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Assembly Biosciences, Inc.

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FY2015 Annual Report · Assembly Biosciences, Inc.
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ASSEMBLY BIOSCIENCES 
2015 ANNUAL SHAREHOLDERS REPORT 

FIRST YEAR OF POST-MERGER OPERATIONS: 
BUILDING A FOUNDATION FOR FUTURE SUCCESS 

DEAR SHAREHOLDERS: 

company  discovering,  developing 
and  commercializing  novel  HBV 
and microbiome therapies  

this 

January 

ESTABLISHING THE 
FOUNDATION WITH A WORLD 
CLASS TEAM 
year  we 
In 
announced  several  new  additions 
to  our  senior  management  team. 
The  team  now  includes  deeply 
experienced  drug-hunters  and 
have 
developers  who 
drug 
collectively 
and 
discovered 
brought  to  market  more  than  10 
commercially  successful  products 
in  infectious  disease  (ID),  gastro-
enterology 
(GI)  disorders  and 
various other disease indications. 

50 

We  have  built  out  our  research 
and  development  teams  in  both 
our  HBV 
and  Microbiome 
programs  and  now  number 
approximately 
full-time 
employees  and  roughly  35  FTE 
chemistry  and  other  specialist 
outsourced  team  members.  The 
majority  of  our  R&D  is  conducted 
in  our  research  facility  in  San 
Francisco  and  supplemented  by 
focused  teams  in  Indiana  and 
New York. We are now leveraging 
these 
to  advance  
our  discovery  and  development 
programs 
clinical 
towards 
development & commercialization.   

resources 

Derek A. Small, 
President and 
Chief Executive Officer 

Just  over  a  year  and  a  half  ago 
we  formed  Assembly  Biosciences 
through  a  merger  of  a  public  and 
private  company 
focus  on 
highly  innovative  programs  aimed 
at achieving curative therapies.  

to 

We started with a small combined 
team  of  about  10  people  and  two 
separate  programs  addressing 
B 
hepatitis 
(HBV) 
infection 
and  microbiome 
therapeutics. 

virus 

significantly 

Today  we  have  advanced  these 
programs 
and 
established  what  we  believe  to  be 
a  world  class  team  and  focused 
company  committed  to  achieving 
our  ambitious  goals  of  becoming 
biotechnology 
an 

integrated 

HBV-CURE PROGRAM – 
MOVING OUR FIRST DRUG 
CANDIDATE INTO THE CLINIC 
More  effective  cures  for  HBV  are 
clearly  needed.  Currently  there 
are  over  240  million  people 
infected 
chronically 
worldwide 
with  HBV;  with  over  90  million  in 
China.  Unlike  hepatitis  C  virus 
infection  (HCV),  where  cure  rates 
are now nearing 99%, the current 
cure rates for HBV are less than 
5%,  and  chronic  HBV  infection  is 
associated  with  high  rates  of 
disability  and  early  death  from 
cancer  and  other  liver  diseases  – 
globally  over  500,000  deaths 
estimated annually.  

This  is  not  acceptable,  and  we 
believe  our  scientific  and  drug 
discovery  insights  represent  an 
opportunity 
this 
deficiency.  

address 

to 

program 

Our  HBV 
began 
modestly  in  the  lab  of  Dr.  Adam 
Indiana  University 
Zlotnick  at 
that 
vision 
his 
on 
based 
modulating  the  multiple  functions 
of  the  HBV  core  protein  (HBc) 
might represent a new therapeutic 
modality  with  curative  potential. 
We  have  now  built  our  chemistry, 
biochemistry,  and  biology  teams 

2015 KEY ACHIEVEMENTS 

 Completed +$80 million capital raise providing funding to key inflection points 
 Recruited R&D teams with record of success & world-class expertise 
 Presented  human  clinical  data  demonstrating  Gemicel™  can  target  bolus  delivery  to 

lower GI tract 

 Identified new CpAM class targeting HBV core protein with multiple potential leads 

 
                                                                                                                                                                                               
 
 
 
 
 
 
 
 
  
 
 
 
  
future  and  established  a  research 
to  generate  multiple 
engine 
molecules 
that  are  potential 
candidates  for  the  combination 
regimens  we  believe  will  likely  be 
required 
to  cure  HBV.  We 
anticipate  entering  Phase  1 
clinical  trials  with  our  first  HBV 
candidate  during  the  second  half 
of 2016. 

MICROBIOME PROGRAM –  
A FULLY-INTEGRATED 
PLATFORM FOR 
DISCOVERING, cGMP 
MANUFACTURING AND 
ORALLY DELIVERING 
TARGETED MICROBIOTIC 
DRUGS 

We  believe  that  we  have  the 
potential  to  become  one  of  the 
leading  microbiome  companies  in 
the world.  

We  understand  this  is  a  bold 
statement, but there are a number 
of  reasons  we  believe  this  to  
be true.  

The  Assembly  team  has  been 
working  on  our  microbiome 
programs  for  over  four  years  - 
focusing  diligently  on  research 
and  development  activities 
to 
increase  our  confidence  in  the 
program’s three main elements: 

to  a  level  of  sophistication  and 
novel  R&D  expertise 
that  we 
believe rivals any in the HBV field. 
Our  team  has  established  distinct 
chemical series for the modulation 
of  HBc  and  other  targets  relevant 
to  achieving  cures 
this 
devastating  disease,  along  with 
innovative assays and biomarkers 
to facilitate the R&D process. 

for 

In  December  we  announced  the 
selection  of  our  first  lead  HBV 
candidate,  which  is  now  in  IND-
enabling development. In addition, 
we  have  advanced  a  number  of 
other  molecules  toward  possible 
candidate  selection  in  the  near 

Bacterial Strain Selection 

from 

We  are  building  a  fully  synthetic 
microbiome  product 
the 
ground  up.  To  succeed,  we  first 
set  out  to  select  human  bacteria 
strains 
that  would  have  both 
therapeutic properties and also be 
amenable  to  being  produced  as 
have 
biologic 
successfully 
our 
program  for  microbiome  bacterial 
strain  discovery  and  selection, 
both internally and in collaboration 
leading 
of 
with 
the 
academic 
and 
scientists 
microbiome  research  companies 
in the field. 

drugs.  We 

established 

some 

to 

Delivery of Microbiota 
Selectively to the GI Tract   
Early on, we realized the field was 
competitive with many entrants.  A 
key  opportunity  was  the  hurdle  of 
specifically 
and 
effectively 
delivering  bacteria 
relevant 
regions of the gastrointestinal (GI) 
tract.  To  address  this,  in  2013  we 
obtained an exclusive license to a 
novel  delivery  technology  called 
Gemicel™  that  allows  for  bolus 
delivery  of  therapeutic  bacteria  to 
the  colon. 
targeted  regions  of 
Earlier  in  2015  we  achieved  a 
major  human  proof  of  concept 
milestone  with  Gemicel;  human 
data  confirming  that  Gemicel  can  

Preparing to advance our lead Microbiome &             

HBV programs into clinical trials in 2016 

Capital efficient and funded through inflection 
points. Proprietary platforms offer  
multiple partnership opportunities 

                                                     2016 PROJECTED MILESTONES 

o Complete senior management expansion √ 

o Initiate ABI-M101 Phase Ib clinical study in 

o Present 1st human study data for GemicelTM 

recurrent CDI patients 

oral colonic delivery technology √ 

o Initiate IND-directed studies on 2nd Microbiome 

o Advance 1st Microbiome program for CDI into 

program candidate 

IND-directed studies √ 

o Present data on HBV-Cure leads at AASLD 2016 

o Present data at 2016 EASL Congress on HBV-

Cure clinical candidates √ 

o Submit IND for 1st Microbiome program -  ABI–

M101 for CDI  

scientific meeting  

o Initiate Phase I clinical trial with 1st HBV-Cure 

molecule in healthy volunteers 

o Select 2nd HBV-Cure program clinical 

candidate  

 
 
 
 
 
                                                                                                                                                                                               
 
 
 
 
 
 
  
 
                                                                                                                                                                                             
 
 
programs  both 
potentially 
partners. 

internally  and 
in  collaboration  with 

COMMITMENT TO PATIENTS 

In  the  midst  of  all  this  activity,  it’s 
important  to  emphasize  that  we 
do  what  we  do  to  help  patients  – 
this  drives  each  one  of  us  in  the 
company.  In  fact,  it’s  core  to  our 
culture, and we intend to keep this 
part  of  our  work  practices  every 
day  as  we  continue  to  develop 
innovative 
and  advance  our 
the  benefit  of 
therapies 
for 
patients.  We 
to 
forward 
look 
reporting on our progress over the 
course  of  this  year,  and  welcome 
your  questions  and  comments, 
which can be sent via the Contact 
section  on  the  Investor  Relations 
page at the Assembly website.  

In closing, I would like to sincerely 
trust  and 
for  your 
thank  you 
support  over  this  past  year.  I’d 
also  like  to  thank  our  employees 
for their commitment to excellence 
and  productivity  in  science  and 
drug  development,  and  certainly 
want 
the 
to 
input  and  strategic 
invaluable 
guidance  of  our  Board  of 
Directors. 

acknowledge 

Sincerely,

Derek A. Small 
President and CEO 
Assembly Biosciences 

the 

successfully  target  bolus  delivery 
to 
ileum  and  colon  were 
presented  at  a  scientific  meeting 
in January 2016. 

cGMP Microbiota 
Manufacturing  

To make microbiotic therapies into 
FDA-regulated  biologic  drugs,  we 
needed  to  be  able  to  reliably  and 
consistently  manufacture 
our 
selected individual bacteria strains 
under  cGMP  conditions  and  to 
scale  the  manufacturing  simply 
and  cost  efficiently.  In  2015,  we 
world-class 
a 
built 
that  can 
manufacturing 
team 
achieve 
cost-
scalable 
effective cGMP manufacture. 

and 

out 

now 

these 

advanced 

We 
key 
components  of  our  Microbiome 
program significantly in 2015, and 
are 
IND-directed 
in 
development  of  our  first  biologic 
drug  candidate,  ABI-M101.  We 
expect  to  initiate  a  Phase  1b 
clinical 
in  patients  with 
recalcitrant  C.  difficile  infections 
(rCDI)  during  the  second  half  of 
2016, and  in parallel advance our 
programs 
into  other  disease 
indications.  

trial 

The potential opportunities for this 
fully integrated microbiotic therapy 
platform  are  many,  and  going 
to  develop 
forward  we  plan 

 
 
 
 
 
 
 
 
 
 
 
 
                                                                                                                                                                                               
 
 
 
 
 
                                                                                                                                                                                            
 
 
MICROBIOME PLATFORM:  DEVELOPING DRUG-LIKE  
MICROBIOME PLATFORM – DEVELOPING DRUG-LIKE ORAL BIOLOGICS 
ORAL BIOLOGICS 

                 PLATFORM FACILITATES THERAPEUTIC AREA & INDICATION EXPANSION 

KEY ADVANTAGES: 
o Microbiota strain selection methods 
o Scalable process development & GMP manufacturing 
o Gemicel™ oral-targeted delivery technology 

• Other 

• Other 

Microbiome Program Pipeline  

 1st Indication:  
C. difficile Infection 
(CDI) 

o  #1 US hospital 

acquired infection; 
CDC urgent threat 

o 29,000 deaths; 
~$5B in costs 

o ABI-M101 clinical 

trial projected in 2H 
2016 

 ABI-M101 
 (Recurrent            
. C. Difficile) 

 ABI-M102 
 (New  
 Indication) 

 ABI-M103 
 (New      
nIndication) 

GemicelTM 
(Oral delivery) 

Strain Library 
& Inventory 

 
 
 
 
 
 
 
 
 
 
 
 
 
HBV-CURE PROGRAM: ADVANCING TOWARDS CLINICAL TRIALS 

R&D ENGINE PRODUCING PROMISING 2ND & 3RD GENERATION CpAMS 
WITH VARIED PROFILES &  ACTIVITY 

 HBV: Millions Affected with Major Unmet Need 

o  >240 million worldwide have chronic HBV 

TO ACHIEVE CLINICAL CURES, 
HBV THERAPY MUST: 

o >90% currently are NOT cured 

o Results in liver disease/death for >500,000 

annually 

Prevent 
New 
cccDNA 

Inhibit 
HBV 
Proteins 

Preclinical Studies Show Clinical Potential 
 of New CpAM Series 

o Potent, pan-genotypic activity across multiple 

viral genotypes 

o Favorable drug characteristics & PK profiles 

o Multiple other series under evaluation – 2nd & 3rd 

generation with varied anti-viral activities 

o Lead candidate advancing towards clinical trials 

in 2H 2016 

CURE 

Eliminate 
(or Silence) Existing 
cccDNA 

HBV-Cure Program Pipeline  

downstream 

 
 
 
 
 
UNITED STATES 
SECURITIES AND EXCHANGE COMMISSION 
WASHINGTON, DC 20549 

FORM 10-K 

 ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 

For the fiscal year ended December 31, 2015 
or 

 TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 

For the Transition Period from __________ to __________ 

Commission File Number: 001-35005 

ASSEMBLY BIOSCIENCES, INC. 
(Exact name of registrant specified in its charter) 

Delaware
(State or Other Jurisdiction of 
Incorporation or Organization) 

2834
(Primary Standard Industrial 
Classification Code Number)

20-8729264
(I.R.S. Employer 
Identification No.)

101 Sixth Avenue, Ninth Floor, New York, New York 10013 
(Address of Principal Executive Offices) 

Registrant’s telephone number, including area code: (646) 706-5208 

Securities Registered Pursuant to Section 12(b) of the Exchange Act: 

Title of Each Class
Common Stock, $0.001 Par Value

Name of Exchange on which Registered
Nasdaq Capital Market

Securities Registered Pursuant to Section 12(g) of the Act: None 

Indicate by check mark if the registrant is a well-known seasoned issuer, as defined in Rule 405 of the Securities Act. Yes No  

Indicate by check mark if the registrant is not required to file reports pursuant to Section 13 or Section 15(d) of the Exchange Act. Yes No  

Indicate by check mark whether the registrant (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange Act of 1934 during the
preceding 12 months (or for such shorter period that the registrant was required to file such reports), and (2) has been subject to such filing requirements for the
past 90 days.  Yes   No  

Indicate by check mark whether the registrant has submitted electronically and posted on its corporate Web site, if any, every Interactive Data File required to be
submitted and posted pursuant to Rule 405 of Regulation S-T during the preceding 12 months (or for such shorter period that the registrant was required to submit
and post such files). Yes  No  

Indicate by check mark if disclosure of delinquent filers pursuant to Item 405 of Regulation S-K is not contained herein, and will not be contained, to the best of 
the registrant’s knowledge, in definitive proxy or information statements incorporated by reference in Part III of this Form 10-K or any amendment to this Form 
10-K.  

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, or a smaller reporting company. See definitions of “large accelerated 
filer,” “accelerated filer” and “smaller reporting company” in Rule 12b-2 of the Exchange Act. (Check one): 
Large accelerated filer 
Non-accelerated filer 

Accelerated filer 
Smaller reporting company 

Indicate by check mark whether the registrant is a shell company (as defined in Rule 12b-2 of the Exchange Act). Yes    No  

The aggregate market value of the voting stock held by non-affiliates of the registrant, as of June 30, 2015, was approximately $236.86 million. Such aggregate
market value was computed by reference to the closing price of the common stock as reported on the Nasdaq Capital Market on June 30, 2015.  For purposes of
making this calculation only, the registrant has defined affiliates as including only (i) directors, (ii) executive officers, and (iii) shareholders that hold greater than
10% of the voting stock of the registrant, in each case, as of June 30, 2015. 

As of March 7, 2016 there were 17,225,662 shares of the registrant’s common stock, $0.001 par value, outstanding. 

DOCUMENTS INCORPORATED BY REFERENCE 

Part III  of  this  Annual  Report  on  Form  10-K  incorporates  information  by  reference  to  portions  of  the  definitive  proxy  statement  for  the  Company’s  Annual 
Meeting of Stockholders to be held in 2016, to be filed within 120 days of the registrant’s fiscal year ended December 31, 2015. 

EXPLANATORY NOTE 

The Company meets the "accelerated filer" requirements as of the end of its 2015 fiscal year pursuant to Rule 12b-2 of the Securities Exchange Act of 1934, as
amended.  However,  pursuant  to  Rule 12b-2  and  SEC  Release  No. 33-8876,  the  Company  (as  a  smaller reporting  company  transitioning  to  the  larger  reporting
company system based on its public float as of June 30, 2015) is not required to satisfy the larger reporting company requirements until its first quarterly report on
Form 10-Q for the 2016 fiscal year and thus remains eligible to use the scaled disclosure requirements applicable to smaller reporting companies under Item 10 of
Regulation S-K under the Securities Act of 1933, as amended, in this Annual Report on Form 10-K.

  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
   
  
  
  
  
  
  
 
 
 
 
 
ASSEMBLY BIOSCIENCES, INC. 
TABLE OF CONTENTS 

Business
Risk Factors
Unresolved Staff Comments
Properties
Legal Proceedings
Mine Safety Disclosures

PART I
Item 1.
Item 1A.
Item 1B.
Item 2.
Item 3.
Item 4.
PART II
Item 5.
Item 6.
Item 7.
Item 7A.
Item 8.
Item 9.
Item 9A.
Item 9B.
PART III
Item 10.
Item 11.
Item 12.
Item 13.
Item 14.
Item 15.
Financial Statements

Market for Registrant’s Common Equity, Related Stockholder Matters and Issuer Purchases of Equity Securities
Selected Financial Data
Management’s Discussion and Analysis of Financial Condition and Results of Operation
Quantitative and Qualitative Disclosures about Market Risk
Financial Statements and Supplementary Data
Changes in and Disagreements with Accountants on Accounting and Financial Disclosure
Controls and Procedures
Other Information

Directors, Executive Officers and Corporate Governance
Executive Compensation
Security Ownership of Certain Beneficial Owners and Management and Related Stockholder Matters
Certain Relationships and Related Transactions, and Director Independence
Principal Accounting Fees and Services
Exhibits, Financial Statement Schedules

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F-1

  
  
  
 
References to Assembly Biosciences, Inc. 

Throughout this Annual Report on Form 10-K, the “Company,” “Assembly,” “we,” “us,” and “our,” except  where the context requires otherwise,
refer  to  Assembly  Biosciences,  Inc.  and  its  consolidated  subsidiary,  and  “our  board  of  directors”  refers  to  the  board  of  directors  of  Assembly 
Biosciences, Inc. 

Forward Looking Information 

This  Annual  Report  on  Form 10-K  contains  “forward-looking  statements”  within  the  meaning  of  Section  27A  of  the  Securities  Act  of  1933,  as
amended  (the  “Securities  Act”)  and  Section  21E  of  the  Securities  Exchange  Act  of  1934,  as  amended  (the  “Exchange  Act”).  Such  forward-looking 
statements  involve  substantial  risks  and  uncertainties.  All  statements,  other  than  statements  of  historical  facts,  contained  in  this  Annual  Report  on
Form 10-K,  including statements  regarding  our strategy, future  operations,  future  financial position,  future  revenues,  projected  costs,  prospects, plans
and  objectives  of  management,  are  forward-looking  statements.  The  words  “anticipate,”  “believe,”  “estimate,”  “expect,”  “intend,”  “may,”  “plan,”
“predict,”  “project,”  “target,”  “potential,”  “will,”  “would,”  “could,”  “should,”  “continue”  and  similar  expressions  are  intended  to  identify  forward-
looking statements, although not all forward-looking statements contain these identifying words. 

The forward-looking statements in this Annual Report on Form 10-K include, among other things, statements about: 

•
•
•
•
•
•
•
•
•
•
•
•

the initiation, timing, progress and results of pre-clinical studies and clinical trials, and our research and development programs;
our plans to develop and commercialize our product candidates;
our ability to establish and maintain collaborations;
our ability to obtain additional funding;
the timing or likelihood of regulatory filings and approvals;
the implementation of our business model, strategic plans for our business, product candidates and technology;
our commercialization, marketing and manufacturing capabilities and strategy;
the rate and degree of market acceptance and clinical utility of our products;
our competitive position;
our intellectual property position;
developments and projections relating to our competitors and our industry; and
our estimates regarding expenses, future revenue, capital requirements and needs for additional financing.

We  may  not  actually  achieve  the  plans,  intentions  or  expectations  disclosed  in  our  forward-looking  statements,  and  you  should  not  place  undue
reliance on our forward-looking statements. Actual results or events could differ materially from the plans, intentions and expectations disclosed in the
forward-looking statements we make. We have included important factors in the cautionary statements included in this Annual Report on Form 10-K, 
particularly in the “Risk Factors” section, that could cause actual results or events to differ materially from the forward-looking statements that we make. 
Our forward-looking statements do not reflect the potential impact of any future acquisitions, mergers, dispositions, joint ventures or investments we
may make. 

You should read this Annual Report on Form 10-K and the documents that we have filed as exhibits to this Annual Report on Form 10-K completely 
and with the understanding that our actual future results may be materially different from what we expect. We do not assume any obligation to update
any forward-looking statements, whether as a result of new information, future events or otherwise, except as required by law. 

  
  
  
  
  
  
  
  
  
 
 
 
 
 
 
 
 
 
 
 
 
PART I 

Item 1. Business 

Overview 

We are a biotechnology company advancing two innovative platform programs: a new class of oral therapeutics for the treatment of hepatitis B virus
(HBV) infection and novel class of oral biological therapeutics, which are designed to restore health to a dysbiotic microbiome. Our HBV-cure program 
is  aimed  at  increasing  the  current  low  cure  rates  for  patients  with  HBV  and  is  pursuing  several  drug  candidates  that  inhibit  multiple  viral  targets
throughout  the  HBV  lifecycle.  Assembly  has  discovered  several  novel  core  protein  Allosteric  Modulators  (CpAMs),  which  are  small  molecules  that
directly target and allosterically modulate a number of HBc functions. Our Microbiome Program consists of a fully integrated platform that includes a
robust strain identification and selection process, methods for strain isolation and growth under cGMP conditions, and a patent pending delivery system,
GEMICELTM,  which  allows  for  targeted  oral  delivery  of  live  biologic  and  conventional  therapies  to  the  lower  gastrointestinal,  or  GI  tract.  The  lead
program  from  this  platform,  AB-M101,  is  in  development  for  the  treatment  of  C.  difficile-  infections  (CDI).  Using  its  microbiome  platform,  the 
Company is developing additional product candidates. 

Business Strategy 

Assembly  is  currently  focused  on  advancing  science  and  enhancing  the  health  and  well-being  of  patients  with  hard-to-treat  infectious  diseases  by
building  a  world  class  company  focused  on  serious  conditions  that  are  inadequately  treated.  This  commitment  drives  our  efforts  to  forge  a  new  and
different  path  to  treating  these  conditions,  inspired  by  the  needs  of  millions  of  affected  patients  with  our  platform  technologies  that  may  have  the
potential  to  overcome  the  limitations  of  conventional  approaches.  We  have  two  promising  proprietary  technology  platforms:  a  portfolio  of  novel,
potentially curative CpAMs for HBV and a novel class of oral biological drugs designed to restore a dysbiotic microbiome. Both of these conditions
include substantial numbers of patients with intractable disease who cannot be cured by current therapies. We intend to progress these programs using a
variety  of  strategic  arrangements,  including  potentially  collaborations,  licenses,  partnerships  and  other  types  of  business  arrangements.  These  may
provide non-dilutive funding for drug development, as well as clinical development and commercial expertise. 

HBV-Cure Program 

The target of our HBV cure program is a clinical cure for hepatitis B virus, a pathogen that infects approximately 350 million people worldwide and
is associated with an estimated 650,000 deaths each year. Our HBV-Cure research team is working on discovering and developing multiple CpAMs with
the potential to modulate the HBV core protein – an essential polyfunctional viral protein - at multiple points in the viral lifecycle. 

HBc protein is involved in several steps of the HBV lifecycle and is essential for HBV’s continued regeneration and prolonged survival. Modulation
of HBc with Assembly’s CpAMs has demonstrated preclinical proof of principle. In multiple cell-based models CpAMs selectively reduced viral load, 
which is the amount of infectious viral particles released from infected cells and modulated closed circular covalent DNA (cccDNA), a special DNA 
structure  that  arises  in  the  cell  nucleus  of  some  viruses  and  is  associated  with  viral  persistence.  Different  CpAMs  appear  to  have  differentiated
mechanisms, giving us a potential pipeline of differentiated early-stage products. This enables us to pursue both monotherapy and combination therapies
that  include  molecules  with  differentiated  mechanisms,  potentially  facilitating  the  achievement  of  improved  cure  rates.  The  goal  is  to  eradicate  the
infection with an orally-administered regimen. We believe that Assembly is uniquely positioned to execute on this strategy, with a senior scientific team
that has over 30 years of combined experience working on HBV. 

Background 

Hepatitis B virus (HBV) is a chronic infectious disease of the liver. It is a leading global cause of chronic liver disease and liver transplants. The
World Health Organization estimates that nearly 350 million people worldwide, or approximately 6% of the world’s population, are infected with HBV.
An estimated 10 to 30 million people are newly infected each year and 650,000 people die annually from HBV-related liver disease. The Centers for
Disease  Control  and  Prevention  (CDC)  has  reported  that  almost  two  million  people  in  the  United  States  have  been  infected  with  HBV.  HBV  is  an
underappreciated global epidemic with twice as many people infected and with a higher rate of mortality and morbidity than hepatitis C virus and HIV
infections combined. While many HBV patients currently receive treatment, the majority do not, partly due to the lack of effective therapies. The cure
rate with current therapies is estimated at only 3-5%. Despite the low rate of diagnosis and treatment, the current market for HBV therapies is estimated
at $3.2 billion, with significant growth expected in the years ahead as more effective drugs are developed and launched. 

1  
  
  
  
  
  
  
  
 
  
  
  
  
Current Treatments 

Current therapeutic options for HBV include: 

·

·

Antiviral medications. Several antiviral medications - including lamivudine (Epivir®), adefovir (Hepsera®), telbivudine (Tyzeka®) and entecavir 
(Baraclude®)  –  effectively  reduce  circulating  virus.   Chronic  therapy  with  these  agents  results  in  reduced  liver  inflammation  and  fibrosis.
Unfortunately, viral replication resumes when therapy is stopped.

Interferon alfa-2b (Intron A). This synthetic version of a substance produced by the body to fight infection is used mainly for young people
with hepatitis B who don't want to undergo long-term treatment or who might want to become pregnant within a few years. It is administered by
injection. Side effects may include flu-like symptoms and depression.

Our HBV Focus: Leveraging HBV Core Protein to Achieve a Cure using Core Protein Allosteric Modulators (CpAMs) 

HBV is a DNA-virus that establishes an intra-nuclear reservoir of cccDNA, which sustains infection in the liver through chronic and occult hepatitis
B infection. No current oral therapies can target cccDNA activity directly, and thus orally available molecules that can modulate cccDNA are highly
sought in the HBV field. A key focus of Assembly’s cure effort is the HBV Core protein (HBc), a highly conserved viral structural protein that has no
human homologue and is involved in numerous aspects of the HBV lifecycle, including interaction with the viral cccDNA. Assembly has discovered
multiple novel series of CpAMs, which are small molecules that directly target and allosterically modulate a number of HBc functions. Assembly’s HBV 
pipeline therefore offers the potential for both first in class and best in class opportunities for developing agents that target multiple aspects of the viral
lifecycle, such as HBc/cccDNA interactions. We believe that our approaches to targeting multiple aspects of HBc provide a promising foundation for
developing a clinical cure for HBV. 

To successfully eradicate HBV infections, researchers need to address both the “downstream” inhibition of HBV viral replication and the “upstream”
part of the lifecycle that reflects cccDNA activity. We define downstream inhibition of the HBV lifecycle as targeting HBV from the point of formation
of the viral capsid to the release of viral particles from the cell for re-infection. We believe that our ability to develop CpAMs that target multiple aspects
of the viral lifecycle may allow us to develop combination regimens that achieve better cure rates by targeting both target “upstream” and “downstream”
components. Other core competencies and competitive advantages we bring to our lead program include our knowledge of HBV biology, our proprietary
enabling assays, our highly experienced chemistry and our relevant clinical expertise. 

A clinically and preclinically accepted benchmark for therapeutic agents aiming to affect cccDNA activity is the level of expression of several key
viral  antigens.  On  this  basis,  Assembly’s  CpAMs  have  shown  preclinical  proof  of  principle.  In  a  variety  of  cell  culture  models,  CpAMs  have
demonstrated  the  ability  to  reduce  production  of  viral  antigens:  HBV  E  antigen  (HBeAg)  and  HBV  S  antigen  (HBsAg).  Sustained  (post  treatment)
inhibition of HBsAg in patients is considered a functional cure, and is a key endpoint for clinical development. 

Our clinical strategy encompasses testing CpAMs as monotherapy and in combination. Our access to multiple classes of CpAMs allows us to explore
their activity in combination across CpAM classes and with other classes of HBV therapies. Our planned clinical program will start with Phase I (safety)
studies  of  CpAMs  as  single  agents  in  healthy  volunteers.  Phase  IB  studies  in  patients  would  include  assessments  of  CpAMs  as  single  agents  and  in
combination with approved therapies for HBV. The Phase II studies would explore duration of therapy in dose finding single agent and combination
studies across CpAM classes and with other classes of therapy. 

Assembly is planning to complete its preclinical studies in 2016. Clinical trials with its first lead molecule are planned to commence in the second
half  of  2016.  Assembly’s  CpAM  platform  provides  opportunities  to  create  a  pipeline  encompassing  multiple  generations  of  antiviral  drugs,  and
Assembly plans to advance second and third generation CpAMs into clinical development in 2017. Assembly also has research programs assessing other
novel targets for HBV that are complementary to our programs focusing on Core protein. 

License Agreement and Intellectual Property 

On September 3, 2013, we entered into a license agreement (the “IURTC License Agreement”) with Indiana University Research and Technology
Corporation (“IURTC”) to acquire the rights to develop and commercialize products associated with multiple patent applications covering aspects of our
HBV program held by IURTC. The licensed intellectual property includes platform patent applications covering aspects of HBc, our novel mechanisms
of  action,  methods  of  treatment  and  the  novel  drug  development  assays  our  team  is  creating.  As  part  of  this  agreement,  we  are  obligated  to  make
milestone payments based upon the successful accomplishment of clinical and regulatory milestones. The total amount of all potential future milestone
payments at the end of 2015 was $825,000. None of the criteria for these milestones have yet been met. Under the IURTC License Agreement, we are
also obligated to pay IURTC royalty payments based on net sales of the licensed technology ranging from 0.5% to 1.75%. 

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In addition, the IURTC License Agreement requires an annual diligence maintenance fee as follows: 

2015
2016 through the year in which first commercial sale occurs
Year following first commercial sale and all subsequent years

  $
  $
  $

50,000
75,000
100,000

We  also  have  filed  composition  of  matter  patent  applications  for  our  novel  CpAM  agents.  We  expect  to  file  additional  patent  applications  going

forward. 

Microbiome Program Platform  

Background 

Our  Microbiome  Program  is based  on the  oral targeted delivery  of novel microbiome-based  therapies  in  a patent  pending  oral formulation,  called
Gemicel™  applying  our novel  coating  and  encapsulation  technology  that  allows  for  targeted  delivery  of  complex  agents  to  select  regions  of  the
gastrointestinal,  or  GI,  tract.  Using  this  proprietary  delivery  platform,  we  aim  to  deliver  selected  combinations  of  monoculture  strains  of  beneficial
bacteria  in  novel  “synthetic  formats”  to  the  GI  tract. Our  first  indication  is  recalcitrant  CDI,  and  we  plan  to  leverage  our  Microbiome  program  into
multiple  other  areas  of  high  relevance  to  gut  microbiome  disorders  including  other  infectious  disease  and  GI  disorders,  indications  in  Oncology,
Diabetes, Obesity, other Metabolic disorders, and CNS disorders. 

Our  approach  builds  upon  experience  reported  in  the  literature  of  successfully  treating  CDI  and  other  disease  indications  with  fecal  material
transplants  or  FMT,  and  seeks  to  provide  a  potentially  curative  therapy  using  a  far  more  “drug  like”  approach  that  delivers  targeted  and  specific 
microbiome therapies in an oral capsule. 

CDI is our first indication. In recent years, there has been increasing interest in the therapeutic potential of the human microbiome - the billions of
microbes  living  in  and  on  people  -  to  impact  health  and  disease.  An  early  and  obvious  target  was  CDI,  the  most  common  nosocomial,  or  hospital
acquired,  infection,  which  has  become  a  significant  medical  problem  in  hospitals  and  long-term  care  facilities  as  it  becomes  increasingly  resistant  to 
common antibiotics. CDI is estimated to afflict more than 500,000 people each year in the US alone. It is a serious illness resulting from infection of the
inner lining of the colon by C. difficile bacteria, which produce toxins that cause inflammation, severe diarrhea and, in the most serious cases, death. 
Certain  subpopulations,  such  as  older  patients,  transplant  patients,  patients  taking  concomitant  antibiotics  and  cancer  patients,  are  at  a  higher  risk  of
contracting  CDI.  Patients  typically  develop  CDI  from  the  use  of  broad-spectrum  antibiotics  that  disrupt  normal  gastrointestinal  (gut)  flora,  thus
allowing C.  difficile  bacteria  to  flourish  unchecked  and  produce  toxins.  C.  difficile  is  a  spore-forming  bacterium.  Spores  released  into  the  hospital 
environment by patients with active disease can survive for months on dry surfaces in hospital rooms such as beds and doors. It also spreads when spores
from other patients are transmitted on the hands and clothing of healthcare workers. 

