and where applicable, related safeguards. 

 
 
 
 
From the matters communicated with the Supervisory Board, we determine the key audit matters: those 

matters that were of most significance in the audit of the financial statements. We describe these matters in 

our auditor's report unless law or regulation precludes public disclosure about the matter or when, in 

extremely rare circumstances, not communicating the matter is in the public interest.  

Amsterdam, March 28, 2019 

I.A. Buitendijk 

 
 
 
 
Want to learn more?

Read on about ProQR  
and our programs
proqr.com

Check out our video “How do 
ProQR’s RNA therapies work?”
proqr.com/rna-video

Follow the latest  
developments in our pipeline
proqr.com/pipeline

View our complete  
Annual Report online
proqr.com/ar2018

Download the 
Thru My Eyes app 
thrumyeyes.app

ProQR Therapeutics N.V.
T  : +31 88 166 7000 
W : www.proqr.com 
E  : info@proqr.com

Headquarters Leiden
Zernikedreef 9
2333 CK Leiden,
the Netherlands

Office Cambridge
245 Main Street (2nd Floor)
Cambridge, MA 02142
USA