Current Treatments 

Current therapeutic options for CDI include fidaxomicin, oral vancomycin and the off-label use of metronidazole. However, approximately 35% of
patients initially treated with these drugs either fail to respond or do not achieve a sustained response. About 50% of initially non-responsive patients fail 
to  achieve  a  sustained  response  from  second  and  third  line  treatment.  Because  of  the  difficulties  in  achieving  a  sustained  response  to  treatment,  we
estimate that, in the U.S. alone, there are more than 800,000 treatments per year for CDI. 

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Microbiome Therapies May Represent an Effective New Treatment Option for CDI 

There has been considerable experience reported in the literature of successfully treating CDI with FMT from healthy individuals. FMT is believed to
act by restoring a healthy balance of microbes in the gut. Despite its clinical efficacy, broad acceptance of FMT has been problematic, because of the
possibility  of  unknown  and  potentially  damaging  constituents  in  human  derived  materials.  Other  options  have  been  sought.  Preliminary  CDI  studies
using selected bacterial strains or bacterial spores from processed FMT have been promising. These reports have demonstrated a significant and growing
precedent  of  successful  cures  in  patients  who  had  failed  all  prior  treatment,  and  provide  a  path  to  potentially  curative  therapy  using  a  targeted  and
specific microbiome therapy-one that can achieve the therapeutic benefits of FMT but in a form that is more predictable, safe and drug-like. 

Proof-of-concept for this approach was demonstrated using a preparation of fecal material from normal donors that contained only bacterial spores. In
a U.S. Phase IB study, a single oral dose of spores administered in multiple capsules produced a 90% sustained response in 30 CDI patients who had
failed  three  prior  regimens.  In  addition,  the  carriage  of  antibiotic  resistant  bacteria  substantially  declined  in  these  patients.  In  another  small  study,  a
selection of 32 specific viral strains achieved a sustained response in two elderly patients with chronic refractory CDI. 

The concept has also been validated in animal studies. Several recent publications have demonstrated that administering even a few strains of bacteria
may  be  sufficient  to  have  a  curative  effect  in  mouse  models  of  CDI,  and  one  study  suggested  that  even  a  single  strain  can  be  effective.  In  addition,
testable mechanisms of how commensal bacteria may inhibit the growth and persistence of C. difficile have been reported or postulated. 

Our  CDI  program  is  based  on  the  premise  that  an  oral  capsule  containing  specific  bacteria  grown  in  monoculture  and  manufactured  under
pharmaceutical-like GMP conditions (in effect a ‘synthetic’ biologic product), has the potential to provide the therapeutic benefits of FMT therapy in a
form that is economically viable and scalable for use in first line, as well as in second and third line treatment. In contrast, the commercial and clinical
provision of whole or processed feces would require the provision of, or the purification and/or extraction from, human donor material, and as such is
highly unlikely to be considered feasible except for a relatively small number of refractory CDI patients who have failed antibiotic treatment at least
three times. 

However, the development of a ‘synthetic’ bacterial product for the treatment of CDI, while promising, presents several basic challenges. 

The first challenge is the selection of bacterial strains likely to be effective. We believe that our ongoing bacterial discovery program enhances the
probability that we will identify strains that will be effective in humans. This program involves collaboration with leading academic medical centers with
relevant expertise, and includes special methods of identifying colonic strains from CDI patients receiving FMT, along with bioinformatic assessment of
relevant data from resolved patients, and the isolation, culture, and screening of promising strains. 

A  second  challenge  is  the  effective  and  reliable  oral  delivery  of  sufficient  of  organisms  to  the  colon  using  a  minimal  number  of  capsules.  To
accomplish  this,  we  in-licensed  a  delivery  technology  we  call  GemicelTM.  Gemicel  is  a  novel  coating  and  encapsulation  technology  that  allows
controlled  delivery  of  an  oral  formulation  specifically  designated  to  achieve  targeted  fpulsed  release  to  selected  portions  of  the  GI  system  tract  by
leveraging differences in their pH environments. 

We have demonstrated that our coating technology, which can be applied at body temperature ranges under aerobic or anaerobic conditions, does not
cause any loss of a wide range of viable microorganisms. We have also demonstrated in vitro that, under conditions commonly accepted as representing 
conditions in each section of the GI tract lumen, the formulation can deliver its contents to the targeted sites. The results of a clinical scintigraphy study
of Gemicel in healthy volunteers conducted in 2015 demonstrated that Gemicel can effectively release a bolus therapeutic payload at specific locations
in the lower GI tract, including the ileum and ascending colon, two locations especially relevant for the treatment of C. difficile pathogens. The data were 
generated in three clinical cohorts that used radioisotope-based scintigraphy to precisely image the drug delivery properties of Gemicel. 

A third challenge is achieving successful process, scale up, and reliable and economic manufacture of the strains we select for use in CDI therapy for
clinical  trials  and,  ultimately,  commercialization.  The  Assembly  team  has  considerable  experience  in  industrial  scale  production  of  bacteria  under
pharmaceutical-like Good Manufacturing Practice (GMP) requirements, and there are several commercial scale vendors we have identified to facilitate
this activity. However certain bacteria can be very difficult to freeze-dry (lyophilize) for encapsulation, and some can be very difficult to grow at a large
scale. We believe that it is feasible to mitigate both clinical, regulatory and manufacturing risk by carefully selecting strains for clinical development that
are  effective  in  our  preclinical  assessments,  that  do  not  carry  antibiotic  resistance  or  virulence  genes,  and  that  can  be  lyophilized  and  grown  at  scale
under standard anaerobic and/or aerobic conditions as required. 

We anticipate that our clinical development program for CDI using systematically selected, bacterial strains will encompass both first line as well as
second and third line treatment. We expect that our first clinical trial will be in CDI patients who have relapsed after two or three standard antibiotic
regimens. We will explore various regimens for further clinical development in these initial studies. We plan to complete our IND enabling studies in
2016 and then initiate Phase Ib study for our lead product candidate AB-M101, in the second half of 2016.  

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Depending on the progress and success of our CDI program we may decide to further leverage our discovery program and formulation technology to
pursue  other  microbiome-related  indications  such  as  inflammatory  bowel  disease,  irritable  bowel  syndrome,  and  metabolic  diseases,  as  new  data
becomes available clarifying the relationship of the gut microbiome to these conditions. 

License Agreement and Intellectual Property 

On November 8, 2013, we entered into a License and Collaboration Agreement with Therabiome, LLC, for all intellectual property and know-how
owned or controlled by Therabiome relating to the oral delivery of pharmaceutical drugs to specific sites in the intestine, using a pH sensitive release
platform  technology.  Under  the  agreement,  Therabiome  granted  Assembly  the  exclusive  worldwide  license,  with  rights  to  sublicense,  to  develop  the
intellectual property for commercialization (a) in the use of bacteria, complex proteins, viral antigens and small molecules by oral delivery in (i) gastro-
intestinal dysbiosis, including but not limited to C. difficile infections, irritable bowel syndrome-constipation and inflammatory bowel disease, (ii) auto-
immune  disorders  and  autism,  including  but  not  limited  to  as  controlled  by  bacteria  or  virus,  and  (iii)  orally  delivered  vaccines,  including  viral  and
bacterial,  and  (b)  any  oral  delivery  of  small  molecules  using  the  licensed  intellectual  property.     We  will  be  solely  responsible  for  all  research  and
development activities with respect to any product we develop under the license. 

For the license, we paid Therabiome an upfront non-refundable license fee of $300,000.  In September 2014, we paid Therabiome $100,000 upon the
occurrence of the first proof of principle for a bacteria strain. We will be required to pay an additional $100,000 upon the occurrence of the proof of
principle  for  a  virus.  We  must  pay  Therabiome  clinical  and  regulatory  milestones  for  each  product  or  therapy  advanced  from  the  platform,  for  U.S.
regulatory milestones, depending on whether the milestone occurs before the filing of the first new drug application, or NDA, for a product or after the
first, second or third NDA filings, as follows: 

Regulatory and Clinical Milestones

Upon the filing of an IND with the FDA:

First dose first patient - Phase I Clinical Trial

First dose first patient - Phase II Clinical Trial

First dose first patient - Phase III Clinical Trial

  $100,000 -  $130,000

  $250,000 -  $325,000

  $500,000 -  $650,000

  $750,000 -  $975,000

Upon filing of an NDA or BLA with the FDA

  $1,000,000 - $1,300,000

Upon marketing approval by the FDA

Upon approval of a supplemental NDA (sNDA) for a new Indication, in the U.S

  $3,000,000

  $1,000,000

We also must pay Therabiome lesser amounts for foreign regulatory milestones, which vary by country and region, and which depend on whether the
milestone occurs before the filing of the first NDA filing or after the first, second or third NDA filings. These payments will be: one-third of the U.S. 
milestones paid upon a foreign equivalent of an investigational new drug application, or IND and marketing approval for each product in the European
Union or Japan; 10% of the U.S. milestones paid upon a foreign equivalent of an IND and marketing approval for each product in China; 10% of the
U.S. milestone paid upon marketing approval for each product in India and Brazil; and 1% of the U.S. milestone paid upon marketing approval for each
product in all other countries. We also must pay Therabiome royalties on annual net product sales in the low to mid-single digit percentages plus, once
annual net sales exceed two specified thresholds, a one-time cash payment upon reaching each threshold.  

Government Regulation 

Government authorities in the U.S., at the federal, state and local level, and in other countries extensively regulate, among other things, the research,
development,  testing,  manufacture,  including  any  manufacturing  changes,  packaging,  storage,  recordkeeping,  labeling,  advertising,  promotion,
distribution, marketing, post-approval monitoring and reporting, import and export of pharmaceutical products, such as those we are developing. 

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U.S. drug approval process 

In  the  U.S,  the  FDA  regulates  drugs  under  the  Federal  Food,  Drug,  and  Cosmetic  Act,  or  FDCA,  and  implementing  regulations.  The  process  of
obtaining  regulatory  approvals  and  the  subsequent  compliance  with  appropriate  federal,  state,  local  and  foreign  statutes  and  regulations  requires  the
expenditure  of  substantial  time  and  financial  resources.  Failure  to  comply  with  the  applicable  U.S.  requirements  at  any  time  during  the  product
development process, approval process or after approval, may subject an applicant to a variety of administrative or judicial sanctions, such as the FDA’s 
refusal to approve pending applications, withdrawal of an approval, imposition of a clinical hold, issuance of warning letters and untitled letters, product
recalls,  product  seizures,  total  or  partial  suspension  of  production  or  distribution  injunctions,  fines,  refusals  of  government  contracts,  restitution,
disgorgement of profits or civil or criminal penalties. 

The process required by the FDA before a drug may be marketed in the U.S. generally involves the following: 

•

•

•
•

•
•

•

completion of preclinical laboratory tests, animal studies and formulation studies in compliance with the FDA’s good laboratory practice, 
or GLP, regulations;
submission to the FDA of an investigational new drug application, or IND, which must become effective before human clinical trials may
begin;
approval by an independent institutional review board, or IRB, at each clinical site before each trial may be initiated;
performance  of  adequate  and  well-controlled  human  clinical  trials  in  accordance  with  good  clinical  practices,  or  GCP,  to  establish  the
safety and efficacy of the proposed drug for each indication;
submission to the FDA of a new drug application, or NDA;
satisfactory  completion  of  an  FDA  inspection  of  the  manufacturing  facility  or  facilities  at  which  the  product  is  produced  to  assess
compliance with current good manufacturing practices, or cGMP, requirements and to assure that the facilities, methods and controls are
adequate to preserve the drug’s identity, strength, quality and purity; and
FDA review and approval of the NDA.

Preclinical Studies and IND 

Preclinical studies include laboratory evaluation of product chemistry and formulation, as well as in vitro and animal studies to assess the potential
for adverse events and in some cases to establish a rationale for therapeutic use. The conduct of preclinical studies is subject to federal regulations and
requirements, including  GLP  regulations  for  safety/toxicology  studies.  An  IND sponsor  must  submit  the  results  of  the  preclinical  tests,  together with
manufacturing information, analytical data, any available clinical data or literature and plans for clinical studies, among other things, to the FDA as part
of  an  Investigational  New  Drug  application  (IND).  Some  long-term  preclinical  testing,  such  as  animal  tests  of  reproductive  adverse  events  and
carcinogenicity, may continue after the IND is submitted. An IND automatically becomes effective 30 days after receipt by the FDA, unless before that
time the FDA raises concerns or questions related to one or more proposed clinical trials and places the trial on clinical hold. In such a case, the IND
sponsor and the FDA must resolve any outstanding concerns before the clinical trial can begin. As a result, submission of an IND may not result in the
FDA allowing clinical trials to commence. 

Clinical trials 

Clinical  trials  involve  the  administration  of  the  investigational  new  drug  to  human  subjects  under  the  supervision  of  qualified  investigators  in
accordance  with  GCP  requirements,  which  include,  among  other  things,  the  requirement  that  all  research  subjects  provide  their  informed  consent  in
writing  before  their  participation  in  any  clinical  trial.  Clinical  trials  are  conducted  under  written  study  protocols  detailing,  among  other  things,  the
objectives of the study, the parameters to be used in monitoring safety and the effectiveness criteria to be evaluated. A protocol for each clinical trial and
any  subsequent  protocol  amendments  must  be  submitted  to  the  FDA  as  part  of  the  IND.  In  addition,  an  IRB  at  each  institution  participating  in  the
clinical trial must review and approve the plan for any clinical trial before it commences at that institution, and the IRB must conduct continuing review.
The IRB must review and approve, among other things, the study protocol and informed consent information to be provided to study subjects. An IRB
must operate in compliance with FDA regulations. Information about certain clinical trials must be submitted within specific timeframes to the National
Institutes of Health for public dissemination at www.clinicaltrials.gov. 

Human clinical trials are typically conducted in three sequential phases, which may overlap or be combined: 

•

•

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Phase 1: The drug is initially introduced into healthy human subjects or patients with the target disease or condition and tested for safety,
dosage tolerance, absorption, metabolism, distribution, excretion and, if possible, to gain an early indication of its effectiveness.
Phase  2: The  drug  is  administered  to  a  limited  patient  population  to  identify  possible  adverse  effects  and  safety  risks,  to  preliminarily
evaluate the efficacy of the product for specific targeted diseases and to determine dosage tolerance and optimal dosage.
Phase 3: The drug is administered to an expanded patient population in adequate and well-controlled clinical trials to generate sufficient 
data to statistically confirm the efficacy and safety of the product for approval, to establish the overall risk-benefit profile of the product 
and to provide adequate information for the labeling of the product.

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Progress reports detailing the results of the clinical trials must be submitted at least annually to the FDA. Additionally, IND safety reports must be
submitted for serious and unexpected suspected adverse reactions, findings from animal or in vitro testing or other studies that suggest a significant risk 
in  humans,  and  any  clinically  important  increase  in  the  rate  of  a  serious  suspected  adverse  reaction  over  that  listed  in  the  protocol  or  investigator
brochure. Phase 1, Phase 2 and Phase 3 clinical trials may not be completed successfully within any specified period, or at all. Furthermore, the FDA or
the sponsor may suspend or terminate a clinical trial at any time on various grounds, including a finding that the research subjects are being exposed to
an  unacceptable  health  risk.  Similarly,  an  IRB  can  suspend  or  terminate  approval  of  a  clinical  trial  at  its  institution  if  the  clinical  trial  is  not  being
conducted in accordance with the IRB’s requirements or if the drug has been associated with unexpected serious harm to patients. 

Marketing approval 

Assuming  successful  completion  of  the  required  clinical  testing,  the  results  of  the  preclinical  studies  and  clinical  trials,  together  with  detailed
information relating to the product’s chemistry, manufacture, controls and proposed labeling, among other things, are submitted to the FDA as part of an
NDA requesting approval to market the product for one or more indications. Under federal law, the submission of most NDAs is additionally subject to a
substantial application user fee, currently $2,374,200 and the sponsor of an approved NDA is also subject to annual product and establishment user fees,
currently exceeding $114,450 per product and $585,200 per establishment. These fees are typically increased annually. 

The FDA conducts a preliminary review of all NDAs within the first 60 days after submission before accepting them for filing to determine whether
they are sufficiently complete to permit substantive review. The FDA may request additional information rather than accept an NDA for filing. In this
event, the application must be resubmitted with the additional information. The resubmitted application is also subject to review before the FDA accepts
it for filing. Once the submission is accepted for filing, the FDA begins an in-depth substantive review. The FDA has agreed to specified performance
goals in the review of NDAs. Under these goals, the FDA has committed to review most applications for non-priority products within 10 months, and
most applications for priority review products, that is, drugs that the FDA determines represent a significant improvement over existing therapy, within
six  months.  The  review  process  may  be  extended  by  the  FDA  for  three  additional  months  to  consider  certain  information  or  clarification  regarding
information  already  provided  in  the  submission.  The  FDA  may  also  refer  applications  for  novel  drugs  or  products  that  present  difficult  questions  of
safety or efficacy to an advisory committee, typically a panel that includes clinicians and other experts, for review, evaluation and a recommendation as
to  whether  the  application  should  be  approved.  The  FDA  is  not  bound  by  the  recommendations  of  an  advisory  committee,  but  it  considers  such
recommendations carefully when making decisions. 

Before approving an NDA, the FDA typically will inspect the facility or facilities where the product is manufactured. The FDA will not approve an
application  unless  it  determines  that  the  manufacturing  processes  and  facilities  are  in  compliance  with  cGMP  requirements  and  adequate  to  assure
consistent production of the product within required specifications. In addition, before approving an NDA, the FDA will typically inspect one or more
clinical sites to assure compliance with GCP and integrity of the clinical data submitted. 

The  testing  and  approval  process  requires  substantial  time,  effort  and  financial  resources,  and  each  trial  may  take  many  years  to  complete.  Data
obtained  from  clinical  activities  are  not  always  conclusive  and  may  be  susceptible  to  varying  interpretations,  which  could  delay,  limit  or  prevent
regulatory approval. The FDA may not grant approval on a timely basis, or at all. We may encounter difficulties or unanticipated costs in our efforts to
develop our product candidates and secure necessary governmental approvals, which could delay or preclude us from marketing our products. 

After the FDA’s evaluation of the NDA and inspection of the manufacturing facilities, the FDA may issue an approval letter or a complete response
letter.  An  approval  letter  authorizes  commercial  marketing  of  the  drug  with  specific  prescribing  information  for  specific  indications.  A  complete
response letter generally outlines the deficiencies in the submission and may require substantial additional testing or information in order for the FDA to
reconsider the application. If and when those deficiencies have been addressed to the FDA’s satisfaction in a resubmission of the NDA, the FDA will 
issue an approval letter. The FDA has committed to reviewing such resubmissions in two or six months depending on the type of information included.
Even  with  submission  of  this  additional  information,  the  FDA  ultimately  may  decide  that  the  application  does  not  satisfy  the  regulatory  criteria  for
approval and refuse to approve the NDA. Even if the FDA approves a product, it may limit the approved indications for use for the product, require that
contraindications, warnings or precautions be  included  in  the product  labeling,  require  that  post-approval studies, including Phase 4 clinical trials, be 
conducted to further assess a drug’s safety after approval, require testing and surveillance programs to monitor the product after commercialization, or
impose other conditions, including distribution restrictions or other risk management mechanisms, including Risk Evaluation and Mitigation Strategies,
or REMs, which can materially affect the potential market and profitability of the product. The FDA may prevent or limit further marketing of a product
based on the results of post-market studies or surveillance programs. After approval, some types of changes to the approved product, such as adding new
indications, manufacturing changes and additional labeling claims, are subject to further testing requirements and FDA review and approval. 

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Fast track designation 

The FDA is required to facilitate and expedite the development and review of drugs that are intended for the treatment of serious or life-threatening
condition for which there is no effective treatment and which demonstrate the potential to address the unmet medical needs for the condition. Under the
fast track program, the sponsor of a new drug candidate may request the FDA to designate the product for a specific indication as a fast track product
concurrent  with  or  after  the  filing  of  the  IND  for  the  product  candidate.  The  FDA  must  determine  if  the  product  candidate  qualifies  for  fast  track
designation within 60 days after receipt of the sponsor’s request. 

In addition to other benefits, such as the ability to use surrogate endpoints and have greater interactions with the FDA, the FDA may initiate review
of  sections  of  a  fast  track  product’s  NDA  before  the  application  is  complete.  This  rolling  review  is  available  if  the  applicant  provides  and  the  FDA
approves a schedule for the submission of the remaining information and the applicant pays applicable user fees. However, the FDA’s time period goal 
for  reviewing  a  fast  track  application  does  not  begin  until  the  last  section  of  the  NDA  is  submitted.  In  addition,  the  fast  track  designation  may  be
withdrawn by the FDA if the FDA believes that the designation is no longer supported by data emerging in the clinical trial process. 

Priority review 

Under  FDA  policies,  a  product  candidate  may  be  eligible  for  priority  review,  a  review  generally  within  a  six-month  time  frame  from  the  time  a
complete application is received. Products regulated by the FDA’s Center for Drug Evaluation and Research, or CDER, are eligible for priority review if
they are intended for treatment of a serious or life-threatening condition and provide a significant improvement compared to marketed products in the
treatment, diagnosis or prevention of a disease. A fast track designated product candidate would ordinarily meet the FDA’s criteria for priority review. 

Accelerated approval 

Under the FDA’s  accelerated  approval regulations,  the  FDA may  approve a  drug for a serious  or life-threatening illness that provides  meaningful
therapeutic benefit to patients over existing treatments based upon a surrogate endpoint that is reasonably likely to predict clinical benefit. In clinical
trials, a surrogate endpoint is a measurement of laboratory or clinical signs of a disease or condition that substitutes for a direct measurement of how a
patient feels, functions or survives. Surrogate endpoints can often be measured more easily or more rapidly than clinical endpoints. A product candidate
approved on this basis is subject to rigorous post-marketing compliance requirements, including the completion of Phase 4 or post-approval clinical trials 
to  confirm  the  effect  on  the  clinical  endpoint.  Failure  to  conduct  required  post-approval  studies,  or  confirm  a  clinical  benefit  during  post-marketing 
studies,  would  allow  the  FDA  to  withdraw  the  drug  from  the  market  on  an  expedited  basis.  All  promotional  materials  for  drug  candidates  approved
under accelerated regulations are subject to prior review by the FDA. 

Breakthrough therapy designation 

Under  the  provisions  of  the  new  Food  and  Drug  Administration  Safety  and  Innovation  Act,  or  FDASIA,  enacted  in  2012,  a  sponsor  can  request
designation of a product candidate as a “breakthrough therapy.” A breakthrough therapy is defined as a drug that is intended, alone or in combination
with one or more other drugs, to treat a serious or life-threatening disease or condition, and preliminary clinical evidence indicates that the drug may
demonstrate  substantial  improvement  over  existing  therapies  on  one  or  more  clinically  significant  endpoints,  such  as  substantial  treatment  effects
observed early in clinical development. Drugs designated as breakthrough therapies also may be eligible for priority review. The FDA must take certain
actions,  such  as  holding  timely  meetings  and  providing  advice,  intended  to  expedite  the  development  and  review  of  an  application  for  approval  of  a
breakthrough therapy. Even if a product qualifies for one or more of these programs, the FDA may later decide that the product no longer meets the
conditions for qualification or decide that the time period for FDA review or approval will not be shortened. 

Orphan drugs 

Under the Orphan Drug Act, the FDA may grant orphan drug designation to drugs intended to treat a rare disease or condition, which is generally
defined as a disease or condition that affects fewer than 200,000 individuals in the U.S. Orphan drug designation must be requested before submitting an
NDA. After the FDA grants orphan drug designation, the generic identity of the drug and its potential orphan use are disclosed publicly by the FDA.
Orphan  drug  designation  does  not  convey  any  advantage  in,  or  shorten  the  duration  of,  the  regulatory  review  and  approval  process.  The  first  NDA
applicant to receive FDA approval for a particular active ingredient to treat a particular disease with FDA orphan drug designation is entitled to a seven-
year exclusive marketing period in the U.S. for that product and indication. During the seven-year exclusivity period, the FDA may not approve any 
other applications to market the same drug for the same orphan indication, except in limited circumstances, such as a showing of clinical superiority to
the  product  with  orphan  drug  exclusivity  —  if  it  is  shown  to  be  safer,  more  effective  or  makes  a  major  contribution  to  patient  care.  Orphan  drug
exclusivity  does  not  prevent  the  FDA  from  approving  a  different  drug  for  the  same  disease  or  condition,  or  the  same  drug  for  a  different  disease  or
condition. Among the other benefits of orphan drug designation are tax credits for certain research and a waiver of the NDA application user fee. 

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Pediatric information 

Under the Pediatric Research Equity Act of 2003, an NDA or supplement to an NDA must contain data that are adequate to assess the safety and
effectiveness of the drug for the claimed indications in all relevant pediatric subpopulations, and to support dosing and administration for each pediatric
subpopulation  for  which  the  product  is  safe  and  effective.  The  FDA  may,  on  its  own  initiative  or  at  the  request  of  the  applicant,  grant  deferrals  for
submission  of  some  or  all  pediatric  data  until  after  approval  of  the  product  for  use  in  adults,  or  full  or  partial  waivers  from  the  pediatric  data
requirements. A sponsor of a new drug also is required to submit to the FDA a pediatric study plan generally 60 days after an end-of-Phase 2 meeting 
with the agency. Unless otherwise required by regulation, the pediatric data requirements do not apply to products with orphan drug designation. 

Combination products 

The  FDA  regulates  combinations  of  products  that  cross  FDA  centers,  such  as  drug,  biologic  or  medical  device  components  that  are  physically,
chemically or otherwise combined into a single entity, as a combination product. The FDA center with primary jurisdiction for the combination product
will take the lead in the premarket review of the product, with the other center consulting or collaborating with the lead center. 

The FDA’s Office of Combination Products, or OCP, determines which center will have primary jurisdiction for the combination product based on
the  combination  product’s  “primary  mode  of  action.”  A  mode  of  action  is  the  means  by  which  a  product  achieves  an  intended  therapeutic  effect  or
action. The primary mode of action is the mode of action that provides the most important therapeutic action of the combination product, or the mode of
action expected to make the greatest contribution to the overall intended therapeutic effects of the combination product. 

Often it is difficult for the OCP to determine with reasonable certainty the most important therapeutic action of the combination product. In those
difficult cases, the OCP will consider consistency with other combination products raising similar types of safety and effectiveness questions, or which
center has the most expertise to evaluate the most significant safety and effectiveness questions raised by the combination product. 

A sponsor may use a voluntary formal process, known as a Request for Designation, when the product classification is unclear or in dispute, to obtain
a  binding  decision  as  to  which  center  will  regulate  the  combination  product.  If  the  sponsor  objects  to  that  decision,  it  may  request  that  the  agency
reconsider that decision. 

Other regulatory requirements 

Any drug manufactured or distributed by us pursuant to FDA approvals will be subject to pervasive and continuing regulation by the FDA, including,
among  other  things,  requirements  relating  to  recordkeeping,  periodic  reporting,  product  sampling  and  distribution,  advertising  and  promotion  and
reporting  of  adverse  experiences  with  the  product.  After  approval,  most  changes  to  the  approved  product,  such  as  adding  new  indications  or  other
labeling claims are subject to prior FDA review and approval. 

The FDA may impose a number of post-approval requirements, including REMs, as a condition of approval of an NDA. For example, the FDA may
require post-marketing testing, including Phase 4 clinical trials, and surveillance to further assess and monitor the product’s safety and effectiveness after 
commercialization. 

In  addition,  drug  manufacturers  and  other  entities  involved  in  the  manufacture  and  distribution  of  approved  drugs  are  required  to  register  their
establishments  with  the  FDA  and  state  agencies,  and  are  subject  to  periodic  unannounced  inspections  by  the  FDA  and  these  state  agencies  for
compliance with cGMP requirements. Changes to the manufacturing process are strictly regulated and often require prior FDA approval before being
implemented.  FDA  regulations  also  require  investigation  and  correction  of  any  deviations  from  cGMP  and  impose  reporting  and  documentation
requirements upon us and any third-party manufacturers that we may decide to use. Accordingly, manufacturers must continue to expend time, money
and effort in the areas of production and quality control to maintain cGMP compliance. 

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Once an approval is granted, the FDA may withdraw the approval if compliance with regulatory requirements and standards is not maintained or if
problems  occur  after  the  product  reaches  the  market.  Later  discovery  of  previously  unknown  problems  with  a  product,  including  adverse  events  of
unanticipated severity or frequency, or with manufacturing processes, or failure to comply with regulatory requirements, may result in revisions to the
approved  labeling  to  add  new  safety  information,  imposition  of  post-market  studies  or  clinical  trials  to  assess  new  safety  risks  or  imposition  of
distribution or other restrictions under a Risk Evaluation and Mitigation Strategy program. Other potential consequences include, among other things: 

•
•
•

•
•

restrictions on the marketing or manufacturing of the product, complete withdrawal of the product from the market or product recalls;
fines, warning letters or holds on post-approval clinical trials;
refusal  of  the  FDA  to  approve  pending  applications  or  supplements  to  approved  applications,  or  suspension  or  revocation  of  product
approvals;
product seizure or detention, or refusal to permit the import or export of products; or
consent decrees, injunctions or the imposition of civil or criminal penalties.

The FDA strictly regulates marketing, labeling, advertising and promotion of products that are placed on the market. Drugs may be promoted only for
the  approved  indications  and  in  accordance  with  the  provisions  of  the  approved  label.  The  FDA  and  other  agencies  actively  enforce  the  laws  and
regulations  prohibiting  the  promotion  of  off  label  uses,  and  a  company  that  is  found  to  have  improperly  promoted  off  label  uses  may  be  subject  to
significant liability. 

Additional provisions 

In addition to FDA restrictions on marketing of pharmaceutical products, several other types of state and federal laws have been applied to restrict
certain  marketing  practices  in  the  pharmaceutical  industry  in  recent  years.  These  laws  include  anti-kickback  statutes  and  false  claims  statutes.  The 
federal anti-kickback statute prohibits, among other things, knowingly and willfully offering, paying, soliciting or receiving remuneration to induce or in
return  for  purchasing,  leasing,  ordering  or  arranging  for  the  purchase,  lease  or  order  of  any  healthcare  item  or  service  reimbursable  under  Medicare,
Medicaid  or  other  federally  financed  healthcare  programs.  This  statute  has  been  interpreted  to  apply  to  arrangements  between  pharmaceutical
manufacturers on the one hand and prescribers, purchasers and formulary managers on the other. Violations of the anti-kickback statute are punishable 
by imprisonment, criminal fines, civil monetary penalties and exclusion from participation in federal healthcare programs. Although there are a number
of statutory exemptions and regulatory safe harbors protecting certain common activities from prosecution or other regulatory sanctions, the exemptions
and  safe  harbors  are  drawn  narrowly,  and  practices  that  involve remuneration  intended  to  induce  prescribing,  purchases  or  recommendations  may  be
subject to scrutiny if they do not qualify for an exemption or safe harbor. 

Federal  false  claims  laws  prohibit  any  person  from  knowingly  presenting,  or  causing  to  be  presented,  a  false  claim  for  payment  to  the  federal
government,  or  knowingly  making,  or  causing  to  be  made,  a  false  statement  to  have  a  false  claim  paid.  Recently,  several  pharmaceutical  and  other
healthcare companies have been prosecuted under these laws for allegedly inflating drug prices they report to pricing services, which in turn were used
by the government to set Medicare and Medicaid reimbursement rates, and for allegedly providing free product to customers with the expectation that
the customers would bill federal programs for the product. In addition, certain marketing practices, including off-label promotion, may also violate false
claims laws. The majority of states also have statutes or regulations similar to the federal anti-kickback law and false claims laws, which apply to items 
and services reimbursed under Medicaid and other state programs, or, in several states, apply regardless of the payor. 

Physician Drug Samples 

As part of the sales and marketing process, pharmaceutical companies frequently provide samples of approved drugs to physicians. The Prescription
Drug Marketing Act, or the PDMA, imposes requirements and limitations upon the provision of drug samples to physicians, as well as prohibits states
from licensing distributors of prescription drugs unless the state licensing program meets certain federal guidelines that include minimum standards for
storage, handling and record keeping. In addition, the PDMA sets forth civil and criminal penalties for violations. 

Foreign Regulation 

In order to market any product outside of the U.S., we would need to comply with numerous and varying regulatory requirements of other countries
regarding  safety  and  efficacy  and  governing,  among  other  things,  clinical  trials,  marketing  authorization,  commercial  sales  and  distribution  of  our
products.  Whether  or  not  we  obtain  FDA  approval  for  a  product,  we  would  need  to  obtain  the  necessary  approvals  by  the  comparable  regulatory
authorities of foreign countries before we can commence clinical trials or marketing of the product in those countries. The approval process varies from
country to country and can involve additional product testing and additional administrative review periods. The time required to obtain approval in other
countries might differ from and be longer than that  required to obtain FDA approval. Regulatory  approval in one country does not ensure  regulatory
approval in another, but a failure or delay in obtaining regulatory approval in one country may negatively impact the regulatory process in others. 

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New Legislation and Regulations 

From time to time, legislation is drafted, introduced and passed in Congress that could significantly change the statutory provisions governing the
testing, approval, manufacturing and marketing of products regulated by the FDA. In addition to new legislation, FDA regulations and policies are often
revised  or  interpreted  by  the  agency  in  ways  that  may  significantly  affect  our  business  and  our  products.  It  is  impossible  to  predict  whether  further
legislative changes will be enacted or whether FDA regulations, guidance, policies or interpretations changed or what the effect of such changes, if any,
may be. 

Pharmaceutical Coverage, Pricing and Reimbursement 

Significant uncertainty exists as to the coverage and reimbursement status of any drug products for which we may obtain regulatory approval. Sales
of any of our product candidates, if approved, will depend, in part, on the extent to which the costs of the products will be covered by third-party payors, 
including government health programs such as Medicare and Medicaid,  commercial health insurers and managed care organizations. The  process for
determining whether a payor will provide coverage for a drug product may be separate from the process for setting the price or reimbursement rate that
the payor will pay for the drug product once coverage is approved. Third-party payors may limit coverage to specific drug products on an approved list,
or formulary, which might not include all of the approved drugs for a particular indication. 

In  order  to  secure  coverage  and  reimbursement  for  any  product  that  might  be  approved  for  sale,  we  may  need  to  conduct  expensive
pharmacoeconomic studies in order to demonstrate the medical necessity and cost-effectiveness of the product, in addition to the trials required to obtain
FDA or other comparable regulatory approvals. Our product candidates may not be considered medically necessary or cost-effective. A payor’s decision 
to provide coverage for a drug product does not imply that an adequate reimbursement rate will be approved. Third-party reimbursement may not be 
sufficient to enable us to maintain price levels high enough to realize an appropriate return on our investment in product development. 

The containment of healthcare costs has become a priority of federal, state and foreign governments, and the prices of drugs have been a focus in this
effort. Third-party payors are increasingly challenging the prices charged for medical products and services and examining the medical necessity and
cost-effectiveness of medical products and services, in addition to their safety and efficacy. If these third-party payors do not consider our products to be 
cost-effective compared to other available therapies, they may not cover our products after approval as a benefit under their plans or, if they do, the level
of  payment  may  not  be  sufficient  to  allow  us  to  sell  our  products  at  a  profit.  The  U.S.  government,  state  legislatures  and  foreign  governments  have
shown significant interest in implementing cost containment programs to limit the growth of government-paid health care costs, including price controls, 
restrictions  on  reimbursement  and  requirements  for  substitution  of  generic  products  for  branded  prescription  drugs.  Adoption  of  such  controls  and
measures, and tightening of restrictive policies in jurisdictions with existing controls and measures, could limit payments for pharmaceuticals such as the
drug candidates that we are developing and could adversely affect our net revenue and results. 

Pricing and reimbursement schemes vary widely from country to country. Some countries provide that drug products may be marketed only after a
reimbursement  price  has  been  agreed.  Some  countries  may  require  the  completion  of  additional  studies  that  compare  the  cost-effectiveness  of  a 
particular  product  candidate  to  currently  available  therapies.  For  example,  the  European  Union  provides  options  for  its  member  states  to  restrict  the
range  of  drug  products  for  which  their  national  health  insurance  systems  provide  reimbursement  and  to  control  the  prices  of  medicinal  products  for
human use. European Union member states may approve a specific price for a drug product or may instead adopt a system of direct or indirect controls
on the profitability of us placing the drug product on the market. Other member states allow companies to fix their own prices for drug products, but
monitor and control company profits. The downward pressure on health care costs in general, particularly prescription drugs, has become very intense.
As a result,  increasingly high  barriers are being  erected  to  the  entry  of new  products. In  addition,  in  some  countries,  cross-border imports  from low-
priced markets exert competitive pressure that may reduce pricing within a country. There can be no assurance that any country that has price controls or
reimbursement limitations for drug products will allow favorable reimbursement and pricing arrangements for any of our products. 

The marketability of any products for which we may receive regulatory approval for commercial sale may suffer if the government and third-party
payors  fail  to  provide  adequate  coverage  and  reimbursement.  In  addition,  an  increasing  emphasis  on managed  care  in  the  U.S.  has  increased  and  we
expect will continue to increase the pressure on drug pricing. Coverage policies, third-party reimbursement rates and drug pricing regulation may change 
at any time. In particular, the Patient Protection and Affordable Care Act was enacted in the U.S. in March 2010 and contains provisions that may reduce
the  profitability  of  drug  products,  including,  for  example,  increased  rebates  for  drugs  sold  to  Medicaid  programs,  extension  of  Medicaid  rebates  to
Medicaid managed care plans, mandatory discounts for certain Medicare Part D beneficiaries and annual fees based on pharmaceutical companies’ share 
of sales to federal health care programs. Even if favorable coverage and reimbursement status is attained for one or more products for which we receive
regulatory approval, less favorable coverage policies and reimbursement rates may be implemented in the future. 

Competition 

The  pharmaceutical  and  biotechnology  industry  is  very  competitive  and  the  development  and  commercialization  of  new  drugs  and  biologics  is
influenced by rapid technological developments and innovation. We face competition from several companies developing and commercializing products
that will be competitive with our drug candidates, including large pharmaceutical and smaller biotechnology companies.  Additionally, new entrants may
potentially  enter  the  market.  For  our  HBV  cure  program,  potential  competitors  include  Johnson  &  Johnson,  Roche,  Bristol  Myers  Squibb  Co.,
GlaxoSmithKline  PLC,  Gilead  Sciences  Inc.,  and  Arbutus  Biopharma  Corp.,  among  others.   Additionally,  we  may  face  competition  from  currently
available treatments for HBV. For CDI, our microbiome program’s first indication, our competitors include Seres Therapeutics, Inc. and Merck & Co,
Inc.  For our microbiome program more generally, our competitors include Johnson & Johnson, Novartis International AG, Abbvie Inc. and Pfizer Inc.
Some of the competitive development programs from these companies may be based on scientific approaches that are similar to our approach, and others
may  be  based  on  entirely  different  approaches.  Potential  competitors  also  include  academic  institutions,  government  agencies  and  other  public  and
private  research  organizations  that  conduct  research,  seek  patent  protection  and  establish  collaborative  arrangements  for  research,  development,
manufacturing and commercialization of products similar to ours or that otherwise target indications that we are pursuing. 

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Manufacturing 

We do not own or operate, and currently have no plans to establish, any manufacturing facilities. We currently rely, and expect to continue to rely, on
third parties for the manufacture of our product candidates for pre-clinical and clinical testing, as well as for commercial manufacture of any products
that we may commercialize. 

Financial Information 

We have not derived any revenue from product sales to date as it currently has no products.  

Research and Development Expense 

Our research and development expenses, excluding stock-based compensation expense, was $15,100,205 for fiscal year 2015, of which $10,810,517
was expended on the HBV Program and $4,296,309 was expended on our Microbiome Program, offset by $6,621 credit due to termination of VEN 307
study in 2014. 

Employees 

As  of  March  7,  2016,  we  had  51  employees,  and  various  consultants  and  multiple  research  contract  research  organizations  with  whom  we  have

contracted. 

Corporate History 

We were incorporated in Delaware in October 2005 under the name South Island Biosciences, Inc. (which was changed to Ventrus Biosciences, Inc.
in April 2007). On July 11, 2014, we merged with Assembly Pharmaceuticals, Inc., a private company (the “Merger”). In connection with the Merger, 
we changed our name from Ventrus Biosciences, Inc. to Assembly Biosciences, Inc. 

Corporate Information 

Our principal executive office is at 101 Sixth Avenue, Ninth Floor, New York, NY, 10013. Our telephone number is (646) 706-5208. 

Available Information 

Our website address is www.assemblybio.com. We routinely post, or have posted, important information for investors on our website in the “Investor
Relations” section. We use this website as a means of disclosing material information in compliance with our disclosure obligations under Regulation
FD.  Accordingly,  investors  should  monitor  the  “Investor  Relations”  section  of  our  website,  in  addition  to  following  our  press  releases,  SEC  filings,
presentations and webcasts. We make available free of charge through our website our press releases, Annual Report on Form 10-K, Quarterly Reports 
on Form 10-Q, Current Reports on Form 8-K and all amendments to those reports as soon as reasonably practicable after electronically filed with or
furnished to the Securities and Exchange Commission. 

The information contained on our website is not a part of, and should not be construed as being incorporated by reference, into this report. 

Information about Segments and Geographic Areas 

In  accordance  with The  Financial  Accounting  Standards  Board  (FASB)  Accounting  Standards  Codification,  or  ASC,  Topic  280,  Segment
Reporting, we have determined that we operate as one operating segment. Decisions regarding our overall operating performance and allocation of our
resources are assessed on a consolidated basis. Our operations and assets are predominantly located in the United States. 

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Item 1A. Risk Factors 

This report contains forward-looking statements that involve risks and uncertainties. Our actual results could differ materially from those discussed
in this report. Factors that could cause or contribute to these differences include, but are not limited to, those discussed below and elsewhere in this
report and in any documents incorporated in this report by reference. 

You should carefully consider the following risk factors, together with all other information in this report, including our financial statements and
notes thereto, and in our other filings with the Securities and Exchange Commission. If any of the following risks, or other risks not presently known to
us or that we currently believe to not be significant, develop into actual events, then our business, financial condition, results of operations or prospects
could be materially adversely affected. If that happens, the market price of our common stock could decline, and stockholders may lose all or part of
their investment. 

Risks Related to Our Business 

We have no approved products and currently are dependent on the success of our HBV and Microbiome programs. 

To  date,  we  have  no  approved  product  on  the market  and  have  generated  no  product  revenues.  Our  prospects  are  substantially  dependent  on  our
ability  to  develop  and  commercialize  our  HBV  and  microbiome  therapies.  Unless  and  until  we  receive  approval  from  the  FDA  and  other  regulatory
authorities  for  our  product  candidates,  we  cannot  sell  our  product  candidates  and  will  not  have  product  revenues.  We  will  have  to  fund  all  of  our
operations and capital expenditures from cash on hand, any licensing fees and any future securities offerings or debt financings and any fees we may
generate from out-licensing or other strategic arrangements. 

In addition, all of our product candidates are in an early stage of development and their risk of failure is high. The data supporting our drug discovery
and development programs are derived from either laboratory or pre-clinical studies. We cannot predict when or if any one of our product candidates
will prove effective or safe in humans or will receive regulatory approval. The scientific evidence to support the feasibility of our product candidates is
limited, and many companies, some with more resources than we have, are and may be developing competitive product candidates. For these and other
reasons, our drug discovery and development may not be successful and we may not generate viable products or revenue. 

We  depend  entirely  on  the  success  of  product  candidates  from  our  HBV  and  microbiome  programs,  both  of  which  are  in  late  pre-clinical 
development. We cannot be certain that we will be able to obtain regulatory approval for, or successfully commercialize, product candidates from
either of our current programs or any of our other product candidates we may subsequently identify. 

Our lead compounds for HBV and microbiome therapies are our only current product candidates. Both are in preclinical development. Neither of our
current  product  candidates  has  advanced  into  a  pivotal  study,  and  it  may  be  years  before  such  a  study  is  initiated,  if  ever.  The  clinical  trials  of  our
product candidates are, and the manufacturing and marketing of our product candidates will be, subject to extensive and rigorous review and regulation
by numerous government authorities in the U.S. and in other countries where we intend to test and, if approved, market any product candidate. Before
obtaining  regulatory  approvals  for  the  commercial  sale  of  any  product  candidate,  we  must  successfully  meet  a  number  of  critical  developmental
milestones, including: 

• developing dosages that will be tolerated, safe and effective;
• demonstrating through clinical trials that the product candidate is safe and effective in patients for the intended indication;
• determining the appropriate delivery mechanism;
• demonstrating that the product candidate formulation will be stable for commercially reasonable time periods; and
• completing the development and scale-up to permit manufacture of our product candidates in commercial quantities and at acceptable prices.

The  time  necessary  to  achieve  these  developmental  milestones  for  any  individual  product  candidate  is  long  and  uncertain,  and  we  may  not
successfully complete these milestones for our HBV and microbiome therapies or any other product candidates that we may develop. We have not yet
completed and may never complete the development of any product. If we are unable to complete development of our HBV or microbiome therapies, or
any other product candidates that we may develop, we will be unable to generate revenue or build a sustainable or profitable business. 

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We have a limited operating history and a history of operating losses, and expect to incur significant additional operating losses. 

We were established in October 2005, began active operations in the spring of 2007 and have only a limited operating history. In addition, we
have terminated our programs related to our three prior product candidates. Therefore, there is limited historical financial information upon  which to
base  an  evaluation  of  our  performance.  Our  prospects  must  be  considered  in  light  of  the  uncertainties,  risks,  expenses,  and  difficulties  frequently
encountered by companies in their early stages of operations. We, and Assembly Pharmaceuticals prior to our merger, have generated losses since we
began operations and, as of December 31, 2015, the combined company had an accumulated deficit of $164.0 million. We expect to incur substantial
additional  losses  over  the  next  several  years  as  we  continue  to  pursue  our  research,  development,  preclinical  studies  and  clinical  trial  activities.  The
amount of future losses and when, if ever, we will achieve profitability are uncertain. We have no products that have generated any commercial revenue,
do  not  expect  to  generate  revenues  from  the  commercial  sale  of  products  unless  and  until  our  HBV  or  microbiome  therapies  or  any  other  product
candidate is approved by the FDA for sale, and we might never generate revenues from the sale of products. 

We are not currently profitable and might never become profitable. 

We  have  a  history  of  losses  and  expect  to  incur  significant  operating  and  capital  expenditures  and  resultant  substantial  losses  and  negative
operating cash flow for the next several years, and beyond if we do not successfully launch and commercialize our HBV therapy or our microbiome
program. We might never achieve or maintain profitability. We anticipate that our expenses will continue to be substantial in the foreseeable future as
we: 

• continue to undertake research and development to identify potential product candidates;
• continue to undertake preclinical studies and clinical trials for our product candidates; and
• seek regulatory approvals for our product candidates.

As  a  result,  we  will  need  to  generate  significant  revenues  in  order  to  achieve  and  maintain  profitability.  Our  ability  to  generate  revenue  and

achieve profitability will depend on, among other things: 

• successful completion of research, preclinical studies and clinical trials for our product candidates;
• obtaining necessary regulatory approvals from the FDA and international regulatory agencies for our product candidates;
• establishing manufacturing, sales, and marketing arrangements with third parties for any approved products; and
• raising sufficient funds to finance our activities, if and when needed.

We  might  not  succeed  at  any  of  these  undertakings.  If  we  are  unsuccessful  at  some  or  all  of  these  undertakings,  our  business,  prospects,  and

results of operations might be materially adversely affected. 

Preclinical models may not be representative of disease behavior in clinical studies. Results of earlier clinical studies and trials may not be predictive
of future clinical trial results and preclinical testing and clinical trials involve a lengthy and expensive process with an uncertain outcome. 

The  results  of  preclinical  models  may  not  be  representative  of  disease  behavior  in  a  clinical  setting  and  thus  may  not  be  predictive  of  the
outcomes of our clinical trials. In addition, the results of preclinical studies and early clinical trials of product candidates may not be predictive of the
results of later-stage clinical trials and the results of any study or trial for any of our product candidates may not be as positive as the results for any prior
studies or trials, if at all. For example, in late June 2012, we reported that our second Phase III randomized, double-blind, placebo-controlled clinical trial 
of iferanserin in patients with hemorrhoidal disease did not meet its endpoints, despite favorable Phase II trial results. We also reported in February 2014
that  our  Phase  III  clinical  trial  for  diltiazem  for  the  treatment  of  anal  fissures  demonstrated  no  significant  improvement  compared  to  placebo  despite
favorable  results  in  a  prior  Phase  III  trial.  Based  on  these  unfavorable  clinical  results,  we  decided  to  cease  development  of  these  two  prior  product
candidates. These risks apply to our planned development of our current and any other product candidates. 

Preclinical studies and clinical testing are expensive, can take many years to complete and their outcome is highly uncertain. Failure can occur at
any time during the preclinical study and clinical trial processes due to inadequate performance of a drug candidate or inadequate adherence by patients
or investigators to clinical trial protocols. 

We are an early stage company and might not be able to commercialize any product candidates. 

We are an early stage company and have not demonstrated our ability to perform the functions necessary for the successful commercialization of

any product candidates. The successful commercialization of any product candidates will require us to perform a variety of functions, including: 

• continuing to undertake research and development and preclinical studies and clinical trials;
• participating in regulatory approval processes;
• formulating and manufacturing products; and

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• conducting sales, marketing and distribution activities.

Our development of our product candidates is subject to the risks of failure and delay inherent in the development of new pharmaceutical products

and products based on new technologies, including: 

• delays in product development, preclinical and clinical testing;
• unplanned expenditures in product development, preclinical and clinical testing;
• failure of a product candidate to demonstrate acceptable safety and efficacy;
• failure to receive regulatory approvals;
• emergence of superior or equivalent products;
• inability to manufacture and sell on our own, or through any others, product candidates on a commercial scale or at a financially viable cost; and
• failure to achieve market acceptance.

Because  of  these  risks,  our  research  and  development  efforts  might  not  result  in  any  commercially  viable  products.  If  we  do  not  successfully
complete  a  significant  portion  of  these  development  efforts,  obtain  required  regulatory  approvals,  and  have  commercial  success  with  any  approved
products, our business, financial condition and results of operations will be materially harmed. 

There are substantial risks inherent in attempting to commercialize new drugs, and, as a result, we may not be able to successfully develop products
for commercial use. 

Our  HBV  therapy  research  and  development  efforts  involve  therapeutics  based  on  modulating  forms  of  HBV  core  proteins  with  Core Protein
Allosteric Modulators, or CpAMs, which is a clinically unproven mechanism of action. The development of our CpAM technology is in the early stages,
and  the  commercial  feasibility  and  acceptance  of  our  CpAM  technology  are  unknown.  Similarly,  the  technology  for  our  microbiome  therapy  is  in
preclinical development. 

Scientific  research  and  development  requires  significant  amounts  of  capital  and  takes  a  long  time  to  reach  commercial  viability,  if  it  can  be
achieved at all. To date, our research and development projects have not produced commercially viable drugs, and may never do so. During the research
and development process, we may experience  technological barriers  that we may be unable to overcome. Further, certain underlying  premises in our
development programs are not fully proven.  More specifically, the theory that CpAMs can selectively reduce viral antigens in HBV patients and result
in a functional cure is unproven.  Thus, even if CpAM technology is successful at reducing antigen levels in HBV patients, it may not be a commercially
viable  drug  if  there  is  not  a  corresponding  medical  benefit  related  to  the  underlying  HBV  infection.  Similarly,  the  ability  to  effectively  and  reliably
deliver  bacteria  to  the  GI  tract  is  unproven,  and,  even  if  it  can  be  proven,  it  may  be  difficult  or  impossible  to  provide  the  treatment  economically.
Because  of  these  uncertainties,  it  is  possible  that  no  commercial  products  will  be  successfully  developed.  If  we  are  unable  to  successfully  develop
commercial products, we will be unable to generate revenue or build a sustainable or profitable business. 

We will need additional financing to complete the development of any product candidate and fund our activities in the future. 

We  anticipate  that  we  will  incur  operating  losses  for  the  next  several  years  as  we  continue  to  develop  our  HBV  therapy  and  our  microbiome
platform as well as initiate any development of any other product candidate and will require substantial funds during that time to support our operations.
We expect that our current resources will provide us with sufficient capital to fund our operations into the fourth quarter of 2017. However, we might
consume our available capital before that time if, for example, we are not efficient in managing our resources or if we encounter unforeseen costs, delays
or other issues or if regulatory requirements change. If that happens, we may need additional financing to continue the development of our HBV therapy
and our microbiome program. Thereafter, we will need additional capital to fund our operations in the future. However, there is no assurance that we will
be successful in raising any  necessary additional capital on terms that are acceptable to  us,  or at all.  If such event or other unforeseen circumstances
occurred and we were unable to raise capital, we could be forced to discontinue product development, sacrifice attractive business opportunities, cease
operations entirely and sell or otherwise transfer all or substantially all of our remaining assets. 

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Our product candidates face significant development and regulatory hurdles prior to marketing which could delay or prevent licensing, sales and/or
milestone revenue. 

Before we or any commercial partner obtains the approvals necessary to sell any of our product candidate, we must show through pre-clinical
studies and human testing in clinical trials that each potential product is safe and effective. The rates at which we complete our scientific studies and
clinical trials depend on many factors, including, but are not limited to, our ability to obtain adequate supplies of the products to be tested and patient
enrollment. Patient enrollment is a function of many factors, including the size of the patient population, the proximity of patients to clinical sites, the
eligibility criteria for the trial and other potential drug candidates being studied. Delays in patient enrollment for our trials may result in increased costs
and longer development times. In addition, we will need additional financing to develop our product candidates, which we might seek and receive from
third party commercial partners. Further, we currently do not have the infrastructure to market and sell our product candidates. If we partner with one or
more third party entities, those commercial partners may demand and receive rights to control product development and commercialization. As a result,
these commercial partners may conduct these programs and activities more slowly or in a different manner than expected. If any of these events were to
occur, the development of any product candidate could be significantly delayed, more expensive or less lucrative to us than anticipated, any of which
would have a significant adverse effect on our business. 

We are dependent on a license relationship for each of our HBV therapy and our microbiome program. 

Our  license  agreement  with  Indiana  University  Research  and  Technology  Corporation,  or  IURTC,  from  whom  we  have  licensed  our  HBV
therapy, requires us to make milestone payments based upon the successful accomplishment of clinical and regulatory milestones related to our HBV
therapy. The total amount of all potential future milestone payments at December 31, 2015 is $825,000. We also are obligated to pay IURTC royalty
payments based on net sales of the licensed technology. We are also obligated to pay diligence maintenance fees ($25,000-$100,000) each year to the 
extent  that  the  royalty,  sublicensing,  and  milestone  payments  to  IURTC  are  less  than  the  diligence  maintenance  fee  for  that  year.  Our  license  with
Therabiome, LLC, from whom we have licensed our microbiome program, also requires us to pay regulatory and clinical milestones as well as royalty
payments to Therabiome. If we breach any of these obligations, we could lose our rights to our microbiome program. If we fail to comply with similar
obligations to any other licensor, it would have the right to terminate the license, in which event we would not be able to commercialize drug candidates
or technologies that were covered by the license. Also, the milestone and other payments associated with licenses will make it less profitable for us to
develop our drug candidates than if we owned the technology ourselves. 

Our collaboration with Adam Zlotnick, the scientific founder of our HBV research program is advantageous. If that collaboration is not maintained,
we may not be able to capitalize on the market potential of our HBV cure program. 

Dr. Adam Zlotnick is the founder of our HBV research program. We have entered into a three-year consulting agreement with Dr. Zlotnick, the
initial term of which expires on July 11, 2017, pursuant to which he serves as the Chairman of our Scientific Advisory Board and provides consulting
services  as  we  request.  Dr.  Zlotnick  could  refuse  to  extend  the  agreement  after  its  expires  on  July  11,  2017  or  we  could  terminate  the  consulting
agreement for cause or no cause. Although Dr. Zlotnick assigned to us any rights to intellectual property related to our HBV therapy that arise during the
term  of  the  consulting  agreement,  and  while  the  consulting  agreement  contains  a  non-compete  during  the  term  of  the  agreement,  the  loss  of  Dr. 
Zlotnick’s services could materially impair our ability to further the development of our HBV therapy program. 

Corporate and academic collaborators might take actions to delay, prevent, or undermine the success of our product candidates. 

Our operating and financial strategy for the development, preclinical and clinical testing, manufacture, and commercialization of drug candidates
heavily depends on collaborating with corporations, academic institutions, licensors, licensees, and other parties. However, there can be no assurance
that  we  will  successfully  establish  these  collaborations.  In  addition,  should  a  collaboration  be  terminated,  replacement  collaborators  might  not  be
available on attractive terms, or at all. The activities of any collaborator will not be within our control and might not be within our power to influence.
There can be no assurance that any collaborator will perform its obligations to our satisfaction or at all, that we will derive any revenue or profits from
these  collaborations,  or  that  any  collaborator  will  not  compete  with  us.  If  any  collaboration  is  not  successful,  we  might  require  substantially  greater
capital to undertake development and marketing of our proposed products and might not be able to develop and market these products effectively, if at
all.  In  addition,  a  lack  of  development  and  marketing  collaborations  might  lead  to  significant  delays  in  introducing  proposed  products  into  certain
markets and/or reduced sales of proposed products in such markets. 

We  rely  on  data  provided  by  our  collaborators  and  others  that  has  not  been  independently  verified  and  could  prove  to  be  false,  misleading,  or
incomplete. 

We  rely  on  third-party  vendors,  scientists,  and  collaborators  to  provide  us  with  significant  data  and  other  information  related  to  our  projects,
preclinical studies and clinical trials, and our business. If these third parties provide inaccurate, misleading, or incomplete data, our business, prospects,
and results of operations could be materially adversely affected. 

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Preclinical and clinical testing required for our product candidates is expensive and time-consuming, and the outcome is uncertain. 

In  order  to  obtain  FDA  approval  to  market  a  new  drug  product,  we  must  demonstrate  safety  and  effectiveness  in  humans.  To  meet  these
requirements,  we  must  conduct  extensive  preclinical  testing  and  sufficient  adequate  and  well-controlled  clinical  trials.  Conducting  clinical  trials  is  a 
lengthy, time consuming, and expensive process. The length of time might vary substantially according to the type, complexity, novelty, and intended
use  of  the  product  candidate,  and  often  can  be  several  years  or  more  per  trial.  Delays  associated  with  product  candidates  for  which  we  are  directly
conducting  preclinical  studies  or  clinical  trials  might  cause  us  to  incur  additional  operating  expenses.  The  commencement  and  rate  of  completion  of
clinical trials might be delayed by many factors, including, for example: 

• the lack of effectiveness during clinical trials;
• the emergence of unforeseen safety issues;
• inability to manufacture sufficient quantities of qualified materials under current Good Manufacturing Practices, or cGMPs, for use in clinical trials;
• slower than expected rates of patient recruitment;
• failure to recruit a sufficient number of patients;
• modification of clinical trial protocols;
• changes in regulatory requirements for clinical trials;
• delays,  suspension,  or  termination  of  clinical  trials  by  the  institutional  review  board  or  ethics  committee  responsible  for  overseeing  the  study  at  a

particular study site; and

• government, institutional review board, ethics committee, or other regulatory delays or clinical holds requiring suspension or termination of the trials.  

The results from preclinical testing and early clinical trials are not necessarily predictive of results obtained in later clinical trials. Accordingly,
even  if  we  obtain  or  have  obtained  positive  results  from  preclinical  studies  or  early  clinical  trials,  we  might  not  achieve  the  same  success  in  future
clinical trials. For example, positive results were observed in earlier clinical trials of each of our two prior product candidates, but the subsequent clinical
trials were not successful. Further, clinical trials might not provide statistically significant data supporting a product candidate’s safety and effectiveness 
to meet the requisite regulatory approvals. 

We  have  used  and  intend  to  continue  to  rely  on  one  or  more  contract  research  organizations,  or  CROs,  to  conduct  our  preclinical  studies  and
clinical  trials.  We  are  highly  dependent  on  these  CROs  to  conduct  our  studies  and  trials  in  accordance  with  the  requirements  of  the  FDA  and  good
scientific practice. In the event the CROs fail to perform their duties in such a fashion, we may not obtain regulatory approval for any of our product
candidates. 

The failure of preclinical studies and clinical trials to demonstrate safety and effectiveness for the desired indications could harm the development
of that product candidate and other product candidates. This failure could cause us to abandon a product candidate and could delay development of other
product candidates. Any delay in, or termination of, our preclinical studies or clinical trials would delay the filing of our New Drug Applications, or
NDAs, with the FDA and, ultimately, our ability to commercialize our product candidates and generate product revenues. Any change in, or termination
of, our clinical trials could materially harm our business, financial condition, and results of operation. 

Research, development and commercialization goals may not be achieved in the time frames that we publicly estimate, which could have an adverse
impact on our business and could cause our stock price to decline. 

We  set  goals,  and  make  public  statements  regarding  our  expectations,  regarding  the  timing  of  certain  accomplishments,  developments  and
milestones under our research and development programs. The actual timing of these events can vary significantly due to a number of factors, including,
without limitation, the amount of time, effort and resources committed to our programs by us and any collaborators and the uncertainties inherent in the
regulatory approval process. As a result, there can be no assurance that we or any collaborators will make regulatory submissions or receive regulatory
approvals as planned or that we or any collaborators will be able to adhere to our current schedule for the achievement of key milestones under any of
our programs. If we or any collaborators fail to achieve one or more of the milestones as planned, our business could be materially adversely affected
and the price of our common stock could decline. 

Unforeseen safety issues could hinder the development of our product candidates and their adoption, if approved. 

Safety  issues  could  arise  during  development  of  our  product  candidates,  which  might  delay  testing  or  prevent  further  development  entirely.
Unforeseen  safety  issues  could  emerge  in  any  future  study  or  trial  of  our  HBV  or  microbiome  product  candidates,  which  could  severely  hamper  the
likelihood of FDA or other regulatory approval of any such product candidate. If any of these events were to occur, the development of any product
candidate could be significantly delayed and become more expensive than anticipated, and could lead us to abandon our development efforts entirely,
any of which would have a significant adverse effect on our business. 

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If  a  product  is  approved,  any  limitation  on  use  that  might  be  necessary  due  to  safety  issues  could  hinder  its  adoption  in  the  marketplace.  In

addition, if any product is approved, it could be used against any instructions that we publish that limit its use, which could subject us to litigation. 

We  lack  suitable  facilities  for  certain  preclinical  and  clinical  testing  and  expect  to  rely  on  third  parties  to  conduct  some  of  our  research  and
preclinical testing and our clinical trials and those third parties may not perform satisfactorily, including failing to meet deadlines for the completion
of such research, testing or trials. 

We do not have sufficient facilities to conduct all of our anticipated preclinical and clinical testing. As a result, we expect to contract with third
parties to conduct most or all preclinical and clinical testing required for regulatory approval for our product candidates. We currently plan to outsource
all clinical testing to third parties and will be reliant on the services of these third parties to conduct studies on our behalf. If we are unable to retain or
continue with third parties for these purposes on acceptable terms, we may be unable to successfully develop our product candidates. In addition, any
failures by third parties to adequately perform their responsibilities may delay the submission of our product candidates for regulatory approval, which
would impair our financial condition and business prospects. 

Our reliance on these third parties for research and development activities also reduces our control over these activities but will not relieve us of
our responsibilities. For example, we are responsible for ensuring that each of our studies is conducted in accordance with the applicable protocol, legal
and regulatory requirements and scientific standards, and our reliance on third parties does not relieve us of our regulatory responsibilities. Furthermore,
these third  parties may also have relationships with other entities, some of which may be our competitors. In addition, these third parties are not our
employees, and except for remedies available to us under our agreements with such third parties, we cannot control whether or not they devote sufficient
time and resources to our clinical and preclinical programs. If these third parties do not successfully carry out their contractual duties or obligations or
meet expected deadlines, if they need to be replaced or if the quality or accuracy of the clinical data they obtain is compromised due to the failure to
adhere to our clinical protocols, regulatory requirements or for other reasons, our research, preclinical studies or clinical trials may be extended, delayed
or terminated and we may not be able to obtain, or may be delayed in obtaining, regulatory approvals for our product candidates. As a result, our results
of operations and business prospects would be harmed, our costs could increase and our ability to generate revenues could be delayed. 

We will rely exclusively on third parties to formulate and manufacture our product candidates. 

We  do  not  have  and  do  not  intend  to  establish  our  own  manufacturing  facilities.  Consequently,  we  lack  the  physical  plant  to  formulate  and
manufacture our own product candidates for use in our planned clinical trials. In addition, if any product candidate we might develop or acquire in the
future receives FDA approval, we will rely on one or more third-party contractors to manufacture our products. If, for any reason, we become unable to
rely  on  any  future  source  to  manufacture  our  product  candidates,  either  for  clinical  trials  or,  at  some  future  date,  for  commercial  quantities,  then  we
would  need  to  identify  and  contract  with  additional  or  replacement  third-party  manufacturers  to  manufacture  compounds  for  preclinical,  clinical  and
commercial purposes. We might not be successful in identifying additional or replacement third-party manufacturers, or in negotiating acceptable terms 
with any that we do identify. If we are unable to secure and maintain third-party manufacturing capacity, the development and sales of our products and
our financial performance might be materially affected. 

In  addition,  before  any  of  our  collaborators  can  begin  to  commercially  manufacture  our  product  candidates,  each  manufacturing  facility  and
process  is  subject  to  regulatory  review.  Manufacturing  of  drugs  for  clinical  and  commercial  purposes  must  comply  with  the  FDA’s  cGMPs,  and 
applicable  non-U.S.  regulatory requirements. The  cGMP  requirements govern quality  control and  documentation policies and procedures. Complying
with cGMP and non-U.S. regulatory requirements will require that we expend time, money, and effort in production, recordkeeping, and quality control
to assure that the product meets applicable specifications and other requirements. Any contracted manufacturing facility must also pass a pre-approval 
inspection prior to FDA approval. Failure to pass a pre-approval inspection might significantly delay FDA approval of our product candidates. If any of
our future collaborators fails to comply with these requirements, it would be subject to possible regulatory action which could limit the jurisdictions in
which we are  permitted to sell  our products,  if  approved.  As  a  result, our business,  financial  condition, and results of  operations  might be materially
harmed. 

Our reliance on third-party manufacturers exposes us to the following risks: 

•

•

•

•

We  might  be  unable  to  identify  manufacturers  for  commercial  supply  on  acceptable  terms  or  at  all  because  the  number  of  potential
manufacturers  is  limited  and  the  FDA  must  approve  any  replacement  contractor.  This  approval  would  generally  require  compliance
inspections. In addition, a new manufacturer would have to be educated in, or develop substantially equivalent processes for, production of
our products after receipt of FDA approval, if any.
Our  third-party  manufacturers  might  be  unable  to  formulate  and  manufacture  our  product  candidates  in  the  volume  and  of  the  quality
required to meet our clinical and, if approved, commercial needs.
Our contract manufacturers might not perform as agreed or might not remain in the contract manufacturing business for the time required
to supply our clinical trials or to successfully produce, store and distribute our products.
One  or  more  of  our  contract  manufacturers  could  be  foreign,  which  increases  the  risk  of  shipping  delays  and  adds  the  risk  of  import
restrictions.

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•

•

•

Drug manufacturers are subject to ongoing periodic unannounced inspection by the FDA and corresponding state agencies to ensure strict
compliance with cGMP and other government regulations and corresponding foreign requirements. We would not have complete control
over third-party manufacturers’ compliance with these regulations and requirements.
If any third-party manufacturer makes improvements in the manufacturing process for our product candidates, we might not own, or might
have to share, the intellectual property rights to the innovation with our licensors.
We might compete with other companies for access to these manufacturers’ facilities and might be subject to manufacturing delays if the
manufacturers give other clients higher priority than us.

Each of these risks could delay our development efforts, preclinical studies and clinical trials or the approval, if any, of our product candidates by
the FDA or the commercialization of our product candidates and could result in higher costs or deprive us of potential product revenues. As a result, our
business, financial condition, and results of operations might be materially harmed. 

If  we  cannot  compete  successfully  for  market  share  against  other  drug  companies,  we  might  not  achieve  sufficient  product  revenues  and  our
business will suffer. 

If  our  product  candidates  receive  FDA  approval,  they  will  compete  with  a  number  of  existing  and  future  drugs  and  therapies  developed,
manufactured and marketed by others. Existing or future competing drugs might provide greater therapeutic convenience or clinical or other benefits for
a specific indication than our product candidates, or might offer comparable performance at a lower cost. If our product candidates fail to capture and
maintain market share, we might not achieve sufficient product revenues and our business will suffer. 

We might compete against fully integrated pharmaceutical companies and smaller companies that are collaborating with larger pharmaceutical
companies, academic institutions, government agencies and other public and private research organizations. Many of these competitors, either alone or
together with their collaborative partners, operate larger research and development programs or have substantially greater financial resources than we do,
as well as significantly greater experience in: 

•
•
•
•
•

developing drugs;
undertaking preclinical testing and human clinical trials;
obtaining FDA and other regulatory approvals of drugs;
formulating and manufacturing drugs; and
launching, marketing and selling drugs.

We may not have or be able to obtain the same resources and experience as our competitors. If we are unable to perform these tasks effectively

and efficiently, our results of operations might be materially adversely affected. 

Developments by competitors might render our product candidates or technologies obsolete or non-competitive. 

The pharmaceutical and biotechnology industries are intensely competitive. In addition, the clinical and commercial landscape for HBV and CDI
is rapidly changing; we expect new data from commercial and clinical-stage products to continue to emerge. We will compete with organizations that
have existing treatments and that are or will be developing treatments for the indications that our product candidates target. If our competitors develop
effective treatments for HBV, CDI or any other indication or field we might pursue, and successfully commercialize those treatments, our business and
prospects might be materially harmed, due to intense competition in these markets. 

If  we  are  not  able  to  develop  collaborative  marketing  relationships  with  licensees  or  partners,  or  create  effective  internal  sales,  marketing,  and
distribution capability, we might be unable to market our products successfully. 

To market our product candidates, if approved, we will have to establish our own marketing and sales force or out-license our product candidates
to, or collaborate with, larger firms with experience in marketing and selling pharmaceutical products. There can be no assurance that we will be able to
successfully establish our own marketing capabilities or establish marketing, sales, or distribution relationships with third parties; that such relationships,
if established, will be successful; or that we will be successful in gaining market acceptance for our product candidates. To the extent that we enter into
any  marketing,  sales,  or  distribution  arrangements  with  third  parties,  our  product  revenues  will  be  lower  than  if  we  marketed  and  sold  our  products
directly, and any revenues we receive will depend upon the efforts of such third parties. If we are unable to establish such third-party sales and marketing 
relationships, or choose not to do so, we will have to establish our own in-house capabilities. We, as a company, have no experience in marketing or
selling pharmaceutical products and currently have no sales, marketing, or distribution infrastructure. To market any of our products directly, we would
need to develop a marketing, sales, and distribution force that both has technical expertise and the ability to support a distribution capability. To establish
our own marketing, sales, and distribution capacity would significantly increase our costs, and require substantial additional capital. In addition, there is
intense competition for proficient sales and marketing personnel, and we might not be able to attract individuals who have the qualifications necessary to
market, sell, and distribute our products. There can be no assurance that we will be able to establish internal marketing, sales, or distribution capabilities. 

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The commercial success of our product candidates will depend upon the degree of market acceptance by physicians, patients, third-party payers and 
others in the medical community. 

The commercial success of our products, if approved for marketing, will depend in part on the medical community, patients and third-party payers
accepting our product candidates as effective and safe. If these products do not achieve an adequate level of acceptance, we may not generate significant
product revenue and may not become profitable. The degree of market acceptance of our products, if approved for marketing, will depend on a number
of factors, including: 

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the actual or perceived safety and efficacy of the products, and advantages over alternative treatments; 
the pricing and cost-effectiveness of our products relative to competing products or therapies;
the labeling of any approved product;
the prevalence and severity of any side effects, including any limitations or warnings contained in a product's approved labeling; 
the emergence, and timing of market introduction, of competitive products; 
the effectiveness of marketing and distribution efforts by us and our licensees and distributors, if any; and 
the availability of third-party insurance coverage or governmental reimbursement.

Even  if  a  potential  product  displays  a  favorable  efficacy  and  safety  profile  in  preclinical  studies  and  clinical  trials,  market  acceptance  of  the
product will not be known until after it is launched. Any failure to achieve market acceptance for our product candidates will harm our business, results
and financial condition. 

If  we  lose  key  management  or  scientific  personnel,  cannot  recruit  qualified  employees,  directors,  officers,  or  other  significant  personnel  or
experience increases in our compensation costs, our business might materially suffer. 

We  are  highly  dependent  on  the  services  of  our  Chief  Executive  Officer  and  President,  Derek  Small,  our  Chief  Medical  Officer  and  Vice
President  of  Research  and  Development,  Dr.  Uri  Lopatin,  our  Chief  Scientific  Officer,  Dr.  Richard  Colonno,  our  Chief  Discovery  Officer,  Lee  D.
Arnold,  our  Chief  Development  Officer  and  Head  of  Microbiome,  Thomas  E.  Rollins,  and  our  Chief  Financial  Officer  and  Chief  Operating  Officer,
David J. Barrett. Our employment agreements with Mr. Small, Dr. Lopatin, Dr. Colonno, Dr. Arnold, Mr. Rollins and Mr. Barrett do not ensure their
retention. This is also true for our other management team members, both present and future. 

Furthermore,  our  future  success  also  depends,  in  part,  on  our  ability  to  identify,  hire,  and  retain  additional  management  team  members  as  our
operations grow. We expect to experience intense competition for qualified personnel and might be unable to attract and retain the personnel necessary
for the development of our business. Finally, we do not currently maintain, nor do we intend to obtain in the future, “key man” life insurance that would 
compensate us in the event of the death or disability of any of the members of our management team. 

The  failure  by  us  to  retain,  attract  and  motivate  executives  and  other  key  employees  could  have  a  material  adverse  impact  on  our  business,

financial condition and results of operations. 

If we are unable to hire additional qualified personnel, our ability to grow our business might be harmed. 

As of March 7, 2016, we had 51 employees, and various consultants and multiple contract research organizations with whom we have contracted.
We will need to hire or contract with additional qualified personnel with expertise in clinical research and testing, formulation and manufacturing and
sales  and  marketing  to  commercialize  our  HBV  therapy  and  our  microbiome  program  or  any  other  product  candidate  we  may  seek  to  develop.  We
compete  for  qualified  individuals  with  numerous  biopharmaceutical  companies,  universities  and  other  research  institutions.  Competition  for  these
individuals is intense, and we cannot be certain that our search for such personnel will be successful. Attracting and retaining qualified personnel will be
critical to our success. 

We might not successfully manage our growth. 

Our success will depend upon the expansion of our operations and the effective management of our growth, which will place a significant strain
on our current and future management and other administrative and operational resources. To manage this growth, we may need to expand our facilities,
augment our operational, financial and management systems and hire and train additional qualified personnel. If we are unable to manage our growth
effectively, our business would be harmed. 

We might seek to develop our business through acquisitions of or investment in new or complementary businesses, products or technologies, and the
failure to manage these acquisitions or investments, or the failure to integrate them with our existing business, could have a material adverse effect
on us. 

We  might  consider  opportunities  to  acquire  or  invest  in  other  technologies,  products  and  businesses  that  might  enhance  our  capabilities  or
complement our current product candidates. Potential and completed acquisitions and strategic investments involve numerous risks, including potential
problems or issues associated with the following: 

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assimilating the purchased technologies, products or business operations;
maintaining uniform standards, procedures, controls and policies;
unanticipated costs associated with the acquisition or investment;
diversion of our management’s attention from our preexisting business;
maintaining or obtaining the necessary regulatory approvals or complying with regulatory requirements; and
adverse effects on existing business operations.

We have no current commitments with respect to any acquisition or investment in other technologies or businesses. We do not know if we will
identify suitable acquisitions, whether we will be able to successfully complete any acquisitions, or whether we will be able to successfully integrate any
acquired product, technology or business into our business or retain key personnel, suppliers or collaborators. 

Our ability to successfully develop our business through acquisitions would depend on our ability to identify, negotiate, complete and integrate
suitable target businesses or technologies and obtain any necessary financing. These efforts could be expensive and time consuming and might disrupt
our  ongoing  operations.  If  we  are  unable  to  efficiently  integrate  any  acquired  business,  technology  or  product  into  our  business,  our  business  and
financial condition might be adversely affected. 

Risks Related to Our Regulatory and Legal Environment 

We are subject to extensive and costly government regulation. 

Product candidates employing our technology are subject to extensive and rigorous domestic government regulation including regulation by the
FDA, the Centers for Medicare and Medicaid Services, other divisions of the U.S. Department of Health and Human Services, the U.S. Department of
Justice,  state  and  local  governments,  and  their  respective  foreign  equivalents.  The  FDA  regulates  the  research,  development,  preclinical  and  clinical
testing, manufacture, safety, effectiveness, record-keeping, reporting, labeling, storage, approval, advertising, promotion, sale, distribution, import, and
export of pharmaceutical products. The FDA regulates small molecule chemical entities, whether administered orally, topically or by injection, as drugs,
subject to an NDA, under the Federal Food, Drug, and Cosmetic Act. If products employing our technologies are marketed abroad, they will also be
subject to extensive regulation by foreign governments, whether or not they have obtained FDA approval for a given product and its uses. Such foreign
regulation might be equally or more demanding than corresponding U.S. regulation. 

Government  regulation  substantially  increases  the  cost  and  risk  of  researching,  developing,  manufacturing,  and  selling  our  product  candidates.
The regulatory review and approval process, which includes preclinical testing and clinical trials of each product candidate, is lengthy, expensive, and
uncertain. We or our collaborators must obtain and maintain regulatory authorization to conduct clinical trials and approval for each product we intend to
market,  and  the  manufacturing  facilities  used  for  the  products  must  be  inspected  and  meet  legal  requirements.  Securing  regulatory  approval  requires
submitting  extensive  preclinical  and  clinical  data  and  other  supporting  information  for  each  proposed  therapeutic  indication  in  order  to  establish  the
product’s safety and efficacy for each intended use. The development and approval process might take many years, requires substantial resources, and
might never lead to the approval of a product. 

Even if we are able to obtain regulatory approval for a particular product, the approval might limit the intended medical uses for the product, limit
our  ability  to  promote,  sell,  and  distribute  the  product,  require  that  we  conduct  costly  post-marketing  surveillance,  and/or  require  that  we  conduct 
ongoing  post-marketing  studies.  Material  changes  to  an  approved  product,  such  as,  for  example,  manufacturing  changes  or  revised  labeling,  might
require further regulatory review and approval. Once obtained, any approvals might be withdrawn, including, for example, if there is a later discovery of
previously unknown problems with the product, such as a previously unknown safety issue. 

If we, our collaborators, or our contract manufacturers fail to comply with applicable regulatory requirements at any stage during the regulatory
process, such noncompliance could result in, among other things, delays in the approval of applications or supplements to approved applications; refusal
by a regulatory authority, including the FDA, to review pending market approval applications or supplements to approved applications; untitled letters or
warning letters; fines; import and export restrictions; product recalls or seizures; injunctions; total or partial suspension of production; civil penalties;
withdrawals  of  previously  approved  marketing  applications;  recommendations  by  the  FDA  or  other  regulatory  authorities  against  governmental
contracts; and/or criminal prosecutions. 

We might not obtain the necessary U.S. or worldwide regulatory approvals to commercialize any product candidate. 

We  cannot  assure  you  that  we  will  receive  the  approvals  necessary  to  commercialize  for  sale  any  of  our  product  candidates,  or  any  product
candidate we acquire or develop in the future. We will need FDA approval to commercialize our product candidates in the U.S. and approvals from the
FDA-equivalent  regulatory  authorities  in  foreign  jurisdictions  to  commercialize  our  product  candidates  in  those  jurisdictions. In  order  to  obtain  FDA
approval of any product candidate, we must submit to the FDA an NDA demonstrating that the product candidate is safe for humans and effective for its
intended use. This demonstration requires significant research, preclinical studies, and clinical trials. Satisfaction of the FDA’s regulatory requirements 
typically  takes  many  years,  depends  upon  the  type,  complexity  and  novelty  of  the  product  candidate  and  requires  substantial  resources  for  research,
development and testing. We cannot predict whether our research and clinical approaches will result in drugs that the FDA considers safe for humans
and  effective  for  their  indicated  uses.  The  FDA  has  substantial  discretion  in  the  drug  approval  process  and  might  require  us  to  conduct  additional
preclinical and clinical testing, perform post-marketing studies or otherwise limit or impose conditions on any approval we obtain. 

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The approval process might also be delayed by changes in government regulation, future legislation or administrative action or changes in FDA

policy that occur prior to or during our regulatory review. Delays in obtaining regulatory approvals might: 

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delay commercialization of, and our ability to derive product revenues from, our product candidates;
impose costly procedures on us; and
diminish any competitive advantages that we might otherwise enjoy.

Even if we comply with all FDA requests, the FDA might ultimately reject one or more of our NDAs. We cannot be sure that we will ever obtain
regulatory  approval  for  our  product  candidates.  Failure  to  obtain  FDA  approval  of  our  product  candidates  will  severely  undermine  our  business  by
leaving us without a saleable product, and therefore without any source of revenues, until another product candidate could be developed or obtained.
There is no guarantee that we will ever be able to develop an existing, or acquire another, product candidate. 

In  foreign  jurisdictions,  we  must  receive  approval  from  the  appropriate  regulatory  authorities  before  we  can  commercialize  any  product
candidates.  The  risks  associated  with  foreign  regulatory  approval  processes  are  similar  to  the  risks  associated  with  the  FDA  approval  procedures
described above. We cannot assure you that we will receive the approvals necessary to commercialize our product candidates for sale outside the U.S. 

Even if approved, our product candidates will be subject to extensive post-approval regulation. 

Once  a  product  candidate  is  approved,  numerous  post-approval  requirements  apply.  Among  other  things,  the  holder  of  an  approved  NDA  is
subject to ongoing FDA oversight monitoring and reporting obligations, including obligations to monitor and report adverse events and instances of the
failure of a product to meet the specifications in the NDA. Application holders must submit new or supplemental applications and obtain FDA approval
for changes to the approved product, product labeling, or manufacturing process, depending on the nature of the change. Application holders also must
submit advertising and other promotional material to the FDA and report on ongoing clinical trials. The FDA also has the authority to require changes in
the labeling of approved drug products and to require post-marketing studies. 

Advertising  and  promotional  materials  must  comply  with  FDA  rules  in  addition  to  other  applicable  federal  and  state  laws.  The  distribution  of
product samples to physicians must comply with the requirements of the Prescription Drug Marketing Act. Manufacturing facilities remain subject to
FDA  inspection  and  must  continue  to  adhere  to  the  FDA’s  cGMP  requirements.  Sales,  marketing,  and  scientific/educational  grant  programs,  among
other activities, must comply with the anti-fraud and abuse provisions of the Social Security Act, the False Claims Act, and similar state laws, each as
amended. Pricing and rebate programs must comply with the Medicaid rebate requirements of the Omnibus Budget Reconciliation Act of 1990 and the
Veteran’s Health Care Act of 1992, each as amended. If products are made available to authorized users of the Federal Supply Schedule of the General
Services  Administration,  additional  laws  and  requirements  apply.  All  of  these  activities  are  also  potentially  subject  to  federal  and  state  consumer
protection and unfair competition laws. 

Depending on the circumstances, failure to meet these post-approval requirements can result in criminal prosecution, fines, injunctions, recall or
seizure of products, total or partial suspension of production, denial or withdrawal of pre-marketing product approvals, or refusal to allow us to enter into 
supply  contracts,  including  government  contracts.  In  addition,  even  if  we  comply  with  FDA  and  other  requirements,  new  information  regarding  the
safety or effectiveness of a product could lead the FDA to modify or withdraw product approval. 

Even  if  we  are  able  to  commercialize  any  product  candidates,  those  products  may  become  subject  to  unfavorable  pricing  regulations,  third  party
reimbursement practices or healthcare reform initiatives, which would harm our business. 

The regulations that govern marketing approvals, pricing and reimbursement for new medicines vary widely from country to country. In the U.S.,
recently enacted legislation may significantly change the approval requirements in ways that could involve additional costs and cause delays in obtaining
approvals. Some countries require approval of the sale price of a medicine before it can be marketed. In many countries, the pricing review period begins
after  marketing  or  product  licensing  approval  is  granted.  In  some  foreign  markets,  prescription  pharmaceutical  pricing  remains  subject  to  continuing
governmental control even after initial approval is granted. As a result, we might obtain marketing approval for a medicine in a particular country, but
then  be  subject  to  price  regulations  that  delay  our  commercial  launch  of  the  medicine,  possibly  for  lengthy  time  periods,  and  negatively  impact  the
revenues  we  are  able  to  generate  from  the  sale  of  the  medicine  in  that  country.  Adverse  pricing  limitations  may  hinder  our  ability  to  recoup  our
investment in one or more product candidates, even if our product candidates obtain marketing approval. 

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Our ability to commercialize any medicines successfully also will depend in part on the extent to which reimbursement for these medicines and
related  treatments  will  be  available  from  government  health  administration  authorities,  private  health  insurers  and  other  organizations.  Government
authorities and third party payors, such as private health insurers and health maintenance organizations, decide which medications they will pay for and
establish  reimbursement  levels.  A  primary  trend  in  the  U.S.  healthcare  industry and  elsewhere  is  cost  containment.  Government  authorities  and  third
party payors have attempted to control costs by limiting coverage and the amount of reimbursement for particular medications. Increasingly, third party
payors are requiring that drug companies provide them with predetermined discounts from list prices and are challenging the prices charged for medical
products. We cannot be sure that reimbursement will be available for any product candidate that we commercialize and, if reimbursement is available,
the level of reimbursement. Reimbursement may impact the demand for, or the price of, any product candidate for which we obtain marketing approval.
If  reimbursement  is  not  available  or  is  available  only  to  limited  levels,  we  may  not  be  able  to  successfully  commercialize  any product  candidate  for
which we obtain marketing approval. 

There may be significant delays in obtaining reimbursement for newly approved medicines, and coverage may be more limited than the purposes
for which the medicine is approved by the FDA or similar regulatory authorities outside the U.S. Moreover, eligibility for reimbursement does not imply
that  any  medicine will be  paid  for in  all  cases  or  at  a  rate that  covers  our  costs,  including research,  development,  manufacture,  sale  and  distribution.
Interim  reimbursement  levels  for  new  medicines,  if  applicable,  may  also  not  be  sufficient  to  cover  our  costs  and  may  not  be  made  permanent.
Reimbursement rates may vary according to the use of the medicine and the clinical setting in which it is used, may be based on reimbursement levels
already set for lower cost medicines and may be incorporated into existing payments for other services. Net prices for medicines may be reduced by
mandatory discounts or rebates required by government healthcare programs or private payors and by any future relaxation of laws that presently restrict
imports  of  medicines  from  countries  where  they  may  be  sold  at  lower  prices  than  in  the  U.S.  Third  party  payors  often  rely  upon  Medicare  coverage
policy and payment limitations in setting their own reimbursement policies. Our inability to promptly obtain coverage and profitable payment rates from
both government-funded and private payors for any approved product candidates that we develop could have a material adverse effect on our operating
results, our ability to raise capital needed to commercialize product candidates and our overall financial condition. 

In the U.S. and in other countries, there have been and we expect there will continue to be a number of legislative and regulatory proposals to
change the healthcare system in ways that could significantly affect our business. International, federal and state lawmakers regularly propose and, at
times, enact legislation that would result in significant changes to the healthcare system, some of which are intended to contain or reduce the costs of
medical products and services. The U.S. government and other governments have shown significant interest in pursuing healthcare reform, as evidenced
by  the  Patient  Protection  and  Affordable  Care  Act  and  its  amendment,  the  Health  Care  and  Education  Reconciliation  Act.  Such  government-adopted 
reform measures may adversely impact the pricing of healthcare products and services in the U.S. or internationally and the amount of reimbursement
available from governmental agencies or other third-party payors. In addition, in some foreign jurisdictions, there have been a number of legislative and
regulatory proposals to change the healthcare system in ways that could affect our ability to sell our products profitably. The continuing efforts of U.S.
and other governments, insurance companies, managed care organizations and other payors of healthcare services to contain or reduce healthcare costs
may adversely affect our ability to set satisfactory prices for our products, to generate revenues, and to achieve and maintain profitability. 

We face the risk of product liability claims and might not be able to obtain insurance. 

Our business exposes us to the risk of product liability claims that are inherent in the development of drugs. If the use of one or more of our or our
collaborators’ product candidates or approved drugs, if any, harms people, we might be subject to costly and damaging product liability claims brought
against us by clinical trial participants, consumers, health care providers, pharmaceutical companies or others selling our products. Our inability to obtain
sufficient  product  liability  insurance  at  an  acceptable  cost  to  protect  against  potential  product  liability  claims  could  prevent  or  inhibit  the
commercialization  of  pharmaceutical  products  we  develop.  We  expect  to  obtain  clinical  trial  insurance  for  our  product  candidates  prior  to  beginning
clinical trials. We cannot predict all of the possible harms or side effects that might result and, therefore, the amount of insurance coverage we obtain, if
any, in the future might not be adequate to cover all liabilities we might incur. We intend to expand our insurance coverage to include product liability
insurance covering the sale of commercial products if we obtain marketing approval for our drug candidates in development, but we might be unable to
obtain  commercially  reasonable  product  liability  insurance  for  any  products  approved  for  marketing.  If  we  are  unable  to  obtain  insurance  at  an
acceptable cost or otherwise protect against potential product liability claims, we will be exposed to significant liabilities, which might materially and
adversely affect our business and financial position. If we are sued for any injury allegedly caused by our or our collaborators’ products, our liability 
could exceed our total assets and our ability to pay the liability. Any successful product liability claims or series of claims brought against us would
decrease our cash and could cause the value of our common stock to decrease. 

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We might be exposed to liability claims associated with the use of hazardous materials and chemicals. 

Our research, development and manufacturing activities and/or those of our third-party contractors might involve the controlled use of hazardous
materials and chemicals. Although we will strive to have our safety procedures, and those of our contractors, for using, storing, handling and disposing
of  these  materials  comply  with  federal,  state  and  local  laws  and  regulations,  we  cannot  completely  eliminate  the  risk  of  accidental  injury  or
contamination  from  these  materials.  In  the  event  of  such  an  accident,  we  could  be  held  liable  for  any  resulting  damages,  and  any  liability  could
materially  adversely  affect  our  business,  financial  condition  and  results  of  operations.  In  addition,  the  federal,  state  and  local  laws  and  regulations
governing  the  use,  manufacture,  storage,  handling  and  disposal  of  hazardous  or  radioactive  materials  and  waste  products  might  require  us  to  incur
substantial compliance costs that could materially adversely affect our business, financial condition and results of operations. We currently do not carry
hazardous materials liability insurance. We intend to obtain such insurance in the future if necessary, but cannot give assurance that we could obtain
such coverage. 

Risks Related to Our Intellectual Property 

Our business depends on protecting our intellectual property. 

If we and our licensors IURTC and Therabiome do not obtain protection for our respective intellectual property rights, our competitors might be
able to take advantage of our research and development efforts to develop competing drugs. Our success, competitive position and future revenues, if
any, depend in part on our ability and the abilities of our licensors to obtain and maintain patent protection for our products, methods, processes and
other technologies, to preserve our trade secrets, to prevent third parties from infringing on our proprietary rights and to operate without infringing the
proprietary rights of third parties. 

We seek to protect our proprietary position by filing patent applications in the U.S. and abroad related to our novel technologies and chemical and
biological compositions that are important to our business. To date, although our licensors have filed patent applications, we do not own or have any
rights to any issued patents that cover any of our product candidates, and we cannot be certain that we will secure any rights to any issued patents with
claims that cover any of our proprietary product candidates and technologies. The patent prosecution process is expensive and time-consuming and we 
may not be able to file and prosecute all necessary or desirable patent applications at a reasonable cost or in a timely manner. It is also possible that we
will fail to identify patentable aspects of our research and development output before it is too late to obtain patent protection. 

The patent process also is subject to numerous risks and uncertainties, and there can be no assurance that we will be successful in protecting our

products by obtaining and defending patents. These risks and uncertainties include the following: 

• Any patent rights, if obtained, might be challenged, invalidated, or circumvented, or otherwise might not provide any competitive advantage;
• Our  competitors,  many  of  which  have  substantially  greater  resources  than  we  do  and  many  of  which  might  make  significant  investments  in
competing technologies, might seek, or might already have obtained, patents that will limit, interfere with, or eliminate our ability to make, use,
and sell our potential products either in the U.S. or in international markets;

• As  a  matter  of  public  policy  regarding  worldwide  health  concerns,  there  might  be  significant  pressure  on  the  U.S.  government  and  other
international  governmental  bodies  to  limit  the  scope  of  patent  protection  both  inside  and  outside  the  U.S.  for  disease  treatments  that  prove
successful; and

• Countries other than the U.S. might have patent laws that provide less protection than those governing U.S. courts, allowing foreign competitors

the ability to exploit these laws to create, develop, and market competing products.

In addition, the U.S. Patent and Trademark Office and patent offices in other jurisdictions have often required that patent applications concerning
pharmaceutical and/or biotechnology-related inventions be limited or narrowed substantially to cover only the specific innovations exemplified in the
patent application, thereby limiting the scope of protection against competitive challenges. Thus, even if we or our licensors are able to obtain patents,
the patents might be substantially narrower than anticipated. 

Patent and other intellectual property protection is crucial to the success of our business and prospects, and there is a substantial risk that such
protections, if obtained, will prove inadequate. Our business and prospects will be harmed if we fail to obtain these protections or they prove insufficient.

If we fail to comply with our obligations under our license agreements, we could lose rights to our product candidates or key technologies. 

We have obtained rights to develop, market and sell some of our product candidates through intellectual property license agreements with third parties,
including IURTC and Therabiome. These license agreements impose various diligence, milestone payment, royalty and other obligations on us. If we
fail to comply with our obligations under our license agreements, we could lose some or all of our rights to develop, market and sell products covered by
these licenses, and our ability to form collaborations or partnerships may be impaired. In addition, disputes may arise under our license agreements with
third  parties,  which  could  prevent  or  impair  our  ability  to  maintain  our  current  licensing  arrangements  on  acceptable  terms  and  to  develop  and
commercialize the affected product candidates. 

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We may incur substantial costs as a result of litigation or other proceedings relating to patent and other intellectual property rights. 

If we choose to go to court to stop another party from using the inventions claimed in any patents we obtain, that individual or company has the
right to ask the court to rule that such patents are invalid or  should not  be enforced against that third  party. These lawsuits are expensive and would
consume time and resources and divert the attention of managerial and scientific personnel even if we were successful in stopping the infringement of
such patents. There is a risk that the court will decide that such patents are not valid and that we do not have the right to stop the other party from using
the inventions. There is also the risk that, even if the validity of such patents is upheld, the court will refuse to stop the other party on the ground that
such other party's activities do not infringe our rights to such patents. If we were not successful in defending our intellectual property, our competitors
could develop and market products based on our discoveries, which may reduce demand for our products. 

We  rely  on  trade  secret  protections  through  confidentiality  agreements  with  our  employees,  customers  and  other  parties,  and  the  breach  of  these
agreements could adversely affect our business and prospects. 

We  rely  on  trade  secrets  and  proprietary  know-how,  which  we  seek  to  protect,  in  part,  through  confidentiality,  invention,  and  non-disclosure
agreements  with  our  employees,  scientific  advisors,  consultants,  collaborators,  suppliers,  and  other  parties.  There  can  be  no  assurance  that  these
agreements will not be breached, that we would have adequate remedies for any such breach or that our trade secrets will not otherwise become known
to  or  independently  developed  by  our  competitors.  If  any  of  these  events  occurs,  or  we  otherwise  lose  protection  for  our  trade  secrets  or  proprietary
know-how, the value of this information may be greatly reduced. 

If our employees, or consultants breach their confidentiality obligations, to be able to enforce these confidentiality provisions, we would need to
know of the breach and have sufficient funds to enforce the provisions. We cannot assure you that we would know of or be able to afford enforcement of
any  breach.  In  addition,  such  provisions  are  subject  to  state  law  and  interpretation  by  courts,  which  could  limit  the  scope  and  duration  of  these
provisions. Any limitation on or non-enforcement of these confidentiality provisions could have an adverse effect on our business. 

We  may  infringe  the  intellectual  property  rights  of  others,  which  may  prevent  or  delay  our  product  development  efforts  and  stop  us  from
commercializing or increase the costs of commercializing our product candidates. 

Our success will depend in part on our ability to operate without infringing the proprietary rights of third parties. Our competitors may have filed,
and may in the future file, patent applications covering products and technologies similar to ours. Any such patent application may have priority over our
patent  applications,  which  could  further  require  us  to  obtain  rights  from  third  parties  to  issued  patents  covering  such  products  and  technologies.  We
cannot guarantee that the manufacture, use or marketing of any product candidates that we develop will not infringe third-party patents. 

A third party may claim that we are using inventions covered by the third party's patent rights and may go to court to stop us from engaging in our
normal operations and activities, including making or selling our product candidates. Patent litigation is costly and time consuming. We may not have
sufficient resources to address these actions, and such actions could affect our results of operations and divert the attention of managerial and scientific
personnel. 

If a patent infringement suit were brought against us, we may be forced to stop or delay developing, manufacturing, or selling potential products
that are claimed to infringe a third party's intellectual property, unless that third party grants us rights to use its intellectual property. In such cases, we
may be required to obtain licenses to patents or proprietary rights of others in order to continue development, manufacture or sale of our products. If we
are unable to obtain a license or develop or obtain non-infringing technology, or if we fail to defend an infringement action successfully, or if we are
found to have infringed a valid patent, we may incur substantial monetary damages, encounter significant delays in bringing our product candidates to
market and be precluded from manufacturing or selling our product candidates, any of which could harm our business significantly. 

Risks Related to Our Common Stock 

We might not be able to maintain the listing of our common stock on The NASDAQ Capital Market. 

Our common stock is listed on The NASDAQ Capital Market under the symbol “ASMB.” We might not be able to maintain the listing standards
of  that  exchange.  If  we  fail  to  maintain  the  listing  requirements,  our  common  stock  might  trade  on  the  OTC  Bulletin  Board  or  in  the  “pink  sheets”
maintained by Pink OTC Markets, Inc. These alternative markets are generally considered to be markets that are less efficient and less broad than The
NASDAQ Capital Market. 

25  
  
  
  
  
  
  
  
  
  
  
  
The price of our common stock might fluctuate significantly, and you could lose all or part of your investment. 

Since we went public on December 22, 2010 and through March 7, 2016, the closing price of our common stock has fluctuated between $4.30 and
$101.25 (after giving effect to the 1-for-5 reverse stock split effected on July 11, 2014), with significant volatility after we announced on June 25, 2012
that our prior product candidate iferanserin failed to meet the endpoints of our Phase III trial, and after we announced in February 2014 that our prior
product  candidate  diltiazem  demonstrated  no  significant  improvement  compared  to  placebo.  Continued  volatility  in  the  market  price  of  our  common
stock might prevent a stockholder from being able to sell shares of our common stock at or above the price paid for such shares. The trading price of our
common stock might be volatile and subject to wide price fluctuations in response to various factors, including: 

•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•

the receipt or loss of required regulatory approvals for our product candidates;
results of our preclinical studies and clinical trials and other studies involving our product candidates;
availability of capital;
future sales of our common stock;
sale of shares of our common stock by our significant stockholders or members of our management;
additions or departures of key personnel;
investor perceptions of us and the pharmaceutical industry;
issuance of new or changed securities analysts’ reports or recommendations, or the announcement of any changes to our credit rating;
success or failure of our product candidates;
introduction of new products or announcements of significant contracts, acquisitions or capital commitments by us or our competitors;
threatened or actual litigation and government investigations;
legislative, political or regulatory developments;
the overall performance of the equity markets;
actual or anticipated fluctuations in our quarterly financial and operating results;
general economic conditions;
changes in interest rates; and
changes in accounting standards, policies, guidance, interpretations or principles.

These and other factors might cause the market price of our common stock to fluctuate substantially, which might limit or prevent investors from
readily selling their shares of our common stock and might otherwise negatively affect the liquidity of our common stock. In addition, in recent years,
the stock market has experienced significant price and volume fluctuations. This volatility has had a significant impact on the market price of securities
issued by many companies across many industries. The changes frequently appear to occur without regard to the operating performance of the affected
companies. Accordingly, the price of our common stock could fluctuate based upon factors that have little or nothing to do with our company, and these
fluctuations could materially reduce our share price. 

Our principal stockholders and management own a significant percentage of our stock and will be able to exert significant control over matters 
subject to stockholder approval. 

At March 7, 2016, our executive officers, directors and one of our founders beneficially owned approximately 28.3% of our outstanding voting
common  stock,  and  this  group  together  with  other  stockholders  holding  beneficially  5%  of  more  of  our  outstanding  voting  common  stock,  owned
approximately 68.4% of our outstanding voting common stock Therefore, these stockholders, if acting together, have the ability to influence us through
their ownership position. These stockholders may be able to determine the outcome of certain significant matters requiring stockholder approval. For
example, these stockholders may be able to control elections of directors, amendments of our organizational documents, or approval of any merger, sale
of assets, or other major corporate transaction. This may prevent or discourage unsolicited acquisition proposals or offers for our common stock that you
may feel are in your best interest as one of our stockholders. 

We do not intend to pay dividends for the foreseeable future and our stock may not appreciate in value. 

We currently intend to retain our future earnings, if any, to finance the operation and growth of our business and do not expect to pay any cash
dividends in the foreseeable future. As a result, the success of an investment in shares of our common stock will depend upon any future appreciation in
its value. There is no guarantee that shares of our common stock will appreciate in value or that the price at which our stockholders have purchased their
shares will be able to be maintained. 

The requirements of being a public company add to our operating costs and might strain our resources and distract our management. 

As a public company, we face increased legal, accounting, administrative and other costs and expenses not faced by private companies. We are
subject  to  the  reporting  requirements  of  the  Securities  Exchange  Act  of  1934,  which  requires  that  we  file  annual,  quarterly  and  current  reports  with
respect  to  our  business  and  financial  condition,  and  the  rules  and  regulations  implemented  by  the  SEC,  the  Sarbanes-Oxley  Act  of  2002,  and  The 
NASDAQ Capital Market, each of which imposes additional reporting and other obligations on public companies. These rules and regulations increase
our legal and financial compliance costs and make some activities more time-consuming and costly, although we are currently unable to estimate these
costs with any degree of certainty. Complying with these requirements might divert management’s attention from other business concerns, which could 
have a material adverse effect on our prospects, business, and financial condition. 

26  
  
  
  
  
  
  
  
  
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Additionally, the expenses incurred by public companies generally for reporting and corporate governance purposes have been increasing. These
increased  costs  will  require  us  to  divert  a  significant  amount  of  money  that  we  could  otherwise  use  to  develop  our  product  candidates  or  otherwise
expand  our  business.  If  we  are  unable  to  satisfy  our  obligations  as  a  public  company,  we  could  be  subject  to  delisting  of  our  common  stock,  fines,
sanctions and other regulatory action and potentially civil litigation. 

Several  provisions  of  the  Delaware  General  Corporation  Law  and  our  Amended  and  Restated  Certificate  of  Incorporation  and  Bylaws  could
discourage, delay or prevent a merger or acquisition, which could adversely affect the market price of our securities. 

Several  provisions  of  the  Delaware  General  Corporation  Law  and  our  Amended  and  Restated  Certificate  of  Incorporation  and  Bylaws  could
discourage, delay or prevent a merger or acquisition that stockholders may consider favorable, and the market price of our securities could be reduced as
a result. These provisions may include: 

•

•
•
•

prohibiting  us  from  engaging  in  a  “business  combination” with  an  “interested  stockholder” for  a  period  of  three  years  after  the  date  of  the 
transaction in which the person became an interested stockholder unless certain provisions are met;
prohibiting cumulative voting in the election of directors;
limiting the persons who may call special meetings of stockholders; and
establishing advance notice requirements for nominations for election to our board of directors or for proposing matters that can be acted on by
stockholders at stockholder meetings.

If securities analysts downgrade our stock or cease coverage of us, the price of our stock could decline. 

The  trading  market  for  our  common  stock  relies  in  part  on  the  research  and  reports  that  industry  or  financial  analysts  publish  about  us  or  our
business. Currently, two financial analysts publish reports about us and our business. We do not control these or any other analysts. Furthermore, there
are many large, well-established, publicly traded companies active in our industry and market, which may mean that it is less likely that we will receive
widespread analyst coverage. If any of the analysts who cover us downgrade our stock, our stock price would likely decline rapidly. If these analysts
cease coverage of our company, we could lose visibility in the market, which in turn could cause our stock price to decline. 

Item 1B. Unresolved Staff Comments 

None. 

Item 2. Properties 

We lease office space for corporate and administrative functions in New York, NY under an agreement with a monthly lease payment of $10,130 that
expires in August 2016. We also lease office space in Carmel, IN under a lease agreement that expires in June 2021. The leased locations in New York,
NY and Carmel, IN are for corporate and administrative functions supporting both the HBV and Microbiome Programs and are adequate for our current
needs. 

We lease office and laboratory space in San Francisco, California under a sublease that expires in December 2017. We believe these leased facilities
are  adequate  for  our  current  needs  and  that  additional  space  will  be  available  in  the  future  on  commercially  reasonable  terms  as  needed.  Research
activities for the HBV program are also being conducted at laboratory space leased from Indiana University at Bloomington, IN. We believe these leased
facilities are adequate for our current needs. 

Item 3. Legal Proceedings 

We are not a party to any material legal proceedings at this time. From time to time, we may be subject to various legal proceedings and claims that
arise in the ordinary course of our business activities. Although the results of litigation and claims cannot be predicted with certainty, we do not believe
we  are  party  to  any  claim  or  litigation  the  outcome  of  which,  if  determined  adversely  to  us,  would  individually  or  in  the  aggregate  be  reasonably
expected to have a material adverse effect on our business. Regardless of the outcome, litigation can have an adverse effect on us because of defense and
settlement costs, diversion of management resources and other factors. 

Item 4. Mine Safety Disclosures 

Not applicable. 

27  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
 
 
 
 
PART II 

Item 5. Market for Registrant’s Common Equity, Related Stockholder Matters and Issuer Purchases of Equity Securities 

Market Information for Common Stock 

Our common stock is traded under the symbol “ASMB” and is quoted on the NASDAQ Capital Market. The following table sets forth the high and
low sales prices for shares of our common stock, as reported by NASDAQ for the periods indicated (after giving effect to the 1-for-5 reverse stock split 
effected on July 11, 2014). 

First quarter
Second quarter
Third quarter
Fourth quarter

2015

2014

High

Low

High

Low

$
$
$
$

17.00
20.50
19.91
11.49

$
$
$
$

7.25
12.08
9.22
7.22

$
$
$
$

23.45
8.30
9.68
9.47

$
$
$
$

6.10
4.25
6.40
6.51

On March 7, 2016, the closing price for the common stock as reported on the NASDAQ Capital Market was $6.00. 

Holders of Record 

As  of  March  7,  2016,  there  were  113  stockholders  of  record,  which  excludes  stockholders  whose  shares  were  held  in  nominee  or  street  name  by

brokers. 

Dividends 

We currently do not plan to declare dividends on shares of our common stock in the foreseeable future. We expect to retain our future earnings, if

any, for use in the operation and expansion of our business. 

Comparative Stock Performance Graph 

The information included under the heading “Comparative Stock Performance Graph” in this Item 5 of Part II of this Form 10-K shall not be deemed 
to be “soliciting material” or subject to Regulation 14A or 14C, shall not be deemed “filed” for purposes of Section 18 of the Exchange Act, or otherwise 
subject to the liabilities of that section, nor shall it be deemed incorporated by reference in any filing under the Securities Act or the Exchange Act. 

Set  forth  below  is  a  graph  comparing  the  total  cumulative  returns  of  our  common  stock,  the  NASDAQ  Composite  Index  and  the  NASDAQ
Biotechnology Index. The graph assumes $100 was invested in our common stock and each of the indices on December 31, 2010 and that all dividends,
if any, are reinvested. 

28  
  
  
  
  
  
  
  
  
  
  
  
  
 
 
  $
Assembly Biosciences, Inc.
NASDAQ Composite Index
  $
NASDAQ Biotechnology Index   $

Equity Compensation Plans 

12/31/2010   

100    $
100    $
100    $

12/31/2011
121
98.2
111.72

$
$
$

12/31/2012
32.63
113.82
147.61

$
$
$

12/31/2013   

57.7    $
157.44    $
244.35    $

12/31/2014
23.75
178.53
327.02

$
$
$

12/31/2015
22.69
188.75
364.82

The following table sets forth the indicated information as of December 31, 2015 with respect to our equity compensation plans : 

Number of 
securities to be  
issued upon  
exercise of  
outstanding  
options, warrants, 
and rights 
(a)

Weighted average 
exercise price of  
outstanding  
options, warrants, 
and rights  
(b) 

Number of 
securities  
remaining  
available for  
future issuance  
under equity  
compensation  
plans (excluding 
securities  
reflected in  
column (a)) 
(c)

2,479,666
266,467
621,651

$
$
$

16,909    $

3,384,693

9.56
8.14
2.22

30.81   

8,245
488,992
-

- 
497,237

Plan Category
Equity compensation plans approved by our stockholders:

2014 Stock Incentive Plan
2010 Equity Incentive Plan
Option assumed in Assembly Pharmaceuticals Merger

Equity compensation plans not approved by our stockholders:

Consultant Warrants

Total

29  
 
  
  
  
  
 
 
 
 
 
 
 
 
Our equity compensation plan consists of the 2014 Stock Plan and 2010 Plan which were approved by our stockholders. Our equity compensation
arrangements  that  have  not  been  approved  by  our  stockholders  consist  of  warrants  to  purchase  shares  of  our  common  stock  issued  to:  Paramount
BioCapital as placement agent in our 2008 common stock offering; S.L.A. Pharma to whom we issued a warrant for 13,605 shares of common stock as
part  of  an  amendment  to  the  license  agreement  between  us  and  S.L.A.  Pharma  for  VEN  307  and  VEN  308;  three  consultants;  National  Securities
Corporation  as  placement  agent  in  our  2010  convertible  note  offering;  the  underwriters  of  our  initial  public  offering  and  warrants  issued  to  Torreya
Capital, our financial advisor in the Merger. 

Shelf Registration 

On December 30, 2015, we filed a registration statement on Form S-3 with the SEC using a “shelf” registration process, file number 333-208806,
which  became  effective  January  19,  2016.  Under  this  shelf  registration  process,  we  may  from  time  to  time  sell  any  combination  of  the  securities
described in the registration statement in one or more offerings for an aggregate offering price of up to $150,000,000. The amount to be registered under
the shelf registration consists of up to $150,000,000 of an indeterminate amount of common stock, preferred stock, debt securities, warrants and/or units.
There is also being registered under the shelf registration a currently indeterminate number of (i) shares of common stock or other securities of us as may
be  issued  upon  conversion  of,  or  in  exchange  for,  convertible  or  exchangeable  debt  securities  and/or  preferred  stock  registered  under  the  registration
statement,  or  (ii)  shares  of  preferred  stock,  common  stock,  debt  securities  or  units  as  may  be  issued  upon  exercise  of  warrants  registered  by  the
registration statement, as the case may be. We have not issued any securities under this registration statement. 

Item 6. Selected Financial Data 

The  following  selected  consolidated  financial  data  should  be  read  in  conjunction  with  “Management’s  Discussion  and  Analysis  of  Financial

Condition and Results of Operations” and the financial statements and the notes thereto included elsewhere in this report. 

(In thousands)
Balance Sheet Data:
Total assets
Total stockholders’ equity

Statement of Operations Data:
Operating expenses
Loss from operations
Interest income
Interest expense
Realized loss from marketable securities
Net loss
Unrealized loss on marketable securities
Loss per Shares Data:

Basic and dilutive loss per share data

2015

2014

December 31,
2013

2012

2011

$

$

$

$

133,744
118,742

$

71,225
58,571

$

27,132    $
24,494     

20,556
17,810

$

37,046
34,533

$

29,656
(29,656)
1,229
-
(27)
(28,454) $
(822)

$

23,956
(23,956)
167
-
-
(23,789) $
-

19,605    $
(19,605)    
201     
-     
-     
(19,404)   $
-     

$

24,855
(24,855)
65
-
-
(24,790) $
-

34,002
(34,002)
76
(419)
-
(34,345)
-

(1.81) $

(3.40) $

(5.00)   $

(9.74) $

(17.86)

Item 7. Management’s Discussion and Analysis of Financial Condition and Results of Operation 

The  following  discussion  of  our  financial  condition  and  results  of  operations  should  be  read  in  conjunction  with  our  consolidated  financial
statements  and  the  related  notes  thereto  and  other  financial  information  appearing  elsewhere  in  this  Form  10-K.  The  following  discussion  contains
forward-looking  statements  that  involve  risks  and  uncertainties.  Our  actual  results  could  differ  materially  from  those  expressed  or  implied  in  any
forward-looking statements as a result of various factors, including those set forth in this Form 10-K under “Item 1A. Risk Factors.” 

Overview 

We are a biotechnology company advancing two innovative platform programs: a new class of oral therapeutics for the treatment of hepatitis B virus
(HBV) infection and novel class of oral biological therapeutics, which are deigned to restore health to a dysbiotic microbiome. The company’s HBV-
cure program is aimed at increasing the current low cure rate for patients with HBV and is pursuing several drug candidates that inhibit multiple viral
targets throughout the HBV lifecycle. Assembly has discovered several novel core protein Allosteric Modulators (CpAMs), which are small molecules
that directly target and allosterically modulate a number of HBc functions. The Company’s Microbiome Program consists of a fully integrated platform 
that includes a robust strain identification and selection process, methods for strain isolation and growth under cGMP conditions, and a patent pending
delivery system, GEMICELTM, which allows for targeted oral delivery of live biologic and conventional therapies to the lower gastrointestinal, or GI
tract.  The  lead  program  from  this  platform  is  in  development  for  the  treatment  of  C.  difficile infections  (CDI).  Using  its  microbiome  platform,  the 
Company is developing additional product candidates. 

30  
  
  
  
  
  
  
  
  
  
 
 
   
    
      
 
      
      
      
The  target  of  our  HBV  program  is  to  develop  novel  drugs  that  achieve  higher  cure  rates  than  current  therapies.  To  achieve  this  goal,  we  are
developing a series of new compounds, known as core protein allosteric modulators, or CpAMs, with the potential to modulate the HBV core protein- -at 
multiple points in the viral lifecycle.   

Our Microbiome Program consists of a fully integrated platform that includes a robust strain identification and selection process, methods for strain
isolation  and  growth  under  cGMP  conditions,  and  a  patent  pending  delivery  system,  GEMICELTM,  which  allows  for  targeted  oral  delivery  of  live 
biologic as well as conventional therapies to the lower GI tract. The lead program from this platform, AB-M101, is in development for the treatment of 
CDI. 

On July 11, 2014, Assembly Biosciences merged with a private company Assembly Pharmaceuticals, Inc., which was founded in 2012. The Merger
resulted  in  a  shift  in  strategic  focus,  the  addition  of  a  new  lead  drug  development program  for  us,  and  changes  in  personnel.  In  connection  with  the
Merger, our Board of Directors and stockholders approved a 1-for-5 reverse stock split of our common stock. The reverse stock split became effective on
July 11, 2014. All share and per share amounts in the consolidated financial statements and notes thereto have been retroactively adjusted for all periods
presented  to  give  effect  to  this  reverses  stock  split,  including  reclassifying  an  amount  equal  to  the  reduction  in  par  value  of  common  stock  to  the
additional  paid-in  capital.  In  connection  with  the  Merger,  the  shares  of  common  stock  issued  and  outstanding  of  Assembly  Pharmaceuticals  were
converted into an aggregate of 4,008,848 shares of our common stock. Also pursuant to the terms of the Merger, the outstanding options to purchase
shares of Assembly Pharmaceuticals’ common stock were assumed by us and became exercisable for an aggregate of 621,651 shares of our common
stock. Effective upon the consummation of the Merger, Assembly Acquisition, Inc., our wholly owned subsidiary (the “Merger Sub”) was merged with 
and into Assembly Pharmaceuticals, with Assembly Pharmaceuticals being the surviving entity and becoming our wholly owned subsidiary. 

We accounted for the acquisition of Assembly Pharmaceuticals, Inc. as a business combination under Accounting Standards Codification (“ASC”)
805 with Ventrus Biosciences, Inc. as the accounting acquirer. We determined Ventrus Biosciences, Inc. was the accounting acquirer in accordance with
ASC  805-10-25-5  as  Ventrus  Biosciences,  Inc.  gained  control  of  Assembly  Pharmaceuticals,  Inc.  upon  completion  of  the  Merger.  To  make  this
determination, we considered factors as indicated in ASC 805-10-55, including which entity issued equity interest to effect the combination, board of
directors’  composition,  shareholder  ownership,  voting  control,  restrictions  on  shareholder  voting  rights,  anticipated  management  positions  and  the
relative size of the two companies. 

We have not derived any revenue from product sales to date as we currently have no approved products. We anticipate initiating clinical trials in the
second half of 2016 with our microbiome therapy for recurrent CDI and our lead antiviral compound for the treatment of HBV. Once a product has been
developed,  it  will  need  to  be  approved  for  sale  by  the  Federal  Food  and  Drug  Administration  (FDA)  or  applicable  foreign  regulatory  agency.  Since
inception, our operations have been financed primarily through the sale of equity securities, the proceeds from the exercise of warrants and stock options
and issuance of debt. We have incurred losses from operations and negative cash flows from operating activities since inception and expect to continue
to incur substantial losses for the next several years as we continue our product development efforts. Management believes we currently have sufficient
funds to meet our operational requirements for at least the next twelve months. If we cannot generate significant cash from our operations, we intend to
obtain any additional funding we require through strategic relationships, public or private equity or debt financings, grants or other arrangements. We
cannot assure such funding will be available on reasonable terms, if at all. 

We  currently  have  administrative  offices  in  Carmel,  Indiana  and  New  York,  New  York  and  research  facilities  in  Bloomington,  Indiana  and  San
Francisco, California. Research activities for the HBV program are also being conducted at Indiana University at Bloomington, under the aegis of Adam
Zlotnick, PhD, Assembly co-founder and head of our HBV Scientific Advisory Board. 

Since Assembly’s inception, we have had no revenue from product sales, and have funded our operations principally through debt financings prior to
our initial public offering in 2010 and through equity financings since then. Our operations to date have been primarily limited to organizing and staffing
our company, licensing our product candidates, discovery and developing our product candidates, establishing initial manufacturing capabilities for our
product candidates, maintaining and improving our patent portfolio and raising capital. We have generated significant losses to date, and we expect to
continue to generate losses as we continue to develop our product candidates. As of December 31, 2015, we had an accumulated deficit of $163,965,905.
Because we do not generate revenue from any of our product candidates, our losses will continue as we further develop and seek regulatory approval for,
and commercialize, our product candidates. As a result, our operating losses are likely to be substantial over the next several years as we continue the
development of our product candidates and thereafter if none is approved or successfully launched. We are unable to predict the extent of any future
losses or when we will become profitable, if at all. 

Financial Operations Overview 

Research and Development Expense 

Research  and  development  expenses  consist  primarily  of  costs  incurred  for  our  research  activities,  including  our  drug  discovery  efforts,  target

validation, lead optimization and the development of our product candidates, which include: 

• employee-related expenses including salaries, benefits, and stock-based compensation expense;
• expenses incurred under agreements with third parties, including contract research organizations, or CROs, that conduct research and development,

preclinical and clinical activities on our behalf and the cost of consultants;

• the cost of lab supplies and acquiring, developing, and manufacturing preclinical study materials; and

31  
  
  
  
  
  
  
  
  
  
  
• facilities, depreciation, and other expenses, which include direct and allocated expenses for rent and maintenance of facilities, insurance, and other

operating costs.

Research and development costs are expensed as incurred. Nonrefundable advance payments for goods or services to be received in the future for use
in  research  and  development  activities  are  deferred  and  capitalized.  The  capitalized  amounts  are  expensed  as  the  related  goods  are  delivered  or  the
services are performed. 

We use our employee and infrastructure resources across multiple research and development programs, and we allocate internal employee-related and
infrastructure  costs,  as  well  as  certain  third  party  costs,  to  each  of  our  programs  based  on  the  personnel  resources  allocated  to  such  program.  Our
research and development expenses, by major program, are outlined in the table below: 

HBV
Microbiome
Diltiazem
Stock- Based Compensation

YE 2014

YE 2015

2,536,377 $
1,559,136 $
3,913,887 $
2,707,337 $

10,810,517
4,296,309
(6,621)
3,257,732

$
$
$
$

Diltiazem  was  a  prior  product  candidate  that  the  Company  is  no  longer  developing.  Since  the  Merger  in  July  2014,  the  HBV  platform  and

Microbiome platform are the sole focus of our company. 

The successful discovery and development of our product candidates are highly uncertain. As such, at this time, we cannot reasonably estimate, or
know the nature, timing and estimated costs, of the efforts that will be necessary to complete the remainder of their development. We are also unable to
predict  when,  if  ever,  material  net  cash  inflows  will  commence  from  our  product  candidates.  This  is  due  to  the  numerous  risks  and  uncertainties
associated with developing medicines, including the uncertainty of: 

•
•
•
•
•
•
•
•

successful completion of preclinical development and initiation of clinical development for each of the HBV and microbiome programs;
establishing an appropriate safety profile with IND-enabling toxicology studies;
successful enrollment in, and completion of, clinical trials;
receipt of marketing approvals from applicable regulatory authorities;
establishing commercial manufacturing capabilities or making arrangements with third-party manufacturers;
obtaining and maintaining patent and trade secret protection and regulatory exclusivity for our product candidates;
launching commercial sales of the products, if and when approved, whether alone or in collaboration with others; and
maintaining a continued acceptable safety profile of the products following approval and wide use.

A change in the outcome of any of these variables with respect to the development of any of our product candidates would significantly change the

costs and timing associated with the development of that product candidate. 

Research  and  development  activities  are  central  to  our  business  model.  Product  candidates  in  later  stages  of  clinical  development  generally  have
higher  development  costs  than  those  in  earlier  stages  of  clinical  development,  primarily  due  to  the  increased  size  and  duration  of  later-stage  clinical 
trials.  We  expect  research  and  development  costs  to  increase  significantly  for  the  foreseeable  future  as  our  product  candidate  development  programs
progress. However, we do not believe that it is possible at this time to accurately project total program-specific expenses through commercialization. 
There are numerous factors associated with the successful commercialization of any of our product candidates, including future trial design and various
regulatory  requirements,  many  of  which  cannot  be  determined  with  accuracy  at  this  time  based  on  our  stage  of  development.  Additionally,  future
commercial and regulatory factors beyond our control will impact our clinical development programs and plans. 

General and administrative expenses 

General  and  administrative  expenses  consist  primarily  of  salaries  and  other  related  costs,  including  stock-based  compensation,  for  personnel  in
executive, finance, accounting, business development, legal and human resources functions. Other significant costs include facility costs not otherwise
included in research and development expenses, legal fees relating to patent and corporate matters and fees for accounting and consulting services. 

We  anticipate  that  our  general  and  administrative  expenses  will  increase  in  the  future  to  support  continued  research  and  development  activities,
potential  commercialization  of  our  product  candidates  and  increased  costs  of  operating  as  a  public  company.  These  increases  will  likely  include
increased  costs  related  to  the  hiring  of  additional  personnel  and  fees  to  outside  consultants,  lawyers  and  accountants,  among  other  expenses.
Additionally, we anticipate increased costs associated with being a public company including expenses related to services associated with maintaining
compliance with exchange listing and SEC requirements, insurance, and investor relations costs. 

Interest income 

Interest income consists of interest earned on our cash and cash equivalents and available-for-sale securities. 

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Critical Accounting Policies and Significant Judgments and Estimates 

Our management’s discussion and analysis of our financial condition and results of operations are based on our consolidated financial statements,
which  we  have  prepared  in  accordance  with  accounting  principles  generally  accepted  in  the  U.S.  The  preparation  of  these  consolidated  financial
statements requires us to make estimates and assumptions that affect the reported amounts of assets and liabilities and the disclosure of contingent assets
and  liabilities  at  the  date  of  the  financial  statements,  as  well  as  the  reported  amounts  of  revenues  and  expenses  during  the  reporting  periods.  On  an
ongoing  basis,  we  evaluate  our  estimates  and  judgments,  including  those  described  in  greater  detail  below.  We  base  our  estimates  on  historical
experience and on various other factors that we believe are reasonable under the circumstances, the results of which form the basis for making judgments
about the carrying value of assets and liabilities that are not readily apparent from other sources. Actual results may differ from these estimates under
different assumptions or conditions. 

While our significant accounting policies are described in more detail in the notes to our consolidated financial statements included elsewhere in this
Annual Report on Form 10-K, we believe that the following accounting policies are the most critical to aid you in fully understanding and evaluating our
financial condition and results of operations. 

Marketable Securities 

We have designated marketable securities as of December 31, 2015 as available-for-sale securities and measure these securities at their respective fair
values.  Marketable  securities  are  classified  as  short-term  or  long-term  based  on  the  maturity  date  and  their  availability  to  meet  current  operating
requirements.  Marketable  securities  that  mature  in  one  year  or  less  are  classified  as  short-term  available-for-sale  securities  and  are  reported  as  a
component of current assets. Marketable securities that are not considered available for use in current operations are classified as long-term available-
for-sale securities and are reported as a component of long-term assets. 

Securities  that  are  classified  as  available-for-sale  are  measured  at  fair  value  with  temporary  unrealized  gains  and  losses  reported  in  other
comprehensive  income  (loss),  and  as  a  component  of  stockholders'  equity  until  their  disposition.  We  review  all  available-for-sale  securities  at  each 
period end to determine if they remain available-for-sale based on then current intent and ability to sell the security if it is required to do so. 

Marketable  securities  are  subject  to  a  periodic  impairment  review.  We  may  recognize  an  impairment  charge  when  a  decline  in  the  fair  value  of

investments below the cost basis is determined to be other-than-temporary. 

Goodwill and Other Intangible Assets 

Goodwill is the excess of purchase price over the fair value of identified net assets of businesses acquired. Our intangible assets with an indefinite
life are related to in-process research and development ("IPR&D") programs acquired in the Merger, as we expect future research and development on
these programs to provide us with substantial benefit for a period that extends beyond the foreseeable horizon. Intangible assets with indefinite useful
lives are measured at their respective fair values as of the acquisition date. We do not amortize goodwill and intangible assets with indefinite useful lives.
Intangible assets related to IPR&D projects are considered to be indefinite lived until the completion or abandonment of the associated R&D efforts. If
and when development is complete, which generally occurs if and when regulatory approval to market a product is obtained, the associated assets would
be deemed finite lived and would then be amortized based on their respective estimated useful lives at that point in time. 

We review goodwill and indefinite-lived intangible assets at least annually for possible impairment. Goodwill and indefinite-lived intangible assets 
are reviewed for possible impairment between annual tests if an event occurs or circumstances change that would more likely than not reduce the fair
value of the reporting unit or the indefinite-lived intangible assets below their carrying values. We test goodwill and indefinite-lived intangible assets 
each year on October 1. We review the carrying value of goodwill and indefinite-lived intangible assets utilizing a discounted cash flow model, and,
where appropriate, a market value approach is also utilized to supplement the discounted cash flow model. We make assumptions regarding estimated
future cash flows, discount rates, long-term growth rates and market values to determine each reporting unit’s estimated fair value. We considered the
adverse  changes  in  the  overall  biotechnology  sector  and  decline  in  our  stock  price  to  be  triggering  events  as  of  December 31,  2015.   Therefore,  in
addition to our annual goodwill impairment test as of October 1, 2015, we also performed a goodwill impairment test as of December 31, 2015.  As of
October 1, 2015 and December 31, 2015, the fair value of the Company’s reporting unit was in excess of carrying value for all scenarios that were tested.

Accrued Research and Development Expenses 

As part of the process of preparing our consolidated financial statements, we are required to estimate our accrued expenses. This process involves
reviewing quotations and contracts, identifying services that have been performed on our behalf and estimating the level of service performed and the
associated  cost  incurred  for  the  service  when  we  have  not  yet  been  invoiced  or  otherwise  notified  of  the  actual  cost.  The  majority  of  our  service
providers invoice us monthly in arrears for services performed or when contractual milestones are met. We make estimates of our accrued expenses as of
each balance sheet date in our financial statements based on facts and circumstances known to us at that time. 

Impairment of Long-lived Assets 

We monitor the carrying value of long-lived assets for potential impairment and test the recoverability of such assets whenever events or changes in
circumstances  indicate  that  the  carrying  amounts  may  not  be  recoverable.  If  a  change  in  circumstance  occurs,  we  perform  a  test  of  recoverability  by
comparing  the  carrying  value  of  the  asset  or  asset  group  to  its  undiscounted  expected  future  cash  flows.  If  cash  flows  cannot  be  separately  and
independently identified for a single asset, we will determine whether impairment has occurred for the group of assets for which we can identify the
projected cash flows. If the carrying values are in excess of undiscounted expected future cash flows, we measure any impairment by comparing the fair
value of the asset or asset group to its carrying value. We deemed there was no impairment of long-lived assets during the years ended December 31, 
2015 and 2014. 

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Fair Value Measurements 

We follow accounting guidance on fair value measurements for financial instruments measured on a recurring basis, as well as for certain assets and
liabilities that are initially recorded at their estimated fair values. Fair value is defined as the exit price, or the amount that would be received from selling
an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date. We use the following three-level 
hierarchy that maximizes the use of observable inputs and minimizes the use of unobservable inputs to value our financial instruments: 

Level 1: Observable inputs such as unadjusted quoted prices in active markets for identical instruments. 
Level 2: Quoted prices for similar instruments that are directly or indirectly observable in the marketplace. 
Level 3: Significant unobservable inputs which are supported by little or no market activity and that are financial instruments whose values are
determined  using  pricing  models,  discounted  cash  flow  methodologies,  or  similar  techniques,  as  well  as  instruments  for  which  the
determination of fair value requires significant judgment or estimation. 

Financial  instruments  measured  at  fair  value  are  classified  in  their  entirety  based  on  the  lowest  level  of  input  that  is  significant  to  the  fair  value
measurement. Our assessment of the significance of a particular input to the fair value measurement in its entirety requires us to make judgments and
consider  factors  specific  to  the  asset  or  liability.  The  use  of  different  assumptions  and/or  estimation  methodologies  may  have  a  material  effect  on
estimated fair values. Accordingly, the fair value estimates disclosed or initial amounts recorded may not be indicative of the amount that we or holders
of the instruments could realize in a current market exchange. 

Stock-Based Compensation 

We  apply  the  fair  value  recognition  provisions  of  Financial  Accounting  Standards  Board  (“FASB”)  ASC  Topic  718, Compensation-Stock
Compensation,  which  we  refer  to  as  ASC 718,  to  account  for  stock-based  compensation.  We  recognize  stock-based  compensation  expense related  to
stock options granted to employees and directors for their services on the Board of Directors based on the estimated fair value of each stock option on
the date of grant, net of estimated forfeitures, using the Black-Scholes option-pricing model. The grant date fair value of awards subject to service-based 
vesting, net of estimated forfeitures, is recognized on a straight-line basis over the requisite service period, which is generally the vesting period of the
respective  awards.  In  accordance  with  the  ASC  718,  stock  options  subject  to  both  performance-  and  service-based  vesting  conditions  are  recognized
using an accelerated recognition model if achievement of the performance requirements is considered to be probable. 

We account for stock options granted to non-employees, which primarily consist of consultants and members of our scientific advisory board, using
the fair value method. Stock options granted to non-employees are subject to periodic revaluation over their vesting terms and stock-based compensation 
expense is recognized using an accelerated recognition model. 

We use the Black-Scholes option pricing model to estimate the fair value of stock option awards using various assumptions that require management

to apply judgment and make estimates, including: 

  • the  expected  term  of  the  stock  option  award,  which  we  calculate  using  the  simplified  method,  as  prescribed  by  the  Securities  and  Exchange
Commission Staff Accounting Bulletin No. 107, Share-Based Payment, as we have insufficient historical information regarding our stock options to
provide a basis for an estimate;

  • the  expected  volatility  of  the  underlying  common  stock,  which  we  estimate  based  on  the  standard  deviation  of  our  underlying  stock  price's  daily
logarithmic  returns  since  December  17,  2010,  and  then  weighted  after  the  historical  volatility  of  a  representative  group  of  publicly  traded
biopharmaceutical companies with similar characteristics to us, including development candidates in earlier stages of drug development and areas of
therapeutic focus;

  • the risk-free interest rate, which we based on the yield curve of U.S. Treasury securities with periods commensurate with the expected term of the

options being valued;

  • the expected dividend yield, which we estimate to be zero based on the fact that we have never paid cash dividends and have no present intention to

pay cash dividends; and

  • the fair value of our common stock on the date of grant.

If factors change and different assumptions are used, our stock-based compensation expense could be materially different in the future. 

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In addition to the assumptions used in our Black-Scholes option-pricing model, the amount of stock option expense we recognize in our consolidated
statements of operations and comprehensive loss includes an estimate of stock option forfeitures. Under ASC 718, we are required to estimate the level
of forfeitures expected to occur and record compensation expense only for those awards that we ultimately expect will vest. Due to the lack of historical
forfeiture  activity,  we  expect  to  estimate  our  forfeiture  rate  based  on  data  from  our  representative  group  of  companies.  Changes  in  the  estimated
forfeiture rate can have a significant impact on our stock-based compensation expense as the cumulative effect of adjusting the rate is recognized in the
period the forfeiture estimate is changed. For example, if a revised forfeiture rate is higher than the previously estimated forfeiture rate, an adjustment is
made that will result in a decrease to the stock-based compensation expense recognized in the consolidated financial statements. If a revised forfeiture
rate is lower than the previously estimated forfeiture rate, an adjustment is made that will result in an increase to the stock-based compensation expense 
recognized in our consolidated financial statements. To date our forfeitures have not been material. 

Off-Balance Sheet Arrangements 

Since our inception, we have not engaged in any off-balance sheet arrangements, including the use of structured finance, special purpose entities or

variable interest entities. 

Contractual Obligations 

We  have  contractual  and  commercial  obligations  under  our  operating  leases  and  other  obligations  related  to  research  and  development  activities,
purchase commitments and licenses. The following table summarizes our future contractual obligations and commercial commitments at December 31,
2015. 

Operating leases
R&D and purchase commitments
License obligations
Total contractual obligations

Less than 1 year   
$

1,676,000    $
808,307     
425,000     
2,909,307    $

$

1-2 years

1,651,447
183,725
1,050,000 
2,885,172

In  general,  milestone  and  royalty  payments  associated  with  certain  license  agreements  have  not  been  included  in  the  above  table  of  contractual
obligations as we cannot reasonably estimate if or when they will occur. The milestone payments included in the table of contractual obligations above
are payments we believe are reasonably likely to occur during the indicated time periods. 

Results of Operations 

General 

To date, we have not generated any revenues from operations and, at December 31, 2015, we had an accumulated deficit of approximately $164.0
million primarily as a result of research and development expenses, purchases of in-process research and development and general and administrative 
expenses. While we may in the future generate revenue from a variety of sources, including license fees, milestone payments, research and development
payments in connection with strategic partnerships and/or product sales, our product candidates are at an early stage of development and may never be
successfully developed or commercialized. Accordingly, we expect to continue to incur substantial losses from operations for the foreseeable future and
there can be no assurance that we will ever generate significant revenues. 

Comparison of the Years Ended December 31, 2015 and December 31, 2014 

Research and Development Expense 

Research and development expense was $18,357,937 for the year ended December 2015, an increase of $7,641,200 or 71.3% from $10,716,737 for
the same period in 2014. The net increase in research and development expenses was primarily due to an increase of $8,274,140 in research expenses for
our HBV program which was started in July 2014, an increase of $2,737,173 for preclinical development of our Microbiome program, additional stock-
based compensation of $550,394 due to new options granted to employees and nonemployees, and offset by a decrease of $3,920,507 in expenses due to
termination of the VEN 307 study in the second quarter of 2014. 

General and Administrative Expense 

General and administrative expense was $11,297,693 for the year ended December 2015, a decrease of $1,942,022 or 14.7% from $13,239,715 for
the same period in 2014. The primary reason was a decrease of stock-based compensation expense of $3,311,304 due to the decrease of fair value of
stock  options  granted  to  employees  in  2015,  offset  by  an  increase  of  $1,369,282  in  general  and  administrative  expenses  related  to  compensation  and
benefits, severance payments payable over twelve months to our former chief executive officer, bonuses, professional fees and corporate taxes. 

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Interest Expense and Income 

There was no interest expense in 2015 or 2014. Interest income was $1,228,830 for the year ended 2015 compared to $167,653 for the same period in

2014. The interest income for the year ended December 31, 2015 was primarily related to the interest income on corporation bonds. 

Liquidity and Capital Resources 

As a result of our significant research and development expenditures and the lack of any FDA-approved products to generate product sales revenue, 
we have not been profitable and have generated operating losses since we were incorporated in October 2005. We have funded our operations through
December 31, 2015 principally with debt prior to our initial public offering, and thereafter with equity financing, raising an aggregate of $192.5 million
in net proceeds from public offerings and private placements from inception to December 31, 2015. 

In January 2014, we sold an aggregate of 92,472 shares of common stock under the amended at-the-market common equity sales program, resulting 

in net proceeds of approximately $1,763,000. 

On October 6, 2014, we sold to various institutional investors an aggregate of 1,959,000 shares of common stock in a registered direct offering. The
purchase price paid by the investors was $8.04 per share and an aggregate of approximately $14,963,000 in net proceeds were received. In connection
with  the  offering,  we  entered  into  a  placement  agent  agreement  with  William  Blair  &  Company,  L.L.C.,  who  acted  as  sole  placement  agent  in  the
offering, and pursuant to which we paid a placement agent fee equal to 5.0% of the gross proceeds of the offering. 

On March 19, 2015, we sold to various investors an aggregate of 5,555,555 shares of common stock in a public offering. The purchase price paid by
investors was $13.50 per share and an aggregate of $70.4 million (net of underwriting discounts and commissions and offering expenses) was received.
In addition, we granted the underwriters a 30-day option to purchase up to an additional 833,333 shares of common stock. 

On April 6, 2015, the underwriters exercised in full their option to purchase an additional 833,333 shares of common stock at the public offering
price of $13.50 per share, less underwriting discounts and commissions and offering expenses. Proceeds from the sale of shares on the exercise of the
underwriters’ option (net of underwriting discounts and commissions) were approximately $10.6 million. 

Cash Flows for the Two Years Ended December 31, 2015 and 2014 

(In thousands)
Statement of Cash Flows Data:
Total cash (used in)/provided by:
Operating activities
Investing activities
Financing activities

Net (decrease) increase in cash and cash equivalents

Net Cash Used in Operating Activities 

  For the Year Ended December 31, 

2015

2014

$

$

(18,697)   $
(64,855)    
81,569     

(14,974)
277
16,726 

(1,983)   $

2,029

Net cash used in operating activities was $18.7 million for the year ended December 31, 2015 and funded our research and development program
build out and general and administrative expenses. Net cash used in continuing operations for the year ended December 31, 2015 was primarily driven
by a $28.5 million net loss and offset by $7.9 million non-cash expense recorded for the stock-based compensation, plus a $1.8 million increase in cash 
from changes in operating assets and liabilities. Net cash used in continuing operations for the year ended December 31, 2014 was primarily driven by a
$23.8  million  net  loss  and  a  $2.5  million  decrease  in  cash  from  changes  in  operating  assets  and  liabilities,  and  offset  by  the  $10.6  million  non-cash 
expense recorded for stock-based compensation and $0.7 million non-cash expense recorded for the issuance of warrants for services. 

Net Cash (Used in) Provided by Investing Activities 

Net cash used  in  investing  activities  from continuing operations  for the  year ended  December 31, 2015 was  primarily due to a purchase of $69.8
million  of  marketable  securities  and  offset  by  a  $5.0  million  of  redemption  of  marketable  securities  during  the  year.  Net  cash  provided  by  investing
activities from continuing operations for the year ended December 31, 2014 for $0.3 million was primarily from cash acquired in business combination
of $0.5 million offset by purchase of $0.1 million of fixed assets and collection of $0.1 million of security deposits. 

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Net Cash Provided by Financing Activities 

Net cash flows provided by financing activities from continuing operations in the year ended December 31, 2015 was primarily generated by the net
proceeds of $81.0 million from our public offering and $0.6 million from the exercise of stock options to purchase 76,422 shares of common stock. Net
cash  provided  by  financing  activities  during  the  year  ended  December  31,  2014  consisted  of  the  sale  of  2,051,472  shares  of  common  stock  for  net
proceeds of $16.7 million. 

Funding Requirements 

We  expect  our  expenses  to  increase  in  connection  with  our  ongoing  activities,  particularly  as  we  continue  the  research,  development  and  clinical
trials  of  our  product  candidates.  Furthermore,  we  expect  to  continue  to  incur  additional  costs  associated  with  operating  as  a  public  company.
Accordingly, we will need to obtain substantial additional funding in connection with our continuing operations. If we are unable to raise capital when
needed or on attractive terms, we would be forced to delay, reduce or eliminate our research and development programs or future commercialization
efforts. 

We monitor our cash needs and the status of the capital markets on a continuous basis. From time to time, we opportunistically raise capital and have
done so numerous times since our initial public offering by issuing equity securities, most recently in March and April 2015. We expect to continue to
raise capital when and as needed and at the time and in the manner most advantageous to us. 

We expect that our existing cash, cash equivalents and marketable securities, will enable us to fund our operating expenses and capital expenditure

requirements for at least the next twelve months. Our future capital requirements will depend on many factors, including: 

•

•
•
•

•

the scope, progress, results and costs of drug discovery, preclinical development, laboratory testing and future clinical trials for our product
candidates;
the extent to which we acquire or in-license other medicines and technologies;
the costs, timing and outcome of regulatory review of our product candidates;
the costs of preparing, filing and prosecuting patent applications, maintaining and enforcing our intellectual property rights and defending
intellectual property-related claims; and
our ability to establish collaborations on favorable terms, if at all.

Identifying potential product candidates and conducting preclinical testing and clinical trials is a time-consuming, expensive and uncertain process 
that takes years to complete, and we may never generate the necessary data or results required to obtain marketing approval and achieve product sales. In
addition,  our  product  candidates,  if  approved,  may  not  achieve  commercial  success.  Our  commercial  revenues,  if  any,  will  be  derived  from  sales  of
medicines  that  we  do  not  expect  to  be  commercially  available  for  many  years,  if  at  all.  Accordingly,  we  will  need  to  continue  to  rely  on  additional
financing to achieve our business objectives. Adequate additional financing may not be available to us on acceptable terms, or at all. 

Until  such  time,  if  ever,  as  we  can  generate  substantial  product  revenues,  we  expect  to  finance  our  cash  needs  through  a  combination  of  equity
offerings, debt financings, collaborations, strategic alliances and licensing arrangements. We do not have any committed external source of funds. To the
extent that we raise additional capital through the sale of equity or convertible debt securities, the ownership interest of our stockholders will be diluted,
and  the  terms  of  these  securities  may  include  liquidation  or  other  preferences  that  adversely  affect  the  rights  of  our  common  stockholders.  Debt
financing,  if  available,  may  involve  agreements  that  include  covenants  limiting  or  restricting  our  ability  to  take  specific  actions,  such  as  incurring
additional debt, making capital expenditures or declaring dividends. 

If  we  raise  funds  through  additional  collaborations,  strategic  alliances  or  licensing  arrangements  with  third  parties,  we  may  have  to  relinquish
valuable  rights  to  our  technologies,  future  revenue  streams,  research  programs  or  product  candidates  or  to  grant  licenses  on  terms  that  may  not  be
favorable to us. If we are unable to raise additional funds through equity or debt financings when needed, we may be required to delay, limit, reduce or
terminate our product development or future commercialization efforts or grant rights to develop and market product candidates that we would otherwise
prefer to develop and market ourselves. 

Recent Accounting Pronouncements 

See Note 2 of notes to the consolidated financial statements for a discussion of recent accounting standards and pronouncements. 

Cautionary Statement 

We operate in a highly competitive environment that involves a number of risks, some of which are beyond our control. The following statement

highlights some of these risks. For more detail, see “Item 1A. Risk Factors.” 

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Statements  contained  in  this  Form  10-K  that  are  not  historical  facts,  are  or  might  constitute  forward-looking  statements  under  the  safe  harbor
provisions of the Private Securities Litigation Reform Act of 1995. Although we believe the expectations reflected in such forward-looking statements 
are based on reasonable assumptions, our expectations might not be attained. Forward-looking statements involve known and unknown risks that could 
cause actual results to differ materially from expected results. Factors that could cause actual results to differ materially from our expectations expressed
in the report include, among others: risks related to the costs, timing, regulatory review and results of our preclinical studies and clinical trials; our ability
to obtain FDA approval of our product candidates; our anticipated capital expenditures, our estimates regarding our capital requirements, and our need
for future capital; our liquidity and working capital requirements; our expectations regarding our revenues, expenses and other results of operations; the
unpredictability of the size of the markets for, and market acceptance of, any of our products; our ability to sell any approved products and the price we
are able realize; our ability to obtain future funding on acceptable terms; our ability to retain and hire necessary employees and to staff our operations
appropriately; our ability to compete in our industry and innovation by our competitors; our ability to stay abreast of and comply with new or modified
laws and regulations that currently apply or become applicable to our business; estimates and estimate methodologies used in preparing our financial
statements; the future trading prices of our common stock and the impact of securities analysts’ reports on these prices; and the risks set out in our filings 
with the SEC. 

Item 7A. Quantitative and Qualitative Disclosures about Market Risk 

Our primary exposure to market risk is interest income sensitivity, which is affected by changes in the general level of U.S. interest rates. 

We do not believe that our cash and equivalents have significant risk of default or illiquidity. Under our current investment policies, we invest our
cash and cash equivalents in money market funds which invest in short-term U.S. Treasury securities with insignificant rates of return. While we believe
our cash and equivalents do not contain excessive risk, we cannot provide absolute assurance that in the future our investments will not be subject to
adverse changes in market value. In addition, we maintain significant amounts of cash and equivalents at one or more financial institutions that are in
excess of federally insured limits. 

Inflation generally affects us by increasing our cost of labor and clinical trial costs. We do not believe that inflation has had a material effect on our

results of operations during 2015 or 2014. 

Item 8. Financial Statements and Supplementary Data 

The financial statements required to be filed pursuant to this Item 8 are appended to this report. An index of those financial statements is found on

page F-1. 

Item 9. Changes in and Disagreements with Accountants on Accounting and Financial Disclosure 

None. 

Item 9A. Controls and Procedures 

Evaluation of Disclosure Controls and Procedures 

We maintain a system of disclosure controls and procedures, as defined in Rule 13a-15(e) promulgated under the Securities and Exchange Act of 
1934, as amended (the “Exchange Act”), which is designed to provide reasonable assurance that information, which is required to be disclosed in our
reports  filed  pursuant  to  the  Exchange  Act,  is  accumulated  and  communicated  to  management  in  a  timely  manner.  At  the  end  of  fiscal  year  ending
December 31, 2015, we carried out an evaluation, under the supervision, and with the participation of, our management, including our Chief Executive
Officer and our Chief Financial Officer, of the effectiveness of the design and operation of our disclosure controls and procedures pursuant to Exchange
Act  Rule  13a-15(b).  Based  upon  that  evaluation,  our  Chief  Executive  Officer  and  Chief  Financial  Officer  concluded  that  our  disclosure  controls  and
procedures for the fiscal year ending as of December 31, 2015 were effective at reasonable assurance levels. 

Management’s Annual Report on Internal Control over Financial Reporting 

Our  management  is  responsible  for  establishing  and  maintaining  adequate  internal  control  over  financial  reporting,  as  such  term  is  defined  in
Exchange Act Rule 13a-15(f). Our internal control over financial reporting is designed to provide reasonable assurance to our management and Board of
Directors regarding the preparation and fair presentation of published financial statements. A control system, no matter how well designed and operated,
can only provide reasonable, not absolute, assurance that the objectives of the control system are met. Because of these inherent limitations, management
does not expect that our internal controls over financial reporting will prevent all error and all fraud. Under the supervision and with the participation of
our management, including our Chief Executive Officer and our Chief Financial Officer, we conducted an evaluation of the effectiveness of our internal
control  over  financial  reporting  based  on  the  framework  in  Internal  Control-Integrated  Framework issued  in  2013  by  the  Committee  of  Sponsoring 
Organizations of the Treadway Commission. Based on our evaluation under the framework in Internal Control-Integrated Framework, our management 
concluded that our internal control over financial reporting was effective as of December 31, 2015. 

Our independent registered public accounting firm, Ernst & Young LLP, has audited the financial statements included in this Form 10-K and has 
issued an unqualified opinion on the effectiveness of our internal control over financial reporting as of December 31, 2015. The report of Ernst & Young
LLP is included with the financial statements appended to this Form 10-K pursuant to Item 8. 

38  
  
  
  
  
  
  
  
  
  
  
  
  
  
Changes in Internal Control over Financial Reporting 

There were no changes in our internal control over financial reporting in the fourth quarter of 2015 that materially affected, or are reasonably likely

to materially affect, our internal control over financial reporting. 

Item 9B. Other Information 

Not applicable. 

PART III 

ITEM 10. Directors, Executive Officers and Corporate Governance

Except as set forth below, the information required by this item will be contained in our definitive proxy statement to be filed with the SEC in
connection with the Annual Meeting of Stockholders within 120 days after the conclusion of our fiscal year ended December 31, 2015, or the Proxy
Statement, and is incorporated in this Annual Report on Form 10-K by reference. 

Code of Ethics 

Our Board has adopted a Code of Ethics for our principal executive officer and all senior financial officers and a Code of Conduct applicable to all
of  our  employees  and  our  directors.  Both  Codes  are  available  under  the  Investor  Relations—Corporate  Governance  section  of  our  website  at 
www.assemblybio.com. If we make any substantive amendments to, or grant any waivers from, the Code of Ethics for our principal executive officer,
principal financial officer, principal accounting officer, controller or persons performing similar functions, or any officer or director, we will disclose the
nature of such amendment or waiver on our website or in a current report on Form 8-K. 

Section 16(a) Beneficial Ownership Reporting Compliance 

Pursuant to Section 16(a) of the Securities Exchange Act, our directors and executive officers are required to file reports with the SEC indicating
their holdings of and transactions in our equity securities. To our knowledge, based solely on our review of the copies of such reports furnished to us and
written representations that our officers, directors and holders of more than 10% of our common stock complied with all applicable filing requirements
during the fiscal year ended December 31, 2015. 

ITEM 11. Executive Compensation

The  information  required  by  this  item  will  be  contained  in  the  Proxy  Statement  and  is  incorporated  in  this  Annual  Report  on  Form  10-K  by 

reference. 

ITEM 12.

Security Ownership of Certain Beneficial Owners and Management and Related Stockholder Matters

Except for the table regarding equity compensation plans, which is included in Part II Item 5 of this Annual Report on Form 10-K, the information 

required by this item will be contained in the Proxy Statement and is incorporated in this Annual Report on Form 10-K by reference. 

ITEM 13. Certain Relationships and Related Party Transactions, and Director Independence

The  information  required  by  this  item  will  be  contained  in  the  Proxy  Statement  and  is  incorporated  in  this  Annual  Report  on  Form  10-K  by 

reference. 

ITEM 14.

Principal Accounting Fees and Services

The  information  required  by  this  item  will  be  contained  in  the  Proxy  Statement  and  is  incorporated  in  this  Annual  Report  on  Form  10-K  by 

reference. 

Item 15.

Exhibits, Financial Statement Schedules

(a) Exhibits.   The following exhibits are filed as part of this registration statement: 

39  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
Exhibit 
Number   Description of Document

1.1

3.1
3.2
3.3

4.1
4.2
4.3

4.4
4.5
4.6

4.7

4.8
4.9
10.1*

  Underwriting Agreement, dated March 19, 2015, by and Assembly Biosciences, 
Inc. and Credit Suisse Securities (USA) LLC and William Blair & Company, 
LLC, as representatives of the several underwriters named therein.

  Amended and Restated Certificate of Incorporation dated November 11, 2010.
  Amended and Restated Bylaws dated July 12, 2010.
  Certificate of Designation of Series A Non-Voting Convertible Preferred Stock of 

Ventrus Biosciences, Inc. filed on January 30, 2013.

  Specimen of Common Stock Certificate.
  Form of Warrant issued to investors between June and September 2008.
  Warrants issued to Paramount Credit Partners, LLC on January 23, March 25, 

June 1 and June 24, 2009.

  Form of Warrant issued to investors in February and March, 2010.
  Form of Warrant issued to investors in May 2010.
  Form of Placement Agent Warrant issued to Paramount BioCapital, Inc. on March 

11, 2008.

  Placement Agent Warrants issued to National Securities Corporation on February 
26, March 31 and May 6, 2010, as amended October 28, 2010 and November 30, 
2010.

  Warrant issued to S.L.A. Pharma AG on August 30, 2010.
  Form of underwriters warrant dated December 22, 2010.
  Exclusive License Agreement dated March 23, 2007 by and between S.L.A. 
Pharma AG and Paramount Biosciences, LLC, as amended on July 24, 2008, 
November 20, 2008, June 1, 2009, December 18, 2009 and June 24, 2010 and 
letter agreements dated October 27, 2008, November 20, 2008 and January 22, 
2009.

Registrant’s 
Form
8-K

Dated
  03/19/2015  

Exhibit
No.
1.6

Filed 
Herewith

S-1/A
S-1
8-K

S-3
S-1
S-1/A

S-1/A
S-1/A
S-1

  11/16/2010
  07/20/2010
  01/30/2013

  12/30/2015
  07/20/2010
  10/04/2010  

3.1
3.2
4.14

4.1
4.3
4.5

  10/04/2010
  10/04/2010
  07/20/2010  

4.8
4.9
4.10

S-1/A

  12/06/2010  

4.11

S-1/A
S-1/A
S-1/A

  10/04/2010
  12/06/2010
  11/16/2010  

4.12
4.13
10.1

10.2

  Assignment and Assumption Agreement dated August 2, 2007, by and between 

Paramount Biosciences LLC and Ventrus Biosciences, Inc.

10.3

  Amended and Restated Employment Agreement dated July 19, 2010 by and 

S-1

8-K

  07/20/2010

  07/20/2010

10.2

10.5

between Russell H. Ellison and Ventrus Biosciences, Inc.

10.4

  Amendment No. 6, dated August 30, 2010, to Exclusive License Agreement by 
and between S.L.A. Pharma AG and Paramount Biosciences, LLC (assigned to 
Ventrus Biosciences).

S-1/A

  10/04/2010

10.10

10.5

  Amendment No. 7, dated June 6, 2011, to Exclusive License Agreement by and 

S-1

  06/06/2011   10.16  

between S.L.A. Pharma AG and Paramount Biosciences, LLC (assigned to 
Ventrus Biosciences).

10.6

10.7

  Employment Agreement dated January 15, 2014 and effective December 22, 
2013, by and between Ventrus Biosciences, Inc. and Dr. Russell H. Ellison.
  Employment Agreement dated January 15, 2014 and effective December 22, 

2013, by and between Ventrus Biosciences, Inc. and David J. Barrett.

8-K

8-K

  01/16/2014   10.20  

  01/16/2014   10.21  

10.8*

  License and Collaboration Agreement dated November 8, 2013, by and between 

10-K

  03/31/2014   10.22  

10.9#

10.10#

10.11#

10.12#

Ventrus Biosciences, Inc. and Therabiome, LLC.

  Amendment dated July 11, 2014, to Employment Agreement, effective as of 

December 22, 2013 between Ventrus Biosciences, Inc. and Russell H. Ellison.
  Employment Agreement, dated July 11, 2014, between Ventrus Biosciences, Inc. 

and Derek A. Small.

  Employment Agreement, dated July 11, 2014, between Ventrus Biosciences, Inc. 

and Uri A. Lopatin.

  Employment Agreement, dated July 11, 2014, between Ventrus Biosciences, Inc. 

and Lee D. Arnold

8-K

8-K

8-K

8-K

  07/14/2014   10.23  

  07/14/2014

10.24

  07/14/2014

10.25

  07/14/2014

10.26

40 
 
 
 
 
   
 
   
 
   
 
 
 
 
 
 
 
 
 
 
 
 
 
10.13*   Exclusive License Agreement with Indiana University Research and Technology 

10-Q

  11/17/2014   10.29  

Corporation

21.1
23.1
23.2
24.1
31.1

  List of Subsidiaries of Assembly Biosciences, Inc.
  Consent of Ernst & Young LLP, Independent Registered Public Accounting Firm.
  Consent of EisnerAmper LLP, Independent Registered Public Accounting Firm.
  Power of Attorney (included on signature page)
  Certification of the Chief Executive Officer Pursuant to Section 302 of the 

Sarbanes-Oxley Act of 2002.

31.2

  Certification of the Chief Financial Officer Pursuant to Section 302 of the 

Sarbanes-Oxley Act of 2002.

32.1

  Certification of the Chief Executive Officer Pursuant to 18 U.S. C. Section 1350 

as Adopted Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002.

32.2

  Certification of the Chief Financial Officer Pursuant to 18 U.S. C. Section 1350 as 

Adopted Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002.

101.INS   XBRL Instance Document.
101.SCH   XBRL Taxonomy Extension Schema Document.
101.CAL   XBRL Taxonomy Extension Calculation Linkbase Document.
101.LAB   XBRL Taxonomy Extension Label Linkbase Document.
101.PRE   XBRL Taxonomy Extension Presentation Linkbase Document.
101.DEF   XBRL Taxonomy Extension Definitions Linkbase Document.

X
X
X
X
X

X

X

X

*Certain  information  in  this  exhibit  has  been  omitted  and  filed  separately  with  the  Securities  and  Exchange  Commission  pursuant  to  a  confidential
treatment request.   
# Represents management compensatory plan, contract or arrangement. 

41  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
In  accordance  with  Section  13  or  15(d)  of  the  Securities  Exchange  Act,  the  Registrant  caused  this  report  to  be  signed  on  its  behalf  by  the

undersigned, thereunto duly authorized. 

SIGNATURES 

Date: March 11, 2016

ASSEMBLY BIOSCIENCES, INC.

By:
Name:
Title:

/s/ Derek Small
Derek Small
President and Chief Executive Officer

KNOW ALL PERSONS BY THESE PRESENTS, that each person whose signature appears below constitutes and appoints Derek Small and David J. 
Barrett, jointly and severally, his or her attorneys-in-fact, each with the power of substitution, for him or her in any and all capacities, to sign any 
amendments to this report, and to file the same, with exhibits thereto and other documents in connection therewith with the Securities and Exchange 
Commission, hereby ratifying and confirming all that each of said attorneys-in-fact, or his or her substitute or substitutes may do or cause to be done by 
virtue hereof. 

Pursuant to the requirements of the Securities Exchange Act of 1934, this report has been signed by the following persons on behalf of the registrant

in the capacities and on the dates indicated. 

Signature

  Title

/s/ Derek Small
Derek Small

  President, Chief Executive Officer and Director

(Principal Executive Officer)

/s/ David J. Barrett
David J. Barrett

  Chief Financial Officer and Chief Operating Officer

(Principal Financial and Accounting Officer)

/s/ Anthony E. Altig
Anthony E. Altig

/s/ Mark Auerbach
Mark Auerbach

/s/ Richard DiMarchi
Richard DiMarchi

/s/ Myron Z. Holubiak
Myron Z. Holubiak

/s/ Alan Lewis 
Alan Lewis 

/s/ William Ringo
William Ringo

  Director

  Director

  Director

  Director

Director 

  Director

  Date

  March 11, 2016

  March 11, 2016

  March 11, 2016

  March 11, 2016

  March 11, 2016

  March 11, 2016

March 11, 2016 

  March 11, 2016

42  
  
  
  
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
  
  
  
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
ASSEMBLY BIOSCIENCES, INC. 
FINANCIAL STATEMENTS 

Reports of Independent Registered Public Accounting Firms

Consolidated Balance Sheets as of December 31, 2015 and 2014

Consolidated Statements of Operations and Comprehensive Loss for the Years Ended December 31, 2015 and 2014

Consolidated Statements of Changes in Stockholders’ Equity for the Years Ended December 31, 2015 and 2014

Consolidated Statements of Cash Flows for the Years Ended December 31, 2015 and 2014

Notes to the Consolidated Financial Statements

Page 

F-2

F-5

F-6

F-7

F-8

F-9

F-1  
 
 
 
 
 
 
 
REPORT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM 

To the Board of Directors and Stockholders of 
Assembly Biosciences, Inc.   

We have audited the accompanying consolidated balance sheet of Assembly Biosciences, Inc. and Subsidiary as of December 31, 2015, and the related
consolidated statements of operations and comprehensive loss, changes in stockholders’ equity and cash flows for the year then ended. These financial 
statements are the responsibility of the Company's management. Our responsibility is to express an opinion on these financial statements based on our
audit. 

We conducted our audit in accordance with the standards of the Public Company Accounting Oversight Board (United States). Those standards require
that  we  plan  and  perform the  audit  to  obtain  reasonable  assurance  about whether  the  financial  statements are free of  material misstatement. An  audit
includes examining, on a test basis, evidence supporting the amounts and disclosures in the financial statements. An audit also includes assessing the
accounting principles used and significant estimates made by management, as well as evaluating the overall financial statement presentation. We believe
that our audit provides a reasonable basis for our opinion. 

In  our  opinion,  the  financial  statements  referred  to  above  present  fairly,  in  all  material  respects,  the  consolidated  financial  position  of  Assembly
Biosciences,  Inc.  and  Subsidiary  at  December  31,  2015,  and  the  consolidated  results  of  its  operations  and  its  cash  flows  for  the  year  then  ended  in
conformity with U.S. generally accepted accounting principles.   

We also have audited, in accordance with the standards of the Public Company Accounting Oversight Board (United States), Assembly Biosciences, Inc.
and  Subsidiary’s  internal  control  over  financial  reporting  as  of  December  31,  2015,  based  on  criteria  established  in  Internal  Control-Integrated 
Framework issued by the Committee of Sponsoring Organizations of the Treadway Commission (2013 framework) and our report dated March 11, 2016
expressed an unqualified opinion thereon. 

/s/Ernst & Young LLP 

New York, New York 
March 11, 2016 

F-2  
  
  
  
  
  
  
  
REPORT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM 

To the Board of Directors and Stockholders of 
Assembly Biosciences, Inc.   

We  have  audited  Assembly  Biosciences,  Inc.  and  Subsidiary’s  internal  control  over  financial  reporting  as  of  December  31,  2015,  based  on  criteria
established  in  Internal  Control—Integrated  Framework  issued  by  the  Committee  of  Sponsoring  Organizations  of  the  Treadway  Commission  (2013
framework)  (the  COSO  criteria).  Assembly  Biosciences  Inc.’s  management  is  responsible  for  maintaining  effective  internal  control  over  financial
reporting, and for its assessment of the effectiveness of internal control over financial reporting included in the accompanying Management’s Annual 
Report  on  Internal  Control  over  Financial  Reporting.  Our  responsibility  is  to  express  an  opinion  on  the  company’s  internal  control  over  financial 
reporting based on our audit. 

We conducted our audit in accordance with the standards of the Public Company Accounting Oversight Board (United States). Those standards require
that we plan and perform the audit to obtain reasonable assurance about whether effective internal control over financial reporting was maintained in all
material respects. Our audit included obtaining an understanding of internal control over financial reporting, assessing the risk that a material weakness
exists, testing and evaluating the design and operating effectiveness of internal control based on the assessed risk, and performing such other procedures
as we considered necessary in the circumstances. We believe that our audit provides a reasonable basis for our opinion. 

A  company’s  internal  control  over  financial  reporting  is  a  process  designed  to  provide  reasonable  assurance  regarding  the  reliability  of  financial
reporting and the preparation of financial statements for external purposes in accordance with generally accepted accounting principles. A company’s 
internal control over financial reporting includes those policies and procedures that (1) pertain to the maintenance of records that, in reasonable detail,
accurately  and  fairly  reflect  the  transactions  and  dispositions  of  the  assets  of  the  company;  (2)  provide  reasonable  assurance  that  transactions  are
recorded as necessary to permit preparation of financial statements in accordance with generally accepted accounting principles, and that receipts and
expenditures  of  the  company  are  being  made  only  in  accordance  with  authorizations  of  management  and  directors  of  the  company;  and  (3)  provide
reasonable assurance regarding prevention or timely detection of unauthorized acquisition, use, or disposition of the company’s assets that could have a 
material effect on the financial statements. 

Because of its inherent limitations, internal control over financial reporting may not prevent or detect misstatements. Also, projections of any evaluation
of effectiveness to future periods are subject to the risk that controls may become inadequate because of changes in conditions, or that the degree of
compliance with the policies or procedures may deteriorate. 

In our opinion, Assembly Biosciences, Inc. and Subsidiary maintained, in all material respects, effective internal control over financial reporting as of
December 31, 2015, based on the COSO criteria. 

We also have audited, in accordance with the standards of the Public Company Accounting Oversight Board (United States), the consolidated balance
sheet of Assembly Biosciences, Inc. and Subsidiary as of December 31, 2015, and the related consolidated statements of operations and comprehensive
loss, changes in stockholders’ equity and cash flows for the year then ended of Assembly Biosciences, Inc. and Subsidiary and our report dated March
11, 2016 expressed an unqualified opinion thereon.  

/s/Ernst & Young LLP 

New York, New York 
March 11, 2016 

F-3  
  
  
  
  
  
  
  
  
  
REPORT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM 

To the Board of Directors and Stockholders 
Assembly Biosciences, Inc. 

We have audited the accompanying consolidated balance sheet of Assembly Biosciences, Inc. and subsidiary (the “Company”) as of December 31, 2014 
and the related consolidated statements of operations, changes in stockholders’ equity, and cash flows for the year then ended. The financial statements
are the responsibility of the Company’s management. Our responsibility is to express an opinion on these financial statements based on our audit. 

We conducted our audit in accordance with the standards of the Public Company Accounting Oversight Board (United States). Those standards require
that  we  plan  and  perform  the  audit  to  obtain  reasonable  assurance  about  whether  the  financial  statements  are  free  of  material  misstatement.  The
Company  is  not  required  to  have,  nor  were  we  engaged  to  perform,  an  audit  of  its  internal  control  over  financial  reporting.  Our  audits  included
consideration of internal control over financial reporting as a basis for designing audit procedures that are appropriate in the circumstances, but not for
the purpose of expressing an opinion on the effectiveness of the Company’s internal control over financial reporting. Accordingly, we express no such
opinion. An audit includes examining, on a test basis, evidence supporting the amounts and disclosures in the financial statements. An audit also includes
assessing  the  accounting  principles  used  and  significant  estimates  made  by  management,  as  well  as  evaluating  the  overall  financial  statement
presentation. We believe that our audit provides a reasonable basis for our opinion. 

In  our  opinion,  the  financial  statements  referred  to  above  present  fairly,  in  all  material  respects,  the  consolidated  financial  position  of  Assembly
Biosciences, Inc. and subsidiary as of December 31, 2014 and the consolidated results of their operations and their cash flows for the year then ended, in
conformity with accounting principles generally accepted in the United States of America. 

/s/ EisnerAmper LLP 

New York, New York 
March 12, 2015 

F-4  
  
  
  
  
  
  
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
CONSOLIDATED BALANCE SHEETS 

ASSETS
Current assets

Cash and cash equivalents
Marketable securities, at fair value
Other current assets

Total current assets

Long-term assets

Marketable securities
Property, plant and equipment, net
Security deposits
Intangible assets
Goodwill

Total long-term assets

Total assets

LIABILITIES AND STOCKHOLDERS' EQUITY
Current liabilities

Accounts payable
Accrued expenses

Total current liabilities

Long-term liabilities

Deferred tax liabilities

Total long-term liabilities

Total liabilities

Commitments and contingencies

As of December 31,

2015

2014

  $

$

27,107,526
40,556,652

704,287   
68,368,465   

29,091,113
-
125,284 
29,216,397 

23,392,129
148,609
197,158
29,000,000
12,638,136   
65,376,032   
133,744,497

1,363,698
2,039,204   
3,402,902   

$

$

-
156,441
115,005
29,000,000
12,737,350 
42,008,796 
71,225,193

907,601
146,420 
1,054,021 

  $

  $

11,600,000   
11,600,000   
15,002,902

11,600,000 
11,600,000 
12,654,021

Stockholders' equity
Preferred stock, $0.001 par value; 5,000,000 shares authorized; 0 shares issued and outstanding
Common stock, $0.001 par value; 50,000,000 shares authorized; 17,225,662 and 10,672,059 shares issued and 
outstanding at December 31, 2015 and December 31, 2014, respectively
Additional paid-in capital
Accumulated other comprehensive loss
Accumulated deficit
Total stockholders' equity
Total liabilities and stockholders' equity

-

-

17,226
283,511,859
(821,585)

(163,965,905)  
118,741,595   
133,744,497

$

  $

10,672
194,072,572
-
(135,512,072)
58,571,172 
71,225,193

The accompanying notes are an integral part of these consolidated financial statements 

F-5  
  
  
 
 
 
 
   
   
   
   
   
 
   
   
   
   
   
   
   
   
 
   
   
   
   
   
 
   
   
   
   
   
 
   
   
 
   
   
   
   
   
   
   
   
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
CONSOLIDATED STATEMENTS OF OPERATIONS AND COMPREHENSIVE LOSS 

Operating expenses:

Research and development
General and administrative

Total operating expenses
Loss from operations

Other income

Interest and other income
Realized loss from marketable securities

Total other income
Net loss

Unrealized loss on marketable securities
Comprehensive loss

Net loss per share, basic and diluted

Weighted average common shares outstanding, basic and diluted

Year Ended December 31,

2015

2014

  $

$

18,357,937
11,297,693
29,655,630
(29,655,630)

10,716,737
13,239,715
23,956,452
(23,956,452)

1,228,830

(27,033)  
1,201,797   
(28,453,833) $

167,653
- 
167,653 
(23,788,799)

(821,585)
(29,275,418) $

-
(23,788,799)

(1.81) $

(3.40)

15,702,646   

6,998,875 

  $

  $

  $

The accompanying notes are an integral part of these consolidated financial statements 

F-6  
  
 
 
 
 
   
   
   
   
 
   
   
   
   
   
 
   
   
 
   
 
   
   
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
CONSOLDATED STATEMENTS OF CHANGES IN STOCKHOLDERS’ EQUITY 

  Common Stock    Preferred Stock
  Shares
   4,146,779  $ 4,147    44,000 $

  Amount   Shares Amount Paid-in Capital

Issuable

Comprehensive Loss  

44 $

135,844,320 $

368,750 $

Additional

Common Stock Accumulated Other   Accumulated Total Stockholders'

Balance as of December 31, 2013

Proceeds from common stock sold, net of 
costs
Issuance of common stock for business 
combination
Issuance of common stock in exchange for 
restricted stock units
Conversion of preferred stock to common 
stock
Fair value of options assumed
Issuance of warrants for services
Stock-based compensation
Net loss

Balance as of December 31, 2014

Proceeds from common stock sold, net of 
underwriters' discounts and cost
Exercise of stock options
Cashless exercise of warrants
Stock-based compensation
Change in unrealized loss on marketable 
securities
Net loss

Balance as of December 31, 2015

   2,051,472   

2,051   

   4,008,808   

4,009   

25,000   

25   

-

-

-

-

-

-

16,723,895

29,060,139

-

-

368,725

(368,750)

440,000   
-   
-   
-   
-   
-   
-   
-   
-   
  10,672,059  $ 10,672   

440    (44,000)
-
-
-
-   
- $

(44)
-
-
-
-  
- $

(396)
758,948
679,447
10,637,494

-  
194,072,572 $

   6,388,888   
76,422   
88,293   
-   

6,389   
77   
88   
-   

-   
-   
-   
-   
  17,225,662  $ 17,226   

-
-
-
-

-
-   
- $

-
-
-
-

81,008,600
554,191
(88)
7,876,584

-
-  
- $

-
-  
283,511,859 $

-
-
-
-
-  
- $

-
-
-
-

-
-  
- $

Deficit
-  $(111,723,273) $

-   

-   

-   

-

-

-

-
-
-
-

-   
-   
-   
-   
-   
(23,788,799) 
-  $(135,512,072) $

-   
-   
-   
-   

-
-
-
-

-

(821,585)  
-   

(28,453,833) 
(821,585) $(163,965,905) $

Equity

24,493,988

16,725,946

29,064,148

-

-
758,948
679,447
10,637,494
(23,788,799)
58,571,172

81,014,989
554,268
-
7,876,584

(821,585)
(28,453,833)
118,741,595

The accompanying notes are an integral part of these consolidated financial statements 

F-7  
  
 
 
  
  
  
  
  
  
  
  
  
  
  
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
CONSOLIDATED STATEMENTS OF CASH FLOWS 

Cash flows from operating activities
Net loss
Adjustments to reconcile net loss to net cash used in operating activities:
Depreciation and amortization
Stock-based compensation
Realized loss from marketable securities
Loss on sale of fixed assets
Issuance of warrants for services
Changes in operating assets and liabilities:

Other current assets
Accounts payable
Accrued expenses
Security deposits

Net cash used in operating activities

Cash flows from investing activities

Purchase of fixed assets
Sale of fixed assets
Cash acquired in business combination
Security deposits collected
Purchases of marketable securities
Redemption of marketable securities

Net cash (used in) provided by investing activities

Cash flows from financing activities

Proceeds from common stock sold, net of underwriters' discounts and cost
Proceeds from exercise of stock options

Net cash provided by financing activities

Net (decrease) increase in cash and cash equivalents
Cash and cash equivalents at beginning of year
Cash and cash equivalents at end of year

Supplemental disclosure of cash flow information:
Cashless exercise of warrants
Change in unrealized loss on marketable securities available-for-sale
Issuance of common stock in exchange for restricted stock units
Conversion of preferred stock to common stock

Supplemental disclosure of non-cash activities:
Assembly business combination

Other current assets
Equipment, net
Intangible assets
Goodwill
Security deposits
Accounts payable and accrued expenses
Share exchange - business combination
Fair value of vested options and restricted stock units - in connection with business combination
Deferred tax liability

Cash acquired in business combination

Year Ended December 31,

2015

2014

  $

(28,453,833) $

(23,788,799)

64,989
7,876,584
27,033
954
-

(555,849)
456,097
1,968,844

(82,153)  

(18,697,334)

(58,261)
150
-
-
(69,781,176)

4,983,777   

(64,855,510)

81,014,989
554,268
81,569,257

(1,983,587)
29,091,113   
27,107,526

88
821,585
-
-

-
-
-
99,214
-
(99,214)
-
-
-   
-

$

$
$
$
$

$

$

10,974
10,637,494
-
-
679,447

(54,472)
(2,481,917)
23,771
- 
(14,973,502)

(149,963)
-
509,363
(81,999)
-
- 
277,401

16,725,946
-
16,725,946

2,029,845
27,061,268 
29,091,113

-
-
368,750
440

(23,540)
(10,350)
(29,000,000)
(12,737,350)
(16,606)
874,113
29,064,148
758,948
11,600,000 
509,363

  $

  $
  $
  $
  $

  $

  $

The accompanying notes are an integral part of these consolidated financial statements 

F-8  
  
 
 
 
 
   
   
   
   
   
   
   
   
   
   
   
   
   
 
   
   
   
   
   
   
   
   
   
 
   
   
   
   
   
 
   
   
   
 
   
   
 
   
   
   
   
   
   
   
   
   
   
   
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

Note 1 - Nature of Business 

Overview 

Assembly Biosciences, Inc. (“Assembly” or the “Company”) is a biotechnology company advancing two innovative platform programs: a new class of
oral  drugs  for  the  treatment  of  hepatitis  B  virus  (HBV)  infection  and  novel  class  of  oral  biological  drugs,  which  are  designed  to  restore  health  to  a
dysbiotic  microbiome.  The  Company’s  HBV-cure  program  is  aimed  at  increasing  the  current  low  cure  rates  for  patients  with  HBV  and  is  pursuing
multiple drug candidates that inhibit multiple viral targets throughout the HBV lifecycle. Assembly has discovered several novel core protein Allosteric
Modulators  (CpAMs),  which  are  small  molecules  that  directly  target  and  allosterically  modulate  a  number  of  HBc  functions.  The  Company’s 
Microbiome Program consists of a fully integrated platform that includes a robust strain identification and selection process, methods for strain isolation
and growth under cGMP conditions, and a patent pending delivery system, GEMICELTM, which allows for targeted oral delivery of live biologic as well 
as  conventional  therapies  to  the  lower  GI  tract.  The  lead  program  from  this  platform,  AB-M101,  is  in  development  for  the  treatment  of  C.  difficile-
infections (CDI). 

2015 Highlights 

On March 19, 2015, the Company sold to various investors an aggregate of 5,555,555 shares of common stock in a public offering. The purchase price 
paid by the investors was $13.50 per share and an aggregate of $70.4 million (net of underwriters’ discount and equity issuance costs) were received. 

On April 6, 2015, the underwriters exercised in full their option to purchase an additional 833,333 shares of common stock at the public offering price of
$13.50  per  share,  less  underwriting  discounts  and  commissions  and  offering  expenses.  Proceeds  from  the  sale  of  shares  on  the  exercise  of  the
underwriters’ option (net of underwriting discounts and commissions) were approximately $10.6 million. 

2014 Highlights 

On July 11, 2014, Nasdaq listed Ventrus Biosciences, Inc. caused its wholly owned subsidiary to merge with and into Assembly Pharmaceuticals, Inc., a
private  company (the “Merger”),  resulting  in Assembly Pharmaceuticals, Inc.  being  the surviving entity and  becoming  a  wholly  owned subsidiary of
Ventrus Biosciences, Inc. In connection with the Merger, Ventrus Biosciences, Inc. changed its name to Assembly Biosciences, Inc. The Merger resulted
in  a  shift  in  strategic  focus,  the  addition  of  a  new  lead  drug  development  program  and  changes  in  personnel.  In  connection  with  the  Merger,  the
Company’s Board of Directors and stockholders approved a 1-for-5 reverse stock split of the Company’s common stock. The reverse stock split became 
effective on July 11, 2014. All share and per share amounts in the consolidated financial statements and notes thereto have been retroactively adjusted
for all periods presented to give effect to this reverse stock split, including reclassifying an amount equal to the reduction in par value of common stock
to  additional  paid-in  capital.  In  connection  with  the  Merger,  the  shares  of common  stock  issued  and  outstanding  of  Assembly  Pharmaceuticals,  were
converted into an aggregate of 4,008,848 shares of the Company’s common stock. Also pursuant to the terms of the Merger, the outstanding options to
purchase  shares  of  Assembly  Pharmaceuticals’  common  stock  were  assumed  by  the  Company  and  became  exercisable  for  an  aggregate  of  621,651
shares of the Company’s common stock. 

The  Company  accounted  for  the  acquisition  of  Assembly  Pharmaceuticals,  Inc.  as  a  business  combination  under  Accounting  Standards  Codification
(“ASC”) 805 with Ventrus Biosciences, Inc. as the accounting acquirer. The Company determined Ventrus Biosciences, Inc. was the accounting acquirer
in accordance with ASC 805-10-25-5 as Ventrus Biosciences, Inc. gained control of Assembly Pharmaceuticals, Inc. upon completion of the Merger. To
make  this  determination,  the  Company  considered  factors  as  indicated  in  ASC  805-10-55,  including  which  entity issued  equity  interests  to  effect  the 
combination,  board  of  director  composition,  shareholder  ownership,  voting  control,  restrictions  on  shareholder  voting  rights,  anticipated  management
positions and the relative size of the two companies. 

On  October  6,  2014,  the  Company  sold  to  various  institutional  investors  an  aggregate  of  1,959,000  shares  of  common  stock  in  a  registered  direct
offering. The purchase price paid by the investors was $8.04 per share and an aggregate of $15.0 million in net proceeds were received. 

Liquidity 

The Company has not derived any revenue from product sales to date as it currently has no products. Once a product has been developed, it will need to
be approved for sale by the FDA or any foreign regulatory agency. Since inception, the Company’s operations have been financed primarily through the 
sale  of  equity  securities,  the  proceeds  from  the  exercise  of  warrants  and  stock  options  and  issuance  of  debt.  The  Company  has  incurred  losses  from
operations and negative cash flows since inception and expects to continue to incur substantial losses for the next several years as it continues its product
development efforts. Management believes the Company currently has sufficient funds to meet its operating requirements for at least the next twelve
months.  If  the  Company  cannot  generate  significant  cash  from  its  operations,  it  intends  to  obtain any  additional  funding  it  requires  through  strategic
relationships, public or private equity or debt financings, or other arrangements and it cannot assure such funding will be available on reasonable terms,
if at all. 

F-9  
  
  
  
  
  
  
  
  
  
  
  
Capital resources 

ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

The  Company  has  not  derived  any  revenue  from  product  sales  to  date  as  the  products  have  not  been  approved  for  sale  by  the  FDA  or  any  foreign
regulatory agency.  Since inception, the Company’s operations have been financed primarily through the sale of equity securities, the proceeds from the
exercise of warrants and stock options and issuance of debt.  The Company has incurred losses from operations and negative cash flows since inception
and expects to continue to incur substantial losses for the next several years as it continues its product development efforts. Management believes the
Company currently has sufficient funds to meet its operating requirements for the next twelve months. If the Company cannot generate significant cash
from its operations, it intends to obtain any additional funding it requires through strategic relationships, public or private equity or debt financings, or
other arrangements and it cannot assure such funding will be available on reasonable terms, or at all. 

Note 2 - Summary of Significant Accounting Policies and Recent Accounting Pronouncements 

Basis of Presentation 

The  accompanying  consolidated  financial  statements  include  the  accounts  of  the  Company  and  its  subsidiary.  All  intercompany  balances  and
transactions have been eliminated in consolidation. 

Segments 

The Company operates in one operating segment and, accordingly, no segment disclosures have been presented herein. 

Use of Estimates 

The preparation of financial statements in conformity with the accounting principles generally accepted in the United States of America (“U.S. GAAP”) 
requires management to make estimates and assumptions that may affect the reported amounts of assets and liabilities and disclosure of contingent assets
and liabilities at the date of the financial statements and reported amounts of expenses during the reporting period. Actual results could differ from those
estimates. 

Significant estimates inherent in the preparation of the accompanying financial statements include recoverability and useful lives (indefinite or finite) of
intangible assets, assessment of impairment of goodwill, and the fair value of stock options and warrants granted to employees, consultants, directors,
investors, licensors, placement agents and underwriters. 

The  Company’s  estimates  could  be  affected  by  external  conditions,  including  those  unique  to  the  Company  and  general  economic  conditions.  It  is
reasonably  possible  that  these  external  factors  could  have  an  effect  on  the  Company’s  estimates  and  could  cause  actual  results  to  differ  from  those 
estimates and assumptions. 

Reclassification 

Certain reclassifications have been made to prior year amounts to conform to the current year presentation. The Company reclassified certain non-cash 
activities in the consolidated statements of cash flows to conform to the Assembly Pharmaceutical’s final purchase price allocation. 

Cash and cash equivalents 

All highly liquid investments with maturities of three months or less at the time of purchase are considered to be cash equivalents.  All of the Company’s 
cash equivalents have liquid markets and high credit ratings.  The Company maintains its cash in bank deposit and other accounts, the balances of which,
at times and at December 31, 2015, exceed federally insured limits. 

Marketable Securities 

The  Company  has  designated  marketable  securities  as  of  December  31,  2015  as  available-for-sale  securities  and  measures  these  securities  at  their 
respective fair values. Marketable securities are classified as short-term or long-term based on the maturity date and their availability to meet current
operating requirements. Marketable securities that mature in one year or less are classified as short-term available-for-sale securities and are reported as 
a component of current assets. Marketable securities that are not considered available for use in current operations are classified as long-term available-
for-sale securities and are reported as a component of long-term assets. 

F-10  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

Securities that are classified as available-for-sale are measured at fair value with temporary unrealized gains and losses reported in other comprehensive
income (loss), and as a component of stockholders' equity until their disposition. The Company reviews all available-for-sale securities at each period 
end  to  determine  if  they  remain  available-for-sale  based  on  then  current  intent  and  ability  to  sell  the  security  if  it  is  required  to  do  so.  The  cost  of
securities sold is based on the specific identification method. 

Marketable securities are subject to a periodic impairment review. The Company may recognize an impairment charge when a decline in the fair value of
investments below the cost basis is determined to be other-than-temporary. 

Goodwill and Other Intangible Assets 

Goodwill is the excess of purchase price over the fair value of identified net assets of businesses acquired. The Company’s intangible assets with an 
indefinite life are related to in-process research and development ("IPR&D") programs acquired in the Merger, as the Company expects future research
and development on these programs to provide the Company with substantial benefit for a period that extends beyond the foreseeable horizon. Intangible
assets with indefinite useful lives are measured at their respective fair values as of the acquisition date. The Company does not amortize goodwill and
intangible assets with indefinite useful lives. Intangible assets related to IPR&D projects are considered to be indefinite lived until the completion or
abandonment of the associated R&D efforts. If and when development is complete, which generally occurs if and when regulatory approval to market a
product is obtained, the associated assets would be deemed finite lived and would then be amortized based on their respective estimated useful lives at
that point in time. 

The Company reviews goodwill and indefinite-lived intangible assets at least annually for possible impairment. Goodwill and indefinite-lived intangible 
assets are reviewed for possible impairment between annual tests if an event occurs or circumstances change that would more likely than not reduce the
fair value of the reporting unit or the indefinite-lived intangible assets below their carrying values. The Company tests its goodwill and indefinite-lived 
intangible  assets  each  year  on  October  1.  The  Company  reviews  the  carrying  value  of  goodwill  and  indefinite-lived  intangible  assets  utilizing  a
discounted  cash  flow  model,  and,  where  appropriate,  a  market  value  approach  is  also  utilized  to  supplement  the  discounted  cash  flow  model.  The
Company  makes  assumptions  regarding  estimated  future  cash  flows,  discount  rates,  long-term  growth  rates  and  market  values  to  determine  each 
reporting unit’s estimated fair value. The Company considered the adverse changes in the overall biotechnology sector and decline in the Company’s 
stock price to be triggering events as of December 31, 2015.  Therefore, in addition to the annual goodwill impairment test as of October 1, 2015, the
Company also performed a goodwill impairment test as of December 31, 2015.  As of October 1, 2015 and December 31, 2015, the fair value of the
Company’s reporting unit was in excess of carrying value for all scenarios that were tested. 

Impairment of Long-lived Assets 

The Company monitors the carrying value of long-lived assets for potential impairment and tests the recoverability of such assets whenever events or
changes in circumstances indicate that the carrying amounts may not be recoverable. If a change in circumstance occurs, the Company performs a test of
recoverability  by  comparing  the  carrying  value  of  the  asset  or  asset  group  to  its  undiscounted  expected  future  cash  flows.  If  cash  flows  cannot  be
separately  and  independently  identified  for  a  single  asset,  the  Company  will  determine  whether  impairment  has  occurred  for  the  group  of  assets  for
which the Company can identify the projected cash flows. If the carrying values are in excess of undiscounted expected future cash flows, the Company
measures any impairment by comparing the fair value of the asset or asset group to its carrying value. The Company performed its annual impairment
test as of October 1, 2015 and deemed there was no impairment of long-lived assets during the year ended December 31, 2015. 

Business Combinations 

The Merger (see Note 3) was made at a price above the fair value of the assets acquired and liabilities assumed including deferred tax liability, resulting
in  goodwill,  based  on  the  Company’s  expectations  of  synergies  and  other  benefits  of  combining  the  acquired  business.  These  synergies  and  benefits
include  elimination  of  redundant  functions  and  staffing  and  use  of  the  Company’s  existing  infrastructure  to  expand  development  of  the  product 
candidates of the acquired business in a cost efficient manner. 

Significant judgment is required in estimating the fair value of intangible assets and in assigning their respective useful lives. The fair value estimates are
based  on  available  historical  information  and  on  future  expectations  and  assumptions  deemed  reasonable  by  management,  but  which  are  inherently
uncertain. 

Net assets acquired are recorded at their fair values on the date of acquisition. 

Property and Equipment 

Property and equipment are stated at cost and consist of lab equipment and computer hardware and software. The Company computes depreciation under
the straight-line method over the following estimated useful life of the related assets: 

•
•

Lab equipment
Computer hardware and software

3 to 5 years
3 to 5 years

F-11  
  
  
  
  
  
  
  
  
  
  
  
  
  
Fair Value Measurements 

ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

The Company follows accounting guidance on fair value measurements for financial instruments measured on a recurring basis, as well as for certain
assets and liabilities that are initially recorded at their estimated fair values. Fair value is defined as the exit price, or the amount that would be received
from selling an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date. The Company use the
following  three-level  hierarchy  that  maximizes  the  use  of  observable  inputs  and  minimizes  the  use  of  unobservable  inputs  to  value  our  financial
instruments: 

Level 1: Observable inputs such as unadjusted quoted prices in active markets for identical instruments. 

Level 2: Quoted prices for similar instruments that are directly or indirectly observable in the marketplace. 

Level 3: Significant unobservable inputs which are supported by little or no market activity and that are financial instruments whose values are
determined  using  pricing  models,  discounted  cash  flow  methodologies,  or  similar  techniques,  as  well  as  instruments  for  which  the
determination of fair value requires significant judgment or estimation. 

Financial  instruments  measured  at  fair  value  are  classified  in  their  entirety  based  on  the  lowest  level  of  input  that  is  significant  to  the  fair  value
measurement. The assessment of the significance of a particular input to the fair value measurement in its entirety requires us to make judgments and
consider  factors  specific  to  the  asset  or  liability.  The  use  of  different  assumptions  and/or  estimation  methodologies  may  have  a  material  effect  on
estimated fair values. Accordingly, the fair value estimates disclosed or initial amounts recorded may not be indicative of the amount that the Company
or holders of the instruments could realize in a current market exchange. 

The  carrying  amounts  of  cash  equivalents  and  marketable  securities  approximate  their  fair  value  based  upon  quoted  market  prices.  Certain  of  the
Company’s financial instruments are not measured at fair value on a recurring basis, but are recorded at amounts that approximate their fair value due to
their liquid or short-term nature, such as cash, accounts receivable, accounts payable and accrued expenses. 

Stock-Based Compensation 

The  Company  expenses  stock-based  compensation  to  employees  over  the  requisite  service  period  based  on  the  estimated  grant-date  fair  value  of  the 
awards. For stock-based compensation awards to non-employees, the Company remeasures the fair value of the non-employee awards at each reporting
period prior to vesting and finally at the vesting date of the award. Changes in the estimated fair value of these non-employee awards are recognized as
compensation expense in the period of change. 

The Company estimates the fair value of stock option grants using the Black-Scholes option pricing model and the assumptions used in calculating the
fair  value  of  stock-based  awards  represent  management’s  best  estimates  and  involve  inherent  uncertainties  and  the  application  of  management’s 
judgment. 

Warrants 

For the purpose of valuing the warrants (see Note 8), the Company used the Black-Scholes option pricing model utilizing the assumptions for risk free
interest rate, the expected term, expected volatility, and expected dividend yield.  To determine the risk-free interest rate, the Company utilized the U.S. 
Treasury yield curve in effect at the time of grant with a term consistent with the expected term of the Company’s awards.  The Company estimated the
expected life of the warrants based on the full term of the warrant.   The expected dividend yield reflects the Company’s current and expected future 
policy  for  dividends  on  its  common  stock.  The  expected  stock  price  volatility  for  the  Company’s  stock  was  calculated  by  examining  historical 
volatilities for publicly traded industry peers as the Company did not have any trading history for its common stock at the time the grants were issued. 

Research and Development Costs 

Research and development costs are expensed as incurred. Research and development costs include salaries and personnel-related costs, consulting fees, 
fees  paid  for  contract  research  services,  fees  paid  to  clinical  research  organizations  and  other  third  parties  associated  with  clinical  trials,  the  costs  of
laboratory equipment and facilities, and other external costs. 

Income taxes 

The Company’s income tax expense consists of current and deferred income tax expense or benefit. Current income tax expense or benefit is the amount
of  income  taxes  expected  to  be  payable  or  refundable  for  the  current  year.  Deferred  tax  assets  and  liabilities  are  recognized  for  the  future  tax
consequences attributable to temporary differences between the financial statement carrying amounts of existing assets and liabilities and their respective
tax  bases.  Deferred  tax  assets  and  liabilities  are  measured  using  enacted  tax  rates  expected  to  apply  to  taxable  income  in  the  years  in  which  those
temporary differences are expected to be recovered or settled.  The effect on deferred tax assets and liabilities of a change in tax rates is recognized in
income  in  the  period  that  includes  the  enactment  date.  Valuation  allowances  are  established  when  it  is  more  likely  than  not  that  some  or  all  of  the
deferred tax assets will not be realized. 

F-12  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

The Company had adopted the provisions that tax positions must meet a “more-likely-than-not” recognition threshold to be recognized. The Company 
has  no  unrecognized  tax  benefits  recorded  for  the  years  ended  December  31,  2015  and  2014.  When  an  accrual  for  interest  and  penalties  is  required,
interest  and  penalties  will  be  recognized  in  tax  expense.  The  Company  files  income  tax  returns  in  the  U.S.  federal  jurisdiction  and  in  various  states.
There  are  currently  no  federal  income  tax  examinations  in  process.  Tax  years  beginning  in  2012  are  generally  subject  to  examination  by  taxing
authorities, although net operating losses from all years are subject to examinations and adjustments for at least three years following the year in which
the attributes are used. 

Loss per common share 

Basic net loss per common share excludes dilution and is computed by dividing net loss by the weighted average number of common shares outstanding
during the  period.  Diluted net  loss  per  common share reflects the potential  dilution  that could  occur  if  securities  or  other  contracts  to  issue common
stock were exercised or converted into common stock or resulted in the issuance of common stock that then shared in the earnings of the entity unless
inclusion of such shares would be anti-dilutive.  Since the Company has only incurred losses, basic and diluted net loss per share is the same.  Securities
that could potentially dilute loss per share in the future that was not included in the computation of diluted loss per share at December 31, 2015 and 2014
are as follows: 

Year Ended December 31,

Warrants to purchase common stock
Options to purchase common stock

Total

Comprehensive Loss 

2015

16,909     

2014
270,761
3,367,784      3,249,651 
3,384,693      3,520,412

Comprehensive  loss  is  comprised  of  net  loss  and  adjustments  for  the  change  in  unrealized  gains  and  losses  on  investments  in  available-for-sale 
marketable securities. The Company displays comprehensive loss and its components in the consolidated statements of operations and comprehensive
loss. 

Concentrations of Credit Risk 

Financial instruments  which potentially subject the Company to credit risk consist primarily  of cash, cash equivalents and marketable securities. The
Company  holds  these  investments  in  highly  rated  financial  institutions,  and,  by  policy,  limits  the  amounts  of  credit  exposure  to  any  one  financial
institution. These amounts at times may exceed federally insured limits. The Company has not experienced any credit losses in such accounts and does
not believe it is exposed to any significant credit risk on these funds. The Company has no off-balance sheet concentrations of credit risk, such as foreign 
currency exchange contracts, option contracts or other hedging arrangements. 

Recent Accounting Pronouncements 

In the first quarter of 2015, the Company adopted Accounting Standard Update (“ASU”) 2014-08, Reporting Discontinued Operations and Disclosures 
of  Disposals  of  Components  of  an  Entity  issued  by  the  Financial  Accounting  Standards  Board  (“FASB”).  ASU  2014-08  changes  the  definition  of  a 
discontinued operation to include only those disposals of components of an entity that represent a strategic shift that has (or will have) a major effect on
an entity's operations and financial results (e.g., a disposal of a major geographical area, a major line of business, a major equity method investment or
other  major  parts  of  an  entity). The  Company’s  adoption  of  ASU  2014-08  did  not  have  a  material  impact  on  the  Company’s  consolidated  financial 
statements. 

In  May  2014,  the  FASB  issued  ASU  2014-09,  Revenue  from  Contracts  with  Customers,  an  updated  standard  on  revenue  recognition. ASU  2014-09 
provides  enhancements  to  the  quality  and  consistency  of  how  revenue  is  reported  by  companies  while  also  improving  comparability  in  the  financial
statements of companies reporting using International Financial Reporting Standards or GAAP. The main purpose of the new standard is for companies
to recognize revenue to depict the transfer of goods or services to customers in amounts that reflect the consideration to which a company expects to be
entitled  in  exchange  for  those  goods  or  services.  The  new  standard  also  will  result  in  enhanced  disclosures  about  revenue,  provide  guidance  for
transactions  that  were  not  previously  addressed  comprehensively  and  improve  guidance  for  multiple-element  arrangements. In  July  2015,  the  FASB 
voted to approve a one-year deferral of the effective date of ASU 2014-09, which will be effective for the Company in the first quarter of fiscal year
2018 and may be applied on a full retrospective or modified retrospective approach. ASU 2014-09 will have no impact on the Company until it begins to 
generate revenue. 

F-13  
  
  
  
  
  
  
  
  
  
  
 
 
   
 
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

In June 2014, the FASB issued ASU 2014-10, Development Stage Entities (Topic 915) — Elimination of Certain Financial Reporting Requirements, 
Including an Amendment to Variable Interest Entities Guidance in Topic 810, Consolidation. The amendments in this update remove the definition of a 
development  stage  entity  from  the  Master  Glossary  of  the  Accounting  Standards  Codification,  thereby  removing  the  financial  reporting  distinction
between development stage entities and other reporting entities from GAAP. In addition, the amendments eliminate the requirements for development
stage  entities  to  (1)  present  inception-to-date  information  in  the  statements  of  income,  cash  flows  and  shareholder  equity,  (2)  label  the  financial
statements as those of a development stage entity, (3) disclose a description of the development stage activities in which the entity is engaged, and (4)
disclose in the first year in which the entity is no longer a development stage entity that in prior years it had been in the development stage. A public
entity  is  required  to  apply  the  amendments  for  annual  reporting  periods  beginning  after  December  15,  2014,  and  interim  periods  therein.  An  entity
should  apply  the  amendments  retrospectively  for  all  comparative  periods  presented.  Early  adoption  is  permitted.  The  guidance  was  adopted  by  the
Company in the second quarter of 2014. Adoption of this standard did not have a material impact on the Company’s consolidated financial statements. 

In  June  2014,  the  FASB  issued  ASU  2014-12,  Compensation-Stock  Compensation  (Topic  718).  ASU  2014-12  clarifies  how  entities  should  treat
performance  targets  that  can  be  achieved  after  the  requisite  service  period  of  a  share-based  payment  award.  The  accounting  standard  is  effective  for 
interim and annual periods beginning after December 15, 2015. ASU 2014-12 will have no impact on the Company until it begins to grant performance
awards. 

In August 2014, the FASB issued ASU 2014-15, Presentation of Financial Statements-Going Concern (Subtopic 205-40): Disclosure of Uncertainties 
about an Entity’s Ability to Continue as a Going Concern. ASU 2014-16 explicitly requires management to assess an entity’s ability to continue as a 
going concern, and to provide related footnote disclosures in certain circumstances. In connection with each annual and interim period, management will
assess  if  there  is  substantial  doubt  about  an  entity’s  ability  to  continue  as  a  going  concern  within  one  year  after  the  issuance  date.  Management  will
consider relevant conditions that are known, and reasonably knowable, at the issuance date. Substantial doubt exists if it is probable that the entity will
be unable to meet its obligations within one year after the issuance date. Disclosures will be required if conditions give rise to substantial doubt. The new
standard  will  be  effective  for  all  entities  in  the  first  annual  period  ending  after  December  15,  2016.  Early  adoption  is  permitted.  The  Company  is
currently evaluating the impact of this guidance on its consolidated financial statements. 

In November 2015, the FASB issued ASU No. 2015-17, Balance Sheet Classification of Deferred Taxes (“ASU 2015-17”). ASU 2015-17 requires that 
deferred  tax  liabilities  and  assets  be  classified  as  noncurrent  in  a  classified  statement  of  financial  position.  ASU  2015-17  is  effective  for  financial 
statements  issued  for  fiscal  years  beginning  after  December  15,  2016,  and  interim  periods  within  those  fiscal  years.  Early  adoption  is  permitted.
Adoption of this standard did not have a material impact on the Company’s consolidated financial statements. 

In January 2016, FASB issued ASU 2016-01, Recognition and Measurement of Financial Assets and Financial Liabilities. ASU 2016-01 requires equity 
investments to be measured at fair value with changes in fair value recognized in net income; simplifies the impairment assessment of equity investments
without  readily  determinable  fair  values  by  requiring  a  qualitative  assessment  to  identify  impairment;  eliminates  the  requirement  for  public  business
entities to disclose the method(s) and significant assumptions used to estimate the fair value that is required to be disclosed for financial instruments
measured at amortized cost on the balance sheet; requires public business entities to use the exit price notion when measuring the fair value of financial
instruments for disclosure purposes; requires an entity to present separately in other comprehensive income the portion of the total change in the fair
value  of  a  liability  resulting  from  a  change  in  the  instrument-specific  credit  risk  when  the  entity  has  elected  to  measure  the  liability  at  fair  value  in
accordance with the fair value option for financial instruments; requires separate presentation of financial assets and financial liabilities by measurement
category  and  form  of  financial  assets  on  the  balance  sheet  or  the  accompanying  notes  to  the  financial  statements  and  clarifies  that  an  entity  should
evaluate the need for a valuation allowance on a deferred tax asset related to available-for-sale securities in combination with the entity’s other deferred 
tax assets. ASU 2016-01 is effective for financial statements issued for fiscal years beginning after December 15, 2017, and interim periods within those
fiscal years. The Company is currently evaluating the impact that ASU 2016-01 will have on its financial statements and related disclosures. 

Note 3 - Assembly Pharmaceuticals, Inc. Transaction 

On July 11, 2014, the Company completed the Merger, whereby Assembly Pharmaceuticals became the Company’s wholly-owned subsidiary. Pursuant 
to the terms of the Merger, the shares of Assembly Pharmaceuticals were converted into an aggregate of 4,008,848 shares of the Company’s common 
stock. Also pursuant to the terms of the Merger, the options to purchase shares of Assembly Pharmaceuticals were assumed by the Company and became
exercisable for an aggregate of 621,651 shares of the Company’s common stock. 

F-14  
  
  
  
  
  
  
The allocation of the purchase price to the Assembly balance sheet is shown below: 

ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

Cash and cash equivalents
Other current assets
Equipment, net
IPR&D
Goodwill (as adjusted)
Security deposits
Total assets

Accrued expenses (as adjusted)
Deferred tax liability
Total liabilities
Net assets acquired

  $

  $

509,363
23,540
10,350
29,000,000
12,638,136
16,606 
42,197,995 

774,899
11,600,000
12,374,899
29,823,096

The transaction was accounted for using the acquisition method. Accordingly, goodwill has been measured as the excess of the total consideration over
the amounts assigned to the identifiable assets acquired and liabilities assumed including the related deferred tax liability. Goodwill is not deductible for
tax purposes. 

On  the  acquisition  date,  the  fair  value  of  net  assets  acquired  was  $29,823,096.  The  fair  value  of  stock  issued  to  the  Assembly  Pharmaceuticals’
shareholders as part of the consideration of $29,064,148 was based on reference to quoted market values of the Company’s common stock as of the date 
of acquisition. The options assumed in the Merger were valued at $758,948. As of June 30, 2015, the Company finalized its purchase price allocation.
The  Company  adjusted  certain  accrued  expenses,  resulting  in  a  decrease  of  goodwill  and  accrued  expenses  of  approximately  $99,000  in  the  second
quarter of 2015. 

Note 4 - Marketable Securities 

Marketable securities consist of the following as of December 31, 2015: 

Short-term available-for-sale securities

Corporate bonds

Long-term available-for-sale securities
Government and agency obligations
Municipal bonds
Corporate bonds

Total

Amortized Cost   

December 31, 2015
Gross Unrealized
Loss

Fair Value

  $

41,126,524    $
41,126,524     

(569,872)   $
(569,872)  

40,556,652 
40,556,652 

1,225,000     
1,596,160     
20,822,682     
23,643,842     
64,770,366    $

  $

(3,834)
(4,384)
(243,495)
(251,713)  
(821,585)   $

1,221,166
1,591,776
20,579,187
23,392,129 
63,948,781 

The contractual term to maturity of short-term marketable securities held by the Company as of December 31, 2015 is less than one year. The contractual
term to maturity of long-term marketable securities held by the Company as of December 31, 2015 is from 1 to 2 years. 

The fair value of marketable securities was classified into fair value measurement categories as follows: 

Quoted prices in active markets for identical assets (Level 1)
Quoted prices for similar assets observable in the marketplace (Level 2)
Significant unobservable inputs (Level 3)
Total

  $
-
    63,948,781
- 
  $ 63,948,781

The fair values of marketable securities are determined using quoted market prices from daily exchange traded markets based on the closing price as of
December 31, 2015 and are classified as Level 2. 

F-15  
  
  
  
  
  
  
  
  
  
  
   
   
   
   
   
   
 
   
   
   
   
 
 
     
 
 
 
     
     
 
 
   
There were no transfers between levels 1, 2 and 3 for the year ended December 31, 2015. 

ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

Note 5 - Goodwill and Intangible Assets 

Goodwill 

In July 2014, the Company completed its merger with Assembly Pharmaceuticals (see Note 3). The fair value of consideration paid, common stock and
assumed options, totaled $29,823,096, which, net of amounts allocated to assets and liabilities acquired at fair value, resulted in an allocation to goodwill
(as adjusted) of $12,638,136. The Company only has one operating segment. 

Goodwill is recorded as an indefinite-lived asset and is not amortized for financial reporting purposes but is tested for impairment on an annual basis or
when indications of impairment exist. No goodwill impairment losses have been recognized. Goodwill is not deductible for income tax purposes since
the tax basis is $0. The Company performed an impairment test of the carrying value of the Company’s goodwill at October 1, 2015 and December 31, 
2015 and deemed there was no goodwill impairment. 

The net book value of goodwill decreased by $99,214 from December 31, 2014 to December 31, 2015, as a result of the adjustment to purchase price
accounting within the measurement period. 

Intangible Assets 

In July 2014, the Company completed its acquisition of Assembly Pharmaceuticals (see Notes 1 and 3). The Company acquired in-process research and 
development  related  to  Assembly  Pharmaceuticals’  technology  which  is  an  indefinite  lived  intangible  asset.  The  Company  performed  its  annual
impairment test as of October 1, 2015 and deemed there was no impairment of long-lived assets during the year ended December 31, 2015. 

No intangible assets existed prior to the Merger. The change in intangible assets from July 11, 2014 to December 31, 2015 is shown in the table below: 

Merger on July 11, 2014
As of December 31, 2014

2015 activity

As of December 31, 2015

Note 6 - Property, Plant and Equipment, Net 

Property, plant and equipment, consists of the following: 

Computer hardware and software
Lab equipment
Office equipment
Total property, plant and equipment
Less: Accumulated depreciation and amortization
Property, plant and equipment, net

  $ 29,000,000
  $ 29,000,000
- 
  $ 29,000,000 

    Year Ended December 31,

Useful life (Years)   
3
3 to 5
3 to 5

    $

     $

2015

2014

84,065 $

177,782

1,109   
262,956   
(114,347)  
148,609 $

75,196
130,377
1,109 
206,682 
(50,241)
156,441

Depreciation expense for the years ended December 31, 2015 and 2014 was approximately $65,000 and $11,000, respectively, and was recorded in both
research and development expense and general and administrative expense in the consolidated statements of operations and comprehensive loss. There
was $883 of accumulated depreciation expense reversed due to the disposal of a fixed asset to Russell Ellison, the Company’s former CEO, in 2015. 

F-16  
  
  
  
  
  
  
  
  
  
  
  
  
  
   
 
 
 
     
   
     
   
      
   
      
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

Note 7 - Accrued Expenses 

Accrued expenses consist of the following: 

Accrued expenses:

Salaries, bonuses and employee benefits
Severance accrued for former CEO
Research and development expenses
Other

Total accrued expenses

Note 8 - Stockholders’ Equity 

Common and Preferred Stock Transactions 

2014 Activity 

Year Ended December 31,
2014
2015

$

  $

1,628,975    $
106,777     
120,700     
182,752     
2,039,204    $

-
-
-
146,420 
146,420 

In January 2014, the Company sold an aggregate of 92,472 shares of its common stock in its amended at-the-market common equity offering program,
resulting in net proceeds of approximately $1.8 million or $19.07 per share. 

In February 2014, all 44,000 outstanding shares of the Company’s Series A non-voting convertible preferred stock converted into an aggregate 440,000
shares of common stock. 

In July 2014, the Company issued 25,000 shares of common stock upon vesting of the restricted stock units. 

On  October  6,  2014,  the  Company  sold  to  various  institutional  investors  an  aggregate  of  1,959,000  shares  of  common  stock  in  a  registered  direct
offering. The purchase price paid by the investors was $8.04 per share and an aggregate of approximately $15.0 million in net proceeds were received. In
connection  with  the  offering,  the  Company  entered  into  a  placement  agent  agreement  with  William  Blair  &  Company,  L.L.C.,  who  acted  as  sole
placement agent in the offering, and pursuant to which the Company paid a placement agent fee equal to 5.0% of the gross proceeds of the offering. 

The Company’s Board of Directors and stockholders approved a 1-for-5 reverse stock split of the Company’s common stock. The reverse stock split
became effective on July 11, 2014. All share and per share amounts in the consolidated financial statements and notes thereto have been retroactively
adjusted  for  all  periods  presented  to  give  effect  to  this  reverse  stock  split,  including  reclassifying  an  amount  equal  to  the  reduction  in  par  value  of
common stock to additional paid-in capital. 

On July 11, 2014, the Company completed the Merger, whereby Assembly Pharmaceuticals became the Company’s wholly-owned subsidiary. Pursuant 
to  the  terms  of  the  Merger,  the  shares  of  Assembly  Pharmaceuticals,  common  stock  issued  and  outstanding  were  converted  into  an  aggregate  of
4,008,848  shares  of  the  Company’s  common  stock.  Also  pursuant  to  the  terms  of  the  Merger,  the  options  to  purchase  shares  of  Assembly
Pharmaceuticals common stock issued and outstanding immediately prior to the Merger were assumed by the Company and became exercisable for an
aggregate of 621,651 shares of the Company’s common stock. The fully vested assumed options in the Merger were valued at $758,948 using the Black-
Scholes model. The fair value of the options was recorded as a component of stockholders’ equity. The fair value of the options was determined using 
the Black-Scholes model with the following assumptions: risk free interest rate 1.66% - 2.15%, volatility 97.33% - 102.8%, expected term 5 - 6.1 years, 
and no expected dividends. 

2015 Activity 

On March 19, 2015, the Company sold to various investors an aggregate of 5,555,555 shares of common stock in a public offering. The purchase price
paid by the investors was $13.50 per share and an aggregate of $70.4 million in net proceeds were received, after deducting underwriting discounts and
commissions  and  estimated  offering  expenses.  In  addition,  the  Company  granted  the  underwriters  a  30-day  option  to  purchase  up  to  an  additional 
833,333 shares of common stock. 

On April 6, 2015, the underwriters exercised in full their option to purchase an additional 833,333 shares of common stock at the public offering price of
$13.50  per  share,  less  underwriting  discounts  and  commissions  and  offering  expenses.  The  closing  of  the  option  exercise  resulted  in  net  proceeds  of
approximately  $10.6  million.  Exercise of  the underwriters’ option increased  the net proceeds (net of underwriting discounts and commissions)  of the
public offering, from $70.4 million to $81.0 million. 

F-17  
  
  
  
  
  
  
  
  
  
  
  
  
  
  
 
 
   
      
 
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

On December 30, 2015, the Company filed a registration statement on Form S-3 with the SEC using a “shelf” registration process, file number 333-
208806, which became effective January 19, 2016. Under this shelf registration process, the Company may from time to time sell any combination of the
securities described in the registration statement in one or more offerings for an aggregate offering price of up to $150,000,000. The Company has not
issued any securities under this registration statement as of this date. 

Options, Warrants and Restricted Stock Units 

Options 

The  Company  has  two  equity  incentive  plans  available  for  the  granting  of  equity  awards.  In  July  2010,  the  stockholders  approved  the  2010  Equity
Incentive  Plan,  under  which,  as  of  December  31,  2015,  there  were  outstanding  options  for  an  aggregate  of  266,467  shares  of  common  stock  and  an
aggregate of 488,992 shares available for grant. In July 2014, the stockholders approved the 2014 Stock Incentive Plan (the “2014 Plan”), under which, 
as of December 31, 2015, there were options for an aggregate of 2,479,666 shares of common stock outstanding and 8,245 shares available for grant. 

On  February  10,  2015,  the  Company’s  former  Chief  Executive  Officer,  Dr.  Russell  Ellison,  transitioned  to  service  as  a  consultant.  The  Company
accelerated  266,667 of  his  options  on  March  3,  2015  in  accordance with the original terms of  his employment  agreement.  The  corresponding charge
related to these options was also accelerated in the first quarter of 2015. The exercise period for Dr. Ellison’s vested options were also extended until the 
end of their term, or July 9, 2024 in accordance with the original terms of his employment agreement. The remainder of 266,666 unvested options were
forfeited in accordance with the original terms of his employment agreement. 

A summary of the Company’s option activity and related information for the year ended December 31, 2015 is as follows: 

Outstanding as of December 31, 2014
Granted
Exercised
Expired
Forfeited
Outstanding as of December 31, 2015
Options vested and exercisable

3,249,651 $
500,300
(76,422)
(11,800)
(293,945)
3,367,784 $
2,080,280 $

Total Intrinsic Value
5,187,924
-
22,348
-
-
3,971,205
2,538,210

6.26 $
13.02
7.25
7.20
7.26
7.16 $
6.40 $

Number of Shares

Weighted Average
Exercise Price

The Company expects that all outstanding unvested options will vest. The fair value of the options granted for the year ended December 31, 2015 and
2014, was based on the following assumptions: 

Exercise price
Expected stock price volatility
Risk-free rate of interest
Term (years)

Year Ended December 31,
2014
2015
$2.22 - $8.13
$7.88 - $16.55

88.62% - 95.55%     94.4% - 105.0%
1.65% - 2.57%
4.9 - 10.0

1.49% - 2.27%    

5.0 - 8.2

The  weighted  average  remaining  contractual  life  of  options  outstanding  at  December  31,  2015  is  approximately  8.7  years.  The  weighted  average
remaining contractual life of options currently exercisable at December 31, 2015 is approximately 8.5 years. 

F-18  
  
  
  
  
  
  
  
  
  
 
 
 
     
     
     
Stock-based compensation expenses for the years ended December 31, 2015 and 2014 were as follows: 

ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

Research and development
General and administrative
Total stock-based compensation expense

Warrants 

Year Ended December 31,
2014
2015

$

  $

3,257,732    $
4,618,852     
7,876,584    $

2,707,337
7,930,157
10,637,494 

In connection with the Company’s financings from 2007 to 2010, the Company issued warrants to investors and/or placement agents, as well as certain
consultants, to purchase shares of common stock. In connection with the Merger, the Company issued warrants to purchase up to 120,265 shares of its
common stock to its financial advisor for the Merger. The warrants were valued at $679,447 and expensed during the quarter ended September 30, 2014.

On April 17, 2015, the Company issued an aggregate of 88,293 shares of common stock from the cashless exercise of 120,265 warrants. The Company
did not receive any proceeds from this cashless exercise. 

During the year ended December 31, 2015, 133,587 warrants to purchase common stock expired. 

A summary of the Company’s warrant activity and related information is as follows: 

Outstanding as of December 31, 2014
Expired
Exercised
Outstanding as of December 31, 2015

Warrants

Weighted Average
Exercise Price

270,761    $
(133,587)    
(120,265)    
16,909    $

24.34
39.34
5.13 
30.81

The weighted average remaining contractual life of outstanding warrants at December 31, 2015 was approximately 4.4 years. 

 Note 9 - Income Taxes 

There was no current or deferred income tax provision for the years ended December 31, 2015 and 2014. 

The Company’s deferred tax assets as of December 31, 2015 and 2014 consist of the following: 

Deferred tax assets:

Net-operating loss carryforward
Stock-based compensation
In-Process R&D
R&D credit
Change in unrealized loss on marketable securities
Other

Total Deferred Tax Assets
Valuation allowance
Deferred Tax Asset, Net of Allowance

In-process research and development (Assembly Merger)

Deferred Tax Liability

As of December 31,

2015

2014

46,365,000    $
10,397,000     
5,282,000     
3,068,000     
368,000     
783,000     
66,263,000     
(66,263,000)    
-    $
11,600,000     
11,600,000    $

38,094,000
11,691,000
5,697,000
2,600,000
-
2,000 
58,084,000
(58,084,000)
- 
11,600,000 
11,600,000

$

  $

$

F-19  
  
  
  
  
  
  
  
  
  
  
  
  
 
 
   
 
   
 
 
 
   
      
 
 
 
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

The Company recognized a $11.6 million deferred tax liability in 2015 and 2014 as a result of the acquisition of Assembly Pharmaceuticals in July 2014.
Due to the acquisition, a temporary difference between the book fair value and the tax basis of the other in-process research and development acquired 
created deferred tax liability of $11.6 million and additional goodwill was recorded. 

At December 31, 2015, the Company had potentially utilizable gross Federal net operating loss carryforwards of approximately $105.0 million, State net
operating loss carry-forwards of approximately $98.3 million and research and development credit carry forward of approximately $3.1 million, all of
which expire between 2027 and 2035. During 2015, the Company reclassed approximately $2.9 million of prior period deferred tax assets related to non-
deductible incentive stock options to non-deductible expenses. This reclass resulted in a 10.2% decrease to the effective tax rate and a corresponding
10.2% decrease to the change in valuation allowance. 

Sections 382 and 383 of the Internal Revenue Rode of 1986 subject the future utilization of net operating losses and certain other tax attributes to an
annual limitation in the event of certain ownership changes, as defined. The Company has undergone an ownership change study and has determined that
a “change in ownership” as defined by IRC Section 382 of the Internal Revenue Code of 1986, as amended, and the rules and regulations promulgated
thereunder, did occur in December 2010, January 2013 and October 2014. Accordingly, approximately $39.1million of the Company’s net operating loss 
carryforwards  are  limited.  Based  on  the  company  having  undergone  multiple  ownership  changes  throughout  their  history  these  net  operating  loss
carryforwards will free up at varying rates each year. 

The following is a reconciliation of the U.S. federal statutory rate to the effective income tax rates for the year ended December 31, 2015 and 2014: 

Statutory Federal Income Tax Rate
State Taxes, Net of Federal Tax Benefit
Merger Cost
Stock based Compensation - ISOs
Other
Change in Valuation Allowance
Income Taxes Provision (Benefit)

Note 10 - License Agreements 

HBV Research Agreement with Indiana University 

As of December 31,

2015

2014

(34.0)%  
(11.0)%  
0.9%   
16.7%   
0.1%   
27.3%   
0.0%   

(34.0)%
(11.0)%
0.0%
0.0%
0.0%
45.0%
0.0%

The Company, through its wholly-owned subsidiary, Assembly Pharmaceuticals, is party to a license agreement with Indiana University Research and
Technology Corporation  (“IURTC”)  from whom  it has  licensed  the Company’s HBV therapy. The  license  agreement  requires  the Company to make
milestone payments based upon the successful accomplishment of clinical and regulatory milestones related to the HBV therapy. The total amount of all
potential future milestone payments at December 31, 2015 is $825,000. The Company also is obligated to pay IURTC royalty payments based on net
sales of the licensed technology. The Company is also obligated to pay diligence maintenance fees (starting at $25,000 in 2014 and rising to $100,000 in
the year following first commercial sale of licensed product) each year to the extent that the royalty, sublicensing, and milestone payments to IURTC are
less than the diligence maintenance fee for that year. 

Microbiome Targeted Colonic Delivery Platform 

On November 8, 2013, Assembly entered into a License and Collaboration Agreement with Therabiome, LLC, for all intellectual property and know-
how  owned  or  controlled  by  Therabiome  relating  to  the  oral  delivery  of  pharmaceutical  drugs  to  specific  sites  in  the  intestine,  using  a  pH  sensitive
controlled  release  platform  technology.  Under  the  agreement,  Therabiome  granted  to  Assembly  the  exclusive  worldwide  license,  with  rights  to
sublicense, to develop the intellectual property for commercialization (a) in the use of bacteria, viruses, proteins and small molecules by oral delivery in
(i) gastro- intestinal dysbiosis, including but not limited to C. difficile, irritable bowel syndrome-constipation and inflammatory bowel disease, (ii) auto-
immune  disorders  and  autism,  including  but  not  limited  to  as  controlled  by  bacteria  or  virus,  and  (iii)  orally  delivered  vaccines,  including  viral  and
bacterial, and (b) any oral delivery of small molecules using the licensed intellectual property. Assembly will be solely responsible for all research and
development activities with respect to any product it develops under the license. 

For the license, Assembly paid Therabiome an upfront non-refundable license fee of $300,000. Assembly must pay Therabiome clinical and regulatory
milestones for each product or therapy advanced from the platform for U.S. regulatory milestones. Assembly also must pay Therabiome lesser amounts
for foreign regulatory milestones, which vary by country and region. Assembly also must pay Therabiome royalties on annual net sales of a product in
the low to mid-single digit percentages plus, once annual net sales exceed two certain thresholds, a one-time cash payment upon reaching each threshold.

Therabiome must pay Assembly royalties on annual net sales of any product it develops, using the intellectual property, in the low double to mid-double 
digit percentages, depending on the level of development or involvement Assembly had in the product. 

F-20  
  
  
  
  
  
  
  
  
  
  
  
 
 
 
 
 
Diltiazem (VEN 307) and Phenylepherine (VEN 308) 

ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

The Company had an exclusive royalty-bearing license agreement with S.L.A. Pharma, AG (“S.L.A. Pharma”) to sell, make and use diltiazem (VEN
307) for treatment, through topical administration, of anal fissures and phenylepherine (VEN 308) for treatment, through topical administration, of fecal
incontinence  (referred  to  collectively  as  the  “Compound  Technologies”)  in  the  United  States,  Canada  and  Mexico.  In  the  event  that  the  Compound
Technologies were commercialized, Assembly was obligated to pay to S.L.A. Pharma annual royalties, based upon net sales of the products. In addition,
Assembly  was  required  to  make  payments  to  S.L.A.  Pharma  up  to  an  aggregate  amount  of  $20  million  upon  the  achievement  of  various  milestones
related to regulatory events. 

On  July  24,  2014,  the  Company  notified  S.L.A.  Pharma  that  it  was  terminating  the license  agreement.  The  termination  was  effective  on  October 22,
2014. There were no early termination penalties as a result of the termination and the Company has no continuing obligation to make payment to S.L.A.
Pharma under the agreement. The Company terminated the agreement to focus on the development of its potentially curative programs for HBV, which
program  was  acquired  on  July  11,  2014  in  the  merger  with  Assembly  Pharmaceuticals,  Inc.,  and  CDI,  which  was  licensed  in  November  2013  from
Therabiome, LLC. 

Note 11 - Commitments and Contingencies 

Real Property Leases 

The Company leases office space for corporate and administrative functions in New York, NY under an  agreement with a monthly lease payment of
$10,130 that expires in August 2016. The Company also leases office space in Carmel, IN under a lease agreement that expires in June 2021. The leased
locations in New York, NY and Carmel, IN. are for corporate and administrative functions supporting both the HBV and Microbiome Programs and are
adequate for the Company’s current needs. 

The Company leases office and laboratory space in San Francisco, California under a sublease that expires in December 2017. The Company believes
these leased facilities are adequate for our current needs and that additional space will be available in the future on commercially reasonable terms as
needed. Research activities for the HBV program are also being conducted at laboratory space leased from Indiana University at Bloomington, IN. The
Company believes these leased facilities are adequate for the Company’s current needs. 

The total leasing expenses for the years ended December 31, 2015 and 2014 were approximately $0.7 and $0.2 million, respectively. 

Future minimum rental payments required as of December 31, 2015 are as follows: 

Year Ended December 31, 2016
Year Ended December 31, 2017
Year Ended December 31, 2018
Year Ended December 31, 2019
Year Ended December 31, 2020
Thereafter

  $

  $

1,086,783
976,547
104,093
106,360
108,627
55,353 
2,437,763 

Equipment Lease 

Pursuant to a Master Lease agreement dated November 25, 2014, the Company is leasing certain laboratory equipment. The equipment lease expense for
the year ended December 31, 2015 and 2014 amounted to $107,000 and $18,928, respectively. 

Employment Agreements 

On  January  15,  2014,  the  Company  entered  into  an  employment  agreement  with  its  Chief  Financial  Officer,  with  an  effective  date  of  December  22,
2013. The agreement has a term of two years and will be automatically extended for additional one-year periods unless the Company notifies the officer 
at least 180 days prior to the then current expiration date that it intends to not extend the employment agreement. The employment agreement initially
provided for a base salary of $300,000 for the Chief Financial Officer, and an annual discretionary bonus of up to 50% of the officer’s base salary based 
on  financial,  clinical  development  and  business  milestones  established  by  the  Board  of  Directors.  In  December  2014,  the  compensation  committee
approved a change of base salary to $350,000 per year for the Chief Financial Officer. On February 10, 2015, the Company named Mr. Barrett its Chief
Operating Officer, in addition to Chief Financial Officer. Mr. Barrett received a 3% salary increase, bringing his base salary to $360,500, in connection
with this additional responsibility. 

F-21  
  
  
  
  
  
  
  
  
  
  
  
  
  
   
   
   
   
   
 
ASSEMBLY BIOSCIENCES, INC. AND SUBSIDIARY 
Notes to Consolidated Financial Statements 

In  connection  with  the  Merger,  effective  July  11,  2014,  the  Company  entered  into  employment  agreements  with  its  President  and  Chief  Operating
Officer, its Chief Medical Officer, and its Chief Scientific Officer. In February 2015, Mr. Small was appointed as our President and Chief Executive
Officer and relinquished his title of Chief Operating Officer, and in January 2016, Dr. Arnold was appointed as our Chief Discovery Officer and Vice
President, Research and Development. The President’s employment agreement has a term of two years and will be automatically extended for additional
one-year periods unless the Company notifies the President at least 180 days prior to the then current expiration date that it intends to not extend the
employment agreement. The other two employment agreements provide for at-will employment, subject to payment of severance benefits depending on
the circumstances of termination. The employment agreements initially provided for a base salary of $350,000 per year for the President (increased to
base salary of $420,000 in February 2015), $290,000 per year for the Chief Medical Officer (increased to base salary of $319,000 in 2015) and $315,000
per year for the Chief Scientific Officer. Each employee is also eligible for an annual discretionary bonus based on achievement of financial, clinical
development and business milestones established by the Board of Directors, with the President eligible for a bonus of up to 50% of his base salary, and
the Chief Medical Officer and the Chief Scientific Officer eligible for a bonus of up to 30% of their respective base salaries. In 2014, the President and
the  Chief  Medical  Officer  also  received  a  retention  bonus  payable  after  three  months  of  employment  in  the  amount  of  $150,000  and  $100,000,
respectively. 

The Company has employment agreements with its Chief Executive Officer and Chief Financial Officer/Chief Operating Officer which provide for an
aggregate annual base salary of approximately $780,500 in 2015. 

Litigation 

The Company is not a party to any material legal proceedings and is not aware of any claims or actions pending or threatened against it. From time to 
time, the Company may be subject to various legal proceedings and claims that arise in the ordinary course of its business activities. 

Note 12 - Subsequent Event 

Effective January 5, 2016, the Company entered into an employment agreement with its new Chief Scientific Officer. The agreement has a term of five
years  and  will  be  automatically  extended  for  additional  one-year  periods  unless  the  Company  notifies  the  officer  at  least  180  days  prior  to  the  then
current  expiration  date  that  it  intends  to  not  extend  the  employment  agreement.  The  employment  agreement  initially  provides  for  a  base  salary  of
$380,000  per  year.  The  Chief  Scientific  Officer  is  also  eligible  for  an  annual  discretionary  bonus  based  on  achievement  of  financial,  clinical
development and business milestones established by the Board of Directors, with the Chief Scientific Officer eligible for a bonus of up to 35% of his
base salary. The Chief Scientific Officer also received a signing bonus payable after 30 days of employment in the amount of $75,000, which signing
bonus  is  subject  to  repayment  in  the  event  his  employment  terminates  for  certain  reasons  prior  to  January  5,  2017.  The  employment  agreement  also
provides for severance in connection with the termination of employment. 

On  January  9,  2016,  the  Company  entered  into  an  at-will  employment  letter  agreement  with  its  new  Chief  Development  Officer  and  Head  of
Microbiome  Program.  The  employment  agreement  provides  for  at-will  employment,  subject  to  payment  of  severance  benefits  depending  on  the
circumstances of termination. The employment agreements initially provided for a base salary of $360,000 per year and the employee is also eligible for
an annual discretionary bonus based on achievement of financial, clinical development and business milestones established by the Board of Directors of
up to 35% of his base salary. 

F-22  
  
  
  
  
  
  
CORPORATE INFORMATION 

Directors  

Officers 

Anthony E. Altig 
Chief Financial Officer, Biotix Holdings, Inc. 

Derek A. Small 
President and Chief Executive Officer 

Mark Auerbach 
Director, RCS Capital Corporation 

Richard DiMarchi, PhD 
Cox Distinguished Professor of Biochemistry 
and Gill Chair in Biomolecular Sciences  

Myron Z. Holubiak 
President, Leonard +Meron Biosciences, Inc. 

Alan J. Lewis, PhD 
Chief Executive Officer, DiaVacs, Inc. 

William Ringo 
Director Sangamo BioSciences, Inc.                     
Mirati Therapeutics, Immune Design Corp.,                     
Dermira and Five Prime Therapeutics 

David J. Barrett, C.P.A. 
Chief Financial Officer and Chief Operating 
Officer 

Uri Lopatin, M.D. 
Chief Medical Officer and Vice President of 
Research and Development 

Lee D. Arnold, M.D. 
Chief Discovery Officer 

Richard Colonno, PhD 
Chief Scientific Officer 

Thomas E. Rollins 
Chief Development Officer and Head of 
Microbiome Program 

Derek A. Small 
President and Chief Executive Officer, 
Assembly Biosciences, Inc. 

Aim 
Aim 

Headquarters 
101 Sixth Avenue, Ninth Floor 
New York, New York 10013 
855.971.4467 

Website 
www.assemblybio.com  

Stock Listing 
Assembly Biosciences, Inc. common stock is 
listed on the Nasdaq Capital Market and quoted 
under the symbol “ASMB” 

Transfer Agent 
VStock Transfer, LLC  
18 Lafayette Place 
Woodmere, New York 11598  
212.828.8436 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
www.assemblybio.com